IC Columns Introduction

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1 IC Columns Introduction Alltech IC Column Specifications Column Composition ph Range Applications Anion Exchange Columns Allsep polymer-based anion ph 0 inorganic anions, weak and strong acid ions, exchanger, μm metal complexes, organic acids Allsep A- polymer-based anion ph inorganic anions, organic acids, suitable for exchanger, μm both weak and strong anions in a single run Novosep A- polymer-based anion ph inorganic anions and oxyhalides; ideal for exchanger, 5μm separation of seven common anions plus three oxyhalide anions in one run AN polymer-based anion exchanger, 9μm Anion/S silica-based anion exchanger, 0μm Anion/R polymer-based anion exchanger, 0μm PRP -X00 polymer-based anion exchanger, 0μm Cation Exchange Columns Universal Cation silica-based cation exchanger, μm Universal Cation silica-based cation HR exchanger, μm PRP -X00 polymer-based cation exchanger, 0μm Organic Acid Columns PRP -X00 polymer-based sulfonated cation exchanger Anion Exclusion polymer-based cation exchanger, 0μm Specialty Columns Transition Metal silica-based reversedphase, μm and μm Surfactant/R Surfactant C Surfactant C polymer-based reversed-phase, μm silica-based reversedphase, 5μm silica-based reversedphase, 5μm EPA Methods Optimized for Suppressed or Non-Suppressed 00.0, Part A both , both 99 Part A 00.0, suppressed ,.0,.0 ph inorganic anions, weak and strong acid ions, both 00 organic acids ph 5.5 inorganic anions non-suppressed 0 ph inorganic anions, weak and strong acid ions non-suppressed 0 ph inorganic anions, weak and strong acid ions non-suppressed 0 ph ph ph groups I and II cations, amines, divalent transition metals groups I and II cations, amines, divalent transition metals; smaller particle size for improved peak resolution groups I and II cations (separate runs), amines, lanthanides Page 00. both both 0 non-suppressed 0 ph simple aliphatic carboxylic acids and alcohols non-suppressed 0 ph organic acids, weak acid anions non-suppressed 04 ph high capacity, separates divalent transition metals and metal-cyano complexes more selectively than a cation exchange column non-suppressed 04 ph 4 anionic and cationic surfactants; separate both 05 short and long chain surfactants in one gradient run ph short chain anionic surfactants non-suppressed 05 ph long chain or aromatic anionic surfactants non-suppressed 05 IC Guard Kits Cross Reference Kit includes holder and three guard cartridges. Guard Reference Table Guard Cartridge Type All-Guard Kit Part No. Brand Column Type Alltech Allsep, Allsep A- GA- 09 Novosep A- GA- 09 Anion/R, Anion/S GA- 09 Universal Cation GC- 09 Universal Cation HR GC- 5 Anion Exclusion GIE- Transition Metal C GRP- Surfactant/R GRP- Surfactant C, C GRP- Dionex IonPac AS4A-SC, AS, AS, GA- 09 AS, AS4, AS, AS IonPac CS, CS0, CS GC- IonPac CS, CS4, CS5 GC- 09 IonPac NS GRP- IonPac AS, AS5 GIE- Guard Reference Table (continued) Guard Cartridge Type All-Guard Kit Part No. Brand Column Type Waters IC-Pak -A GA- 09 IC-Pak -M/D GC- 09 Ion Exclusion GIE- Hamilton PRP -X00 GA- 09 PRP -X00 GC- PRP -X00 GIE- Misc. Sarasep ANIONSEP AN GA- 09 Bio-Rad, Aminex, HPX- GIE- Interaction ION-0 GA- 09 Interaction ION-0 GC- Interaction ORH-0 GIE- Metrohm Metrosep GA- 09 more info Cartridges and holders can also be purchased separately. See specific phase pages for replacement cartridge ordering information. 9

