NUCLEODUR (Macherey-Nagel)
|
|
- Bernadette Palmer
- 5 years ago
- Views:
Transcription
1 NUCLEDUR (Macherey-Nagel) Ultrapure silica Excellent mechanical and chemical stability Low metal content Enhanced ph stability Manufactured by Macherey-Nagel GmbH & Co. KG, Düren, Germany, NUCLEDUR is a fully synthetic type silica offering highly advanced physical properties. The special surface bonding technology and the low concentration of metal ions lead to high efficiency symmetrical peaks. NUCLEDUR Phase Functional Group Endcapped Particle ze (µm) Pore ze (Å) Surface rea (m 2 /g) Carbon Load (%) C18 Gravity ctadecyl Yes 1.8, 3, C18 ec ctadecyl Yes 3, C8 Gravity ctyl Yes 1.8, C8 ec ctyl Yes 3, C18 Pyramid ctadecyl Yes 1.8, 3, C18 Isis ctadecyl Yes 1.8, 3, C18 HTec ctadecyl Yes 1.8, 3, 5, 7, CN / CN-RP Cyano Yes 3, HILIC mmonium-sulphonic acid No 1.8, 3, NH 2 / NH 2 -RP mino Yes 3, PFP Pentafluorophenyl propyl modification Yes 1.8, 3, PolarTec C18 modification with embedded polar group Yes 1.8, 3, Sphinx RP ctadecyl/phenyl No 1.8, 3, NUCLEDUR Gravity NUCLEDUR C18 Gravity and C8 Gravity are based on the ultrapure NUCLEDUR silica. The high density bonding of these phases leads to an extended operation ph range (1-11). This can be helpful for improving selectivity in method development and for more challenging applications. NUCLEDUR C18 ec and C8 ec The NUCLEDUR C18 ec and C8 ec phases are recommended for routing analyses and for scaling up to preparative LC. NUCLEDUR C18 Isis NUCLEDUR C18 Isis is a C18 bonded phase with specially cross-linked surface modification. This polymeric bonding leads to high steric selectivity and is therefore suited for separation of positional and structural isomers and planar/non-planar molecules. The phase has an enhanced ph stability of The separation of o-terphenyl and triphenylene is a concrete example to evaluate the selectivity potential of a reversed-phase column in terms of the different shape of two molecules. The phenyl rings of o-terphenyl are twisted out of plane while triphenylene has a planar geometry (see Figure 1). NUCLEDUR C18 Pyramid NUCLEDUR C18 Pyramid is a C18 bonded phase with hydrophilic endcapping designed especially for use with eluents containing up to 100% water. Figure 2 shows the retention behaviour of tartaric, acetic and maleic acids under purely aqueous conditions on NUCLEDUR C18 Pyramid in comparison with a conventionally bonded RP phase. Figure 2. Stability test Figure 1. Steric selectivity of NUCLEDUR C18 Isis NUCLEDUR NH 2 The aminopropyl modification of the NUCLEDUR NH 2 phase enables the column to be used in reversed and normal-phase modes. The polarity of the phase can be classified as intermediate, based on multiple retention mechanisms such as dipoledipole and hydrophobic. NUCLEDUR NH 2 shows excellent selectivity for polar organic compounds, such as sugar, anilines and esters. Toll Free: info@lifescience.ca Web: 187
2 NUCLEDUR (Macherey-Nagel) verview of NUCLEDUR Phases Phase Specification Characteristics* Stability Structure octadecyl phase, high density coating multi-endcapping C 18 Gravity: 18 % C USP L1 Gravity: 11 % C USP L7 C 18 C 18 C C 18 ph stability 1-11, octadecyl phase with specially crosslinked surface modification endcapping 20 % C USP L1 C ph stability 1-10, C 18 modification with polar endcapping 14 % C USP L1 C stable against 100 % aqueous eluents, ph stability 1-9, bifunctional RP phase, propylphenyl and C 18 ligands; endcapping 15 % C; USP L1 and L11 C ph stability 1-10, octadecyl / octyl phase, medium density coating endcapping C 18 ec: 17.5 % C USP L1 ec: 10.5 % C USP L7 C 18 C 18 C C 18 ph stability 1 9 (CH3) 3 (CH3) 3 zwitterionic ammonium sulfonic acid modification 7 % C ph stability 2 8.5, N S 3 + N S 3 + cyano (nitrile) phase for NP and RP separations 7 % C USP L10 ph stability 1 8, stable towards highly aqueous mobile phases C N C N (CH3) 3 amino phase for NP and RP separations 2.5 % C USP L8 ph stability 1 8, stable towards highly aqueous mobile phases NH 2 NH 2 unmodified high purity silica USP L3 n.a. ph stability 2 8 * = hydrophobic selectivity, = polar/ionic selectivity, C = steric selectivity 188 Toll Free: info@lifescience.ca Web:
