InertSustain AQ-C18. HPLC, LC/MS Columns. Maximizing retention for highly polar compounds in reversed phase methods with highly aqueous mobile phases
|
|
- Ashlynn Wilkinson
- 5 years ago
- Views:
Transcription
1 HPLC, LC/MS Columns InertSustain AQ-C8 Maximizing retention for highly polar compounds in reversed phase methods with highly aqueous mobile phases Physical Properties Silica : ES (Evolved Surface) Silica Gel Particle Size :.9 μm, μm, μm Surface Area : 0 m /g Pore Size : 00 Å (0 nm) Pore Volume : 0.8 ml/g Bonded Phase : Octadecyl Groups End-capping : Complete Carbon Loading :.0 % USP Code : L ph Range : ~0 Authorised Distributor: Hichrom Limited sales@hichrom.co.uk
2 InertSustain AQ-C8 Benefits. Exceptional retention for highly polar compounds (hydrophilic). Highly inert packing material results in less tailing of peaks for virtually any type of analytes. Extreme resistance to low and high ph mobile phases. Endlessly reproducible from column-to-column and batch-to-batch InertSustain AQ-C8 columns are designed to achieve strong retention for highly polar compounds, which is the most challenging goals in developing reversed phase methods. The optimization of bonding of the C8 groups at equal distance to the silica gel enable InertSustain AQ-C8 to offer significant retention for highly polar compounds even under water rich mobile phases. As illustrated in the following plot, InertSustain AQ-C8 provided exceptional retention for highly polar compounds even under water rich mobile phases without dewetting or phase collapse. Retention Properties of InertSustain AQ-C8 Retention Factor of acidic, basic and neutral compounds using 00% water mobile phase InertSustain AQ-C8 Aquasil C8 Atlantis T Inertsil ODS- Inertsil ODS-SP Inertsil ODS- InertSustain C8 CAPCELL PAK C8-AQ Synergi Fusion RP XSelect CSH C8 Retention Factor of amylbenzene using methanol/water=80/0 Remarks:. The horizontal and vertical retention factor values were calculated with reference to the retention factor values obtained from InertSustain AQ-C8.. The horizontal values represents the retention factor of amylbenzene using methanol/water=80/0.. The vertical values represents the retention factor of acidic, basic and neutral compounds using 00% water mobile phase. (For further details, please see the following chart) InertSustain AQ-C8 provided strong retention for all basic, neutral and acidic compounds under 00% water mobile phase Remarks: -methyluridine Neutral Pyridoxamine Basic Dopamine Generally, the retention for acidic compounds become stronger than basic compounds when the surface of the silica gel is basic. The retention for basic compounds become stronger than acidic compounds when the surface of the silica gel is acidic. InertSustain AQ-C8 Uracil Succinic Acid Acidic Acetic Acid CAPCELL PAK C8-AQ Atlantis T Synergi Fusion RP
3 Highly Polar Compound Retention with 00% Aqueous Mobile Phases It is indeed difficult to retain highly polar samples by reversed phase mode as the polar samples tend to elute near the t 0 (void volume) with water rich mobile phases. On the other hand, polar embedded C8 columns or C8 columns with hydrophilic endcapping are available in the market for retaining highly polar compounds. However, they often show tailing or poor peak shapes due to the secondary interaction from their embedded functional groups. InertSustain AQ-C8 not only provide strong retention for highly polar compounds, but also delivery symmetric peak shapes for virtually any type of analytes. InertSustain AQ-C8 Atlants T Synergi Fusion RP t0 t0 t0 + : Not eluted Column : μm, 0. mm I.D. : 0. % HCO in H O Flow Rate :.0 ml/min Col. Temp. : 0 Detection : UV 0 nm. Pyridoxamine (Vitamin B). Thiamin (Vitamin B). Nicotinic acid (Vitamin B). Pyridoxal (Vitamin B). Nicotinamide (Vitamin B). Pyridoxine (Vitamin B) InertSustain C8 (Conventional C8 column) t Time(min) Retentivity Under 0% Organic Solvent As shown below, InertSustain AQ-C8 delivered stronger retention of catechin sample even under 0% organic solvent mobile phase with exceptional peak shapes, while competitive column brand failed. Furthermore, InertSustain AQ-C8 can prevent the co-elution between the targeted polar analytes and solvent peaks or sample matrices due to its enhanced retentivity. InertSustain AQ-C8 Atlants T Complete separation between solvent peak Elution with solvent peak Poor peak shape Column : μm,0. mm I.D. : A) 0. %HCO in H O B) CH CN A/B= 80/0, v/v Flow Rate :.0 ml/min Col. Temp. :0 Detection :UV 80 nm. Gallocatechin (-) (GC). Catechin (+) (C). Epicatechin (-) (EC). Epigallocatechin gallate (-) (EGCg). Epicatechin gallate (-) (ECg). Catechin gallate (-) (Cg) 0 8 0
4 InertSustain AQ-C8 Applications High-Throughput Analysis of Drugs InertSustain AQ C8 (.9 μm) Acquity BEH C8 (.7 μm).0 (x,000,000)..0. Increase in Sensitivity.0 (x,000,000) min min Column Size Flow Rate Detection Injection Vol. : 0. mm I.D. : A) 0.0 % HCO in H O : B) CH CN A/B= 70/0 - (0. min) - 0/90 - (0. min hold) : 0. ml/min : LC/MS/MS(ESI,SRM) : μl. Propranolol(+). Carbamazepine(+). Salicylic acid(-). Ketoprofen(-) (each 00 μg/l in 0 % ACN) (+): Positive mode (-): Negative mode Analysis of Nucleotide in Fish Meat mau 0 8 mau Column : InertSustain AQ-C8 ( μm, 0. mm I.D.) : 0 mm K HPO in H O (ph 7.0, H PO )* Flow Rate :.0 ml/min Col.Temp. : 0 Detection : UV 0 nm Injection Vol. : μl.imp.atp.adp.amp.hyp.ino (each mg/l) * Equilibrate the column with CH CN/H O=/,v/v after the analysis. When storing the column for a long period of time, store it with 00 % CH CN 00 %.
