Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc
|
|
- Clifton Evans
- 5 years ago
- Views:
Transcription
1 Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc Supplemental Figure S1. Phylogenetic tree of MYC2-related proteins from Arabidopsis and other plants. Phenogram representation of the Maxima Parsimonia method for 17 Arabidopsis, 1 Populus, 1 Rice and 1 Physcomitrella MYC2 bhlh-related proteins. Sequenced alignment was generated using multiple alignment ClustalW algorithm (T-Coffee web server, ( and the tree was created by MEGA4 program. Bootstrap values based on 100 replicates (from trials) are shown. The alignment used to generate this tree is available as Supplemental Data set 1 online. 1
2 Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc pdest32 JAZ12 JAZ11 JAZ10 JAZ9 JAZ8 JAZ7 JAZ6 JAZ5 JAZ4 JAZ3 JAZ2 prey JAZ1 bait MYC2 MYC3 1 MYC4 bhlh028 MYC2 1/10 MYC3 MYC4 bhlh028 Supplemental Figure 2. Control growth of yeast cells transformed with MYC3 or MYC4 and with JAZ repressors in yeast two-hybrid assays. Yeast cells co-transformed with pdest22-myc2, pdest22-myc3, pdest22-myc4 or pdest22-bhlh028 (preys) and pdest32-jaz1-12 (baits) were selected and subsequently grown on yeast synthetic dropout lacking Leu and Trp (-2), as a transformation control. 2
3 Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc JID bhlh A * MYC2 MYC3 MYC4 bhlh028 Os10g Populus P.patens Consensus JID (JAZ interaction domain) Activation Domain 188 B At1g32640/MYC2 At5g46760/MYC3 At4g17880/MYC4 At5g46830/BHLH028 AT5G41315/GL3 At1g63650/EGL3 AT4G09820/TT8 At1g01260/BHLH013 At2g46510/ATAIB At4g16430/BHLH003 consensus 93 JID 188 Figure S3. Alignment of the JAZ-interaction domain of MYC2-related proteins. (A) Aligned sequences of JAZ interacting domain of MYC2, MYC3, MYC4 and At5g46830 from Arabidopsis and MYC2-closest relatives from Rice (NM_ ), Populus (XM_ ) and Physcomitrella patens (XM_ ). The alignment was created with T-Coffee web server ( using the multiple aligment ClustalW algorithm. Residues conserved in all three proteins are labelled by asterisks, whereas dots indicate residues belonging to the same functional group. The non-conservative change (G-K) in At5g46830 is highlighted with a red square and a red asterisk. 3 (B) Sequences of the JAZ-interaction domain (JID) of several bhlh proteins of A. thaliana: MYC2, MYC3, MYC4,At5g46830 (BHLH028), At5g41315 (GL3), At1g63650 (EGL3), At4g09820 (TT8), At1g01260 (BHLH013), At2g46510 (AtAIB) and At4g16430 (BHLH003). Sequence alignment was generated as in (A).
4 Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc S:MYC3-GFP 35S:MYC4-GFP Supplemental Figure 4. MYC3 and MYC4 are nuclear-localized bhlh. Confocal microscope visualization of nuclear-localized MYC4-GFP and MYC3- GFP fusion proteins in the roots of 35S:MYC4-GFP (upper panels) and 35S:MYC3-GFP (lower panels) transgenic Arabidopsis seedlings. Superimposed images from UV (left panels) and visible lights allow the co-localization of GFP with nuclei of Arabidopsis root cells (right panels). 4
5 Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc MYC Relative Expression MYC2 MYC3 MYC4 WT 1h WT 6h WT 24h Supplemental Figure 5. JA dependent induction of MYC3 and MYC4 gene expression. Quantitative RT-PCR analysis of MYC2, MYC3 and MYC4 JA-dependent induction. 8- day-old WT (Col0) seedlings were assayed for the expression of these three genes in a timecourse experiment (1, 6 and 24 hours) in response to 50 µm JA. The measurements (three technical replicates) represent the ratio between mock and treated (50 µm JA) plants and the level of each gene is relative to ACTIN8. Error bars represent standard deviation. 5
6 Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc MYC2 MYC3 MYC4 Supplemental Figure 6. MYC2, MYC3 and MYC4 tissue expression patterns. Genevestigator ( com) array data of MYC2, MYC3 and MYC4 expression levels in different Arabidopsis thaliana tissues and developmental stages. The tissue specific expression of MYC3 and MYC4 is in agreement with our experimental data (described in figure 6 and highlighted here in black boxes). 6
7 Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc A 1,2 MYC2 Relative Expression 1,0 0,8 0,6 0,4 0,2 * B MYC3 Relative Expression 0,0 1,2 1,0 0,8 0,6 0,4 0,2 WT myc3 myc4 * * * * 0,0 WT myc2 myc4 C MYC4 Relative Expression 1,4 1,2 1,0 0,8 0,6 0,4 0,2 0,0 * * *** *** WT myc2 myc3 Supplemental Figure 7. Expression of MYC genes in myc mutant backgrounds. MYC2, MYC3 and MYC4 expression was analyzed in WT (Col-0) and mutant backgrounds myc2, myc3 and myc4. Quantitative RT-PCR measurements were carried out in 8-days-old seedlings growing in control media. Measurements (three technical replicates) represent the level of each gene relative to ACTIN8 normalized to WT (Col-0) values. Error bar represent standard deviation and asterisks indicate statistically significant differences compared to Col-0 (Student's t test, * P< 0.05, ** P< 0.01, *** P< 0.001).. 7
