Alltech Alltima HP Introduction

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1 Alltech Introduction High-Stability, High-Purity, High-Performance, Low-Bleed Columns for Demanding Applications Better Peak Symmetry high-purity silica eliminates peak tailing problems Long Column Life exceptional column stability minimizes downtime and reduces cost Ideal for Microbore or Critical Analysis low to no detectable column bleed Variety of Phases and Formats optimizes retention, resolution, and analysis time Alltima HP combines our best phase chemistries with high-purity silica. The result is one product family with selectivity and performance needed to overcome your most challenging separation needs. Alltech Alltima HP Phase Specifications Pore Size Surface Area Carbon Load Endcapped? Phase Description Base Particle Particle Size Phase USP C8 For classic reversed-phase applications Silica Spherical, μm 90Å 00 m /g % Monomeric Yes L C8 EPS Reversed-phase C8 with extended polar selectivity Silica Spherical, μm 90Å 00 m /g % Monomeric Yes L C8 HiLoad Higher carbon load for stronger retention Silica Spherical, μm 00Å 0 m /g % Monomeric Yes L C8 AQ 00% water wettable Silica Spherical, μm 00Å 0 m /g 0% Monomeric Yes L C8 Amide Low bleed polar-embedded phase compatible Silica Spherical, μm 90Å 00 m /g % Monomeric Yes L with microbore C8 For reversed-phase applications where C8 is Silica Spherical, μm 90Å 00 m /g 8% Monomeric Yes L7 too retentive Cyano Stable, long-life cyano phase Silica Spherical, μm 90Å 00 m /g % Monomeric Yes L0 Silica For general purpose normal-phase applications Silica Spherical, μm 00Å 0 m /g No L HILIC Hydrophillic Interaction Chromatography for highly polar analytes Silica Spherical.,, μm 0Å 0 m /g L Basic Compounds at Neutral ph 0 7 Min. The Alltima HP phases demonstrate excellent peak symmetry of highly basic analytes at ph 7.0. Peak USP Tailing*. Diphenhydramine.9. Nortriptyline.. Amitriptyline.7 *Tailing Factor was determined in accordance with USP method. Column: Alltima HP C8, μm,. x 0mm Mobile Phase: mm Potassium Phosphate, ph 7.0:Methanol (:8) Flow Rate:.0mL/min Detector: UV at nm Basic Compound Stability at ph and ph0 Final Acid Final Base Retention Times. Uracil Phenol N,N-Diethyl-m-Toluamide Toluene Column: Alltima HP C8, μm,. x 0mm Mobile Phase: Acetonitrile:Water (8:) Flow Rate:.0mL/min Detector: UV at nm Final Acid: washed with,000 column volumes of Acetonitrile:Dilute Sulfuric Acid, ph.0 (0:0) at 0 C. Final Base: washed with,000 column volumes of Acetonitrile:Ammonium Hydroxide, ph 0 (0:0) at 0 C. Initial 0 Min. The Alltima HP phases demonstrate exceptional stability from ph.0 to

2 Alltech Introduction The Alltima HP Family Demonstrates Low Bleed Alltima HP columns are among the lowest-bleed columns available, making them ideal for microbore applications. Our C8 Amide polar-embedded phase has significantly lower column bleed than competitive amide columns. Relative Abundance Level (X0 ) 0 8 Column Bleed by ESI-MS Alltima HP vs. Competitive Columns Alltech Competitors No Column Silica C8 C8 HiLoad C8 MS C8 MS C8 Amide C8 Amide C8 Amide Method Development Kits best value Three Different Kits with Different Selectivities Whether you are developing a new method, or just not satisfied with an existing separation, the Alltima HP family can help optimize your separation. Your best value for method development purchase a kit and receive three columns for the price of two Alltima HP Method Development