Method Development Kits

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1 Method Development Kits sales representative for ordering information or contact...77

2 Method Development Kits First column choice for Pharma Development USP L C Method Development Kits Maximize retention for highly polar compounds C Rapid elution of samples ID x Length (in mm) USP L C Method Development Kits Includes C, AQ-C, Swift C μm μm μm. x IN-MDK-C- IN-MDK-C- -. x IN-MDK-C- IN-MDK-C- - AQ-C Swift C. x - - IN-MDK-C-. x - IN-MDK-C- - First column choice for Drug Discovery Alternative Selectivity Method Development Kits Alternate retention mode for highly polar compounds Swift C Change in Selectivity Alternative Selectivity Method Development Kits Includes Swift C, Phenyl, Amide (HILIC) ID x Length (in mm) μm μm. x IN-MDK-AS- -. x IN-MDK-AS- -. x IN-MDK-AS- - Amide (HILIC) Phenyl. x IN-MDK-AS- -. x IN-MDK-AS- IN-MDK-AS-. x - IN-MDK-AS- Design your own Kit C Phenyl Design your own Method Development Kit Maximize retention for highly polar compounds Amide (HILIC) AQ-C Alternate retention mode for highly polar compounds Swift C Change in Selectivity Rapid elution of samples Select your phases ( different phases) Select your dimension (must all be the same dimension) Select your particle sizes Contact your sales representative for a quote and get % discount on your custom kit. sales representative for ordering information or contact...77

3 μm (UHPLC Guard Set) + cartridges ( μm) C Guard s μm + cartridges ( μm) μm + cartridges ( μm). x - - IN-9 IN-9 IN-999 IN-99.,.. x IN- IN x IN-99 IN- IN-9 IN-9 IN-999 IN-99.. x - - IN-9 IN-9 IN-999 IN-99.,. μm (UHPLC Guard Set) + cartridges ( μm) AQ-C Guard s μm + cartridges ( μm) μm + cartridges ( μm). x - - IN-99 IN-99 IN-9 IN-9.,.. x IN-99 IN x IN-999 IN-99 IN-999 IN-99 IN-97 IN-9.. x - - IN-997 IN-99 IN-9 IN-9.,. μm (UHPLC Guard Set) + cartridges ( μm) Swift C Guard s μm + cartridges ( μm) μm + cartridges ( μm). x - - IN- IN- IN- IN-7.,.. x IN- IN x IN- IN- IN-9 IN-97 IN- IN-.. x - - IN-9 IN-9 IN- IN-.,. μm (UHPLC Guard Set) + cartridges ( μm) Phenyl Guard s μm + cartridges ( μm) μm + cartridges ( μm). x - - IN- IN-9 IN-9 IN-.,.. x IN- IN x IN- IN- IN- IN- IN- IN-.. x - - IN- IN- IN- IN-.,. μm + cartridges Amide Guard s ( μm) μm + cartridges ( μm). x IN- IN-7 IN-7 IN-7.,.. x ,.. x IN- IN- IN-7 IN-77.. x IN- IN- IN-7 IN-7.,. Dedicated Holder PEEK Guide Packing Guard E Tubing UHPLC Guard sales representative for ordering information or contact...77

4 Method Development Specifications Phases USP Designation Method Development Specifications Particle Size(s) Carbon Load (%) Pore Size Surface Area (m / g) ph Range (From - C) C L,, - Max. perating Temp. C (ph -7) C (ph 7-) MDK # MDK # AQ-C L.9,, - C (ph -7) C (ph 7-) Swift C L.9,, 9 - C (ph -7) C (ph 7-) Phenyl L,, -7. C (ph -7.) Amide (HILIC) L.9,, 9 - C (ph -7) C (ph 7-) Strategies for Comprehensive Method Development using Multiple Stationary Phases Wide ph Compatibility Maximize your Method Development Efficiency C AQ-C Swift Phenyl Screening Gradients -9% CH CN, low ph -9% CH H, low ph -9% CH CN, mid ph -9% CH H, mid ph -9% CH CN, high ph -9% CH H, high ph Select phase, ph, solvent ptimize t G, T, ph ph Amide (HILIC) Screening Gradients 9-% CH CN, NH HC, low ph 9-% CH CN, NH HC, mid ph 9-% CH CN, NH HC, high ph 9-% CH CN, NH CH C, low ph 9-% CH CN, NH CH C, mid ph 9-% CH CN, NH CH C, high ph Select phase, ph, solvent ptimize t G, T, ph ph ph 7 Cond Colu : Elue C ( µm,. mm I.D.) Eluent : A) CHCN B) mm Phosphate, Borate, Acetate Buffer A / Flow B = / 7, v / v Flow Rate :. Col. ml / min Col. Temp. : Dete C Detection : UV Sam nm Sample :.,-Dimethylpyridine. Aniline. Benzoic acid. Acetophenone.,-Dimethylbenzoic acid ph 9 Time(min) sales representative for ordering information or contact...77