3 verview of NUCLEDUR Phases NUCLEDUR (Macherey-Nagel) pplication milar phases** Separation principle Retention mechanism in general compounds with ionizable functional groups such as basic pharmaceuticals and pesticides for Gravity generally shorter retention times for nonpolar compounds NUCLESIL C 18 HD Waters Xterra RP 18 / MS C 18 ; Phenomenex Luna C18 (2), Synergi and Max RP; Zorbax Extend C18; Purospher RP-18; Star RP-18 NUCLESIL HD; Waters Xterra RP 8 / MS ; Phenomenex Luna C8; Zorbax Eclipse; XD-C8 only hydrophobic (van der Waals ) (CH3)3 H3C CH3 N high steric selectivity, thus suited for separation of positional and structural isomers, planar/nonplanar molecules NUCLESIL C 18 YMC Pro C18RS steric and hydrophobic basic pharmaceutical ingredients, very polar compounds, organic acids Phenomenex qua; YMC Q; Waters tlantis dc18 hydrophobic CH3 and polar N H3C (H bonds) compounds with aromatic and multiple bond systems no similar phases π-π and hydrophobic N2 robust C 18 / phase for routine analyses hydrophilic compounds such as organic polar acids and bases, polar natural compounds NUCLESIL C 18 Spherisorb DS II; Hypersil DS; Waters Symmetry C18; Kromasil C18 LiChrospher RP 18 NUCLESIL ec / Spherisorb C8; Hypersil MS; Waters Symmetry C8; Kromasil C8; LiChrospher RP 8 Merck Sequant ZIC -HILIC; elc belisc only hydrophobic (van der Waals ) some residual silanol ionic / hydrophilic, electrostatic CH3 (CH3)3 N H3C H 3C N + S 3 CH3 H N NH H 3C NH 2 N + S 3 polar organic compounds (basic drugs), molecules containing π electron systems NUCLESIL CN / CN-RP π-π, polar (H bonds), hydrophobic C N C N H sugars, sugar alcohols and other hydroxy compounds, DN bases, polar compounds in general NUCLESIL NH 2 / NH 2 -RP polar /ionic, hydrophobic + NH3 polar compounds in general unmodified NUCLESIL polar /ionic 2N ** phases which provide a similar selectivity based on chemical and physical properties Toll Free: info@lifescience.ca Web: 189
4 NUCLEDUR (Macherey-Nagel) NUCLEDUR HILIC Ideal for reproducible and stable chromatography of highly polar analytes Suitable for analytical and preparative applications as well as LC-MS Very short column conditioning period NUCLEDUR HILIC is a special zwitterionic modified stationary phase based on ultra spherical NUCLEDUR particles. The betaine character of the ammonium-sulfonic acid ligands results in total charge equalization and in an overall neutrally charged but highly polar surface. Retention characteristics Commonly HILIC is described as partition chromatography or liquid/liquid extraction system between the mobile and stationary phase. water-poor layer of mobile phase is formed on the surface of the polar stationary phase versus a water-rich layer. Therefore, a distribution of the analytes between these two layers will occur. HILIC includes weak electrostatic mechanisms as well as hydrogen donor between neutral polar molecules under high organic elution conditions. This distinguishes HILIC from ion exchange chromatography. More polar compounds will have stronger interaction with the stationary aqueous layer than less polar compounds resulting in a stronger retention. Non-polar compounds exhibit faster elution profiles due to minor hydrophobic. s seen in Figure 1, the elution order is quite often inverse on HILIC columns compared to RP columns. Figure 1. Separation of uracil and naphthalene Figure 2. Stability and equilibration Fields of application Due to its high loadability, NUCLEDUR HILIC is suitable for preparative and semi-preparative applications. ased on their special interaction and retention features, HILIC columns are ideal for analysis of medium-polar and polar compounds. Determinations of polar compounds in foods, such as melamine in milk or acrylamide in bakery products, are as necessary as separations of vitamins and organic acids in food and pharmaceutical products. Stable and effective application notes were developed on NUCLEDUR HILIC for pharmaceutical and physiological substances (ie. the chemotherapeutic agent 5-fluorouracil, the energy carrier of muscle metabolism creatine), catecholamines and amino acids. In bioanalysis, nucleotides as well as pyrimidine and purine bases can be determined efficiently. ecause well-biodegradable and polar pesticides have to be analyzed, the determination of chlormequat, mepiquat, paraquat and diquat gains increasing importance (see Figure 3). Figure 3. Separation of growth regulators Stability features Due to an advanced and unique surface modification procedure (pat. pend.) NUCLEDUR HILIC columns provide short equilibration times (see Figure 2). fter just 5 min. equilibration the 2 nd injection shows stable and reproducible results. NUCLEDUR HILIC columns are characterized by an outstanding column lifetime. fter nearly 800 runs, the columns show no loss of pristine performance. Peak shape and retention are still immaculate. Determinations of hydrocarbons, peptides and glycolized or phosphorylized compounds are other successful HILIC applications. 190 Toll Free: info@lifescience.ca Web:
