Hamilton. Reversed-Phase Anion Exchange Cation Exchange Ion Exclusion

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1 HPLC Columns Agilent for Small Molecule InfinityLab Poroshell 0 ZORBAX Eclipse Plus ZORBAX Eclipse XDB ZORBAX 80Å StableBond ZORBAX 80Å Extend-C8 ZORBAX Bonus-RP ZORBAX Eclipse PAH ZORBAX HILIC Plus ZORBAX NH ZORBAX Rx Pursuit XRs Polaris Agilent BioHPLC Poroshell 00 AdvanceBio RP-mAb AdvanceBio Glycan Mapping InfinityLab Poroshell 0 AdvanceBio Peptide Mapping PLRP-S Zorbax 00Å StableBond AdvanceBio SEC ProSEC 00S Bio SEC- Bio SEC-5 ProSEC 00S Agilent GPC/SEC Aqueous GPC/SEC Columns Aqueous Polymer Standards Organic GPC/SEC Columns Organic Polymer Standards Concise Separations Carbohydrate Analysis Organic Acid Analysis Protein Analysis Amino Acid Analysis Ion Analysis Hamilton Reversed-Phase Anion Exchange Cation Exchange Ion Exclusion Imtakt Presto FF-C8 Cadenza CD-C8 Intrada Amino Acids Separation Scherzo C8 Mutli-Mode Jordi Jordi Resolve Organic GPC Jordi Resolve Aqueous GPC Perkin Elmer Brownlee Regis Regis Pirkle Chiral Columns Immobilized Artificial Chromatography Restek Raptor Ultra HPLC Force Roc Millipore Sigma Ascentis Express Chromalith RP-8 endcapped Thermo Scientific Accucore Hypersil Gold Vydac HPLC/MS Columns 0TP and 0TP Columns for Peptides & Polypeptides Zirchrom Columns ZIrconia Based HPLC Columns ProTain In-Line Protein Removal System For Peak Performance 58

2 Featured Products 60 Agilent InfinityLab Poroshell 0 9 Concise Separations Columns Imtakt Scherzo C8 Family ODS + Anion Ligand superficially porous particle for increased efficiency and decreased back pressure specializing in Organic Acid Analysis Carbohydrate Analysis Fermentation Monitoring Anion Analysis ODS + Cation Ligand The Scherzo C8 Family consists of not only ODS ligands, but also anion ligands and cation ligands. It also provides reversed-phase modes, both ion exchange modes, and normal phase mode. 0 Restek Raptor Biphenyl 9 Millipore Sigma Chromolith RP-8 endcapped CHROM TECH RELATED PRODUCTS Ideal for bioanalytical applications like drug and metabolite analyses Chromolith column consists of a single rod of high-purity polymeric silica gel with a bimodal pore structure of macro and mesopores Precolumn Filters, page 89 Economical protection for analytical columns in HPLC and UHPLC Notes from the Support Desk How can we help you? Q Looking for a U.S. Pharmacopeia column phase? ie: L, L, L Looking for a column recommendation based on your specific application? Looking for a column alternative? Can t find the specific dimensions listed for your column? Can t find an Alltech or Vydac column? Agilent A-Line Quick Connect Fitting, page The proprietary Agilent design features a spring-loaded mechanism to ensure proper ferrule placement in the column port. A zero dead volume fingertight connection is achieved every time. Contact Chrom Tech Support Desk for a quote and technical recommendation support@chromtech.com A HPLC Columns