5 Applications Analysis of Water-Soluble Vitamins.9 μm, 0. mm I.D. 0. ml/min μm, 0. mm I.D.0 ml/min Column Col. Temp. Detection :InertSustain AQ-C8 :A)0.% H PO B) CH CN* :0 :UV 0 nm 8 *Gradient Time Program <.9 μm> Time (min) A% B% < μm> Time (min) A% B% Pyridoxamine. Thiamin. Nicotinic Acid. Ascorbic acid. Nicotinamide. Pyridoxal 7. Pyridoxine 8. Pantonenic acid 9. Folic Acid 0. Cyanocobalamin. Riboflavin. Biotin Analysis of Organic Acids 7 Column : μm, 0. mm I.D. : 0 mm NaH PO in H O (ph., H PO ) Flow Rate :.0 ml / min Col. Temp. :0 Detection :UV 0 nm Inj. Vol. :0 μl Oxalic acid 0 mg/l. Tartaric acid 00 mg/l. Formic acid 000 mg/l. Malic acid 000 mg/l. Lactic acid 000 mg/l. Acetic acid 000 mg/l 7. Maleic acid 0 mg/l 8. Citric acid 000 mg/l 9. Pyroglutamic acid 00 mg/l 0. Succinic acid 000 mg/l. Fumaric acid 0 mg/l 0 8
6 InertSustain AQ-C8 Applications Analysis of Catecolamines Column : μm, 0. mm I.D. : 0.% H PO in H O Flow Rate :.0 ml / min Col. Temp. :0 Detection :UV 0 nm Inj. Vol. : μl. Norepinephrine. L-Adrenaline. Dopamine. L-DOPA. L-(-)-Tyrosine HO NH HO N H NH HO O O HO NH HO NH Analysis of Fish Freshness, K-Value Column : μm, 0. mm I.D. : 0 mm NaH PO in H O Flow Rate :.0 ml / min Col. Temp. :0 Detection :UV nm Inj. Vol. : μl Sample. ATP. ADP. IMP. Hypoxanthine. AMP. Inosine :00 ppm 0 0 0
7 Applications Analysis of β blocker Column : μm, 0. mm I.D. : A: CH CN B: 0.% H PO in H O A/B=/7, v/v Flow Rate :.0 ml / min Col. Temp. :0 Detection :UV 0 nm Inj. Vol. : μl. Atenolol. Acebutolol. Oxprenolol. Labetalol. Propranolol. Alprenolol (00 μg/ml each) Analysis of Nucleoside and Nucleic Acid Base Column : μm, 0. mm I.D. : 0. M KH PO in H O + 0. M NaClO in H O (ph.0, H PO ) Flow Rate :.0 ml / min Col. Temp. :0 Detection :UV 0 nm Inj. Vol. : μl mau Cytosine. Uracil. Guanine. Adenine. Cytidine. Uridine 7. Thymine 8. Adenosine 9. Guanosine 0. Thymidine (00 mg/l each)
8 InertSustain AQ-C8 Applications Analysis of Nucleotides via LC/MS/MS Samples such as nucleotides have several phosphate groups which is sensitive to stainless steel hardware. As shown below, the combination of highly inert packing material of InertSustain AQ-C8 and usage of a new *Steel- Coated-PEEK hardware (metal-free) deliver excellent peak shapes with higher sensitivity for phosphate compounds WITHOUT the formation of phosphate-iron complexes found with stainless steel column hardwares. *Ordering information on Steel-Coated-PEEK hardware is available at page 0. System Column size : Nexera LCMS-800 plus system : μm HP, 0 x. mm I.D : A) mm Ammonium formate in H O B) CH CN Flow Rate : 0. ml/min Col. Temp. : 0 Detection : LC/MS/MS (ESI), Positive Injection Vol. : μl B% Sample :.ATP 00 μg/l.adp 00 μg/l.amp 00 μg/l Steel-Coated-PEEK hardware Packed with InertSustain AQ-C8 Stainless Steel Hardware Packed with InertSustain AQ-C8 PEEK Packing Material Packing Material Stainless Steel Stainless Steel.0 (x0,000) RT=. min..0 (x0,000). RT=. min Adsorption to the stainless steel hardware.0. Higher Sensitivity without adsorption t0 0. t min min 8
9 Ordering Information Analytical Columns Particle Size:.9 μm 80 MPa (800 Bar) End-fittings are UP Type end-fittings. Length/ I.D. (mm) End-fitting Format. mm UP Type End-fittings HP Series Particle Size: μm 0 MPa (00 Bar) Length/ I.D. (mm) End-fittings are / Waters-compatible. UHPLC compatible end-fittings are also availa ble upon request for UHPLCsystems (Ex: UPLC )to avoid dead volume. Indicate UP Type end-fittings when ordering. (Please note that UP type is not available for a. mm I.D. column). mm / Waters End-fittings UPLC is a registered trademark of Waters Corporation. Particle Size: μm 0 MPa (00 Bar) Particle Size: μm 0 MPa (00 Bar) Length/I.D. (mm) Length/I.D. (mm) Length/I.D. (mm) Length/I.D. (mm) End-fittings are / Waters-compatible. Other column sizes available upon request. 9
10 InertSustain AQ-C8 Ordering Information Guard Column for UHPLC (Max. Operating Pressure 80 MPa, 800 Bar) I.D. of the Analytical Column Applicable (mm).0 Length (mm) I.D. (mm) Replacement Cartridge ( pcs) For connection details, please refer to the diagram below. Cartridge ( pcs) + Holder ( pcs) Set Replacement Cartridge ( pcs) Cartridge ( pcs) + Holder ( pcs) Set Particle Size.9 μm Particle Size.9 μm Particle Size μm Particle Size μm , , Holder for Guard Column for UHPLC Instrument Side / (No. 0- UNF) Holder PEEK Guide Packing Connection to Analytical Column Cartridge Guard Column / O.D. x 0.8 mm I.D. x 0 mm tubing Cartridge Guard Columns I.D. of the Analytical Column Applicable (mm).0 Length (mm) I.D. (mm) End-fittings are / Waters-compatible. Maximum operating pressure is 0 MPa, 00 Bar. Replacement Cartridge E ( pcs) Particle Size Cartridge E ( pcs) + Holder ( pcs) Set Particle Size μm μm μm μm , , , , , Holder for Cartridge Guard Column E Preparative Columns For 0 mm Length For 0 mm Length Particle Size: μm 0 MPa (00 Bar) Length/ I.D. (mm) Preparative Guard Columns Particle Size: μm 0 MPa (00 Bar) Length x I.D. (mm)