Kits Description Includes: i.d. x Length Part No. Kit C8 Mix, μm Expedite MS Kit C8, C8 HiLoad, C8 Amide (877, 879, 8778). x 0mm 878 Analytical Kit C8, C8 HiLoad, C8 Amide (878, 878, 877). x 0mm 8780 Rocket Kit C8, C8 HiLoad, C8 Amide (877, 8790, 877) 7 x mm 878 Kit Alternate Mix, μm Expedite MS Kit C8 HiLoad, C8, CN (879, 877, 877). x 0mm 878 Analytical Kit C8 HiLoad, C8, CN (878, 8770, 8770). x 0mm 8780 Rocket Kit C8 HiLoad, C8, CN (8790, 877, 877) 7 x mm 878 Kit Polar Mix, μm Expedite MS Kit C8 EPS, C8 Amide, CN (8770, 8778, 877). x 0mm 8787 Analytical Kit C8 EPS, C8 Amide, CN (8770, 877, 8778). x 0mm Rocket Kit C8 EPS, C8 Amide, CN (87708, 877, 877) 7 x mm 8787 tech tip Want high throughput on a conventional (non-ultra high-pressure) system? Choose the Rocket column format no modifications necessary to your existing HPLC system. Choose Expedite columns for low volume and microbore systems. See page for more explanation of the Rocket and Expedite formats. 9

3 0 Alltech Reversed-Phase Columns Selectivity Options in One Product Family C8: Classic reversed-phase retention and selectivity C8 AQ: 00% water wettable and high carbon loading for greater mobile phase range EPS C8: Unique selectivity that succeeds when traditional reversed-phase columns fail Greater retention and enhanced peak symmetry for polar compounds C8 HiLoad: Superior retention and loadability for resolution of complex samples C8 Amide: The first polar-embedded packing ideal for microbore Excellent peak shape without phase bleed Ideal for basic compounds in neutral to alkaline ph C8: Less hydrophobic retention than C8 Alltima HP Selectivity Alltima HP Packing Acid, Base, and Neutral Test Mix 0 8 Min. Column:. x 0mm Mobile Phase: 0mM KHPO, ph.0:chcn (0:0) Column Temp: Ambient ( C) Flow Rate:.0mL/min Injection Vol.: μl Detector: UV at nm Uracil. Pyridine. Phenol. N,N-diethyl-m-toluamide. -Butyl Benzoic Acid. Toluene C8 EPS C8 C8 HiLoad Alltima HP HPLC Columns C8, μm* Capillary 0.0 X 0mm Capillary 0.0 X00mm Capillary 0.0 X0mm Capillary 0.00 X 0mm Capillary 0.00 X00mm 7 Capillary 0.00 X0mm 8 Expedite MS. x 0mm 877 Expedite MS. x 0mm 877 Microbore. x 0mm 870 Microbore. x 00mm 879 Microbore. x 0mm 8770 Solvent-Reducer.0 x 0mm 870 Expedite MS. x 0mm 877 Expedite MS. x 0mm 877 Analytical. x 0mm 878 Analytical. x 00mm 877 Analytical. x 0mm 878 Rocket 7 x mm 877 Rocket 7 x mm 877 C8, μm Microbore. x 0mm 878 Microbore. x 0mm 878 Solvent-Reducer.0 x 0mm 870 Solvent-Reducer.0 x 0mm 870 Analytical. x 0mm 8779 Analytical. x 0mm 8780 C8 AQ, μm* Capillary 0.0 x 0mm 0 Capillary 0.0 x 00mm Capillary 0.0 x 0mm Capillary 0.00 x 0mm Capillary 0.00 x 00mm Capillary 0.00 x 0mm Expedite MS. x 0mm 878 Expedite MS. x 0mm 878 Microbore. x 00mm Microbore. x 0mm 8780 Expedite MS. x 0mm 878 Expedite MS. x 0mm 878 Analytical. x 0mm 878 Analytical. x 00mm Analytical. x 0mm Rocket 7 x mm 878 Rocket 7 x mm 878 C8 AQ, μm Microbore. x 0mm 878 Microbore. x 0mm 878 Analytical. x 0mm 8789 Analytical. x 0mm 8780 C8 EPS, μm Expedite MS. x 0mm Expedite MS. x 0mm 8770 Microbore. x 0mm 8708 Microbore. x 00mm 8770 Microbore. x 0mm 8770 Solvent-Reducer.0 x 0mm 870 Expedite MS. x 0mm 877 Expedite MS. x 0mm 877 Analytical. x 0mm 878 Analytical. x 00mm 8770 Analytical. x 0mm 8770 Rocket 7 x mm Rocket 7 x mm C8 EPS, μm Microbore. x 0mm 8777 Microbore. x 0mm 8778 Solvent-Reducer.0 x 0mm 870 Solvent-Reducer.0 x 0mm 870 Analytical. x 0mm 877 Analytical. x 0mm 877 *Other particle sizes and dimensions are available.