2 Alltech Anion Columns Allsep Anion IC Columns Resolves fluoride away from the water dip Meets requirements for U.S. EPA Method 00.0 part A The Allsep Anion Column is compatible with common IC mobile phases: carbonate, bicarbonate, p-hydroxybenzoic acid, phthalic acid, succinic acid, and sodium octane sulfonate. Use this column for performing EPA Method 00.0 Part A Determination of Inorganic Anions in Water per U.S. EPA guidelines. 4 Resolves Fluoride Away from the Water Dip 5 0. Fluoride. Chloride. Nitrite 4. Bromide 5. Nitrate. Phosphate. Sulfate Allsep A- Anion IC Columns Separates both weak and strong acid anions in one run Resolves formate away from fluoride and chloride The Allsep A- Anion Column separates both weakly retained organic and strongly retained inorganic anions in one run. The high capacity Allsep A- Anion Column is also suitable for the separation of chlorite and chlorate. 4 5 Organic and Inorganic Anions Fluoride (.0ppm). Acetate (.0ppm). Formate (.0ppm) 4. Chlorite (.0ppm) 5. Chloride (.0ppm). Nitrite (.0ppm). Bromide (.0ppm). Chlorate (5.0ppm) 9. Nitrate (.0ppm) 0. Phosphate (.0ppm). Sulfate (.0ppm) Min. 0 4 Min. Allsep Anion, μm, 4. x 00mm (Part No. 50) 0.5mM NaHCO/0.9mM NaCO.mL/min Suppressed Allsep A- Anion, μm, 4. x 00mm (Part No. 59).mM NaHCO/.mM NaCO.0mL/min Suppressed Allsep Anion Specifications Methacrylate based w/quaternary ammonium functional groups μm Mobile Phase Limits: ph 0, 0 00% organic modifier Allsep A- Anion Specifications Methacrylate based w/quaternary alkanol amine functional groups μm Mobile Phase Limits: ph, 0 00% organic modifier Allsep Anion Columns Allsep Anion 4. x 0mm x 50mm x 00mm x 50mm Allsep A- Anion Columns Allsep A- 4. x 50mm x 00mm 59 5 Guards for Allsep and Allsep A- Columns GA- Anion Cartridges 0 All-Guard Cartridge Holder* ea 00 99

3 Alltech Anion Columns Novosep A- Anion IC Columns Separates seven inorganic anions and three oxyhalide anions in a single run Meets the requirements of U.S. EPA Methods 00.0, 00.,.0, and.0 The Novosep A- Anion Column is ideal for the separation of 0 inorganic anions by suppressor-based ion chromatography using U.S. EPA Method 00.. The column can also be used to determine inorganic anions in drinking water per U.S. EPA Method 00.0 and trace bromate in drinking water using U.S. EPA Methods.0 and.0. Anions in High Ionic Strength Water by EPA Method Fluoride. Chlorite. Bromate 4. Chloride 5. Nitrite-N. Bromide. Chlorate. Dichloroacetate 9. Nitrate-N 0. Phosphate-P. Sulfate IC Sep AN IC Column Separates anions with carbonate/bicarbonate and hydroxide mobile phases The IC Sep AN Column is compatible with both suppressor-based and non-suppressed detection. A variety of mobile phases including sodium carbonate/bicarbonate, sodium hydroxide, and borate/gluconate may be used. Separation of Sulfite and Sulfate 4 5. Fluoride. Chloride. Nitrite 4. Bromide 5. Nitrate. Phosphate. Sulfite. Sulfate Min Min. Novosep A- Anion, 5μm, 4.0 x 50mm (Part No. 540).mM NaHCO 0.mL/min Suppressed IC Sep AN, 9μm, 4. x 50mm (Part No. 40).mM NaHCO,.mM NaCO.0mL/min Suppressed Novosep A- Anion Specifications Polyvinyl alcohol-based w/quaternary ammonium functional groups 5μm Mobile Phase Limits: ph, 0 00% organic modifier, 0% modifier is practical upper limit IC Sep AN Specifications Poly(styrene-divinylbenzene) alkyl dimethyl ethanol ammonium functional group 9μm Exchange Capacity: 0.05meq/g Mobile Phase Limits: ph, No organic solvents Novosep A- Anion Column Packing i.d. x Length PEEK Part No. Novosep A- 4.0 x 50mm 540 Guards for Novosep A- Anion Columns GA- Anion Cartridges 0 All-Guard Cartridge Holder* ea 00 more applications To view our complete searchable chromatogram database visit /chromdb/ IC Sep AN Columns Packing i.d. x Length Part No. AN 4. x 50mm 40 Choose a guard column or integral guard discs to protect your IC Sep Column. IC Sep Guards Guard Column, 50 x 4.mm ea 45 Guard Column Guard-Disc

