Lipid transfer proteins confer resistance to trichothecenes
|
|
- Lindsey Holt
- 6 years ago
- Views:
Transcription
1 Lipid transfer proteins confer resistance to trichothecenes John McLaughlin and Anwar Bin-Umer Tumer Laboratory National Fusarium Head Blight Forum December 6th, 2012
2 FY09-11: Identify trichothecene resistance genes by genomewide screening in Saccharomyces cerevisiae Translation, mito morphology, lipid metabolism FY11-13 Identify plant genes for trichothecene resistance by activation tagging in Arabidopsis thaliana FY13-14 Demonstrate resistance in wheat 2
3 Screening Arabidopsis (Col-0) for Tcin sensitivity using a germination assay (14 dpg) 0 µm Tcin 1 µm Tcin 2 µm Tcin 3 µm Tcin
4 Root/vegetative growth is severely inhibited at Tcin concentrations greater than 1 µm 0 µm 1 µm 2 µm 3 µm
5 Activation tagging: Dominant Gain-of-function promoter transcription pattern ORF Susceptible to toxin Insertion of activation tagged vector containing 4X enhancers enhancers promoter new transcription pattern ORF Resistance to toxin
6 Activation Tagging in Arabidopsis Transformation of Col-O with pski015 (floral dip) Generate M1 and M2 populations TAIL PCR To Identify Flanking Sequence RNA isolation and qrt PCR to measure gene expression?? Overexpression Detected 1º Screen Identify M2 plants which set root Test independently generated knockout (SALK) Transform Col-0 With overexpression gene vector 2º Screen Confirmation of Phenotype Specific controlling gene Introduce gene into crop (or knockout) 6
7 250,000 activation tagged lines were screened Chr. 5 At5G55430 Unknown At5G55440 (AtTRRF1) Unknown Ltp4.4 Ltp4.5 CaMV 35S enhancers LB 1k b
8 Non-specific Lipid Transfer Proteins (nsltps) Plant nsltps are small cysteine-rich lipid-binding proteins Key role in formation of cuticular wax layers Role in plant resistance to biotic and abiotic stress Upregulated during F. graminearum infection of wheat & barley. A wheat nsltp gene expression is correlated with 5A QTL associated with type I resistance to FHB
9 Plant nsltp functions LTPs bind to and transfer phospholipids between membranes in vitro Plant nsltps contain an internal hydrophobic pocket, which can accommodate a lipid Plant nsltps contain signal peptides, which target them to cell wall/apoplast Some nsltps are upregulated in response to infection and exhibit antibacterial and antifungal activity
10 LTP4.4 LTP4.5 LTP4.1 Predicted Structures for LTP4.4 and LTP4.5 compared to the X-ray crystal structure of DIR1 (Defective in induced resistance) from Arabidopsis (Type 4.1 LTP) bound to a lipid
11 A.t. Type IV nsltps: LTP4.1 (DIR1) In Arabidopsis 49 nsltps are grouped into nine types based on sequence homology. LTP4.2 LTP4.3 LTP4.4 LTP4.5 Boutrot, F. et al. BMC Genomics. 9:86 (2008)
12 LTP4.4:GFP is localized in the cell wall 20 µm
13 LTP4.5:GFP Localization in Arabidopsis
14 LTP4.4:GFP is expressed in the roots Col-0 LTP4.4 (Line 13)
15 LTP4.4 protein expression in Arabidopsis LTP4:GFP SAT1 Immunoblot Analysis of transgenic Arabidopsis lines expressing LTP4.4:GFP.
16 Transgenic Arabidopsis overexpressing LTP4.4 Col-0 LTP LTP µm Tcin 0 µm Tcin Transgenic Arabidopsis overexpressing LTP4.4 are phenotypically normal and show resistance to Tcin
17 Transgenic Arabidopsis lines overexpressing LTP4.4 and LTP4.5 are able to form roots on media containing 4 μm Tcin better than wild type control plants
18 Conclusions Activation tagging in Arabidopsis identified a trichothecin resistant mutant containing a modified T-DNA vector, which enhanced expression of two nonspecific lipid transfer protein (nsltp) genes. Overexpression of each nsltp gene in Arabidopsis provided resistance to trichothecin, demonstrating that resistance in the T-DNA tagged line was due to overexpression of the nsltp genes LTP4.4 and LTP4.5 localize to the cell wall/apoplast in the mesophyll, the epidermis, and root tissues.