3 HPLC COLUMNSAgilent Small Molecule Columns Agilent InfinityLab Poroshell 0 HPLC Columns µm frit, for rugged performance with dirty samples High efficiency and high resolution Up to 50% less back pressure than sub µm particle Compatible with 00/600 bar LC s, as well as UHPLCs These high efficiency, high resolution columns can be used on any type of LC. The porous outer layer and solid core limit diffusion distance and improve separation speed while the narrow particle size distribution improves efficiency and resolution. The Poroshell 0 family is expanding. Now, there are Three particle sizes (.9,.7, and µm) so you can choose the option to best fit your needs. We recognize that you have your own quality control demands and that you test your method with multiple batches of material to ensure it s robust and reproducible. To support your requirements, we offer all-in-one kits that put method validation columns from three separate lots right at your fingertips. Simply add the letter "K" to the end of the part number. 0.5 µm Poroshell 0 Particle (shown with column ID tag) Expanded Offering! Now available in three particle sizes:.9 µm (NEW!).7 µm µm AGILENT INFINITY LAB POROSHELL 0 BONDED PHASE SPECIFICATIONS BONDED PHASE PORE SIZE TEMP LIMIT ph RANGE ENDCAPPED EC-C8, EC-C8, Phenyl-Hexyl 0Å 60 C Yes SB-C8 0Å 90 C No SB-C8, SB-Aq 0Å 80 C No Bonus RP 0Å 60 C Triple HILIC 0Å 60 C No EC-CN 0Å 60 C Double PFP 0Å 60 C Yes HPH-C8, HPH-C8* 0Å 60 C.0.0 Yes Specifications represent typical values only *New Poroshell HPH-C8 and HPH-C8 technologies are made by chemically modifying Poroshell particles using proprietary technology designed to give high ph stability. Therefore, the pore sizes differ from the other phases in the Poroshell family. TECH TIP Optional Column ID Tag* Column ID tags give you at a glance details about the last injection date, number of injections, and the maximum temperature used. To order, simply add a "T" to the end of any Agilent InfinityLab Poroshell 0 HPLC Column. * Column ID Tags are only compatible with Agilent Infinity II thermostatted column compartment with column identification option. AGILENT INFINITY LAB POROSHELL 0.7 µm SIZE (mm) EC-C8 EC-C8 SB-C8 SB-C8 HPH-C8 HPH-C8 SB-Aq BONUS RP EC-CN.6 x x x x x x 5 Guard, /pk x x x x x x 5 Guard, /pk x x x x x x 5 Guard /pk Chrom Tech your chromatography specialists

4 Agilent Small Molecule Columns HPLC COLUMNS AGILENT INFINITY LAB POROSHELL 0.7 µm SIZE (mm) PFP PHE-HEX HILIC.6 x x x x 5 Guard, /pk x x x x 5 Guard, /pk x x x x 5 Guard, /pk UHPLC Guard Column /pk Complete guard assembly No holder required AGILENT INFINITY LAB POROSHELL 0 µm SIZE (mm) EC-C8 EC-C8 PFP PHE-HEX HILIC.6 x x x x x 5 Guard, /pk x x x x x 5 Guard, /pk x x x x x 5 Guard, /pk AGILENT INFINITY LAB POROSHELL 0.9 µm SIZE (mm) EC-C8 EC-C8 HILIC PFP PHE-HEX HPH-C8.0 x x x Guard, /pk x x x x Guard, /pk TECH TIP Column ID Tag* Standard on Poroshell 0.9 µm Column ID tags give you at a glance details about the last injection date, number of injections, and the maximum temperature used. * Column ID Tags are only compatible with Agilent Infinity II thermostatted column compartment with column identification option. NEW! AGILENT INFINITY LAB POROSHELL 0.7 µm SIZE (mm) HILIC-Z HILIC-OH5.6 x x x x 5 Guard, /pk x x x x 5 Guard, /pk x x 50 PEEK lined x x 00 PEEK lined x x 50 PEEK lined x 5 Guard, /pk NEW Agilent Infinity Lab Poroshell 0 HILIC-Z Columns Novel zwitterionic stationary phase bonded to.7 µm Poroshell 0 particles PEEK-lined column option for excellent peak shape and recovery of particularly challenging charged compounds High ph and temperature stability: Up to ph and 80 C NEW Agilent Infinity Lab Poroshell 0 HILIC-OH5 Columns Novel poly-hydroxy fructan phase bonded to.7 µm Poroshell 0 particles Offer alternative selectivity to HILIC and HILIC-Z phases TECH TIP Optional Column ID Tag* Column ID tags give you at a glance details about the last injection date, number of injections, and the maximum temperature used. To order, simply add a "T" to the end of any Agilent InfinityLab Poroshell 0 HILIC-Z or HILIC-OH5 Column. * Column ID Tags are only compatible with Agilent Infinity II thermostatted column compartment with column identification option. Authorized Distributor