5 NUCLEDUR (Macherey-Nagel) NUCLEDUR 1.8µm Phases Ultra fast LC Shorter columns with high separation efficiency gnificant improvement in resolution Increased detection sensitivity Suitable for LC-MS due to low bleeding characteristics ll NUCLEDUR premium phases are now available in 1.8µm particle sizes. Columns packed with these microspherical particles show improvement in terms of plate numbers, column efficiencies and resolution compared with their 3µm counterparts. Increase in separation efficiency n increase in plate number of approximately 67% in transferring from 3µm to 1.8µm particles offers the possibility of using shorter columns with equal plate numbers resulting in a decrease in analysis time. 50mm x 4.6mm NUCLEDUR C18 Gravity 3µm: N 100,000 plates/m (h value 10) 1.8µm: N 166,667 plates/m (h value 6) Improvement in resolution The use of 1.8µm instead of 3µm particles can lead to an increase in resolution of approximately 30%. This is illustrated in Figure 1 for the separation of naphthalene and ethylbenzene. Column back pressure The smaller particle size results in an increased back pressure. However, the high sphericity of the NUCLEDUR particles and their very narrow particle size distribution means that back pressure is kept at a moderate level. In addition, the particles are fractionated in order to limit the increase in back pressure. Nevertheless, the use of columns packed with sub 2µm particles generally makes special demands on the LC equipment. Pumps should be designed for pressures of psi and the entire system should feature the lowest possible dead volume for optimum column performance. Higher flow rates and shorter run times The optimal flow rate for 1.8µm particles is higher than for 3 and 5µm particles. Figure 2 shows the considerable decrease in analysis time on converting from a 5µm to a 1.8µm column and increasing the flow rate. See pg. 195 for all NUCLEDUR part numbers and pricing Figure 1. Resolution as a function of particle size Macherey-Nagel literature available, please enquire Figure 2. Reduction of analysis time Toll Free: info@lifescience.ca Web: 191
6 NUCLEDUR (Macherey-Nagel) NUCLEDUR PolarTec NUCLEDUR PolarTec shows outstanding stability in highly aqueous media (>95%) due to a covalently bonded C18 silane with an embedded polar functional group. In addition to the hydrophobic between analyte and stationary phase shown by standard C18 RP phases, Nucleodur PolarTec also shows polar interaction via hydrogen bonding and dipole-dipole forces. This can have an influence on retention time and improve selectivity, particularly for the separation of polar compounds. Figure 1 shows the separation of organic acids with Nucleodur PolarTec compared with a standard RP18 phase. Figure 1. rganic acids comparison Eluents: : cetonitrile +0.1%TF Sample : Water +0.1%TF 1. Dihydroxymandelic cid 6. Vanillic cid Gradient: 20% > 60% in 12 min 2. Gallic cid 7. 3-Hydroxybenzoic cid Flow: 0.73 ml/min 3. Dihydroxyphenylacetic cid Dihydroxybenzoic cid Temperature: 20 C 4. 3,4-Dihydroxybenzoic cid Dihydroxybenzoic cid Detection: 254nM 5. Syringic cid NUCLEDUR PolarTec 5µm, EC 150/3 RP18 5µm, EC 150/3 0,0 1,0 2,0 3,0 4,0 5,0 7,0 8,0 9,0 10,0 0,0 1,0 2,0 3,0 4,0 5,0 7,0 8,0 9,0 10,0 NUCLEDUR PFP The functional group of Nucleodur PFP is a covalently bonded, Pentafluorophenyl propyl ligand that allows the separation of small, water soluble molecules, as well as peptides, polar or basic compounds and positional isomers. Nucleodur PFP shows a variety of between the analyte and the solid phase. esides the hydrophobic and dipole-dipole, the perfluorinated aromatic ring system (which is electron poor due to I effect of the fluor atoms), additional p-p and hydrogen bonding allow the base line separation of positional isomeric dichlorophenols. Figure 2. 80,0 40,0 20,0 0,0 2,0 4,0 8,0 10,0 12,0 14,0 Column: Eluents: Flow: Temperature: 35 C Detection: 280nM Sample 1. o-cresol 2. m-cresol 3. 3,4-Dimethylphenol 4. 3,5-Dimethylphenol 5. 2,5-Dimethylphenol 6. 2,6-Dichlorophenol 7. 2,2-Dichlorophenol 8. 2,4-Dichlorophenol 9. 3,4-Dichlorophenol 10. 2,4-Dibromophenol 11. 3,5-Dichlorophenol NUCLEDUR PFP 5µm, 125mm x 4mm : cetonitrile +0.1%F/ Water +0.1%F (35:65, v/v) 1 ml/min See pg. 195 for part numbers and pricing 192 Toll Free: info@lifescience.ca Web:
Overview Applications of NUCLEODUR HPLC Phases
verview Applications of HPLC Phases Phase Specification Characteristics* Stability Structure A B C octadecyl phase, high density coating multi-endcapping 8% C USP L ph stability -, suitable for LC/MS (Si-
More informationPackings for HPLC. Packings for HPLC
Summary of packings for HPLC In analytical HPLC, packings with particle sizes of 3 to 10 µm are preferred. For preparative separation tasks, also particles with diameters larger than 10 µm are applied.
