groups I and II cations, amines, divalent transition metals

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1 IC Columns Introduction Alltech IC Column Specifications Column Composition ph Range Applications Anion Exchange Columns Allsep polymer-based anion ph 0 inorganic anions, weak and strong acid ions, exchanger, 7µm metal complexes, organic acids Novosep A- polymer-based anion ph inorganic anions and oxyhalides; ideal for exchanger, 5µm separation of seven common anions plus three oxyhalide anions in one run AN polymer-based anion exchanger, 9µm Anion/S silica-based anion exchanger, 0µm Anion/R polymer-based anion exchanger, 0µm PRP -X00 polymer-based anion exchanger, 0µm Cation Exchange Columns Universal Cation silica-based cation exchanger, 7µm Universal Cation silica-based cation HR exchanger, µm PRP -X00 polymer-based cation exchanger, 0µm Organic Acid Columns PRP -X00 polymer-based sulfonated cation exchanger Anion Exclusion polymer-based cation exchanger, 0µm Specialty Columns Surfactant C8 silica-based reversedphase, 5µm Surfactant C8 silica-based reversedphase, 5µm EPA Methods Optimized for Suppressed or Non-Suppressed 00.0, Part A both , suppressed , 7.0,.0 ph inorganic anions, weak and strong acid ions, both 00 organic acids ph 5.5 inorganic anions non-suppressed 0 ph inorganic anions, weak and strong acid ions non-suppressed 0 ph inorganic anions, weak and strong acid ions non-suppressed 0 ph 7 ph 7 ph groups I and II cations, amines, divalent transition metals groups I and II cations, amines, divalent transition metals; smaller particle size for improved peak resolution groups I and II cations (separate runs), amines, lanthanides Page 00.7 both both 0 non-suppressed 0 ph simple aliphatic carboxylic acids and alcohols non-suppressed 0 ph organic acids, weak acid anions non-suppressed 04 ph 7 short chain anionic surfactants non-suppressed 05 ph 7 long chain or aromatic anionic surfactants non-suppressed 05 IC Guard Kits Cross Reference Kit includes holder and three guard cartridges. Guard Reference Table Guard Cartridge Type All-Guard Kit Part No. Brand Column Type Alltech Allsep, Allsep A- GA- 809 Novosep A- GA- 809 Anion/R, Anion/S GA- 809 Universal Cation GC- 709 Universal Cation HR GC- 5 Anion Exclusion GIE- 87 Transition Metal C8 GRP- 8 Surfactant/R GRP- 8 Surfactant C8, C8 GRP- 8 Dionex IonPac AS4A-SC, AS7, AS, GA- 809 AS, AS4, AS, AS7 IonPac CS, CS0, CS GC- 8 IonPac CS, CS4, CS5 GC- 709 IonPac NS GRP- 8 IonPac AS, AS5 GIE- 87 Guard Reference Table (continued) Guard Cartridge Type All-Guard Kit Part No. Brand Column Type Waters IC-Pak -A GA- 809 IC-Pak -M/D GC- 709 Ion Exclusion GIE- 87 Hamilton PRP -X00 GA- 809 PRP -X00 GC- 8 PRP -X00 GIE- 87 Misc. Sarasep ANIONSEP AN GA- 809 Bio-Rad, Aminex, HPX-87 GIE- 87 Interaction ION-0 GA- 809 Interaction ION-0 GC- 8 Interaction ORH-80 GIE- 87 Metrohm Metrosep GA- 809 more info Cartridges and holders can also be purchased separately. See specific phase pages for replacement cartridge ordering information. 98

2 Alltech Anion Columns Allsep Anion IC Columns Resolves fluoride away from the water dip Meets requirements for U.S. EPA Method 00.0 part A The Allsep Anion Column is compatible with common IC mobile phases: carbonate, bicarbonate, p-hydroxybenzoic acid, phthalic acid, succinic acid, and sodium octane sulfonate. Use this column for performing EPA Method 00.0 Part A Determination of Inorganic Anions in Water per U.S. EPA guidelines. Resolves Fluoride Away from the Water Dip 0. Fluoride. Chloride. Nitrite 4. Bromide 5. Nitrate. Phosphate 7. Sulfate Min. Allsep Anion, 7µm, 4. x 00mm (Part No. 507) 0.85mM NaHCO/0.9mM NaCO.mL/min Suppressed Allsep Anion Specifications Methacrylate based w/quaternary ammonium functional groups Particle Size: 7µm Mobile Phase Limits: ph 0, 0 00% organic modifier Allsep Anion Columns Packing i.d. x Length PEEK Part No. SS Part No. Allsep Anion 4. x 50mm x 00mm x 50mm related product Save money with IC All-Guard Kits on page 98. Kit includes one holder and three cartridges. 99