11 Ordering Information Capillary Columns EX-Nano Columns Particle Size: μm MPa (0 Bar) EX Columns Particle Size: μm 0 MPa (00 Bar) EX-Nano Columns Particle Size: μm MPa (0 Bar) EX Columns Particle Size: μm 0 MPa (00 Bar) Length/ I.D. (mm) Length/ I.D. (mm) Length/ I.D. (mm) Length/ I.D. (mm) End-fittings are / Valco (0- UNF). / Valco (-0 UNF) end-fittings ca n also be arranged upon request. Indicate / end-fittings when ordering. Connection Kits for Capillary EX-Nano and EX Columns Column Coupler mm I.D. / O.D. Tubing (Both ends with male nuts including PEEK ferrules) Capillary Tubing Connector mm I.D. 0.7 mm O.D. Tubing mm I.D. 0.7 mm O.D. Tubing mm I.D. 0.7 mm O.D. Tubing (Male nut, PEEK ferrule, / O.D. PTFE with sleeve) For Capillary EX-Nano Columns PTFE Tubing 0 mm pcs / O.D. (O.D. 0.7 mm Connection for Capillary Tubing) Column Coupler 0 0. mm I.D. / O.D.Tubing (Both ends with male nuts including PEEK ferrules) Capillary Tubing Connector mm I.D. 0.7 mm O.D. Tubing (Male nut, PEEK ferrule, / O.D. PTFE with sleeve) For Capillary EX Columns PTFE Tubing 0 mm pcs / O.D. (O.D. 0.7 mm Connection for Capillary Tubing)
12 InertSustain AQ-C8 Ordering Information Metal-Free PEEK Columns PEEK Columns Particle Size: μm 0 MPa (00 Bar) Steel-Coated PEEK Columns Particle Size: μm 0 MPa (00 Bar) Length/ I.D. (mm).. 0 Please inquire - Please inquire Please inquire 0 Please inquire Please inquire 7 - Please inquire 00 Please inquire Please inquire 0 Please inquire Please inquire 00 - Please inquire 0 Please inquire Please inquire 00 Please inquire Please inquire Length/ I.D. (mm).. 0 Please inquire Please inquire 00 Please inquire Please inquire 0 Please inquire Please inquire 0 Please inquire Please inquire End-fitting Format. mm UP Type End-fittings End-fittings are UP type end-fittings. For further product details, please contact your local GL Sciences representative. To order: Tel: + (0) sales@hichrom.co.uk
Method Development Kits
Method Development Kits sales representative for ordering information or contact...77 Method Development Kits First column choice for Pharma Development USP L C Method Development Kits Maximize retention
More informationAn HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint
An HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint An important new column in the Inertsil series Bonded-Phase Structure Perfect balance of retention for both polar
More informationInertSustainSwift C8
HPLC, LC/MS Columns InertSustainSwift TM C8 New! InertSustainSwift C8 is an octyl group (C8) bonded column offering the same extreme inertness to any type of compounds just like InertSustainSwift C8, which
More informationThe Evolving HPLC Column Packings
The Evolving HPLC Column Packings GL Sciences has been steadily supplying with columns from Inertsil ODS, first-generation to InertSustain series, integration of state-ofthe-art technologies, and has established
More informationState-of-the-art C18 HPLC Columns
An HPLC GL Sciences Newest and Most Advanced ODS Phase-New For 00 State-of-the-art C HPLC s Improved Peak Shapes and Heights Enhancing Sensitivity High Resolution Fast Equilibration Compatible with 00%
More informationInertSustainSwift C8
Physical Properties Silica Particle Size Surface Area Pore Size Pore Volume Bonded Phase End-capping Carbon Loading ph Range USP Code :ES (Evolved Surface) Silica Gel :.9 μm, μm, μm :00 m /g :00 Å (0 nm)
More informationSunniest C18 Sunniest RP-AQUA Sunniest C8 Patent pending
Patent pending Website : www.chromtech.net.au E-mail : info@chromtech.net.au telno : 0 97 0... in AUSTRALIA A Novel Bonding Technique (patent pending) An unique trifunctional silyl-reagent was developed
More informationIon Exchange Columns ---- Inertsil AX 078 Inertsil CX 080. HPLC Column Catalog
Ion Exchange ---- Inertsil AX 078 Inertsil CX 080 HPLC Column Catalog 077 Inertsil AX Physical Properties Silica : Series High Purity Silica Gel : μm Surface Area : 0 m /g Pore Size : 00 Å (0 nm) Pore