19 Ongoing and future work Determine if expression of LTP4.4 and LTP4.5 confers resistance to DON and FHB in transgenic wheat and barley Lipid peroxidation is associated with trichothecene toxicity- Connection between LTPs and lipid damage? LTP genes may function in the production of a mobile signal for resistance, affect lipid composition of membranes/cuticle, or they may bind to trichothecenes Isolate recombinant LTP4.4 and LTP4.5 proteins using the Pichia system Binding of LTPs to trichothecenes using Surface plasmon resonance (SPR) analysis
20 Characterizing LTPs in yeast Can At. LTP4.4 & At. LTP4.5 provide resistance to Tcin in yeast?
21 OD600 OD600 LTP expression leads to Tcin resistance in yeast Cloned LTPs into a yeast expression system and transformed them into yeast. Grew yeast in selective media +/- Tcin Growth at 3µM Tcin VC LTP4.4 LTP Hours Post Treatment Growth at 4µM Tcin VC LTP4.4 LTP Hours Post Treatment
22 Is LTP-mediated resistance a general response?
23 OD600 LTP-mediated resistance is specific to Tcin Inhibitors of yeast growth & translation: Cycloheximide Growth at 0.25µg/mL Cycloheximide VC LTP4.4 LTP Hours Post Treatment
24 OD600 LTP-mediated resistance is specific to Tcin Inhibitors of yeast growth & translation: Anisomycin Growth at 7.5µg/mL Anisomycin VC LTP4.4 LTP Hours Post Treatment
25 OD600 LTP-mediated resistance is specific to Tcin Inhibitors of yeast growth & translation: Chloramphenicol Growth at 5mg/mL Chloramphenicol VC LTP4.4 LTP Hours Post Treatment
26 Do all LTPs provide resistance to trichothecenes?
27 OD600 OD600 Not all LTPs mediate resistance to trichothecenes At. LTP1.1 done not confer resistance but rather increases susceptibility Growth at 3µM Tcin VC LTP Hours Post Treatment Growth at 4µM Tcin VC LTP Hours Post Treatment
28 Are early and late time point events due to Tcin affected by LTP overexpression?
29 ROS generating cells LTP overexpression lowers ROS levels Reactive oxygen species (ROS) levels were detected by DCFH-DA staining and quantified by flow cytometry 10% 8% 6% VC LTP4 LTP5 4% 2% 0% [Tcin] µm
30 Tcin Treatment Untreated Tcin causes mitochondrial fragmentation in yeast DIC GFP Wild type cells: Tubular network 6h post treatment fragmented mitochondria
31 GFP DIC LTP overexpression minimize mitochondrial fragmentation VC LTP4.4 LTP4.5
32 Is inhibition of total & mitochondrial translation affected by LTP overexpression?
33 Total TL% Relative to Untreated Total translation is inhibited in LTP overexpressing cells µM 0.5µM 1µM 2µM VC-HA LTP4-HA LTP5-HA 4µM [Tcin]
34 Mito TL% Relative to Untreated Mitochondrial translation is not significantly inhibited in LTP overexpressing cells µM 0.5µM 1µM 2µM 4µM VC LTP4 LTP [Tcin] µm
35 To summarize Yeast serves as an ideal tool to identify plant LTPs that confer trichothecene resistance at the single cell level. At. LTPs participate directly in resistance to Tcin. LTP-mediated resistance is specific to trichothecenes and is not part of a general response against other translation inhibitors. Mitochondria plays a role in LTP-mediated resistance to Tcin.
36 ACKNOWLEDGMENTS Tumer Lab Dr. Susan McCormick Dr. Nilgun Tumer Dr. John McLaughlin Dr. Xiao-Ping Li Dr. Jennifer Nielson Kahn Dr. Mike Pierce Dr. Kerrie May Qing Yan Debaleena Basu Project Funding:
Characterisation of abiotic stress inducible plant promoters and bacterial genes for osmotolerance using transgenic approach
Characterisation of abiotic stress inducible plant promoters and bacterial genes for osmotolerance using transgenic approach ABSTRACT SUBMITTED TO JAMIA MILLIA ISLAMIA NEW DELHI IN PARTIAL FULFILMENT OF
More informationArabidopsis PPR40 connects abiotic stress responses to mitochondrial electron transport
Ph.D. thesis Arabidopsis PPR40 connects abiotic stress responses to mitochondrial electron transport Zsigmond Laura Supervisor: Dr. Szabados László Arabidopsis Molecular Genetic Group Institute of Plant
More information23-. Shoot and root development depend on ratio of IAA/CK
Balance of Hormones regulate growth and development Environmental factors regulate hormone levels light- e.g. phototropism gravity- e.g. gravitropism temperature Mode of action of each hormone 1. Signal
More informationRegulation of mycotoxin production and kinome analysis in Fusarium graminearum
Regulation of mycotoxin production and kinome analysis in Fusarium graminearum Chenfang Wang Northwest Agricultural & Forestry University Regulation of mycotoxin production and kinome analysis in Fusarium
More informationSupplemental Data. Chen and Thelen (2010). Plant Cell /tpc
Supplemental Data. Chen and Thelen (2010). Plant Cell 10.1105/tpc.109.071837 1 C Total 5 kg 20 kg 100 kg Transmission Image 100 kg soluble pdtpi-gfp Plastid (PDH-alpha) Mito (PDH-alpha) GFP Image vector
More informationNature Genetics: doi: /ng Supplementary Figure 1. The phenotypes of PI , BR121, and Harosoy under short-day conditions.
