LC800. Smart HPLC. Until now UHPLC From now Smart HPLC

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1 LC800 Smart HPLC Until now UHPLC From now Smart HPLC

2 Smart HPLC Leads to Ultimate Performance Patent Pending S

3 LC800 is a completely new and unique concept, designed for maximum performance in high resolution of separations. Take a look at the column oven below and you can see how we have combined all the thermally affected parts inside the column oven. Not only the fluid path is kept as short as possible to minimize dead-volume, but all the tubing connections remain inside the oven. Injection Valve HPLC Column Gradient Mixer Built-in UV detector flow cell unit (other detectors also can be used) The gradient mixer, injector and flow cell are all equilibrated at the setting temperature as the separation column; the result is a significant reduction in band-broadening and sharper peaks, which leads to a dramatic increase in sensitivity and separation. ART HPLC UHPLC and HPLC are now in one Smart HPLC.

4 Smart HPLC LC800 UHPLC Smart HPLC HPLC LC800 is a new and innovative development concept offering maximum performance for your analysis. Until now, compounds that were difficult to be separated, but these can now be separated easily with LC800. High separation using UHPLC columns are greatly influenced by the system dead-volume. To overcome this we have designed a fully integrated system, consisting of gradient pump, a high capacity autosampler and a range of detectors, together with the unique concept of all the critical system components being built into the column oven. The injection valve, gradient mixer and detector flow cell are thermally stabilized to prevent solvent temperature gradients that can reduce separation efficiency. Hideyuki Uzu LC800 Specialist, R & D Dept.

5 Minimized Dead - volume Leads to High Performance Separation Until now, compounds that were difficult to be separated, but these can now be separated easily with LC800. LC800 UHPLC 4 Column : Inertsil ODS-4 ( μm, 50. mm I.D) Eluent : CHCN / HO = 65 / 5, v / v Flow Rate : 0.6 ml / min Injection Vol : μl Col. Temp : 40 C Detection : UV 54 nm Time(min) Sample.Acetophenone.Benzene.Toluene Naphthalene Time(min) With long length and large volume of tubing, the peaks become broad. Our integrated system offers the minimized dead-volume. From UHPLC to Smart HPLC Smart HPLC demonstrates the true efficiency of your HPLC column. Using UHPLC components that previously were difficult to separate but these can now be separated easily by LC800. UHPLC requires the use of sub μm packing materials to achieve e high speed separation. Smart HPLC takes advantage of larger r particle size columns to achieve the same rapid separation. There are many benefits in using Smart HPLC, the system and column lifetime are greatly increased due to the lower working pressures and lower possibility of suffering from column-clogging. The improved system performance also means reduced downtime and lower operating costs. So why don't you try Smart HPLC, LC800? Analytical Column ODS.7 μm Inertsil ODS-4 μm Back Pressure High Low Theoretical plate number (Naphthalene) % UP! LC800 UHPLC 5 % UP! Low pressure, but High theoretical plate number! LC800 UHPLC 4

6 In pursuit of excellent performance Low Carry Over Autosampler LC800 s unique injector washing system means carry-over is virtually eliminated, even in high sensitivity analysis. 00 ng / ml Reserpine Blank Column : Inertsil ODS- ( μm,. 50 mm I.D.) Eluent : A) CHCN B) 0. % HCOOH in HO A / B = 60 / 40, v / v Flow Rate : 0.6 ml / min Col. Temp. : 40 C Detector : LC / MS / MS (ESI, Positive, MRM) 4000Q TRAP (AB SCIEX) Sample Tray LC800 has a high sample capacity, with up to 60 vials(.5 ml) and with a range of different tray options.(option: up to 6 microplates Maintenance Monitor The compact key pad is simple to use with powerful functionality and fits neatly inside the instrument when not being used. 5

7 Benefits of temperature control When performing very high speed analysis, it is possible that the retention time can become irreproducible due to fluctuations in mobile phase temperature. LC800 offers complete thermostatic stability by building the gradient mixer inside the column oven; the result is excellent retention time reproducibility. Flow Cell with Temperature Control AU C Flow Cell without Temperature Control AU C Cell Temp Cell Temp AU 0.00 Baseline min C AU C Baseline min C Eluent : A) 0.05 % TFA in CHOH B) HO A / B = 5 / 75 Flow Rate : 0. ml / min Detection : UV 4 nm GL Sciences Inc. is certified for ISO 400. GL Sciences Inc. recognizes that the global environmental protection is one of the most important issues to all humankind and that it is essential for companies to try to reduce the environmental burden in order to continue and grow. We contribute to realize a better society through the global environmental protection conscious cooperate activities. The Fukushima Factory is certified for ISO 900. Koji Suzuki Application Specialist GL Sciences Inc. Fukushima factory obtained an International Organization for Standardization ISO 900:008 for quality management system to design, produce and supply instruments, parts, peripheral devices, columns, bulk materials, reagents and spectroscopic cells for gas chromatography and liquid chromatography. 6