4 Alltech Anion Columns Anion/S IC Columns Silica-based for symmetrical peak shapes Separates inorganic and organic anions The Anion/S Columns are best suited for routine separations of chloride, bromide, nitrate, and sulfate. Not suitable for fluoride analysis Min. EDTA and Other Anions 4. Nitrite. EDTA. Nitrate 4. Bromide Anion/S, 0μm, 4. x 00mm (Part No. 90).5mM Sulfuric Acid.0mL/min UV at 0nm Anion/R IC Columns Polymer-based for broad ph stability Separates seven common inorganic anions Because of their high ph stability, these columns are ideal for the separation of anions using high ph mobile phases such as p-hydroxybenzoate, sodium hydroxide, and sodium carbonate/bicarbonate. The larger dimension columns (50mm and 50mm) are suitable for a high-resolution separation of the seven common inorganic anions. 45 Weak Acid Anions 59. Borate (5ppm). Silicate (0ppm). Formate (0ppm) 4. Sulfide (0ppm) 5. Chloride (0ppm). Cyanide (0ppm) Anion/S Specifications Exchange Capacity: Mobile Phase Limits: Silica w/quaternary ammonium ion exchanger 0μm 0.5meq/g ph 5.5, 0 00% organic modifier 0 9 Min. Anion/R, 0μm, 4. x 50mm (Part No. 909) 4mM Sodium Hydroxide/ 0.mM Sodium Benzoate.5mL/min Anion/S Columns Anion/S 4. x 00mm x 50mm Anion/R Specifications Exchange Capacity: Mobile Phase Limits: Poly(styrene-divinylbenzene) Trimethylammonium 0μm 0.9 ± 0.0meq/g ph, 0 00% organic modifier Anion/R Columns Anion/R 4. x 00mm x 50mm x 00mm x 50mm 904 Guards for Anion/S and Anion/R Columns GA- Anion Cartridges 0 All-Guard Cartridge Holder* ea 00 0

5 Alltech Cation Columns Universal Cation and Universal Cation HR IC Columns Separate Groups I and II cations in one isocratic run Separate transition metals without post-column reaction New Universal Cation HR μm particle size for improved efficiency Compatible with a variety of mobile phases including complexing acids (citric acid, tartaric acid, oxalic acid), mineral acids (nitric acid, hydrochloric acid, sulfuric acid) and non-complexing organic acids (methanesulfonic acid). 4. Lithium (0.ppm). Sodium (.5ppm). Ammonium (.5ppm) 4. Potassium (.5ppm) 5 Metal Ions Min Nickel (.5ppm). Zinc (.5ppm). Cobalt (.5ppm). Magnesium (.0ppm) 9. Calcium (.0ppm) Min. 4 5 Cesium and Strontium Universal Cation, μm, 4. x 00mm (Part No. 0) mm Methanesulfonic Acid.0mL/min 05. Lithium (0.ppm). Sodium (0.90ppm). Ammonium (0.90ppm) 4. Potassium (.5ppm) 5. Cesium (0.ppm). Magnesium (.ppm). Calcium (.ppm). Strontium (.ppm) Universal Cation, μm, 4. x 00mm (Part No. 0) mm Tartaric Acid/mM Oxalic Acid.0mL/min Universal Cation Specifications Silica coated with polybutadiene/maleic acid copolymer μm and μm, spherical Mobile Phase Limits: ph, 0 00% organic modifier Rapid Analysis of Monovalent and Divalent Cations 0. Lithium. Sodium. Ammonium 4. Potassium 5. Magnesium. Calcium Universal Cation Columns μm 4. x 00mm 0 00 μm HR 4. x 00mm 00.0 x 5mm 09 technical assistance Contact Tech Support: Phone: (North America) contact.alltech@grace.com Online: Universal Cation HR, μm,.0 x 5mm (Part No. 09) mm Methanesulfonic Acid.5mL/min more applications To view our complete searchable chromatogram database visit /chromdb/ Guards for Universal Cation Columns GC- Universal Cation 00 GC- Universal Cation HR 0 All-Guard Cartridge Holder* ea 00 0