More informationMethod Development Kits
Method Development Kits sales representative for ordering information or contact...77 Method Development Kits First column choice for Pharma Development USP L C Method Development Kits Maximize retention
More informationInertsil ODS-EP Technical Information
Technical Information Advantages of The selectivity is completely different from those of conventional columns such as ODS column due to its specific polar group in the stationary phase. Durability can
More informationACE SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE SuperC8 TM Ultra-Inert UHPLC and HPLC s with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS compatible buffers between ph.. High efficiency µm, µm, µm and
More informationColumns and supplies catalog
Columns and supplies catalog PLC GC TLC SPE and lash Syringe filters Vials and caps... we Meet your Needs MACEREY-NAGEL Company profile Since 1911 MACEREY-NAGEL has represented high quality, innovation
More informationProduct Bulletin. ACE LC/MS and Rapid Analysis HPLC Columns. HPLC Columns
Product Bulletin HPLC Columns ACE LC/MS and Rapid Analysis HPLC Columns 0 mm, 30 mm, 35 mm and 50 mm column lengths.0,., 3.0, 4.0 and 4.6 mm column diameters Configured for High Sample Throughput Specially
More informationState-of-the-art C18 HPLC Columns
An HPLC GL Sciences Newest and Most Advanced ODS Phase-New For 00 State-of-the-art C HPLC s Improved Peak Shapes and Heights Enhancing Sensitivity High Resolution Fast Equilibration Compatible with 00%
More information( )( ) Selectivity Choices in Reversed-Phase Fast LC. Introduction. R s = 1 a 1 k 4 a 1 + k
Selectivity Choices in Reversed-Phase Fast LC Luisa Pereira, Monica Dolci, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20543 Key Words Accucore, Hypersil GOLD, Syncronis, Column Chemistry,
More informationOptimisil HPLC & UPLC Column catalogue
Optimisil HPLC & UPLC Column catalogue Analytical Hub Plot No: 37, P & T Colony, Vikrampuri, Secunderabad 500009 Tel: 040-64621313, Mobile: +91 7799931313 Website: www.analyticalhub.com email: contact@analyticalhub.com
More informationNUCLEOSHELL core-shell silica for HPLC
NUCLESHELL core-shell silica for HPLC Core-shell technology µm 0. µm.8 µm 0. µm.7 µm 0. µm.7 µm 0. µm Key feature Solid core of silicon dioxide, homogeneous shell of porous silica Highest efficiency compared
More informationpenta-hilic UHPLC COLUMNS
penta-hilic UHPLC COLUMNS penta-hilic Highly retentive, proprietary penta-hydroxy-ligand Excellent peak shape for polar compounds with a variety of functional groups: acids, bases, zwitterions strong and
More informationSample Preparation TLC Plates
TLC PLATES TLC Plates Economical separation method High sample throughput Pilot procedure for HPLC and flash chromatography Versatile range of ready-to-use layers Thin layer chromatography (TLC) is a simple,
More informationComparison Guide to C18 Reversed Phase HPLC Columns
Comparison Guide to C18 Reversed Phase HPLC Columns Comparison Data on Commonly Used C18 Phases Stationary Phase Specifications Phases Compared According to Relative Hydrophobicity Phases Compared According
More informationOrosil HPLC Columns. OroSil HPLC columns are designed for the separation of polar, semi-polar, and nonpolar compounds at low to medium ph.
Orosil OroSil HPLC columns are designed for the separation of polar, semi-polar, and nonpolar compounds at low to medium ph. Excellent organic base selectivity with very low asymmetry values Compatible
More informationHypersil BDS Columns TG 01-05
TG 0-0 Hypersil BDS Columns Introduction Hypersil BDS columns have gained a reputation over the years as one of the most robust, reproducible and reliable HPLC column brands available. This Technical Guide
More informationChemistry Instrumental Analysis Lecture 28. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 28 High Performance Liquid Chromatography () Instrumentation Normal Phase Chromatography Normal Phase - a polar stationary phase with a less polar mobile phase.
More informationInertSustainSwift C8
Physical Properties Silica Particle Size Surface Area Pore Size Pore Volume Bonded Phase End-capping Carbon Loading ph Range USP Code :ES (Evolved Surface) Silica Gel :.9 μm, μm, μm :00 m /g :00 Å (0 nm)
More informationLC and LC/MS Column Selection Flow Chart
LC and LC/MS Column Selection Flow Chart To use the column selection diagram below, simply follow the path for your analyte and mobile phase. At the far right, follow your final column selection to the
More informationpenta-hilic UHPLC COLUMNS
penta-hilic UHPLC COLUMNS Highly retentive, proprietary penta-hydroxy-ligand penta-hilic Excellent peak shape for polar compounds with a variety of functional groups: acids, bases, zwitterions strong and
More informationLIQUID CHROMATOGRAPHY
LIQUID CHROMATOGRAPHY RECENT TECHNIQUES HPLC High Performance Liquid Chromatography RRLC Rapid Resolution Liquid Chromatography UPLC Ultra Performance Liquid Chromatography UHPLC Ultra High Pressure Liquid
More informationACE Excel SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE Excel SuperC8 TM Ultra-Inert UHPLC and HPLC s with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS-compatible buffers between ph.. High efficiency µm, µm,
More informationHydrophilic Interaction Liquid Chromatography: Some Aspects of Solvent and Column Selectivity
Hydrophilic Interaction Liquid Chromatography: Some Aspects of Solvent and Column Selectivity Monica Dolci, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20544 Key Words Hydrophilic, HILIC,
More informationAn HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint
An HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint An important new column in the Inertsil series Bonded-Phase Structure Perfect balance of retention for both polar