Supplementary Figure 1 The phenotypes of PI 159925, BR121, and Harosoy under short-day conditions. (a) Plant height. (b) Number of branches. (c) Average internode length. (d) Number of nodes. (e) Pods
More informationThe sucrose non-fermenting-1-related protein kinases SAPK1 and SAPK2 function collaboratively as positive regulators of salt stress tolerance in rice
Lou et al. BMC Plant Biology (2018) 18:203 https://doi.org/10.1186/s12870-018-1408-0 RESEARCH ARTICLE Open Access The sucrose non-fermenting-1-related protein kinases SAPK1 and SAPK2 function collaboratively
More informationGFP GAL bp 3964 bp
Supplemental Data. Møller et al. (2009) Shoot Na + exclusion and increased salinity tolerance engineered by cell type-specific alteration of Na + transport in Arabidopsis Supplemental Figure 1. Salt-sensitive
More informationLast time: Obtaining information from a cloned gene
Last time: Obtaining information from a cloned gene Objectives: 1. What is the biochemical role of the gene? 2. Where and when is the gene expressed (transcribed)? 3. Where and when is the protein made?
More informationLooking for LOV: Location of LOV1 function in Nicotiana benthamiana cells
Looking for LOV: Location of LOV1 function in Nicotiana benthamiana cells By: Patrick Rutledge 1 Dr. Jennifer Lorang 2,3, Dr. Marc Curtis 2,3, Dr. Thomas Wolpert 2,3 BioResource Research 1, Botany and
More informationSupplementary Figure 1
Supplementary Figure 1 Supplementary Figure 1. HSP21 expression in 35S:HSP21 and hsp21 knockdown plants. (a) Since no T- DNA insertion line for HSP21 is available in the publicly available T-DNA collections,
More informationThe geneticist s questions. Deleting yeast genes. Functional genomics. From Wikipedia, the free encyclopedia
From Wikipedia, the free encyclopedia Functional genomics..is a field of molecular biology that attempts to make use of the vast wealth of data produced by genomic projects (such as genome sequencing projects)
More information4) Please cite Dagda et al J Biol Chem 284: , for any publications or presentations resulting from use or modification of the macro.
Supplement Figure S1. Algorithmic quantification of mitochondrial morphology in SH- SY5Y cells treated with known fission/fusion mediators. Parental SH-SY5Y cells were transiently transfected with an empty
More informationSupp- Figure 2 Confocal micrograph of N. benthamiana tissues transiently expressing 35S:YFP-PDCB1. PDCB1 was targeted to plasmodesmata (twin punctate
Supplemental Data. Simpson et al. (009). n rabidopsis GPI-anchor plasmodesmal neck protein with callosebinding activity and potential to regulate cell-to-cell trafficking. 5 0 stack Supp- Figure Confocal
More informationLife Science Journal 2014;11(9) Cryptochrome 2 negatively regulates ABA-dependent seed germination in Arabidopsis
Cryptochrome 2 negatively regulates ABA-dependent seed germination in Arabidopsis Sung-Il Kim 1, Sang Ik Song 3, Hak Soo Seo 1, 2, 4 * 1 Department of Plant Science and Research Institute of Agriculture
More informationSUPPLEMENTARY INFORMATION
Supplemental Methods Isolation and mapping of SPCH An EMS-mutagenized population of tmm-1 (Col);E1728 (an enhancer trap GFP marking guard cells) was created. M2 seeds were collected from M1 s planted in
More informationTrichothecene Mycotoxins Inhibit Mitochondrial Translation Implication for the Mechanism of Toxicity
Toxins 2011, 3, 1484-1501; doi:10.3390/toxins3121484 Article OPEN ACCESS toxins ISSN 2072-6651 www.mdpi.com/journal/toxins Trichothecene Mycotoxins Inhibit Mitochondrial Translation Implication for the
More informationSupplemental Data. Perrella et al. (2013). Plant Cell /tpc
Intensity Intensity Intensity Intensity Intensity Intensity 150 50 150 0 10 20 50 C 150 0 10 20 50 D 0 10 20 Distance (μm) 50 20 40 E 50 F 0 10 20 50 0 15 30 Distance (μm) Supplemental Figure 1: Co-localization
More informationFigure 1. Identification of UGT74E2 as an IBA glycosyltransferase. (A) Relative conversion rates of different plant hormones to their glucosylated
Figure 1. Identification of UGT74E2 as an IBA glycosyltransferase. (A) Relative conversion rates of different plant hormones to their glucosylated form by recombinant UGT74E2. The naturally occurring auxin
More informationSUPPLEMENTARY INFORMATION
reverse 3175 3175 F L C 318 318 3185 3185 319 319 3195 3195 315 8 1 315 3155 315 317 Supplementary Figure 3. Stability of expression of the GFP sensor constructs return to warm conditions. Semi-quantitative
More informationSupplemental Data. Wang et al. (2014). Plant Cell /tpc
Supplemental Figure1: Mock and NPA-treated tomato plants. (A) NPA treated tomato (cv. Moneymaker) developed a pin-like inflorescence (arrowhead). (B) Comparison of first and second leaves from mock and
More information** LCA LCN PCA
% of wild type value % of wild type value a 12 1 8 2 b 12 1 8 2 LCA LCN PCA Col- sod3-1 Supplementary Figure 1 sod3-1 influences cell proliferation. (a) Fifth leaf cell area (LCA) and leaf cell number
More informationBiology 112 Practice Midterm Questions
Biology 112 Practice Midterm Questions 1. Identify which statement is true or false I. Bacterial cell walls prevent osmotic lysis II. All bacterial cell walls contain an LPS layer III. In a Gram stain,
More informationCHARACTERIZING THE ROLE OF MITOCHONDRIA IN THE TOXICITY OF TRICHOTHECENES PRODUCED BY FUSARIUM GRAMINEARUM MOHAMED ANWAR BIN UMER
CHARACTERIZING THE ROLE OF MITOCHONDRIA IN THE TOXICITY OF TRICHOTHECENES PRODUCED BY FUSARIUM GRAMINEARUM by MOHAMED ANWAR BIN UMER A Dissertation submitted to the Graduate School-New Brunswick Rutgers,
More informationGenetics 275 Notes Week 7
Cytoplasmic Inheritance Genetics 275 Notes Week 7 Criteriafor recognition of cytoplasmic inheritance: 1. Reciprocal crosses give different results -mainly due to the fact that the female parent contributes
More informationPlant mitochondrial dynamics
Plant mitochondrial dynamics Peroxisome Chloroplast Nucleus Mitochondria from Alberts et al. 1994 Living Arabidopsis leaf 10 µm Logan & Leaver (2000) Journal of Experimental Botany, 51: 865-871 5 µm S.