8 Supplemental Data. Fernández-Calvo et al. Plant Cell. ( /tcp Supplemental Table 1. MALDI-TOF/TOF-MS identification of MYC2, MYC3, MYC4, JAZ3 and JAZ5 interactors. Bait Prey PMF data MSMS data # Found/ Database Protein RMS error Sequence Unique Total Peptide Variable Bait Prey Locus Prey Name # exp version Score Expect (ppm) coverage % Peptides Ion Score Number Start End Observed Mr(Exp) Mr(Calc) Delta Miss Ions Score Expect Peptide Modification MYC2 AT1G32640 MYC2 (BAIT) 3/3 TAIR ,00E ,42 884,41 884,41 0, ,70E-04 K.FNNTFSR.E , , ,55 0, ,00E-04 R.IYTQEQLR.A , , ,56 0, ,10E-01 K.IIGWDAMIR.V Oxidation (M) , , ,69 0, ,60E-07 R.SGEILNFGDEGKR.S , , ,82 0, ,40E-06 K.NQLEEVKLELAGR.K , , ,87-0, ,40E-10 K.ASLLGDAIAYINELK.S , , ,97 0, ,30E-10 R.SSGNPDPSSYSGQTQFENKR.K , , ,05 0, ,70E-06 K.AFATGNAVWVSGSDQLSGSGCER.A , , ,47 0, ,70E-08 K.QGGVFGMHTIACIPSANGVVEVGSTEPIR.Q MYC2 AT5G46760 MYC3 3/3 TAIR ,30E , , ,56 0, ,21E-05 R.AGQGQIYGLK.T , , ,60-0, ,10E-06 K.DLTFQGGLLK.S , , ,60 0, ,50E-02 K.IIGWDVMIR.V , , ,60 0, ,09E-01 K.IIGWDVMIR.V Oxidation (M) , , ,67-0, ,40E-03 K.MGSQFFNHDQLK.V , , ,66-0, ,51E-03 K.MGSQFFNHDQLK.V Oxidation (M) , , ,86 0, ,94E-06 K.ASLLGDAISYINELK.S , , ,78 0, ,08E-02 K.GSNNDEGMLSFSTVVR.S , , ,85 0, ,08E-05 R.VIWLSGSGALTGSGCER.A MYC2 AT4G28910 NINJA 3/3 TAIR ,60E ,48 895,48 895,48 0, ,43E-03 R.TISGVTYR.Y , , ,81 0, ,61E-08 K.DGSGGIVALSQSPFAGR.V , , ,86 0, ,97E-02 R.SDSGQQPPQNFFNDLSK.A , , ,10 0, ,74E-01 K.EVVRPPTDTNIVDNLTGQR.R MYC2 AT5G20900 JAZ12 3/3 TAIR ,30E , , ,49 0, ,62E-03 K.NPYPTSDFK.K , , ,59 0, ,91E-04 K.NPYPTSDFKK.T , , ,78 0, ,11E-05 K.NSTSISPVSSPALNR.A , , ,89 0, ,52E-02 K.TDVPTGNVSIKEEFPTA , , ,40 0, ,17E+00 R.APSFSSTSNVASPAAQPFPIQPISFCR.S MYC2 AT1G74950 JAZ2 2/3 TAIR ,30E , , ,58 0, ,83E-02 R.KPSFSQTCTR.L , , ,01 0, ,08E-04 K.SVKPESQSAPLTIFYGGR.V MYC2 AT3G43440 JAZ11 1/3 TAIR8 60 3,40E , , ,64 0, ,83E-03 K.NVTGINPALNR.A , , ,96 0, ,65E-01 R.STEPDASTQLTIIFGGSCR.V MYC3 AT5G46760 MYC3 (BAIT) 4/4 TAIR ,10E , , ,56 0, ,10E-06 R.AGQGQIYGLK.T , , ,60 0, ,60E-07 K.DLTFQGGLLK.S , , ,60-0, ,20E-04 K.IIGWDVMIR.V , , ,60 0, ,50E-01 K.IIGWDVMIR.V Oxidation (M) , , ,67 0, ,10E-06 K.MGSQFFNHDQLK.V , , ,66 0, ,20E-04 K.MGSQFFNHDQLK.V Oxidation (M) , , ,86 0, ,00E-09 K.ASLLGDAISYINELK.S , , ,78 0, ,90E-11 K.GSNNDEGMLSFSTVVR.S , , ,78 0, ,50E-08 K.GSNNDEGMLSFSTVVR.S Oxidation (M) , , ,85 0, ,40E-13 R.VIWLSGSGALTGSGCER.A , , ,98 0, ,70E-07 R.KPANGREEPLNHVEAER.Q MYC3 AT5G20900 JAZ12 4/4 TAIR ,30E , , ,49 0, ,50E-04 K.NPYPTSDFK.K , , ,59 0, ,20E-05 K.NPYPTSDFKK.T , , ,78 0, ,40E-07 K.NSTSISPVSSPALNR.A , , ,89 0, ,00E-06 K.TDVPTGNVSIKEEFPTA , , ,06 0, ,40E-14 R.ASVEGGCGVADGDGGAAEIGGTGSVEK.S , , ,40 0, ,60E-06 R.APSFSSTSNVASPAAQPFPIQPISFCR.S MYC3 AT4G28910 NINJA 4/4 TAIR ,20E , , ,81 0, ,80E-12 K.DGSGGIVALSQSPFAGR.V , , ,87-5, ,40E-06 R.SDSGQQPPQNFFNDLSK.A , , ,88 1, ,40E-01 K.IVCACHGSHMSPEEFVR.H Oxidation (M) , , ,10-3, ,20E-06 K.EVVRPPTDTNIVDNLTGQR.R , , ,13-2, ,50E-01 K.FGGSGARPNLPWVSTTGSGPHGR.T MYC3 AT1G74950 JAZ2 4/4 TAIR ,20E , , ,58 0, ,50E-01 R.KPSFSQTCTR.L , , ,58 0, ,60E-05 R.VMVFDDFSAEK.A Oxidation (M) , , ,01 0, ,30E-10 K.SVKPESQSAPLTIFYGGR.V , , ,98 0, ,60E-01 K.GSFGDLSLGMTCKPDVNGGSR.Q Oxidation (M) , , ,96 0, ,10E+00 K.SFTCFTAEVNNNHSAYSQK.E , , ,20 0, ,50E-05 K.TAAQEPIQPNPASLACELPIAR.R , , ,22 0, ,40E-05 K.APYQIDGSAEASSKPTNPAWLSSR.- MYC3 AT4G17880 MYC4 2/4 TAIR ,60E , , ,57 0, ,80E-04 K.IIGWDAMIR.I , , ,56 0, ,20E+00 K.IIGWDAMIR.I Oxidation (M) , , ,98 0, ,40E-03 R.KPANGREEPLNHVEAER.Q , , ,40 0, ,10E-04 K.GTGLPGQAFSNSDTIWLSGSNALAGSSCER.A MYC4 AT4G17880 MYC4 (BAIT) 4/4 TAIR ,70E , , ,57-0, ,20E-05 K.IIGWDAMIR.I , , ,56-0, ,10E-02 K.IIGWDAMIR.I Oxidation (M) , , ,68-3, ,10E-06 K.MGNQFFTQDQLK.V Oxidation (M) , , ,85-3, ,30E-11 K.ASLLGDAISYISELK.S , , ,89-2, ,50E-08 M.SPTNVQVTDYHLNQSK.T , , ,40 3, ,60E-10 K.GTGLPGQAFSNSDTIWLSGSNALAGSSCER.A MYC4 AT5G20900 JAZ12 4/4 TAIR ,30E , , ,49-2, ,10E-07 K.NPYPTSDFK.K , , ,60-1, ,00E-05 K.TDVPTGNVSIK.E , , ,59-1, ,30E-06 K.NPYPTSDFKK.T , , ,78-0, ,00E-10 K.NSTSISPVSSPALNR.A , , ,89-0, ,70E-09 K.TDVPTGNVSIKEEFPTA , , ,06 4, ,20E-10 R.ASVEGGCGVADGDGGAAEIGGTGSVEK.S , , ,40 3, ,60E-03 R.APSFSSTSNVASPAAQPFPIQPISFCR.S 8
