An HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint

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1 An HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint An important new column in the Inertsil series Bonded-Phase Structure Perfect balance of retention for both polar and nonpolar compounds. / of Inertsil ODS- retention for Hydrophobic compounds. Special new Sprint HG column hardware improves peak shape and efficiency.x0mm Sprint HG column is ideal for High-Throughput LC/MS Separations. Superior Inertness. Compatible with 00% aqueous eluents. -(CH ) CH Base Silica Physical Properties and Chemical Modification Particle Size: um Particle Shape: Spherical Specific Surface Area: 0m/g Pore Size: 00A Pore Volume:.0mL/g Purity: % Bonded Phase: Octadecyl groups Endcapped: Yes Carbon Load: 8.% Recommended ph Range: to. USP: L Guaranteed Theoretical Plate Numbers for 0 and 0mmm Column Length: 00,000/m QC Tests Employed: SEM, M, Ph, C, Schro, T, Py, Am, Ac, Cu, D, CP Perfectly Balanced Retention Our newly-developed chemical bonding technology allows Inertsil ODS Sprint to retain hydrophilic compounds (see peaks to, below) without excessive retention of non-polar compounds (see peaks to ). Hydrophobic compounds can be eluted quickly 6 um.6x0mm 6 System: GL00 HPLC system Eluent CH OH / H O = 80 / 0 Flow rate: Col.Temp.: Detection: ) Uracil ) Caffein ) Phenol ) n-butylbenzene ) o-terphenyl 6) n-amylbenzene ) Triphenylene.0 ml/min 0C nm Inertsil ODS- um.6x0mm

2 Sprint HG High Performance Column Hardware To help maximize the performance of Inertsil Sprint, GL Sciences developed a completely new column hardware design, called Sprint HG hardware. This hardware provides far less column dead-volume compared to other designs, resulting in higher theoretical number of plates and better peak shape. Sprint HG column hardware guarantees at least 00,000 plates/m for 0 and 0mm column lengths. The.X0mm Sprint HG column combines sharp peaks with fast elution time and is ideal for High-Throughput LC/MS Separations. New HG type hardware The current hardware EX type 0mm length column can be packed too!!! A) B) A) um.6x0mm B) Inertsil ODS- um.6x0mm Theoretical Plates Number of Peak No. =,9 Sharper Peaks!!! Theoretical Plates Number of Peak No. =,0 System: GL00 HPLC system Eluent: A) CH CN B)0.%H PO A:B=9: Flow rate:.0 ml/min Col. Temp.: 0 Detection: nm Injection Vol.: ml. Sudan (0.ug/mL). Sudan (0.ug/mL). Sudan (0.ug/mL). Sudan (0.ug/mL) Superior Inertness Inertsil Sprint is created by using a completely new bonding technique invented by GL Sciences. After bonding octadecyl silane (ODS) to highly purified base-silica gel, intensive end-capping is performed to eliminate residual silanols, creating an exceptionally inert column for reverse phase chromatography. Inertsil ODS Sprint phase shows excellent peak shapes for a wide range of polar analytes, including both basic compounds and acidic compounds, as shown in the chromatograms below. See the difference for yourself!!!

3 Pyridine Test for Base Deactivation Column Length:.6X0mm um Eluent CHOH / HO = 0 / 0 Flow Rate:.0 ml/min Col.Temp.: 0C Detector:: UVnm Sample Volume: ul Samples: ) Pyridine 0.09 mg/ml ) Phenol 0. mg/ml Symmetry C SunFire C XTerra MS C Atlantis dc Luna ODS() Gemini C Synergi MAX-RP Zorbax Eclipse XDB-C8

4 Aminopyridine Test for Base Deactivation Column Length:.6X0mm um Eluent CHOH / 0mM Phosphate Buffer (ph.6) 0:90 Flow Rate:.0 ml/min Col.Temp.: 0C Detector:: UVnm Sample Volume: ul Samples: ) -Aminopyridine 0.mg/mL ) -Aminopyridine.0mg/mL ) -Aminopyridine.0mg/mL Symmetry C8 0 0 SunFire C XTerra MS C Peak & are Compared to Peak, Peak & are absorbed. 0 0 Atlantis dc8 0 0 Luna ODS() 0 0 Gemini C Synergi MAX-RP 0 0 Zorbax Eclipse XDB-C8

5 Carboxylic Acid Test for Silanol Activity Column Length:.6X0mm um Mobile Phase: 0.% HPO(v/v) Flow Rate:.0 ml/min Col.Temp.: 0C Detector: UV0nm Sample Volume: ul Samples: ) Formic Acid 0. %(v/v) ) Acetic Acid 0. %(v/v) Symmetry C SunFire C XTerra MS C Formic acid is Atlantis dc8 Luna ODS() Gemini C Formic acid is Synergi MAX-RP Zorbax Eclipse XDB-C8

6 Test for Silica Purity Column Length:.6X0mm um Eluent CHOH / 0mM HPO (0/90, w:w) Flow Rate:.0 ml/min Col.Temp.: 0C Detector:: UVnm Sample Volume:.uL Samples: ) 0.0 mg/ml ) Caffeine 0. mg/ml Symmetry C8 0 0 Atlantis dc Luna ODS() SunFire C XTerra MS C8 0 0 Gemini C8 0 0 Synergi MAX-RP 0 0 Zorbax Eclipse XDB-C8