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS SeQuant ZIC-HILIC. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS SeQuant ZIC-HILIC Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 PE, UK Tel: + (0)8 90 660 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationACE Ultra Inert Base Deactivated HPLC Columns
ACE Ultra Inert Base Deactivated HPLC Columns Contents Page ACE HPLC Columns...- Independent Comparison of HPLC Columns #... Independent Comparison of HPLC Columns #... ACE 00Å HPLC Columns Specifications...-
More informationzorbax Packed by Phenomenex Material Characteristics High efficiency Guaranteed quality Phenomenex columns packed with Zorbax
Packed by Phenomenex High efficiency Guaranteed quality Phenomenex columns packed with 7 μm materials offer the advantages of lower cost as well as lower operating backpressures, which result in long column
More informationLC Columns with Liquid Separation Cell Technology
Obelisc LC Columns with Liquid Separation Cell Technology Obelisc HPLC Columns - Liquid Separation Cell Technology Introduction Obelisc HPLC columns are the latest, innovative columns from SIELC Technologies,
More informationCOMPARISON GUIDE. to C18 Reversed Phase HPLC Columns. Comparison Data on 60 Commonly Used C18 Phases. Stationary Phase Specifications
COMPARISON GUIDE to C18 Reversed Phase HPLC Columns Comparison Data on 60 Commonly Used C18 Phases Stationary Phase Specifications Phases Compared According to Relative Hydrophobicity Phases Compared According
More informationRP C18 column with feature of a silanol group
RP C8 column with feature of a silanol group lanol Activity Controlled C8 Column Sunrise ctacosyl C8) Sunrise ctadecyl C8) Sunrise ctadecyl-sac C8-SAC has an interaction of silanol groups ChromaNik Sunrise
More informationHPLC Column Selection: Solve the Separation Mystery. The world leader in serving science
HPLC Column Selection: Solve the Separation Mystery The world leader in serving science verview Introduction Historical verview in Stationary Phases Basics in Column Selection Resolution, Efficiency and
More informationFluophase and Fluofix Columns
TG 0-0 Fluophase and Columns Introduction Fluorinated packings exhibit extra retention and selectivity for compounds that have fluorine and chlorine substituents. They also exhibit shape selectivity for
More informationCHROMATOGRAPHY. NUCLEOSIL the original HPLC. Find what you are looking for!
CHROMATOGRAPHY HPLC NUCLEOSIL the original Find what you are looking for! www.mn-net.com MACHEREY-NAGEL Innovation based on tradition Our company For more than 100 years, MACHEREY-NAGEL has provided highest
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS SIELC Primesep. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS SIELC Primesep Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)8 90 660 Fax: +44 (0)8 9 484 Email: sales@hichrom.co.uk
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Zorbax. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Zorbax Catalogue 9 1 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484 Email:
More informationToo Polar for Reversed Phase What Do You Do?
Too Polar for Reversed Phase What Do You Do? June 20, 2013 Mark Powell Columns and Consumables Technical Support Page 1 C8 or C18 Doesn t Always Do the Job Typical reversed phase conditions involve water/buffer
More informationInertSustainSwift C8
HPLC, LC/MS Columns InertSustainSwift TM C8 New! InertSustainSwift C8 is an octyl group (C8) bonded column offering the same extreme inertness to any type of compounds just like InertSustainSwift C8, which
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS HALO and HALO-5. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS HALO and HALO- Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG PE, UK Tel: + (0)8 90 0 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development
ACE C8-Amide For increased polar retention and alternative selectivity Alternative selectivity for method development Improved separations with polar, acidic, basic and phenolic compounds High efficiency
More informationInertsil Technical Library
HPLC COLUMN INERTSIL TECHNICAL LIBRARY H P L C C o l u m n Inertsil Technical Library Inertsil ODS-4, C8-4 Comparison of Performance List of Compared Columns Experimental Explanation, Analytical Conditions
More informationEpic Aromatic Selectivity
Epic Aromatic Selectivity HPLC Columns p h a r m a c e u t i c a l e n v i r o n m e n t a l c h e m i c a l b i o c h e m i c a l s e p a r a t i o n & p u r i f i c a t i o n ES Industries 701 S. Route
More informationYour Reversed Phase Method
PLC Columns for Your Reversed Phase Method Balanced Non-Polar & Polar Selectivity Strong Non-Polar & Polar Selectivity Strong Polar & Aromatic Selectivity Unique Isomeric & alogenated Selectivity Robust
More information1.7µm Fortis UHPLC Columns
Strength in Technology.7µm Fortis Columns NEW Phase Chemistries - Fortis Ho (polar endcapped) - Fortis Amino - Fortis HILIC Diol Ultra High Pressure Chromatography 8 Chemistry Choices Increase Efficiency
More informationLC Technical Information
LC Technical Information Method Transfer to Accucore.6 μm Columns Containing solid core particles, which are engineered to a diameter of.6μm and a very narrow particle size distribution; Accucore HPLC
More informationKromasil. for your analytical HPLC. Kromasil. The way to peak performance in liquid chromatography
Kromasil for your analytical HPLC Kromasil The way to peak performance in liquid chromatography Kromasil is known, worldwide, for its high performance and excellent total economy in preparative and industrial
More informationThere s Great Chemistry Between Us.