4 Alltech Reversed-Phase Columns Alltima HP HPLC Columns (continued) C8 Hi-Load, Capillary 0.0 x 0mm 90 μm* Capillary 0.00 x 0mm 9 Expedite MS. x 0mm 879 Expedite MS. x 0mm 879 Microbore. x 0mm 870 Microbore. x 00mm 8787 Microbore. x 0mm 8788 Solvent-Reducer.0 x 0mm 870 Expedite MS. x 0mm 879 Expedite MS. x 0mm 879 Analytical. x 0mm 8787 Analytical. x 00mm 878 Analytical. x 0mm 878 Rocket 7 x mm 8789 Rocket 7 x mm 8790 C8 Hi-Load, Microbore. x 0mm 8799 μm Microbore. x 0mm Solvent-Reducer.0 x 0mm 87 Solvent-Reducer.0 x 0mm 87 Analytical. x 0mm 8797 Analytical. x 0mm 8798 C8 Amide, Capillary 0.0 x 0mm μm* Capillary 0.00 x 0mm 8 Expedite MS. x 0mm 8777 Expedite MS. x 0mm 8778 Microbore. x 0mm 870 Microbore. x 00mm 877 Microbore. x 0mm 877 Solvent-Reducer.0 x 0mm 8707 Expedite MS. x 0mm 8779 Expedite MS. x 0mm 8770 Analytical. x 0mm 8789 Analytical. x 00mm 877 Analytical. x 0mm 877 Rocket 7 x mm 877 Rocket 7 x mm 877 C8 Amide, Microbore. x 0mm 877 μm Microbore. x 0mm 877 Solvent-Reducer.0 x 0mm 8708 Solvent-Reducer.0 x 0mm 8709 Analytical. x 0mm 877 Analytical. x 0mm 877 C8, μm* Capillary 0.0 x 0mm 79 Capillary 0.0 x 00mm 80 Capillary 0.0 x 0mm 8 Capillary 0.00 x 0mm 8 Capillary 0.00 x 00mm 8 Capillary 0.00 x 0mm 8 Expedite MS. x 0mm 877 Expedite MS. x 0mm 877 Microbore. x 0mm 87 Microbore. x 00mm 877 Microbore. x 0mm 877 Solvent-Reducer.0 x 0mm 87 Expedite MS. x 0mm 8777 Expedite MS. x 0mm 8778 Analytical. x 0mm 8780 Analytical. x 00mm 8779 Analytical. x 0mm 8770 Rocket 7 x mm 877 Rocket 7 x mm 877 C8, μm Microbore. x 0mm 877 Microbore. x 0mm 877 Solvent-Reducer.0 x 0mm 87 Solvent-Reducer.0 x 0mm 87 Analytical. x 0mm 877 Analytical. x 0mm 877 *Other particle sizes and dimensions are also available. Alltima HP-Guard Cartridges Packing i.d. x Length Qty. Part No. C8 Capillary Guard, μm** 0.0 x 0mm ea x 0mm ea 79 C8 All-Guard, μm*. x 7.mm x 7.mm 87. x 7.mm 878 C8 AQ Capillary Guard, μm** 0.0 x 0mm ea x 0mm ea 9 C8 AQ All-Guard, μm*. x 7.mm 878. x 7.mm 878 C8 EPS All-Guard, μm*. x 7.mm x 7.mm 87. x 7.mm 8770 C8 Hi-Load Capillary 0.0 x 0mm ea 88 Guard, μm** 0.00 x 0mm ea 89 C8 Hi-Load All-Guard, μm*. x 7.mm x 7.mm 87. x 7.mm 8770 C8 Amide Capillary 0.0 x 0mm ea 98 Guard, μm** 0.00 x 0mm ea 99 C8 Amide All-Guard, μm*. x 7.mm x 7.mm 87. x 7.mm 8778 C8 Capillary Guard, μm** 0.0 x 0mm ea x 0mm ea 8 C8 All-Guard, μm*. x 7.mm x 7.mm 877. x 7.mm 877 All-Guard Cartridge Holder ea 800 (Includes Direct-Connect Column Coupler) Capillary Guard Cartridge Holder Guard Holder for 0.00mm and 0.0mm Guards ea GR-70E Guard Holder for 0.00mm and 0.00mm Guards ea GR-70A *All-Guard holder required. **Other particle sizes and dimensions are available. tech tip Which guard format should I choose? Ideally, the guard should have the same or slightly smaller i.d. as the HPLC column. Efficiency will suffer, if the guard i.d. is larger than that of the column. technical assistance Contact Tech Support: Phone: (North America) Online:

5 Alltech HILIC Columns Hydrophilic Interaction (HILIC) Columns for Highly Polar Basic Analytes Superior retention of highly polar compounds Increased sensitivity and lower detection limits with microbore Available in.μm particle size Hydrophilic Interaction Chromatography is ideal for separating and retaining very polar compounds that may not retain on traditional reversed-phase packings. Unlike reversed-phase columns, HILIC columns retain highly polar compounds with only small amounts of water in the mobile phase. These more volatile mobile phases increase sensitivity with microbore applications. Basic Drugs Reverse-Phase C8 Columns Min. Column: Mobile Phase: Flow Rate: Detector: Mixed Analyte HILIC. Toluene. Hydantoin. Uracil. Caffeine. Allantoin Alltima HP HILIC, μm,. x 0mm (Part No. 8) Acetonitrile:0.0M Ammonium Formate, ph.0 (9:).0mL/min UV at 0nm 0 Alternate selectivity compared to reversed-phase columns allows more separation possibilities. HILIC Columns Min.. Albuterol. Bamethan Column: Prevail C8 Column, μm,. x 0mm (Part No. 9908) Alltima HP HILIC Column, μm,. x 0mm, (Part No. 8) Mobile Phase: 0.M Ammonium Formate, ph.0:chcn (88:) Acetonitrile:0.M Ammonium Formate, ph.0 (8:) Flow Rate:.0mL/min Detector: UV at 0nm Use Alltima HP HILIC's alternate selectivity for greater separation possibilities. tech tip Want high throughput on a conventional (non-ultra high-pressure) system? Choose the Rocket column format no modifications necessary to your existing HPLC system. Choose Expedite columns for low volume and microbore systems. See page for more explanation of the Rocket and Expedite formats. Rocket Columns 90 Expedite Columns Alltima HP HILIC HPLC Columns HILIC,.μm Expedite MS. x 0mm 87 Expedite MS. x 0mm 87 Rocket 7 x mm 87 Rocket 7 x mm 88 HILIC, μm* Capillary 0.0 x 0mm Capillary 0.0 x 00mm Capillary 0.0 x 0mm 7 Capillary 0.00 x 0mm 8 Capillary 0.00 x 00mm 9 Capillary 0.00 x 0mm 0 Expedite MS. x 0mm 87 Expedite MS. x 0mm 87 Microbore. x 0mm 8 Microbore. x 0mm 8 Expedite MS. x 0mm 87 Expedite MS. x 0mm 87 Analytical. x 0mm 80 Analytical. x 0mm 8 Rocket 7 x mm 89 Rocket 7 x mm 870 HILIC, μm Microbore. x 0mm 8 Analytical. x 0mm 8 Analytical. x 0mm 8 *Other particle sizes and dimensions are available. Alltima HP HILIC Guard Cartridges Packing i.d. x Length Qty. Part No. HILIC Capillary Guard, 0.0 x 0mm ea 8 μm** 0.00 x 0mm ea 9 HILIC All-Guard, μm*. x 7.mm 879. x 7.mm 880 All-Guard Cartridge Holder ea 800 (Includes Direct-Connect Column Coupler) Capillary Guard Cartridge Holder Guard Holder for 0.00mm and 0.0mm Guards ea GR-70E Guard Holder for 0.00mm and 0.00mm Guards ea GR-70A *All-Guard holder required. **Other particle sizes and dimensions are available.

