Reversed Phase Columns. SEC Columns. Ion Exchange Columns. Application Specific Columns. Guard Columns. HILIC Columns. Normal Phase Columns.

Transcription

1

2 C o n t e n t s Column Selection Overview Ex Reversed-Phase Column Selection Guide Ex5 Selectivity Comparison of Reversed-Phase Ex7 Normal-Phase Column Selection Guide Ex0 Column Specifications...Ex Conforming to USP...Ex GL Sciences greatest strength...ex7 QC, ISO...Ex9 Reversed Phase InertSustain C8 00 InertSustainSwift TM C8 006 Inertsil ODS- 008 Inertsil ODS- 00 Inertsil ODS-V, ODS-V 0 Inertsil ODS-SP 0 Inertsil ODS-P 06 Inertsil ODS-EP 08 Inertsil ODS-80A 00 Inertsil ODS- 0 Inertsil ODS 0 InertSustain C8 0 Inertsil C8-06 Inertsil C8-08 Inertsil C8 00 Inertsil C 0 InertSustain Phenyl 0 InertSustain Phenylhexyl 0 Inertsil Ph- 06 Inertsil Ph 08 Inertsil CN- 00 Inertsil WP00 C8 0 Inertsil WP00 C8 0 Inertsil WP00 C 05 HILIC Inertsil Amide 08 Inertsil HILIC 050 InertSustain NH 05 Inertsil NH 05 SEC Inertsil Diol 07 Inertsil WP00 Diol 07 Ion Exchange Inertsil AX 078 Inertsil CX 080 Application Specific Bioptic AV-, AV- 08 Inertsil Peptides C8 085 Inertsil Acrolein C8 085 InertSphere TM Sugar- 086 Inertsil Sulfa C8 087 Inertsil AS 087 Corresponding Pharmacopeia (JP, USP, EP) 088 Corresponding SFC 090 Guard Guard Column s selection and Use 09 Cartridge Guard Column E 09 Cartridge Guard Column Ei (Non-metal type) 09 Guard for UHPLC 096 GL Cart 098 Packed Guard, Mini Guard 099 SILFILTER TM STD C8 0 Filters, Impurity Remove 05 Normal Phase Inertsil Diol 058 Inertsil SIL-00A 060 InertSustain NH 06 Inertsil NH 06 Inertsil CN- 066 Inertsil SIL-50A 068 Inertsil WP00 SIL 069 Ex

3 Reversed Phase 00 HILIC 07 Preparative Choosing Preparative 08 Preparative 09 Guard for Preparative JET 7 Other Preparative 7 Normal Phase 057 Capillary Capillary HPLC 0 Totally Porous Particle type Capillary HPLC Connection Kits for Totally Porous Particle type Capillary 6 Totally Porous Particle type Capillary Micro Guard 7 Monolithic Capillary HPLC (Available in South Asia, Middle East and Africa only) 8 MonoSpray TM SEC 07 Ion Exchange 077 Application Specific 08 Applications Applications 5 Guard 09 Index Cat. No. Index 6 Preparative 07 Capillary 9 Applications 5 Cat. No. Index 6 Ex

4 Column Selection Overview Separation Mode Selection for Analyte Polarity Water Soluble Analyte Properties Organic Solvent Soluble HILIC Ion pair + Reversed-Phase Reversed-Phase Normal-Phase Column Selection for Analyte Properties Vitamins Mixture of Water Soluble / Fat-Soluble Vitamins Reversed-Phase First choice For higher retention and separation efficiency InertSustain C8 (P.) Inertsil ODS-, ODS- (P.8, P.0) Vitamin C Derivatization Normal-Phase DNPH derivatized Vitamin C Inertsil SIL-00A (P.60) Direct Analysis HILIC Direct Vitamin C Analysis InertSustain NH (P.5) Inertsil Amide (P.8) Fat-Soluble Vitamins Vitamin D Vitamin E Normal-Phase Fat - Soluble Vitamin D and E InertSustain NH (P.6) Inertsil NH (P.6) Vitamin A Vitamin K Reversed-Phase Fat - Soluble Vitamin A and K InertSustain C8 (P.) Water Soluble vitamins Phase Detector Vitamin C NH, SIL, Amide UV Vitamin B ODS UV, (FL) Vitamin B ODS UV, FL Vitamin B6 ODS UV Niacin ODS UV Folic acid ODS UV Pantothenic acid ODS UV Fat-Soluble Vitamin Phase Detector Vitamin A ODS UV, FL Vitamin D ODS, NH UV Vitamin E ODS UV, FL Vitamin K ODS UV, (FL) (FL): Post-column derivatization Sugars Monosaccharides to oligosaccharides HILIC First choice For higher retention InertSustain NH (P.5) Inertsil NH (P.5) First choice InertSustain C8 (P.) Derivatized sugars Reversed-Phase For higher separation efficiency Inertsil ODS-, ODS- (P.8, P.0) Inertsil WP00 C8 (P.) Nucleic acid related molecules Nucleic-acide base Nuclesides Nucleotides Ion pair + Reversed-Phase First choice For higher separation efficiency InertSustain C8 (P.) Inertsil ODS-, ODS- (P.8, P.0) HILIC First choice For higher separation efficiency Inertsil Amide (P.8) Inertsil HILIC (P.50) First choice InertSustain C8 (P.) Reversed-Phase For big selectivity change For small selectivity change InertSustain Phenyl (P.) Inertsil ODS-, ODS- (P.8, P.0) Drugs HILIC For compounds that are weekly retained by ODS Inertsil Amide (P.8) Inertsil HILIC (P.50) Ex

5 Column Selection Overview Organic acids Common organic acids Reversed Phase First choice For higher retention and separation efficiency InertSustain C8 (P.) Inertsil ODS-, ODS- (P.8, P.0) Ion - exclusion Simultaneous analysis of organic acids Inertsil Ph- + Inertsil CX series (P.6 P.80) Oxalic acid HILIC Higher retentivity even for highly polar molecules Inertsil Amide (P.8) Amino acids Reversed Phase Analysis of Pre-column derivatized For higher retention and separation efficiency InertSustain C8 (P.) Inertsil ODS-, ODS- (P.8, P.0) Ion pair + Reversed-Phase Retain amino acids with ion pair reagent InertSustain C8 (P.) HILIC Higher retentivity even for highly polar compound Inertsil Amide (P.8) Ion-exchange Post-column HPLC on a polymer column Proteins Peptides Peptides Reversed Phase First choice For higher retention and separation efficiency Simultaneous analysis of organic acids InertSustain C8 (P.) Inertsil Peptides C8 (P.85) MonoCap C8 High Resolution 000 (P.9) Proteins Reversed Phase First choice For higher retentivity For weaker retentivity Inertsil WP00 C8 (P.) Inertsil WP00 C8 (P.) Inertsil WP00 C (P.5) Size exclusion For SEC analysis Inertsil WP00 Diol (P.7) High polar molecules HILIC For compounds that are weekly retained by ODS Inertsil Amide (P.8) Pesticides Veterinary medicines Reversed Phase First choice For big selectivity change For small selectivity change Sulfa analysis InertSustain C8 (P.) InertSustain Phenyl (P.) Inertsil ODS-, ODS- (P.8, P.0) Inertsil Sulfa C8 (P.87) Aldehydes Reversed Phase DNPH derivatized aldehydes DNPH derivatized acrolein InertSustain C8 (P.) Inertsil Acrolein C8 (P.85) Polymers Size exclusion Both water and organic solvents can be used. Inertsil WP00 Diol (P.7) Arsenic Organic and non organic arsenics Inertsil AS (P.87) Ex

6 Reversed-Phase Column Selection Guide Small Molecule Analysis MW<5000 First choice InertSustain C8 Hydrophobicity : Week Hydrophobicity : Moderate Hydrophobicity : Strong The change of retention For Ultra fast analysis Wide ph compatibillity Shorter retention than C8 InertSustainSwift TM C8 InertSustain C8 Shorter retention for hydrophobic compounds Inertsil ODS-SP Shorter retention than C8 Inertsil C First choice InertSustain C8 Non-planar molecule Planar molecule Choose from ODS columns Slightly higher steric selectivity Inertsil ODS- Higher steric selectivity Inertsil ODS-P The Change in Selectivity InertSustain C8 Hydrogen-bonding, π-π interactions : Week Hydrogen-bonding : Strong π-π interactions : Strong Provides big changes in the elution pattern by π-π interactions InertSustain Phenyl Choose from other than ODS columns Provides changes in the elution pattern by π-π interactions Inertsil Ph- Provides changes in the elution pattern by π-π interactions and hydrophobic interaction Inertsil Ph Provides changes in the elution pattern by hydrogen-bonding interaction Inertsil ODS-EP (Polar embeded group) Ex 5

7 Reversed-Phase Column Selection Guide Large Molecule Analysis MW>5000 First choice Inertsil WP00 C8 Best choice for large molecule analysis like protein analysis Hydrophobicity : Week Hydrophobicity : Moderate Hydrophobicity : Strong The change of retention Increasing retentivity in whole Inertsil WP00 C8 Faster separation Inertsil WP00 C (Polar embeded group) Inprove peak shapes of hydrophobic proteins Change in separation pattern by hydrogenbonding interaction Inertsil WP00 C (Polar embeded group) Small Molecule-Highly Polar Compound Analysis ODS Column Acidic polar compound Neutrality polar compound Basic polar compound Increasing retentivity for acidic compoundes ODS column + anionic ion-pair reagent Use ion-pair Increasing retentivity for basic compounds ODS column + cationic ion-pair reagent ODS Column Acidic polar compound Neutrality polar compound Basic polar compound Increasing retentivity for acidic, neutral and basic compounds Inertsil Amide HILIC Increasing retentivity for neutral and basic compounds Inertsil HILIC Ex 6

8 Selectivity Comparison of Reversed-Phase Selectivity of reversed-phase columns are compared under the same conditions, packing material size and column size. Chemical structural formulas used for the comparison are shown at right. Separation differences between basic compounds, acidic compounds, alkyl benzenes and polyaromatic compounds shows the differences of column selectivity. The more silanol groups on the packing material lead the later elution time of Caffeine compared with that of Phenol. The higher hydrophobicity of the column shows the later elution time of n-amylbenzene compared with that of n-butylbenzene. The higher steric selectivity of the column shows the later elution time of Triphenylene compared with that of o-terphenyl Column Eluent Flow Rate Col. Temp. Detection Injection Vol. : 5 μl* : Peversed-Phase columns (5 μm, 50.6 mm I.D.)* : A) CHOH B) HO : A/B = 80/0, v/v, (Inertsil Diol, CN-) A/B = 70/0, v/v, :.0 ml/min* : 0 C : UV 5 nm * : For Mono Clad C8-HS (.0 mm I.D.), 0. ml/min of flow rate and μl of injection volume are adopted according to the column internal diameter. O O N N OH HN O N N O N H. Uracil. Caffeine. Phenol. n-butylbenzene 6. n-amylbenzene 5. o-terphenyl 7. Triphenylene InertSustain C8 Recommended ODS column as the first choice. InertSustainSwift TM C8 7 For first elution with a sharp peak shape. Suitable for MS analysis 7 5, Inertsil ODS- Slightly faster elution time than InertSustain C8 and slightly higher planar molecule recognition (steric selectivity). 7 Inertsil ODS- Shows similar elution pattern with InertSustain C8 7 5, Inertsil ODS- Adequate retention and steric selectivity Inertsil ODS Original version of Inertsil column Inertsil ODS-EP Shows a different separation pattern from other ODS columns Suitable for a ODS column being specified still in need of a separation changes Inertsil ODS-P 5 6 High steric selectivity shows highest resolution between peek 5 and Ex 7

9 Selectivity Comparison of Reversed-Phase Inertsil ODS-SP With common retentivity for hydrophilic compounds (Peal,,,) and much less retentivity for hydrophobic compounds (Peak,5,6,7) 7 Inertsil WP00 C8 7 Weakly retain both hydrophilic compounds and hydrophobic compounds by smaller surface area Inertsil ODS-80A Shows high plate count owing to narrow pore size Mono Clad C8-HS Weakly retain compounds compared with packed columns InertSustain C8 Recommended C8 column as the first choice Inertsil C8- Shows first elution of compounds and slightly higher steric selectivity , Inertsil C8- Shows similar retentivity to InertSustain C8 but slightly different selectivity. Inertsil WP00 C8 Weakly retain both hydrophilic compounds and hydrophobic compounds by smaller surface area. 7 5,6 7 5, Inertsil C To reduce retention time for hydrophobic compounds. Inertsil WP00 C Suitable for hydrophobic protein analysis , Ex 8

10 Selectivity Comparison of Reversed-Phase InertSustain Phenyl Provide with big changes in elution order of C8 and C and recommended for such perposes Inertsil Ph- Compared to InertSustain Phenyl, different separation pattern can be obtained owning to steric selectivity difference. 7 5, Inertsil Ph As the bonded phase includes an alkyl chain, the retentivity for hydrophobic compounds are higher. 5,7 Inertsil CN- Relatively strong π-π interactions and hydrogen-bonding capacity resulting in higher retention time of aromatic compounds are shown Inertsil Diol,,6 Strong hydrogen-bonding interaction with diol groups and very weak hydrophobic interaction found from co-elution of peak and 6 are observed The strengths of stereo selectivity and π-π interaction against retention strengths of reversed phase columns are shown below. The following contents are pointed in the chart. Retentive factor of amil benzene as retentivity. Relative retention of o-terphenyl and triphenylene as stereo selectivity. Relative retention value of amilbenzene and triphenylene as strength of π-π interaction. type was plotted to discriminate from the other columns since polar group endohedral (embedded type) columns InertSustain & Inertsil Distribution Model Stereoselectivity (o-terphenyl and triphenylene) Interaction by polar functional group are reflected since embed packing material. Inertsil ODS-EP Inertsil WP00 C Inertsil ODS-P.6 MonoClad C8-HS Inertsil ODS-.5 Inertsil WP00 C8 Inertsil WP00 C8 Inertsil ODS-. Inertsil ODS-80A Inertsil C8-. InertSustainSwift C8 Inertsil C. Inertsil ODS- Inertsil C8- Inertsil ODS. InertSustain C8 Inertsil ODS-SP InertSustain C Relative Retention (k of Amylbenzene) Strength of π electron interaction (amilbenzene and triphenylene) InertSustain Phenyl Inertsil Ph- Inertsil Ph (InertSustain C8) (InertSustain C8) Relative Retention (k of Amylbenzene) Interaction may changes upon eluent conditions. This chart shows typical guidance. Ex 9

11 Normal-Phase Column Selection Guide Low Molecular Samples on Normal Distribution Mode First choice Inertsil Diol Faster elution by decrease in surface area Inertsil WP00 Diol Decrease in retention Faster elution overall Inertsil WP00 SIL Retention and separation by amino polarity InertSustain NH Alternative selectivity Retention and separation by silanol only Inertsil SIL-00A Retention and separation by cyano polarization Inertsil CN- Size Exclusion Chromatography (SEC) Low Molecular Weight Indication of exclusion limit MW 0,000 Water soluble, Organic solvent soluble SEC Organic solvent soluble SEC Inertsil Diol Inertsil SIL-00A High Molecular Weight Indication of exclusion limit MW 00,000 Water soluble, Organic solvent soluble SEC Organic solvent soluble SEC Inertsil WP00 Diol Inertsil WP00 SIL Ex 0

12 Column Specifications ODS Reversed-Phases The other Reversed-Phases HILIC Normal- Phases Phase Features USP Code (μm) Pore Size (nm) InertSustain C8 First choice with ultra high inertness and high durability L,, InertSustainSwift C8 First analysis with ultra high inertness and high durability L.9,, Inertsil ODS- Ultra high inertness, High plate count,,medium retentivity L,, Inertsil ODS-V Inertsil ODS- Validated column L, Inertsil ODS- Strong retentivity, Lower column backpressure, Very inert L,,, 5, Inertsil ODS-V Inertsil ODS- Validated column L, Inertsil ODS-SP Weak retentivity, for hydrophobic compounds L, Inertsil ODS-P High steric selectivity L, Inertsil ODS-EP A polar functional group embedded L Inertsil WP00 C8 Analysis of high molecules L Inertsil ODS-80A Elute low molecule with a sharp peak shape L Inertsil ODS- Ultra pure silica gel is used L Inertsil ODS Inertness st generation L 5, InertSustain C8 First choice with ultra high inertness and high durability L7,, Inertsil C8- Ultra high inertness, High plate count,, low retentivity L7,, Inertsil C8- Strong retentivity, Lower column backpressure, Very inert L7,, 5, Inertsil C8 Ultra pure silica gel is used L Inertsil C Low retentivity L Inertsil WP00 C8 Suitable for high molecules L Inertsil WP00 C Suitable for high molecules L InertSustain Phenyl Extremely strong π-π interactions L,, Inertsil Ph- strong π-π interactions L,, Inertsil Ph High inertness, weak π-π interactions L Inertsil HILIC Separation of highly polar basic compounds L0, Inertsil Amide Separation of polar compounds, InertSustain NH Sugar analysis L8, Inertsil NH Sugar analysis, High retentive in normal-phase mode L8, Inertsil Diol First choice for normal-phase mode, For SEC L0, Inertsil SIL-00A Ultra pure silica gel with 00Å pore size L, Inertsil SIL-50A Ultra pure silica gel with 50Å pore size L Inertsil WP00 SIL Ultra pure silica gel with 00Å pore size L Inertsil CN- Can be also used in reversed-phase mode L0, SEC Inertsil WP00 Diol High molecule SEC, Can be also used in normal-phase mode L0, L Ion- Exchanges The others Inertsil AX Anion-exchange column Inertsil CX Cation - exchange column L Bioptic AV- Chiral separation in reversed-phase mode 5 0 Bioptic AV- Inner reversed-phase separation 5 0 Inertsil Peptides C8 Peptide analysis L 0 50 Inertsil Acrolein C8 Acrolein analysis L InertSphere Sugar- Sugar analysis with ECD 5 Inertsil Sulfa C8 Sulfa Analysis L, MonoCap Series Monolithic silica capillary column Area (m /g) Ex

13 Column Specifications ODS Reversed-Phases The other Reversed-Phases HILIC Normal- Phases phase Carbon loading (%) End Cap Inertness Recommended ph range Analytical columns Reference pages Preparative columns InertSustain C8 ~0 09 InertSustainSwift C8 9 ~0 6 * * Inertsil ODS- ~ Inertsil ODS-V ~7.5 * * Inertsil ODS- 5 ~ Inertsil ODS-V 5 ~7.5 * * Inertsil ODS-SP 8.5 ~ Inertsil ODS-P 9 ~ Inertsil ODS-EP 9 ~ Inertsil WP00 C8 9 ~7.5 Inertsil ODS-80A 7.5 ~ * Inertsil ODS- 8.5 ~7.5 0 * Inertsil ODS ~7.5 0 * InertSustain C8 8 ~0 0 Inertsil C8-5 ~ Inertsil C8-9 ~ Inertsil C8 0.5 ~7.5 0 * * Inertsil C 7.5 ~7.5 * * Inertsil WP00 C8 ~7.5 Inertsil WP00 C ~7.5 5 InertSustain Phenyl 0 ~7.5 Inertsil Ph- 9.5 ~7.5 6 Inertsil Ph 0 ~7.5 8 * Inertsil HILIC 0 ~ Inertsil Amide 8 ~7.5 8 InertSustain NH 7 ~7.5 5, 6 Inertsil NH 8 ~7.5 5, 6 Inertsil Diol 0 ~7.5 58, 7 5 Inertsil SIL-00A ~ Inertsil SIL-50A ~ * Inertsil WP00 SIL ~ * Inertsil CN- ~7.5 0, 66 5 SEC Inertsil WP00 Diol 9 ~7.5 7 * Ion- Exchanges The others Inertsil AX 7 ~ * 5 Inertsil CX ~ * 5 Bioptic AV- ~7.5 8 * Bioptic AV- ~7.5 8 * Inertsil Peptides C8 5 ~ * Inertsil Acrolein C8 9 ~ * InertSphere Sugar- ~ 86 Inertsil Sulfa C8 5 ~ * * MonoCap Series : Please refer to GL Sciences website. : Please contact us. Capillary columns Ex

14 Conforming to USP USP Code PACKING HPLC Column Name L L L Octadecyl silane chemically bonded to porous or nonporous silica or ceramic microparticles,.5 to 0 µm in diameter, or a monolithic silica rod. Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 0 to 50 µm in diameter. Porous silica particles,.5 to 0 µm in diameter, or a monolithic silica rod. InertSustain C8 InertSustainSwift C8 Inertsil ODS- Inertsil ODS- Inertsil ODS-SP Inertsil ODS-P Inertsil ODS- Inertsil ODS-80A Inertsil ODS Inertsil WP00 C8 Unisil Q C8, NQ C8 ECONO PREP ODS Inertsil SIL-00A Inertsil SIL-50A Inertsil WP00 SIL Unisil Q 0, Q 60, Q 00 L Silica gel of controlled surface porosity bonded to a solid spherical core, 0 to 50 µm in diameter. ECONO PREP SIL L5 Alumina of controlled surface porosity bonded to a solid spherical core, 0 to 50 µm in diameter. L6 Strong cation-exchange packing-sulfonated fluorocarbon polymer coated on a solid spherical core, 0 to 50 µm in diameter. L7 L8 L9 L0 L Octylsilane chemically bonded to totally porous or superficially porous silica particles,.5-0 µm in diameter, or a monolithic silica rod. An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support,.5 to 0 µm in diameter. Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, to0 µm in diameter. Nitrile groups chemically bonded to porous silica particles,.5 to 0 µm in diameter. Phenyl groups chemically bonded to porous silica particles,.5 to 0 µm in diameter. InertSustain C8 Inertsil C8- Inertsil C8- Inertsil C8 Inertsil WP00 C8 Unisil Q C8 InertSustain NH Inertsil NH Unisil Q NH Inertsil CX Inertsil CN- Unisil Q CN InertSustain Phenyl Inertsil Ph- Inertsil Ph L A strong anion-exchange packing made by chemically bonding a quartenary amine to a solid silica spherical core, 0 to 50 µm in diameter. L Trimethylsilane chemically bonded to porous silica particles, to 0 µm in diameter. Spherisorb Methyl L Silica gel having a chemicallly bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 0 µm in diameter. Nucleosil 00-SB Partisil SAX Spherisorb SAX L5 Hexylsilane chemically bonded to totally porous silica particles, to 0 µm in diameter. Spherisorb C6 L6 Dimethylsilane chemically bonded to porous silica particles, 5 to 0 µm in diameter. L7 L8 L9 L0 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to µm in diameter. Amino and cyano groups chemically bonded to porous silica particles, to 0 µm in diameter. Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, about 9 µm in diameter. Dihydroxypropane groups chemically bonded to porous silica particles,.5 to 0 µm in diameter. PRP-X00, PRP-X00 HC-75(H + ) SUGAR SH0, SH8 RSpak KC-8 IC Y-5 Partisil 5 PAC Partisil 0 PAC HC-75(Ca + ) SUGAR SC0, SC8 SUGAR SC Inertsil Diol Inertsil WP00 Diol PROTEIN KW-800 series Ex

15 Conforming to USP USP Code PACKING HPLC Column Name L L L L L5 L6 A rigid, spherical styrene-divinylbenzene copolymer, to 0 µm in diameter. A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, about 0 µm in size. An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, about 7 - µm in size. A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on the matrix surface, to 6 µm in diameter. Packing having the capacity to separate compounds with a molecular weight range from (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated ether (surface contained some residual carboxyl functional groups) was found suitable. Butyl silane chemically bonded to totally porous silica particles,.5 to 0 µm in diameter. PRP-, PRP- GPC KF-80 RSpak DS-, DS-6 RSpak RP8-5 PRP-X00, PRP-X00 SUGAR SH0, SH8 RSpak KC-8 SUGAR SP080 SUGAR SC0, SC8 SUGAR SZ55 SUGAR KS800 series IC Y-5 PRP-X500 IEC QA-85 OHpak SB-80 HQ OHpak SB-80.5 HQ Inertsil C Inertsil WP00 C L7 Porous silica particles, 0 to 50 µm in diameter. A multifunctional support, which consists of a high purity, 00 Å, spherical silica substrate that has been L8 bonded with anionic exchanger, amine functionality in addition to a conventional reversed phase C8 functionality. L9 Gamma alumina, reverse-phase, low carbon percentage by weight, alumina-based polybutadiene spherical particles, 5 µm in diameter with a pore volume of 80 Å. L0 Ethyl silane chemically bonded to totally porous silica particles, to 0 µm in diameter. A hydroxide-selective, strong anion-exchange resin-quaternary amine bonded on latex particles attached to L a core of 8.5 µm macroporous particles having a pore size of 000 Å and consisting of ethylvinylbenzene cross-linked with 55 % divinylbenzene. L A chiral ligand-exchange packing-l-proline copper complex covalently bonded to irregularly shaped silica CHIRALPAK WH particles, 5 to 0 µm in diameter. L Packing having the capacity to separate dextrans by molecular size over a range of,000 to 500,000 Da. It Inertsil WP00 Diol is spherical, silica-based, and processed to provide ph stability. PROTEIN KW-800 series L Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the HC-75(Pb + ) lead form, 7 to 9 µm in diameter. SUGAR SP080 L5 A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase having a pore size of 50 Å. L6 A, 5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5-µm aminopropyl silica. SUMICHIRAL OA-000 L7 Packing having the capacity to separate proteins by molecular size over a range of,000 to 0,000 Da. It is OHpak SB-80 HQ a polymethacrylate gel. L8 A methacrylate-based size-exclusion packing for water-soluble samples. OHpak SB-800 HQ series L9 A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin. OHpak SB-800 HQ series RSpak DM-6 L0 Cellulose tris-,5-dimethylphenylcarbamate coated porous silica particles, 5 to 0 µm in diameter. CHIRALCEL OD series L9 A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin. OHpak SB-800 HQ series RSpak DM-6 L0 Cellulose tris-,5-dimethylphenylcarbamate coated porous silica particles, 5 to 0 µm in diameter. CHIRALCEL OD series Ex