3 Alltech and Transgenomic Anion Columns Novosep A- Anion IC Columns Separates seven inorganic anions and three oxyhalide anions in a single run Meets the requirements of U.S. EPA Methods 00.0, 00., 7.0, and.0 The Novosep A- Anion Column is ideal for the separation of 0 inorganic anions by suppressor-based ion chromatography using U.S. EPA Method 00.. The column can also be used to determine inorganic anions in drinking water per U.S. EPA Method 00.0 and trace bromate in drinking water using U.S. EPA Methods 7.0 and.0. Anions in High Ionic Strength Water by EPA Method Fluoride. Chlorite. Bromate 4. Chloride 5. Nitrite-N. Bromide 7. Chlorate 8. Dichloroacetate 9. Nitrate-N 0. Phosphate-P. Sulfate Transgenomic IC Sep AN IC Column Separates anions with carbonate/bicarbonate and hydroxide mobile phases The Transgenomic IC Sep AN Column is compatible with both suppressor-based and non-suppressed detection. A variety of mobile phases including sodium carbonate/ bicarbonate, sodium hydroxide, and borate/gluconate may be used. Separation of Sulfite and Sulfate Fluoride. Chloride. Nitrite 4. Bromide 5. Nitrate. Phosphate 7. Sulfite 8. Sulfate Min Min. Novosep A- Anion, 5µm, 4.0 x 50mm (Part No. 540).mM NaHCO 0.8mL/min Suppressed Transgenomic IC Sep AN, 9µm, 4. x 50mm (Part No. 840).8mM NaHCO,.8mM NaCO.0mL/min Suppressed Novosep A- Anion Specifications Polyvinyl alcohol-based w/quaternary ammonium functional groups Particle Size: 5µm Mobile Phase Limits: ph, 0 00% organic modifier, 0% modifier is practical upper limit Transgenomic IC Sep AN Specifications Poly(styrene-divinylbenzene) alkyl dimethyl ethanol ammonium functional group Particle Size: 9µm Exchange Capacity: 0.05meq/g Mobile Phase Limits: ph, No organic solvents Novosep A- Anion Column Packing i.d. x Length PEEK Part No. Novosep A- 4.0 x 50mm 540 Guards for Novosep A- Anion Columns GA- Anion Cartridges 808 All-Guard Cartridge Holder* ea 800 more applications To view our complete searchable chromatogram database visit /chromdb/ Transgenomic IC Sep AN Columns Packing i.d. x Length Part No. AN 4. x 50mm 840 Choose a guard column or integral guard discs to protect your IC Sep Column. Transgenomic IC Sep Guards Guard Column, 50 x 4.mm ea 845 Guard Column Guard-Disc