More informationRole of Mitochondrial Remodeling in Programmed Cell Death in
Developmental Cell, Vol. 12 Supplementary Data Role of Mitochondrial Remodeling in Programmed Cell Death in Drosophila melanogaster Gaurav Goyal, Brennan Fell, Apurva Sarin, Richard J. Youle, V. Sriram.
More informationCellular Neuroanatomy I The Prototypical Neuron: Soma. Reading: BCP Chapter 2
Cellular Neuroanatomy I The Prototypical Neuron: Soma Reading: BCP Chapter 2 Functional Unit of the Nervous System The functional unit of the nervous system is the neuron. Neurons are cells specialized
More information7.06 Problem Set #4, Spring 2005
7.06 Problem Set #4, Spring 2005 1. You re doing a mutant hunt in S. cerevisiae (budding yeast), looking for temperaturesensitive mutants that are defective in the cell cycle. You discover a mutant strain
More informationTi plasmid derived plant vector systems: binary and co - integrative vectors transformation process; regeneration of the transformed lines
Ti plasmid derived plant vector systems: binary and co - integrative vectors transformation process; regeneration of the transformed lines Mitesh Shrestha Constraints of Wild type Ti/Ri-plasmid Very large
More informationPrinciples of QTL Mapping. M.Imtiaz
Principles of QTL Mapping M.Imtiaz Introduction Definitions of terminology Reasons for QTL mapping Principles of QTL mapping Requirements For QTL Mapping Demonstration with experimental data Merit of QTL
More informationSupplemental Table 1. Primers used for cloning and PCR amplification in this study
Supplemental Table 1. Primers used for cloning and PCR amplification in this study Target Gene Primer sequence NATA1 (At2g393) forward GGG GAC AAG TTT GTA CAA AAA AGC AGG CTT CAT GGC GCC TCC AAC CGC AGC
More informationTransgenic plants over-expressing HbCuZnSOD cytosolic isoform are more tolerant to a water deficit
Transgenic plants over-expressing HbCuZnSOD cytosolic isoform are more tolerant to a water deficit J. Leclercq, F. Martin, C. Sanier, A. Clément-Vidal, D. Fabre, G. Oliver, L. Lardet, A. Ayar, M. Peyramard
More informationGene expression in prokaryotic and eukaryotic cells, Plasmids: types, maintenance and functions. Mitesh Shrestha
Gene expression in prokaryotic and eukaryotic cells, Plasmids: types, maintenance and functions. Mitesh Shrestha Plasmids 1. Extrachromosomal DNA, usually circular-parasite 2. Usually encode ancillary
More informationSupporting Online Material for
www.sciencemag.org/cgi/content/full/1121356/dc1 Supporting Online Material for Polar PIN Localization Directs Auxin Flow in Plants Justyna Wiśniewska, Jian Xu, Daniela Seifertová, Philip B. Brewer, Kamil
More informationSupplemental material
Supplemental material Table 1- Segregation analysis of sgt1a sgt1b double mutant plants Parental genotype No. of lines Normal seeds Phenotype Abnormal seeds Ratio of abnormal seeds (%) WT 3 171 2 1.16
More informationTHE ROLE OF THE PHYTOCHROME B PHOTORECEPTOR IN THE REGULATION OF PHOTOPERIODIC FLOWERING. AnitaHajdu. Thesis of the Ph.D.
THE ROLE OF THE PHYTOCHROME B PHOTORECEPTOR IN THE REGULATION OF PHOTOPERIODIC FLOWERING AnitaHajdu Thesis of the Ph.D. dissertation Supervisor: Dr. LászlóKozma-Bognár - senior research associate Doctoral
More informationA complementation test would be done by crossing the haploid strains and scoring the phenotype in the diploids.