16 Conforming to USP USP Code PACKING HPLC Column Name L Immobilized α -acid glycoprotein on spherical silica particles, 5 µm in diameter. CHIRALPAK AGP L L L L5 L6 L7 L8 L9 L50 Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5 µm in diameter. Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer,.5 to 0 µm in diameter. A multifunctional support, which consists of a high purity, 60 Å, spherical silica substrate that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a conventional reversed phase C8 functionality. Beta cyclodextrin bonded to porous silica particles, 5 to 0 µm in diameter. Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads, about 9 to µm in diameter. High-capacity anion-exchange microporous substrate, fully functionalized with trimethylamine groups, 8 µm in diameter. Sulfonated, cross-linked polystyrene with an outer layer of submicron, porous, anion-exchange microbeads, 5 µm in diameter. A reversed-phase packing made by coating a thin layer of polybutadiene onto spherical porous zirconia particles, to 0 µm in diameter. Multifunction resin with reversed-phase retention and strong anion-exchange functionalities. The resin consists of ethylvinylbenzene, 55 % cross-linked with divinylbenzene copolymer, to 5 µm in diameter, and a surface area not less than 50 m² per g. Substrate is coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene. ORpak CDBS-5 SUMICHIRAL OA7000 SUMICHIRAL OA700 PRP-X00, PRP-X0 RCX-0, RCX-0 L5 Amylose tris-,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 5 to 0 µm in diameter. CHIRALPAK AD series L5 A strong cation-exchange resin made of porous silica with sulfopropyl groups, 5 to 0 µm in diameter. L5 Weak cation-exchange resin consisting of ethylvinylbenzene, 55 % cross-linked with divinylbenzene copolymer, to 5 µm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500 µeq/column. L5 A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, about µm in diameter. L55 A strong cation-exchange resin made of porous silica coated with polybutadiene-maleic acid copolymer, Spherisorb SCX about 5 µm in diameter. L56 Propyl silane chemically bonded to totally porous silica particles, to 0 µm in diameter. L57 A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 µm in diameter, with a pore size of 0 Å. L58 L59 L60 L6 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 0 µm diameter. Packing for the size-exclusion separation of proteins (separation by molecular weight) over the range of 5 to 7,000 kda. The packing is a spherical.5 - to 0-µm silica of hybrid packing with a hydrophilic coating. Spherical, porous silica gel, 0 µm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and endcapped. A hydroxide selective strong anion-exchange resin consisting of a highly cross-linked core of -µm microporous particles having a pore size less than 0 Å units and consisting of ethylvinylbenzene crosslinked with 55 % divinylbenzene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quaternary ammonium ions (6 %). SUGAR KS 800 series CXpak P-S PROTEIN KW-80 Ex 5

17 Conforming to USP USP Code PACKING HPLC Column Name L6 C0 silane bonded phase on a fully porous spherical silica, to 5 µm in diameter. L6 Glycopeptide teicoplanin linked through multiple covalent bonds to a 00-Å units spherical silica. SUGAR KS 800 series L6 Strongly basic anion-exchange resin consisting of 8 % cross-linked styrene-divinylbenzene copolymer with a quaternary ammonium group in the chloride form, 5 to 80 µm in diameter. L65 Strongly acidic cation-exchange resin consisting of 8 % sulfonated cross-linked styrene-divinylbenzene copolymer with a sulfonic acid group in the hydrogen form, 5 to 50 µm in diameter. L66 A crown ether coated on a 5-µm particle size silica gel substrate. The active site is (S)-8-crown-6-ether. Crownpak CR (+) L67 L68 L69 L70 L7 Porous vinyl alcohol copolymer with a C8 alkyl group attached to the hydroxyl group of the polymer, to 0 µm in diameter. Spherical, porous silica, 0 µm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not endcapped. Ethylvinylbenzene/divinylbenzene substrate agglomerated with quaternary amine functionalized 0-nm latex beads, about 6.5 µm in diameter. Cellulose tris(phenyl carbamate) coated on 5-µm silica. A rigid, spherical polymetacrylate, to 6 µm in diameter. Asahipak ODP-0 Asahipak ODP-50 Shodex ET-RP CHIRALCEL OC-H SUMICHIRAL OA-00 RSpak DE-, DE- RSpak DE-6 L7 (S)-phenylglycine and,5-dinitroanaline urea linkage covalently bonded to silica. SUMICHIRAL OA-00 L7 A rigid spherical polydivinylbenzene particle, 5 to 0 µm in diameter. L7 a strong anion-exchange resin consisting of a highly cross-linked core of 7- µm macroporous particles having a 00-Å average pore size and consisting of ethylvinylbenzene cross-linked with 55 % divinylbenzene and an anion-exchange layer grafted to the surface, which is functionalized with alkyl quaternary ammonium ions. L75 A chiral-recognition protein, bovine serum albumine (BSA), chemically bonded to silica particles, about 7 µm in diameter, with a pore size of 00 Å. L76 Silica based weak cation-exchange material, 5 µm in diameter. Substrate is surface polymerized polybutadiene-maleic acid to provide carboxylic acid functionalities. Capacity not less than 9 µeq/column. L77 Weak cation-exchange resin consisting of ethylvinylbenzene, 55 % cross-linked with divinylbenzene copolymer, 6 to 9 µm diameter. Substrate is surface grafted with carboxylic acid funcionalized groups. Capacity not less than 500 µeq/column ( mm 5 cm) L78 A silane ligand that consists of both reversed-phase (an alkyl chain longer than C8) and anion-exchange (primary, secondary, tertiary, or quartenary amino groups) functional groups chemically bonded to porous or non-porous silica or ceramic micro-particles,.0 to 50 µm in diameter, or a monolithic rod. L79 A chiral-recognition protein, human serum albumin (HSA), chemically bonded to silica particles, about 5 µm CHIRALPAK HSA in diameter. L80 Cellulose tris(-methylbenzoate)-coated, porous, spherical, silica particles, 5 µm in diameter. CHIRALCEL OJ CHIRALCEL OJ-H A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 9 µm porous L8 particles having a pore size of 000 Angstroms units and consisting of ethylvinylbenzene cross-linked with 55 % divinylbenzene with a latex coating composed of 70 nm diameter microbeads (6 % crosslinked) bonded with alkanol quartenary ammonium ions. Ex 6

18 GL Sciences greatest strength GL Sciences supports from the use of SPE to analysis. Among them all, HPLC columns which play a major role in separation analysis are manufactured by synthesizing base silica-gels, bonging phases and being packed into columns and through demanding tests for a stable supply with the same excellent quality all over the world. Based on accumulated know-how GL Sciences manufacturing technology keeps on evolving to supply better columns for customers Sophisticated self-manufacturing technology for the base silica GL Sciences has been synthesizing base silica-gels, bonding phases and endcapping for column packing which enable us stable supply with exceptionally-high quality. Consequently those HPLC columns have been chosen for the method used for many years as reliable products. Ultra pure base silica Reliable column performance Provide the same separation patterns with the changes of particle size Test by a strong basic compound Instead of a difficult compound symmetry peak and good reproducibility are obtained 5 μm 0.0 MPa μm.8 MPa μm. MPa Column : InertSustain C8 (50. mm I.D.) Eluent : A) CHOH B) 5 mm phosphate buffer (ph 7.0) A/B = 0/70, v/v Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : UV 0 nm Sample :. Uracil. Pyridine. Phenol. Berberine chloride 5. Dextromethorphan Lot. Lot. Lot Column : InertSustain C8 (5 μm, 50.6 mm I.D.) Eluent : A) CHCN B) 5 mm phosphate buffer (ph 7.0) A/B = 0/60, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 0 nm Sample :. Dextromethorphan Base silica is designed for low column back pressure to reduce the load on the system Column : 50.6 mm I.D. Flow Rate : ml/min Col.Temp. : 0 C Inertsil ODS- Company A Company B Company C Company D Company E Column : 50.6 mm I.D. Flow Rate : ml/min Col.Temp. : 0 C Inertsil ODS- Company B Company D Company A Company C Company E Pressure(MPa) Pressure(MPa) CHCN Concentration (%) CHOH Concentration (%) Ex 7

19 GL Sciences greatest strength The Evolving HPLC Column Packings GL Sciences has been steadily supplying with columns from Inertsil ODS, first-generation to InertSustain series, integration of state-ofthe-art technologies, and has established a very good reputation around the world. They are available with the same quality and the same performance anywhere in the world. Europe Asia North America Africa Oceania South America Inertsil Series Inertsil Series 00 Å 50 Å 50 m /g 0 m /g Inertsil ODS Inertsil ODS- Inertsil Ph etc. 80 Å 50 m /g Inertsil ODS-80A Inertsil Series InertSustain Series 00 Å 00 Å 50 m /g 50 m /g For Low Molecular For High Molecular 00 Å Inertsil WP Series : Inertsil WP00 C8 Inertsil WP00 C8 Inertsil WP00 C Inertsil WP00 DIOL Inertsil WP00 SIL Inertsil ODS- Inertsil ODS- Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP Inertsil C8- Inertsil Ph- Inertsil CN- Inertsil HILIC Inertsil Amide Inertsil SIL-00A etc. 00 Å 50 m /g InertSustain InertSustain InertSustain InertSustain 00 m /g C8 C8 Phenyl NH InertSustainSwift C8 Base on Evolutionally Surfaced Silica (ES Silica) evolved from Inertsil, InertSustain has developed InertSustain employs a radically new type of silica, in which the surface of the silica is uniquely modified, the amount of silanols are controlled. Owing to this state-of-the-art technology ES silica allows easy surface modification including endcapping, which grants the following three favors.. Exceptionally improved inertness. Robust bonded phase. High reproducibility batch to batch Owing to above benefits InertSustain is recommended as the first choice column for almost all compounds analyzed. Batch to batch reproducibility Keeping on stable supplies with high quality and performance, GL Sciences continues to evolve. Year 0 TQ5-68 Year 0 TQ5-95 Year 0 TQ5-6 Year 00 TQ5-08 Year 009 TQ5-86 Year 008 TQ5-688 Year 007 TQ5-556 Year 006 TQ5-8 Year 005 TQ5-9 Year 00 TQ5-0 Year 00 TQ5-957 Year 00 TQ5-86 Year 00 TQ5-688 Year 000 TQ5-5 Year 999 TQ5-56 Column Eluent Flow Rate Col. Temp. Detection : Inertsil ODS- (5 μm, 50.6 mm I.D.) : CHOH/HO=80/0,v/v :.0 ml/min : 0 C : UV 5 nm Sample : ) Uracil ) Caffeine ) Phenol ) Butylbenzene 5) o-terphenyl 6) Amylbenzene 7) Triphenylene Ex 8

20 QC, ISO Quality Inspections Sphericity and surface smoothness of Silica-gel with Scanning Electron Microscopy. Particle size, Surface area, Pore diameter, Pore volume of Base Silica-Gel. Trace metals impurity on Base Silica-Gel Chemical bonding amount Residual Silanol Groups by 9Si-NMR Chromatographic Test for each lot ( Standard Samples) Column Performance Tests for Individual ISO Certification GL Sciences is ISO00 Compliant Company Product Ranges: Development, manufacture and sale of instruments, parts, accessories, columns, packing materials, reagents relating to gas chromatography, liquid chromatography and cells for spectrometry GL Sciences Fukushima Factory is ISO900 Compliant Facility Product Ranges: Design, manufacture and supply of instruments, parts, accessories, columns, packings, reagents relating to gas chromatography, liquid chromatography and cells for spectrometry General Technical Division Fukushima Factory Ex 9

21 Reversed Phase ---- InertSustain C8 00 InertSustainSwift TM C8 006 Inertsil ODS- 008 Inertsil ODS- 00 Inertsil ODS-V, ODS-V 0 Inertsil ODS-SP 0 Inertsil ODS-P 06 Inertsil ODS-EP 08 Inertsil ODS-80A 00 Inertsil ODS- 0 Inertsil ODS 0 InertSustain C8 0 Inertsil C8-06 Inertsil C8-08 Inertsil C8 00 Inertsil C 0 InertSustain Phenyl 0 InertSustain Phenylhexyl 0 Inertsil Ph- 06 Inertsil Ph 08 Inertsil CN- 00 Inertsil WP00 C8 0 Inertsil WP00 C8 0 Inertsil WP00 C 05 00

22 InertSustain C8 Physical Properties Silica : Newly Developed ES Silica Gel : μm, μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume : 0.85 ml/g Bonded Phase : Octadecyl Groups End-capping : Complete Carbon Loading : % USP Code : L ph Range : ~0 Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain C8 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties. InertSustain C8 inherits the advantages of all the current Inertsil HPLC columns (e.g., extremely low operating back pressure, superior inertness to typically any analytes, high efficiency and compatibility with a wide range of solvents), but now can be used for wide ph analysis with consistent performance from column to column and lot to lot. Figure : Basic Compounds InertSustain C8 X SELECT CSH C8 ZORBAX Eclipse Plus C8 Figure : Acidic Compounds No peak InertSustain C8 X SELECT CSH C8 ZORBAX Eclipse Plus C Column Size Eluent Flow Rate Col. Temp. Detection Sample Column Size Eluent Flow Rate Col. Temp. Detection Sample : μm, 50. mm I.D. : A) CHCN B) 5 mm KHPO (ph 7.0, KHPO) A/B = 0/70, v/v : 0. ml/min : 0 C : UV 0 nm :. Uracil. Pyridine. Phenol. Berberine chloride 5. Dextromethorphan : μm, 50. mm I.D. : A) CHCN B) 0. % HPO A/B = 5/75, v/v : 0. ml/min : 0 C : UV 5 nm :. Brilliant Blue FCF. Phenol. Salicylic acid Figure : Chelating Compound InertSustain C8 st Injection X SELECT CSH st Injection : No Peak Column Size Eluent Flow Rate Col. Temp. Detection Sample : 5 μm, 50.6 mm I. D. : A) CHCN B) 0. % HPO A / B = 0 / 60, v / v :.0 ml / min : 0 C : UV 5 nm :. Hinokitiol Time (min) Time (min) nd Injection nd Injection Time (min) Time (min) 00

23 InertSustain C8 Comparison of Performance to Core-Shell As shown below, core-shell columns show peak tailing due to the presence of trace metals or silanol groups in their silica gel. Quantitative and qualitative analysis will be a source of concern since the adsorption of compounds can be expected. InertSustain C8 µm Kinetex C8 (.7 μm) Intensity, cps.5e.0e 5.0e Intensity, cps.5e.0e 5.0e 0.0 Adsorption of Peak Column Eluent Flow Rate Col. Temp. Detection Injection Vol. Sample : ODS Column(00. mm I.D.) : A) mm CHCOONH in 95 % CHCN B) mm CHCOONH in 0 % CHCN A / B = 0 / 80 - min 00 / min 00 / min - 0 / 80, v / v (Mixed by a gradient mixer) : 0. ml / min : 0 C : LC / MS / MS (000 QTRAP : ESI, Positive, MRM) : 0 μl :. Nitrofurazone (00 μg / L). Lasalocid A (00 μg / L) Wide ph compatibility with Long Column Lifetime As shown in the experiment below, due to the introduction of Evolved Surface Silica, InertSustain C8 maintained high efficiency and peak shape for 00 hours while other wide ph column brands failed InertSustain C8 Xterra MS C8 Gemini-NX Reversed Phase HILIC Normal Phase SEC % Initial N Hours of Purging(h) Purging Column Size : 5 μm, 50.6 mm I.D. Eluent Flow Rate Col. Temp. : A)CHOH B)50 mm Triethylamine (ph 0.0) A/B = 0/70, v/v :.0 ml/min : 50 C Analytical Eluent : A) CHCN B) HO A/B = 65/5, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Sample : Naphthalene Experience the InertSustain! (Inertness and Sustainability) Highly end-capped ODS column such as InertSustain C8 offers an opportunity to flush out contaminants from the column surface easily using an organic solvent. Coffee melanoidins are brown heterogeneous polymers contained in coffee. Its components are not clarified yet, but it is considered to contain several ionic compounds, which a poorly end-capped column will adsorb those ionic compounds leading to short column lifetime. As for ODS column, which is commonly used for HPLC and LC/MS/MS, its inertness has an influence not only on peak shape but also detection sensitivity and durability. It is highly recommended to use highly end-capped column which provides good peak shape for both basic and acidic compounds such as InertSustain C8. The packing material was visually confirmed by removing the column Flow Before Experiment Injection of Coffee After loading Coffee Brand A After washing the column with CH CN 00 % 0 minute washing Ionic contaminants were hard to be washed out from the Column Still Contaminated InertSustain C8 Clean! Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 00

24 InertSustain C8 Reliable Reproducibility, Performance and Quality Rigorous quality control of physical properties and strict chromatographic tests for inertness and selectivity, contribute to the production of InertSustain C8 with an outstanding reproducibility and long column lifetime. Strong Basic Compound Test Superior peak shape with highly reproducible chromatograms can be obtained even for those strong basic compounds. Lot. Same Retention Behavior between Various s Reliable reproducibility and same retention behavior between various particle size columns 5 µm 0.0 MPa µm.8 MPa Column Size Eluent Flow Rate Col. Temp. Detection Sample Lot. Lot. : 5 µm, 50.6 mm I.D. : A) CHCN B) 5 mm phosphate buffer (ph 7.0) A / B = 0 / 60, v / v :.0 ml / min : 0 C : UV 0 nm :. Dextromethorphan Column Size Eluent Flow Rate Col. Temp. Detection µm. MPa : 50. mm I.D. : A) CHOH B) 5 mm phosphate buffer (ph 7.0) A / B = 0 / 70, v / v : 0. ml / min : 0 C : UV 0 nm Sample :. Uracil. Pyridine. Phenol. Berberine chloride 5. Dextromethorphan Strong Acidic Compound Test Strong Chelating Compound Test Lot. Lot. Lot. Lot. Lot. Lot Column Size Eluent Flow Rate Col. Temp. Detection Sample : 5 µm, 50.6 mm I.D. : A) CHCN B) 0. % HPO A / B = 5 / 75, v / v :.0 ml / min : 0 C : UV 5 nm :. Brilliant Blue FCF. Phenol. Salicylic acid Column Size Eluent Flow Rate Col. Temp. Detection Sample : 5 µm, 50.6 mm I.D. : A) CHCN B)0. % HPO A / B = 0 / 60, v / v :.0 ml / min : 0 C : UV 5 nm :. Hinokitiol 00

25 InertSustain C8 Analytical : μm Length \ I.D Length \ I.D HPSeries : μm MPa (500 Bar) End-fittings are /6 Waters-compatible. UHPLC compatible end-fittings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6. Length \ I.D : μm Length \ I.D Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 005

26 InertSustainSwift TM C8 Physical Properties Silica : Newly Developed ES Silica Gel :.9 μm, μm, 5 μm Surface Area : 00 m /g Pore Size : 00 Å (0 nm) Pore Volume :.00 ml/g Bonded Phase : Octadecyl Groups End-capping : Complete Carbon Loading : 9.0 % USP Code : L ph Range :.0~0.0 As shown in figure, InertSustainSwift C8 maintains the same extreme inertness, wide ph range and provide rapid separations with symmetric peaks. The optimization of surface area, pore size and chemical bonding delivers superior peak shapes (Figure ). Figure proves InertSustainSwift C8 is also ideal for LC/MS/MS applications which offer highly sensitive results and enables MS compatible buffers to be used due to the extremely inert silica gel. Figure : Comparison of Retentivity. min InertSustainSwift C8 (.9 μm) 5 5. min InertSustain C8 ( μm) 6 Column Size Eluent Flow Rate Col. Temp. Detection Sample : 50. mm I.D. : A) CHOH B ) HO A/B = 80/0, v/v : 0. ml/min : 0 C : UV 5 nm :. Uracil. Toluene. Ethylbenzene. Propylbenzene 5. n-butylbenzene 6. n-amylbenzene Figure : Comparison of Efficiency 7. MPa N : 6500 N : MPa N : 00 N : 900 Column Size : 50. mm I.D. Eluent : A) CHOH B) HO A/B = 0/70, v/v Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : UV 5 nm Sample :. Pyridine. Phenol.0.0 InertSustainSwift C8 (.9 μm) Figure : Rapid LC/MS/MS Analysis of Basic Drugs Complete Separation in 60 Seconds Acguity BEH C8 (.7 μm) Column Eluent : InertSustainSwift C8(.9 μm, 50. mm I.D.) : A) 0 mm Ammonium Formate in HO B) 0 mm Ammonium Formate in CHOH A/B = 70/0 0. min - 0/ min - 0/00 0. min 0/ min -70/0-0.5 min - 70/0,v/v Flow Rate : 0.6 ml/min Col. Temp. : 0 C Detection : LC/MS/MS (ESI, Positive, MRM) Sample :. Acebutolol. Atenolol. Labetalol. Nadolol 5. Pindolol

27 InertSustainSwift C8 Analytical :.9 μm Length / I.D Length / I.D HP Series : μm Max. Operating Pressure : 50 MPa (500 Bar) End-fittings are /6 Parker style. For maximum operating pressure information, please refer to page 6. Length / I.D : μm Length / I.D Length / I.D : 5 μm Length / I.D End-fittings are /6 Parker style. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 007

28 Inertsil ODS- Physical Properties Silica : Series High Purity Silica Gel : μm, μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : % USP Code : L ph Range : ~ 7.5 Inertsil ODS- delivers the same extreme inertness to any type of compounds just like InertSustain C8 along with unprecedented stability under 00 % aqueous mobile phases for qualitative and quantitative analysis. However, as the base silica gel and carbon loading are different on Inertsil ODS-, differences in selectivity can be observed for certain analytes. Figure : Comparison of Selectivity between various GL Sciences High. InertSustain C8 Inertsil ODS- Inertsil ODS- Inertsil ODS-. InertSustain C8 Inertsil C8-. Inertsil C8- InertSustain Phenyl. Inertsil Ph- Stereoselectivity(Tri / o-ter). o-terphenyl.triphenylene Difference in Stereoselectivity Low Retentivity K (n-amylbenzen) Column Size : 5 μm, 50.6 mm I.D. Eluent : A) CHOH B) HO A/B = 70/0, v/v Flow Rate : 0.8 ml/min Col. Temp. : 0 C Detection : UV 5 nm Sample :. o-terphenyl. n-amylbenzene. Triphenylene Figure : Dewetting Test Reduction rate of retention time 9.8 % Reduction rate of retention time 6.0 % Reduction rate of retention time 8.0 % Column Size : ODS Column(5 μm, 50.6 mm I.D.) Eluent : HO Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Sample :. Cytosine. Uracil. Guanine. Thymine 5. Adenine Testing Procedure: ) 00 % water is introduced into column over 0 minutes. ) Conduct Analysis (Upper chromatogram) ) Stop flow for 5 minutes. ) 00 % water is introduced again into column over 0 minutes. 5) Stop flow for 5 minutes again. 6) 00 % water is introduced again into column over 5 minutes. 7) Conduct Analysis (Lower chromatogram) Inertsil ODS XBridge C8 Luna C8() 008

29 Inertsil ODS- Analytical : μm Length \ I.D Length \ I.D HPSeries : μm MPa (500 Bar) End-fittings are /6 Waters-compatible. UHPLC compatible end-fittings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6. Length \ I.D : μm Length \ I.D Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 009

30 Inertsil ODS- Physical Properties Silica : Series High Purity Silica Gel : μm, μm, μm, 5 μm, 7 μm, 0 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : 5 % USP Code : L ph Range : ~ 7.5 Inertsil ODS- is still GL Sciences most popular phase and continues to be used widely and reliably for long established methods in pharmaceutical, and contract research labs. As shown in figure, Inertsil ODS- has a relatively strong retentivity compared to commercially available ODS columns. In addition, the introduction of higher surface area silica provide high preparative loading capacity without sacrificing peak shape which is illustrated in figure. Figure : Comparison of Retentivity Inertsil ODS- 0 0 InertSustain C , Column Size : 5 μm, 50.6 mm I.D. Eluent : A) CHOH B) HO A/B= 80/0, v/v Flow Rate :.0 ml/min Col.Temp. : 0 C Detection : UV 5 nm Sample :. Uracil. Caffeine. Phenol. Butylbenzene 5. o -Terphenyl 6. Amylbenzene 7. Triphenylene 0 0 Inertsil ODS Figure : Comparison of Loading Capacity Peak Width Ratio* Commercially Available ODS Column B Commercially Available ODS Column A Inertsil ODS Loading Capacity (mg) Column Size : 5 μm, 50.6 mm I.D. Eluent : A) CHOH B) HO A/B = 90/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 70 nm Sample : tert-buthylbenzene (00 mg/ml) The loading capacity varies depending on the column I.D. size and length. Analytical : μm 00 Length \ I.D Length \ I.D HPSeries : μm MPa (500 Bar) End-fittings are /6 Waters-compatible. UHPLC compatible end-fittings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6.

