ACE Excel SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability

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1 ACE Excel SuperC8 TM Ultra-Inert UHPLC and HPLC s with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS-compatible buffers between ph.. High efficiency µm, µm, µm and 0µm particles for UHPLC and HPLC Ultra-inert for maximum performance and reproducibility ACE UHPLC and HPLC s TM

2 ACE Excel SuperC8 Ultra-Inert UHPLC and HPLC s with Extended ph Stability ACE Excel SuperC8 Ultra-Inert UHPLC and HPLC s with Extended ph Stability Ideal column choice for method development Exploit selectivity changes at low, intermediate and high ph Excellent column lifetime reduces the cost per analysis Specially designed for high and low ph mobile phases Designed for use with LC/MS-compatible buffers Recommended ph range.. No hysteresis problems when switching phs Ultra-Low Bleed for Improved LC/MS Compatibility Compatible with both MeOH and ACN Rapid column equilibration without memory effects Preparative dimensions available Reproducible scale up from analytical separations up to and 0µm particle sizes Exploit increased loading capacity of basic analytes at elevated ph Exploit Selectivity by Adjusting ph Application # 0 If ACE Excel SuperC8 does not outperform the column you are currently using, simply contact us for a full refund and keep the ACE Excel SuperC8 column (.mm -.6mm id) FREE OF CHARGE. ph 6 ph ph ph 7 0 ph ph ph 6 ph ph 7 ph 8 0 ph 9 ph 0 6 : ACE Excel µm SuperC8, 0 x.mm Sample: ) nizatidine ) salbutamol ) amiloride ) N-acetylprocainamide ) quinoxaline 6) methyl paraben 7) p-cresol 8) reserpine 9) piperine 0) toluene ) felodipine Temperature: 0 C Flow Rate: 0.ml/min Wavelength: nm Gradient: 00% B in 7 minutes Acidic Mobile Phase: A: 0mM ammonium formate in H O (ph.0) B: 0mM ammonium formate (ph.0) in 90:0 (v/v) MeCN/H O Basic Mobile Phase: A: 0.% NH (= 8mM) in H O (ph 0.7) B: 0.% NH (=8mM), ph 0.7 in 90:0 (v/v) MeCN/H O ph ph

3 ACE Excel SuperC8 Ultra-Inert UHPLC and HPLC s with Extended ph Stability Encapsulated Bonding Technology (EBT ) for Improved Chromatography and Stability Traditional C8 Bonding ACE Excel SuperC8 with Encapsulated Bonding Technology (EBT ) C8 Endcapping group Unbonded silanol group C8 The unique Encapsulated Bonding Technology (EBT ) developed for ACE Excel SuperC8 columns dramatically increases ligand coverage of the silica surface and effectively eliminates the negative effect of unbonded silanol groups from separations. This higher ligand coverage results in improved inertness, chromatographic performance and stability. Many C8 bonded columns will exhibit ligand cleavage under acidic conditions, resulting in retention shifts and/or increased peak tailing. Under aggressive acidic conditions (see Figure ) ACE Excel SuperC8 is highly resistant to ligand cleavage, due to a combination of the Encapsulated Bonding Technology (EBT ) and ultra-inert ACE silica. Under basic conditions with LC/MS-compatible mobile phases (see Figure ), the Encapsulated Bonding Technology (EBT ) shields the ACE Excel SuperC8 surface from dissolution while maintaining excellent chromatographic performance. Traditional C8 bonded silica columns are prone to silica dissolution under these aggressive conditions, which can result in premature column deterioration. Figure - Excellent Acidic Stability at ph.8 Application # Acidic flow conditions : ACE Excel µm SuperC8, 0 x.mm Mobile Phase: 0:0 MeOH/0.% TFA in H O (ph.8) Temperature: 0 C Flow Rate: 0.0ml/min Figure - Excellent Basic Stability at ph 0.7 Application # Basic flow conditions : ACE Excel µm SuperC8, 0 x.6mm Mobile Phase: 0:0 ACN/0.% NH in H O (ph 0.7) Temperature: 0 C Flow Rate:.00ml/min Day k (phenanthrene) = 6.9 Day N0. (biphenyl) = 69,00pl/m Acidic Mobile Phase (ph.8) (continuous flow) >0,000 column volumes >,000 injections Basic Mobile Phase (ph 0.7) (continuous flow) >0,000 column volumes >,000 injections Day 0 k (phenanthrene) = 6.8 Day 0 N0. (biphenyl) = 68,700pl/m Evaluation conditions Sample: ) uracil ) dimethyl phthalate ) toluene ) biphenyl ) phenanthrene Mobile Phase: 70:0 MeOH:H O Temperature: C Flow Rate: 0.0ml/min Wavelength: nm Evaluation conditions Sample: ) uracil ) dimethyl phthalate ) toluene ) biphenyl ) phenanthrene Mobile Phase: 80:0 MeOH:H O Temperature: C Flow Rate:.00ml/min Wavelength: nm