4 Alltech Anion Columns Anion/S IC Columns Silica-based for symmetrical peak shapes Separates inorganic and organic anions The Anion/S Columns are best suited for routine separations of chloride, bromide, nitrate, and sulfate. Not suitable for fluoride analysis Min. EDTA and Other Anions 884. Nitrite. EDTA. Bromide 4. Nitrate Anion/S, 0µm, 4. x 00mm (Part No. 90).5mM Sulfuric Acid.0mL/min UV at 0nm Anion/R IC Columns Polymer-based for broad ph stability Separates seven common inorganic anions Because of their high ph stability, these columns are ideal for the separation of anions using high ph mobile phases such as p-hydroxybenzoate, sodium hydroxide, and sodium carbonate/bicarbonate. The larger dimension columns (50mm and 50mm) are suitable for a high-resolution separation of the seven common inorganic anions. 45 Weak Acid Anions 597. Borate (5ppm). Silicate (0ppm). Formate (0ppm) 4. Sulfide (0ppm) 5. Chloride (0ppm). Cyanide (0ppm) Anion/S Specifications Silica w/quaternary ammonium ion exchanger Particle Size: 0µm Exchange Capacity: 0.5meq/g Mobile Phase Limits: ph 5.5, 0 00% organic modifier Min. Anion/R, 0µm, 4. x 50mm (Part No. 909) 4mM Sodium Hydroxide/ 0.mM Sodium Benzoate.5mL/min Anion/S Columns Packing i.d. x Length PEEK Part No. SS Part No. Anion/S 4. x 00mm x 50mm 900 Anion/R Specifications Poly(styrene-divinylbenzene) Trimethylammonium Particle Size: 0µm Exchange Capacity: 0.9 ± 0.0meq/g Mobile Phase Limits: ph, 0 00% organic modifier related product Save money with IC All-Guard Kits on page 98. Kit includes one holder and three cartridges. Anion/R Columns Packing i.d. x Length PEEK Part No. SS Part No. Anion/R 4. x 00mm x 50mm x 00mm x 50mm 904 Guards for Anion/S and Anion/R Columns GA- Anion Cartridges 808 All-Guard Cartridge Holder* ea 800 0

5 Alltech Cation Columns Universal Cation and Universal Cation HR IC Columns Separate Groups I and II cations in one isocratic run Separate transition metals without post-column reaction New Universal Cation HR µm particle size for improved efficiency Compatible with a variety of mobile phases including complexing acids (citric acid, tartaric acid, oxalic acid), mineral acids (nitric acid, hydrochloric acid, sulfuric acid) and non-complexing organic acids (methanesulfonic acid). 4. Lithium (0.ppm). Sodium (.5ppm). Ammonium (.5ppm) 4. Potassium (.5ppm) Metal Ions Min Nickel (.5ppm). Zinc (.5ppm) 7. Cobalt (.5ppm) 8. Magnesium (.0ppm) 9. Calcium (.0ppm) Min. 4 5 Cesium and Strontium Universal Cation, 7µm, 4. x 00mm (Part No. 70) mm Methanesulfonic Acid.0mL/min Lithium (0.ppm). Sodium (0.90ppm). Ammonium (0.90ppm) 4. Potassium (.5ppm) 5. Cesium (0.8ppm). Magnesium (.ppm) 7. Calcium (.ppm) 8. Strontium (.ppm) Universal Cation, 7µm, 4. x 00mm (Part No. 70) mm Tartaric Acid/mM Oxalic Acid.0mL/min Universal Cation Specifications Silica coated with polybutadiene/maleic acid copolymer Particle Size: µm and 7µm, spherical Mobile Phase Limits: ph 7, 0 00% organic modifier 4 Rapid Analysis of Monovalent and Divalent Cations 5 0. Lithium. Sodium. Ammonium 4. Potassium 5. Magnesium. Calcium Universal Cation Columns Packing i.d. x Length PEEK Part No. SS Part No. 7µm 4. x 00mm µm HR 4. x 00mm x 5mm 09 technical assistance Contact Tech Support: Phone: (North America) contact.alltech@grace.com Online: Min. Universal Cation HR, µm, 7.0 x 5mm (Part No. 09) mm Methanesulfonic Acid.5mL/min more applications To view our complete searchable chromatogram database visit /chromdb/ Guards for Universal Cation Columns GC- Universal Cation 700 GC- Universal Cation HR 0 All-Guard Cartridge Holder* ea 800 related product Save money with IC All-Guard Kits on page 98. Kit includes one holder and three cartridges. 0