31 Inertsil ODS- Analytical : μm : μm : 5 μm Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length \ I.D..6 : 0 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm μm 5 μm μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 0

32 Inertsil ODS-V (Specifically Qualified HPLC columns for GLP/GMP Compliance Validation) Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : % USP Code : L ph Range : ~ 7.5 Inertsil ODS- columns have proven superior worldwide for analysis of strong pharmaceutical bases, acids, chelating compounds, and zwitterions. The long-awaited validated Inertsil ODS-V has now been added to our product lineup. Each Inertsil ODS-V is delivered with a Manufacturer s Validation Certificate showing the detailed results of every QA and QC step in manufacturing. By choosing Inertsil ODS-V, you can be assured that you are using one of the most trusted and enduring HPLC columns for validation. Details of Column Performance Report (Front Page) Analytical Length \ I.D : μm Length \ I.D : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. 0

33 Inertsil ODS-V (Specifically Qualified HPLC columns for GLP/GMP Compliance Validation) Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume Bonded Phase End-capping :.05 ml/g : Octadecyl Groups : Yes Carbon Loading : 5 % USP Code : L ph Range : ~ 7.5 Reversed Phase HILIC Inertsil ODS-V offers all of the outstanding chromatographic benefits of Inertsil ODS- with the added benefit of a more thoroughly documented, validated QC procedure consistent with the demands of GLP/GMP compliance. Each Inertsil ODS-V is delivered with a Manufacturer s Validation Certificate showing the detailed results of every QA and QC step in manufacturing. The use of Inertsil ODS- V columns provides an extra measure of assurance of consistent performance from column to column and batch to batch. Inertsil ODS- V columns are also available in -column-sets packed with your choice of different silica batches or a single silica batch to assist in reproducibility studies. Details of Manufacturer's Validation Certificate (Back Page) Normal Phase SEC Ion Exchange Application Specific Analytical Length \ I.D : μm Length \ I.D : 5 μm Validation Packs (-Column-Sets) Inertsil ODS-V columns are also available in -column-sets packed with your choice of different silica batches or a single silica batch to assist in reproducibility studies. Choose the column dimension and one of the following batch requirements.all three columns with single batch. Two columns with single batch and other a different batch. All three columns with different batches Length \ I.D : 5 μm *End-fittings are /6 Waters-compatible. *For maximum operating pressure information, please refer to page 6. Guard Preparative Capillary Applications Cat. No. Index 0

34 Inertsil ODS-SP Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : 8.5 % USP Code : L ph Range : ~ 7.5 As shown in figure, Inertsil ODS-SP is super base deactivated and optimally bonded to retain hydrophilic compounds without excessive retention of hydrophobic compounds achieving better separations faster than before. As the carbon load of Inertsil ODS-SP is relatively low, it is compatible with 00 % aqueous eluents and offer faster equilibration of column for gradient analysis. Figure : Comparison of Retention Behavior between Inertsil ODS- and Inertsil ODS-SP Column Size : 5 μm, 50.6 mm I.D. Eluent Flow Rate Col. Temp. Detection : A) CHOH B) 0 mm NaHPO A/B = 0/90 0 min 50/50, v/v :.0 ml/min : 0 C : UV 80 nm Sample :. Gallocatechin (GC). Epigallocatechin (EGC). Catechin (C). Epigallocatechin gallate (EGCg) 5. Epicatechin (EC) 6. Gallocatechin gallate (GCg) 7. Epicatechin gallate (ECg) 8. Catechin gallate (Cg) : 5 μm, 50.6 mm I.D. Column Size Eluent Flow Rate Col. Temp. Detection : A) CHCN B) 0 mm KHPO (ph.0, HPO) A/B = 70/0, v/v :.0 ml/min : 0 C : UV 80 nm Sample :. Phenol. Bisphenol A. -Octylphenol. -Nonylphenol 7 8 Maintaining retention of Hydrophilic compounds 6 Faster elution of Hydrophobic compounds Inertsil ODS-SP Inertsil ODS-SP Inertsil ODS Inertsil ODS- Figure : Simultaneous Analysis of Soybean Isoflavone Column : Inertsil ODS-SP (5 μm, 50.6 mm I.D.) Eluent : A) 0. % CHCOOH in CHCN B) 0. % CHCOOH in HO A/B = 5/85 8 min 5/85 min 5/65 0 min hold, v/v Flow Rate :.5 ml/min Col. Temp. : 5 C Detection Sample : UV 5 nm :. Daidzin (D). Glycitin (Gly). Genistin (GI). 6 -O-malonyldaidzin (MD) O-malonylglycitin (MGI) O-acetyldaidzin (AD) O-acetylglycitin (AGI) O-malonylgenistin (MG) 9. Daizein (De) 0. Glycitein (Gle). 6 -O-acetylgenistin (AG). Genistein (Ge) 0

35 Inertsil ODS-SP Analytical Length \ I.D HPSeries : μm MPa (500 Bar) End-fittings are /6 Waters-compatible. UHPLC compatible end-fittings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6. Length \ I.D : μm Length \ I.D : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 05

36 Inertsil ODS-P Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octadecyl Groups End-capping : None Carbon Loading : 9 % USP Code : L ph Range : ~ 7.5 GL Sciences offers a polymerically bonded ODS-P phase which provide high steric selectivity for separation of planar and non-planar compounds as shown in figure. This polymeric type C8 column delivers complete baseline separation of structurally similar compounds such as vitamins D and D which is illustrated in figure. Inertsil ODS-P columns are also ideal for the HPLC analysis of 6 PAH compounds, listed as target pollutants by the U.S. EPA. Figure : Classification of Inertsil ODS phases by Standard Reference Material 869 Surface of Silica gel Planar Molecule Octadecyl Group Non-Planar Molecule Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.6 mm I.D. : A) CHCN B) HO A/B = 85/5, v/v :.0 ml/min : 0 C : UV 5 nm Polymeric α = k TBN/k BaP = 0.7 α : Polymeric-like Intermediate α =.7 < α <.7 : Intermediate Monomeric α =. α.7 : Monomeric-like. Phenanthro[,-c]phenanthrene (PhPh),. Tetrabenzonaphthalene (TBN) Inertsil ODS-P Inertsil ODS- Inertsil ODS-. Benzo[a]pyrene (BaP) Figure : Comparison of Selectivity between a Polymeric and Monomeric type C8 phase Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.6 mm I.D. : A) CHCN B) CHOH A/B = 75/5, v/v :.0 ml/min : 0 C : UV 80 nm HC CH HC CH CH CH HC HC HC CH CH Inertsil ODS-P Inertsil ODS- HO HO. Vitamin D. Vitamin D 06

37 Inertsil ODS-P Analytical : μm : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 07

38 Inertsil ODS-EP Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octadecyl Groups End-capping : None Carbon Loading : 9 % USP Code : L ph Range : ~ 7.5 PG : Polar Group Inertsil ODS-EP contains a polar functional group embedded between the silica surface and the C8 group. The embedded polar group makes the C8 phase stable in 00 % aqueous eluents without "phase collapse." This phase is also extremely "base deactivated" and provides superior peak shape for acids and bases in organic eluents as well as acidified eluents typically used in LC/MS. Figure : Comparison of Selectivity between various reversed phased columns,,, Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.6 mm I.D. : A) CHOH B) 0 mm KHPO(pH 7.0, KHPO) A/B = 50/50, v/v :.0 ml/min : 0 C : UV 0 nm OH NH. Benzyl alcohol. Aniline OH NO Inertsil ODS-EP Inertsil Ph- Inertsil C Phenol. Nitrobenzene Figure : Unique Selectivity of an Embedded Polar C8 Phase Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.0 mm I.D. : A) CHOH B) 0. % HCOOH in HO A/B = 50/50, v/v : 0. ml/min : 0 C : UV 5 nm Inertsil ODS-EP Inertsil Ph- Inertsil ODS- OH O OH O C C O. Phenoxyacetic acid. Benzoic acid 08

39 Inertsil ODS-EP Analytical : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 09

40 Inertsil ODS-80A Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 80 Å (8 nm) Pore Volume : 0.80 ml/g Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : 7.5 % USP Code : L ph Range : ~ 7.5 A relatively small pore size of 80 Å with high surface area silica delivers high number of theoretical plates for small molecule samples. GL Sciences' InertSustain C8 and Inertsil ODS- were a major advancement on the Inertsil ODS-80A columns, and generally provide superior chromatography and alternative selectivity to the Inertsil ODS-80A. We recommend InertSustain C8 or Inertsil ODS- columns for all new method development. Figure : Comparison With Inertsil ODS- Column Size : 5 μm, 50.6 mm I.D. Eluent : A) CHOH B) HO A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm. n-butylbenzene. o-terphenyl Guaranteed Number of Theoretical Plates Above 5,000 Inertsil ODS-80A[Pore Size 80 Å(8 nm)] Guaranteed Number of Theoretical Plates Above,000 Inertsil ODS-[Pore Size 50 Å(5 nm)]. n-amylbenzene. Triphenylene 00

41 Inertsil ODS-80A Analytical Length \ I.D : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm., , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 0

42 Inertsil ODS- Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 0 m /g Pore Size : 50 Å (5 nm) Pore Volume :.0 ml/g Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : 8.5 % USP Code : L ph Range : ~ 7.5 Inertsil ODS- columns have a pore size of 50 Å offering symmetric peaks for bases, acids with low pressure. When Inertsil ODS- was introduced in 987s, this HPLC transformed the entire industry. Inertsil ODS- was the first HPLC phase created using ultra high purity silica, which produced superior base deactivation. Until this phase was eclipsed by the performance of it s sibling Inertsil ODS-, it was GL Sciences most popular phase and continues to be used widely and reliably for long established methods in pharmaceutical and environmental labs. We recommend Inertsil ODS- columns for all new method development. Figure : Comparison of Retentivity with various Inertsil ODS Bonded Phases Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.6 mm I.D. : A) CHOH B) HO A/B = 70/0, v/v :.0 ml/min : 0 C : UV 5 nm,,. n-butylbenzene. o-terphenyl Inertsil ODS Inertsil ODS Inertsil ODS-SP. n-amylbenzene. Triphenylene Analytical Length \ I.D : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm., , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. 0

43 Inertsil ODS Physical Properties Silica : Spherical Silica Gel : 5 μm, 0 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.00 ml/g Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : % USP Code : L ph Range : ~ 7.5 Reversed Phase HILIC Inertsil ODS columns are general purpose, reversed phase C8 columns available in 5 μm and 0 μm particle sizes. It was the first ODS bonded phase introduced from GL Sciences back in 986. We recommend InertSustain C8 or Inertsil ODS- columns for all new method development. Figure : Analysis of Lidocain under a condition based on the 6th Edition Japanese Pharmacopeia H N O N. Lidocain. Benzophenone O Column : Inertsil ODS (0 μm, 50.0 mm I.D.) Eluent : A).88 g Sodium laulyl salfate in CHCN B) 0.0 M NaHPO (ph.0, HPO) A/B = 9/, v/v Flow Rate :. ml/min Col. Temp. : 5 C Detection : UV 5 nm Normal Phase SEC Ion Exchange Analytical Length \ I.D : 5 μm Length \ I.D : 0 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 0 μm 5 μm 0 μm., , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Application Specific Guard Preparative Capillary Applications Cat. No. Index 0

44 InertSustain C8 Physical Properties Silica : Newly Developed ES Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume : 0.85 ml/g Bonded Phase : Octyl Groups End-capping : Complete Carbon Loading : 8 % USP Code : L7 ph Range : ~ 0 IInertSustain C8 is an octyl group (C8) bonded column delivering the same extreme inertness to any type of compounds just like InertSustain C8, which enables rapid analysis of highly hydrophobic compounds delivering symmetric peaks at a wide range of ph. Figure : Comparison of Retentivity between InertSustain C8 and InertSustain C8 9.9 min min 6 Column Size : 5 μm, 50.6 mm I.D. Eluent : A)CHOH B)HO A/B = 80/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Sample :. Uracil. Toluene. Ethylbenzene. Propylbenzene 5. n-butylbenzene 6. n-amylbenzene InertSustain C InertSustain C8 Figure : Analysis of Losartan Potassium and Hydrochlorothiazide Tablets, Assay Test (Based on the Condition of United States Pharmacopeia 6-NF) Column Eluent Flow Rate Col.Temp. Detection InjectionVol. Sample : InertSustainC8(5 μm,50.0 mm I.D.) : A)CHCN/Buffer* = 7/9,v/v(Premix) B)CHCN A/B=00/0 min 9/8 6min 8/6 min 00/0 5min 00/0,v/v :.0mL/min : 5 C : UV80 nm(gl-750 UV Detector) : 0 μl :. Hydrochlorothiazide 0. mg/ml. Losartan Potassium 0. mg/ml Tailing factor of :.79 ( <.5) * Buffer :.5 g/l of monobasic potassium phosphate and.5 g/l of dibasic sodium phosphate n water

45 InertSustain C8 Analytical : μm Length \ I.D Length \ I.D HPSeries : μm MPa (500 Bar) End-fittings are /6 Waters-compatible. UHPLC compatible end-fittings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6. Length \ I.D : μm Length \ I.D Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

46 Inertsil C8- Physical Properties Silica : Series High Purity Silica Gel : μm, μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octyl Groups End-capping : Yes Carbon Loading : 5 % USP Code : L7 ph Range : ~ 7.5 Many chromatographers prefer a C8 column when an ODS phase shows excessive retention values. Inertsil C8- provides the same separation pattern (selectivity) and extreme inertness to any type of compounds just like Inertsil ODS-, which enables easy method transfer from ODS- to C8- while other commercially available ODS and C8 columns can show dramatically different selectivity even though they are part of the same brand/series. Figure : Comparison of Separation Pattern between ODS and C8 7 7,, 7 ODS Time (min) Inertsil ODS XBridge C Luna C8() C8,, Time (min) Inertsil C XBridge C Luna C8() Column Size : 5 μm, 50.6 mm I.D. Eluent : A) CHOH B) HO A / B = 80/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Injection Vol. : 5 μl. Uracil (0.005 mg/ml). Caffeine (0.0 mg/ml). Phenol (0.08 mg/ml). n-butylbenzene (. mg/ml) 5. o-terphenyl (0.0 mg/ml) 6. n-amylbenzene (.7 mg/ml) 7. Triphenylene (0.0 mg/ml) 06

47 Inertsil C8- Analytical : μm Length \ I.D Length \ I.D HPSeries : μm MPa (500 Bar) End-fittings are /6 Waters-compatible. UHPLC compatible end-fittings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6. Length \ I.D : μm Length \ I.D Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 07

48 Inertsil C8- Physical Properties Silica : Series High Purity Silica Gel : μm, μm, 5 μm, 0 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octyl Groups End-capping : Yes Carbon Loading : 9 % USP Code : L7 ph Range : ~ 7.5 The same base silica gel and bonding technology that is used for Inertsil ODS- is also employed for Inertsil C8-. The difference between the two phases is just the length of the hydrocarbon ligands. As shown in figure, 0 μm particle size columns are also available to meet the requirement of various pharmacopeia methods. We recommend InertSustain C8 columns for all new method development. Figure : Comparison of Retentivity between Inertsil ODS- and Inertsil C8-5 5 Column Size: 5 μm, 50.6 mm I.D. Eluent : A) CHCN B) HO A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Sample :. Naphthalene. Ethylbenzene. n-propylbenzene. Anthracene 5. n-butylbenzene Inertsil C Inertsil ODS- Figure : Analysis of Losartan Potassium and Hydrochlorothiazide Tablets, Dissolution Test (Based on the Condition of United States Pharmacopeia 6-NF) Column : Inertsil C8- (0 μm, 50.6 mm I.D.) Eluent : A) CHCN B) 0 mm KHPO (ph.5, HPO) A/B = 0/60, v/v Flow Rate :. ml/min Col. Temp. : 5 C Detection : UV 0 nm Injection Vol. : 0 μl Sample :. Hydrochlorothiazide ( mg/l). Losartan potassium (60 mg/l) Time (min) 08

49 Inertsil C8- Analytical : μm Length \ I.D Length \ I.D HPSeries : μm MPa (500 Bar) End-fittings are /6 Waters-compatible. UHPLC compatible end-fittings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6. Reversed Phase HILIC Normal Phase Length \ I.D : μm Length \ I.D Length \ I.D : 5 μm Length \ I.D Length \ I.D..6 : 0 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

50 Inertsil C8 Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 0 m /g Pore Size : 50 Å (5 nm) Pore Volume :.0 ml/g Bonded Phase : Octyl Groups End-capping : Yes Carbon Loading : 0.5 % USP Code : L ph Range : ~ 7.5 Inertsil C8 columns have a pore size of 50 Å and it is recommended for rapid analysis of highly hydrophobic compounds. We recommend InertSustain C8 or Inertsil C8- columns for all new method development. Analytical Length \ I.D : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm., , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. 00

51 Inertsil C Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 0 m /g Pore Size : 50 Å (5 nm) Pore Volume :.0 ml/g Bonded Phase : Butyl Groups End-capping : Yes Carbon Loading : 7.5 % USP Code : L6 ph Range : ~ 7.5 Reversed Phase HILIC Inertsil C columns have a pore size of 50 Å and is recommended for rapid analysis of highly hydrophobic compounds such as fat-soluble vitamins. Figure : Comparison of Retentivity between Inertsil C and Inertsil C8 Column Size : 5 μm, 50.6 mm I.D. Eluent : CHCN Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 70 nm Sample :. Vitamin K (MK-). Vitamin K. Vitamin K (MK-7) Normal Phase SEC Inertsil C Inertsil C8 Ion Exchange Analytical Length \ I.D : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm., , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Application Specific Guard Preparative Capillary Applications Cat. No. Index 0

52 InertSustain Phenyl Physical Properties Silica : Newly Developed ES Silica Gel : μm, μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume : 0.85 ml/g Bonded Phase : Phenyl Groups End-capping : None Carbon Loading : 0 % USP Code : L ph Range : ~ 7.5 InertSustain Phenyl delivers an extremely unique reverse phase characteristics that are critical to resolving compounds that could not be separated on a C8 or C8 phase. InertSustain Phenyl provides not only pi-pi interactions, but also hydrogen bonding secondary interactions, which results in retaining polar compounds at the same time. As the phenyl groups are directly bonded to the silica gel, InertSustain Phenyl is compatible with the analysis of structural isomers due to it s high stereo-selectivity (figure ) while other alkyl phenyl type columns fails to separate. Figure : Comparison of Selectivity between InertSustain Phenyl and InertSustain C8, InertSustain Phenyl InertSustain C8 Column Size : 5 μm, 50.6 mm I.D. Eluent : A) CHOH B) HO A/B = 70/0, v/v Flow Rate : 0.8 ml/min Col. Temp. :0 C Detection : UV 5 nm. Butylbenzene. o -Terphenyl. Amylbenzene. Triphenylene Non - Planar Structure Planar Structure Figure : Analysis of Structural Isomers (Positional Isomers of Cresol),, Not Separated Not Separated InertSustain Phenyl Ascentis Phenyl XBridge Phenyl Column Size : 5 μm, 50.6 mm I.D. Eluent : A)CHOH B)HO A/B = 0/80, v/v Col. Temp. : 0 C Flow Rate : 0.8 ml/min Detection : UV 5 nm OH OH OH CH CH CH. o-cresol. m-cresol. p-cresol 0

53 InertSustain Phenyl Analytical : μm Length \ I.D Length \ I.D HPSeries : μm MPa (500 Bar) End-fittings are /6 Waters-compatible. UHPLC compatible end-fittings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6. Reversed Phase HILIC Normal Phase Length \ I.D : μm Length \ I.D Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. 0 SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

54 InertSustain Phenylhexyl Physical Properties Silica : Newly Developed ES Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume : 0.85 ml/g Bonded Phase : Phenylhexyl Groups End-capping : Yes Carbon Loading : 9.0 % USP Code : L ph Range :.0 ~ 0.0 InertSustain Phenylhexyl columns are bonded with phenylhexyl groups, which employs a phenyl ring with a hexyl (6- carbon) linker and is densely bonded to our newly developed ES silica gel delivering complementary selectivity to straight alkyl-chain columns, but with industry leading inertness, lot-to-lot reproducibility and low back pressure. Figure : Comparison of Selectivity with other brands, 7, , InertSustain Phenylhexyl Column Size : 5 μm, 50.6 mm I.D. Eluent : A) CHOH B) HO A/B = 70/0, v/v Flow Rate :.0 ml / min Col. Temp. : 0 C Detection : UV 5 nm Sample :. Uracil. Caffeine. Phenol. Butylbenzene 5. o-terphenyl 6. Amylbenzene 7. Triphenylene XBridge Phenyl Luna Phenyl-Hexyl Figure : Analysis of Acidic Compounds InertSustain Phenylhexyl XBridge Phenyl Luna Phenyl-Hexyl Column Size : 5 μm, 50.6 mm I.D. Eluent : A) CHCN B) 0.% HPO A/B = 5/75, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Sample :. Brilliant Blue FCF. Phenol. Salicylic acid

55 InertSustain Phenylhexyl Analytical Length \ I.D HP Series : μm Mpa(500 Bar) End-fi ttings are /6 Waters-compatible. UHPLC compatible end-fi ttings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fi ttings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6. Length \ I.D : μm Length \ I.D Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 05

56 Inertsil Ph- Physical Properties Silica : Series High Purity Silica Gel : μm, μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Phenyl Groups End-capping : None Carbon Loading : 9.5 % USP Code : L ph Range : ~ 7.5 Just like InertSustain Phenyl, Inertsil Ph- have phenyl groups directly bonded to the silica gel which provides pure reverse phase characteristics that are critical to resolving highly polar compounds like acidic and basic pharmaceuticals. The near perfect phenyl phase coverage on this material results in symmetric, narrow peaks for even the most polar compounds while using simple eluents like aqueous acetonitrile or methanol. Figure : Comparison of Selectivity with Inertsil ODS-, Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.6 mm I.D. : A) CHOH B) HO A/B = 90/0, v/v (ODS-) A/B = 70/0, v/v (Ph-) :.0 ml/min : 0 C : UV 5 nm. Fluorene Inertsil Ph Inertsil ODS-. Phenanthrene Figure : Analysis of Aromatic Hydrocarbons with various Bonded Phases 5,,5 5 Column : 5 μm, 50.6 mm I.D. Eluent : A) CHCN B) HO A/B = 50/50, v/v (Phenyl columns) A) CHCN B) HO A/B = 70/50, v/v (ODS) Flow Rate : ml/min Col. Temp. : 0 C Detection : UV 5 nm CH5 (CH) CH. Ethylbenzene. Naphthalene. n-propyl benzene Inertsil Ph- (Phenyl Group) Inertsil Ph (Alkyl Phenyl Group) Inertsil ODS- (Alkyl Group) (CH) CH. n-butylbenzene 5. Anthracene 06

57 Inertsil Ph- Analytical : μm Length \ I.D Length \ I.D HPSeries : μm MPa (500 Bar) End-fittings are /6 Waters-compatible. UHPLC compatible end-fittings are also available upon request for UHPLC systems (Ex: UPLC) to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a.6 mm I.D. column) For maximum operating pressure information, please refer to page 6. Length \ I.D : μm Length \ I.D Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 07

58 Inertsil Ph Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 0 m /g Pore Size : 50 Å (5 nm) Pore Volume :.0 ml/g Bonded Phase : Phenethyl Groups End-capping : Yes Carbon Loading : 0 % USP Code : L ph Range : ~ 7.5 Inertsil Ph has phenethyl groups bonded to silica gel which offers weak pi-pi interactions. As it is modified with phenethyl groups, hydrophobic interactions between alkyl chain and analytes play an important role in separation as well as pi-pi interactions. To change the selectivity or elution pattern drastically, InertSustain Phenyl is recommended as it provides strong pi-pi interactions, resulting in resolving compounds that could not be separated on a C8 or C8 phase. Figure : Comparison of Selectivity with GL Sciences Phenyl columns Inertsil Ph Inertsil Ph- InertSustain Phenyl Column Size : 5 μm, 50.6 mm I.D. Sample : Eluent Flow Rate Col. Temp. Detection : A) CHOH B) HO A/B = 60/0, v/v :.0 ml/min : 0 C : UV 5 nm. Uracil. Ethylbenzene. Naphthalene. Propylbenzene 5. Butylbenzene 6. Anthracene Figure : Comparison of Selectivity between other brand's Phenyl column, 5, Inertsil Ph Inertsil Ph- other brand s Phenyl Column Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.6 mm I.D. : A) CHCN B) HO A/B = 50/50, v/v :.0 ml/min : 0 C : UV 5 nm CHCH. Ethylbenzene (CH)CH. n-buthylbenzene. Naphthalene 5. Anthracene (CH)CH. n-propylbenzene 08

59 Inertsil Ph Analytical Length \ I.D : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm., , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 09