Problem set H answers 1. To study DNA repair mechanisms, geneticists isolated yeast mutants that were sensitive to various types of radiation; for example, mutants that were more sensitive to UV light.
More informationMitochondria Mitochondria were first seen by kollicker in 1850 in muscles and called them sarcosomes. Flemming (1882) described these organelles as
Mitochondria Mitochondria were first seen by kollicker in 1850 in muscles and called them sarcosomes. Flemming (1882) described these organelles as filia Altmann (1890) observed these structures and named
More informationSupplementary Information
Supplementary Information MAP2/Hoechst Hyp.-AP ph 6.5 Hyp.-SD ph 7.2 Norm.-SD ph 7.2 Supplementary Figure 1. Mitochondrial elongation in cortical neurons by acidosis. Representative images of neuronal
More informationOptimization of Immunoblot Protocol for Use with a Yeast Strain Containing the CDC7 Gene Tagged with myc
OPTIMIZATION OF IMMUNOBLOT PROTOCOL 121 Optimization of Immunoblot Protocol for Use with a Yeast Strain Containing the CDC7 Gene Tagged with myc Jacqueline Bjornton and John Wheeler Faculty Sponsor: Anne
More informationDevelopment 143: doi: /dev : Supplementary information
Supplementary Materials and Methods Plant materials The mutants and transgenic plants used in the present study were as follows: E361 (from Alex Webb s laboratory); tmm-1, ptmm::tmm-gfp and flp-1 (from
More informationRegulation of Phosphate Homeostasis by microrna in Plants
Regulation of Phosphate Homeostasis by microrna in Plants Tzyy-Jen Chiou 1 *, Kyaw Aung 1,2, Shu-I Lin 1,3, Chia-Chune Wu 1, Su-Fen Chiang 1, and Chun-Lin Su 1 Abstract Upon phosphate (Pi) starvation,
More informationArabidopsis thaliana. Lucia Strader. Assistant Professor, Biology
Arabidopsis thaliana Lucia Strader Assistant Professor, Biology Arabidopsis as a genetic model Easy to grow Small genome Short life cycle Self fertile Produces many progeny Easily transformed HIV E. coli
More informationSOMNUS, a CCCH-Type Zinc Finger Protein in Arabidopsis, Negatively Regulates Light-Dependent Seed Germination Downstream of PIL5 W
The Plant Cell, Vol. 20: 1260 1277, May 2008, www.plantcell.org ª 2008 American Society of Plant Biologists SOMNUS, a CCCH-Type Zinc Finger Protein in Arabidopsis, Negatively Regulates Light-Dependent
More informationWelcome to BIOL 572: Recombinant DNA techniques
Lecture 1: 1 Welcome to BIOL 572: Recombinant DNA techniques Agenda 1: Introduce yourselves Agenda 2: Course introduction Agenda 3: Some logistics for BIOL 572 Agenda 4: Q&A section Agenda 1: Introduce
More informationLiu, Yang (2012) The characterization of a novel abscission-related gene in Arabidopsis thaliana. PhD thesis, University of Nottingham.
Liu, Yang (2012) The characterization of a novel abscission-related gene in Arabidopsis thaliana. PhD thesis, University of Nottingham. Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/12529/3/thesis_part_2_final.pdf
More informationEXPRESSION OF THE FIS2 PROMOTER IN ARABIDOPSIS THALIANA
EXPRESSION OF THE FIS2 PROMOTER IN ARABIDOPSIS THALIANA Item Type text; Electronic Thesis Authors Bergstrand, Lauren Janel Publisher The University of Arizona. Rights Copyright is held by the author. Digital
More informationSupplementary Methods
Supplementary Methods Microarray analysis Grains of 7 DAP of the wild-type and gif1 were harvested for RNA preparation. Microarray analysis was performed with the Affymetrix (Santa Clara, CA) GeneChip
More informationGSBHSRSBRSRRk IZTI/^Q. LlML. I Iv^O IV I I I FROM GENES TO GENOMES ^^^H*" ^^^^J*^ ill! BQPIP. illt. goidbkc. itip31. li4»twlil FIFTH EDITION
FIFTH EDITION IV I ^HHk ^ttm IZTI/^Q i I II MPHBBMWBBIHB '-llwmpbi^hbwm^^pfc ' GSBHSRSBRSRRk LlML I I \l 1MB ^HP'^^MMMP" jflp^^^^^^^^st I Iv^O FROM GENES TO GENOMES %^MiM^PM^^MWi99Mi$9i0^^ ^^^^^^^^^^^^^V^^^fii^^t^i^^^^^
More informationStress Effects on Myosin Mutant Root Length in Arabidopsis thaliana
University of Tennessee, Knoxville Trace: Tennessee Research and Creative Exchange University of Tennessee Honors Thesis Projects University of Tennessee Honors Program 5-2011 Stress Effects on Myosin
More informationFitness constraints on horizontal gene transfer
Fitness constraints on horizontal gene transfer Dan I Andersson University of Uppsala, Department of Medical Biochemistry and Microbiology, Uppsala, Sweden GMM 3, 30 Aug--2 Sep, Oslo, Norway Acknowledgements:
More informationMajor Plant Hormones 1.Auxins 2.Cytokinins 3.Gibberelins 4.Ethylene 5.Abscisic acid
Plant Hormones Lecture 9: Control Systems in Plants What is a Plant Hormone? Compound produced by one part of an organism that is translocated to other parts where it triggers a response in target cells