9 MYC4 AT5G46760 MYC3 4/4 TAIR ,50E , , ,56 0, ,90E-05 R.AGQGQIYGLK.T , , ,60 0, ,00E-01 K.IIGWDVMIR.V , , ,60 0, ,20E-02 K.IIGWDVMIR.V Oxidation (M) , , ,66 0, ,00E-02 K.MGSQFFNHDQLK.V Oxidation (M) , , ,86 0, ,00E-08 K.ASLLGDAISYINELK.S , , ,78 0, ,30E-01 K.GSNNDEGMLSFSTVVR.S , , ,78 0, ,20E-04 K.GSNNDEGMLSFSTVVR.S Oxidation (M) , , ,85 0, ,00E-10 R.VIWLSGSGALTGSGCER.A , , ,05-0, ,40E-05 R.QSSCLVEKDLTFQGGLLK.S , , ,42 0, ,90E+00 K.ELDLEVNHASLSVVNDLMIQQATVK.M Oxidation (M) MYC4 AT4G28910 NINJA 4/4 TAIR ,10E , , ,81 0, ,60E-12 K.DGSGGIVALSQSPFAGR.V , , ,86-0, ,30E-05 R.SDSGQQPPQNFFNDLSK.A , , ,10 0, ,70E-04 K.EVVRPPTDTNIVDNLTGQR.R , , ,13-0, ,60E+00 K.FGGSGARPNLPWVSTTGSGPHGR.T , , ,15 0, ,20E-09 K.APTTEAEASTKPLWVEDESRK.E MYC4 AT1G74950 JAZ2 3/4 TAIR ,60E , , ,58 0, ,20E-04 R.KPSFSQTCTR.L , , ,58 0, ,70E-05 R.VMVFDDFSAEK.A Oxidation (M) , , ,01 0, ,70E-08 K.SVKPESQSAPLTIFYGGR.V , , ,22 0, ,20E-03 K.APYQIDGSAEASSKPTNPAWLSSR.- MYC4 AT3G43440 JAZ11 4/4 TAIR ,30E , , ,64 0, ,40E-04 K.NVTGINPALNR.A , , ,96 0, ,90E-11 R.STEPDASTQLTIIFGGSCR.V JAZ3 AT3G17860 JAZ3 (BAIT) 2/2 TAIR ,70E , , ,55 0, ,10E-08 K.APYSSVQGVR.M , , ,64 1, ,10E-08 R.KAPYSSVQGVR.M , , ,99-2, ,10E-03 K.VSASSSQFLSFRPTQEDR.H , , ,20-1, ,30E-04 K.SGNYHLPHSGSFMPSSVADVYDSTR.K Oxidation (M) , , ,57 7, ,90E-02 R.SSSKPIGSPAQLTIFYAGSVCVYDDISPEK.A JAZ3 AT4G28910 NINJA 2/2 TAIR ,30E , , ,63-1, ,20E-09 R.SNHGGSGTEEFTMR.N , , ,63-0, ,40E-02 R.SNHGGSGTEEFTMR.N Oxidation (M) , , ,81 0, ,80E-13 K.DGSGGIVALSQSPFAGR.V , , ,87-7, ,90E-06 R.SDSGQQPPQNFFNDLSK.A , , ,88-0, ,10E-03 K.IVCACHGSHMSPEEFVR.H Oxidation (M) , , ,10-2, ,90E-06 K.EVVRPPTDTNIVDNLTGQR.R , , ,13-1, ,10E+00 K.FGGSGARPNLPWVSTTGSGPHGR.T JAZ3 AT5G46760 MYC3 1/2 TAIR ,70E , , ,60-1, ,10E-02 K.IIGWDVMIR.V , , ,60-2, ,10E-01 K.IIGWDVMIR.V Oxidation (M) , , ,86-9, ,60E-03 K.ASLLGDAISYINELK.S , , ,78-3, ,30E-01 K.GSNNDEGMLSFSTVVR.S Oxidation (M) , , ,85 3, ,00E-02 R.VIWLSGSGALTGSGCER.A JAZ3 AT1G80490/AT1G15750 TPR1/TOPLESS 1/2 TAIR9 / / / / / , , ,80-10, ,20E-03 K.EITQLLTLENFR.E JAZ5 AT1G17380 JAZ5 (BAIT) 4/4 TAIR ,60E , , ,66 0, ,70E-08 K.GSFGNIDLGLYR.K , , ,69 0, ,00E-04 K.VLVYNEFPVDK.A , , ,71-0, ,70E-08 R.APYQVNQNAGHHR.Y , , ,80-0, ,40E-03 K.EKGSFGNIDLGLYR.K , , ,84-0, ,80E-08 K.EQQQQQEQNQIVER.I , , ,02-0, ,50E-07 K.SSMVLPDLNEPTDNNHLTK.E , , ,01-0, ,30E-07 K.SSMVLPDLNEPTDNNHLTK.E Oxidation (M) , , ,25 0, ,60E-07 K.QAKPVTEINIQTPINDENNNNK.S JAZ5 AT4G28910 NINJA 4/4 TAIR ,30E , , ,63 0, ,50E-08 R.SNHGGSGTEEFTMR.N , , ,81 0, ,60E-12 K.DGSGGIVALSQSPFAGR.V , , ,86 0, ,80E-03 R.SDSGQQPPQNFFNDLSK.A , , ,10 0, ,10E-03 K.EVVRPPTDTNIVDNLTGQR.R , , ,13 0, ,90E-04 K.FGGSGARPNLPWVSTTGSGPHGR.T , , ,15-0, ,80E-10 K.APTTEAEASTKPLWVEDESRK.E , , ,39 0, ,80E-01 K.VIDDFKNFLHPTSQRPAEPSSGSQR.S JAZ5 AT1G15750 TOPLESS 2/4 TAIR ,30E , , ,61 0, ,80E-04 K.LIEANPLFR.D , , ,63 0, ,10E-05 R.DKLQFPTLR.N , , ,54 0, ,20E-03 K.AWLYDNMGSR.V , , ,67-0, ,90E-04 K.VFSTFNEELFK.E , , ,80 0, ,00E-07 K.EITQLLTLENFR.E , , ,80 0, ,90E-06 K.ENIQFIFSTALDGK.I , , ,20-0, ,10E-04 K.QTLLLVGTNVGDIGLWEVGSR.E JAZ5 AT5G20900 JAZ12 4/4 TAIR ,00E , , ,59 0, ,70E-05 K.NPYPTSDFKK.T , , ,78-0, ,10E-06 K.NSTSISPVSSPALNR.A , , ,89 0, ,00E-06 K.TDVPTGNVSIKEEFPTA , , ,06 0, ,20E-12 R.ASVEGGCGVADGDGGAAEIGGTGSVEK.S , , ,40 0, ,30E-06 R.APSFSSTSNVASPAAQPFPIQPISFCR.S JAZ5 AT5G46760 MYC3 2/4 TAIR ,20E , , ,56 0, ,00E-05 R.AGQGQIYGLK.T , , ,60-0, ,60E-05 K.DLTFQGGLLK.S , , ,60 0, ,20E-02 K.IIGWDVMIR.V , , ,60 0, ,50E-02 K.IIGWDVMIR.V Oxidation (M) , , ,67 0, ,00E-03 K.MGSQFFNHDQLK.V , , ,66 0, ,90E-01 K.MGSQFFNHDQLK.V Oxidation (M) , , ,78 0, ,40E-04 K.GSNNDEGMLSFSTVVR.S , , ,78 0, ,20E-01 K.GSNNDEGMLSFSTVVR.S Oxidation (M) , , ,85 0, ,80E-03 R.VIWLSGSGALTGSGCER.A JAZ5 AT3G16830 TPR2 1/4 TAIR ,10E , , ,61 0, ,70E-05 K.LIEANPLFR.E , , ,80-0, ,20E-03 K.EITQLLTLENFR.E , , ,80 0, ,30E-05 K.ENIQFIFSTALDGK.I , , ,10 0, ,80E-03 R.LLYTNSGVGVLALGSNGVQR.L JAZ5 AT5G27030 TPR3 1/4 TAIR ,30E , , ,61 0, ,70E-05 K.LIEANPLFR.D , , ,80 0, ,30E-05 K.ENIQFIFSTAIDGK.I , , ,91 0, ,00E-03 K.HLIQLYAFSGPNDLR.Q JAZ5 AT1G74950 JAZ2 2/4 TAIR8 98 5,30E , , ,01 0, ,20E-04 K.SVKPESQSAPLTIFYGGR.V 9