60 Inertsil CN- Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Cyanopropryl Groups End-capping : None Carbon Loading : % USP Code : L0 ph Range : ~ 7.5 Inertsil CN- is a cyanopropyl bonded phase that provides pi-pi interactions offered by the triple bond in cyano groups. Due to its chemistry, separation can be achieved for those compounds that could not be separated on a straight-chain-alkyl columns such as C8 or C8 bonded phases (Figure. ). Figure illustrates the relative retention of each compound to n-propylbenzene. Although Inertsil ODS-EP and Inertsil Ph- has the ability to recognize the electron-state, each column delivers different elution pattern. Inertsil CN- column can be used for both reversed phase and normal phase separations. The column is originally shipped in a mixture of n-hexane/ ethanol. When using the column for reversed phase applications, fully equilibrate the column before use. Figure : Comparison of Recognition of Electronic-State with Inertsil ODS-, Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.6 mm I.D. : 0 mm KHPO(pH.0, HPO) :.0 ml/min : 0 C : UV 0 nm COOH COOH Inertsil CN Inertsil ODS- HN C CH H CONH HN C CH H COOH. Asparagine. Aspartic acid H COOH H H C C C C HOOC H HOOC COOH. Fumaric acid. Maleic acid Figure : Comparison of Recognition of Electronic-State between various Inertsil columns Relative Retention Inertsil ODS-EP Inertsil Ph- Inertsil CN- Relative Retention to n-propylbenzene ) n-butylbenzene ) Naphthalene 5) Anthracene Alkylbenzene Polyaromatic Inertsil ODS-EP Inertsil Ph- Inertsil CN- 5 Column Size : 5 μm, 50.6 mm I.D. Eluent Flow Rate Col. Temp. Detection Sample : A) CHCN B) HO A/B = 70/0, v/v (ODS-EP) A/B = 0/60, v/v (Ph-, CN-) :.0 ml/min : 0 C : UV 5 nm :. Ethylbenzene. Naphthalene. n-propylbenzene. n-butylbenzene 5. Anthracene 00

61 Inertsil CN- Analytical : μm : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 0

62 Inertsil WP00 C8 Physical Properties Silica : WP00 Series High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : 9 % USP Code : L ph Range : ~ 7.5 Inertsil WP00 C8 (wide pore size of 00 Å) columns bring the same legendary performance of Inertsil's narrow-pore HPLC products to columns designed specifically for the reproducible separations of proteins and peptides. The results of GL Sciences original end-capping technique are shown in figure, which provide high efficiency and good peak shape for proteins and peptides. Figure : Comparison with other brands Inertsil WP00 C other brand s 00 C8 Column B Column Size Eluent other brand s 00 C8 Column A : 5 μm, 50.6 mm I.D. : A) 0.05 % HCOOH in (CHCN/HO = 90/0, v/v) B) 0.05 % HCOOH in HO A/B = 0/90 60 min 0/60, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm other brand s 00 C8 Column C Sample :. Methionine Enkephalin (Tyr-Gly-Gly-Phe-Met, M.W. 57). Oxytocin (Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH, M.W.,007). Angiotensin Ⅱ (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe, M.W.,0). Leucin Enkephalin (Tyr-Gly-Gly-Phe-Leu, M.W. 556) 5. Angiotensin Ⅰ (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, M.W.,97) 6. Insulin (M.W. 6,000) 7. Insulin Chain B (M.W.,96) Figure : Analysis of Proteins mau Column : Inertsil WP00 C8 (5 μm, 50.0 mm I.D.) Eluent : A) 0. % TFA in CHCN B) 0. % TFA in HO A/B = 0/80 0 min 70/0, v/v Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : UV 80 nm Injection Vol. : 0 μl Sample :. Ribonuclease B. Cytochrome C. Lysozyme. BSA 5. Ovalbumin

63 Inertsil WP00 C8 Analytical : μm : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 0

64 Inertsil WP00 C8 Physical Properties Silica : WP00 Series High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octcyl Groups End-capping : Yes Carbon Loading : % USP Code : L7 ph Range : ~ 7.5 Inertsil WP00 C8 (wide pore size of 00 Å) columns bring the same legendary performance of Inertsil's narrow-pore HPLC products to columns designed specifically for the reproducible rapid separations of proteins and peptides. As shown in figure, Inertsil WP00 C8 delivers rapid analysis with sharper peaks compared to Inertsil WP00 C8. Figure : Comparison with Inertsil WP00 C Inertsil WP00 C8 Inertsil WP00 C8 Column Size Eluent Flow Rate Col. Temp. Detection Sample : 5 μm, 50.6 mm I.D. : A) CHCN/0.05 % TFA in HO = 80/0, v/v B) CHCN/0.05 % TFA in HO = 0/90, v/v A/B = 0/00 0 min 00/0 0 min hold, v/v :.0 ml/min : 0 C : UV 80 nm :. DL-Phenylalanine (M.W. 65). Cytochrome C (M.W.,000). Lysozyme (M.W.,000). BSA (M.W. 66,000) 5. α-chymotrypsinogen A (M.W. 5,600) 6. Ovalbumin (M.W. 5,000) Analytical Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm , , , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. 0

65 Inertsil WP00 C Physical Properties Silica : WP00 Series High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Butyl Groups End-capping : None Carbon Loading : % USP Code : L6 ph Range : ~ 7.5 PG : Polar Group Reversed Phase HILIC Inertsil WP00 C is a butyl group bonded phase utilizing silica gel with wide pores (00 Å). Also, an optimal polar group is embedded between silica surface and butyl group, which reduces adsorption of basic compounds and fat-soluble proteins. Inertsil WP00 C is recommended for the analysis of large, highly fat-soluble proteins and peptides as illustrated in figure. Figure : Comparison of Performance between other brand's 00 C column Inertsil WP00 C Analytical 9 5, Commercially Available 00 C Column A 7 : 5 μm, 50.6 mm I.D. Column Size Eluent Flow Rate Col. Temp. Detection Sample : A) 0. % HCOOH in (CHCN/HO = 90/0, v/v) B) 0. % HCOOH in HO A/B = 0/80 0 min 80/0, v/v :.0 ml/min : 0 C : UV 80 nm :. Neurotensin (M.W.,67). Leucin Enkephalin (M.W. 556). Cytochrome C (M.W.,000). Insulin (M.W. 6,000) 5. BSA (M.W. 66,000) 6. Myoglobin (M.W. 7,000) 7. Creatine amidinohydrolase (M.W.,000) 8. Ovalbumin (M.W. 5,000) 9. Creatinine amidohydrolase (M.W. 70,000) Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 05

66 HPLC Information Operating Pressure of Maximum Operating Pressure of Analytical Analytical Maximum Operating Pressure (MPa) InertSustainSwift C8.9 μm 80 InertSustain, Inertsil μm 80 * InertSustain, Inertsil μm HP 50 InertSustain, Inertsil μm, μm, 5 μm, 0 μm 0 Unisil 5 μm, 0 μm 0 InertSphere Sugar- 5 μm 5 Bioptic AV-, AV- 5 μm 5 Capillary EX μm, 5 μm 0 Capillary EX-Nano μm, 5 μm 5 The maximum operating pressure of a 50 mm length column or shorter is 50 MPa for Inertsil ODS-, C8-, Ph-. Maximum Operating Pressure of Guard Guard Maximum Operating Pressure (MPa) Cartridge Guard Column E 0 Cartridge Guard Column Ei 0 Guard Column for UHPLC 80 SILFILTER STD C8 5 Capillary Micro Guard 0 06

67 HILIC ---- Inertsil Amide 08 Inertsil HILIC 050 InertSustain NH 05 Inertsil NH 05 07

68 Inertsil Amide Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Carbamoyl Groups End-capping : None Carbon Loading : 8 % USP Code : None ph Range : ~ 7.5 Inertsil Amide column is bonded of carbamoyl group, and it shows strong retention of highly polar compounds. As shown below, compare to other commercial columns, Inertsil Amide showed high retentivity. Inertsil Amide provides excellent performance for those hard to retain compounds using an ODS column with a long lifetime. Figure : Comparasion with other brands Inertsil HILIC Atrantis HILIC 5 6 ZIC HILIC Column Size : 5 μm, 50.0 or. mm I.D. Eluent : A) CHCN B)0 mm HCOONH A/B = 90/0, v/v Flow Rate : 0. ml/min Col.Temp. : 5 C Detection : UV 5 nm 7 8 O O NH NH. Toluene N H O N H. Thymine. Uracil O 5 6 TSKgel Amide HO O N O NH O HO N N O HN N N NH N OHOH OHOH N H O. Uridine 5. Adenosine 6. Cytosine NH O 5 6 Inertsil Amide 7 8 HO N O N O HO N N O N NH NH OHOH OHOH Time (min) 7. Cytidine 8. Guanosine 08

69 Inertsil Amide Analytical : μm : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 09

70 Inertsil HILIC Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Diol Groups (Dihydroxypropyl Groups) End-capping : None Carbon Loading : 0 % USP Code : L0 ph Range : ~ 7.5 HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. (figure.) Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when organic concentration is raised, it shows a strong retention (figure.). Moreover, it can get more stable analysis by adding basic solvent to the eluate, such as ammonium acetate. Figure : Separation mode from Reverse phase to HILIC ODS column Reverse phase mode ODS column ODS column HILIC column HILIC column ph control Using Ion pair reagent Using higher organic solvent Add buffer No retention No retention No retention Improve to a stable condition. Allantoin Reproducibility of adjust eluent first time nd time rd time Column : ODS Eluent : A) CHCN B) HO A/B = 5/95, v/v Column : ODS Eluent : A) CHCN B) 0 mm phosphate buffer(ph 7.5) A/B = 5/95, v/v Column : ODS Eluent : A) CHCN B) IPCC-0 A/B = 5/95, v/v Column : Inertsil HILIC Eluent : A) CHCN B) HO A/B = 95/5, v/v Column : Inertsil HILIC Eluent : A) CHCN B) 0 mm CHCOONH A/B = 95/5, v/v Figure : Correlation between retention time and the concentration of CH CN in mobile phase 95 % CHCN 85 % CHCN 75 % CHCN Column Eluent Flow Rate Col. Temp. Detection Sample : Inertsil HILIC (5 μm, 50.0 mm I.D.) : A) CHCN B) 0 mm CHCOONH : 0. ml/min : 0 C : UV 0 nm :. Allantoin 50 % CHCN HN O H N NH 5 % CHCN O NH O 050

71 Inertsil HILIC Analytical : μm : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 05

72 InertSustain NH Physical Properties Silica : Newly Developed ES Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume : 0.85 ml/g Bonded Phase : Aminopropyl Groups End-capping : None Carbon Loading : 7 % USP Code : L8 ph Range : ~ 7.5 InertSustain NH shows far superior stability compared to other brand available aminopropyl columns, as our newly developed Evolved Surface Silica is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of sugars or vitamin E. However, the shift in retention time has been an issue for a long time. InertSustain NH delivers highly reliable reproducibility and stability with accurate qualitative and quantitative results. Further more, aminopropyl columns generally can not be washed by weakly acidic eluent, however InertSustain NH was improved and it can be washed by weakly acidic eluent. Figure : Comparison of preventing anomer resolution of sugar analysis Eluent : A) CHCN B) HO A/B = 75/5 Flow Rate :.0 ml/min, v/v Col. Temp. : 0 C Detection : RI Sample :. Fructose ( mg/ml). Glucose ( mg/ml). Sucrose ( mg/ml). Maltose (50 mg/ml) InertSustain NH other brand Amide Column Figure : Comparison of stability between various Amino column Figure : Reliable reproducibility 05 InertSustain NH 00 Other Brands NH Column A Other Brands NH Column B Initial retention time (%) Lot. 80 Lot Column Size : 5 μm, 50.6 mm I.D. Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : RI Volume of eluent passing through the column (ml) Sample:. Maltose Column Size : 5 μm, 50.6 mm I.D. Eluent : A)CHCN B)HO A/B = 85/5, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : RI Sample :. Fructose. Glucose. Sucrose. Moltose 05

73 InertSustain NH Analytical : μm : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 05

74 Inertsil NH Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Aminopropyl Groups End-capping : None Carbon Loading : 8 % USP Code : L8 ph Range : ~ 7.5 IInertsil NH column is chemically bonded with Aminopropyl group. It is widely used for analyzing sugar in reversed phase mode. Compare to InertSustain NH, InertSustain NH provides a better performance on durability. However, compare to other brand amiopropyl columns, Inertsil NH still shows excellent and strong retentivity. Figure : Comparision of Aminopropyl columns Inertsil NH InertSustain NH Column Size Eluent Flow Rate Col. Temp. Detection Sample : 5 μm, 50.6 mm I.D. : A) CHCN B) HO A/B = 75/5, v/v :.0 ml/min : 0 C : RI :. Fructose. Glucose. Sucrose. Maltose 05

75 Inertsil NH Analytical : μm : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 055

76 056

77 Normal Phase ---- Inertsil Diol 058 Inertsil SIL-00A 060 InertSustain NH 06 Inertsil NH 06 Inertsil CN- 066 Inertsil SIL-50A 068 Inertsil WP00 SIL

78 Inertsil Diol Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Diol Groups (Dihydroxypropyl Groups) End-capping : None Carbon Loading : 0 % USP Code : L0 ph Range : ~ 7.5 Inertsil Diol has characteristics of dihydroxypropyl group bonded phase. It shows unique selectivity normal phase mode. The separation mechanism of diol column is featured by hydrogen bonding interactions between diol groups and polar compounds. Diol columns provide an alternative selectivity to silica columns often with increased retentivity. Figure, selectivities of Inertsil Diol and Inertsil SIL-00A(a pure Silica gel column) are compared. Inertsil Diol shows higher selectivity for those compounds Figure, 9 compounds are eluted by Inertsil Diol and other normal phase columns of Inertsil series. By comparing their retention times of each compound, it is noticeable that Inertsil Diol provides stable retention for all of the compounds, including basic and acidic compounds. As nonspecific adsorption of water is reduced, Inertsil Diol can be washed by 00 % water eluent. Figure : Comparison of selectivity between Diol column and Silica column 5 Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.6 mm I.D. : A) n-hexane B) Ethanol A/B = 85/5, v/v :.0 ml/min : 0 C : UV 5 nm Inertsil Diol Sample : H O HO H O OH, 5 H H H H O O. Progesterone. Corticosterone O H O OH OH O H O OH OH HO O H H OH OH Inertsil SIL-00A O H H H H H H O O. Cortisone. Prednisone 5. Prednisolone Figure : Selectivity of Inertsil Diol Column : Inertsil Diol (5 μm, 50.0 mm I.D.) Eluent : A) n-hexane/ethanol = 00/, v/v B) Ethanol A/B = 00/0 0 min 5/75, v/v Flow Rate : 0. ml/min Col. Temp. Detection : 0 C : UV 5 nm 6, 7 HCO HO N NH OH. Eugenol. Pyridine. Aniline. Benzyl alcohol SIL-00A Diol NH CN- OH 5. Phenol OH 6. α-naphthol N N HC H N CH N O N CH O 7. Carbazole 8. Caffeine HO OH 9. Resorcinol Retention 058

79 Inertsil Diol Analytical : μm : 5 μm Length \ I.D..0.5 End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 059

80 Inertsil SIL-00A Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : None End-capping : None Carbon Loading : - % USP Code : L ph Range : ~ 7.5 Inertsil SIL-00A is a pure silica gel column available in normal phase mode. Because of the high quality of its silica gel, Inertsil SIL-00A achievs separation with sharp peaks and provides high column-to-column reproducibility. This excellent silica gel ideally designed for HPLC is the basis for Inertsil -series of GL Scineces. GL Sciences is the first company which emphasized the importance of silica-gel purity and determined the nature of the silanol impurities in the Silica gel. GL Sciences has established a successful manufacturing process for ultra pure silica gel with smooth and rigid surface. The SEM photos of Inertsil SIL-00A and other brands silica gel are shown as figure. Particles of Inertsil SIL-00A stand out by the smooth surface, uniformity in size and spherical shape. From figure, we can know as silanols on the silica surface interact with basic compounds, Inertsil SIL-00A retains basic compounds strongly and acidic compounds weakly. Figure : SEM photos of Inertsil SIL-00A and other brand available Silica gels Inertsil SIL-00A Silica gel A Silica gel B Silica gel C Silica gel D Figure : Selectivity of Inertsil TM SIL-00A Column Eluent : Inertsil SIL-00A (5 μm, 50.0 mm I.D.) : A) n-hexane/ethanol = 00/, v/v B) Ethanol A/B = 00/0 0 min 5/75, v/v Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : UV 5 nm HCO HO N NH. Eugenol. Pyridine. Aniline SIL-00A Diol NH CN- 6 OH. Benzyl alcohol OH 5. Phenol OH 6. α-naphthol N N HC H N CH N O N O CH 7. Carbazole 8. Caffeine HO OH 9. Resorcinol Retention 060

81 Inertsil SIL-00A Analytical : μm : 5 μm Length \ I.D..0.5 End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 06

82 InertSustain NH Physical Properties Silica : Series Newly Developed ES Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume : 0.85 ml/g Bonded Phase : Aminopropyl Groups End-capping : None Carbon Loading : 7 % USP Code : L8 ph Range : ~ 7.5 InertSustain NH is a newly developed ES silica column, compare to the other normal silica columns, InertSustain NH performs a sharp peaks, and high reproducible results with exceptional stability and durability that will maintain performance over the lifetime of the method. Also, InertSustain NH is an easily equilibrate column. Figure is an analysis sample to show the equilibrate time of InertSustain NH column. Using an column was replaced with isopropanol, phthalic acid ester as the sample and measured the time until stabilized the analysis conditions. From the chromatogram we can know at the total flow time of minutes of th injection, the column was stabled to be analyze. Figure : Analysis of Vitamin E with InertSustain NH & Inertsil NH column. α-tocopherol. β-tocopherol. γ-tocopherol. δ-tocopherol FLU InertSustain NH FLU Inertsil NH R= Column Eluent Flow Rate Col. Temp. Detection Injection Vol. Sample : 5 μm, 50.6 mm I.D. : A) n-hexane B) Ethyl acetate A/B = 85/5, v/v :. ml/min : 0 C : FL Ex. 98 nm Em. 5 nm : 0 μl : α-tocopherol ( μg/ml in Hexane) β-tocopherol ( μg/ml in Hexane) γ-tocopherol ( μg/ml in Hexane) δ-tocopherol ( μg/ml in Hexane) (C6H) Figure : Evaluation of equilibration time st injection (0 min) 0 6 Di-n-propylphthlate nd injection (7 min) 0 6 rd injection ( min) 0 6 Diethy phthalate th injection ( min) 0 6 Column Eluent Flow Rate Col. Temp. Detection : InertSustain NH (5 μm, 50.6 mm I.D.) : A) n-hexane/isopropanol = 00/, v/v B) Isopropanol A/B = 95/5, v/v (gradient mixer) :.0 ml/min : 0 C : UV 00 nm 5th injection (8 min)

83 InertSustain NH Analytical : μm : 5 μm Length \ I.D Length \ I.D Length \ I.D Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 06

84 Inertsil NH Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Aminopropyl Groups End-capping : None Carbon Loading : 8 % USP Code : L8 ph Range : ~ 7.5 Inertsil NH has a wide surface area, and bonded with aminopropyl groups, an amino column performs high retention time. Inertsil NH achieves better separation than other commercially available Amino columns as it is modified with Primary Amines. Figure shows that Inertsil NH is able to separate isomers of Tocopherol with good peak shape in short time compared to other commercially Amino columns. Primary Amines on the surface provides Inertsil NH unique selectivity as a normal phase column. Inertsil NH retains acidic compounds strongly and basic compound weakly. (Figure ) Figure : Analysis of Vitamin E HO HO HO HO O. α-tocopherol R O. β-tocopherol R O. γ-tocopherol R O. δ-tocopherol R R= (C 6 H ) FLU Inertsil NH 0 FLU InertSustain NH Column Eluent Flow Rate Col. Temp. Detection Injection. Vol. Sample : 5 μm, 50.6 mm I.D. : A) n-hexane B) Isopropanol A/B = 98/, v/v :. ml/min : 0 C : FL Ex. 98 nm Em. 5 nm : 0 μl : α-tocopherol ( μg/ml in Hexane) β-tocopherol ( μg/ml in Hexane) γ-tocopherol ( μg/ml in Hexane) δ-tocopherol ( μg/ml in Hexane) Figure : Selectivity of Inertsil NH Column Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.0 mm I.D. : A) Hexane/Ethanol = 00/, v/v B) Ethanol A/B = 00/0 0 min 5/75, v/v : 0. ml/min : 0 C : UV 5 nm H CO HO N NH. Eugenol. Pyridine. Aniline SIL-00A Diol NH CN- OH. Benzyl alcohol,7 6 OH 5. Phenol OH 6. α-naphthol H N 7. Carbazole N N H C HO CH N O N O CH OH 8. Caffeine 9. Resorcinol Retention 06

85 Inertsil NH Analytical : μm : 5 μm Length \ I.D Length \ I.D Length \ I.D Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 065

86 Inertsil CN- Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Cyanopropryl Groups End-capping : None Carbon Loading : % USP Code : L0 ph Range : ~ 7.5 Inertsil CN-, Cyanopropryl groups bonded to Silica gel with high density. And it is not only increase difference recognition of hydrophilicity, but also increase the durability. It is difficult to increase the both performance with other previous cyanopropryl columns. Inertsil CN- can be cleaned with 00 % aqueous solvent because non-specific adsorption of water effected with silanol base is prevented. Figure shows 9 compounds analysis with Inertsil CN-. And also it shows different selectivity of the other Inertsil series columns. Each compounds are detected adequate retentivity. And then they are easy to use as same as Inertsil Diol on normal phase mode. Regarding the reversed phase mode, please refer to page 0. Figure : Comparison of selectivity between Inertsil CN- and Commercially available Cyano Column : Cyano Column (5 μm, 50.6 mm I.D.) Eluent : A) Hexane B) Ethanol A/B = 90/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm O H O OH OH HO H H O OH OH Time (min) Time (min) Time (min) Inertsil CN- TSKgel CN-80TS Capcell Pak CN SG0 8 O H H H H O. Prednisone. Prednisolone Figure : Selectivity of Inertsil CN- Column Eluent Flow Rate Col. Temp. Detection : Inertsil CN- (5 μm, 50.0 mm I.D.) : A) Hexane/Ethanol = 00/, v/v B) Ethanol A/B = 00/0 0 min 5/75, v/v : 0. ml/min : 0 C : UV 5 nm H CO HO N NH. Eugenol. Pyridine. Aniline SIL-00A Diol NH CN- 6 OH. Benzyl alcohol OH 5. Phenol 5,7 OH 6. α-naphthol 8 9 H C N N H N CH N O N O CH 7. Carbazole 8. Caffeine HO OH 9. Resorcinol Retention 066

87 Inertsil CN- Analytical : μm : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 067

88 Inertsil SIL-50A Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 0 m /g Pore Size : 50 Å (5 nm) Pore Volume :.0 ml/g Bonded Phase : None End-capping : None Carbon Loading : - % USP Code : L ph Range : ~ 7.5 Inertsil SIL-50A is ultra pure silica gel column, and this ultra pure silica gel contains very low level of metal impurities and is durable and free from dents and cracks which can cause premature column failure. Compared to Inertsil SIL-00A, the silica s surface area is smaller (0 m²/ g). Retentivity of Inertsil SIL-50A is weaker than that of Inertsil SIL-00A. (Figure ) Figure : Comparison of retentivity and selectivity between Inertsil SIL-50A and Inertsil SIL-00A 7 6,5 9 8 HCO HO N. Eugenol. Pyridine Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, 50.0 mm I.D. : A) n-hexane/ethanol = 00/, v/v B) Ethanol A/B = 00/0 0 min 5/75, v/v : 0. ml/min : 0 C : UV 5 nm Inertsil SIL-50A (50 Å) 6 NH OH. Aniline. Benzyl alcohol H N OH OH 5. Phenol 6. α-naphthol N N HC HO CH N O N CH O OH 8. Caffeine 7. Carbazole 9. Resorcinol Inertsil SIL-00A (00 Å) Analytical Length \ I.D : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm., , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page

89 Inertsil WP00 SIL Physical Properties Silica : High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : None End-capping : None Carbon Loading : - % USP Code : L ph Range : ~ 7.5 Reversed Phase HILIC Inertsil WP00 SIL is pure silica gel phase with wide pores(00 Å). It is available for analysing compounds including large molecules. As the pore becomes wider, the surface area of silica gel is smaller. Since the interactions between the analyte and silica gel occur on the silica surface, smaller surface area means less interactions and faster elution. In the figure below, Inertsil WP00 SIL and Inertsil SIL-00A are compared to see their separation and eluting speed. The pore size of Inertsil SIL-00A is 00 Å and the surface area is 50 m²/g. As shown, Inertsil WP00 SIL elutes faster than Inertsil SIL-00A though their separating patterns are similar. Normal Phase Figure : Comparison of selectivity and retentivity between Silica gel columns with different pore size. 7 6, Inertsil WP00 SIL (00 Å) 8 HCO HO N NH OH. Eugenol. Pyridine. Aniline. Benzyl alcohol Column Size : 5 μm, 50.0 mm I.D. Eluent Flow Rate Col. Temp. Detection : A) n-hexane/ethanol = 00/, v/v B) Ethanol A/B = 00/0 0 min 5/75. v/v : 0. ml/min : 0 C : UV 5 nm SEC Ion Exchange Inertsil SIL-00A (00 Å) Analytical 8 H N OH OH 5. Phenol 6. α-naphthol 7. Carbazole HC HO N N CH N O N O CH OH 8. Caffeine 9. Resorcinol Length \ I.D : 5 μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm , , , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page Application Specific Guard Preparative Capillary Applications Cat. No. Index