More informationThe Selection of an HPLC Column October
The Selection of an HPLC Column 07. ctober Long-Term Collaboration + 30 Years of Experience GL Sciences Inc. HPLC Selection of an HPLC Column 3 Contents. How to Select an HPLC Column How to select your
More informationAN HPLC COLUMN InertSustain C1 AN HPLC COLUMN InertSustain C1 Inertsil continues to evolve to InertSustain Physical Properties
AN HPLC COLUMN TM InertSustain C8 AN HPLC COLUMN InertSustain TM C8 Inertsil continues to evolve to InertSustain Physical Properties Silica : Newly Developed Silica Gel Particle Size : m, m, m Surface
More informationIt s hot! ZIC -chilic
It s hot! ZIC -chilic Complementary selectivity for HPLC and LC-MS separation of polar hydrophilic compounds EMD Millipore Corp. is a subsidiary of Merck KGaA, Darmstadt, Germany ZIC -chilic Your benefits
More informationToo Polar for Reversed Phase What Do You Do?
Too Polar for Reversed Phase What Do You Do? June 20, 2013 Mark Powell Columns and Consumables Technical Support Page 1 C8 or C18 Doesn t Always Do the Job Typical reversed phase conditions involve water/buffer
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS SeQuant ZIC-HILIC. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS SeQuant ZIC-HILIC Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 PE, UK Tel: + (0)8 90 660 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationState - of - the -art C18 HPLC Columns
An HPLC GL Sciences Newest and Most Advanced ODS Phase -New For State - of - the -art C HPLC s Improved Peak Shapes and Heights Enhancing Sensitivity High Resolution Fast Equilibration Compatible with
More informationPacked columns for reversed-phase chromatography TSKgel ODS-100V and 100Z
Packed columns for reversed-phase chromatography TSKgel ODS-100V and 100Z Contents Page 1. Introduction 2 2. Specifications and characteristics 2 3. Properties of packing material 3 4. Chromatographic
More informationInertsil Technical Library
HPLC COLUMN INERTSIL TECHNICAL LIBRARY H P L C C o l u m n Inertsil Technical Library Inertsil ODS-4, C8-4 Comparison of Performance List of Compared Columns Experimental Explanation, Analytical Conditions
More informationSmall Molecule Selectivity Sampler
Small Molecule Selectivity Sampler HALO 90 Å C18, AQ-C18, BIPHENYL Fused-Core Particle Technology Why Choose Fused-Core Particle Technology? Fused-Core Out-Performs Totally Porous Particles. HALO particles
More informationAQUASIL C18 Columns. AQUASIL 18 Columns TG Compatible with 100% aqueous mobile phase
AQUASIL C8 Columns AQUASIL 8 Columns TG0-0 Compatible with 00% aqueous mobile phase AQUASIL OH OH AQUASIL C8 columns provide a versatile C8 phase for a wide range of application areas. AQUASIL C8 columns
More informationPart 2. Overview of HPLC Media
Part 1. General Chromatographic Theory Part 2. Overview of HPLC Media Part 3. The Role of the Mobile Phase in Selectivity Part 4. Column Care and Use 1 HPLC Particle Technology Core-Shell Particle Fully
More informationThermo Scientific Accucore XL HPLC Columns. Technical Manual
Thermo Scientific Accucore XL HPLC Columns Technical Manual Thermo Scientific Accucore XL HPLC Columns Based on Core Enhanced Technology using µm solid core particles, Accucore XL HPLC columns allow users
More informationAlltech Alltima HP Introduction
Alltech Introduction High-Stability, High-Purity, High-Performance, Low-Bleed Columns for Demanding Applications Better Peak Symmetry high-purity silica eliminates peak tailing problems Long Column Life
More informationColumnsAcclaim PolarAdvantage II (PA2) HPLC Columns
ColumnsAcclaim PolarAdvantage II (PA) HPLC Columns A Unique and Complementary Alternative to Conventional C8.8 Column: Acclaim PA, µm Peaks: ( ppm each) Dimensions:. mm. Maleate Mobile Phase: MeOH/.% NH
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Zorbax. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Zorbax Catalogue 9 1 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484 Email:
More informationpenta-hilic UHPLC COLUMNS
penta-hilic UHPLC COLUMNS penta-hilic Highly retentive, proprietary penta-hydroxy-ligand Excellent peak shape for polar compounds with a variety of functional groups: acids, bases, zwitterions strong and
More informationAnalyze Nucleotides, Nucleosides, Purine, and Pyrimidine Bases Simultaneously with the Ultra IBD Column
pharmaceutical #9 Applications note Analyze Nucleotides, Nucleosides, Purine, and Pyrimidine Bases Simultaneously with the Ultra IBD Column Mixtures of nucleotides, nucleosides, and their respective purine
