Your Reversed Phase Method

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1 PLC Columns for Your Reversed Phase Method Balanced Non-Polar & Polar Selectivity Strong Non-Polar & Polar Selectivity Strong Polar & Aromatic Selectivity Unique Isomeric & alogenated Selectivity Robust C8 Selectivity

2 PFP() Choose the Optimal PLC Column for Your Application Choosing the right column can ease method development and result in improved resolution.* Analytes Polar Non Polar Polar/Non-Polar Mixtures Recommended PLC column Synergi Polar-RP Gemini -NX Synergi ydro-rp Stronger non-polar retention Synergi Fusion-RP Balanced non-polar & polar retention For Alternative Selectivity Luna PFP() Selectivity for isomeric, aromatic, and halogenated compounds OR Luna ILIC** For very polar compounds consider ILIC mode Luna PFP() More aromatic selectivity Synergi ydro-rp More hydrophobic selectivity *These are suggestions and individual results may vary, depending on your sample and separation goal. **Contact us for more information on Luna ILIC columns or visit Trademarks Luna and Gemini are registered trademarks of Phenomenex, Inc. SecurityGuard and Synergi are trademarks of Phenomenex, Inc. Waters is a registered trademark, and SymmetryShield is a trademark of Waters Corp. Disclaimer Comparative separations may not be representative of all applications. Subject to Phenomenex Standard Terms & Conditions, which may be viewed at Phenomenex, Inc. All rights reserved.

3 Gemini -NX C8 Robust C8 Performance at p Stable - Mobile phase p directly impacts retention and resolution of most drug compounds. Using Gemini-NX, method development is nearly unlimited due to the advanced column technology that provides wider p stability and longer column lifetime. By using mobile phase p to change the ionization state of your compound, you can increase retention of your compounds. Ionized state Nicotine spends less time interacting with the C8 stationary phase and elutes in the void volume App ID 666 p.8 Charged Analyte O N O - N + O Nicotine in 0. % Formic Acid, p.8 Column: Gemini-NX 5 µm C8 Part No.: 00F-5-E0 Mobile Phase: A: 0. % Formic Acid in Water B: 0. % Formic Acid in Acetonitrile Gradient: A/B (95:5) to (5:95) in 0 min, old for min Flow Rate:.5 ml/min Temperature: Ambient Detection: 5 nm Sample: Nicotine; pk a min Neutral state Nicotine has distinct chromatographic behaviors: Increased retention - nicotine spends more time interacting with the stationary phase Sharp peak - single neutral form, well resolved App ID 667 p 0.5 Uncharged Analyte O - O - O - N O - N O O - Nicotine in Ammonium Bicarbonate, p 0.5 Column: Gemini-NX 5 µm C8 Part No.: 00F-5-E0 Mobile Phase: A: 0 mm Ammonium Bicarbonate p 0.5 B: Acetonitrile Gradient: A/B (95:5) to (5:95) in 0 min, old for min Flow Rate:.5 ml/min Temperature: Ambient Detection: 5 nm Sample: Nicotine ; pk a min 3

4 Synergi YDRO-RP Strong Non-polar Selectivity with Polar Retention For sample mixtures that require separation of non-polar and polar analytes, Synergi ydro-rp exhibits very hydrophobic retention compared to most traditional C8 columns, due to unique polar end-capping combined with a densely bonded C8 ligands. Synergi ydro-rp vs. Traditional 5 µm C8 +3 Greater retention, polar selectivity! Increased hydrophobic retention! App ID App ID 5 Columns: Synergi µm ydro-rp 80Å Traditional 5 µm C8 00Å Part No.: 00F-375-E0 Mobile Phase: 0 mm Potassium phosphate, p 7.0 /Methanol (60:0) Flow Rate:.0 ml/min Temperature: C Detection: 0 nm Sample:. Phenylephrine. Phenylpropanolamine 3. Pseudoepherine. Methylparaben

5 Synergi FUSION-RP Balanced Non-polar Selectivity with Polar Retention For improved run times for extremely hydrophobic compounds and increased retention for polar compounds, Synergi Fusion-RP provides the right balance for mixtures of non-polar and polar analytes. Synergi µm Fusion-RP vs. Traditional 5 µm C8 Longer Polar Retention 3 Less ydrophobic Retention Balanced retention! min App ID 80 Columns: Synergi µm Fusion-RP Traditional 5 µm C8 Part No.: 00F--E0 Mobile Phase: 0 mm Potassium Phosphate, p.5 / Acetonitrile (75:5) Flow Rate:.0 ml/min Detection: 0 nm Sample:. Maleic acid. Chlorpheniramine 3. Triprolidine. Diphenhydramine min 5

6 Synergi POLAR-RP Strong-polar Retention with Aromatic Selectivity ighly polar compounds are difficult to retain on traditional C8 columns. A specialized ether-linked phenyl group gives better selectivity for aromatic compounds while providing maximum polar retention. Synergi µm Polar-RP vs. Waters SymmetryShield C8 Improved selectivity! App ID 55 App ID 85 Columns: Synergi µm Polar-PR Waters 5 µm SymmetryShield C8 Part No.: 00F-336-E0 Mobile 0 mm Potassium phosphate Phase: p 3 / Methanol (50:50) Flow Rate:.0 ml/min Detection: 30 nm Injection: µl Temperature: C Sample:. Metaproterenol (0. µg). Pindolol (0.6 µg) 3. Metoprolol (0.5 µg). Alprenolol (0.3 µg) 5. Propranolol (0.0 µg) 6. Ethylparaben (0. µg) 6