4 ACE Excel SuperC8 Ultra-Inert UHPLC and HPLC s with Extended ph Stability Comparison of Inertness at Intermediate ph Leading column brands in 0 x.mm LC/MS-compatible dimensions at ph.8 Silica, Hybrid and Superficially Porous particle technologies were compared Comparison of column efficiency for pyridine, a basic molecule Efficiency measured at % peak height to account for peak tailing effects Peak Efficiency Comparison Reproduced with kind permission of The Open University, UK. Application # ACE Excel SuperC8 Zorbax Eclipse.8 XDB C8,00pl/m 68,900pl/m Lower Tailing Agilent Poroshell.7 0 EC C8,900pl/m Waters XBridge. BEH C8 XP 0,800pl/m Waters Acquity.7 BEH C8 0,700pl/m Phenomenex Kinetex.6 C8 6,800pl/m Waters XBridge. C8,600pl/m Phenomenex Luna. C8() HST a,900pl/m Nucleoshell.7 RP8 a,00pl/m Phenomenex Kinetex.7 C8 0,700pl/m Zorbax Extend.8 C8 Thermo Accucore.6 C8 0,00pl/m 7,00pl/m Higher Tailing 0 0,000 0,000 60,000 80,000 00,000 N0.0 (pyridine) (plates/m) ACE Excel SuperC8 (fully porous ultra-inert silica) N 0.0 (pyr) = 68,900pl/m Waters ACQUITY.7 BEH C8 (hybrid particle) N 0.0 (pyr) = 0,700pl/m Phenomenex Kinetex.7 C8 (core-shell particle) N 0.0 (pyr) = 0,700pl/m Dimensions: 0 x.mm ( a 0 x.0mm) Sample: ) uracil ) pyridine ) phenol Mobile Phase: 0:70 MeOH/0mM NH OAc in H O (ph.8) Flow Rate: 0.0ml/min Temperature: C Wavelength: nm Comparative data may not be representative of all applications. Please see page for acknowledgement of trademarks. Conclusion: Significant differences in efficiency, peak shape and selectivity are seen when analyzing pyridine a basic molecule. Increased tailing and retention, combined with reduced efficiency, are indicative of undesirable secondary interactions between pyridine and silanol groups on the stationary phase surface. These interactions result in poor column reproducibility. ACE C8 columns have gained a well deserved reputation for providing excellent efficiency, peak shape and reproducibility. ACE Excel SuperC8 columns further build upon this proven reputation to provide the analyst with even better chromatographic performance across an extended range of ph conditions under LC/MS-compatible mobile phase conditions. Si-OH ACE Stationary Phases Virtually Eliminate the Negative Effects of Silanols on UHPLC & HPLC Separations

5 ACE Excel SuperC8 Ultra-Inert UHPLC and HPLC s with Extended ph Stability Comparison of Inertness at High ph LC/MS-compatible mobile phase at ph 0.7 Leading column brands in 0 x.6mm dimensions Comparison of column efficiency for pyridine, a basic molecule Efficiency measured at % peak height to account for peak tailing effects Peak Efficiency Comparison Reproduced with kind permission of The Open University, UK. Application # ACE Excel SuperC8 Phenomenex Gemini C8 Waters XBridge. BEH C8 Phenomenex Gemini-NX C8 Waters XSelect. CSH C8 ZORBAX Extend. C8 6,600pl/m,00pl/m,000pl/m,000pl/m 0,000pl/m,00pl/m 0 0,000 0,000 60,000 80,000 00,000 0,000 0,000 N0.0 (pyridine) (plates/m) ACE Excel SuperC8 N 0.0 (pyr) =,00pl/m Phenomenex Gemini C8 N 0.0 (pyr) = 6,600pl/m Waters XBridge. BEH C8 N 0.0 (pyr) =,00pl/m Phenomenex Gemini-NX C8 N 0.0 (pyr) =,000pl/m Waters XSelect. CSH C8 N 0.0 (pyr) =,000pl/m ZORBAX Extend. C8 N 0.0 (pyr) = 0,000pl/m Dimensions: 0 x.6mm Sample: ) uracil ) pyridine ) phenol Mobile Phase: 0.% NH (=8mM), ph 0.7 in 60:0 (v/v) MeOH/H O Flow Rate:.00ml/min Temperature: C Wavelength: nm Comparative data may not be representative of all applications. Please see page for acknowledgment of trademarks. Conclusion: When analyzing a basic molecule (pyridine) at high ph, differences in efficiency, peak tailing and retention are seen. This suggests undesirable secondary interactions between the basic molecule and silanol groups on the stationary phase surface still remain. Further to the intermediate ph comparison (see page ), the ACE Excel SuperC8 again provides excellent peak shape and efficiency when analyzing a basic molecule (pyridine) at high ph, which indicates undesirable secondary interactions have been virtually eliminated. These undesirable interactions can also result in poor column reproducibility. Changing ph is a powerful tool to enable the selectivity to be optimized and sample impurities to be identified. ACE Excel SuperC8 columns are designed to provide excellent peak shape and lifetime under acidic, intermediate and high ph conditions with LC/MS-compatible buffers, and may additionally be used with mobile phases containing MeOH or ACN without suffering efficiency loss and/or extended equilibration times. Si-OH ACE Stationary Phases Virtually Eliminate the Negative Effects of Silanols on UHPLC & HPLC Separations