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS SIELC Primesep. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS SIELC Primesep Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)8 90 660 Fax: +44 (0)8 9 484 Email: sales@hichrom.co.uk
More informationpenta-hilic UHPLC COLUMNS
penta-hilic UHPLC COLUMNS Highly retentive, proprietary penta-hydroxy-ligand penta-hilic Excellent peak shape for polar compounds with a variety of functional groups: acids, bases, zwitterions strong and
More informationDo you need trouble-free speed? The NEW Chromolith HR
Do you need trouble-free speed? The NEW Chromolith HR EMD Millipore is a division of Merck KGaA, Darmstadt, Germany ... then speed-up to Chromolith HighResolution... Revolutionary monolithic silica columns
More informationLC800. Smart HPLC. Until now UHPLC From now Smart HPLC
LC800 Smart HPLC Until now UHPLC From now Smart HPLC Smart HPLC Leads to Ultimate Performance Patent Pending S LC800 is a completely new and unique concept, designed for maximum performance in high resolution
More informationDo you need trouble-free speed? The NEW Chromolith HR
Do you need trouble-free speed? The NEW Chromolith HR EMD Millipore is a division of Merck KGaA, Darmstadt, Germany ... then speed-up to Chromolith HighResolution... Revolutionary monolithic silica columns
More informationShodex TM ODP2 HP series columns
HPLC Columns Shodex TM ODP2 HP series columns Better retention of highly polar substances Technical notebook No. 6 Contents 1. Introduction 1-1. Specifications 1-2. Eluent Compatibility of ODP2 HP Series
More informationACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development
ACE C8-Amide For increased polar retention and alternative selectivity Alternative selectivity for method development Improved separations with polar, acidic, basic and phenolic compounds High efficiency
More informationACE Excel SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE Excel SuperC8 TM Ultra-Inert UHPLC and HPLC s with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS-compatible buffers between ph.. High efficiency µm, µm,
More informationProduct Bulletin. ACE LC/MS and Rapid Analysis HPLC Columns. HPLC Columns
Product Bulletin HPLC Columns ACE LC/MS and Rapid Analysis HPLC Columns 0 mm, 30 mm, 35 mm and 50 mm column lengths.0,., 3.0, 4.0 and 4.6 mm column diameters Configured for High Sample Throughput Specially
More informationACE SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE SuperC8 TM Ultra-Inert UHPLC and HPLC s with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS compatible buffers between ph.. High efficiency µm, µm, µm and
More informationHPLC column. Sunniest. C18, C18-HT, RP-AQUA, C8, PhE, PFP. ChromaNik Technologies Inc.
HPLC column Sunniest C8, C8-HT, RP-AQUA, C8, PhE, PFP Chromaik Technologies Inc. A ovel Bonding Technique (patent pending) The State of Art trifunctional silyl-reagent was developed as shown in Fig.. This
More informationSTUDY OF LOADABILITY AND SELECTIVITY OF PHARMACEUTICAL COMPOUNDS ON RPLC COLUMNS
STUDY OF LOADABILITY AND SELECTIVITY OF PHARMACEUTICAL COMPOUNDS ON RPLC COLUMNS Fang Xia, Jie Y. Cavanaugh, Uwe Neue, Jeffrey R. Mazzeo, Diane M. Diehl Waters Corporation, Milford, MA USA OVERVIEW- INTRODUCTION-
More information1.7 m Fortis UHPLC Columns
Strength in Technology 1.7 m Fortis Columns Ultra High Pressure Chromatography Improve your Performance 8 Chemistry Choices Increase Efficiency Increase Speed Improve Resolution Greater Sensitivity Lower
More informationThe Secrets of Rapid HPLC Method Development. Choosing Columns for Rapid Method Development and Short Analysis Times
The Secrets of Rapid HPLC Method Development Choosing Columns for Rapid Method Development and Short Analysis Times Rapid Analysis Is More Than Run Time It is developing a method to meet a goal and developing
More informationcolumns Acclaim Mixed-Mode WCX-1 for Separating Basic Molecules
columns Acclaim Mixed-Mode WCX- for Separating Basic Molecules The Acclaim Mixed-Mode WCX- is a novel, high-efficiency, silica-based column specially designed for separating various basic analytes. This
More informationPackings for HPLC. Packings for HPLC
Summary of packings for HPLC In analytical HPLC, packings with particle sizes of 3 to 10 µm are preferred. For preparative separation tasks, also particles with diameters larger than 10 µm are applied.
More informationHPLC UHPLC Columns. Dikma Technologies Inc.