6 Alltech Normal-Phase Columns Cyano: Superior stability More reproducible separations than silica for normal-phase applications Ideal for basic drug analysis Silica: Normal phase % Changes in Retention QC D Test: Alltima HP Cyano Much More Stable than Other Cyanos ph.0 ph 7. CN Brand A CN Brand B CN Brand C CN Brand D CN Brand E CN Brand F CN 70:0 HO/CHCN,.0mL/min, UV at nm, μl DMix, Column. x 0mm, C ph.0 Stability: Flushed with HSO ph.0:acetonitrile 0:0 at.0ml/min for days ph 7. Stability: Flushed with 0mM KHPO ph 7.:Acetonitrile 0:0 at.0ml/min for days Alltima HP HPLC Columns Cyano, μm Expedite MS. x 0mm 877 Expedite MS. x 0mm 877 Microbore. x 0mm 87 Microbore. x 00mm 8779 Microbore. x 0mm 8770 Solvent-Reducer.0 x 0mm 87 Expedite MS. x 0mm 877 Expedite MS. x 0mm 877 Analytical. x 0mm 878 Analytical. x 00mm 8777 Analytical. x 0mm 8778 Rocket 7 x mm 877 Rocket 7 x mm 877 Cyano, μm Microbore. x 0mm 8778 Microbore. x 0mm 8778 Solvent-Reducer.0 x 0mm 877 Solvent-Reducer.0 x 0mm 878 Analytical. x 0mm 8779 Analytical. x 0mm Silica, μm Expedite MS. x 0mm 8779 Expedite MS. x 0mm 8779 Microbore. x 0mm 87 Microbore. x 00mm Microbore. x 0mm Solvent-Reducer.0 x 0mm 879 Expedite MS. x 0mm 8779 Expedite MS. x 0mm 8779 Analytical. x 0mm 878 Analytical. x 00mm 8778 Analytical. x 0mm 8778 Rocket 7 x mm Rocket 7 x mm Deoxycorticosterone Acetate. Hydrocortisone Acetate. Cortisone Acetate. Cortisone Corticosteroids Min. Column: Alltima HP CN, μm,. x 0mm (Part No. 8779) Mobile Phase: Hexane:Isopropanol (9.7:.) Flow Rate:.0mL/min Column Temp: C Detector: UV at nm. Prednisone. Hydrocortisone 7. Prednisolone 7 09 Alltima HP HPLC Columns (continued) Silica, μm Microbore. x 0mm Microbore. x 0mm 8780 Solvent-Reducer.0 x 0mm 870 Solvent-Reducer.0 x 0mm 87 Analytical. x 0mm Analytical. x 0mm Alltima HP All-Guard Cartridges* Packing i.d. x Length Qty. Part No. Cyano, μm. x 7.mm x 7.mm 87. x 7.mm 8778 Silica, μm. x 7.mm x 7.mm 878. x 7.mm 8780 All-Guard Cartridge Holder ea 800 (Includes Direct-Connect Column Coupler) *All-Guard holder required. Other particle sizes available. more applications To view our complete searchable chromatogram database visit

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