5 HPLC COLUMNSAgilent ZORBAX Eclipse Plus Agilent ZORBAX Eclipse Plus The ideal column for method development excellent for a wide range of compounds High level of performance peak shape, efficiency, resolution, and lifetime with all sample types: acids, bases and neutrals Agilent ZORBAX Eclipse Plus columns provide the ultimate in performance for silicabased columns. Peak shape is excellent for the most challenging basic compounds, improving efficiency and resolution with these sample types. These results are achieved by improvements in the silica manufacturing and bonding technology, which is completely controlled by Agilent. Because of their high level of performance, Eclipse Plus columns are the ideal first choice for method development of all samples. If you need to achieve fast method development and superior productivity, then choose a column with high-resolution.8 µm particles. For standard methods, conventional 5 µm and Rapid Resolution.5 µm columns are your best choice. With all particle sizes, easy method transfer is possible. O O O O O CH Si CH CH Si CH CH CH Si CH CH Si CH CH CH Si CH AGILENT ZORBAX ECLIPSE PLUS COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE SURFACE AREA ph RANGE* ENDCAPPED TEMP LIMIT CARBON LOAD ZORBAX Eclipse Plus C8 95Å 60 m /g Double 60 ºC 9% ZORBAX Eclipse Plus C8 95Å 60 m /g Double 60 ºC 7% ZORBAX Eclipse PAH 95Å 60 m /g No 60 ºC % ZORBAX Eclipse Plus Ph-Hex 95Å 60 m /g Double 60 ºC 9% Specifications represent typical values only. *Column lifetime will be reduced significantly at ph > 7 and temperatures > 0 C. At ph 6-9, highest column stability for all silica based columns is obtained by operating at temperatures < 0 C and using lower buffer concentrations in the range of M, especially with phosphate and carbonate buffers. ZORBAX Eclipse Plus Columns Rapid analysis of an analgesic tablet, selectivity differences at ph and ph 7 Column A: Gradient: Sample: Eclipse Plus C x 50 mm, 5 µm 0 60% B/ min ph.7:a: 0.% Formic acid B: 0.% fa in ACN ph 7.0: A: 0 mm Na phosphate B: ACN generic Excedrin tablet Both Eclipse Plus C8 and C8 can be used over a wide ph range to optimize selectivity or analysis time. Acetaminophen. Caffeine. Acetylsalicylic acid. Unknown min min. ph.7 ph min 6 Chrom Tech your chromatography specialists

6 Agilent ZORBAX Eclipse Plus HPLC COLUMNS DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) Eclipse Plus C8 (USP L) Eclipse Plus C8 (USP L7) Eclipse Plus Phenyl-Hexyl (USP L) Eclipse PAH (USP L) Analytical.6 x Analytical.6 x Analytical.6 x Analytical.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Guard Column Cartridges, /pk.6 x High Performance ZORBAX Guard Fittings Kit Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x UHPLC Guard, 600 bar, /pk.6 x Solvent Saver.0 x Solvent Saver.0 x Solvent Saver Plus.0 x Solvent Saver Plus.0 x Guard Column Cartridges, /pk.6 x High Performance ZORBAX Guard Fittings Kit Solvent Saver RRHD, 00 bar.0 x Solvent Saver RRHD, 00 bar.0 x Solvent Saver RRHD, 00 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x UHPLC Guard Column, 00 bar, /pk.0 x Narrow Bore. x Narrow Bore. x Narrow Bore. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Guard Cartridges, /pk. x High Performance ZORBAX Guard Fittings Kit Narrow Bore RRHD, 00 bar. x Narrow Bore RRHD, 00 bar. x Narrow Bore RRHD, 00 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x UHPLC Guard Column, 00 bar, /pk. x UHPLC Guard Column Complete guard assembly No holder required High Performance ZORBAX Guard Fittings Kit Authorized Distributor Cartridges sold separately