10 Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc Supplemental Table2. List of primers used. Oligonucleotide sequences used within this work to amplify and genotype indicated genes. Primer Name Sequence 5 to 3 Used to LBGabiKat8409 ATATTGACCATCATACTCATTGC Genotyping LBGabiKat8760 GGGCTACACTGAATTGGTAGCTC Genotyping MYC3FwGKgenespecific AAGGTGGGTTGTTGAAATCTAATG Genotyping MYC3RvCDS TCAATAGTTTTCTCCGACTTTC Genotyping MYC4FwCDS ATGTCTCCGACGAATGTTCA Genotyping MYC4RvGKgeneespecific AACCAAATTCACCACCACCAT Genotyping MYC3Fwgateway ggggacaagtttgtacaaaaaagcaggcttcatgaacggcacaacatcatca Full lenght MYC3 MYC3Rvstopgateway ggggaccactttgtacaagaaagctgggttcaatagttttctccgacttt Full lenght MYC3 MYC3Rvnonstopgateway ggggaccactttgtacaagaaagctgggtcatagttttctccgactttt Full lenght MYC3 (no stop) MYC4Fwgateway ggggacaagtttgtacaaaaaagcaggcttcatgtctccgacgaatgttca Full lenght MYC4 MYC4Rvstopgateway ggggaccactttgtacaagaaagctgggttcatggacattctccaactttc Full lenght MYC4 MYC4Rvnonstopgateway ggggaccactttgtacaagaaagctgggtctggacattctccaactttc Full lenght MYC4 (no stop) At5g46830Fwgateway ggggacaagtttgtacaaaaaagcaggcttcatgattaataccgacgataac Full lenght At5g46830 At5g46830Rvstopgateway ggggaccactttgtacaagaaagctgggttcagctaattttcgacatcaa Full lenght At5g46830 At5g46830Rvnonstopgateway ggggaccactttgtacaagaaagctgggtcgctaattttcgacatcaa Full lenght At5g46830 (no stop) AtAIBFwgateway ggggacaagtttgtacaaaaaagcaggcttcatgaatatgagtgatttaggt Full lenght AtAIB AtAIBRvstopgateway ggggaccactttgtacaagaaagctgggtttatatatcaccagagacctgt Full lenght AtAIB AtAIBRvnonstopgateway ggggaccactttgtacaagaaagctgggtctatatcaccagagacctgt Full lenght AtAIB (no stop) MYC3TAPFw ggggacaagtttgtacaaaaaagcaggctccaccatgaacggcacaacatcatcaatc MYC3TAP clone MYC4TAPFw ggggacaagtttgtacaaaaaagcaggctccaccatgtctccgacgaatgttcaag MYC4TAP clone JAZ3TAPFw ggggacaagtttgtacaaaaaagcaggctccaccatggagagagattttctcgggttgg JAZ3TAP clone JAZ5TAPRv ggggacaagtttgtacaaaaaagcaggctccaccatgtcgtcgagcaatgaaaatgc JAZ5TAP clone MYC3promFw cacctgttattagcgcaaagaggatcg pmyc3:gus MYC3promRv GAAGTTGATTGATGATGTTGTGCCGTTCAT pmyc3:gus MYC4promFw caccacagtactaacgtttgatggaaacat pmyc4:gus MYC4promRv ATCGGTTACTTGAACATTCGTCGGAGACAT pmyc4:gus PDF1.2qPCRFw AGTTGTGCGAGAAGCCAAGT PDF1.2 qpcr amplification PDF1.2qPCRRv GTTGCATGATCCATGTTTGG PDF1.2 qpcr amplification VSP2qPCRFw CGTCGATTCGAAAACCATCT VSP2 qpcr amplification VSP2qPCRRv GGCACCGTGTCGAAGTCTAT VSP2 qpcr amplification JAZ10qPCRFW GAGAAGCGCAAGGAGAGATTAG JAZ10 qpcr amplification JAZ10qPCRRv CTTAGTAGGTAACGTAATCTCC JAZ10 qpcr amplification MYC2qPCRFw GTGCGGGATTAGCTGGTAAA MYC2 qpcr amplification MYC2qPCRRv ATGCATCCCAAACACTCCTC MYC2 qpcr amplification MYC3qPCRFw TGTTGAAGCAGAGAGGCAGA MYC3 qpcr amplification MYC3qPCRRv CTCCGAGAAGCGAAGCTTTA MYC3 qpcr amplification MYC4qPCRFw AGGAGCAAACGAGAACTGGA MYC4 qpcr amplification MYC4qPCRRv CCATCTCCCCAACCTAACAA MYC4 qpcr amplification 10
11 ACTIN-8qPCRFw CCAGTGGTCGTACAACCGGTA ACTIN8 qpcr amplification ACTIN-8qPCRRv TAGTTCTTTTCGATGGAGGAGCTG ACTIN8 qpcr amplification MYC2N-term-attB1-F1old ggggacaagtttgtacaaaaaagcaggcttcatggagattccggcacaggcg MYC2N MYC2N-term-attB2-R1 ggggaccactttgtacaagaaagctgggtcggaggcgccggagaaatcata MYC2N MYC2midFw ggggacaagtttgtacaaaaaagcaggctcgggattagctggtaaagcg MYC2-mid MYC2midRv ggggaccactttgtacaagaaagctgggtcttaatgaaccggactcggagt MYC2-mid MYC2NTFw ggggacaagtttgtacaaaaaagcaggcttcatggagattccggcacaggcg MYC2-Nt MYC2NTRv ggggaccactttgtacaagaaagctgggtctcacgctttaccagctaatcccg MYC2-Nt MYC2Fw55 ggggacaagtttgtacaaaaaagcaggcttcatggagattccggcacaggcg MYC2-D55-99 MYC2Rv99 ggggaccactttgtacaagaaagctgggtcggaggcgccggagaaatcata MYC2-D55-99 MYC2Fw93 ggggacaagtttgtacaaaaaagcaggcttcatgtatgatttctccggcgcctcc MYC2-D MYC2Rv160 ggggaccactttgtacaagaaagctgggtcctcctcatcaacagcgtcatc MYC2-D MYC3dom44Fw ggggacaagtttgtacaaaaaagcaggcttccagcctcagttcaacgaagat MYC3-D44-87 MYC3dom87Rv ggggaccactttgtacaagaaagctgggtcactgtgttatctccggtgga MYC3-D44-87 MYC3Fw82 ggggacaagtttgtacaaaaaagcaggcttcgacttcgattcatccaccgga MYC3-D MYC3Rv141 ggggaccactttgtacaagaaagctgggtcttcatcgtttgattcatcgga MYC3-D JAZ2FwEcoR1 GTCACGAATTCATGTCGAGTTTTTCTGCCG JAZ2pGBKT7 Y2H JAZ2RvPst-1 GACGTCTGCAGTTACCGTGAACTGAGCCAAG JAZ2pGBKT7 Y2H JAZ3FwEcoR1 CGGAATTCCGGTTCATGGAGAGAGAT JAZ3pGBKT7 Y2H JAZ3RvPst-1 CCAATGCATTGGTTCTGCAGTTTTAGGTTGC JAZ3pGBKT7 Y2H pdest32fw AACCGAAGTGCGCCAAGTGTCTG Check clones pdest32rv CAGAGGTTAGTTCCAACAGCCGA Check clones pdest22fw TATAACGCGTTTGGAATCACT Check clones pdest22rv AGCCGACAACCTTGATTGGAGAC Check clones T7 promoter (-20) TAATACGACTCACTATAGGG Check clones 11
Supplemental Data. Perea-Resa et al. Plant Cell. (2012) /tpc
Supplemental Data. Perea-Resa et al. Plant Cell. (22)..5/tpc.2.3697 Sm Sm2 Supplemental Figure. Sequence alignment of Arabidopsis LSM proteins. Alignment of the eleven Arabidopsis LSM proteins. Sm and
More informationSupplemental Data. Perrella et al. (2013). Plant Cell /tpc
Intensity Intensity Intensity Intensity Intensity Intensity 150 50 150 0 10 20 50 C 150 0 10 20 50 D 0 10 20 Distance (μm) 50 20 40 E 50 F 0 10 20 50 0 15 30 Distance (μm) Supplemental Figure 1: Co-localization
More information** * * * Col-0 cau1 CAU1. Actin2 CAS. Actin2. Supplemental Figure 1. CAU1 affects calcium accumulation.