There s Great Chemistry Between Us. Introduction HPLC Columns Theory, Technology and Practice Uwe D. Neue, Waters Corporation, Milford, MA published by John Wiley & Sons High performance liquid chromatography
More informationPart 2. Overview of HPLC Media
Part 1. General Chromatographic Theory Part 2. Overview of HPLC Media Part 3. The Role of the Mobile Phase in Selectivity Part 4. Column Care and Use 1 HPLC Particle Technology Core-Shell Particle Fully
More informationAnalysis - HPLC A.136. Primesep 5 µm columns B.136
Primesep 5 µm columns Primesep columns feature double functionality of the bonding i.e : alkyl chain with anionic or cationic group, chelating group. This feature creates unique selectivities when using
More informationAlltech Alltima HP Introduction
Alltech Introduction High-Stability, High-Purity, High-Performance, Low-Bleed Columns for Demanding Applications Better Peak Symmetry high-purity silica eliminates peak tailing problems Long Column Life
More informationNovel LC/MS-Compatible Stationary Phase with Polar Selectivity
Novel LC/MS-Compatible Stationary Phase with Polar Selectivity Carmen T. Santasania and David S. Bell Supelco 595 N. Harrison Rd., Bellefonte, PA 16823 T403168 GJU Introduction Stationary phases based
More informationThe Evolving HPLC Column Packings
The Evolving HPLC Column Packings GL Sciences has been steadily supplying with columns from Inertsil ODS, first-generation to InertSustain series, integration of state-ofthe-art technologies, and has established
More informationSTUDY OF LOADABILITY AND SELECTIVITY OF PHARMACEUTICAL COMPOUNDS ON RPLC COLUMNS
STUDY OF LOADABILITY AND SELECTIVITY OF PHARMACEUTICAL COMPOUNDS ON RPLC COLUMNS Fang Xia, Jie Y. Cavanaugh, Uwe Neue, Jeffrey R. Mazzeo, Diane M. Diehl Waters Corporation, Milford, MA USA OVERVIEW- INTRODUCTION-
More informationCapital HPLC Columns and HPLC Cartridges. Optimal HPLC Range
Capital HPLC Columns and HPLC Cartridges Specialty Columns HPLC Columns & HPLC Cartridges Download Capital Catalog Adsorbosphere Apex Chromegabond Diasogel Exsil Hypersil Inertsil ODS-2 Partisil Rosil
More informationACE C18-PFP. Hydrophobic and pentafluorophenyl mixed mode interaction. High efficiency 2µm, 3µm, 5µm and 10µm particles for UHPLC and HPLC
ACE C-PFP A C bonded phase with unique selectivity F F F F F Guaranteed reproducibility Exceptional bonded phase stability Hydrophobic and pentafluorophenyl mixed mode interaction High efficiency µm, µm,
More informationThermo Scientific Syncronis HPLC Columns. Remarkable separations guaranteed time after time
Thermo Scientific Syncronis HPLC Columns Remarkable separations guaranteed time after time Syncronis HPLC and UHPLC Columns Remarkable separations guaranteed time after time When developing a new method,
More informationComparison of different aqueous mobile phase HPLC techniques
June 009 ewsletter Pharmaceutical Analysis: What is Your Problem? SIELC Technologies, Inc., Prospect Heights, IL 0070 Pharmaceutical analysis involves liquid chromatography of various compounds, from active
More informationN α - 1 k. R s = Figure 1: The effect of N, α and k on resolution (Rs)
A new high performance reversed phase column that leverages the advantages of a C phase with the extra selectivity of a pentafluorophenyl (PP) phase. columns separate mixtures not possible with ordinary
More informationProntoSIL C18-EPS Reversed-Phase HPLC Columns
ProntoSIL C8-EPS Reversed-Phase HPLC Columns Provides excellent separation of polar compounds Better peak shape for acids and bases Stabilized bonded phase for rugged, robust HPLC methods More retentive
More informationTracer Excel TRACER EXCEL ODS-A. Total deactivation. Maximum Stability
Tracer Excel TRACER EXCEL is a range of totally new packings that employ the most advanced procedures of synthesis and chemical functionalization, resulting in some column packings that completely surpass
More informationACE SuperC18. ACE UHPLC and HPLC Columns. Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability
ACE SuperC8 TM Ultra-Inert UHPLC and HPLC Columns with Extended ph Stability Exploit selectivity at low, intermediate and high ph Use with LC/MS compatible buffers between ph.. High efficiency µm, µm,
More informationSPE Columns. Solid Phase Extraction. SPE Columns
SPE Columns Solid Phase Extraction Solid-phase extraction (SPE) is a separation process by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the
More informationSunniest C18 Sunniest RP-AQUA Sunniest C8 Patent pending
Patent pending Website : www.chromtech.net.au E-mail : info@chromtech.net.au telno : 0 97 0... in AUSTRALIA A Novel Bonding Technique (patent pending) An unique trifunctional silyl-reagent was developed
More informationUptisphere CS Evolution Core Shell columns for fast & highly efficient identification & quantification of small molecules
Uptisphere CS Evolution Core Shell columns for fast & highly efficient identification & quantification of small molecules www.interchim.com www.blog.interchim.com www.forum.interchim.com www.facebook.com/interchim
More informationNew 5-micron HALO-5 columns based on Fused- Core particle technology boost the performance of HPLC. Compared to other HPLC columns, HALO-5 columns
New 5-micron HALO-5 columns based on Fused- Core particle technology boost the performance of HPLC. Compared to other HPLC columns, HALO-5 columns have: the highest plate number versus any other 5-micron
More informationPacked Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS. Table of Contents