More informationSupporting online material
Supporting online material Materials and Methods Target proteins All predicted ORFs in the E. coli genome (1) were downloaded from the Colibri data base (2) (http://genolist.pasteur.fr/colibri/). 737 proteins
More informationM i t o c h o n d r i a
M i t o c h o n d r i a Dr. Diala Abu-Hassan School of Medicine dr.abuhassand@gmail.com Mitochondria Function: generation of metabolic energy in eukaryotic cells Generation of ATP from the breakdown of
More informationCommon Effects of Abiotic Stress Factors on Plants
Common Effects of Abiotic Stress Factors on Plants Plants are living organisms which lack ability of locomotion. Animals can move easily from one location to other. Immovable property of plants makes it
More informationTIME-LINE OF INFECTION
Review of Lecture 8: Getting inside the host is a critical step in disease development Fungal pathogens use contact and chemical tropisms to guide their way to a site where infection is possible Pathogens
More informationDOI: 10.1038/ncb2819 Gαi3 / Actin / Acetylated Tubulin Gαi3 / Actin / Acetylated Tubulin a a Gαi3 a Actin Gαi3 WT Gαi3 WT Gαi3 WT b b Gαi3 b Actin Gαi3 KO Gαi3 KO Gαi3 KO # # Figure S1 Loss of protein
More informationEpigenetics and Flowering Any potentially stable and heritable change in gene expression that occurs without a change in DNA sequence
Epigenetics and Flowering Any potentially stable and heritable change in gene expression that occurs without a change in DNA sequence www.plantcell.org/cgi/doi/10.1105/tpc.110.tt0110 Epigenetics Usually
More informationName: SBI 4U. Gene Expression Quiz. Overall Expectation:
Gene Expression Quiz Overall Expectation: - Demonstrate an understanding of concepts related to molecular genetics, and how genetic modification is applied in industry and agriculture Specific Expectation(s):
More informationGenetic dissection of the Arabidopsis thaliana ionome
Genetic dissection of the Arabidopsis thaliana ionome Genome Ionome Landscape distribution David E Salt Purdue University, USA What is the Ionome Environment Transcriptome Proteome Ionome The elemental
More informationAbiotic Stress in Crop Plants
1 Abiotic Stress in Crop Plants Mirza Hasanuzzaman, PhD Professor Department of Agronomy Sher-e-Bangla Agricultural University E-mail: mhzsauag@yahoo.com Stress Stress is usually defined as an external
More informationLecture 13: PROTEIN SYNTHESIS II- TRANSLATION
http://smtom.lecture.ub.ac.id/ Password: https://syukur16tom.wordpress.com/ Password: Lecture 13: PROTEIN SYNTHESIS II- TRANSLATION http://hyperphysics.phy-astr.gsu.edu/hbase/organic/imgorg/translation2.gif
More informationOutline. Leaf Development. Leaf Structure - Morphology. Leaf Structure - Morphology
Outline 1. Leaf Structure: Morphology & Anatomy 2. Leaf Development A. Anatomy B. Sector analysis C. Leaf Development Leaf Structure - Morphology Leaf Structure - Morphology 1 Leaf Structure - Morphology
More informationClimate Change and Plant Reproduction
Quantitative Trait Loci Mapping of Reproductive Traits Involved in Heat Stress Responses in Arabidopsis : Implications for Global Climate Change and Plant Reproduction Lazar Pavlovic, Greta Chiu, Jeffrey
More informationThe Research Plan. Functional Genomics Research Stream. Transcription Factors. Tuning In Is A Good Idea
Functional Genomics Research Stream The Research Plan Tuning In Is A Good Idea Research Meeting: March 23, 2010 The Road to Publication Transcription Factors Protein that binds specific DNA sequences controlling
More informationImportance of Protein sorting. A clue from plastid development
Importance of Protein sorting Cell organization depend on sorting proteins to their right destination. Cell functions depend on sorting proteins to their right destination. Examples: A. Energy production
More informationSupplementary Figure 1. Markedly decreased numbers of marginal zone B cells in DOCK8 mutant mice Supplementary Figure 2.
Supplementary Figure 1. Markedly decreased numbers of marginal zone B cells in DOCK8 mutant mice. Percentage of marginal zone B cells in the spleen of wild-type mice (+/+), mice homozygous for cpm or pri
More informationPlant Molecular and Cellular Biology Lecture 8: Mechanisms of Cell Cycle Control and DNA Synthesis Gary Peter
Plant Molecular and Cellular Biology Lecture 8: Mechanisms of Cell Cycle Control and DNA Synthesis Gary Peter 9/10/2008 1 Learning Objectives Explain why a cell cycle was selected for during evolution
More informationOld FINAL EXAM BIO409/509 NAME. Please number your answers and write them on the attached, lined paper.