7 HPLC COLUMNSAgilent ZORBAX Eclipse XDB Agilent ZORBAX Eclipse XDB Four selectivity choices for method development optimization Good peak shape for basic, acidic and neutral compounds High performance over a wide ph range ph 9 Particle sizes from.8 to 7 µm Long lifetime with extra Dense Bonding (XDB) and double endcapping O O CH Si CH CH Si CH CH AGILENT ZORBAX ECLIPSE XDB COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE Specifications represent typical values only SURFACE AREA ph RANGE* ENDCAPPED TEMP LIMIT CARBON LOAD ZORBAX Eclipse XDB C8 80Å 80 m /g Double 60 ºC 0% ZORBAX Eclipse XDB C8 80Å 80 m /g Double 60 ºC 7.6% ZORBAX Eclipse XDB-Phenyl 80Å 80 m /g Double 60 ºC 7.% ZORBAX Eclipse XDB-CN 80Å 80 m /g Double 60 ºC.% *Eclipse XDB columns are designed for operation over a wide ph range. At ph 6 9, highest columns stability for all silica based columns is achieved by operating at temperatures < 0 C and using low buffer concentrations in the range of M. O O O CH Si CH CH Si CH CH CH Si CH extra Densely Bonded and Double Endcapped Eclipse XDB Bonded Phase DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) STANDARD COLUMNS (NO SPECIAL HARDWARE REQUIRED) Eclipse XDB- C8 (USP L) Eclipse XDB- C8 (USP L7) Eclipse XDB- Phenyl (USP L) Eclipse XDB- CN (USP L0) SemiPrep 9. x SemiPrep Guard Cartridge 9. x * * * * SemiPrep Guard Hardware Kit Analytical.6 x Analytical.6 x Analytical.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Guard Cartridges, /pk.6 x High Performance ZORBAX Guard Fittings Kit Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x UHPLC Guard Column, 00 bar/pk.6 x Solvent Saver.0 x Solvent Saver.0 x Solvent Saver Plus.0 x Solvent Saver Plus.0 x Solvent Saver Plus.0 x Guard Cartridges, /pk.6 x High Performance ZORBAX Guard Fittings Kit *These columns are packed with Eclipse XDB-C8, 5 µm 6 Chrom Tech your chromatography specialists

8 Agilent ZORBAX Eclipse XDB HPLC COLUMNS DESCRIPTION STANDARD COLUMNS (NO SPECIAL HARDWARE REQUIRED) SIZE (mm) PARTICLE SIZE (µm) Eclipse XDB- C8 (USP L) Eclipse XDB- C8 (USP L7) Eclipse XDB- Phenyl (USP L) Eclipse XDB- CN (USP L0) Solvent Saver RRHD, 00 bar.0 x Solvent Saver RRHD, 00 bar.0 x Solvent Saver RRHD, 00 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x UHPLC Guard Column, 00 bar, /pk.0 x Narrow Bore. x Narrow Bore. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Guard Cartridges, /pk. x High Performance ZORBAX Guard Fittings Kit Narrow Bore RRHD, 00 bar. x Narrow Bore RRHD, 00 bar. x Narrow Bore RRHD, 00 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x UHPLC Guard Column, 00 bar, /pk. x MicroBore RR.0 x MicroBore RR.0 x MicroBore RR.0 x MicroBore Guard Column, /pk.0 x CAPILLARY GLASS-LINED COLUMNS Capillary 0.5 x Capillary 0.5 x Capillary RR 0.5 x Capillary RR 0.5 x Capillary 0. x Capillary 0. x Capillary RR 0. x Capillary 0.5 x Capillary 0. x UHPLC Guard Column Complete guard assembly No holder required High Performance ZORBAX Guard Fittings Kit Cartridges sold separately Authorized Distributor