5 C Wide ph Compatibility with Long Lifetime : ES Gel : μm, μm, μm : m /g : Å :. ml/g Bonded Phase : ctadecyl Groups End-capping : Complete Carbon Loading : % USP Code : L ph Range : - % Initial N Tailing developed after 7 hours on XTerra MS C and Gemini-NX C. C XTerra MS C (Waters) Gemini-NX C (Phenomenex) Purging :. mm I.D. mm μm Eluent : A) mm Triethylamine (ph.) B) CH H A / B = 7 /. V/V. Flow Rate :. ml / min Col. Temp. : C Analytical :. mm I.D. mm μm Eluent :CH CN / H ( / ) Flow Rate :. ml / min Col. Temp. : C Detection :UV nm (Naphthalene) Hours in mm TEA (ph ) / CH H = 7 /, at C As shown in the experiment above, C maintained high efficiency and peak shape for hrs C inherits the advantages of all the current Inertsil HPLC columns (e.g., extremely low operating back pressure, superior inertness to typically any analytes, high efficiency and compatibility with a wide range of solvents), but now can be used for wide ph analysis with consistent performance from column to column and lot to lot. AQ-C : ES Gel :.9 μm, μm, μm : m /g : Å :. ml/g Bonded Phase : ctadecyl Groups End-capping : Complete Carbon Loading : % USP Code : L ph Range : - Retention Factor of acidic, basic and neutral compounds using % water mobile phase Aquasil C Atlantis T Inertsil DS- Inertsil DS-SP CAPCELL PAK C-AQ Synergi Fusion RP XSelect CSH C Retention Properties of AQ-C I nerts ustain AQ- C Retention Factor of amylbenzene using methanol/water= / Inertsil DS- C Remarks:. The horizontal and vertical retention factor values were calculated with reference to the retention factor values obtained from AQ-C.. The horizontal values represents the retention factor of amylbenzene using methanol/water= /.. The vertical values represents the retention factor of acidic, basic and neutral compounds using % water mobile phase. AQ-C columns are designed to achieve strong retention for highly polar compounds, which is the most challenging goal in developing reversed phase methods. The optimization of bonding the C groups at equal distance to the silica gel enables AQ-C to offer significant retention for highly polar compounds even under water rich mobile phases. sales representative for ordering information or contact...77