6 ACE Excel SuperC8 Ultra-Inert UHPLC and HPLC s with Extended ph Stability Product Availability and Specifications Phase Functional Group Endcapped Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) Maximum ph Range ACE Excel SuperC8 Octadecyl encapsulated Yes,,, a L USP Listing a ACE Excel SuperC8 is designed for use with LC/MS-compatible buffers. Further information is contained within ACE SuperC8 A Guide to Buffer Selection - please contact your distributor to request your FREE copy or visit To further extend UHPLC and HPLC column lifetimes, ACE pre-column filters are recommended for further details please contact MAC-MOD Analytical or visit For HPLC column connections up to 6000psi, PEEK fingertight fittings (p/n ACE-CC0) are recommended for further details please contact MAC-MOD Analytical or visit For UHPLC column connections up to 000psi, reuseable fittings (p/n EXL-CC0) are recommended for further details please contact MAC-MOD Analytical or visit ACE Excel µm SuperC8 UHPLC/HPLC s (supplied in dual compatible UHPLC/HPLC Excel hardware format with 000bar/000psi pressure limit) 0mm 0mm mm 0mm 7mm 00mm mm 0mm.mm EXL-0-00U EXL-0-00U EXL-0-0U EXL-0-00U EXL-0-70U EXL-0-00U EXL-0-0U EXL-0-0U.0mm EXL-0-00U EXL-0-00U EXL-0-0U EXL-0-00U EXL-0-70U EXL-0-00U EXL-0-0U EXL-0-0U.6mm EXL-0-06U EXL-0-06U EXL-0-6U EXL-0-06U EXL-0-76U EXL-0-06U EXL-0-6U EXL-0-6U PRICE $ $ $ $90 $66 $69 $70 $780 ACE Excel µm SuperC8 UHPLC/HPLC s (supplied in dual compatible UHPLC/HPLC Excel hardware format with 000bar/000psi pressure limit) 0mm 0mm mm 0mm 7mm 00mm mm 0mm 0mm.mm EXL--00U EXL--00U EXL--0U EXL--00U EXL--70U EXL--00U EXL--0U EXL--0U EXL--0U.0mm EXL--00U EXL--00U EXL--0U EXL--00U EXL--70U EXL--00U EXL--0U EXL--0U EXL--0U.6mm EXL--06U EXL--06U EXL--6U EXL--06U EXL--76U EXL--06U EXL--6U EXL--6U EXL--6U PRICE $0 $80 $9 $0 $70 $6 $67 $70 $80 ACE Excel µm SuperC8 UHPLC/HPLC s (supplied in dual compatible UHPLC/HPLC Excel hardware format with 000bar/000psi pressure limit) 0mm 0mm mm 0mm 7mm 00mm mm 0mm 0mm.mm EXL--00U EXL--00U EXL--0U EXL--00U EXL--70U EXL--00U EXL--0U EXL--0U EXL--0U.0mm EXL--00U EXL--00U EXL--0U EXL--00U EXL--70U EXL--00U EXL--0U EXL--0U EXL--0U.6mm EXL--06U EXL--06U EXL--6U EXL--06U EXL--76U EXL--06U EXL--6U EXL--6U EXL--6U PRICE $0 $60 $70 $90 $7 $60 $6 $690 $70 ACE µm SuperC8 Semi-Prep and Preparative HPLC s 0mm 7mm 00mm mm 0mm 0mm 7.7mm ACE--008 ACE--708 ACE--008 ACE--08 ACE--08 ACE mm ACE--00 ACE--70 ACE--00 ACE--0 ACE--0 ACE--0.mm ACE--00 ACE--70 ACE--00 ACE--0 ACE--0 ACE--0 Prices Available upon request. ACE 0µm SuperC8 Analytical, Semi-Prep and Preparative HPLC s (please enquire) UHPLC and HPLC s 0 Commons Court, Chadds Ford, PA 97 Phone: (800) -708 Fax: (60) 8-99 Website: ACE is a registered trademark of Advanced Chromatography Technologies Limited. ACE Excel SuperC8, ACE Excel and EBT are trademarks of Advanced Chromatography Technologies Limited. Advanced Chromatography Technologies Limited acknowledges the registered and unregistered trademarks of Agilent Technologies Inc., Macherey-Nagel GmbH & Co. KG, Phenomenex Inc., Thermo Fisher Scientific and Waters Corporation and has no affiliation with any of these companies. 000Q/0/00-A00

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