9 HPLC COLUMNSAgilent ZORBAX 80Å StableBond Agilent ZORBAX 80Å StableBond Longest column lifetime and best reproducibility for low ph separations down to ph R Patented stable column chemistry allows use at high temperature and low ph without degradation O Si R Six different bonded phases provide broad selectivity SB-C8, SB-C8, SB- CN, SB-Phenyl, SB-C and SB-Aq High purity (Type B) silica for good peak shape OH R Agilent ZORBAX StableBond columns use patented, unique, nonfunctional silanes with bulky disobutyl (SB-C8) or disopropyl (SB-C8, SB-C, SB-Phenyl, SB-CN, and SB-Aq) side chain groups that sterically protect the key siloxane bond to the silica surface from hydrolytic attack at low ph. StableBond packing materials are not endcapped in order to provide exceptional stability and to maximize lifetime and reproducibility under acidic mobile phase conditions. O OH R Si R R AGILENT ZORBAX 80Å STABLEBOND COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE Specifications represent typical values only SURFACE AREA ph RANGE* ENDCAPPED TEMP LIMITS* CARBON LOAD ZORBAX SB-C8 80Å 80 m /g No 90 C 0% ZORBAX SB-C8 80Å 80 m /g No 80 C 5.5% ZORBAX SB-C 80Å 80 m /g No 80 C % ZORBAX SB-Phenyl 80Å 80 m /g No 80 C 5.5% ZORBAX SB-CN 80Å 80 m /g No 80 C % ZORBAX SB-Aq 80Å 80 m /g No 80 C proprietary *StableBond columns are designed for optimal use at low ph. At ph 6 8, highest column stability for all silicabased columns is obtained by operating at temperatures < 0 C and using lower buffer concentrations in the range of M. At mid-range ph, Eclipse Plus, Eclipse XDB and Bonus-RP are recommended. O Sterically Protected StableBond Bonded Phase R Si R R StableBond SB-C8 shows excellent stability at low ph and high temperature (ph 0.8, 90 C) StableBond SB-C8 (Diisobutyl-C8) Column: Column: ZORBAX SB-C x 50 mm, 5 µm ZORBAX Rx-C x 50 mm, 5 µm ZORBAX Rx-C8 (Dimethyl-C8) Competitor A-C8 Mobile Phase: Temperature: 90 C 50% Methanol/50% Water with.0% TFA Test Solute: Toluene As an indicator of column breakdown, retention time of toluene was measured after purging the column with mobile phase. Only the StableBond SB-C8 is unchanged after three working months of use under these very low ph (0.8) and high temperature (90 C) conditions. ZORBAX Rx-C8 also provides a stable matrix, and can be used as an alternative selectivity to StableBond SB-C8 Relative Retention ,000 0,000 5,000 0,000 5,000 0,000 Column Volumes of Mobile Phase Purge LCSB00 Competitor B-C8 Competitor C-C8 Competitor D-C8 Competitor E-C8 66 Chrom Tech your chromatography specialists

10 Agilent ZORBAX 80Å StableBond HPLC COLUMNS DESCRIPTION SIZE (mm) STANDARD COLUMNS (NO SPECIAL HARDWARE REQUIRED) PARTICLE SIZE (µm) SB-C8 (USP L) SB-C8 (USP L7) SB-CN (USP L0) SB-C (USP L56) SB-Phenyl (USP L) SemiPrep 9. x SemiPrep 9. x SemiPrep 9. x SemiPrep 9. x SemiPrep Guard Cartridges, /pk 9. x SemiPrep Guard Hardware Kit 9. x Analytical.6 x Analytical.6 x Analytical.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Guard Cartridges, /pk.6 x High performance ZORBAX guard fittings kit Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x UHPLC Guard Columns, 600 bar, /pk.6 x Solvent Saver.0 x Solvent Saver.0 x Solvent Saver Plus.0 x Solvent Saver Plus.0 x Solvent Saver Plus.0 x Guard Cartridges, /pk.6 x High performance ZORBAX guard fittings kit Solvent Saver RRHD, 00 bar.0 x Solvent Saver RRHD, 00 bar.0 x Solvent Saver RRHD, 00 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x UHPLC Guard Columns, 00 bar, /pk.0 x Unless indicated, column pressure limit is 00 bar SB-Aq (Continued on next page) UHPLC Guard Column Complete guard assembly No holder required High Performance ZORBAX Guard Fittings Kit Cartridges sold separately Authorized Distributor

11 HPLC COLUMNSAgilent ZORBAX 80Å StableBond DESCRIPTION SIZE (mm) STANDARD COLUMNS (NO SPECIAL HARDWARE REQUIRED) PARTICLE SIZE (µm) SB-C8 (USP L) SB-C8 (USP L7) SB-CN (USP L0) SB-C (USP L56) SB-Phenyl (USP L) Narrow Bore. x Narrow Bore. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Guard Cartridge, /pk. x High performance ZORBAX guard fittings kit Narrow Bore RRHD, 00 bar. x Narrow Bore RRHD, 00 bar. x Narrow Bore RRHD, 00 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x UHPLC Guard Column, 00 bar, /pk. x MicroBore RR.0 x MicroBore RR.0 x MicroBore RR.0 x MicroBore Guard Column, /pk.0 x PREPHT CARTRIDGE COLUMNS (REQUIRE ENDFITTINGS KIT ) PrepHT Cartridge. x PrepHT Cartridge. x PrepHT Cartridge. x PrepHT Cartridge. x PrepHT Cartridge. x PrepHT Cartridge, /pk 7.0 x Guard Cartridge Hardware PrepHT Endfittings, /pk AGILENT CARTRIDGE COLUMNS (REQUIRE HARDWARE KIT 50-85) Analytical.6 x Analytical.6 x Rapid Resolution.6 x Guard Cartridges, 0/pk.0 x Cartridge Holder Unless indicated, column pressure limit is 00 bar SB-Aq TECH TIP ZORBAX StableBond SB-C8, SB-C8 and SB-Aq phases are also available in Poroshell 0 (see pg 60) 68 Chrom Tech your chromatography specialists

