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1 mau ph - 6 8
2 TAKE A LOOK INSIDE TWIN Technology TWIN (Two-In-One ) Technology is what gives Gei its superior performance edge. During the final stage of silica manufacturing, a unique silica-organic layer is grafted to create a completely new composite particle. Since the internal base silica is unaltered by this manufacturing process, the particle retains the mechanical strength and rigidity of silica, providing excellent efficiency, while the silica-organic shell protects the particle from chemical attack. It s About Options: Extended ph Stability (-) Efficiency of Silica Mechanical Strength of Silica % Aqueous Stable Material Characteristics: Packing Material Particle Shape/Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load End Capping Gei C8 Spherical,5, 75 % TMS Gei C6-Phenyl Spherical,5 75 % TMS FORM FOLLOWS FUNCTION Format Options Gei media is available in a variety of formats to match your chromatographic needs, whether it s high-sensitivity narrow bore columns, superior efficiency analytical columns, or preparative scale Axia packed technology ph-lc Extended ph - Stability for Alternate Selectivity ph.5. % TFA in Water ph. mm Ammonium Formate ph 7. mm Phosphate Buffer APP ID 557 APP ID 558, APP ID 559 Poor Resolution for Basic Compounds,,5, Phenomenex Web:
3 REPRODUCIBLE PHASES From Batch-to-Batch Gei C8 Even under low buffer conditions and at high ph, Gei provides the stability and reproducibility needed for developing and transferring critical methods. With each Gei phase and particle size, multiple batches are available for validating methods. In addition, Gei is available and supported in 7 countries. Gei C6-Phenyl Gei C6-Phenyl incorporates the same tightly controlled silica manufacturing procedures as our Gei C8 phase to deliver reproducible performance you can depend on. Column: Gei 5 µm C8 Dimension: 5 x.6 mm Part No.: F-5-E Mobile Phase: mm Ammonium Bicarbonate, ph.5/acetonitrile (5:5) Flow Rate:. ml/ Detection: nm Sample:. Pindolol. Metoprolol. Propanolol Column: Gei 5 µm C6-Phenyl Dimension: 5 x.6 mm Part No.: F--E Mobile Phase: mm Phosphate buffer, ph.5/methanol (97:) Flow Rate:. ml/ Detection: nm Sample:. Tartaric acid. Malic acid. Lactic acid. Acetic acid 5. Citric acid 6. Propionic acid Batch # Batch # Batch # Batch # Batch # Batch # times increase in stability* Column: Gei 5 µm C8 Dimension: 5 x.6 mm Part No.: F-5-E Mobile Phase: Acetonitrile/Buffers at various ph s (see chromatograms), (5:5) Flow Rate:. ml/ Temperature: C Detection: 5 nm Sample:. Chlorpropamide 5.). Butylparaben. Lidocaine 7.9). Triprolidine 6.5) 5. Dextromethorphan 9.) *Compared to a traditional column with a ph - range. 56 ph. mm Ammonium Bicarbonate Buffer APP ID 56 Poor Resolution for Basic Compounds ph. mm Triethylae Buffer APP ID 56 Did you know that by modifying the mobile phase ph, alternative selectivity can be achieved with ionizable compounds? Acidic compounds will typically have an increase in retention at a ph below their pk a value, while basic compounds will typically have a increase in retention when the ph is above their pk a value. Shown above is an example of this behavior and how, by modifying the ph, one can modify selectivity. Contact any Phenomenex office for additional support on ph Method Development!
4 ph SELECTIVITY Because the majority of pharmaceutically active compounds are ionizable (the uncharged species exhibit longer retention on reversed phase media), modifying the mobile phase ph is a powerful tool for manipulating retention and selectivity. Operating at these conditions: For Acidic Compounds: For Basic Compounds: Operate ph units below pk a Operate ph units above pk a Improve Peak Shape & Increase Retention Cold Products Gei C8 Mobile Phase: mm Potassium Phosphate, ph.5/acetonitrile (75:5) mau 6 mau 6 Dimension: 5 x.6 mm ph.5 Part No: F-5-E ph 7. ph. ph.5. Triprolidine Mobile Phase: mm Ammonium Bicarbonate, ph./acetonitrile (6:) APP ID APP ID 5 Flow Rate:.5 ml/ Detection: nm Temperature: 5 C Sample:. Maleic acid.8, 6.7). Chlorphenirae., 9.) 6.5). Diphenhydrae 9.) Tricyclic Antidepressants Gei C6-Phenyl Mobile Phase: mm Phosphate Buffer, ph 7./Acetonitrile/Methanol (:5:5) mau mau 6 8 Dimension: 5 x.6 mm Part No: F--E Flow Rate:.5 ml/ Detection: 5 nm Temperature: 5 C Sample:. Imiprae 9.5). Nortriptyline 9.7). Amitriptyline 9.). Clomiprae 9.5) Mobile Phase: mm Ammonium Bicarbonate, ph.5/acetonitrile/methanol (:5:5) APP ID APP ID 5885 AROMATIC SELECTIVITY Phenyl phases offer another group of selectivity options. π-π interactions between aromatic groups can influence retention order of aromatic compounds. Varying the organic mobile phase can modulate such interactions: Acetonitrile Reduces Aromatic Interactions Methanol Increases Aromatic Interactions Depending on your separation needs, the use of either acetonitrile or methanol with Gei C6-Phenyl provides additional options to improve your separation. % Acetonitrile 5 % Acetonitrile 6 8, 55 % Methanol APP ID 5896 APP ID 5895 Flavonoids Column: Gei 5 µm C6-Phenyl Å Dimension: 5 x.6 mm Part No.: F--E Mobile Phase:. % Formic Acid in Water/Organic as noted Flow Rate:. ml/ Detection: 5 nm Sample:. Quercetin. Kaempferol. Isorhamnetin % Methanol APP ID 59 APP ID 59 Food Additives Column: Gei 5 µm C6-Phenyl Å Dimension: 5 x.6 mm Part No.: F--E Mobile Phase: mm Potassium Phosphate, ph.5/ Organic as noted Flow Rate:. ml/ Detection: nm Sample:. Saccharin. p-hydroxybenzoic acid. Sorbic acid. Dehydroacetic acid 5. Methylparaben Improved Separation! 5 Different Selectivity! Phenomenex Web:
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