90 070

91 SEC ---- Inertsil Diol 07 Inertsil WP00 Diol 07 07

92 Inertsil Diol Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Diol Groups (Dihydroxypropyl Groups) End-capping : None Carbon Loading : 0 % USP Code : L0 ph Range : ~ 7.5 Inertsil Diol, dihydroxypropyl groups chemical bonded onto silica gel, can be used for water group SEC(GFC) or organic solvents SEC(GPC). (Figure ). As features of the packing material, it can analyze with several columns in series since 0 Mpa as the maximum operating pressure, and it is higher than polymer base columns. Figure shows an example of polystyrene analysis with Inertsil Diol and Inertsil WP00 Diol in series. With coupling columns in different pore size in series, it can be used for broader range of molecular weight compared with figure calibration curve. General internal diameter of SEC columns are 7-8 mm. But even if.6 mmi.d. column, it can obtain calibration curve with smaller elution volume rather than 7.6 mm I.D. column. Therefore it can be analyzed with saving solvent, environment conservation, and low cost. Figure : Calibration curve for aqueous and organic SEC analysis Molecular Weight Inertsil Diol Inertsil WP00 Diol M.W.. 6, , ,000. 8, , , , ,000 9., Molecular Weight Inertsil Diol Elution Capacity (ml) Calibration curve for polystyrenes M.W..,000, ,000. 0,000. 0, , ,000 7.,000 8., Elution Capacity(mL) Calibration curve for polystyrene glycol Column : Inertsil WP00 Diol (5 μm, mm I.D.) Eluent : THF Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Column : Inertsil Diol (5 μm, mm I.D.) Eluent : HO Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : RI (Cell Temp. 5 C) Figure : Calibration curve for small I.D. SEC columns Molecular Weight Inertsil WP00 Diol + Inertsil Diol 8 M.W.. 6, , ,000. 8, , , ,00 8., (No.9 is propyl bemzene) Molecular Weight Inertsil WP00 Diol + Inertsil Diol 8 M.W.. 6, , ,000. 8, , , ,00 8., (No.9 is propyl bemzene) mm I.D. 9.6 mm I.D Elution Capacity (ml) Calibration curve for polystyrene with 7.6 mm I.D. column Column : Inertsil WP00 Diol (5 μm, mm I.D.) + Inertsil Diol (5 μm, mm I.D.) Eluent : THF Flow Rate :.0 ml/min Col. Temp. : 5 C Detection : UV 5 nm Elution Capacity (ml) Calibration curve for polystyrene with.6 mm I.D. column Column : Inertsil WP00 Diol (5 μm, 50.6 mm I.D.) + Inertsil Diol (5 μm, 50.6 mm I.D.) Eluent : THF Flow Rate : 0. ml/min Col. Temp. : 5 C Detection : UV 5 nm 07

93 Inertsil Diol Analytical : μm : 5 μm Length \ I.D Length \ I.D Length \ I.D Length \ I.D Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For the guard column I.D. over 6.0 mm.please refer to page 5. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 07

94 Inertsil WP00 Diol Physical Properties Silica : WP00 Series High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Diol Groups (Dihydroxypropyl Groups) End-capping : None Carbon Loading : 9 % USP Code : L0, L ph Range : ~ 7.5 Inertsil WP00 Diol has dihydroxypropyl group bonded to silica gel with pore size 00 Å and is capable of analyzing large molecules. Like Inertsil Diol, Inertsil WP00 Diol can be used for both aqueous SEC (Size Exclusion Chromatography) and organic SEC. Also, as a diol column, Inertsil WP00 Diol can be used in both normal phase and reversed phase mode. As the pore size of silica gel is wider than that of Inertsil Diol, Inertsil WP00 Diol is capable of separating compounds with a broader range of molecular weight than Inertsil Diol. (Figure.) Figure : Calibration curve and analysis of polystyrenes Molecular Weight Inertsil WP00 Diol M.W.. 6, , ,000. 8, , , , ,000 9., Column Size Eluent Flow Rate Col. Temp. Detection : 5 μm, mm I.D. : THF :.0 ml/min : 0 C : UV 5 nm 6 8 M.W.. 70,000 (0 μg/ml). 8,000 (0 μg/ml) 6. 9,000 (0 μg/ml) 8. 5,000 (0 μg/ml). 580 (0 μg/ml) 000 Inertsil Diol Elution capacity (ml) Calibration curve for polystyrenes by organic SEC Analysis of polystyrenes by Inertsil WP00 Diol Figure : Calibration curve and analysis of proteins Molecular Weight M.W.. Thyroglobulin 660,000. Ferritin,000. γ-globulins 60,000. Phosphorylase 97,00 5. Transferrin, human 80, BSA 66, Ovalbumin 5, Trypsinogen 5, Lysozyme,00 0. Insulin 6,000. Insulin chain B,500 Column : Inertsil WP00 Diol (5 μm, mm I.D.) Eluent : 0. M NaCl, 0. M NaHPO (ph 6.7, NaHPO) Flow Rate :.0 ml/min Col. Temp. : 5 C Detection : UV 80 nm Injection. Vol. : 0 μl Ferritin (0 μg/ml) 5. Transferrin, human (60 μg/ml) 8. Trypsinogen (60 μg/ml) 9. Lysozyme (80 μg/ml) 0. Insulin (60 μg/ml) Elution capacity (ml) Calibration curve for proteins Analysis of proteins by Inertsil WP00 Diol 07

95 Inertsil WP00 Diol Analytical : 5 μm Length \ I.D Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For the guard column I.D. over 6.0 mm.please refer to page 5. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 075

96 076

97 Ion Exchange ---- Inertsil AX 078 Inertsil CX

98 Inertsil AX Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Diethylaminopropyl Groups End-capping : None Carbon Loading : 7 % AEC : 0. meq/g USP Code : - ph Range : ~ 7.5 Inertsil AX has diethylamino groups bonded to silica gel by an alkyl chain. The diethylamino groups offer anionic functions required for anion exchange chromatography. It is mainly used for analyses of acidic compounds. Conventional ion-exchange columns used to show inconsistent results from lot to lot. However, Inertsil AX is manufactured under strict quality control in order to offer excellent lot-to-lot reproducibility. The retentivity of Inertsil AX is influenced by the concentration of buffer. The retention time can be adjusted by the concentration of buffer (Refer to Fig. ). Figure : Comparison of lot-to-lot reproducibility with other brands Lot. A Lot. A Column Size : 5 μm, 50.6 mm I.D. Eluent : 60 mm KHPO (ph.0, HPO) Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Injection Vol. Sample : μl :. Cytidine 5 -monophosphate (CMP). Uridine 5 -monophosphate (UMP) NH O Lot. B Lot. B O N O NH Lot. C Lot. C HO P O O O N O HO P O O O N O 0 Inertsil AX other brand s ion-exchange column OH OH Cytidine 5 -monophosphate (CMP) OH OH Uridine 5 -monophosphate (UMP) Figure : Effect of buffer concentration in eluent Eluent : 0 mm KHPO (ph.0, HPO) 5 Eluent : 0 mm KHPO (ph.0, HPO) 5 Column Flow Rate Col. Temp. Detection Injection Vol. Sample : Inertsil AX (5 μm, 50.6 mm I.D.) :.0 ml/min : 0 C : UV 5 nm : 0 μl :. Cytidine 5 -monophosphate (CMP). Adenine 5 -monophosphate (AMP). Uridine 5 -monophosphate (UMP). Guanosine 5 -monophosphate (GMP) 5. Xanthosine 5 -monophosphate (XMP) Eluent : 0 mm KHPO (ph.0, HPO)

99 Inertsil AX Analytical : 5 μm Length \ I.D Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 079

100 Inertsil CX Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Benzensulfonyl Groups End-capping : None Carbon Loading : % CEC : 0.5 meq/g USP Code : L9 ph Range : ~ 7.5 Inertsil CX has benzenesulfonyl groups bonded to silica gel by an alkyl chain. The sulfony groups at the end of the structure offer cationic functions required for the cation exchange chromatography. It is mainly used for analyses of basic compounds. Inertsil CX is manufactured under strict quality control in order to offer excellent lot-to-lot reproducibility as the same as Inertsil AX. Inertsil CX has high ion exchange capacity and provides high retentivity and selectivity. Therefore, it is also suited for analyzing amino acids and nucleobases shown in Fig. and Fig. Figure : Comparison of lot-to-lot reproducibility with other brands CEC : 0.5 meq/g CEC : 0. meq/g Column Size : 5 μm, 50.6 mm I.D. Eluent : 0. M CHCOONH (ph 5.0, HCOOH) Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 60 nm Injection vol. Sample : 0 μl :. Uracil (0.0 mg/ml). Thymine (0.0 mg/ml). Cytosine (0.0 mg/ml). Adenine (0.0 mg/ml) Inertsil CX Comitial Cation-Exchange column Figure : Biogenic Amine Analysis Figure : Nucleoside Analysis COOH OH N HO HO NH H N OH N O HO NH. DL-DOPA. Creatinine. DL-Noradrenalin OH HO HO O NH O NH N O N N O H H H N NH. Uracil. Thymine. Guanine N O NH NH NH HO HO NH HO. DL-Adrenaline 5. Dopamine hydrochoride 6. Tyramine 5 NH N N NH N 5 6 N N N O H H. Cytosine 5. Adenine Column : Inertsil CX (5 μm, 50.6 mm I.D.) Eluent : 0. M KHPO (ph 6.0, KHPO) Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Injection Vol. : 0 μl Sample :. DL-DOPA (0.98 mg/ml). Creatinine (0. mg/ml). DL-Noradrenalin (.0 mg/ml). DL-Adrenaline (.05 mg/ml) 5. Dopamine hydrochloride (0.99 mg/ml) 6. Tyramine (.0 mg/ml) 0 0 Column : Inertsil CX (5 μm, 50.6 mm I.D.) Eluent : 0. M HCOONH (ph 5.0, HCOOH) Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 60 nm Injection Vol. : 0 μl 0 Sample :. Uracil (0.05 mg/ml). Thymine (0.05 mg/ml). Guanine (0.5 mg/ml). Cytosine (0.5 mg/ml) 5. Adenine (0.5 mg/ml) 080

101 Inertsil CX Analytical : 5 μm Length \ I.D Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.0 Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm , , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 08

102 08

103 Application Specific ---- Bioptic AV-, AV- 08 Inertsil Peptides C8 085 Inertsil Acrolein C8 085 InertSphere Sugar- 086 Inertsil Sulfa C8 087 Inertsil AS 087 Corresponding Pharmacopeia (JP, USP, EP) 088 Corresponding SFC

104 Application Specific Bioptic AV-, AV- Physical Properties Silica : Spherical shape : 5 μm Bonded Phase : Avidin Temperature Range : ~ 0 deg.c ph Range : ~ 7.5 Max. concentration of organic solvent For AV- ; < 0 % For AV- ; No restriction Feature Glycoprotein bonded silica Protein elutes as a void volume peak Large sample capacity Analysis of drug moving state Simplified method by using Bioptic columns Existing Method Drug compounds in Serum of plasma Denaturing, Racemization risk Structural alteration of compounds Switch to Normal phase solvent (In case of using the normal phase colum) Protein precipitation Dilution or Switch solvent Filtration Method using Bioptic columns Filtration Simple and easymethod available Bioptic AV- Chiral separation capability Chiral separation in a reversed phase mode Bioptic AV- Compatible with high concentrations of organic solvents Similar retention behavior as reversed phase mode Existing Chiral column Existing Reverse phase column Bioptic AV- Chiral separation available Bioptic AV- For reverse phase separation Analytical : 5 μm Length 50 I.D. End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable.5,. Length Bioptic AV- Bioptic AV- 5 μm 5 μm I.D. Replacement Cartridge E Guard Column ( EA.) Bioptic AV- Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) Replacement Cartridge E Guard Column ( EA.) Bioptic AV- Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) 5 μm 5 μm 5 μm 5 μm , , , , Holder for Cartridge Guard Column E End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. For 0 mm Length For 0 mm Length

105 Application Specific Inertsil Peptides C8 Physical Properties Silica : Series High Purity Silica Gel : μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g The whole manufacturing process; synthesis of silica gel, chemical modification, packing, quality test, is under the strict quality control. The number of theoretical plates is as many as 00,000 plates/m. For peptide mapping, analytical result of standard peptides obtained by each lot is attached to the column. For protein analysis, Inertsil WP00 or Inertsil WP00 C8 is recommended. Analytical Length \ I.D : μm Length \ I.D End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Inertsil Acrolein C8 Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm μm , , , , , Holder for Cartridge Guard Column E For 0 mm Length For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Inertsil Acrolein offers rapid separation of DNPH-Acetone and DNPH-Acrolein under a general mobile phase condition such as Acetonitrile / Water. Analytical Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : 5 % USP Code : L ph Range : ~7.5 Bonded Phase : Octadecyl Groups End-capping : Yes Carbon Loading : 9 % USP Code : L ph Range : ~7.5 Length I.D. Cat.No. : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

106 Application Specific InertSphere TM Sugar- Physical Properties Silica : Styrene Divinylbenzene copolymerization Particle size : 5 μm Exchange capacity : 0.7 meq/g Organic solvent resist : 0 ~ 00 % ( Methanol only ) Bonded Phase : Quaternary Alkylamine ph Range : ~ InertSphere Sugar- is a suitable anion-exchange column for sugar analysis. It is packed quaternary ammonium group binding polymer. High sensitivity sugar analysis is available using an electrochemical detector ED7. Especially InertSphere Sugar- is suitable for Monosaccharide and Disaccharide analysis. Note ; Solvent Bottle with CO Trap Cartridge is necessary for analysis to avoid dissolving carbonate ion in the solvent. The Solvent Bottle CO Trap Cartridge contains hazardous material which requires special freight handling. Additional charges apply. Analytical Length I.D. Cat.No. : 5μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cat.No. Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) Cat.No End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page

107 Application Specific Inertsil Sulfa C8 Physical Properties Silica : Series High Purity Silica Gel : μm, 5 μm Surface Area : 50 m²/g Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octadecyl End-capping : Yes Carbon Loading : 5 % USP Code : L As drug residues in food has became a major problem today, developing analytical methods of synthetic bacterial drugs including Sulfa drugs is important. Inertsil Sulfa C8 is a superb ODS column designed for analysis of sulfa drugs. Each lot of Inertsil Sulfa C8 is tested for the effective separation of sulfa drugs and will be delivered to you with its annalistic data. Reversed Phase HILIC Analytical Length \ I.D : μm Length \ I.D : 5 μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) μm 5 μm μm 5 μm., , , , For 0 mm Length Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Inertsil AS Physical Properties Silica : Series High Purity Silica Gel : 5 μm Surface Area : 50 m²/g Inertsil AS is for analysis of Arsenic compounds which are Toxic compounds in environment water. As an Arsenic speciation analysis column, simultaneous analysis of Arsenic compounds is available with HPLC/ICP-MS. Analytical Length \ I.D.. : μm End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Cartridge Guard Column E I.D. of the Analytical Column Applicable. Length I.D. Pore Size : 00 Å (0 nm) Pore Volume :.05 ml/g Bonded Phase : Octadecyl Replacement Cartridge E Guard Column ( EA.) Cat.No. End-capping : Yes Carbon Loading : 5 % USP Code : L Cartridge E Holder / Cartridge Set ( Cartridge E Guard & Holder) Cat.No Holder for Cartridge Guard Column E For 0 mm Length End-fittings are /6 Waters-compatible. For maximum operating pressure information, please refer to page 6. Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 087

108 Corresponding Pharmacopeia (JP, USP, EP) GL Sciences offers varies particle sizes and lengths corresponding to Japanese Pharmacopeias (JP), US Pharmacopeias (USP) or European Pharmacopeias (EP). 5 μm HPLC Packing material Column I.D. Column length Description Cat. No. InertSustain C mm Inertsil ODS Inertsil ODS mm Inertsil WP00 C InertSustain C mm Inertsil ODS Inertsil ODS C8(ODS) InertSustainSwift C mm Inertsil ODS-SP mm Inertsil WP00 C InertSustain C mm Inertsil ODS Inertsil ODS InertSustain C mm 00 mm Inertsil ODS Inertsil ODS mm 60 mm Inertsil C InertSustain C mm 50 mm Inertsil C Inertsil C C8 Inertsil WP00 C InertSustain C mm 80 mm Inertsil C Inertsil C mm Inertsil C Pre-column.0 mm 5 mm InertSustain C InertSustain C Please contact us for other sizes of columns. Figure : Crospovidone Figure : Clopidogrel sulfate Column : InertSustain C8 (5 μm, 50.0 mm I.D.) Sample : Guard Column : InertSustain C8 (5 μm, 5.0 mm I.D.). -Vinyl--pyrrolidone Eluent : A) CHCN B) HO A/B = /9, v/v. Vinyl acetate Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Injection Vol. : 50 μl Data Source : LC InertSearch No. LB Column : InertSustain C8 (5 μm, 50.9 mm I.D.) Eluent : A) 0.87 g/l IPCC-05 in HO (ph.5, HPO) /CHOH = 9/, v/v (IPCC-05: Sodium - Pentanesulfonate) B) CHCN/CHOH = 9/, v/v A/B = 60/0, v/v Flow Rate :.5 ml/min Col. Temp. : 0 C Sample : Detection : UV 0 nm. p-hydroxybenzoic acid iso-propyl ester Injection Vol. : 0 μl ( mg/l) Data Source : LC InertSearch No. LB6. Clopidogrel sulfate ( mg/l) 088

109 Corresponding Pharmacopeia (JP, USP, EP) 7 μm HPLC Packing material Column I.D. Column length Description Cat. No..0 mm 50 mm Inertsil ODS mm Inertsil ODS mm Inertsil ODS C8(ODS) 5 mm Inertsil ODS mm 50 mm Inertsil ODS mm Inertsil ODS NH.6 mm 5 mm Inertsil NH μm HPLC Packing material Column I.D. Column length Description Cat. No. Inertsil ODS mm 00 mm Inertsil ODS mm Inertsil ODS C8(ODS).0 mm 00 mm.6 mm 00 mm 50 mm Inertsil ODS Inertsil ODS Inertsil ODS Inertsil ODS Inertsil ODS Reversed Phase HILIC Normal Phase SEC Other ODS(C8) Packing material Column I.D. Column length Description Cat. No. μm.6 mm mm Inertsil ODS-SP μm.0 mm 50 mm Inertsil WP00 C μm.9 mm 50 mm Inertsil ODS Please contact us for other sizes of columns. Pharmacopeias applications are available on page 7~8. Also many other applications are available at InertSearch and Technical Note on our website. InertSearch... LC Technical Note... Figure : Telmisartan Column : Inertsil ODS-SP (5 μm, 5.0 mm I.D.) Eluent : A) CHCN/CHOH = /, v/v B).0 g/l KHPO +. g/l IPCC-05 (ph.0, 0 % HPO in HO) (IPCC-05: Sodium -Pentanesulfonate) A/B = 0/70-5 min - 80/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 0 nm Injection Vol. : μl Data Source : LC InertSearch No. LB6 Sample :. Telmisartan Figure : Risperidone Column : Inertsil WP00 C8 (.5 μm, 50.0 mm I.D.) Eluent : A) CHCN B) HO C) THF A/B = 00/800/.5, v/v/v (ph.0, 8 % NH Solution) Flow Rate : 0.55 ml/min Col. Temp. : 5 C Detection : UV 75 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LB5 Sample :. Risperidone (00 mg/l) Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 089

110 Corresponding SFC Mainly supercritical CO is used as mobile phase in SFC (Supercritical Fluid Chromatography). It is said suitable for high speed analysis because of lower viscosity than the general HPLC mobile phase and fast diffusion speed is fast in mobile phase. It is exceptional method for preparative and purification purpose because almost mobile phase is volatilized when back to normal pressure. InertSustain and Inertsil series columns are available for SFC analysis. The packing materials are the same as HPLC columns but they are packed in stainless tubes which correspond to SFC. Various packing materials are available, and then utilize for expand the SFC application range. Example of SFC/MS Analysis Ten(0) kinds of pesticides are analyzed with Inertsil ODS-EP which contains a polar group embedded between the silica surface and Octadecyl group (C8) as the below. The separation patterns are different from polar group chemical bonded column like mainly used on SFC. Relative Abundance Column Size Eluent Flow Rate Col. Temp. Injection vol. Back Pressure : Inertsil ODS-EP (5 μm, 50.6 mm I.D.) : A) Supercritical carbon dioxide B) 0. % ammonium formate in methanol A/B = 95/5 - min - 95/5 - min - 90/0-0 min - 80/0 : ml/min : 5 C : 5 ml : 0 MPa Sample:. Methamidophos. Acetamiprid. Carbendazim. Dimethirimol 5. Emamectin benzoate (Ba) 6. Chlorfluazuron 7. Acequinocyl 8. Pyridaben 9. Cypermethrin 0. Etofenprox This data is provided by Prof. Dr. Akio Baba About packing materials InertSustain and Inertsil series are available. Please contact us the details. Inspected with HPLC only, not with SFC. Sizes The following four() sizes are available as standard SFC columns. Please describe the packing materials when you order. Description Cat.No. Corresponding SFC column 5 μm,. 50 mm Corresponding SFC column 5 μm,.6 50 mm Corresponding SFC column 5 μm, 0 50 mm Corresponding SFC column 5 μm, 0 50 mm Maximum operating column pressures are 8 MPa on. 50 mm and 5 MPa on.6 50 mm. End-fittings are /6 Waters-compatible only. 090

111 Guard ---- Guard Column s selection and Use 09 Cartridge Guard Column E 09 Cartridge Guard Column Ei (Non-metal type) 09 Guard for UHPLC 096 GL Cart 098 Packed Guard, Mini Guard 099 SILFILTER TM STD C8 0 Filters, Impurity Remove 05 09

112 Guard Column s Selection and Use Guard columns are installed between the injector and the analytical column of a HPLC system, mainly to protect analytical columns. Guard columns are widely used as a cost effective for prolong HPLC column life. Exchangeable cartridge design and unchangeable packing design are offered. Exchangeable cartridge design contains holder and cartridge column, and the cartridge columns are disposable, easy -to-use, and you can change the guard column in seconds. Packed design is packed as same as analytical column, using as a guard column. Guard column design Description Compatible analytical columns ID Guard columns length Cartridge Guard Column E Page 9 First choice for Guard Column.0~.5 mm 0 mm Cartridge Guard Column Ei Page 9 Guard column with PEEK inlet and outlet.~.6 mm 0, 0 mm Exchangeable Cartridge design UHPLC Guard Column Page 96 For high speed, high efficiency separations.0~.0 mm 0 mm SILFILTER STD C8 Page 0 Almightily for various ODS columns.0~.6 mm 0 mm GL-Cart Guard Column Page 98 Direct-Connection and Indirect- Connection designs with economic cost.0,.6 mm 5 mm Packed Guard Column Page 99 Packed as same as analytical column.0~.6 mm, 50 mm Unchangeable packed design Packed Mini Guard Column Page 0 Short length type of packed guard column.0,.6 mm 0 mm Preparative Guard Column Page Guard columns for preparative columns 6.0~00 mm 50 mm 75 mm(for 50 mm I.D.) 00 mm (for 00 mm I.D.) Selecting packing material When selecting the packing material for the guard columns, we recommend to choose the same with analytical columns. However, SILFILTER STD C8 is an monolithic guard column, it can fit different kinds of ODS columns. Selecting dimensions Particle size : To not alter the chromatography of the analytical column, the packing of the guard column should be the same as the analytical column. Inner diameter : Choose a guard column with an internal diameter similar to the analytical column internal diameter. Pre-column Coupler W (PCTFE) Connectors Universal self-adjusting connector Column Coupler, using for when connect guard columns and analytical column. Pre-column Coupler SUS 09

113 Cartridge Guard Column E Cartridge guard column E designed with an special holder and cartridge, universal selfadjusting connections, reusable by changing cartridges. Cartridges contain the same high performance packing material that are used in our GL Sciences analytical columns. Easy-to-use, convenient, and disposable guard columns can protect your more expensive analytical columns. Reversed Phase Line-up list InertSustain C8 I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder/Cartridge Set ( Cartridge E Guard Column & Holder) μm 5 μm μm 5 μm , , , , , Inertsil ODS- I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder/Cartridge Set ( Cartridge E Guard Column & Holder) μm 5 μm μm 5 μm , , , , , Inertsil ODS- I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder/Cartridge Set ( Cartridge E Guard Column & Holder) μm μm 5 μm μm μm 5 μm , , , , , Inertsil ODS- Cartridge Guard Column E I.D. of the Analytical Column Applicable Length I.D. Replacement Cartridge E Guard Column ( EA.) Cartridge E Holder/Cartridge Set ( Cartridge E Guard Column & Holder) 5 μm 5 μm , , , , , All the guard columns are Waters type end-fitting. Recommend Max. Operating Pressure is 0 MPa. Other packing material of cartridge guard columns E are available, please confirm page 5~87 of each analytical columns. HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 09

114 Cartridge Guard Column Ei (Non-metal type) Cartridge Guard column Ei is a special design for the separation which need to avoid the metal accessible surface. It is signed with a special PEEK holder and cartridge. * Length of Cartridge Guard column Ei is 0mm Cartridge Guard Column Ei (Non-metal type) InertSustain C8 I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil ODS- I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil ODS-SP I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil ODS-EP I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil C8- I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil ODS- I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil ODS- I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil ODS-P I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , InertSustain C8 I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil C8- I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , All Cartridge Guard column Ei designed with 0 mm length. All the guard columns are Waters type end-fitting. Recommend Max. Operating Pressure is 0 MPa. 09