6 Hamilton Anion, Cation, and Organic Acid Columns PRP -X00 Anion Columns High ph stability makes this column ideal for the separation of weak anions. PRP TM -X00 provides excellent resolution for the seven most frequently analyzed inorganic anions. For non-suppressed analyses only. PRP -X00 Cation Analysis Columns For separation of the alkali metal and alkaline earth cations in separate runs. PRP -X00 Organic Acid Columns For separation of simple aliphatic carboxylic acids and alcohols. Inorganic anions are not retained. EDTA in Eye Drops 0. CuEDTA Hamilton PRP -X00 Specifications Trimethylammonium psdvb copolymer 0μm, spherical Exchange Capacity: 0.9meq/g Mobile Phase Limits: ph, 0 00% organic modifier Hamilton PRP -X00 Specifications Sulfonated psdvb copolymer 0μm, spherical Exchange Capacity: 0.5meq/g Mobile Phase Limits: ph, 0 00% organic modifier Hamilton PRP -X00 Specifications Sulfonated psdvb in H+ form μm and μm Mobile Phase Limits: ph, 0 00% organic modifier Alkali Metals 504. Lithium. Sodium. Ammonium 4. Potassium 5. Cesium Min. PRP -X00, 0μm, 4. x 50mm (Part No. 944) 0.00N Sulfuric Acid ml/min UV at 54nm Min. PRP -X00, 0μm, 4. x 50mm (Part No. 944) 0.004N HNO in 0% MeOH ml/min Hamilton PRP -X00 Columns Packing i.d. x Length Part No. PRP -X00. x 50mm 94. x 50mm x 00mm x 50mm x 50mm 94 Hamilton PRP -X00 Columns Particle Size i.d. x Length Part No. PRP -X00. x 50mm 994. x 50mm x 50mm x 50mm 944 Hamilton PRP -X00 Columns Particle Size i.d. x Length Part No. PRP -X00, μm 4. x 50mm 99 PRP -X00, μm 4. x 50mm x 50mm x 50mm 945 Hamilton PRP -X00 Guard Kits and Cartridges Guard Column Starter Kit ea 944 PRP -X00, Holder, Cartridges Replacement Guard Cartridges (Requires Hamilton Guard Holder) PRP -X00, 5 x.mm Hamilton PRP -X00 Guard Kits and Cartridges Guard Column Starter Kit ea 945 PRP -X00, Holder, Cartridges Replacement Guard Cartridges (Requires Hamilton Guard Holder) PRP -X00, 5 x.mm Hamilton PRP -X00 Guard Kits and Cartridges Guard Column Starter Kit ea 940 PRP -X00, Holder, Cartridges Replacement Guard Cartridges (Requires Hamilton Guard Holder) PRP -X00, 5 x.mm