No. 089 SEPARATION REPORT Packed Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS Table of Contents 1. Introduction 1 2. Column Specification 1 3. Features of Packing Materials
More informationTechnical Guide Thermo Scientific Syncronis HPLC Columns
Technical Guide Thermo Scientific Syncronis HPLC Columns Consistent, predictable separations, Column after column, time after time Thermo Scientific Syncronis Columns When developing a new method, one
More informationCourse goals: Course goals: Lecture 1 A brief introduction to chromatography. AM Quality parameters and optimization in Chromatography
Emqal module: M0925 - Quality parameters and optimization in is a separation technique used for quantification of mixtures of analytes Svein.mjos@kj.uib.no Exercises and lectures can be found at www.chrombox.org/emq
More informationNew Product Update. HPLC Columns
New Product Update HPLC Columns ZORBAX MicroBore HPLC Columns ZORBAX Extend-C18 Columns ZORBAX Bonus-RP Columns Inertsil ODS-2 Cartridge Columns ZORBAX Carbohydrate Analysis Columns ZORBAX Eclipse dsdna
More informationCation Exchange HPLC Columns
Cation Exchange HPLC Columns Hamilton offers seven polymeric packing materials for cation exchange separations. Type Recommended Application(s) PRP-X00 PRP-X00 PRP-X800 HC-0 HC-7 Ca + HC-7 H + HC-7 Pb
More informationReversed Phase Solvents
Part 1. General Chromatographic Theory Part 2. verview of HPLC Media Part 3. The Role of the Mobile Phase in Selectivity Part 4. Column Care and Use Reversed Phase Solvents 2 Solvents for RP Chromatography
More information8. Methods in Developing Mobile Phase Condition for C18 Column
I. HPLC Columns Technical Information 8. Methods in Developing Mobile Phase Condition for C18 Column Introduction In reversed phase HPLC, octadecyl group bonded silica columns (C18, ODS) are the most widely
More informationChapter content. Reference
Chapter 7 HPLC Instrumental Analysis Rezaul Karim Environmental Science and Technology Jessore University of Science and Technology Chapter content Liquid Chromatography (LC); Scope; Principles Instrumentation;
More informationQUALITY HPLC COLUMNS PARTICLE PORE PAGE SIZE SIZE. C18 Bidentate C18 Extremely long column life and rugged 4um 100Å 3
Cogent HPLC Columns Manufactured by Table of Contents QUALITY HPLC COLUMNS PARTICLE PORE PAGE SIZE SIZE C18 Bidentate C18 Extremely long column life and rugged 4um 100Å 3 C8 Bidentate C8 Extremely long
More informationChoosing Columns and Conditions for the Best Peak Shape
Choosing Columns and Conditions for the Best Peak Shape What is Good Peak Shape and Why is it Important? Good peak shape can be defined as a symmetrical or gaussian peak and poor peak shape can include
More informationSimultaneous Determination of Paraquat and Diquat in Environmental Water Samples by HPLC-MS/MS
Simultaneous Determination of Paraquat and Diquat in Environmental Water Samples by HPLC-MS/MS Richard Jack, Xiaodong Liu, Leo Wang, and Chris Pohl OT70806_E 08/13S 1 The world leader in serving science
More informationHPLC. Sorbtech Purity HPLC Columns. Macherey-Nagel HPLC Columns NUCLEODUR NUCLEOSHELL NUCLEOSIL. Mitsubishi MCI Gel HPLC Columns
Sorbtech offers all types of metal jacketed columns made in-house with the highest quality materials available as well as columns manufactured by Macherey-Nagel, MCI Gel, and Jordi Labs. Sorbtech Purity
More informationHPLC Column Material - Bulk Ware
HPLC Column Material - Bulk Ware Constantly growing demands on separation efficiency and availability of solid phase material for packing HPLC columns and column packing stations have motivated us to provide
More informationImpSilHPLC Columns. The solution for your chromatography IMPTECH SCIENTIFIC
ImpSilHPLC Columns The solution for your chromatography IMPTECH SCIENTIFIC 7-1-60,203-Veena Dharam Karam Road,Ameerpet,Hyderabad-500 016 Tel:040-23740443/483 Ph:9848051071 imptechscientific@gmail.com www.impscience.com
More informationKromasil Eternity Designed for long life. For use in HPLC and UHPLC
Kromasil Eternity Designed for long life For use in HPLC and UHPLC The long-lasting phase for demanding applications Imagine working under virtually no ph restrictions during method development. That s
More informationfor Acclaim Mixed-Mode HILIC-1 Column
for Acclaim Mixed-Mode HILIC-1 Column Product Manual for ACCLAIM Mixed-Mode HILIC-1 Page 1 of 17 Product Manual for ACCLAIM Mixed-Mode HILIC-1 Column 5µm, 4.6 x 250mm, P/N 066844 5µm, 4.6 x 150mm, P/N
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Vydac. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Vydac Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG PE, UK Tel: + (0)8 90 660 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk
More informationZirconia-Based Phases as a Powerful Complement to Silica-Based Phases for LC and LC-MS under Non-extreme Mobile Phase Conditions
Zirconia-Based Phases as a Powerful Complement to Silica-Based Phases for LC and LC-MS under on-extreme Mobile Phase Conditions Richard A. Henry, Shawn R. Wyatt, Carmen T. Santasania and David S. Bell
More informationIt s hot! ZIC -chilic
It s hot! ZIC -chilic Complementary selectivity for HPLC and LC-MS separation of polar hydrophilic compounds EMD Millipore Corp. is a subsidiary of Merck KGaA, Darmstadt, Germany ZIC -chilic Your benefits
More informationHigh Performance Liquid Chromatography
High Performance Liquid Chromatography What is HPLC? It is a separation technique that involves: Injection of small volume of liquid sample Into a tube packed with a tiny particles (stationary phase).