115 Cartridge Guard Column Ei (Non-metal type) InertSustain Phenyl I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil CN- I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil Amide I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , InertSustain NH I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil Diol I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil WP00 Diol I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil Ph- I.D. of the Analytical Column Applicable I.D. All Cartridge Guard column Ei designed with 0 mm length. All the guard columns are Waters type end-fitting. Recommend Max. Operating Pressure is 0 MPa. Other packing material of cartridge guard columns Ei are available, please contact your local distributor for more information. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil WP00 C8 I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil HILIC I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil NH I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Inertsil SIL-00A I.D. of the Analytical Column Applicable I.D. Replacement Cartridge Ei Guard Column ( EA.) Cartridge Ei Holder/ Cartridge Set ( Cartridge Ei Guard Column & Holder) 5 μm 5 μm.0, , Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

116 Guard for UHPLC Holder for UHPLC Guard Column Replacement Cartridge Guard Column InertSustain C8 Length 0 Guide Packing Guard column for UHPLC significantly extends column lifetime of your analytical column. The replacement cartridge guard column can be exchanged easily. A /6 inch tube is connected at the outlet of the holder for easy installation with minimized dead volume. Specifications Silica : High Purity Silica Gel : μm, μm Maximum Pressure : 80 MPa Analytical column I.D. :.0 ~.0 mm I.D. Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set I.D. μm μm μm μm InertSustainSwift TM C8 Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set Length I.D..9 μm μm.9 μm μm Inertsil ODS- Length 0 Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set I.D. μm μm μm μm Inertsil ODS- Length 0 Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set I.D. μm μm μm μm Inertsil ODS-SP Length I.D. Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set μm μm

117 Guard for UHPLC InertSustain C8 Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set Length I.D. μm μm μm μm Inertsil C8- Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set Length I.D. μm μm μm μm Reversed Phase HILIC Normal Phase Inertsil C8- Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set Length I.D. μm μm μm μm InertSustain Phenyl Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set Length I.D. μm μm μm μm SEC Ion Exchange Application Specific Inertsil Ph- Length 0 Holder Description I.D. Replacement Cartridge Guard Column ( EA) Cartridge Guard column ( EA) + Holder ( EA) set μm μm μm μm Cat.No. Holder for UHPLC Guard Column The tube size at the holder is /6 inch O.D. 0.8 mmi.d. 0 mm length. Installation Outlet side of the injector /6 inch Female (No.0-UNF) Tubing tip length is. mm. Column side Purchase a proper joint. Joint Very High Pressure Re-useable Fitting Description Max. Pressure QTY Cat.No. VHP-00 7 MPa set VHP-0 7 MPa set The tube size at the holder is /6 inch O.D. 0.8 mmi.d. 0 mm length. VHP-00 VHP-0 Guard Preparative Capillary Applications Cat. No. Index 097

118 GL Cart GL Cart guard column are designed as economical type to protect your expensive analytical column. GL Cart is one dimension 5mmL.6mmI.D. (Compatible analytical columns ID is.0 mm,.6 mm.) GL Cart holder is reusable. GL Cart Packing material Particle size (μm) Cartridge guard column GL Cart 0 EA.(5.6 mm I.D.) Cat. No. GL Cart Set (5 GL Cart cartridge + holder ) Cat. No. InertSustain C Inertsil ODS Inertsil ODS Inertsil ODS Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP InertSustain C Inertsil C Inertsil C InertSustain Phenyl Inertsil Ph Inertsil CN Inertsil WP00 C Inertsil WP00 C Inertsil WP00 C Inertsil Amide Inertsil HILIC InertSustain NH Inertsil NH Inertsil Diol Inertsil SIL-00A Inertsil SIL-50A Inertsil WP00 SIL Inertsil WP00 Diol All the guard columns are Waters type end-fitting. Recommend Max. Operating Pressure is 0 MPa. Other packing material of GL Cart guard columns are available, please contact your local distributor for more information. 098

119 Packed Guard, Packed Mini Guard Packed guard column and Mini guard column are designed different with cartridges type, using a high pressure to pack it as a guard column, and also can sustain analytical column s performance. Packed guard column has length mm and 50 mm two types, and Mini guard column has length 0 mm. Reversed Phase Packed Guard Column Packed Mini Guard Column Packed Guard Packing material InertSustain C8 Inertsil ODS- Inertsil ODS- Inertsil ODS- Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP All the guard columns are Waters type end-fitting. Recommend Max. Operating Pressure is 0 MPa. Length mm Length 50 mm I.D. μm 5 μm μm 5 μm HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 099

120 Packed Guard, Packed Mini Guard Packed Guard columns Packing material InertSustain C8 Inertsil C8- Inertsil C8- Inertsil C8 Inertsil C InertSustain Phenyl Inertsil Ph- Inertsil Ph All the guard columns are Waters type end-fitting. Recommend Max. Operating Pressure is 0 MPa. Length mm Length 50 mm I.D. μm 5 μm μm 5 μm

121 Packed Guard, Packed Mini Guard Packed Guard columns Packing material Inertsil CN- Inertsil WP00 C8 Inertsil WP00 C8 Inertsil WP00 C Inertsil Amide Inertsil HILIC InertSustain NH Inertsil NH All the guard columns are Waters type end-fitting. Recommend Max. Operating Pressure is 0 MPa. Length mm Length 50 mm I.D. μm 5 μm μm 5 μm Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 0

122 Packed Guard, Packed Mini Guard Packed Guard columns Packing material Inertsil Diol Inertsil SIL-00A Inertsil SIL-50A Inertsil WP00 SIL Inertsil WP00 Diol All the guard columns are Waters type end-fitting. Recommend Max. Operating Pressure is 0 MPa. Length mm Length 50 mm I.D. μm 5 μm μm 5 μm

123 Packed Guard, Packed Mini Guard Packed Mini Guard columns Packing material (μm) I.D. Length Cat. No InertSustain C Inertsil ODS Inertsil ODS Inertsil ODS Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP Inertsil ODS-80A InertSustain C Inertsil C Inertsil C Inertsil C Inertsil C InertSustain Phenyl Inertsil Ph Inertsil Ph Inertsil CN Inertsil WP00 C Inertsil WP00 C Inertsil WP00 C Inertsil Amide Inertsil HILIC InertSustain NH Inertsil NH Inertsil Diol Inertsil SIL-00A Inertsil SIL-50A Inertsil WP00 SIL Inertsil WP00 Diol All the guard columns are Waters type end-fitting. Recommend Max. Operating Pressure is 0 MPa. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 0

124 SILFILTER TM STD C8 SILFILTER STD C8 is a guard column with cartridge size.0 mm I.D. 0 mm. The cartridge is packed with a monolithic silica, chemically bonded with a C8 and uniquely end-capped based on our 5 years of experience. SILFILTER STD C8 can be installed and used for all C8 columns available in the market without interfering and influencing the elution or separation patterns. Also, deterioration of the packing in the cartridge can be confirmed visually. Feature Can be installed for all C8 columns available in the market. Deterioration of the packing in the cartridge can be confirmed visually. Easy to replace the cartridges. Effectively removes particulate and strongly retained sample compounds Delivers the lowest dead-volume. Specification Base Material : High Purity Monolithic Silica End-capping : Yes Max.Operating Pressure : 5 MPa (50 Bar) ph Range : ~7.5 Bonded Phase : Octadecyl Groups Max.Temperature : 50 C Size : 0.0 mm I.D. Compatible analytical ID :.0~.6 mm I.D. No Sacrifice in Separation Efficiency The following test was conducted to confirm the efficiency of SILFILTER STD C8. As shown below, the efficiency didn t decrease even for those fast eluting samples when installing SILFILTER STD C8. Analytical C8 column only RT :. min N : 8,500 RT : 7.7 min N : 8,500 With SILFILTER STD C8 RT :. min N : 8,800 RT : 8.0 min N : 8,800 Column : C8 Column ( μm, 50.0 mm I.D.) Eluent : A) CHCN B) HO A/B = 65/5, v/v Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : UV 5 nm Sample Acetaminophen Benzene Toluene Naphthalene Set A (with tube) Set B (without tube) Item Cat.No. SILFILTER STD C8, Set A* Cartridges with Holder and Tubes SILFILTER STD C8, Set B * Cartridges with Holder, without Tubes SILFILTER(with Tubes) Holder for A type* SILFILTER(without Tubes) Holder for B type* Replaceable Cartridge, ea PEEK Tough Connector, 5 pcs * : Tube size is, /6 inch O.D. 0.8 mm I.D. 0 mml * : Tubing tip length is. mm PEEK Tough Connector 0

125 Filters, Impurity Remove Pre-Column Coupler Pre-column Coupler W (PCTFE) Pre-column Coupler SUS Precolumn Coupler is an easy-to-use tool to connect guard columns and analytical columns. Pre-column Coupler has two designs, one is using PCTFE material, except common organic solvents, acidic solvent and basic solvent also can be used. And another one is stainless design, it can be used as high pressure. Specification Max. operation pressure:.7 MPa (PCTFE), 80 MPa (stainless) Tube O.D.: /6 Item Material Connection Cat.No. Pre-column Coupler W PCTFE Waters type Pre-column Coupler SUS Stainless * Product Pre-column Coupler SUS doe not fixed with Ferrules, therefore columns with 0-UNF specification all can be used. Reversed Phase HILIC Normal Phase Pre-Column Filter Pre-column filter Connect with /6 fitting Filter φ 0.50 Specification Fit in tube O.D. : /6 Screw specification : 0-UNF Filter pore size : μm Max. operation pressure :. MPa(SUS).5 MPa(PEEK) Item Jacket material P/N Qty. (pc) Cat.No. Pre-column filter μm Stainless A Replacement pre-column filter μm A PEEK pre-column filter μm PEEK A Replacement PEEK pre-column filter μm A SEC Ion Exchange Application Specific Pre-Clean ORG Pre-clean ORG A guard column to install between the pump and injector, to remove the impurity in aqueous eluent. Protect analytical LC columns, and prolong analytical columns lifetime. Easy-to-replace. Also can be used as an in-line filter, semimicro columns are most appropriate for column clog. types for two different flow rates. Item I.D. Length Recommend flow rate (ml/min) Cat.No. Pre-clean ORG replacement cartridge ea ~0 ml/min Pre-clean ORG holder and cartridgexea ~0 ml/min Pre-clean ORG holder Pre-clean ORG semi replacement cartridge ea ~.0 ml/min Pre-clean ORG semi holder and cartridgexea ~.0 ml/min Pre-clean ORG semi holder Guard Preparative Capillary Applications Cat. No. Index 05

126 06

127 Preparative Choosing Preparative 08 Preparative 09 Guard for Preparative JET 7 Other Preparative 7 07

128 Choosing Preparative Relationship between Column I.D., Sample Loading Volume and Flow Rate In preparative operations, column internal diameter sizes from 6.0 to 00 mm are widely used. When the analytical conditions along with the column packing material and length were the same between the analytical and preparative run, nearly the same chromatograms can be achieved by simply adjusting the flow rate and sample loading volume in proportion to the column cross-section area. The following table illustrates the appropriate flow rate against each column internal diameter sizes. 5 μm, 50.6 mm I.D. Flow Rate :.0 ml/min Linear Velocity : mm/s 5 μm, 50 0 mm I.D. Flow Rate : 8.9 ml/min Linear Velocity : mm/s Column I.D. Scale-Up Factor Appropriate Flow Rate (ml/min)* Reversed/Normal SEC HILIC Chiral Phases.6 0.~ ~ ~ ~ ~ ~ ~ ~ ~ * * 7~7* 0* 00* : When the particle size of the packing material is a 5 μm. : When the particle size of the packing material is a 0 μm. : When the particle size of the packing material is a 0 μm. Remarks Determine and optimize the analytical separation using.6 mm I.D. analytical columns. Semi-preparative HPLC columns can be used in standard HPLC systems. Column I.D. sizes from mm are generallyused when scaling-up in SEC. Column I.D. sizes dominantly used in preparative HPLCresearches. Dedicated preparative HPLC systems are requiredas a wide flow rate range is required. Smooth and Easy Scale-Up from Analytical to Preparative Dimensions Generally, analytical scale columns are used initially to determine and optimize separation conditions between the target peak and unwanted contaminants with various bonded phases. Efficient scale-up of methods from analytical to preparative dimensions requires the use of a packing material offering identical selectivity, otherwise methods would not scale as expected. InertSustain and Inertsil preparative HPLC columns offer asymmetry factor of nearly.0 delivering symmetric peaks maintaining efficiency of approximately 0,000 (N) when the column length is a 50 mm. As shown below, the scalability of InertSustain and Inertsil preparative HPLC columns provides smooth, easy and highly efficient purification and isolation results..6 mm I.D. Asy =.0, N =, mm I.D. Asy =.0, N =, mm I.D. Asy =.0, N = 0,000 System Column Eluent : PLC 76 System : Inertsil ODS- (5 μm, 50 mm) : A) CHCN B) HO A/B = 65/5, v/v Flow Rate : Linear Velocityat mm/s Col. Temp. : 0 C Col. Pres. : Approx. 5.0 MPa Detection : UV 5 nm Sample :. Acetophenone. Benzene. Toluene. Naphthalene 0 mm I.D. Asy =.0, N = 0,000 O CH CH mm I.D. Asy =.0, N = 0,000. Acetophenone. Benzene. Toluene. Naphthalene 0 mm I.D. Asy =.0, N = 0,000 0 mm I.D. Asy =.07, N = 0, mm I.D. Asy =.08, N = 8,000 08

129 Preparative Preparative InertSustain and Inertsil preparative columns are available in a broad range of column sizes to answer and meet all demands in preparative chromatography. Reversed Phase Phase Length I.D. Cat.No. Cat.No. Cat.No. Cat.No InertSustain C μm Inertsil ODS μm Inertsil ODS μm Inertsil ODS μm Inertsil ODS-SP 5 μm Inertsil ODS-P 5 μm Inertsil ODS-EP 5 μm End-fittings are /6 Waters-compatible. The maximum operating pressure of 6.0 to 50 mm I.D. columns are 0 MPa, 00 Bar. The maximum operating pressure of 00 mm I.D. column is 0 MPa, 00 Bar. HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 09

130 Preparative Phase Length I.D. Cat.No. Cat.No. Cat.No. Cat.No Inertsil ODS-80A μm Inertsil ODS μm Inertsil ODS μm Inertsil ODS μm InertSustain C μm Inertsil C μm Inertsil C μm Inertsil C μm Inertsil C μm End-fittings are /6 Waters-compatible. The maximum operating pressure of 6.0 to 50 mm I.D. columns are 0 MPa, 00 Bar. The maximum operating pressure of 00 mm I.D. column is 0 MPa, 00 Bar. 0

131 Preparative Phase Length I.D. Cat.No. Cat.No. Cat.No. Cat.No InertSustain Phenyl μm Inertsil Ph μm Inertsil Ph μm Inertsil CN μm Inertsil WP00 C μm Inertsil WP00 C μm Inertsil WP00 C μm End-fittings are /6 Waters-compatible. The maximum operating pressure of 6.0 to 50 mm I.D. columns are 0 MPa, 00 Bar. The maximum operating pressure of 00 mm I.D. column is 0 MPa, 00 Bar. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

132 Preparative Phase Length I.D. Cat.No. Cat.No. Cat.No. Cat.No Inertsil Amide μm Inertsil HILIC μm InertSustain NH μm Inertsil NH μm Inertsil Diol μm Inertsil SIL-00A μm End-fittings are /6 Waters-compatible. The maximum operating pressure of 6.0 to 50 mm I.D. columns are 0 MPa, 00 Bar. The maximum operating pressure of 00 mm I.D. column is 0 MPa, 00 Bar.

133 Preparative Phase Length I.D. Cat.No. Cat.No. Cat.No. Cat.No Inertsil SIL-50A μm Inertsil WP00 SIL μm Inertsil WP00 Diol μm Inertsil Peptides C μm End-fittings are /6 Waters-compatible. The maximum operating pressure of 6.0 to 50 mm I.D. columns are 0 MPa, 00 Bar. The maximum operating pressure of 00 mm I.D. column is 0 MPa, 00 Bar. Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

134 Guard for Preparative GL Sciences highly recommends the use of guard columns to protect and maximize the lifetime of your InertSustain or Inertsil preparative columns. Guard for Preparative Phase InertSustain C8 Inertsil ODS- Inertsil ODS- Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP Inertsil ODS-80A Inertsil ODS- Particle Size 5 μm 5 μm 5 μm 0 μm 5 μm 5 μm 5 μm 5 μm 5 μm I.D. Length Cat.No Phase Inertsil ODS InertSustain C8 Inertsil C8- Inertsil C8- Inertsil C8 InertSustain Phenyl Inertsil Ph- Inertsil Ph Particle Size 5 μm 0 μm 5 μm 5 μm 5 μm 0 μm 5 μm 5 μm 5 μm 5 μm I.D. Length Cat.No End-fittings are /6 Waters-compatible. The maximum operating pressure of 6.0 to 50 mm I.D. columns are 0 MPa, 00 Bar. The maximum operating pressure of 00 mm I.D. column is 0 MPa, 00 Bar.

135 Guard for Preparative Phase Inertsil CN- Inertsil WP00 C8 Inertsil WP00 C8 Inertsil WP00 C Inertsil Amide Inertsil HILIC InertSustain NH Inertsil NH Particle Size 5 μm 5 μm 5 μm 5 μm 5 μm 5 μm 5 μm 5 μm I.D. Length Cat.No Phase Inertsil Diol Inertsil SIL-00A Inertsil SIL-50A Inertsil WP00 SIL Inertsil WP00 Diol Inertsil Peptides C8 Particle Size 5 μm 5 μm 5 μm 5 μm 5 μm μm I.D. Length Cat.No Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index End-fittings are /6 Waters-compatible. The maximum operating pressure of 6.0 to 50 mm I.D. columns are 0 MPa, 00 Bar. The maximum operating pressure of 00 mm I.D. column is 0 MPa, 00 Bar. 5

136 PREP Guard Cartridges PREP guard cartridges offer economic and effective protection for extending the lifetime of your InertSustain or Inertsil preparative columns. Protection available for 7.6, 0 and 0 mm I.D. preparative columns. PREP Guard Cartridges Phase Length I.D. Replacement Cartridge ( EA.) Cat. No. Holder/Replacement Cartridge Set ( Cartridges & Holder) Cat. No. InertSustain C Inertsil ODS Inertsil ODS Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP Inertsil ODS-80A Inertsil ODS Inertsil ODS InertSustain C Inertsil C Inertsil C Inertsil C Inertsil C InertSustain Phenyl Inertsil Ph Inertsil Ph Inertsil CN Inertsil WP00 C Inertsil WP00 C Inertsil WP00 C Inertsil Amide Inertsil HILIC InertSustain NH Inertsil NH Inertsil Diol Inertsil SIL-00A Inertsil SIL-50A Inertsil WP00 Diol Inertsil WP00 SIL Inertsil Peptides C Holder for PREP Guard Cartridges Description Cat. No. Holder for PREP Guard Cartridges *End-fittings are /6 Waters-compatible. *The above dedicated holder must be purchased once to use the PREP Guard Cartridges. 6

137 Other Preparative JET JET JET columns have been designed specifically to work with rapid purification and isolation for preparative HPLC offering 0 % higher efficiency compared to general preparative columns. Inertsil ODS- Phase End-fittings are /6 Waters-compatible. The maximum operating pressure is 0 MPa, 00 Bar. Length 50 I.D. Cat.No Reversed Phase HILIC Normal Phase Other Preparative Inertsil PREP Other preparative HPLC columns such as Inertsil Prep using a 0 μm packing material and cost-effective Econo Prep series preparative columns are available using 0 μm particle size packing materials. SEC Ion Exchange Application Specific Econo Prep Guard Preparative Capillary Applications Cat. No. Index 7

138 8

139 Capillary Capillary HPLC 0 Totally Porous Particle type Capillary HPLC Connection Kits for Totally Porous Particle type Capillary 6 Totally Porous Particle type Capillary Micro Guard 7 Monolithic Capillary HPLC (Available in South Asia, Middle East and Africa only) 8 MonoSpray TM 9

140 Capillary HPLC Capillary EX and Capillary EX-Nano HPLC columns are excellent in analyzing trace amounts of samples in proteomic and bioanalytical analysis with high sensitivity and high resolution. Capillary EX and Capillary EX-Nano columns are totally porous particle type columns, which the flow rate is generally set under 00 μlmin. MonoCap is another capillary column, however, uses the monolithic silica technology offering high throughput, high sensitivity and high resolution separation of peptides and protein digests. MonoCap Fast-Flow provides high throughput analysis at half of the operating pressure compared to totally porous particle type columns. MonoCap Nano-flow deliver extremely high sensitivity in LC/MS due to the optimization of mesopore and throughpore sizes. Electrospray emitter for ESI-LC/MS, MonoSpray offer minimized sample diffusion resulting in high sensitivity. MonoCap Trap columns are also available for on-line preconcentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection. The chart below illustrates the recommended use and flow rate ranges when using a 50 mm length column. The red circle indicates the linear velocity at mm/s. MonoCap High Resolution 000 Figure : Recommended Use and Flow Rate Ranges Flow Rate(μL/min) Totally Porous Particle type Capillary Column CapillaryEX-Nano I.D. 0.05, 0.075, 0., 0. mm CapillaryEX I.D. 0., 0.5, 0.7 mm 0.05 mm I.D mm I.D. 0. mm I.D. 0. mm I.D. 0. mm I.D. 0.5 mm I.D. 0.7 mm I.D. Low-Density Monolithic Capillary Column MonoCap C8 Fast-flow I.D. 0.05, 0.075, 0., 0. mm Fast 0.05 mm I.D. Fast mm I.D. Fast 0. mm I.D. Fast 0. mm I.D. Wide 0.5 mm I.D. MonoCap C8 WideBore I.D. 0.5 mm MonoCap C8 High Resolution I.D. 0., 0. mm High Resolution mm I.D. High Resolution mm I.D. High-Density Monolithic Capillary Column 0.05 mm I.D mm I.D. 0. mm I.D. 0. mm I.D. MonoCap C8 Nano-flow I.D. 0.05, 0.075, 0., 0. mm Monolithic Trap Column MonoCap C8 Trap Column I.D. 0.05, 0.075, 0. mm 0.05 mm I.D mm I.D. 0. mm I.D. Monolithic Sprayer for Nano ESI-LC/MS FS mm I.D. FS mm I.D. FS 0. mm I.D. MonoSpray FS I.D. 0.05, 0.075, 0. mm MonoSpray C8 I.D , 0. mm C mm I.D. C8 0. mm I.D Flow Rate(μL/min) 0

141 Totally Porous Particle type Capillary HPLC Capillary EX-Nano Phase InertSustain C8 Inertsil ODS- Inertsil ODS- I.D Capillary EX End-fittings are Valco /6 (0- UNF). Valco / (6-0 UNF) end-fittings can also be arranged upon request, indicate / when ordering. The maximum operating pressure of 0.05 to 0. mm I.D. columns are 5 MPa, 50 Bar. The maximum operating pressure of 0. to 0.7 mm I.D. columns are 0 MPa, 00 Bar. 0.7 with I.D. sizes of 0.05, 0.075, 0. and 0. mm are Capillary EX-Nano columns. I.D. sizes of 0., 0.5 and 0.7 mm are Capillary EX columns. Capillary EX-Nano columns introduces a fused silica capillary tube having a very smooth and clean inner surface resulting in high theoretical plates. Capillary EX columns employs the same column hardware used in analytical columns, which is very easy to use. Length50 mm Length50 mm Length50 mm (μm) Cat.No. Cat.No. Cat.No Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

142 Totally Porous Particle type Capillary HPLC Phase I.D. (μm) Length 50 mm Length 50 mm Length 50 mm Cat.No. Cat.No. Cat.No. Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP InertSustain C8 Inertsil C8- End-fittings are Valco /6 (0- UNF). Valco / (6-0 UNF) end-fittings can also be arranged upon request, indicate / when ordering. The maximum operating pressure of 0.05 to 0. mm I.D. columns are 5 MPa, 50 Bar. The maximum operating pressure of 0. to 0.7 mm I.D. columns are 0 MPa, 00 Bar

143 Totally Porous Particle type Capillary HPLC Inertsil C8- Inertsil WP00 C8 Inertsil WP00 C8 Inertsil WP00 C InertSustain Phenyl Inertsil Ph- Phase I.D. (μm) End-fittings are Valco /6 (0- UNF). Valco / (6-0 UNF) end-fittings can also be arranged upon request, indicate / when ordering. The maximum operating pressure of 0.05 to 0. mm I.D. columns are 5 MPa, 50 Bar. The maximum operating pressure of 0. to 0.7 mm I.D. columns are 0 MPa, 00 Bar. Length 50 mm Length 50 mm Length 50 mm Cat.No. Cat.No. Cat.No Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

144 Totally Porous Particle type Capillary HPLC Phase I.D. (μm) Length 50 mm Length 50 mm Length 50 mm Cat.No. Cat.No. Cat.No. Inertsil Amide Inertsil HILIC InertSustain NH Inertsil NH End-fittings are Valco /6 (0- UNF). Valco / (6-0 UNF) end-fittings can also be arranged upon request, indicate / when ordering. The maximum operating pressure of 0.05 to 0. mm I.D. columns are 5 MPa, 50 Bar. The maximum operating pressure of 0. to 0.7 mm I.D. columns are 0 MPa, 00 Bar