Ca 2+ ug g -1 DW Ca 2+ ug g -1 DW Ca 2+ ug g -1 DW Supplemental Data. Fu et al. Plant Cell. (213). 1.115/tpc.113.113886 A 5 4 3 * Col- cau1 B 4 3 2 Col- cau1 ** * * ** C 2 1 25 2 15 1 5 Shoots Roots *
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:10.1038/nature10534 Supplementary Fig. 1. Diagrammatic representation of the N-end rule pathway of targeted proteolysis (after Graciet and Wellmer 2010 9 ). Tertiary, secondary
More informationSupplementary Figure S1. Amino acid alignment of selected monocot FT-like and TFL-like sequences. Sequences were aligned using ClustalW and analyzed
Supplementary Figure S1. Amino acid alignment of selected monocot FT-like and TFL-like sequences. Sequences were aligned using ClustalW and analyzed using the Geneious software. Accession numbers of the
More informationNature Genetics: doi: /ng Supplementary Figure 1. The phenotypes of PI , BR121, and Harosoy under short-day conditions.
Supplementary Figure 1 The phenotypes of PI 159925, BR121, and Harosoy under short-day conditions. (a) Plant height. (b) Number of branches. (c) Average internode length. (d) Number of nodes. (e) Pods
More informationSupplementary Figure 1
Supplementary Figure 1 Supplementary Figure 1. HSP21 expression in 35S:HSP21 and hsp21 knockdown plants. (a) Since no T- DNA insertion line for HSP21 is available in the publicly available T-DNA collections,
More informationSupplemental Figure 1. Phenotype of ProRGA:RGAd17 plants under long day
Supplemental Figure 1. Phenotype of ProRGA:RGAd17 plants under long day conditions. Photo was taken when the wild type plant started to bolt. Scale bar represents 1 cm. Supplemental Figure 2. Flowering
More informationSupplementary Figure 1. Phenotype of the HI strain.
Supplementary Figure 1. Phenotype of the HI strain. (A) Phenotype of the HI and wild type plant after flowering (~1month). Wild type plant is tall with well elongated inflorescence. All four HI plants
More informationSUPPLEMENTARY INFORMATION
reverse 3175 3175 F L C 318 318 3185 3185 319 319 3195 3195 315 8 1 315 3155 315 317 Supplementary Figure 3. Stability of expression of the GFP sensor constructs return to warm conditions. Semi-quantitative
More informationEthylene is critical to the maintenance of primary root growth and Fe. homeostasis under Fe stress in Arabidopsis
Ethylene is critical to the maintenance of primary root growth and Fe homeostasis under Fe stress in Arabidopsis Guangjie Li, Weifeng Xu, Herbert J. Kronzucker, Weiming Shi * Supplementary Data Supplementary
More informationSupplemental Table 1. Primers used for cloning and PCR amplification in this study
Supplemental Table 1. Primers used for cloning and PCR amplification in this study Target Gene Primer sequence NATA1 (At2g393) forward GGG GAC AAG TTT GTA CAA AAA AGC AGG CTT CAT GGC GCC TCC AAC CGC AGC
More informationSupplemental Data. Gao et al. (2012). Plant Cell /tpc
Supplemental Figure 1. Plant EMP Proteins. (A) The Accession numbers of the 12 EMP members from Arabidopsis. (B) Phylogenetic analysis of EMP proteins from Arabidopsis, human and yeast using the Mac Vector
More informationTable S1 List of primers used for genotyping and qrt-pcr.
Table S1 List of primers used for genotyping and qrt-pcr. genotyping! allele! ligomer*! 5'-sequence-3'! rice! d10-2! F! TTGGCTTTGCCTCGTTTC!!! R! AGCCTCCACTTGTACTGTG! Arabidopsis! max2-3, max2-4! F! ACTCTCTCCGACCTCCCTGACG!!!
More information** LCA LCN PCA
% of wild type value % of wild type value a 12 1 8 2 b 12 1 8 2 LCA LCN PCA Col- sod3-1 Supplementary Figure 1 sod3-1 influences cell proliferation. (a) Fifth leaf cell area (LCA) and leaf cell number
More informationGFP GAL bp 3964 bp
Supplemental Data. Møller et al. (2009) Shoot Na + exclusion and increased salinity tolerance engineered by cell type-specific alteration of Na + transport in Arabidopsis Supplemental Figure 1. Salt-sensitive
More informationSupplemental Data. Chen and Thelen (2010). Plant Cell /tpc
Supplemental Data. Chen and Thelen (2010). Plant Cell 10.1105/tpc.109.071837 1 C Total 5 kg 20 kg 100 kg Transmission Image 100 kg soluble pdtpi-gfp Plastid (PDH-alpha) Mito (PDH-alpha) GFP Image vector
More informationSupplemental Data. Wang et al. (2014). Plant Cell /tpc
Supplemental Figure1: Mock and NPA-treated tomato plants. (A) NPA treated tomato (cv. Moneymaker) developed a pin-like inflorescence (arrowhead). (B) Comparison of first and second leaves from mock and
More informationPenghui Li, Beibei Chen, Gaoyang Zhang, Longxiang Chen, Qiang Dong, Jiangqi Wen, Kirankumar S. Mysore and Jian Zhao
New Phytologist Supporting Information Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula bhlh transcription factor MtTT8 Penghui Li, Beibei Chen, Gaoyang Zhang, Longxiang
More informationFigure 1. Identification of UGT74E2 as an IBA glycosyltransferase. (A) Relative conversion rates of different plant hormones to their glucosylated
Figure 1. Identification of UGT74E2 as an IBA glycosyltransferase. (A) Relative conversion rates of different plant hormones to their glucosylated form by recombinant UGT74E2. The naturally occurring auxin
More informationSupporting Online Material for
www.sciencemag.org/cgi/content/full/1121356/dc1 Supporting Online Material for Polar PIN Localization Directs Auxin Flow in Plants Justyna Wiśniewska, Jian Xu, Daniela Seifertová, Philip B. Brewer, Kamil
More informationSupplementary Figure 1 Characterization of wild type (WT) and abci8 mutant in the paddy field.
Supplementary Figure 1 Characterization of wild type (WT) and abci8 mutant in the paddy field. A, Phenotypes of 30-day old wild-type (WT) and abci8 mutant plants grown in a paddy field under normal sunny
More informationSupplementary Materials for
www.sciencesignaling.org/cgi/content/full/9/452/ra106/dc1 Supplementary Materials for Stem-piped light activates phytochrome B to trigger light responses in Arabidopsis thaliana roots Hyo-Jun Lee, Jun-Ho
More informationNature Genetics: doi: /ng Supplementary Figure 1. ssp mutant phenotypes in a functional SP background.
Supplementary Figure 1 ssp mutant phenotypes in a functional SP background. (a,b) Statistical comparisons of primary and sympodial shoot flowering times as determined by mean values for leaf number on
More informationSupplemental Data. Hou et al. (2016). Plant Cell /tpc
Supplemental Data. Hou et al. (216). Plant Cell 1.115/tpc.16.295 A Distance to 1 st nt of start codon Distance to 1 st nt of stop codon B Normalized PARE abundance 8 14 nt 17 nt Frame1 Arabidopsis inflorescence
More informationSupplemental Data. Yang et al. (2012). Plant Cell /tpc
Supplemental Figure 1. Mature flowers of P. heterotricha. (A) An inflorescence of P. heterotricha showing the front view of a zygomorphic flower characterized by two small dorsal petals and only two fertile
More informationEST1 Homology Domain. 100 aa. hest1a / SMG6 PIN TPR TPR. Est1-like DBD? hest1b / SMG5. TPR-like TPR. a helical. hest1c / SMG7.
