Ion Exchange Columns ---- Inertsil AX 078 Inertsil CX 080. HPLC Column Catalog

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1 Ion Exchange ---- Inertsil AX 078 Inertsil CX 080 HPLC Column Catalog 077

2 Inertsil AX Physical Properties Silica : Series High Purity Silica Gel : μm Surface Area : 0 m /g Pore Size : 00 Å (0 nm) Pore Volume :.0 ml/g Bonded Phase : Diethylaminopropyl Groups End-capping : one Carbon Loading : 7 % AEC : 0. meq/g USP Code : - ph Range : ~ 7. Inertsil AX has diethylamino groups bonded to silica gel by an alkyl chain. The diethylamino groups offer anionic functions required for anion exchange chromatography. It is mainly used for analyses of acidic compounds. Conventional ion-exchange columns used to show inconsistent results from lot to lot. However, Inertsil AX is manufactured under strict quality control in order to offer excellent lot-to-lot reproducibility. The retentivity of Inertsil AX is infl uenced by the concentration of buffer. The retention time can be adjusted by the concentration of buffer (Refer to Fig. ). Figure : Comparison of lot-to-lot reproducibility with other brands Lot. A Lot. A Column Size : μm, 0.6 mm I.D. Eluent : 60 mm KHP (ph.0, HP) Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV nm Injection Vol. Sample : μl :. Cytidine -monophosphate (CMP). Uridine -monophosphate (UMP) H Lot. B Lot. B H Lot. C Lot. C H P H P 0 Inertsil AX other brand s ion-exchange column H H Cytidine -monophosphate (CMP) H H Uridine -monophosphate (UMP) Figure : Effect of buffer concentration in eluent Eluent : 0 mm KHP (ph.0, HP) Eluent : 0 mm KHP (ph.0, HP) Column Flow Rate Col. Temp. Detection Injection Vol. Sample : Inertsil AX ( μm, 0.6 mm I.D.) :.0 ml/min : 0 C : UV nm : 0 μl :. Cytidine -monophosphate (CMP). Adenine -monophosphate (AMP). Uridine -monophosphate (UMP). Guanosine -monophosphate (GMP). Xanthosine -monophosphate (XMP) Eluent : 0 mm KHP (ph.0, HP) HPLC Column Catalog

3 Inertsil AX Analytical : μm Length \ I.D Length \ I.D * End-fi ttings are /6 Waters-compatible. * For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm μm , , , , , Holder for Cartridge Guard Column E * End-fi ttings are /6 Waters-compatible. * For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC ormal Phase SEC Ion Exchange Application Specifi c Guard Preparative Capillary Applications Cat. o. Index HPLC Column Catalog 079

4 Inertsil CX Physical Properties Silica : Series High Purity Silica Gel : μm Surface Area : 0 m /g Pore Size : 00 Å (0 nm) Pore Volume :.0 ml/g Bonded Phase : Benzensulfonyl Groups End-capping : one Carbon Loading : % CEC : 0. meq/g USP Code : L9 ph Range : ~ 7. Inertsil CX has benzenesulfonyl groups bonded to silica gel by an alkyl chain. The sulfony groups at the end of the structure offer cationic functions required for the cation exchange chromatography. It is mainly used for analyses of basic compounds. Inertsil CX is manufactured under strict quality control in order to offer excellent lot-to-lot reproducibility as the same as Inertsil AX. Inertsil CX has high ion exchange capacity and provides high retentivity and selectivity. Therefore, it is also suited for analyzing amino acids and nucleobases shown in Fig. and Fig. Figure : Comparison of lot-to-lot reproducibility with other brands CEC : 0. meq/g CEC : 0. meq/g Column Size : μm, 0.6 mm I.D. Eluent : 0. M CHCH (ph.0, HCH) Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 60 nm Injection vol. Sample : 0 μl :. Uracil (0.0 mg/ml). Thymine (0.0 mg/ml). Cytosine (0.0 mg/ml). Adenine (0.0 mg/ml) Inertsil CX Comitial Cation-Exchange column Figure : Biogenic Amine Analysis Figure : ucleoside Analysis CH H H H H H H H H. DL-DPA. Creatinine. DL-oradrenalin H H H H H H H H H. Uracil. Thymine. Guanine H H H H H H H. DL-Adrenaline. Dopamine hydrochoride 6. Tyramine H H 6 H H. Cytosine. Adenine Column : Inertsil CX ( μm, 0.6 mm I.D.) Eluent : 0. M KHP (ph 6.0, KHP) Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV nm Injection Vol. : 0 μl Sample :. DL-DPA (0.98 mg/ml). Creatinine (0. mg/ml). DL-oradrenalin (.0 mg/ml). DL-Adrenaline (.0 mg/ml). Dopamine hydrochloride (0.99 mg/ml) 6. Tyramine (.0 mg/ml) 0 0 Column : Inertsil CX ( μm, 0.6 mm I.D.) Eluent : 0. M HCH (ph.0, HCH) Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 60 nm Injection Vol. : 0 μl 0 Sample :. Uracil (0.0 mg/ml). Thymine (0.0 mg/ml). Guanine (0. mg/ml). Cytosine (0. mg/ml). Adenine (0. mg/ml) 080 HPLC Column Catalog

5 Inertsil CX Analytical : μm Length \ I.D Length \ I.D * End-fi ttings are /6 Waters-compatible. * For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm μm , , , , , Holder for Cartridge Guard Column E * End-fi ttings are /6 Waters-compatible. * For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC ormal Phase SEC Ion Exchange Application Specifi c Guard Preparative Capillary Applications Cat. o. Index HPLC Column Catalog 08

6 08 HPLC Column Catalog

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