6 Hamilton Anion, Cation, and Organic Acid Columns PRP -X00 Anion Columns High ph stability makes this column ideal for the separation of weak anions. PRP TM -X00 provides excellent resolution for the seven most frequently analyzed inorganic anions. For non-suppressed analyses only. PRP -X00 Cation Analysis Columns For separation of the alkali metal and alkaline earth cations in separate runs. PRP -X00 Organic Acid Columns For separation of simple aliphatic carboxylic acids and alcohols. Inorganic anions are not retained. EDTA in Eye Drops 808. CuEDTA Hamilton PRP -X00 Specifications Trimethylammonium psdvb copolymer Particle Size: 0µm, spherical Exchange Capacity: 0.9meq/g Mobile Phase Limits: ph, 0 00% organic modifier Hamilton PRP -X00 Specifications Sulfonated psdvb copolymer Particle Size: 0µm, spherical Exchange Capacity: 0.5meq/g Mobile Phase Limits: ph, 0 00% organic modifier Hamilton PRP -X00 Specifications Sulfonated psdvb in H+ form Particle Size: µm and 7µm Mobile Phase Limits: ph, 0 00% organic modifier Alkali Metals 504. Lithium. Sodium. Ammonium 4. Potassium 5. Cesium Min. PRP -X00, 0µm, 4. x 50mm (Part No. 7944) 0.00N Sulfuric Acid ml/min UV at 54nm Min. PRP -X00, 0µm, 4. x 50mm (Part No. 7944) 0.004N HNO in 0% MeOH ml/min Hamilton PRP -X00 Columns Packing i.d. x Length Part No. PRP -X00. x 50mm x 50mm x 00mm x 50mm x 50mm 794 Hamilton PRP -X00 Columns Particle Size i.d. x Length Part No. PRP -X00 4. x 50mm x 50mm 7944 Hamilton PRP -X00 Guard Kits and Cartridges Guard Column Starter Kit ea PRP -X00, Holder, Cartridges Replacement Guard Cartridges (Requires Hamilton Guard Holder) PRP -X00, 5 x.mm Hamilton PRP -X00 Guard Kits and Cartridges Replacement Guard Cartridges (Requires Hamilton Guard Holder) PRP -X00, 5 x.mm Hamilton PRP -X00 Columns Particle Size i.d. x Length Part No. PRP -X00, 7µm 4. x 50mm x 50mm 7945 Hamilton PRP -X00 Guard Kits and Cartridges Replacement Guard Cartridges (Requires Hamilton Guard Holder) PRP -X00, 5 x.mm

7 Alltech Anion Exclusion Columns Separate organic acids and weak acid anions Polymer-based for broad ph stability Economical choice for small inorganics Anion Exclusion Columns separate organic acids and weakly ionized anions by an anion exclusion mechanism. Dilute mineral acids are the typical mobile phases for the separation. Acetonitrile may be used as an organic modifier to decrease the retention of hydrophobic compounds. Organic Acid Standards 07. Oxalic Acid. Maleic Acid. Malic Acid 4. Succinic Acid 5. Formic Acid. Acetic Acid Min. Anion Exclusion, 0µm, 7.5 x 00mm (Part No. 90).5mM Sulfuric Acid 0.4mL/min Anion Exclusion Specifications Highly sulfonated poly (styrene-divinylbenzene) cation exchanger Particle Size: 0µm Mobile Phase Limits: <0% acetonitrile, no methanol <5% isopropyl or ethyl alcohols Anion Exclusion Columns Packing i.d. x Length SS Part No. Anion Exclusion, 0µm 7.8 x 00mm x 00mm 900 Anion Exclusion All-Guard Cartridges Anion Exclusion GIE- 88 All-Guard Cartridge Holder* ea 800 related product Save money with IC All-Guard Kits on page 98. Kit includes one holder and three cartridges. 04

8 Alltech Surfactant Columns Surfactant/C IC Columns For non-suppressed analyses Silica-based for symmetrical peak shapes Choose Surfactant C8 for short chain anionic surfactants such as alkyl sulfates and alkyl sulfonates. Choose Surfactant C8 for long chain or aromatic surfactants such as xylene sulfonate and dodecyl benzene sulfonate. Alkyl Sulfates Octyl Sulfate. Decyl Sulfate. Dodecyl Sulfate Min. Surfactant C8, 5µm, 4. x 50mm (Part No. 907) 0.0g/L Ammonium Acetate in 40% Methanol.mL/min Surfactant/C Specifications Silica-based C8 or C8 Particle Size: 5µm Mobile Phase Limits: ph 7, 0 00% organic modifier Surfactant/C Columns Packing i.d. x Length SS Part No. C8, 5µm 4. x 50mm 909 C8, 5µm 4. x 50mm 909 Guards for Surfactant/C Columns GRP- Revised Phase 89 All-Guard Cartridge Holder* ea

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