Old FINAL EXAM BIO409/509 NAME Please number your answers and write them on the attached, lined paper. Gene expression can be regulated at several steps. Describe one example for each of the following:
More informationHRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds
HRS1 Acts as a Negative Regulator of Abscisic Acid Signaling to Promote Timely Germination of Arabidopsis Seeds Chongming Wu 1,2., Juanjuan Feng 1,2., Ran Wang 1,2, Hong Liu 1,2, Huixia Yang 1,2, Pedro
More informationSupplemental material
Supplemental material THE JOURNAL OF CELL BIOLOGY Mourier et al., http://www.jcb.org/cgi/content/full/jcb.201411100/dc1 Figure S1. Size and mitochondrial content in Mfn1 and Mfn2 knockout hearts. (A) Body
More informationGACE Biology Assessment Test I (026) Curriculum Crosswalk
Subarea I. Cell Biology: Cell Structure and Function (50%) Objective 1: Understands the basic biochemistry and metabolism of living organisms A. Understands the chemical structures and properties of biologically
More informationMicrobial Genetics, Mutation and Repair. 2. State the function of Rec A proteins in homologous genetic recombination.
Answer the following questions 1. Define genetic recombination. Microbial Genetics, Mutation and Repair 2. State the function of Rec A proteins in homologous genetic recombination. 3. List 3 types of bacterial
More informationI. Molecules & Cells. A. Unit One: The Nature of Science. B. Unit Two: The Chemistry of Life. C. Unit Three: The Biology of the Cell.
I. Molecules & Cells A. Unit One: The Nature of Science a. How is the scientific method used to solve problems? b. What is the importance of controls? c. How does Darwin s theory of evolution illustrate
More informationCurriculum vitae Xigang Liu
Curriculum vitae Xigang Liu 1, EDUCATION: 09/1993-07/1997 B.S. Major: Biology. College of Life Sciences, Hebei Normal University Academic Degree Paper: RAPD analysis of Taigu genic male-sterile wheat and
More informationJMJ14-HA. Col. Col. jmj14-1. jmj14-1 JMJ14ΔFYR-HA. Methylene Blue. Methylene Blue
Fig. S1 JMJ14 JMJ14 JMJ14ΔFYR Methylene Blue Col jmj14-1 JMJ14-HA Methylene Blue Col jmj14-1 JMJ14ΔFYR-HA Fig. S1. The expression level of JMJ14 and truncated JMJ14 with FYR (FYRN + FYRC) domain deletion
More informationPenghui Li, Beibei Chen, Gaoyang Zhang, Longxiang Chen, Qiang Dong, Jiangqi Wen, Kirankumar S. Mysore and Jian Zhao
New Phytologist Supporting Information Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula bhlh transcription factor MtTT8 Penghui Li, Beibei Chen, Gaoyang Zhang, Longxiang
More informationStamford Public Schools Science Department District Midterm Examination REVIEW
Stamford Public Schools Science Department District Midterm Examination REVIEW 2015-2016 Honors Biology Student Name: School/Teacher: Date: SPS Honors Biology Midterm Review, January 2016 Page 1 Dear Biology
More informationSupplemental Data. Hou et al. (2016). Plant Cell /tpc
Supplemental Data. Hou et al. (216). Plant Cell 1.115/tpc.16.295 A Distance to 1 st nt of start codon Distance to 1 st nt of stop codon B Normalized PARE abundance 8 14 nt 17 nt Frame1 Arabidopsis inflorescence
More informationSupplementary Materials for
www.sciencesignaling.org/cgi/content/full/9/452/ra106/dc1 Supplementary Materials for Stem-piped light activates phytochrome B to trigger light responses in Arabidopsis thaliana roots Hyo-Jun Lee, Jun-Ho
More informationPh.D. thesis. Study of proline accumulation and transcriptional regulation of genes involved in this process in Arabidopsis thaliana
Ph.D. thesis Study of proline accumulation and transcriptional regulation of genes involved in this process in Arabidopsis thaliana Written by: Edit Ábrahám Temesváriné Supervisors: Dr. László Szabados
More informationSupplemental Figures. Supplemental Data. Sugliani et al. Plant Cell (2016) /tpc Clades RSH1. Rsh1[HS] RSH2 RSH3. Rsh4[HS] HYD TGS ACT
Supplemental Figures Clades RSH1 TP - HYD TGS ACT Rsh1[HS] RSH2 TP HYD SYN TGS Rsh2[HS] RSH3 TP HYD SYN TGS CRSH TP - SYN EFh Rsh4[HS] Supplemental Figure 1. Arabidopsis RSH domain structure. Schematic
More informationOrganisms: We will need to have some examples in mind for our spherical cows.
Lecture 4: Structure and Composition (Sept. 15) 4.1 Reading Assignment for Lectures 3-4: Phillips, Kondev, Theriot (PKT), Chapter 2 Problem Set 1 (due Sept. 24) now posted on the website. Cellular materials:
More information** * * * Col-0 cau1 CAU1. Actin2 CAS. Actin2. Supplemental Figure 1. CAU1 affects calcium accumulation.