8 Powerful Support for LC/MS High separation efficiency, Minimized dead-volume With the benefits offered by using an integrated oven, it is now possible to separate compounds that previously could not be separated. LC800 system can be used with both a general purpose detector, such as UV-visible etc., also with LC/MS detectors where ion suppression can have a significant effect. Analysis with Smart HPLC offers the sharper peaks with increased peak height and leads to increased sensitivity. AB SCIEX LC800 UHPLC 5s 9s LC System : LC800 Column : Inertsil ODS- ( μm, 50. mm I.D.) Eluent : A) HO B) CHOH A / B = 50 / 50, v / v Flow Rate : 0.5 ml / min Injection Vol : 5 μl Col. Temp. : 40 C Detection : LC / MS / MS (ESI, Positive, MRM) 4000Q TRAP (AB SCIEX) min 5 Sample. Oxamyl. Methomyl. Aldicarb 4. Bendiocarb 5. Pirimicarb 6. Methiocarb min 5 7

9 Maximum Efficiency The minimized dead-volume increased column performance, and that achieved reducing analysis time. Not just for high speed analysis, but also for routine separations. To get the best out of your column, make sure you choose a system which doesn t compromise on performance. LC800 UHPLC Low theoretical plate number Time, min Time, min Column : Inertsil ODS-4 ( μm, 50. mm I.D.) Eluent : A) 5 mm Ammonium acetate in CHOH B) 0 mm Ammonium acetate in HO A / B = 85 / 5 - min - 60 / min 60 / min - 50 / 50 - min 45 / min - 5 / min - 5 / 95, v / v Flow Rate : 0. ml / min Col. Temp. : 40 C Detection : LC / MS / MS (ESI, Positive, MRM) 4000Q TRAP (AB SCIEX) Sample : Pesticides 8

10 The First Choice Column Inertsil ODS-4 Inertsil, the name of our brand comes from the key word INERT, which is the most important factor in the separation analysis. GL Sciences Inertsil columns delivers the maximum performance of an HPLC column and we would like to support you and your critical analysis. Inertsil ODS-4 column is the most recent addition to the Inertsil line and the most inert columns available.superior peak shapes for all various compounds along with extreme stability under 00 % aqueous mobile phases. Inertsil ODS-4 is just compatible with any type of compounds in any mobile phase conditions delivering highly stable chromatograms for qualitative and quantitative analysis. For more information, please visit our website at : Basic Compound Test Inertsil ODS-4 Atlantis T Zorbax Eclipse Plus C8 Luna 5μm C8() Column : ODS(5 μm, mm I.D.) Eluent : A) CHCN B) 5 mm Phosphate buffer ph7.0 A / B = 40 / 60, v / v Flow Rate :.0 ml / min Col. Temp. : 40 C Detection : UV 0 nm Injection Vol. : μl Sample :. Phenol (0.05 mg / ml). Dextromethorphan HBr (0. mg / ml) Acidic Compound Test Inertsil ODS-4 Atlantis T Zorbax Eclipse Plus C8 Luna 5μm C8() Column : ODS(5 μm, mm I.D.) Eluent : A) CHCN B) 0. %HPO4 A / B = 5 / 75, v / v Flow Rate :.0 ml / min Col. Temp. : 40 C Detection : UV 54 nm Injection Vol. : μl Sample :. Brilliant Blue FCF (0.05 mg / ml). Phenol (0. mg / ml). Salicylic acid (0. mg / ml) 9

11 HPLC and UHPLC are now in one Smart HPLC, LC800. Minimized dead-volume is designed since a column and a detector set in the oven. Dr.Xiaojing Zhou, LC800 Developer, Deputy General Manager of R & D Dept. Until now UHPLC From now Smart HPLC We, GL Sciences do support for your success. Tommy Yoshinaka International Sales Manager 0

12 For more information Visit our Web site: Contact your local GL Sciences Authorized Distributor We reserve the right to change specifications to make improvements without notice. Distributor: - Nishishinjuku 6-chome, Shinjuku-ku, Tokyo 6-0. Japan Tel Fax

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