Technologies Inc. About HPLC UHPLC Columns has over years of R&D experience in HPLC / UHPLC columns. HPLC / UHPLC columns have been widely used by pharmaceutical manufacturers. We offer columns of ultimate
More informationZirconia: the Ideal Substrate for Ion-Exchange LC and LC-MS
Zirconia: the Ideal Substrate for Ion-Exchange LC and LC-MS EAS 2005 Bingwen Yan 1, Clayton V. McNeff 1, Richard A. Henry 2, and David S. Bell 2 1 ZirChrom Separations, Inc., 617 Pierce Street, Anoka,
More informationImpSilHPLC Columns. The solution for your chromatography IMPTECH SCIENTIFIC
ImpSilHPLC Columns The solution for your chromatography IMPTECH SCIENTIFIC 7-1-60,203-Veena Dharam Karam Road,Ameerpet,Hyderabad-500 016 Tel:040-23740443/483 Ph:9848051071 imptechscientific@gmail.com www.impscience.com
More informationzorbax Packed by Phenomenex Material Characteristics High efficiency Guaranteed quality Phenomenex columns packed with Zorbax
Packed by Phenomenex High efficiency Guaranteed quality Phenomenex columns packed with 7 μm materials offer the advantages of lower cost as well as lower operating backpressures, which result in long column
More informationLC Technical Information
LC Technical Information Method Transfer to Accucore.6 μm Columns Containing solid core particles, which are engineered to a diameter of.6μm and a very narrow particle size distribution; Accucore HPLC
More informationINERTSIL (GL Sciences)
Inertsil 3 Series The Inertsil 3 series from GL Sciencesʼ represents a major advance in performance over the original Inertsil 2 Series. Inertsil 3 Series phases are based on a purer, higher surface area
More informationSample Preparation TLC Plates
TLC PLATES TLC Plates Economical separation method High sample throughput Pilot procedure for HPLC and flash chromatography Versatile range of ready-to-use layers Thin layer chromatography (TLC) is a simple,
More informationMethod Development with ZirChrom's Ion Exchange Phases
For Peak Performance ZIRCHROM ION EXCHANGERS Phases for Sugars and Proteins Wide Range of Ion Exchange Selectivity No Shrinking or Swelling Use Any Organic Solvent Significantly Higher Efficiency than
More informationPut the. C18 aside. and take a look... Reasons. to try the. NEW Gemini C6-Phenyl
Put the C18 aside and take a look... 6 Reasons to try the EW Gemini Reason #6 ALTERATE SELECTIVITY to your C18 Column Adding to your method development options, Gemini offers additional polar and aromatic
More informationInertsil ODS-EP Technical Information
Technical Information Advantages of The selectivity is completely different from those of conventional columns such as ODS column due to its specific polar group in the stationary phase. Durability can
More informationOptimisil HPLC & UPLC Column catalogue
Optimisil HPLC & UPLC Column catalogue Analytical Hub Plot No: 37, P & T Colony, Vikrampuri, Secunderabad 500009 Tel: 040-64621313, Mobile: +91 7799931313 Website: www.analyticalhub.com email: contact@analyticalhub.com
More informationISOSCELES PELLICULAR CORE
ISSCELES PELLICULAR CRE Isosceles Chemistry Phases Isosceles C8 USP Classification L Isosceles C8 USP Classification L7 Isosceles AQ USP Classification equivalent to L6 Isosceles Amide (ILIC) USP Classification
More informationFast Analysis of Small MW Analytes in a Beverage and Serum Samples on a Zirconiabased Strong Anion-Exchanger
Fast Analysis of Small MW Analytes in a Beverage and Serum Samples on a Zirconiabased Strong Anion-Exchanger Pittcon 2005 BINGWEN YAN 1, CLAYTON V. MCNEFF 1, 1 ZirChrom Separations, Inc., 617 Pierce Street,
More informationShim-pack GIST/GISS/GIS/GWS Series
and Normal Phase HPLC Shim-pack GIST/GISS/GIS/GWS Series C0-EB Shimadzu Liquid Chromatographs Maximizing the Potential of UHPLC/HPLC Analysis Meet the Nexera X, the most advanced UHPLC available today.
More informationAdvantages of polymerbased. an alternative for ODS
Advantages of polymerbased HPLC columns an alternative for ODS Yukiko Higai Shodex, 1 Content (1) Comparison between Silica and Polymer-based RP columns (2) Characteristics of Polymer RP columns - an example:
More informationA Look at Agilent HPLC Columns Choices
A Look at Agilent HPLC Columns Choices Brno & Prague, May 29-30 2018 Giorgio Ferlat IDO Product Specialist Chemistries and Consumable 1 Considerating Column Properties for Column Selection Silica Particle
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Inertsil. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Inertsil Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 PE, UK Tel: + (0)8 90 660 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationC18 Column. Care & Use Sheet
C18 Column Care & Use Sheet HALO Description HALO C18 is a high-speed, high-performance liquid chromatography column based on a new Fused-CoreTM particle design. The Fused-Core particle provides a thin
More informationLC Columns with Liquid Separation Cell Technology
Obelisc LC Columns with Liquid Separation Cell Technology Obelisc HPLC Columns - Liquid Separation Cell Technology Introduction Obelisc HPLC columns are the latest, innovative columns from SIELC Technologies,
More informationSuccessfully Scaling and Transferring HPLC and UPLC Methods
Successfully Scaling and Transferring HPLC and UPLC Methods Esa Lehtorinne Esa_Lehtorinne@waters.com Tel: +358-9-5659 6288 Fax: +358-9-5659 6282 Waters Finland Kutomotie 16 00380 Helsinki 2013 Waters Corporation