Ca 2+ ug g -1 DW Ca 2+ ug g -1 DW Ca 2+ ug g -1 DW Supplemental Data. Fu et al. Plant Cell. (213). 1.115/tpc.113.113886 A 5 4 3 * Col- cau1 B 4 3 2 Col- cau1 ** * * ** C 2 1 25 2 15 1 5 Shoots Roots *
More informationPlant Molecular and Cellular Biology Lecture 10: Plant Cell Cycle Gary Peter
Plant Molecular and Cellular Biology Lecture 10: Plant Cell Cycle Gary Peter 9/10/2008 1 Learning Objectives Explain similarities and differences between fungal, mammalian and plant cell cycles Explain
More informationFSC-W FSC-H CD4 CD62-L
Supplementary Fig. 1 a SSC-A FSC-A FSC-W FSC-H SSC-W SSC-H CD4 CD62-L b SSC-A FSC-A FSC-W FSC-A FSC-A 7-AAD FSC-A CD4 IL-9 CD4 c SSC-A FSC-A FSC-W FSC-H SSC-W SSC-H 7-AAD KI67 Annexin-V 7-AAD d I L -5
More informationPhotoreceptor Regulation of Constans Protein in Photoperiodic Flowering
Photoreceptor Regulation of Constans Protein in Photoperiodic Flowering by Valverde et. Al Published in Science 2004 Presented by Boyana Grigorova CBMG 688R Feb. 12, 2007 Circadian Rhythms: The Clock Within
More informationUNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE
UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE FACULTY OF HORTICULTURE OANA CIUZAN ROLE OF THE GLYCINE-RICH RNA-BINDING PROTEINS IN PLANT EARLY DEVELOPMENT AND ABIOTIC STRESS RESPONSE ABSTRACT
More informationPlant transformation
Plant transformation Objectives: 1. What is plant transformation? 2. What is Agrobacterium? How and why does it transform plant cells? 3. How is Agrobacterium used as a tool in molecular genetics? References:
More informationCell Death & Trophic Factors II. Steven McLoon Department of Neuroscience University of Minnesota
Cell Death & Trophic Factors II Steven McLoon Department of Neuroscience University of Minnesota 1 Remember? Neurotrophins are cell survival factors that neurons get from their target cells! There is a
More informationLeucine-rich repeat receptor-like kinases (LRR-RLKs), HAESA, ERECTA-family
Leucine-rich repeat receptor-like kinases (LRR-RLKs), HAESA, ERECTA-family GENES & DEVELOPMENT (2000) 14: 108 117 INTRODUCTION Flower Diagram INTRODUCTION Abscission In plant, the process by which a plant
More informationModel plants and their Role in genetic manipulation. Mitesh Shrestha
Model plants and their Role in genetic manipulation Mitesh Shrestha Definition of Model Organism Specific species or organism Extensively studied in research laboratories Advance our understanding of Cellular
More informationSupplemental Figures S1 S5
Beyond reduction of atherosclerosis: PON2 provides apoptosis resistance and stabilizes tumor cells Ines Witte (1), Sebastian Altenhöfer (1), Petra Wilgenbus (1), Julianna Amort (1), Albrecht M. Clement
More informationTNFα 18hr. Control. CHX 18hr. TNFα+ CHX 18hr. TNFα: 18 18hr (KDa) PARP. Cleaved. Cleaved. Cleaved. Caspase3. Pellino3 shrna. Control shrna.
Survival ( %) a. TNFα 18hr b. Control sirna Pellino3 sirna TNFα: 18 18hr c. Control shrna Pellino3 shrna Caspase3 Actin Control d. Control shrna Pellino3 shrna *** 100 80 60 CHX 18hr 40 TNFα+ CHX 18hr
More informationdownstream (0.8 kb) homologous sequences to the genomic locus of DIC. A DIC mutant strain (ro- 6
A B C D ts Figure S1 Generation of DIC- mcherry expressing N.crassa strain. A. N. crassa colony morphology. When a cot1 (top, left panel) strain is grown at permissive temperature (25 C), it exhibits straight
More informationExam 1 ID#: October 4, 2007
Biology 4361 Name: KEY Exam 1 ID#: October 4, 2007 Multiple choice (one point each) (1-25) 1. The process of cells forming tissues and organs is called a. morphogenesis. b. differentiation. c. allometry.
More informationSupplemental Data. Fernández-Calvo et al. Plant Cell. (2011) /tpc
Supplemental Data. Fernández-Calvo et al. Plant Cell. (2011). 10.1105/tpc.110.080788 Supplemental Figure S1. Phylogenetic tree of MYC2-related proteins from Arabidopsis and other plants. Phenogram representation
More informationActions of auxin. Hormones: communicating with chemicals History: Discovery of a growth substance (hormone- auxin)
Hormones: communicating with chemicals History- discovery of plant hormone. Auxin Concepts of hormones Auxin levels are regulated by synthesis/degradation, transport, compartmentation, conjugation. Polar
More informationProcedure to Create NCBI KOGS
Procedure to Create NCBI KOGS full details in: Tatusov et al (2003) BMC Bioinformatics 4:41. 1. Detect and mask typical repetitive domains Reason: masking prevents spurious lumping of non-orthologs based
More information