Inertsil ODS-EP Technical Information

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1 Technical Information

2 Advantages of The selectivity is completely different from those of conventional columns such as ODS column due to its specific polar group in the stationary phase. Durability can be obtained by the high density bonding. Superior peak shapes of both acids and bases can be obtained because of neutral bonded phase and low residual silanol groups. Silica gel of high-purity is used as the support material, which minimizes effects of any remaining metal ions. Specifications of Partical diameter Shape Support Silica Gel Specific surface area Pore diameter Purity 5μm Spherical 450m /g 00Å Chemical Modification Bonded group Embededpolar Octadecyl group ODS Carbon content 9 %

3 () Naphthalene sample ~ for measurement retention and peak shape ~ 4 Conditions Column dimensions: 4.6 mm I.D x 50 mm length Mobile phase: Acetonitrile : Water =65 : 5 Flow rate: ml / min Column temperature: 40 Detection: UV 54 nm Peak identification. Acetophenone. Benzene. Toluene 4. Naphthalene

4 Comparison of retention between and commercial embedded columns Discovery RP Amide C Symmetry Shield RP Zorbax Bonus-RP Xterra RP Hypersil HyPURITY Advance

5 Comparison of retention between and other type commercial columns Inertsil ODS Inertsil C Aqua C8(polar endcapping) Inertsil Ph-

6 () Selectivity test sample ~ for measurement selectivity ~ 4 Conditions Column dimensions: 4.6 mm I.D x 50 mm length Mobile phase: Acetonitrile : Water = 70 : 0 Flow rate: ml / min Column temperature: 40 Detection: UV 65 nm Peak identification. Ethylbenzene C H 5 5. Naphthalene n-propylbenzene (CH ) CH 4. (CH ) CH 5. Anthracene

7 Comparison of selectivity between and commercial embedded columns Anthracene + Anthracene Discovery RP Amide C Anthracene Symmetry Shield RP8 + Anthracene Zorbax Bonus-RP Xterra RP8 Anthracene Anthracene Hypersil HyPURITY Advance

8 Comparison of selectivity between and other type commercial columns Anthracene Anthracene Anthracene Inertsil ODS Inertsil C8- Anthracene Anthracene Aqua C8(polar endcapping) Inertsil Ph-

9 Comparison of retention and selectivity between and commercial embedded columns k' () α(anthracene/butylbenzene) Symmetry Shield RP8 Xterra RP8 Discovery RP Amide C6 Zorbax Bonus-RP Hypersil HyPURITY Advance Inertsil ODS- Inertsil C8- Inertsil Ph- Aqua C8 The selectivity of is completely different from those of conventional columns such as ODS column due to its specific polar group in the bonded phase.

10 () Tanaka-test sample ~ for measurement selectivity, hydrogen bonding capacity and hydrophobicity ~ Conditions Column dimensions: 4.6 mm I.D x 50 mm length Mobile phase: Methanol : Water = 80 : 0 Flow rate: ml / min Column temperature: 40 Detection: UV 54 nm Peak identification. Uracil. Caffeine. Phenol n-amylbenzene 6. o-terphenyl 7. Triphenylene

11 Comparison of selectivity between and commercial embedded columns Discovery RP Amide C Symmetry Shield RP Xterra RP Zorbax Bonus-RP Hypersil HyPURITY Advance

12 Comparison of selectivity between and other type commercial columns Inertsil ODS Aqua C8(polar endcapping) Inertsil C8- Inertsil Ph-

13 α(triphenylene/o-terphenyl) α(amylbenzene/butylbenzene) α(caffeine/phenol) Characterization of and commercial embedded columns by Tanaka test Hydrogen bonding capacity Symmetry Shield RP8 Xterra RP8 Discovery RP Amide C6 Hydrophobicity () Zorbax Bonus-RP Hypersil HyPURITY Advance () Caffeine shows no retention Conditions Column dimensions: 4.6 mm I.D x 50 mm length Mobile phase: Methanol : Water = 80 : 0 Flow rate: ml / min Column temperature: 40 Detection: UV 54 nm CH O N O CH N CH N Caffeine N O H Phenol 0.50 ( C H ) CH ( C H ) 4 CH 0.00 Symmetry Shield RP8 Xterra RP8 Discovery RP Amide C6 Zorbax Bonus-RP Hypersil HyPURITY Advance n-amylbenzene Steric selectivity o-terphenyl Triphenylene 0.00 Symmetry Shield RP8 Xterra RP8 Discovery RP Amide C6 Zorbax Bonus-RP Hypersil HyPURITY Advance

14 (4) Pyridine-Phenol sample ~ for measurement of inertness to basic compounds ~ Conditions Column dimensions: 4.6 mm I.D x 50 mm length Mobile phase: Methanol : 0mM Phosphate buffer(ph6.8) =0 : 70 Flow rate: ml / min Column temperature: 40 Detection: UV 54 nm Peak identification. Uracil. Pyridine. Phenol

15 Comparison of inertness to pyridine between and commercial embedded columns Pyridine Phenol Pyridine Phenol Discovery RP Amide C6 Pyridine Phenol Pyridine Phenol Symmetry Shield RP Zorbax Bonus-RP Pyridine Phenol Pyridine Phenol Xterra RP Hypersil HyPURITY Advance

16 (5) Acid test sample ~ for measurement of inertness to acidic compounds ~ Conditions Column dimensions: 4.6 mm I.D x 50 mm length Mobile phase: 0.% Phosphoric acid(ph.) Flow rate: ml / min Column temperature: 40 Detection: UV 0 nm Peak identification. Formic acid. Acetic acid

17 Comparison of inertness to acids between and commercial embedded columns Formic acid Formic acid Acetic acid Acetic acid Discovery RP Amide C6 Acetic acid Acetic acid Formic acid Symmetry Shield RP Zorbax Bonus-RP Formic acid Acetic acid Formic acid +Acetic acid Xterra RP Hypersil HyPURITY Advance

18 (6) Oxine-copper sample ~ for measurement of inertness to chelating compounds ~ Conditions Column dimensions: 4.6 mm I.D x 50 mm length Mobile phase: Acetonitrile : 0.% Phosphoric acid(ph.) =0 : 90 Flow rate: ml / min Column temperature: 40 Detection: UV 54 nm Peak identification. Oxine-copper

19 Comparison of inertness to oxine-copper between and commercial embedded columns Discovery RP Amide C Symmetry Shield RP Zorbax Bonus-RP Xterra RP8.0.0 Hypersil HyPURITY Advance

20 Relative retention time of naphthalene(%) Comparison of durability among, ODS column and Amide column ODS test column Amide test column Flow time of 0.% Phosphoric acid (ph) ( hours ) Test condition Column: 4.6 mm I.D. x 50 mm Flow rate: ml / min Column temperature: 0

21 The selectivity is completely different from those of conventional columns such as ODS column, but the retention is close to conventional columns. is useful for the samples which are not separated by conventional columns. High durability can be obtained even if strong acid was used as mobile phase. is useful for the LC/MS analysis. Superior peak shapes of both acids and bases can be obtained because of neutral bonded phase and low residual silanol groups. Silica gel of high-purity is used as the support material, which minimizes effects of any remaining metal ions.

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