145 Totally Porous Particle type Capillary HPLC Inertsil CN- Inertsil Diol Inertsil SIL-00A Inertsil AX Inertsil CX Titansphere TiO Phase I.D. (μm) End-fittings are Valco /6 (0- UNF). Valco / (6-0 UNF) end-fittings can also be arranged upon request, indicate / when ordering. The maximum operating pressure of 0.05 to 0. mm I.D. columns are 5 MPa, 50 Bar. The maximum operating pressure of 0. to 0.7 mm I.D. columns are 0 MPa, 00 Bar. Length 50 mm Length 50 mm Length 50 mm Cat.No. Cat.No. Cat.No Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 5

146 Connection Kits for Totally Porous Particle type Capillary Connection Kit for Capillary EX (Top Image: Installed view, Bottom Image: Contents of Kit) Connection Kit for Capillary EX (0., 0.5, 0.7 I.D. mm) Contents of Kit Column Coupler 0 0. mm I.D. /6 O.D.Tubing (Both ends with male nuts including PEEK ferrules) Capillary Tubing Connector mm I.D mm O.D. Tubing (Male nut, PEEK ferrule, /6 O.D. PTFE with sleeve) PTFE Tubing 0 mm pcs /6 O.D. (O.D mm Connection for Capillary Tubing) Cat.No Connection Kit for Capillary EX-Nano (Top Image: Installed view, Bottom Image: Contents of Kit) Connection Kit for Capillary EX-Nano (0.05, 0.075, 0., 0. I.D. mm) Contents of Kit Column Coupler mm I.D. /6 O.D. Tubing (Both ends with male nuts including PEEK ferrules) Capillary Tubing Connector mm I.D mm O.D. Tubing mm I.D mm O.D. Tubing mm I.D mm O.D. Tubing (Male nut, PEEK ferrule, /6 O.D. PTFE with sleeve) PTFE Tubing 0 mm pcs /6 O.D. (O.D mm Connection for Capillary Tubing) Cat.No How To Connect Injector Inertsil ODS- 5 μm mm KE000 Capillary EX-Nano Column Capillary Tubing Connector Inertsil ODS- μm Capillary EX Column Capillary Flow Cell Column Coupler Enlarged View of PTFE Tubing PTFE Tubing 6

147 Totally Porous Particle type Capillary Micro Guard Capillary EX Micro Guard columns are available in mm length which are ideal for the use of sample preconcentration and sample cleanup. Non-metal hardwares are also available to eliminate metal contamination from the column hardware. Reversed Phase Capillary EX Micro Guard Wetted Part Phase I.D. Length (μm) Metal Non-Metal Cat.No. Cat.No. InertSustain C Inertsil ODS Inertsil ODS Inertsil ODS-SP Inertsil ODS-P Inertsil ODS-EP InertSustain C Inertsil C Inertsil C Inertsil WP00 C Inertsil WP00 C Inertsil WP00 C InertSustain Phenyl Inertsil Ph Inertsil WP00 C Inertsil WP00 C Inertsil WP00 C Inertsil Amide Inertsil HILIC InertSustain NH Inertsil NH Inertsil CN Inertsil Diol Inertsil SIL 00A Inertsil AX Inertsil CX Titanspher TiO End-fittings are /6 Waters-compatible. The maximum operating pressure is 0 MPa, 00 Bar. HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 7

148 Monolithic Capillary HPLC MonoCap Series Mesopore Structure of Monolithic Silica Monolithic Silica Gel Throughpore GL Sciences MonoCap series, created synthetically via sol-gel method, and an octadecyl silane chemically bonded, has a very uniform three dimensional structure that shows excellent reproducibility from batch-tobatch. The solid structure of GL Sciences monolithic silica eliminates the need for frits or filters at the ends of the column, thereby reducing dead volume that might otherwise lead to band broadening or sample recovery. The high porosity of our monolithic silica allows high flow rates to be used without loss of resolutionor creation of high operating pressure. An optimized balance of throughpores and mesopores provides the critically importantcombination of efficiency, separation speed, large volume sample-loading, and small volume sample-recovery. MonoCap High Resolution provide extremely high efficiency, delivering over 00,000 plates for a,000 mm length column. The High Resolution Ultra type deliver over 00,000 plates. The Fast-flow type is compatible with high flow rate analysis due to its low flow resistance. In addition, the equilibration time can be minimized further by setting the flow rate high. MonoCap Nano-flow is a high-density monolithic capillary column offering extremely high sensitivity in LC/MS due to the optimization of mesopore and throughpore sizes. MonoCap Trap columns have a relatively big throughpore, which are available for on-line preconcentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection. Physical Properties of MonoCap Series Description Monolithic Silica Skeleton Throughpore Mesopore Porosity Bonded Phase End-capping Max. Operating Pressure μm μm 5 nm 85 % Octadecyl Groups Yes MPa MonoCap C8 High Resolution 750 MonoCap C8 High Resolution 000 μm μm 5 nm 85 % Octadecyl Groups Yes 5 MPa MonoCap C8 High Resolution Ultra 000 μm μm nm 85 % Octadecyl Groups Yes 5 MPa MonoCap C8 Fast-flow μm μm 5 nm 85 % Octadecyl Groups Yes MPa MonoCap C8 Nano-flow MonoCap C8 WideBore High Purity Silica Gel μm μm μm μm nm nm 85 % 85 % Octadecyl Groups Octadecyl Groups Yes Yes MPa MPa MonoCap C8 Trap Column μm 5 μm nm 85 % Octadecyl Groups Yes 0 MPa MonoCap Amide μm μm 5 nm 85 % Carbamoyl Groups None MPa MonoCap SCX μm 5 μm nm 85 % Based on monolithic technology, Merck KGaA, Darmstadt, Germany. Benzenesulfonyl Groups None 0 MPa End-fittings of MonoCap Monolithic Capillary HPLC MonoCap C8 High Resolution 750 MonoCap C8 fast-flow MonoCap Nano-flow MonoCap C8 WideBore MonoCap Amide MonoCap SCX. Metal Hardwares End-fittings are Valco /6 (0- UNF). Valco / (6-0 UNF) end-fittings can also be arranged upon request, indicate / when ordering.. PEEK Hardwares /6 male nut, ferrule and PTFE sleeve are included. MonoCap C8 High Resolution 000 MonoCap C8 High Resolution Ultra 000 MonoCap HILIC-UP High Resolution 000 End-fittings are not included. The connection kits shown at page must be purchased separately once. 8

149 Monolithic Capillary HPLC MonoCap TM C8 High Resolution/MonoCap TM C8 High Resolution Ultra MonoCap High Resolution 000 Maximizing all the benefits and advantages of monolithic technology, MonoCap High Resolution and High Resolution Ultra are appropriate for the efficient separation of peptides and protein digests. The newly-introduced High Resolution Ultra type deliver over 00,000 plates. Reversed Phase HILIC Normal Phase PEEK Metal MonoCap High Resolution 750 Figure : Analysis of Tryptic Digests SEC x0 強度 ( ) 000 Number of Theoretical Plates : + 00,000 Ion Exchange Column : MonoCap C8 High Resolution 000 (000 mm 0. mm I.D) Trap column : MonoCap C8 Trap Column (50 mm mm I.D.) Eluent : A) 0. % HCOOH in CHCN B) 0. % HCOOH in HO A/B = 0/ min - 5/55, v/v MonoCap TM C8 High Resolution Ultra I.D. Length Cat.No A column stand is included. End-fittings are not included. The connection kits shown at page must be purchased separately once. MonoCap TM C8 High Resolution 000 I.D. Length Cat.No A column stand is included. End-fittings are not included. The connection kits shown at page must be purchased separately once. MonoCap TM HILIC-UP High Resolution 000 I.D. Length Cat.No A column stand is included. End-fittings are not included. The connection kits shown at page must be purchased separately once Flow Rate : 0.5 μl/min Injection Vol : 5 μl Detection : MS (TIC m/z ) Sample : Tryptic digest of proteins MonoCap HILIC-UP is an important addition to the MonoCap C8 High Resolution 000 column series. MonoCap HILIC-UP can retain highly hydrophilic peptides/proteins which may lead to discovering new peptides/proteins where a C8 phase couldn t identify. In HILIC, the higher the organic concentration, the greater the retention of more polar analytes. One of the biggest benefit of HILIC mode is, a high organic solvent concentration of the mobile phase will lead to a high sensitivity LC-MS/MS analysis. Reference: Hydrophilic Interaction Chromatography Using a Meter-Scale Monolithic silica capillary Column for Proteomics LC-MS, K Horie et al. Anal. Chem. 0, 86, 87-8 Application Specific Guard Preparative Capillary Applications Cat. No. Index 9

150 Monolithic Capillary HPLC MonoCap TM C8 Fast-flow Figure : Workable at High Flow Rates without Sacrificing Efficiency Flow Rate : μl/min Pressure :.9 MPa Column Eluent Col. Temp. Detection Injection Vol. Sample : MonoCap C8 for Fast-flow (50 0. mm I.D.) : A) CHCN B) HO A/B = 50/50, v/v : Ambient : UV 0 nm (MU70, Cell Volume 8 nl) : 0.5 μl :. Thiourea. Acetophenone. Benzene. Toluene 5. Naphthalene Workable at a broad range of linear velocity from 0.5 to 5 mm/s without sacrificing efficiency and separation at high speed. The number of theoretical plates produced by MonoCap C8 Fastflow is nearly equivalent to a totally porous particle type capillary column packed with a 5 μm packing material. are protected by either metal or PEEK hardwares. Five-fold Increase in Flow Rate Flow Rate Pressure : 0 μl/min : 9. MPa PEEK Metal I.D. Length Material of Hardware Cat.No. Cat.No. Cat.No Metal PEEK* Metal PEEK* Metal PEEK* Metal PEEK* For end-fittings information, please refer to page 8. For maximum operating pressure information, please refer to page 8. All 50 mm length PEEK columns does not come with a hardware and will be supplied with pcs of columns only. MonoCap TM C8 Nano-flow MonoCap C8 Nano-flow produces higher number of theoretical plates compared to a totally porous particle type capillary column packed with a μm packing material. It can be operated at a wide range of flow rate with low back pressure and achieve very high sensitive results in Nano-LC-ESI/MS applictions. are protected by either metal or PEEK hardwares. I.D. Length Material of Hardware Cat.No. Cat.No Metal PEEK* Metal PEEK* Metal PEEK* Metal PEEK* For end-fittings information, please refer to page 8. For maximum operating pressure information, please refer to page 8. All 50 mm length PEEK columns does not come with a hardware and will be supplied with pcs of columns only. 0

151 Monolithic Capillary HPLC MonoCap TM C8 WideBore MonoCap TM C8 Trap Column The MonoCap C8 Fast-flow is also available in 0.5 mm I.D. size, which can be used at a wide range of flow rate from 6 to 00 μl/min without sacrificing efficiency. The number of theoretical plates produced by MonoCap C8 WideBore is nearly equivalent to a totally porous particle type capillary column packed with a 5 μm packing material. are protected by metal hardwares. Length I.D. Material of Hardware Cat.No. Cat.No. Cat.No. 0.5 Metal For end-fittings information, please refer to page 8. For maximum operating pressure information, please refer to page 8. Reversed Phase HILIC Normal Phase MonoCap C8 Trap Column with Hardware (Top Image: /6 End-fittings, Bottom Image: / End-fittings) MonoCap Trap columns have a relatively big throughpore and workable at a high flow rate such as 0 μl/min. This benefit makes MonoCap Trap columns to be appropriate for on-line preconcentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection. End-fittings are /6 (0- UNF). / end-fittings are also available upon request. SEC I.D MonoCap TM Amide Amide groups are chemically bonded to the monolithic silica and makes it suitable for the analysis of sugars via HILIC mode. As the back pressure is significantly low, a 500 mm length MonoCap Amide column deliver over 0,000 plates offering high efficiency. Generally, HILIC mode uses acetonitrile at a concentration between % in an aqueous buffer such as ammonium acetate or ammonium formate, which have high solubility in organic solvents. are protected by either metal or PEEK hardwares. Length I.D. Hardware Cat.No. Cat.No. Cat.No. Metal PEEK Metal PEEK Metal PEEK For end-fittings information, please refer to page 8. For maximum operating pressure information, please refer to page 8. MonoCap TM SCX Length Hardware Cat.No. Cat.No. Cat.No. With Hardware Without Hardware With Hardware Without Hardware With Hardware Without Hardware MonoCap SCX is bonded with benzene sulfonic acid groups (strong cation exchange) and appropriate for D LC applications for the separation of biomolecules such as peptides and proteins. Length I.D. Material of Hardware Cat.No. Cat.No. Cat.No. Cat.No. Metal PEEK For end-fittings information, please refer to page 8. For maximum operating pressure information, please refer to page 8. Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index

152 Consumables and Accessories for Monolithic Capillary HPLC Connection Kit for MonoCap TM C8 High Resolution /6 SUS Nut / SUS Nut Connection Kit for MonoCap TM C8 High Resolution 000 A dedicated connection kit for MonoCap C8 High Resolution 000. Use this connection kit when connecting the column directly to the system. Description Contents of Kit Cat.No. /6 PEEK Ferrule /6 PEEK Sleeve / PEEK Ferrule / PEEK Sleeve Connection Kit for MonoCap C8 High Resolution 000 /6 PEEK Ferrule, SUS Nut, Sleeve pcs each / PEEK Ferrule, SUS Nut, Sleeve pcs each Zero Dead Volume Union Connect the tubing from the system to this union and install the column to achieve zero dead volume. Description P/N Orifice Remarks Cat.No. Zero Dead Volume Union U mm /6 Tubing SUS Male Nut, U- 78 μm Ferrule pcs each Connection Kit for MonoCap TM C8 Trap Column MonoCap C8 Trap Column Connection Kit /6 Description MonoCap C8 Trap Column Connection Kit /6 (Union Sleeve Capillary Tubing pcs each Nut Ferrule pcs each) MonoCap C8 Trap Column Connection Kit / (Union Sleeve Capillary Tubing pcs each Nut Ferrule pcs each) MonoCap C8 Trap Column Assembly Parts /6 (Nut Ferrule pcs each) MonoCap C8 Trap Column Assembly Parts / (Nut Ferrule pcs each) Cat.No

153 MonoSpray TM MonoSpray TM MonoSpray is an electrospray emitter for ESI-LC/MS which a monolithic packing is packed into a fused silica sprayer offering numbers of benefits compared to those traditional sprayers packed with particle based packings. Reversed Phase I.D. 50, 75, 00 μm Magnified View of MonoSpray Tip I.D.0 μm Benefits High Sensitivity Analysis The introduction of a monolithic packing into the sprayer deliver minimized sample diffusion resulting in high sensitivity. High Chemical Stability HILIC O.D. 70 μm I.D. 00 μm Length 50 mm Structure Cross Section View of Fused Silica Capillary Monolithic Silica The optimized surface treatment to the silica monolith eliminates non-specific adsorption issues. High Physical Stability Frits are not installed in MonoSpray to keep the monolithic packing in place, which results in offering simply longer lifetime and avoiding bed splitting problems compared to those traditional sprayers packed with particle based packings. Wide Range of Operational Flow Rates The very high porosity of monolithic packing allows a wide range of operational flow rates, even at high flow rates. Normal Phase SEC Cross Section View Example of Use Eluent MonoSpray MS MonoSpray Ion Exchange Connection to ESI-LC/MS 0.5 μl/min 0. μl/min Application Specific Ordering Information MonoSpray TM FS For online Nano-ESI-LC/MS. I.D. : 50, 75, 00 μm MonoSpray TM C8 Nano sprayer packed with octadecylated silica monolith offering reversed phased separation. I.D. : 75,00 μm MonoSpray TM FS Description Length O.D. (μm) Mono Spray FS Please inquire for other sizes. I.D. (μm) Qty Cat.No. (pcs) Flow Rate(μL/min) Change in Spray Shape at Different Flow Rates MonoSpray TM C8 Description Mono Spray C8 Nano FS 50 μm FS 00 μm FS 75 μm MonoSpray FS 0 Optimal Flow Rate against Each Column I.D. Size Length Please inquire for other sizes. O.D. (μm) C8 00 μm C8 75 μm MonoSpray C8 I.D. (μm) Qty Cat.No. (pcs) Guard Preparative Capillary Applications Cat. No. Index Based on monolithic technology, Merck KGaA, Darmstadt, Germany.

154

155 Applications Visit our website 6 Pharmacopeia 7 Pharmaceuticals 8 Biochemicals 9 Foods 0 Environmental Pesticides Vitamins Others 5 5

156 Applications Visit our website Provided a number of applications in various fields on our website. Please visit our website. Top of our website InertSearch Click here Various HPLC applications are available. The latest application is uploaded. LC Technical Note More detailed applications are available. 6

157 Applications Pharmacopeia Para-Hydroxybenzonate (JP) Crospovidone (JP) Reversed Phase HILIC Column : Inertsil ODS-(5 μm, 50.6 mm I.D.) Eluent : A) CHOH B) 50 mm KHPO A/B = /7, v/v Flow Rate :. ml/min Col. Temp. : 5 C Detection : UV 7 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LB097, LB098 Sample :. p-hydroxybenzoic acid. p-hydroxybenzoic acid methyl ester. p-hydroxybenzoic acid ethyl ester. p-hydroxybenzoic acid n-propyl ester Column : InertSustain C8 (5 μm, 50.0 mm I.D.) Guard Column : InertSustain C8 (5 μm, 5.0 mm I.D.) Eluent : A) CHCN B) HO A/B = /9, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Sample : Injection Vol. : 50 μl. -Vinyl--pyrrolidone Data Source : LC InertSearch No. LB67. Vinyl acetate Normal Phase SEC Losartan potassium and Hydrochlorothiazide (USP) Carumonam Sodium (JP) Ion Exchange Column : Inertsil C8- (0 μm, 50.6 mm I.D.) Eluent : A) CHCN B) 0 mm KHPO in HO (ph.5, HPO) A/B = 0/60, v/v Flow Rate :. ml/min Col. Temp. : 5 C Detection : UV 0 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LB00 Glycyrrhetinic acid in Glycyrrhiza (JP) Column : InertSustain C8(5 μm, 50.6 mm I.D.) Eluent : A) CHCN B). % CHCOOH in HO A/B = 0/60, v/v Flow Rate : 0.5 ml/min Col. Temp. : 0 C Detection : UV 5 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LB8 Sample :. Hydrochlorothiazide ( mg/l). Losartan potassium (55 mg/l) Sample :. Glycyrrhizic acid Column : Inertsil ODS- (5 μm, 50.0 mm I.D.) Eluent : A) 0. g/l (NH)SO B ) CHOH C) CHCOOH A/B = 97//, v/v/v Flow Rate :. ml/min Col. Temp. : 5 C Detection : UV 5 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LA99 Terbinafine Hydrochloride Cream (JP) Sample :. Resorcinol (.8 mg/l). Carumonam sodium (0. mg/l) Column : InertSustainSwift C8 (5 μm, 5.0 mm I.D.) Eluent : A) CHCN B) 0.5 % Tetramethylammonium hydroxide in HO (ph 8.0, % HPO in HO) C) THF A/B/C = //, v/v/v Flow Rate :.6 ml/min Col. Temp. : 5 C Sample : Detection : UV 8 nm. p -Terphenyl (7.5 mg/l) Injection Vol. : 0 μl. Terbinafine hydrochloride (00 mg/l) Application Specific Guard Preparative Capillary Applications Cat. No. Index 7

158 Applications Pharmaceuticals 7 Anti-Depressant Drugs 8 Drugs Time (min) Column : InertSustain C8 HP ( μm,50. mm I.D.) Eluent : A) 0. % HCOOH in CHCN B) 0. % HCOOH in HO A/B = / min - 0/ min - 0/60, v/v Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : LC/MS/MS (000 QTRAP : ESI, Positive, MRM) Injection Vol. : 5 μl Data Source : LC InertSearch No. LA908 Sample :. Sulpiride 0. Imipramine. Milnacipran. Nortriptyline. Trazodone. Maprotiline. Mianserin. Amitriptyline 5. Amoxapine. Trimipramine 6. Doxepin 5. Fluoxetine 7. Paroxetine 6. Sertraline 8. Desipramine 7. Clomipramine 9. Fluvoxamine (00 ng/ml each) Time (min) Column : Inertsil ODS- ( μm, 50. mm I.D) Eluent : A) CHCN B) 0.05 % HCOOH in HO A/B = 5/95-5 min - 95/5, v/v Flow Rate : 500 μl/min Col. Temp. : 0 C Detection : LC/MS/MS (000 QTRAP : ESI, Positive, MRM) Injection Vol. : 0 μl Sample :. Ranitidine 0. Diphenhydramine. Scopolamine. Doxepin. Naltrexone. Amitriptyline. Acetaminophen. Reserpine 5. Theophyline. Isopropylantipyrine 6. Metoprolol 5. Ketoprofen 7. Caffeine 6. Warfarin 8. Chlorpheniramine 7. Capsaicin 9. Propranolol 8. Dihydrocapsaicin Anti-inflammatory Drug Histamine Antagonist Column : InertSustain Phenyl ( μm, 50. mm I.D.) Eluent : A) CHOH/HCOOH = 00/0.05, v/v B) HO/HCOOH = 00/0.05, v/v A/B = 0/60, v/v Flow Rate : 0.6 ml/min Col. Temp. : 0 C Detection : LC/MS/MS (000 QTRAP:ESI, Positive, MRM) Injection Vol. : 5 μl Data Source : LC InertSearch No. LB Sample :. Hydrocortisone. Prednisolone (0. mg/l each) Column Eluent : Inertsil ODS- ( μm, 50. mm I.D.) : A) CHOH Flow Rate Col. Temp. Detection B) mm CHCOONH A/B = 0/60-0 min - 95/5 - min - 95/5, v/v : 0. ml/min : 0 C : LC/MS/MS (000 QTRAP : ESI, Positive, MRM) Sample :. Chlorpheniramine. Cinnarizin. Clemastine Injection Vol. : 0 μl Data Source : LC InertSearch No. LA678. Difenidol 5. Diphenhydramine 6. Diphenylpyraline 7. Hydroxyzine 8. Promethazine 9. Triprolidine (0. mg/l each) Drugs Cold Medication Column Eluent Flow Rate Col. Temp. Detection Injection Vol. Data Source : Inertsil ODS- ( μm, 00. mm I.D.) : A) CHCN B) 0. % HCOOH in HO A/B = 0/00-6 min - 0/90 - min - 00/0, v/v : 0. ml/min : 0 C : LC/MS/MS (000 QTRAP : ESI, Positive, MRM) : 0 μl : LC InertSearch No. LA60 Sample :. Sulbutamol (7.5 μg/l). Ranitidine (5.0 μg/l). Acetaminophen (5.0 μg/l). Theophylline (0.0 μg/l) 5. Metoprolol (.5 μg/l) 6. Caffeine (5.0 μg/l) 7. Propranolol (.5 μg/l) 8. Doxepin (5.0 μg/l) 9. Carbamazepine (5.0 μg/l) 0. Ketoprofen (5.0 μg/l). Indomethacin (0.0 μg/l) Column : Inertsil ODS- (5 μm, 50.6 mm I.D.) Eluent : A) CHCN B) 0. % HPO in HO A/B = /97-0 min - 75/5, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 0 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LA66 Sample :. Maleic acid. Acetaminophen. Caffeine. Chlorpheniramine 5. Ethenzamide 6. Bromovalerylurea 7. Apronalide 8. Isopropylantipyrine 9. Ibuprofen (50 mg/l each) 8

159 Applications Biochemicals Trypsin Digest of the Protein Reversed Phase HILIC 0 50 Column : MonoCap C8 High Resolution 000 (000 mm 0. mm I.D.) Trap Column : MonoCap C8 Trap Column (50 mm mm I.D.) Eluetnt : A) 0. % HCOOH in CHCN, B) 0. % HCOOH in HO A/B=0/ min - 5/55, v/v Flow Rate : 0.5 μl/min Col. Temp. : ambient Detection : LC/MS/MS (JMS-T00-LC, JEOL, TIC, m/z ) Injection Vol. : 5 μl Sample : tryptic digest of proteins Normal Phase SEC Nucleoside and Nucleic acid base Nucleic acid base Ion Exchange Application Specific Column : InertSustain C8 (5 μm, 50.6 mm I.D.) Eluent : 0. M NHPO, 0. M NaClO (ph.0, HPO) Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 60 nm Injection Vol. : μl Data Source : LC InertSearch No. LA895 Protein Column : Inertsil WP00 C8 (5 μm, 50.0 mm I.D.) Eluent : A) 0. % TFA in CHCN B) 0. % TFA in HO A/B = 0/80-0 min - 70/0, v/v Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : UV 80 nm Injection Vol. : 0 μl Data Source : LC Technical Note No. 6 Sample :. Cytosine. Uracil. Guanine. Adenine 5. Cytidine Uridine 7. Thymine 8. Adenosine 9. Guanosine 0. Thymidine Sample :. Ribonuclease B. Cytochrome c. Lysozyme. BSA 5. Ovalbumin Column : Inertsil Amide (5 μm, 50.6 mm I.D.) Eluent : A) CHCN B) 0 mm HCOONH A/B = 90/0, v/v Flow Rate : 0. ml/min Col. Temp. : 5 C Detection : UV 5 nm Injection Vol. : μl Data Source : LC InertSearch No. LB00 Catecholamines in Urine Column : Inertsil ODS- (5 μm, 50.0 mm I.D.) Eluent : A) Acetate-citrate buffer * : B) CHCN A/B = 00/6, v/v Flow Rate : 0.5 ml/min Col. Temp : 5 C Detection : ECD 800 mv vs. Ag/AgCl Injection Vol. : 0 μl Data Source : LC Technical Note No. 9 Sample :. Toluene. Thymine. Uracil. Uridine 5. Adenosine 6. Cytosine 7. Cytidine 8. Guanosine (0 mg/l each) Sample :. Norepinephrine (NE). Epinephrine (E).,-dihydroxybenzylamine (DHBA, I.S.). Dopamine (DA) (00 ng/ml in 0. % Acetic acid solution each) * : Acetate-citrate buffer : Dissolve 0.8 g of anhydrous sodium acetate,.0 g of citric acid monohydrate and 0.50 g of sodium -octanesulfonate in 500mL of HO. Guard Preparative Capillary Applications Cat. No. Index 9