hest1a / SMG6 EST1 Homology Domain 100 aa 853 695 761 780 1206 hest1 / SMG5 -like? -like 109 145 214 237 497 165 239 1016 114 207 212 381 583 hest1c / SMG7 a helical 1091 Sc 57 185 267 284 699 Figure S1:
More informationRon et al SUPPLEMENTAL DATA
Ron et al SUPPLEMENTAL DATA Hairy root transformation using Agrobacterium rhizogenes as a tool for exploring cell type-specific gene expression and function using tomato as a model Mily Ron, Kaisa Kajala,
More information23-. Shoot and root development depend on ratio of IAA/CK
Balance of Hormones regulate growth and development Environmental factors regulate hormone levels light- e.g. phototropism gravity- e.g. gravitropism temperature Mode of action of each hormone 1. Signal
More informationSUPPLEMENTARY INFORMATION
PRC2 represses dedifferentiation of mature somatic cells in Arabidopsis Momoko Ikeuchi 1 *, Akira Iwase 1 *, Bart Rymen 1, Hirofumi Harashima 1, Michitaro Shibata 1, Mariko Ohnuma 1, Christian Breuer 1,
More informationSUPPLEMENTARY INFORMATION
doi:10.1038/nature09606 Chen Li-Qing et al. A novel class of sugar transporters. Supplementary Figure 1. Confocal imaging of FRET sensor localization in HEK293T cells. The subcellular localization of the
More informationDevelopment 143: doi: /dev : Supplementary information
Supplementary Materials and Methods Plant materials The mutants and transgenic plants used in the present study were as follows: E361 (from Alex Webb s laboratory); tmm-1, ptmm::tmm-gfp and flp-1 (from
More informationSupplemental Data. Yamamoto et al. (2016). Plant Cell /tpc WT + HCO 3. slac1-4/δnc #5 + HCO 3 WT + ABA. slac1-4/δnc #5 + ABA
I (pa) I (pa) A WT + HCO 3 B 2 V (mv) 15 1 5 5 2 slac14/δnc #5 + HCO 3 4 6 WT (n = 6) 8 WT + HCO 3 (n = 7) slac14/δnc #5 (n = 6) slac14/δnc #5 + HCO 3 (n = 1) C WT + ABA D 2 V (mv) 15 1 5 5 2 slac14/δnc
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:10.1038/nature12791 Supplementary Figure 1 (1/3) WWW.NATURE.COM/NATURE 1 RESEARCH SUPPLEMENTARY INFORMATION Supplementary Figure 1 (2/3) 2 WWW.NATURE.COM/NATURE SUPPLEMENTARY
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb3267 Supplementary Figure 1 A group of genes required for formation or orientation of annular F-actin bundles and aecm ridges: RNAi phenotypes and their validation by standard mutations.
More informationPYR1 PYL1 PYL2 PYL3 PYL4 PYL5 PYL6 PYL7 PYL8 PYL9 PYL10 PYL11/12
Supplemental Data. Gonzalez-Guzman et al. Plant Cell. (212). 1.115/tpc.112.98574 Supplemental Figure 1. Gene expression levels of the PYR/PYL/RCAR ABA receptors in the Arabidopsis transcriptome genomic
More informationSUPPLEMENTARY INFORMATION
Supplemental Methods Isolation and mapping of SPCH An EMS-mutagenized population of tmm-1 (Col);E1728 (an enhancer trap GFP marking guard cells) was created. M2 seeds were collected from M1 s planted in
More informationSupplemental material
Supplemental material Table 1- Segregation analysis of sgt1a sgt1b double mutant plants Parental genotype No. of lines Normal seeds Phenotype Abnormal seeds Ratio of abnormal seeds (%) WT 3 171 2 1.16
More informationcote cote-yfp spc This work cote cote-yfp spc This work cote cote-yfp spc This work cote cote-yfp spc This work cote cote-yfp spc This work
SUPPLEMENTARY INFORMATION Table S1. List of strains Strains Genotype Source B. subtilis PY79 Prototrophic derivative of B. subtilis 168 (60) RL1070 spovid::kan (12) HS176 cotz phs2 (cotz-gfp spc) (22)
More informationEXPRESSION OF THE FIS2 PROMOTER IN ARABIDOPSIS THALIANA
EXPRESSION OF THE FIS2 PROMOTER IN ARABIDOPSIS THALIANA Item Type text; Electronic Thesis Authors Bergstrand, Lauren Janel Publisher The University of Arizona. Rights Copyright is held by the author. Digital
More informationJMJ14-HA. Col. Col. jmj14-1. jmj14-1 JMJ14ΔFYR-HA. Methylene Blue. Methylene Blue
Fig. S1 JMJ14 JMJ14 JMJ14ΔFYR Methylene Blue Col jmj14-1 JMJ14-HA Methylene Blue Col jmj14-1 JMJ14ΔFYR-HA Fig. S1. The expression level of JMJ14 and truncated JMJ14 with FYR (FYRN + FYRC) domain deletion
More informationSupplementary Figure 1. Nature Genetics: doi: /ng.3848
Supplementary Figure 1 Phenotypes and epigenetic properties of Fab2L flies. A- Phenotypic classification based on eye pigment levels in Fab2L male (orange bars) and female (yellow bars) flies (n>150).
More informationGFP WxTP-GFP WxTP-GFP. stromules. DsRed WxTP-DsRed WxTP-DsRed. stromules
Supplemental Data. Kitajima et al. (2009). The rice -amylase glycoprotein is targeted from the Golgi apparatus through the secretory pathway to the plastids. A GFP WxTP-GFP WxTP-GFP stromules stromules
More informationHeterosis and inbreeding depression of epigenetic Arabidopsis hybrids
Heterosis and inbreeding depression of epigenetic Arabidopsis hybrids Plant growth conditions The soil was a 1:1 v/v mixture of loamy soil and organic compost. Initial soil water content was determined
More informationSupplementary Figure 1. Real time in vivo imaging of SG secretion. (a) SGs from Drosophila third instar larvae that express Sgs3-GFP (green) and
Supplementary Figure 1. Real time in vivo imaging of SG secretion. (a) SGs from Drosophila third instar larvae that express Sgs3-GFP (green) and Lifeact-Ruby (red) were imaged in vivo to visualize secretion
More informationLipid transfer proteins confer resistance to trichothecenes
Lipid transfer proteins confer resistance to trichothecenes John McLaughlin and Anwar Bin-Umer Tumer Laboratory National Fusarium Head Blight Forum December 6th, 2012 FY09-11: Identify trichothecene resistance
More informationSupplemental Data. Lermontova et al. (2013). Plant Cell /tpc
Supplemental Figure 1. Multiple alignment of the N-terminal parts of plant KNL2 orthologs. Multiple sequence alignment was performed by the MUSCLE method and visualized by the JalView program. Highly conserved
More informationSupplemental Data. Tameling et al. (2010). Plant Cell 10.1105/tpc.110.077461 Supplemental Table 1. Primers used in plasmid construction Primer Code Sequence eo1 5 -GCCATGGCTCATGCAAGTGTGGCTTCTC-3 eo2 5
More information. Supplementary Information
. Supplementary Information Supplementary Figure S1. Mature embryo sac observations. Supplementary Figure S2. STT observations. Supplementary Figure S3. Comparison of the PTB1 cdna with that of the mutant.
More informationPhotoreceptor Regulation of Constans Protein in Photoperiodic Flowering
Photoreceptor Regulation of Constans Protein in Photoperiodic Flowering by Valverde et. Al Published in Science 2004 Presented by Boyana Grigorova CBMG 688R Feb. 12, 2007 Circadian Rhythms: The Clock Within
More informationLife in an unusual intracellular niche a bacterial symbiont infecting the nucleus of amoebae
Life in an unusual intracellular niche a bacterial symbiont infecting the nucleus of amoebae Frederik Schulz, Ilias Lagkouvardos, Florian Wascher, Karin Aistleitner, Rok Kostanjšek, Matthias Horn Supplementary
More informationNature Genetics: doi: /ng Supplementary Figure 1. The FIN and FAB genes act separately from the meristem maturation pathway.