7 Compatible with 00% aqueous eluents Inertsil ODS Sprint is designed to have enhanced retention of polar compounds without an embedded polar group in the bonded phase ligand. To retain water-soluble organic compounds, the use of mobile phases that contain little or no organic modifier are sometimes needed. Under these highly aqueous conditions, conventional C8 columns often show sudden loss of retention, known as phase collapse. This phenomenon is caused by dewetting of the bonded phase inside the pores of the silica. The following data shows the results of a stop-flow test, which demonstrates Inertsil ODS Sprint s resistance to dewetting and phase collapse. Column:.6X0MM um Eluent: Water 00% Col.Temp.: 0C Flow rate:.0ml/min Detection: UVnm st analysis rd analysis ) Cytosine ) Uracil ) guanine ) thymine ) adenine Testing Procedure ) The st analysis was conducted after introducing the eluent for 0 minutes. ) Stop flow for minutes. ) The nd analysis was conducted after introducing the eluent for 0 minutes. ) Stop flow for minutes. ) The rd analysis was conducted after introducing the eluent for 0 minutes. st analysis,, rd analysis st analysis rd analysis,, st analysis rd analysis, Commercially Available ODS Column A Commercially Available ODS Column B Commercially Available ODS Embedded Column C Applications Analysis of Cold Medicine Analytical Condition System: GL-00 HPLC System Column: um 0X.6 mm I.D. Mobile Phase: A) Acetonitrile B) 0.% Phosphoric acid (A/B) = (0/90)-min-(0/90)-0min- (00/0) Flow Rate:.0 ml/min Column Temp: 0C Detector: UV at 0nm Sample Volume: 0uL (each 0mg/L) ) Potassium guaiacolsulfonate ) Acetaminophen ) Caffeine ) Ethenzamide ) Allyl isopropyl acetyl urea 6) Isopropylantipyrine ) Ibuprofen 6 Analysis of Food Colorants Analytical Condition System: GL-00 HPLC System Column: um 0X.6 mm I.D. Mobile Phase: A) Acetonitrile B) 0mM Phosphate buffer (ph6.9) (A/B) = (0/90)-0min-(/6) Flow Rate:.0 ml/min Column Temp.: 0C Detector: UV at 0nm Sample Volume: 0uL ) Acid Yellow No..6mg/L ) Acid Red No..8mg/L ) Acid Blue No..6mg/L ) Acid Red No.0.8mg/L ) Acid Yellow No..mg/L 6) Uranine.8mg/L ) Acid Red No.0.mg/L 8) Ponceau R.6mg/L 9) Ponceau SX.mg/L 0) Orange.mg/L ) Acid Green No..0mg/L ) Acid Blue No..0mg/L ) Acid Red No..mg/L ) Azul Blue VX.0mg/L ) Orange.6mg/L 6) Acid Red No.06.0mg/L Analysis of Catechins Analytical Condition System: GL-00 HPLC System Column: um 0X.6 mm I.D. Mobile Phase: A) Methanol B) 0mM Sodium dihydrogen phosphate (A/B) = (0/90)-0min-(0/0) Flow Rate:.0 ml/min Column Temp.: 0C Detector: UV at 80nm Sample Volume: 0uL (each mg/l) ) Gallocatechin (GC) )Epigallocatechin (EGC) ) Catechin (C) ) Epigallocatechin gallate (EGCg) ) Epicatechin (EC) 6) Gallocatechin gallate (GCg) ) Epicatechin gallate (ECg) 8) Catechin gallate (Cg) Analysis of Antibacterial Agents by LC/MS/MS Analytical Condition System: Symbiosis System Column: m 0x. mm I.D. Mobile Phase: A) Methanol B) 0.mM Ammonium acetate (A/B) = (0/0)-min-(90/0) Flow Rate: 0. ml/min Column Temp.: 0C Detector: API000 LC/MS/MS System ESI, Positive Sample Volume: 0uL (each 0ug/L) ) -NBA-AHD )-NBA-AOZ ) -NBA-SEM ) -NBA-AMOZ

8 Ordering Information ID(mm)..6 Length(mm) Cat.No. Cat.No Price * HG type hardware are used for all the sizes above. * End-fitting is Waters /6 type. * For other column sizes, please give us an enquiry. (Upon request) Cartridge Guard Column E Ordering Information Analytical Column I.D.mm Size Cartridge Guard Column E Guard Column I.D.mm Size Guard Column Length mm Size Cartridge Guard Column E (EA. / Set) Cat.No. Price Cat.No. Price. mm.0 mm 0 mm mm.0 mm 0 mm * When ordering, please specify the packing material and the particle size. * Only Waters /6 end-fitting type is compatible for our Cartridge Guard Column E. * Conventional Guard Columns and GL-Cart Guard Columns are also available. For more information, please feel free to contact us. * EX type hardware are used for all the Cartridge Guard Column E columns. The specification and the column type are subject to change without notice due to continual improvements. All brand names and product names are trademarks or registered trademarks of GL Sciences Inc. TM or are not described in this brochure. Cartridge Guard Column E Holder Set ( Cartridges & Holder) To find your local distributor, please visit to our website Simply select your country from the list and your local distributor Information will be displayed. ISO00: Save Energy and Resources!! Reduce Amount of Eluent with Smaller ID Inertsil Columns!!