12 Agilent ZORBAX 80Å StableBond HPLC COLUMNS DESCRIPTION SIZE (mm) STANDARD COLUMNS (NO SPECIAL HARDWARE REQUIRED) PARTICLE SIZE (µm) SB-C8 (USP L) SB-C8 (USP L7) Rapid Resolution HT.6 x Rapid Resolution HT, /pk.6 x Narrow Bore RRHT. x Narrow Bore RRHT, /pk. x CAPILLARY GLASS-LINED COLUMNS Capillary 0.5 x Capillary 0.5 x Capillary 0.5 x Capillary RR 0.5 x Capillary RR 0.5 x Capillary 0. x Capillary 0. x Capillary 0. x Capillary RR 0. x Agilent ZORBAX 80Å StableBond The high purity, low acidity silica provides excellent peak shape with acidic, basic and neutral compounds making StableBond columns an excellent choice for low ph method development. ZORBAX StableBond columns are compatible with all common mobile phases, including highly aqueous mobile phases. Five different bonded phases provide selectivity options A 5 Column A: Column B: Column C: ZORBAX SB-C x 50 mm, 5 µm ZORBAX SB-C x 50 mm, 5 µm ZORBAX SB-C x 50 mm, 5 µm B 5. Procaine. Lidocaine. d-cinchonine. Butacaine 5. Tetracaine Column D: ZORBAX SB-Phenyl x 50 mm, 5 µm Column E: Mobile Phase: ZORBAX SB-CN x 50 mm, 5 µm 0 00% B in 8.8 min A: 50 mm NaH PO, ph.5 in 95% H O/5% ACN B: 50 mm NaH PO, ph.5 in 7% H O/5% ACN C 5 Flow Rate:.0 ml/min Temperature: 6 C Detector: 5 nm D 5 SB-C is just one of the five different StableBond selectivity choices. In this example, optimum resolution is obtained with SB-C. All are based on the same high purity Rx-SIL. Selectivity changes are therefore dependent only on the bonded phases, making method development more reliable. E Time (min) LCSB00 Authorized Distributor

13 HPLC COLUMNS Agilent ZORBAX 80Å Extend C-8 Agilent ZORBAX 80Å Extend-C8 High efficiency and long life at ph up to.5 Bidentate bonding and double endcapping provides high ph stability More efficiency and better peak shape than polymer-based columns Improve retention, resolution and peak shape of basic compounds High sensitivity for LC/MS separations of peptides AGILENT ZORBAX 80Å EXTEND-C8 COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE Specifications represent typical values only *Temperature limits are 60 C up to ph 8, 0 C from ph 8.5 SURFACE AREA ph RANGE* ENDCAPPED TEMP LIMITS* CARBON LOAD ZORBAX Extend-C8 80Å 80 m /g.0.5 Double 60 C.5% **Above ph 6 highest column stability for all silica based columns is obtained by reducing the operating temperature to 0 C or below and using lower buffer concentrations ( M) or organic buffers. Long life at high ph with Extend-C8 Column: Mobile Phase: Flow Rate: Temperature: ZORBAX Extend-C x 50 mm, 5 µm 0% Methanol 80% 0. M Carbonate buffer, ph ml/min Ambient At high ph, columns will fail due to silica dissolution. The example here shows extended lifetime of ZORBAX Extend-C8 at high ph in comparison to competitor W. This was measured by the amount of dissolved silica. Amount of Dissolved Silica (mg) ZORBAX Extend-C8 Competitor W Volume Eluent (L) LC Chrom Tech your chromatography specialists