Supplementary Figure 1 The FIN and FAB genes act separately from the meristem maturation pathway. (a) Representative inflorescence from the compound inflorescence (s, defective in the homolog of Arabidopsis
More informationSupp- Figure 2 Confocal micrograph of N. benthamiana tissues transiently expressing 35S:YFP-PDCB1. PDCB1 was targeted to plasmodesmata (twin punctate
Supplemental Data. Simpson et al. (009). n rabidopsis GPI-anchor plasmodesmal neck protein with callosebinding activity and potential to regulate cell-to-cell trafficking. 5 0 stack Supp- Figure Confocal
More informationSupporting Online Material
1 Stomatal Patterning and Differentiation by Synergistic Interactions of Receptor Kinases Elena D. Shpak, Jessica Messmer McAbee, Lynn Jo Pillitteri, and Keiko U. Torii Supporting Online Material Material
More informationSupplemental Figure 1. Comparison of Tiller Bud Formation between the Wild Type and d27. (A) and (B) Longitudinal sections of shoot apex in wild-type
A B 2 3 3 2 1 1 Supplemental Figure 1. Comparison of Tiller Bud Formation between the Wild Type and d27. (A) and (B) Longitudinal sections of shoot apex in wild-type (A) and d27 (B) seedlings at the four
More informationSUPPLEMENTAL MATERIAL
SUPPLEMENTAL MATERIAL Figure S1. Mitochondrial morphology in Fis1-null, Mff-null and Fis1/Mff-null MEF cells. (A) Western blotting of lysates from Fis1-null, Mff-null and Fis1/Mff-null cells. Lysates were
More informationTable S1. Primers used for the constructions of recombinant GAL1 and λ5 mutants. GAL1-E74A ccgagcagcgggcggctgtctttcc ggaaagacagccgcccgctgctcgg
SUPPLEMENTAL DATA Table S1. Primers used for the constructions of recombinant GAL1 and λ5 mutants Sense primer (5 to 3 ) Anti-sense primer (5 to 3 ) GAL1 mutants GAL1-E74A ccgagcagcgggcggctgtctttcc ggaaagacagccgcccgctgctcgg
More informationSUPPLEMENTARY INFORMATION
doi:1.138/nature111 cytosol Model: PILS function in cellular auxin homeostasis ER nucleus IAA degradation? sequestration? conjugation? storage? signalling? PILS IAA ER cytosol Supplemental Figure 1 Model
More informationArabidopsis COMPASS-Like Complexes Mediate Histone H3 Lysine-4 Trimethylation to Control Floral Transition and Plant Development
Arabidopsis COMPASS-Like Complexes Mediate Histone H3 Lysine-4 Trimethylation to Control Floral Transition and Plant Development Danhua Jiang 1,2, Nicholas C. Kong 1,2, Xiaofeng Gu 2, Zicong Li 1, Yuehui
More informationSupplementary Figure 1. Nature Biotechnology: doi: /nbt.4067
Supplementary Figure 1 Phylogenetic tree of GAUT Protein Family and gene model, RNAi construct, and relative transcript abundance of GAUT4 in switchgrass, rice and poplar knockdown (KD) lines. Phylogenetic
More informationSupplemental material
Supplemental material THE JOURNAL OF CELL BIOLOGY Mourier et al., http://www.jcb.org/cgi/content/full/jcb.201411100/dc1 Figure S1. Size and mitochondrial content in Mfn1 and Mfn2 knockout hearts. (A) Body
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:10.1038/nature11524 Supplementary discussion Functional analysis of the sugar porter family (SP) signature motifs. As seen in Fig. 5c, single point mutation of the conserved
More informationconcentration ( mol l -1 )
concentration ( mol l -1 ) 8 10 0 20 40 60 80 100 120 140 160 180 methane sulfide ammonium oxygen sulfate (/10) b depth (m) 12 14 Supplementary Figure 1. Water column parameters from August 2011. Chemical
More informationSUPPLEMENTARY INFORMATION
Supplementary Figure 1 Sns and Duf co-localise in embryonic nephrocytes a-c, Wild-type stage 11 (a),14 (b) and 16 (c) embryos stained with anti-duf (green) and anti-sns (red). Higher magnification images
More informationSupplementary Information for. Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria
Supplementary Information for Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria Isabella Santi 1 *, Neeraj Dhar 1, Djenet Bousbaine 1, Yuichi Wakamoto, John D.
More informationMaria V. Yamburenko, Yan O. Zubo, Radomíra Vanková, Victor V. Kusnetsov, Olga N. Kulaeva, Thomas Börner
ABA represses the transcription of chloroplast genes Maria V. Yamburenko, Yan O. Zubo, Radomíra Vanková, Victor V. Kusnetsov, Olga N. Kulaeva, Thomas Börner Supplementary data Supplementary tables Table
More informationGenetic interaction and phenotypic analysis of the Arabidopsis MAP kinase pathway mutations mekk1 and mpk4 suggests signaling pathway complexity
Genetic interaction and phenotypic analysis of the Arabidopsis MAP kinase pathway mutations mekk1 and mpk4 suggests signaling pathway complexity Shih-Heng Su, Maria Cristina Suarez-Rodriguez, Patrick Krysan
More informationIllegitimate translation causes unexpected gene expression from on-target out-of-frame alleles
Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9 Shigeru Makino, Ryutaro Fukumura, Yoichi Gondo* Mutagenesis and Genomics Team, RIKEN
More informationPostdoc Fellowships for non-eu researchers. Final Report
Postdoc Fellowships for non-eu researchers Final Report Name Andrés Ritter Selection 2011 Host institution Gent University Supervisor Professor Dirk Inzé and Professor Alain Goossens Period covered by
More informationDIA-Umpire: comprehensive computational framework for data independent acquisition proteomics
DIA-Umpire: comprehensive computational framework for data independent acquisition proteomics Chih-Chiang Tsou 1,2, Dmitry Avtonomov 2, Brett Larsen 3, Monika Tucholska 3, Hyungwon Choi 4 Anne-Claude Gingras
More informationSUPPLEMENTARY INFORMATION
doi:10.1038/nature11589 Supplementary Figure 1 Ciona intestinalis and Petromyzon marinus neural crest expression domain comparison. Cartoon shows dorsal views of Ciona mid gastrula (left) and Petromyzon
More informationCharacterisation of abiotic stress inducible plant promoters and bacterial genes for osmotolerance using transgenic approach
Characterisation of abiotic stress inducible plant promoters and bacterial genes for osmotolerance using transgenic approach ABSTRACT SUBMITTED TO JAMIA MILLIA ISLAMIA NEW DELHI IN PARTIAL FULFILMENT OF
More informationTransitivity-dependent and transitivity-independent cell-to-cell movement of RNA
Himber et al. Transitivity-dependent and transitivity-independent cell-to-cell movement of RNA silencing SUPPLEMENTAL MATERIAL Supplemental material 1. Short-range movement of GFP silencing affects a nearly
More informationSupporting Information
Supporting Information Cao et al. 10.1073/pnas.1306220110 Gram - bacteria Hemolymph Cytoplasm PGRP-LC TAK1 signalosome Imd dfadd Dredd Dnr1 Ikk signalosome P Relish Nucleus AMP and effector genes Fig.