160 Applications Foods Organic acid Linear Fatty acids Column : Inertsil Ph- (5 μm, 50.6 mm I.D.) + Inertsil CX (5 μm, 50.6 mm I.D.) Eluent : mm HClO in HO Reaction Reagent : 0. mm BTB + 0 mm NaHPO in HO Flow Rate : 0.5 ml/min Col. Temp. : 5 C Detection : VIS 0 nm Injection Vol. : 0 μl Data Source : LC Technical Note No. Sample:. Phosphoric acid 6. Lactic acid. Tartaric acid 7. Acetic acid. Malic acid 8. Succinic acid. Formic acid 9. Pyroglutamic acid 5. Citric acid ( mg/ml each) Column : InertSustain C8 (5 μm, 50.6 mm I.D) Eluent : A) 0. % HPO in CHCN B) 0. % HPO in HO A/B = 0/90-5 min - 90/0-0 min - 90/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 0 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LA90 Sample :. Acetic acid (C). Propionic acid (C). Butyric acid (C). Valeric acid (C5) 5. Caproic acid (C6) 6. Enanthic acid (C7) 7. Caprylic acid (C8) 8. Pelargonic acid (C9) 9. Capric acid (C0) 0. Undecanoic acid (C). Lauric acid (C). Tridecanoic acid (C). Myristic acid (C). Pentadecanoic acid (C5) ( mg/ml each) Preservatives and Sweetener Phenolic Antioxidants Column : InertSustain Phenyl (5 μm, 50.6 mm I.D.) Eluent : A)CHCN B)0. % HCOOH in HO A/B = 5/85, v/v Flow Rate :.0 ml / min Col. Temp. : 5 C Detection : UV 0 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LB0 Sample :. Acesulfame potassium. Saccharin. Sorbic acid. Benzoic acid 5. Dehydroacetic acid (0 mg/l each) Column : Inertsil Ph- (5 μm, 50.6 mm I.D.) Sample : Eluent : A) CHOH B) CHCN C) 5 % Acetic acid A/B/C = 0/0/60-5 min - 50/50/0, v/v/v (Mixed by a gradient mixer). Propyl gallate (PG).,,5-Trihydroxybutyrophenone (THBP). tert-butylhydroquinone (TBHQ). Nordihydroguaiaretic acid (NDGA) 5. Butylated Hydroxyanisole (BHA) Flow Rate :.0 ml/min 6. Octyl gallate (OG) Col. Temp. : 0 C 7. -Hydroxymethyl-,6-di-tert-butylphenol (HMBP) Detection : UV 80 nm 8. Dodecyl gallate (DG) Injection : 0 μl 9. Butylated hydroxytoluene (BHT) Data Source : LC Technical Note No. 65 (0 mg/l each) Cyanoguanidine and Melamine in Food Oxalic acid in Food Column : Inertsil HILIC (5 μm, 50.0 mm I.D.) Eluent : A) CHCN B) 0 mm Ammonium acetate A/B = 90/0-0.5 min - 90/0-5.5 min - 50/50, v/v Flow Rate : 0.5 ml/min Col. Temp. : 0 C Detection : LC/MS/MS (000 QTRAP : ESI, Positive, MRM) Injection Vol. : 5.0 μl Data Source : LC Technical Note No. Sample :. Cyanoguanidine (0 μg/l). Melamine (0 μg/l) Column : Inertsil Amide (5 μm, 50.6 mm I.D.) Eluent : A) CHCN B) 0 mm NaHPO in HO (ph 6.8, HPO) A/B = 65/5, v/v Flow Rate :.0 ml/min Col. Temp. : 50 C Detection : UV 0 nm Injection : 5 μl Data Source : LC Technical Note No.09 Sample :. Oxalic acid (00 mg/l) 0

161 Applications Foods Canthaxanthin in Tea Isoflavones Reversed Phase Column Eluent Flow Rate Col. Temp. Detection Injection Vol. Data Source : Inertsil ODS-( μm, 50.6 mm I.D.) : A) CHOH B) 0 mm NaHPO in HO A/B = 0/90-0 min - 50/50, v/v :.0 ml/min : 0 C : UV 80 nm : 0 μl : LC InertSearch No. LA Sample :. Gallocatechin (GC). Epigallocatechin (EGC). Catechin (C). Caffeine 5. Epigallocatechin gallate (EGCg) 6. Epicatechin (EC) 7. Gallocatechin gallate (GCg) 8. Epicatechin gallate (ECg) 9. Catechin gallate (Cg) Column : Inertsil ODS-SP (5 μm, 50.6 mm I.D.) Eluent : A) 0. % CHCOOH in CHCN B) 0. % CHCOOH in HO A/B = 5/85-8 min - 5/85 - min - 5/65, v/v Flow Rate :.5 ml/min Col. Temp. : 5 C Detection : PDA 5 nm Injection : 0 μl Data Source : LC Technical Note No Sample :. Daidzin (D). Glycitin (Gl). Genistin (G). 6 -O-Malonyldaidzin (MD) O- Malonylglycitin (MGl) O- Acetyldaidzin (AD) O- Acetylglycitin (AGl) O- Malonylgenistin (MG) 9. Daizein (De) 0. Glycitein (Gle). 6 -O- Acetylgenistin (AG). Genistein (Ge) (0 mg/l each) HILIC Normal Phase SEC Carotene in Food Tetracycline Ion Exchange Detection : VIS 55 nm Injection Vol. : 0 μl Data Source : LC Technical Note No.8 Column : Inertsil ODS-P (5 μm, 50.6 mm I.D.) Eluent : A) CHOH B) Ethanol A/B = 90/0, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Sample :. α-carotene. β-carotene Putrefactive Non-Volatile Amines in Food by Pre-column HPLC Column : Inertsil ODS-SP (5 μm, 50.6 mm I.D.) Sample : Guard Column : Inertsil ODS-SP (5 μm, 0.0 mm I.D.). Putrescine (5 mg/l) Eluent : A) CHCN B) HO A/B= 65/5, v/v. Cadaverine (5 mg/l) Flow Rate :.0 ml/min. Histamine (00 mg/l) Col. Temp. : 0 C.,8-Diaminooctane (0 mg/l) Detection : FL Ex 5 nm Em 55 nm 5. Tyramine (5 mg/l) Injection : 0 μl 6. Spermidine (5 mg/l) Data Source : LC Technical Note No Column : InertSustain C8 (5 μm, 50.6 mm I.D.) Sample :. Oxytetracycline Eluent : A) Imidazole buffer* B) CHOH A/B = 80/0, v/v (Premix). Tetracycline. Chlortetracycline ( mg/l each) Flow Rate :.0 ml/min * Imidazole buffer : Col. Temp. : 0 C Dissolve g of imidazole, 0.7 g of disodium Detection : FL Ex 80 nm Em 50 nm ethylenediaminetetraacetate and 0.7 g of Injection Vol. : 0 μl magnesium acetate in 800 ml of HO. Adjust to ph 7. with acetic acid and dilute this solution to,000 ml with HO. Food Dyes Column : Inertsil ODS- (5 μm, 50.6 mm I.D.) Eluent : A) CHCN B) 0 mm NaHPO in HO A/B = 0/90-50 min - 5/65, v/v 6 5 Sample :. Tartrazine (Food Yellow No., 7.6 mg/l). Amaranth (Food Red No.,.8 mg/l). Ingigocarmine (Food Blue No., 7.6 mg/l). New coccine (Food Red No. 0,.8 mg/l) 5. Sunset Yellow FCF (Food Yellow No. 5, 5. mg/l) 6. Naphthol Yellow S (7.6 mg/l) 7. Uranine (.8 mg/l) 8. Allura red AC (5. mg/l) 9. Ponceau R (7.6 mg/l) 7 8 Flow Rate :.0 ml/min Col. Temp. : 0 ºC Detection : UV 70 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LA509, 9, 0 5, Ponceau SX (5. mg/l). Orange I (5. mg/l). Fast green FCF (Food Green No.,.0 mg/l). Brilliant blue FCF (Food Blue No.,.0 mg/l). Ponceau R (7.6 mg/l) 5. Erythrosine (Food Red No., 5. mg/l) 6. Azure Blue VX (Sulfan blue,.0 mg/l) 7. Orange II (7.6 mg/l) 8. Acid red (Food Red No. 06,.0 mg/l) Application Specific Guard Preparative Capillary Applications Cat. No. Index

162 Applications Environmental Non-ionic Surfactant Anion Surfactant (Toluene addition sample) Column : InertSustain C8 (5 μm, 50.6 mm I.D.) Eluent : A) CHOH B) 0 mm NaBO7 in HO A/B = 8/6, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 50 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LA97 Sample :. Heptaoxyethylene dodecyl ether [Deriv.](0.00 mg/l) Column : Inertsil ODS- (5 μm, 50.6 mm I.D.) Eluent : 0. M NaClO in CHCN/HO = 65/5, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : FL Ex nm Em 8 nm Injection Vol. : 0 μl Data Source : LC Technical Note No. 0 Sample :. Sodium Decylbenzenesulfonate(C0). Sodium Undecylbenzenesulfonate(C). Sodium Dodecylbenzenesulfonate(C). Toluene 5. Sodium Tridecylbenzenesulfonate(C) 6. Sodium Tetradecylbenzenesulfonate(C) ( mg/l each) Haloacetic acids Chlorophenol Column : InertSustain C8 ( μm, 50.6 mm I.D.) Sample :. Monochloroacetic acid (MCAA)( μg/l) Eluent : A) CHOH B) 0. % HCOOH in HO A/B = 5/95-8 min - 00/0 - min - 00/0, v/v. Dichloroacetic acid (DCAA)( μg/l). Trichloroacetic acid (TCAA)(0 μg/l) Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : LC/MS/MS (000 QTRAP : ESI, Negative, MRM) Injection Vol. : 00 μl Data Source : LC Technical Note No. 5 Column Eluent Flow Rate Col. Temp. Detection : InertSustain C8 HP ( μm, 00. mm I.D.) : A) CHOH B) CHOH/HO = 0/90, v/v A/B = 0/60-8 min - 90/0-0.5 min - 90/0-0. min - 0/60-5 min - 0/60, v/v : 0. ml/min : 0 C : LC/MS (000 QTRAP : APCI, Neg, SIM) Injection Vol. : 5 μl Sample :. Phenol. -Chlorophenol. -Chlorophenol.,6-Chlorophenol 5.,-Chlorophenol 6.,,6-Chlorophenol (0.8 μg/l in HO each) Kinds of Aldehydes Aromatic Hydrocarbons Column : InertSustain C8 HP ( μm, 50.0 mm I.D.) Eluent : A) CHCN B) HO C) THF A/B/C = 5/55/0, v/v/v Flow Rate :.5 ml/min Col. Temp. : 0 C Detection : UV 60 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LA96 0 Sample :. DNPH-Formaldehyde. DNPH-Acetaldehyde. DNPH-Acetone. DNPH-Acrolein 5. DNPH-Propionaldehyde 6. DNPH-Crotonaldehyde 7. DNPH-Methylethylketone 8. DNPH-Methacrolein 9. DNPH-n-Butyraldehyde 0. DNPH-Benzaldehyde. DNPH-n-Valeraldehyde. DNPH-m-Tolualdehyde. DNPH-Hexanal (50 μg/l each) Column Eluent Flow Rate Col. Temp. Detection Data Source : Inertsil ODS-P (5 μm, 50.6 mm I.D.) : A) CHOH B) CHOH/HO = 70/0, v/v A/B = 0/00-5 min - 0/00-0 min - 00/0, v/v :.0 ml/min : 0 C : UV 5 nm : InertSearch for LC No. LA Sample :. Naphthalene 9. Benzo-[a]-anthracene. Acenaphthylene 0. Chrysene. Acenaphthene. Benzo-[b]-fluoranthene. Fluorene. Benzo-[k]-fluoranthene 5. Phenanthrene. Benzo-[a]-pyrene 6. Anthracene. Dibenzo-[a]-pyrene 7. Fluorene 5. Benzo-[ghi]-pyrene 8. Pyrene 6. Indeno-[,,-cd]-pyrene (0 ng/ml in CHOH each)

163 Applications Pesticides 60 pesticides Provided by AB SCIEX Reversed Phase HILIC Column : InertSustain C8 ( μm, 00. mm I.D.) Eluent : A) mm CHCOONH B) CHOH A/B = 5/ min - 0/ min - 95/5-5 min - 95/5, v/v Flow Rate : 0. ml/min Col.Temp. : 0 C Detection : LC/MS/MS (000 QTRAP : ESI, MRM) Injection Vol. : 0 μl Data Source : LC Technical Note No. 9 Normal Phase SEC 8 Pesticides Provided by Doctor KADOKAMI Tap Water Pesticides Positive Ion Exchange Column : Inertsil ODS- HP ( μm, 50. mm I.D.) Eluent : A) 5 mm CHCOONH in CHOH B) 5 mm CHCOONH in HO A/B = 5/95-0 min - 95/5-0 min - 95/5, v/v Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : LC/MS/MS (000 QTRAP : ESI, Positive, MRM) Injection Vol. :.5 μl Data Source : LC InertSearch No. LA Column : InertSustain Phenyl ( μm, 50. mm I.D) Eluent : A) 0. % HCOOH in CHCN B) 0. % HCOOH in HO A/B = 0/60-0 min - 70/0-0.0 min - 0/60-5 min - 0/60, v/v Flow Rate : 0. ml/min Col. Temp. : 0 C Detection : LC/MS/MS (000 QTRAP : ESI, Positive, MRM) Injection Vol. : 5 μl Data Source : LC InertSearch No. LB077 Sample :. Paclobutrazole. Diniconazole. Propiconazole. Difenconazole ( μg/l each) Negative Column : InertSustain C8 ( μm, 75. mm I.D.) Eluent : A) 5 mm CHCOONH in HO B) 5 mm CHCOONH in CHOH A/B = 95/5 - min - 60/0-5 min - 5/75-5 min - 0/00-6 min - 0/00, v/v Flow Rate : 0.5 ml/min Col.Temp : 0 C Sample.Temp. : 5 C Detection : LC/MS/MS (000 QTRAP : ESI, MRM) Injection Vol. : 00 μl Data Source : LC Technical Note No.5 Pesticides Carbamate Insecticides Sample :. Oxamyl. Methomyl. Aldicarb. Bendiocarb Carbaryl 6. Ethiofencarb 7. Pirimicarb 8. Isoprocarb 9. Fenobucarb 0. Methiocarb (.0 mg/l each) Column : InertSustain C8 (5 μm, 50.6 mm I.D.) Eluent : A) CHOH B) HO A/B = 5/65 - min - 5/65-0. min - 5/7-8. min - 5/7-0. min - 70/0-9. min - 70/0-0. min - 5/ min - 5/65, v/v Reaction Reagent : OPA reagent Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : FL Ex 9 nm Em 55 nm(0-8.5 min), Ex nm Em 8 nm( min), Ex 9 nm Em 55 nm( min) Injection Vol. : 0 μl Data Source : LC InertSearch No. LA Application Specific Guard Preparative Capillary Applications Cat. No. Index

164 Applications Vitamins Vitamin A in Food Vitamin B Column : Inertsil ODS- (5 μm, 50.6 mm I.D.) Eluent : A) CHOH B) HO A/B = 95/5, v/v Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 5 nm Injection Vol. : 0 μl Data Source : LC Technical Note No. Sample :. Retinol (50 μg/l) 0 6 Column : Inertsil ODS- (5 μm, 50.6 mm I.D.) Eluent : A) CHOH B) 0.0 M NaHPO, 0.5 M NaClO in HO (ph.) A/B = /9, v/v Reaction Reagent : 0.05 w/v % K[Fe(CN)6] +5 w/v % NaOH, 0. ml/min Flow Rate : 0.8 ml/min Col. Temp. : 0 C Detection : FL Ex 75 nm Em 0 nm Injection Vol. : 0 μl Data Source : LC Technical Note No. 0 Sample :. Thiamine. Hydroxyethyl thiamine (HET) (0 μg/l each) Vitamin C in Food Vitamin D, D Column : Inertsil SIL-00A (5 μm, 50.6 mm I.D.) Eluent : A) CHCOOCH5 B) n-hexane C) CHCOOH A/B/C = 50/0/0, v/v/v Flow Rate :.5 ml/min Col. Temp. : 0 C Detection : VIS 95 nm Injection Vol. : 0 μl Data Source : LC Technical Note No. 9 Sample :. Ascrobic acid Column : Inertsil ODS-P (5 μm, 50.6 mm I.D.) Eluent : CHCN Flow Rate :.5 ml/min Col. Temp. : 0 C Detection : UV 65 nm Injection Vol. : 00 μl Data Source : LC Technical Note No. Sample :. Vitamin D (Ergocalciferol). Vitamin D (Cholecarciferol) (0. mg/l each) Vitamin E Vitamin K Column : Inertsil NH (5 μm, 50.6 mm I.D.) Eluent : A) -Propanol B) n-hexane A/B = /98, v/v Flow Rate :. ml/min Col. Temp. : 0 C Detection : FL Ex 98 nm Em 0 nm Injection Vol. : 0 μl Data Source : LC InertSearch No. LB99 Sample :. α-tocopherol. β-tocopherol. γ-tocopherol. δ-tocopherol (.0 mg/l each) Column : InertSustain C8 (5 μm, 50.0 mm I.D.) Eluent : CHCN Flow Rate :.0 ml/min Col. Temp. : 0 C Detection : UV 70 nm Injection Vol. : 5 μl Data Source : LC InertSearch No. LB00 Sample :. Vitamin K (MK-). Vitamin K. Vitamin K (MK-7) (5 mg/l each)

165 Applications Others Sugar Analysis Column : InertSustain NH (5 μm, 50.6 mm I.D.) Sample : Eluent : A) CHCN B) HO A/B = 85/5, v/v. Rhamnose. Fucose. Fructose Flow Rate :.0 ml/min. Glucose Col. Temp. : 0 C 5. Sucrose Detection : RI 6. Maltose Injection Vol. : 0 μl Data Source : LC InertSearch No. LB80 7. Lactose (0 mg/ml each) 6 7 Sugar Analysis Using HPLC-ECD Column : InertSphere Sugar- (5 μm, 50.6 mm I.D.) Eluent : 00 mm NaOH Flow Rate : 0.5 ml/min Col. Temp. : 5 C Detection : ECD Pulse Mode Injection Vol. : 0 μl Data Source : LC Technical Note No. 0 5 Sample :. Fucose. Glucose. Fructose. Lactose 5. Sucrose (0 mg/l each) Reversed Phase HILIC Normal Phase SEC Analysis of Pre-column Derivatized Amino Acids Column : Inertsil ODS-( μm, 50.0 mm I.D.) Eluent : A) CHCN/CHOH/HO = 5/0/5, v/v/v B) 0 mm KHPO (ph 6.9, HPO) A/B = /89 - min - /89-9 min - /78 - min - 8/7-9 min - 0/70 - min - 65/5-7 min - 75/5 - min - 00/0, v/v Flow Rate : 0.7 ml/min Col. Temp. : 5 C Detection : FL Ex 50 nm Em 50 nm (0-9 min) Ex 66 nm Em 05 nm (9-5 min) Injection Vol. : μl Sample : Derivatized Amino Acids Data Source : LC InertSearch No. LB Analysis of Pre-column Derivatized Aflatoxin, Sample :. OPA-Aspartic Acid. OPA-Tyrosine. OPA-Glutamic Acid. OPA-Cys-Cys. OPA-Asparagine 5. OPA-Valine. OPA-Serine 6. OPA-Methionine 5. OPA-Glutamine 7. OPA-Tryptophan 6. OPA-Histidine 8. OPA-Phenylalanine 7. OPA-Glycine 9. OPA-Isoleucine 8. OPA-Threonine 0. OPA-Leucine 9. OPA-Citrulline. OPA-Lysine 0. OPA-Arginine. Fmoc-Proline. OPA-Alanine (0 μg/ml each). OPA-GABA (-aminobutanoic acid) Column : InertSustain C8 (5 μm, 50.6 mm I.D.) Sample : Eluent : A) CHOH B) CHCN C) HO A/B/C = 0/0/60, v/v/v (Premix). Aflatoxin G. Aflatoxin B Flow Rate :.0 ml/min. Aflatoxin G Col. Temp. : 0 C. Aflatoxin B Detection : FL Ex 65 nm Em 50 nm (5 ng/ml each) Injection Vol. : 0 μl Data Source : LC InertSearch No. LB07 PFOS and PFOA Column : Inertsil ODS- ( μm, 00. mm I.D.) Sample :. PFOA (Perfluorooctanoic acid) Eluent : A) 5 mm CHCOONH in HO B) 5 mm CHCOONH in CHOH A/B = 0/60-8 min - 5/75-0. min -0/ min - 0/90-0. min - 0/ min - 0/90, v/v. PFOS (Perfluorooctanesulfonic acid) ( mg/l each) Flow Rate : 0.6 ml/min Col. Temp. : 0 C Detection : LC/MS/MS (000 QTRAP : ESI, Negative, MRM) Injection Vol. : μl Data Source : LC InertSearch No. LA86 Hydrogen Peroxide (HO) Column : Inertsil CX (5 μm, 50.6 mm I.D.) Flow Rate : 0.8 ml/min Detection : ECD Injection Vol. : 00 μl Data Source : LC Technical Note No. 9 Sample :. Hydrogen peroxide(ho) (0 μg/l) Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 5

166 Cat.No. INDEX / / / /

167 Cat.No. INDEX Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 7

168 Cat.No. INDEX / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / /

169 Cat.No. INDEX /7/9//5/ 7/9////5/7/ 9/0///5/7/9/ ///5/9/5/5/55/ 59/6/6/65/67/67/69/7 /75/79/8/8/85/ /7/9//5/ 7/9////5/7/ 9/0///5/7/9/ ///5/9/5/5/55/ 59/6/6/65/67/67/69/7 /75/79/8/8/85/ Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 9

170 Cat.No. INDEX / /

171 Cat.No. INDEX Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 5

172 Cat.No. INDEX

173 Cat.No. INDEX / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 5

174 Cat.No. INDEX / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / /

175 Cat.No. INDEX Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 55

176 Cat.No. INDEX / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / /7 56

177 Cat.No. INDEX / / / / / / / / / / / / / / / / / / / / / / / Reversed Phase HILIC Normal Phase SEC Ion Exchange Application Specific Guard Preparative Capillary Applications Cat. No. Index 57

178 Trademarks The following trademarks are the property of the listed company and/or its affiliates. GL Siences Inc. in Japan Inertsil InertSustain InertSphere MonoCap MonoSpray SILFILTER AB SCIEX QTRAP Waters Technologies Corporation UPLC 58

179 InertSustain, Inertsil, Sales Network ARGENTINA DENMARK 7 JORDAN 0 SOUTH AFRICA AUSTRALIA 5 EGYPT 8 KOREA SPAIN AUSTRIA 6 FINLAND 9 MALAYSIA SRI LANKA AZERBAIJAN 7 FRANCE 0 MALTA SUDAN 5 BELGIUM 8 GERMANY MOROCCO SWEDEN 6 BRAZIL 9 HUNGARY The NETHERLANDS 5 SWITZERLAND 7 BULGARIA 0 ICELAND NORWAY 6 TAIWAN 8 CANADA INDIA PAKISTAN 7 THAILAND 9 CHILE IRAQ 5 PHILIPPINES 8 TURKEY 0 CHINA INDONESIA 6 POLAND 9 UNITED KINGDOM COLOMBIA IRELAND 7 SAUDI-ARABIA 50 UNITED STATES CROATIA 5 ISRAEL 8 SINGAPORE 5 VIETNAM CZECH REPUBLIC 6 ITALY 9 SLOVAKIA 59

180 Worldwide Ordering Information GL Sciences Inc. Japan GL Sciences B.V. GL Sciences Inc. USA Distributor: - Nishishinjuku 6-Chome De Sleutel 9, 565 AS Eindhoven 7 Torrance Blvd. Suite 55 Shinjuku-ku, Tokyo, 6-0, Japan The Netherlands Torrance, CA 9050 Phone: Phone: + (0)0-595 Phone: Fax: Orders: orders@glsciences.eu Fax: Web: Web: Web: world@gls.co.jp info@glsciences.eu info@glsciencesinc.com