14 Agilent ZORBAX 80Å Extend C-8 HPLC COLUMNS Agilent ZORBAX 80Å Extend-C8 (cont.) DESCRIPTION SIZE (mm) STANDARD COLUMNS (NO SPECIAL HARDWARE REQUIRED) PARTICLE SIZE (µm) Extend-C8 (USP L) Analytical.6 x Analytical.6 x Analytical.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Guard Cartridge, /pk.6 x Guard Hardware Kit Solvent Saver.0 x Solvent Saver.0 x Solvent Saver Plus.0 x Solvent Saver Plus.0 x Solvent Saver Plus.0 x Solvent Saver RRHD, 00 bar.0 x Solvent Saver RRHD, 00 bar.0 x Solvent Saver RRHD, 00 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x Guard Cartridge, /pk.6 x Guard Hardware Kit Narrow Bore. x Narrow Bore. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RRHD, 00 bar. x Narrow Bore RRHD, 00 bar. x Narrow Bore RRHD, 00 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Guard Cartridge, /pk. x Guard Hardware Kit MicroBore RR.0 x MicroBore RR.0 x MicroBore RR.0 x MicroBore Guard, /pk.0 x Guard Hardware Kit Unless indicated, column pressure limit is 00 bar DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) PREPHT CARTRIDGE COLUMNS (REQUIRE ENDFITTINGS KIT ) Extend-C8 (USP L) PrepHT Cartridge. x PrepHT. x PrepHT. x PrepHT Endfittings, /pk PrepHT Guard Cartridge, /pk 7.0 x Guard Cartridge Hardware Authorized Distributor

15 HPLC COLUMNS Agilent ZORBAX Bonus-RP Agilent ZORBAX Bonus-RP Excellent peak shape for challenging basic compounds at low and mid ph Unique reversed-phase selectivity Novel bonding technology with embedded polar group and steric protection Usable in 00% aqueous mobile phases The Agilent ZORBAX Bonus-RP column has a polar amide group embedded in a long alkyl chain. This novel bonding reduces interactions between basic compounds and the silica support, improving peak shape for the most difficult basic compounds. Peak shape and column lifetime are further improved by triple endcapping. In addition, diisopropyl side groups provide steric protection against acid hydrolysis for good lifetime at low ph. The Bonus-RP column provides an alternate selectivity to C8 and C8 aklyl bonded phases. O CH Si CH O CH Si R Si R CH R Si R CH PG PG R R CH AGILENT ZORBAX BONUS-RP COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE Specifications represent typical values only *Temperature limits are 60 C up to ph 8, 0 C from ph 8 9. SURFACE AREA ph RANGE ENDCAPPED TEMP LIMITS* CARBON LOAD ZORBAX Bonus-RP 80Å 80 m /g Triple 60 C 9.5% O R Si R PG Unique, Polar Alkyl Bonus-RP Bonded Phase R ZORBAX Bonus-RP is stable at low and mid ph Column: Mobile Phase: ZORBAX Bonus-RP x 50 mm, 5 µm 60% 5 mm Phosphate Buffer, ph 7.0:0% ACN Toluene Plate Height, cm Competitive alkyl/amide Bonus-RP Flow Rate:.5 ml/min 0.00 Temperature: C Triple endcapping of Bonus-RP enhances stability at ph 7. Each 0,000 column volume is equivalent to approximately one working month ,000 0,000 5,000 0,000 5,000 0,000 5,000 Column Volume of Purge LCBR00 Dimethyl-C8/amide, Bonus-RP Column: Mobile Phase: Flow Rate: Temperature: ZORBAX Bonus-RP x 50 mm, 5 µm Aging: 50% MeOH 50% 0.% TFA Test: 80% MeOH 0% H O.0 ml/min Aging: 60 C Test: C Sterically protecting side groups provides low ph stability and longer column lifetime than similar polar alkyl bonded phases. Toluene Plate Height, cm Dimethyl-C8/amide Bonus-RP 0 0,000 0,000 0,000 0,000 Column Volume of Purge LCBR00 7 Chrom Tech your chromatography specialists