More informationFigure S1. Programmed cell death in the AB lineage occurs in temporally distinct
SUPPLEMENTAL FIGURE LEGENDS Figure S1. Programmed cell death in the AB lineage occurs in temporally distinct waves. (A) A representative sub-lineage (ABala) of the C. elegans lineage tree (adapted from
More informationGenomics and bioinformatics summary. Finding genes -- computer searches
Genomics and bioinformatics summary 1. Gene finding: computer searches, cdnas, ESTs, 2. Microarrays 3. Use BLAST to find homologous sequences 4. Multiple sequence alignments (MSAs) 5. Trees quantify sequence
More informationModel plants and their Role in genetic manipulation. Mitesh Shrestha
Model plants and their Role in genetic manipulation Mitesh Shrestha Definition of Model Organism Specific species or organism Extensively studied in research laboratories Advance our understanding of Cellular
More informationBiological Roles of Cytokinins
Direct Control of Shoot Meristem Activity by a Cytokinin-Activating Enzyme By Kurakawa et. Al. Published in Nature Presented by Boyana Grigorova Biological Roles of Cytokinins Cytokinins are positive regulators
More informationTCP Transcription Factors Link the Regulation of Genes Encoding Mitochondrial Proteins with the Circadian Clock in Arabidopsis thaliana W OA
This article is a Plant Cell Advance Online Publication. The date of its first appearance online is the official date of publication. The article has been edited and the authors have corrected proofs,
More informationSupporting Information
Supporting Information Mullins et al. 10.1073/pnas.0906781106 SI Text Detection of Calcium Binding by 45 Ca 2 Overlay. The 45 CaCl 2 (1 mci, 37 MBq) was obtained from NEN. The general method of 45 Ca 2
More informationMeasuring TF-DNA interactions
Measuring TF-DNA interactions How is Biological Complexity Achieved? Mediated by Transcription Factors (TFs) 2 Regulation of Gene Expression by Transcription Factors TF trans-acting factors TF TF TF TF
More informationSchool of Life Sciences, Tsinghua University, Beijing , China b. College of Animal Sciences, Zhejiang University, Hangzhou , China
The Plant Cell, Vol. 23: 1000 1013, March 2011, www.plantcell.org ã 2011 American Society of Plant Biologists The Jasmonate-ZIM Domain Proteins Interact with the R2R3-MYB Transcription Factors MYB21 and
More informationBy Jonathan I. Watkinson. Virginia Polytechnic Institute and State University. Doctor of Philosophy Horticulture
Characterization of two genes, trehalose-6-phosphate synthase/phosphatase and nucleotide binding protein, shown to be differentially regulated in roots of Cypripedium parviflorum var. pubescens grown with
More informationGENETIC ANALYSES OF ROOT SYSTEM DEVELOPMENT IN THE TOMATO CROP MODEL
GENETIC ANALYSES OF ROOT SYSTEM DEVELOPMENT IN THE TOMATO CROP MODEL Kelsey Hoth 1 Dr. Maria Ivanchenko 2 Bioresourse Research 1, Department of Botany and Plant Physiology 2, Oregon State University, Corvallis,
More informationHeterozygous BMN lines
Optical density at 80 hours 0.8 0.6 0.4 0.2 0.8 0.6 0.4 0.2 0.8 0.6 0.4 0.2 0.8 0.6 0.4 0.2 a YPD b YPD + 1µM nystatin c YPD + 2µM nystatin d YPD + 4µM nystatin 1 3 5 6 9 13 16 20 21 22 23 25 28 29 30
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2647 Figure S1 Other Rab GTPases do not co-localize with the ER. a, Cos-7 cells cotransfected with an ER luminal marker (either KDEL-venus or mch-kdel) and mch-tagged human Rab5 (mch-rab5,
More informationBLAST. Varieties of BLAST
BLAST Basic Local Alignment Search Tool (1990) Altschul, Gish, Miller, Myers, & Lipman Uses short-cuts or heuristics to improve search speed Like speed-reading, does not examine every nucleotide of database
More informationSupplementary Information
Supplementary Information Rice APC/C TE controls tillering through mediating the degradation of MONOCULM 1 Qibing Lin 1*, Dan Wang 1*, Hui Dong 2*, Suhai Gu 1, Zhijun Cheng 1, Jie Gong 2, Ruizhen Qin 1,
More informationSupporting online material
Supporting online material Materials and Methods Target proteins All predicted ORFs in the E. coli genome (1) were downloaded from the Colibri data base (2) (http://genolist.pasteur.fr/colibri/). 737 proteins
More informationHRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds
HRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds Chongming Wu 1,2., Juanjuan Feng 1,2., Ran Wang 1,2, Hong Liu 1,2, Huixia Yang 1,2, Pedro
More informationSUPPLEMENTARY INFORMATION
med!1,2 Wild-type (N2) end!3 elt!2 5 1 15 Time (minutes) 5 1 15 Time (minutes) med!1,2 end!3 5 1 15 Time (minutes) elt!2 5 1 15 Time (minutes) Supplementary Figure 1: Number of med-1,2, end-3, end-1 and
More informationSupplementary Figure 3
Supplementary Figure 3 7.0 Col Kas-1 Line FTH1A 8.4 F3PII3 8.9 F26H11 ATQ1 T9I22 PLS8 F26B6-B 9.6 F27L4 9.81 F27D4 9.92 9.96 10.12 10.14 10.2 11.1 0.5 Mb T1D16 Col % RGR 83.3 101 227 93.5 75.9 132 90 375
More informationSupplementary Methods
Supplementary Methods Microarray analysis Grains of 7 DAP of the wild-type and gif1 were harvested for RNA preparation. Microarray analysis was performed with the Affymetrix (Santa Clara, CA) GeneChip
More informationSupplemental Figures. Supplemental Data. Sugliani et al. Plant Cell (2016) /tpc Clades RSH1. Rsh1[HS] RSH2 RSH3. Rsh4[HS] HYD TGS ACT
Supplemental Figures Clades RSH1 TP - HYD TGS ACT Rsh1[HS] RSH2 TP HYD SYN TGS Rsh2[HS] RSH3 TP HYD SYN TGS CRSH TP - SYN EFh Rsh4[HS] Supplemental Figure 1. Arabidopsis RSH domain structure. Schematic
More informationRegulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings
The Plant Journal (2008) 53, 814 827 doi: 10.1111/j.1365-313X.2007.03373.x Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings Antonio
More informationSupplementary Figure 1: To test the role of mir-17~92 in orthologous genetic model of ADPKD, we generated Ksp/Cre;Pkd1 F/F (Pkd1-KO) and Ksp/Cre;Pkd1
Supplementary Figure 1: To test the role of mir-17~92 in orthologous genetic model of ADPKD, we generated Ksp/Cre;Pkd1 F/F (Pkd1-KO) and Ksp/Cre;Pkd1 F/F ;mir-17~92 F/F (Pkd1-miR-17~92KO) mice. (A) Q-PCR
More informationEvolution, Expression Differentiation and Interaction Specificity of Heterotrimeric G-Protein Subunit Gene Family in the Mesohexaploid Brassica rapa
Evolution, Expression Differentiation and Interaction Specificity of Heterotrimeric G-Protein Subunit Gene Family in the Mesohexaploid Brassica rapa Gulab C. Arya, Roshan Kumar, Naveen C. Bisht* National
More informationSupplementary Materials for
advances.sciencemag.org/cgi/content/full/4/5/eaar2740/dc1 Supplementary Materials for The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres
More informationComparative Protein Modeling of Superoxide Dismutase Isoforms in Maize.
Comparative Protein Modeling of Superoxide Dismutase Isoforms in Maize. Kaliyugam Shiriga 1, 2, Rinku Sharma 1, Krishan Kumar 2, Firoz Hossain 1 and NepoleanThirunavukkarasu 1* Division of Genetics, Indian
More informationThree Myosins Contribute Uniquely to the Assembly and Constriction of the Fission Yeast Cytokinetic Contractile Ring
Current Biology Supplemental Information Three Myosins Contribute Uniquely to the Assembly and Constriction of the Fission Yeast Cytokinetic Contractile Ring Caroline Laplante, Julien Berro, Erdem Karatekin,
More information