6 Swift C HPLC s Swift C Rapid Elution of Samples in Isocratic Methods The retentivity of Swift C is weaker even comparing with a conventional C column, which makes it ideal for methods requiring maximum throughput and efficiency. Eluent : ES Gel :.9 μm, μm, μm : m/g : Å :. ml/g Bonded Phase : ctadecyl Groups End-capping : Complete Carbon Loading : 9. % USP Code : L ph Range : - Size Flow Rate Col. Temp. Detection Sample Swift C : A) CHH B) H A/B = /, v/v : μm, x. mm I.D. :. ml/min : : UV nm. Uracil. Toluene. Ethylbenzene. Propylbenzene. n-butylbenzene. n-amylbenzene C Inertil DS-SP Inertil DS- C Inertsil DS- Sample No., n-amylbenzene was used to determine the hydrophobic property of columns. As shown above, Swift C delivers rapid elution of samples in isocratic methods. Swift C maintains the same extreme inertness, wide ph range and provides rapid separations with symmetric peaks. The optimization of surface area, pore size and chemical bonding delivers superior peak shapes. Swift C is also ideal for LC/MS/MS applications which delivers highly sensitive results and enables MS compatible buffers to be used due to the extremely inert silica gel. Phenyl Comparison of Selectivity between C, Phenyl and ther Brand Phenyl Phenyl C ther Brand Phenyl, : ES Gel : μm, μm, μm : m/g : Å :. ml/g Bonded Phase : Phenyl Groups End-capping : None Carbon Loading : % USP Code : L ph Range : -7. Flow Rate Col. Temp. Detection, Size Eluent μm, x. mm I.D. A) CHH B) H A / B = 7 /, v / v. ml / min UV nm. o-terphenyl. Amylbenzene (Steric Structure). Butylbenzene. Triphenylene (Planar Structure) Phenyl has an extremely unique reverse phase characteristic that is critical to resolving compounds that could not be separated on a C or C phase. Phenyl provides not only π-π interactions, but also hydrogen bonding secondary Phenyl Brands Phenyl A ther Brand Phenyl B interactions, which results in retaining polar compounds at the same time.ther As the phenyl groups are directly bonded to the silica gel, Phenyl is compatible with the analysis of structural isomers due to it s high stereo-selectivity while other,alkyl phenyl, type columns fails to separate. Not Separated Size Eluent μm, x. mm I.D. A) CHH B) H A / B = /, v / v. ml / min UV nm H Not Separated H H For more information please visit - Please contact your local C H sales representative for ordering information or contact...77 Col. Temp. Flow Rate Detection C H.o-Cresol.m-Cresol C H.p-Cresol

7 Amide Retention Mechanism in HILIC Mode Mobile Phase ACN H : ES Gel :.9 μm, μm, μm : m /g : Å :. ml/g Bonded Phase : Carbamoyl Groups End-capping : Yes Carbon Loading : 9. % USP Code : L ph Range : - Hydration Layer H Analyte H - H Si H Hydrogen Bonding Analyte + H - H Amide is a HILIC (Hydrophilic-Interaction Chromatography) column for enhanced retention of extremely polar compounds. ffering the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. Amide C. Uracil. Pyridine. Phenol HILIC : μm, x. mm I.D Eluent : A)CH CN B) mm HCNH A/B = 9/, v/v Flow Rate :. ml /min Col. Temp. : Detection :UV nm Amide RP : μm, x. mm I.D Eluent : A) CH H B) H A/B = /7, v/v Flow Rate :. ml /min Col. Temp. : Detection :UV nm AQ-C Comparison between Amide vs C & AQ-C stain Amide T T Amide min AQ-C AQ-C T... : μm, x. Time mm (min) I.D : μm, x. mm I.D Eluent min :A) CH CN B) mmhcnh A/B = 9/, v/vt Eluent min : mmnah P :CH CN = 9:, v/v Flow Rate :. ml /min Flow Rate :. ml/min Col. Temp. : : μm, x. mm I.D Col. Temp. : x. mm I.D : μm, Eluent Detection A/B = 9/, v/v :A) :UV CH CNnm B) mmhcnh Eluent Detection : mmnah nm P :CH CN = 9:, v/v :UV Flow Rate. :. ml /min. Flow Rate :. ml/min. Col. Temp. : Col. Temp. : Detection :UV nm Detection :UV nm : μm, x. mm I.D Eluent : mmnah P :CH CN = 9:, v/v Flow Rate :. ml/min Col. Temp. : Detection :UV nm. mm I.D B) mmhcnh A/B = 9/, v/v min T. Cyanulic Acid. Melamine. Ammelide. Ammeline... min min. Cyanulic Acid. Melamine. Ammelide. Amm. Cyanulic Acid. Melamine. Ammelide. Ammel sales representative for ordering information or contact...77

8 Complete Selection verview C (m / g) Spherical. ctadecyl Group Complete % - Swift C.9 (m / g) Spherical. ctadecyl Group Complete 9% - AQ-C.9 (m / g) Spherical. ctadecyl Group Complete % - PhenylHexyl (m / g) Spherical. PhenylHexyl Group Complete 9% - Phenyl (m / g) Spherical. Phenyl Group No % -7. NH (m / g) Spherical. Aminopropyl Group No 7% -7. Amide μm (m / g) Spherical. Carbamoyl Group No % -. sales representative for ordering information or contact...77

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