7 PFP() Luna PFP() Separate Compounds that are Isomeric, Aromatic, & alogenated When a truly different type of reversed phase selectivity is needed, Luna PFP() is an excellent choice, even under typical C8 mobile phase conditions. A pentafluorophenyl-propyl ligand uses multiple mechanisms of interaction for improved separation of isomers, aromatics, and halogenated compounds. Positional Isomers of alogenated Phenols alogens can radically increase the polarity of a compound, thus decreasing typical retention characteristics. Luna PFP() columns retain, discriminate, and separate halogens easily. Luna 3 µm PFP() Baseline separation min 9 0 App ID 696 Luna 3 µm C8() min & 0 Coelution! App ID 697 Columns: Luna 3 µm PFP() Luna 3 µm C8() Part Nos.: 00F-7-E0 00F-5-E0 Mobile Phase: A: 0. % Formic acid in Water B: 0. % Formic acid in Acetonitrile Gradient: A/B (60:0) to (50:50) in 0 min Flow Rate:.0 ml/min Temperature: C Detection: 5 nm Sample:.,3-Dimethylphenol.,5-Dimethylphenol 3.,6-Dimethylphenol. 3,-Dimethylphenol 5. 3,5-Dimethylphenol 6.,5-Dichlorophenol 7.,6-Dichlorophenol 8. 3,-Dichlorophenol 9. 3,5-Dichlorophenol 0.,-Dibromophenol Positional Isomers of Methylacetophenone Positional changes on an analyte of interest may effect the compound s dipole moment. This change can be readily noticed by the highly electronegative fluorine (F) atom and other retention mechanisms. Luna 3 µm PFP() mechanisms of interaction provide good resolution of isomers! 3 App ID 698 Luna 3 µm C8() mechanism of interaction unable to resolve isomers! 3 App ID 699 Columns: Luna 3 µm PFP() Luna 3 µm C8() Part Nos.: 00F-7-E0 00F-5-E0 Mobile Phase: Water/ Methanol (50:50) Flow Rate:.0 ml/min Temperature: C Detection: 5 nm Sample:. o-methylacetophenone. m-methylacetophenone 3. p-methylacetophenone min min 7

8 Ordering Information Gemini -NX 3 μm Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 50 x.0 x.0* C8 00B-53-B0 00F-53-B0 AJ μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.6 50 x.6 x 3.0* C8 00B-53-E0 00F-53-E0 AJ for ID: mm 5 μm Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 x.0* C8 00B-5-B0 AJ μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.6 50 x.6 50 x.6 x 3.0* C8 00B-5-E0 00F-5-E0 00G-5-E0 AJ for ID mm Luna PFP() 3 μm Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 00 x.0 50 x.0 x.0 * PFP() 00B-7-B0 00D-7-B0 00F-7-B0 AJ μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x x.6 x.0* x 3.0 * PFP() 00F-7-Y0 00F-7-E0 AJ0-836 AJ mm 5 μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x 3.0 x.0* PFP() 00F-8-Y0 AJ μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.6 50 x.6 x 3.0* PFP() 00F-8-E0 00G-8-E0 AJ0-837 for ID: mm Synergi.5 µm igh Speed Technology (ST) Columns (mm) Phases 50 x.0 00 x.0 ydro-rp 00B-387-B0 00D-387-B0 Polar-RP 00B-37-B0 00D-37-B0 Fusion-RP 00B-3-B0 00D-3-B0 µm Narrow Bore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x x 3.0 x.0* ydro-rp 00F-375-Y0 00G-375-Y0 AJ0-750 Polar-RP 00F-336-Y0 00G-336-Y0 AJ Fusion-RP 00F--Y0 00G--Y0 AJ µm Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 30 x.0 50 x.0 75 x.0 50 x.0 x.0* ydro-rp 00A-375-B0 00B-375-B0 00C-375-B0 00F-375-B0 AJ0-750 Polar-RP 00A-336-B0 00B-336-B0 00C-336-B0 00F-336-B0 AJ Fusion-RP 00A--B0 00B--B0 00C--B0 00F--B0 AJ If Gemini, Luna, and Synergi analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase, and dimensions, send in your comparative data within 5 days and keep the Luna column for FREE. µm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.6 75 x.6 50 x.6 50 x.6 x 3.0* ydro-rp 00B-375-E0 00C-375-E0 00F-375-E0 00G-375-E0 AJ0-75 Polar-RP 00B-336-E0 00C-336-E0 00F-336-E0 00G-336-E0 AJ Fusion-RP 00B--E0 00C--E0 00F--E0 00G--E0 AJ for ID: mm *SecurityGuard Analytical cartridges require holder, Part No.: KJ0-8 Additional analytical and preparative dimensions available. Contact us or visit Protect Your PLC Column SecurityGuard Cartridge System Extends your PLC column lifetime Won t alter your chromatography Compatible with virtually all PLC columns To learn more about protecting your PLC column investment visit Australia t: f: auinfo@phenomenex.com Canada (800) (30) info@phenomenex.com Germany anfrage@phenomenex.com Luxembourg +3 (0) (0) nlinfo@phenomenex.com Puerto Rico (800) 5-PLC (30) info@phenomenex.com Austria t: f: anfrage@phenomenex.com Denmark dkinfo@phenomenex.com Ireland eireinfo@phenomenex.com Netherlands nlinfo@phenomenex.com United Kingdom ukinfo@phenomenex.com Belgium t: +3 (0) f: +3 (0) beinfo@phenomenex.com France franceinfo@phenomenex.com Italy italiainfo@phenomenex.com New Zealand nzinfo@phenomenex.com All other countries: Corporate Office USA (30) (30) info@phenomenex.com Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com BR _I 8