More informationSEPARATIONS ESSENTIALS IN MODERN HPLC. 2University of Bucharest, Bucharest, Romania
ESSENTIALS IN MODERN HPLC SEPARATIONS Serban C. Moldoveanu1, Victor David2 'R.J. Reynolds Tobacco Co., Winston-Salem, NC, USA 2University of Bucharest, Bucharest, Romania ELSEVIER AMSTERDAM BOSTON HEIDELBERG
More informationHICHROM. Chromatography Columns and Supplies. LC COLUMNS Thermo Scientific Acclaim. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies LC COLUMNS Thermo Scientific Acclaim Catalogue The Markham Centre, Station Road Theale, Reading, Berks, RG PE, UK Tel: + (0) 0 0 Fax: + (0) Email: sales@hichrom.co.uk
More informationTransferring Yesterdays Methods to Tomorrows Technology: The Evolution from HPLC to UPLC TM
Transferring Yesterdays Methods to Tomorrows Technology: The Evolution from HPLC to UPLC TM Eric S. Grumbach Thomas E. Wheat Chuck Phoebe Joe Arsenault Jeffrey R. Mazzeo Diane M. Diehl UPLC TECHNOLOGY
More informationKromasil Eternity Designed for long life
Kromasil Eternity Designed for long life For use in HPLC and UHPLC Kromasil The long-lasting phase for demanding applications Imagine working under virtually no ph restrictions during method development.
More informationOpen Column Chromatography, GC, TLC, and HPLC
Open Column Chromatography, GC, TLC, and HPLC Murphy, B. (2017). Introduction to Chromatography: Lecture 1. Lecture presented at PHAR 423 Lecture in UIC College of Pharmacy, Chicago. USES OF CHROMATOGRAPHY
More informationThermo Scientific Accucore XL HPLC Columns. Technical Manual
Thermo Scientific Accucore XL HPLC Columns Technical Manual Thermo Scientific Accucore XL HPLC Columns Based on Core Enhanced Technology using µm solid core particles, Accucore XL HPLC columns allow users
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 1 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationHPLC Columns. HPLC Accessories HPLC Column Test Mixes Reference Books...www.restek.com. HPLC Applications...
HPLC Columns HPLC Column Selection................................292-297 UHPLC Columns........................................298-299 Capillary LC Columns........................................300 Pinnacle
More informationFast Separation of Vastly Different Compounds by Isocratic HPLC
Fast Separation of Vastly Different Compounds by Isocratic HPLC Aimee N. Heyrman and Yury Zelechonok Resolution Systems, Inc. 590 E. 32nd St. Holland. MI, 49423 SIELC Technologies, 15 E. Palatine Rd, Suite
More information1.7 m Fortis UHPLC Columns
Strength in Technology 1.7 m Fortis Columns Ultra High Pressure Chromatography Improve your Performance 8 Chemistry Choices Increase Efficiency Increase Speed Improve Resolution Greater Sensitivity Lower
More informationcolumns Acclaim Mixed-Mode WCX-1 for Separating Basic Molecules
columns Acclaim Mixed-Mode WCX- for Separating Basic Molecules The Acclaim Mixed-Mode WCX- is a novel, high-efficiency, silica-based column specially designed for separating various basic analytes. This
More informationWilliam E. Barber, Ph.D. Applications Chemist October
William E. Barber, Ph.D. Applications Chemist ctober 0 00 Secrets of Good Peak Shape in HPLC Time: :00 p.m. CET Telephone Number: + 44 0 76 088 Chairperson: John Vis The Secrets of Good Peak Shape in HPLC
More informationHow proteins separate on reverse-phase HPLC
1 Reverse Phase How proteins separate on reverse-phase HPLC RP chromatography separates proteins through the interaction of the hydrophobic foot of the protein with a nonpolar surface of the particle RP
More informationAdvantages of polymerbased. an alternative for ODS
Advantages of polymerbased HPLC columns an alternative for ODS Yukiko Higai Shodex, 1 Content (1) Comparison between Silica and Polymer-based RP columns (2) Characteristics of Polymer RP columns - an example:
More informationHPLC Products. New 1.9μm Pinnacle DB Small Particle HPLC Columns. 2007/08 Edition. Chromatography Products. hplc columns.
HPLC Products 2007/08 Edition hplc columns hplc accessories hplc replacement parts analytical reference materials vials & syringes New.9μm Pinnacle DB Small Particle HPLC Columns See page 8 for details.
More information