More informationWelcome to our E-Seminar: Choosing HPLC Columns for Faster Analysis Smaller and Faster
Welcome to our E-Sear: Choosing HPLC Columns for Faster Analysis Smaller and Faster High Throughput/Fast LC Requires. Short columns 0 mm or shorter Small particle sizes. µm Rapid Resolution or new.8 µm
More informationACE SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE SuperC8 TM Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS compatible buffers between ph.. High efficiency µm, µm,
More informationfor Acclaim Mixed-Mode HILIC-1 Column
for Acclaim Mixed-Mode HILIC-1 Column Product Manual for ACCLAIM Mixed-Mode HILIC-1 Page 1 of 17 Product Manual for ACCLAIM Mixed-Mode HILIC-1 Column 5µm, 4.6 x 250mm, P/N 066844 5µm, 4.6 x 150mm, P/N
More informationACE C18-PFP. Hydrophobic and pentafluorophenyl mixed mode interaction. High efficiency 2µm, 3µm, 5µm and 10µm particles for UHPLC and HPLC
ACE C-PFP A C bonded phase with unique selectivity F F F F F Guaranteed reproducibility Exceptional bonded phase stability Hydrophobic and pentafluorophenyl mixed mode interaction High efficiency µm, µm,
More informationGemini. Second-Generation TWIN-NX Technology. TWIN (Two-In-One) Technology. U.S. Patent No. 7, 563, 367 Setting the Standard for ph Method Development
U.S. Patent o. 7, 5, 7 Setting the Standard for ph Method Development ugged reversed phase HPLC columns that offer extended lifetime at extreme ph conditions and excellent stability for reproducible, high
More informationSetting the Standard for ph Method Development
U.S. Patent o. 7, 5, 7 Setting the Standard for ph Method Development ugged reversed phase HPLC columns that offer extended lifetime at extreme ph conditions and excellent stability for reproducible, high
More informationCAPCELL PAK C18 IF2 Type http://hplc.shiseido.co.jp/e/column/html/if2_index.htm Page 1 of 2 List of Sales Representatives Contact Shiseido Technical Materials Catalog List HPLC Columns HPLC Instruments
More informationThermo Scientific Syncronis HPLC Columns. Remarkable separations guaranteed time after time
Thermo Scientific Syncronis HPLC Columns Remarkable separations guaranteed time after time Syncronis HPLC and UHPLC Columns Remarkable separations guaranteed time after time When developing a new method,
More informationNew Product Update. HPLC Columns
New Product Update HPLC Columns ZORBAX MicroBore HPLC Columns ZORBAX Extend-C18 Columns ZORBAX Bonus-RP Columns Inertsil ODS-2 Cartridge Columns ZORBAX Carbohydrate Analysis Columns ZORBAX Eclipse dsdna
More informationOBSERVATIONS ON THE WETTING OF REVERSED-PHASE HPLC PACKINGS
1997 Waters Corporation p. 1 980947 OBSERVATIONS ON THE WETTING OF REVERSED-PHASE HPLC PACKINGS T. Walter, P. Iraneta, and M. Capparella Waters Corporation, 34 Maple Street, Milford, MA 01757 USA Presented
More informationThe Analysis of Organic Acids in Foods and Beverages Using Reversed Phase HPLC
The Analysis of rganic Acids in Foods and Beverages Using Reversed Phase HPLC Rebecca E. Wittrig & Terrence S. Reid Restek Corporation Benner Circle, Bellefonte, PA 683 USA Restek Corporation www.restekcorp
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS HALO and HALO-5. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS HALO and HALO- Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG PE, UK Tel: + (0)8 90 0 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationNovel LC/MS-Compatible Stationary Phase with Polar Selectivity
Novel LC/MS-Compatible Stationary Phase with Polar Selectivity Carmen T. Santasania and David S. Bell Supelco 595 N. Harrison Rd., Bellefonte, PA 16823 T403168 GJU Introduction Stationary phases based
More informationCation Exchange HPLC Columns
Cation Exchange PRP-X00 Polymeric cation exchange packing for separation of inorganic cations and organic cations. Easily separate mono or divalent cations. ph stable from to. Use with organic solvent
More informationZirconia-Based Phases as a Powerful Complement to Silica-Based Phases for LC and LC-MS under Non-extreme Mobile Phase Conditions
Zirconia-Based Phases as a Powerful Complement to Silica-Based Phases for LC and LC-MS under on-extreme Mobile Phase Conditions Richard A. Henry, Shawn R. Wyatt, Carmen T. Santasania and David S. Bell
More informationOrdering and Warranty Information
Ordering and Warranty Information In the U.S.A. All Orders Including: Credit Card Orders (VISA, American Express, MasterCard) Blanket Orders Emergency Orders Call -8-Dionex- (-8-6-69) or FAX -8-76-76 Outside
More informationLC and LC/MS Column Selection Flow Chart
LC and LC/MS Column Selection Flow Chart To use the column selection diagram below, simply follow the path for your analyte and mobile phase. At the far right, follow your final column selection to the
More informationHPLC Column. Technical Guide
HPLC Column Technical Guide (1) Introduction P 1 (2) Specifications P 1 ~ 4 (3) Shipping Solvent P 5 ~ 8 (4) Mobile Phase P 9 ~ 11 (5) Sample Diluent/Injection Solvent P 12 (6) Column Cleaning and Storage
More informationKromasil Eternity Designed for long life
Kromasil Eternity Designed for long life For use in HPLC and UHPLC Kromasil The long-lasting phase for demanding applications Imagine working under virtually no ph restrictions during method development.