7 Alltech Anion Exclusion Columns Separate organic acids and weak acid anions Polymer-based for broad ph stability Economical choice for small inorganics Anion Exclusion Columns separate organic acids and weakly ionized anions by an anion exclusion mechanism. Dilute mineral acids are the typical mobile phases for the separation. Acetonitrile may be used as an organic modifier to decrease the retention of hydrophobic compounds. Organic Acid Standards 0. Oxalic Acid. Maleic Acid. Malic Acid 4. Succinic Acid 5. Formic Acid. Acetic Acid Alltech Transition Metal Columns Separate divalent transition metals and metal-cyano complexes more selectively than a cation exchange column To separate divalent transition metals, use conductivity detection for concentrations as low as ppm, or UV detection with post-column reaction for ppb-level concentrations To separate metal-cyano complexes, use the Alltech low UV IPC A (Part No. 549) as the ion-pair reagent, and methanol or acetonitrile as the organic modifier. Metal-Cyano Complexes Min.. Fe(CN) 4-. Ag(CN) -. Fe(CN) - 4. Ni(CN)4-5. Au(CN) - Anion Exclusion, 0μm,.5 x 00mm (Part No. 90).5mM Sulfuric Acid 0.4mL/min Anion Exclusion Specifications Highly sulfonated poly (styrene-divinylbenzene) cation exchanger 0μm Mobile Phase Limits: <0% acetonitrile, no methanol <5% isopropyl or ethyl alcohols Anion Exclusion Columns Anion Exclusion, 0μm.5 x 00mm x 00mm 90. x 00mm 90. x 00mm 900 Anion Exclusion All-Guard Cartridges Anion Exclusion GIE- All-Guard Cartridge Holder* ea 00 Transition Metal C, μm, 4. x 50mm (Part No. 90) 0.005M Tetrabutylammonium Sulfate (Preformulated Alltech Low UV IPC A, Part No. 549) in 0% Acetonitrile 0.mL/min UV at 4nm Transition Metal Specifications Silica-based with C bonded phase (0% Carbon) μm and μm, spherical Mobile Phase Limits: ph Transition Metal Columns Packing i.d. x Length PEEK Part No. C, μm 4. x 00mm 904 C, μm 4. x 50mm 90 Transition Metal All-Guard Cartridges GRP- Revised Phase 9 All-Guard Cartridge Holder* ea 00 04

8 Alltech Surfactant Columns Surfactant/R IC Columns Separate anionic and cationic surfactants Separate short and long chain surfactants in one gradient run The Surfactant/R Column separates anionic and cationic surfactants by ion-pair chromatography along with suppressed conductivity detection. This column can also be used for other reversed-phase applications. Short and Long Chain Cationic Surfactants Tetramethyl Ammonium (0ppm). Tetraethyl Ammonium (0ppm). Tetrapropyl Ammonium (0ppm) 4. Tetrabutyl Ammonium (0ppm) 5. Dodecyltrimethyl Ammonium (0ppm). Cetyltrimethyl Ammonium (40ppm). Myristyltrimethyl Ammonium (50ppm) Surfactant/C IC Columns For non-suppressed analyses Silica-based for symmetrical peak shapes Choose Surfactant C for short chain anionic surfactants such as alkyl sulfates and alkyl sulfonates. Choose Surfactant C for long chain or aromatic surfactants such as xylene sulfonate and dodecyl benzene sulfonate. Alkyl Sulfates 599. Octyl Sulfate. Decyl Sulfate. Dodecyl Sulfate Min. Surfactant/R Column, μm, 4. x 50mm (Part No. 50) A: mm Nonafluoropentanoic Acid B: 00% Acetonitrile Gradient: Time: %B: mL/min Suppressed Min. Surfactant/R Specifications Polydivinylbenzene (DVB) based resin μm Mobile Phase Limits: ph 4, 0 00% organic modifier Surfactant C, 5μm, 4. x 50mm (Part No. 90) 0.0g/L Ammonium Acetate in 40% Methanol.mL/min Surfactant/R Columns Surfactant/R, μm 4. x 50mm Guards for Surfactant/R Columns GRP- Revised Phase 4 All-Guard Cartridge Holder* ea 00 more applications To view our complete searchable chromatogram database visit /chromdb/ Surfactant/C Specifications Silica-based C or C 5μm Mobile Phase Limits: ph, 0 00% organic modifier Surfactant/C Columns C, 5μm 4. x 50mm C, 5μm 4. x 50mm Guards for Surfactant/C Columns GRP- Revised Phase 9 All-Guard Cartridge Holder* ea 00 05

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