More informationPacked Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS. Table of Contents
No. 089 SEPARATION REPORT Packed Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS Table of Contents 1. Introduction 1 2. Column Specification 1 3. Features of Packing Materials
More informationKromasil Eternity Designed for long life. For use in HPLC and UHPLC
Kromasil Eternity Designed for long life For use in HPLC and UHPLC The long-lasting phase for demanding applications Imagine working under virtually no ph restrictions during method development. That s
More informationACE Ultra Inert Base Deactivated HPLC Columns
ACE Ultra Inert Base Deactivated HPLC Columns Contents Page ACE HPLC Columns...- Independent Comparison of HPLC Columns #... Independent Comparison of HPLC Columns #... ACE 00Å HPLC Columns Specifications...-
More informationBETASIL Columns TG Å high purity silica
BETASIL Columns TG0-0 00Å high purity silica Technical Guide BETASIL Columns Introduction 00Å high purity silica High surface area and phase loading Designed for small molecules Type B silica gives good
More informationPassion. Power. Productivity.
- - Passion. Power. Productivity. Acclaim Acclaim Acclaim PA/PA Acclaim A Acclaim Acclaim PA Acclaim Explosives rdering and Warranty Information In the U.S. All rders Including: Credit Card rders (VISA,
More informationSetting the Standard for ph Method Development
U.S. Patent o. 7, 56, 67 Setting the Standard for ph Method Development ugged reversed phase HPLC columns that offer extended lifetime at extreme ph conditions and excellent stability for reproducible,
More informationStationary phases for prep LC
The Selectivity Company Versatile hybrid silica-based Stationary phases for prep LC YMC-Triart Prep Stability Reliability ph stable www.ymc.de 2 Contents 3 page Introduction...4-8 How to Make the Correct
More informationFast Separation of Vastly Different Compounds by Isocratic HPLC
Fast Separation of Vastly Different Compounds by Isocratic HPLC Aimee N. Heyrman and Yury Zelechonok Resolution Systems, Inc. 590 E. 32nd St. Holland. MI, 49423 SIELC Technologies, 15 E. Palatine Rd, Suite
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Vydac. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Vydac Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG PE, UK Tel: + (0)8 90 660 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationHPLC column C18 C8 PFP. SunShell. Core Shell Particle
HPLC column C8 C8 PFP SunShell Core Shell Particle ChromaNik Technologies Inc. SunShell is a core shell silica column made by ChromaNIk Technologies. The next generation to Core Shell particle Features
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Thermo Scientific Acclaim. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Thermo Scientific Acclaim Catalogue The Markham Centre, Station Road Theale, Reading, Berks, RG PE, UK Tel: + (0) 0 0 Fax: + (0) Email: sales@hichrom.co.uk
More informationNew 5-micron HALO-5 columns based on Fused- Core particle technology boost the performance of HPLC. Compared to other HPLC columns, HALO-5 columns
New 5-micron HALO-5 columns based on Fused- Core particle technology boost the performance of HPLC. Compared to other HPLC columns, HALO-5 columns have: the highest plate number versus any other 5-micron
More informationMethod Development in Solid Phase Extraction using Non-Polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples
Technical Note 101 Method Development in Solid Phase Extraction using Non-Polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples This technical note includes by specific information on the extraction
More informationA Quality by Design (QbD) Based Method Development for the Determination of Impurities in a Peroxide Degraded Sample of Ziprasidone
A Quality by Design (QbD) Based Method Development for the Determination of Impurities in a Peroxide Degraded Sample of Ziprasidone Mia Summers and Kenneth J. Fountain Waters Corporation, 34 Maple St.,
More informationfor Acclaim Mixed-Mode WCX-1
for Acclaim Mixed-Mode WCX- Product Manual for the Acclaim Mixed-Mode WCX- Column Page of 8 Product Manual for Acclaim Mixed-Mode WCX- Columns Acclaim Mixed-Mode WCX-, 3µm, Analytical column, 3.0 x 50mm
More informationTrends in Method Development for HPLC and UHPLC... the movement toward using solid-core particles
Trends in Method Development for HPLC and UHPLC... the movement toward using solid-core particles Richard A. Henry, Separation Technology Consultant On behalf of: Supelco/Sigma-Aldrich, Bellefonte, USA
More informationAcclaim Mixed-Mode WCX-1
Acclaim Mixed-Mode WCX-1 Product Manual for the Acclaim Mixed-Mode WCX-1 Column Page 1 of 28 PRODUCT MANUAL for the Acclaim Mixed-Mode WCX-1 Columns 4.6 x 150 mm, P/N (068353) 4.6 x 250 mm, P/N (068352)
More informationYour Reversed Phase Method
PLC Columns for Your Reversed Phase Method Balanced Non-Polar & Polar Selectivity Strong Non-Polar & Polar Selectivity Strong Polar & Aromatic Selectivity Unique Isomeric & alogenated Selectivity Robust
More informationTechnical Guide Thermo Scientific Syncronis HPLC Columns
Technical Guide Thermo Scientific Syncronis HPLC Columns Consistent, predictable separations, Column after column, time after time Thermo Scientific Syncronis Columns When developing a new method, one
More informationStreamlining the Analysis of Oral Contraceptives Using the ACQUITY UPLC H-Class System
Streamlining the Analysis of Oral Contraceptives Using the ACQUITY UPLC H-Class System Margaret Maziarz, Sean M. McCarthy, Michael D. Jones, and Warren B. Potts Waters Corporation, Milford, MA, USA A P
More information