16 Agilent ZORBAX Bonus-RP HPLC COLUMNS Agilent ZORBAX Bonus-RP (cont.) DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) STANDARD COLUMNS (NO SPECIAL HARDWARE REQUIRED) Bonus-RP (USP L60) Analytical.6 x Analytical.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Guard Cartridge, /pk.6 x Guard Hardware Kit Solvent Saver.0 x Solvent Saver.0 x Solvent Saver Plus.0 x Solvent Saver Plus.0 x Solvent Saver HT, 600 bar.0 x Solvent Saver HT, 600 bar.0 x Guard Cartridge, /pk.6 x Guard Hardware Kit Narrow Bore. x Narrow Bore. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Rapid Resolution HD, 00 bar. x Rapid Resolution HD, 00 bar. x Rapid Resolution HD, 00 bar. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Guard Cartridge, /pk. x Guard Hardware Kit MicroBore RR.0 x MicroBore RR.0 x MicroBore RR.0 x MicroBore Guard, /pk.0 x Guard Hardware Kit PREPHT CARTRIDGE COLUMNS (REQUIRE ENDFITTINGS KIT ) PrepHT Cartridge. x PrepHT Cartridge. x PrepHT Cartridge. x PrepHT Cartridge. x PrepHT Cartridge. x PrepHT Endfittings, /pk PrepHT Guard Cartridge, /pk 7.0 x Guard Cartridge Hardware Unless indicated, column pressure limit is 00 bar Improved peak shape of basic compounds using Bonus-RP Column: TF Alkyl-C8.6 x 50 mm, 5 µm Mobile Phase: 75% 5 mm NH OAc, ph 5.5 Flow Rate: Temperature: 0 C Detector: TF ml/min 5 nm Time (min) Column: Time (min) ZORBAX Bonus-RP x 50 mm, 5 µm. Doxylamine. Chlorpheniramine. Tripolidine LCBR00 Mobile Phase: 80% 5 mm NH OAc, ph 5.5 0% ACN Flow Rate: Temperature: 0 C Detector:.5 ml/min 5 nm. Doxylamine. Chlorpheniramine. Tripolidine LCBR00 Bonus-RP eliminates peak tailing of these basic compounds in comparison to a typical alkyl C8 bonded phase. In the mid ph region, residual silanols can interact more strongly with basic compounds to cause peak tailing. The polar group in the Bonus-RP bonded phase eliminates peak tailing of these basic compounds by reducing interactions with residual silanols. 0 Authorized Distributor

17 HPLC COLUMNSAgilent Agilent ZORBAX Eclipse PAH DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) Eclipse PAH (USP L) Analytical.6 x Analytical.6 x Analytical.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Rapid Resolution HT, 600 bar.6 x Solvent Saver.0 x Guard Cartridges, /pk.6 x Guard Hardware Kit Narrow Bore. x Narrow Bore. x Narrow Bore RR. x Narrow Bore RRHT, 600 bar. x Narrow Bore RRHT, 600 bar. x Guard Cartridges, /pk. x Guard Hardware Kit ZORBAX Eclipse PAH Columns Extensive range of particle sizes (.8,.5 and 5 µm) and sizes for fast and high resolution separations Each batch of material is specifically tested with PAHs for maximum reproducibility under expected operating conditions Excellent performance using the high quality, improved silica of Eclipse Plus columns Great for applications requiring "shape selectivity" or the separation of geometric isomers ZORBAX ECLIPSE PAH COLUMN SPECIFICATIONS PORE SIZE SURFACE AREA p H RANGE END- CAPPED TEMP LIMIT 95Å 60 m /g No 60 ºC Specifications represent typical values only. Agilent ZORBAX HILIC Plus DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) ZORBAX HILIC Plus Analytical.6 x Analytical.6 x Narrow Bore. x Narrow Bore. x Rapid Resolution HD, 00 bar.0 x Rapid Resolution HD, 00 bar.0 x Rapid Resolution HD, 00 bar. x Rapid Resolution HD, 00 bar. x Rapid Resolution HD, 00 bar. x ZORBAX HILIC PLUS COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE SURFACE AREA p H RANGE ZORBAX HILIC Plus 95Å 60 m /g Specifications represent typical values only. Agilent ZORBAX NH DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) ZORBAX NH Analytical.6 x Analytical.6 x Analytical. x Guard Cartridges, /pk.6 x Guard Hardware Kit ZORBAX NH COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE SURFACE AREA p H RANGE END CAPPED ZORBAX NH 70Å 00 m /g Yes Specifications represent typical values only. 7 Chrom Tech your chromatography specialists

18 Agilent HPLC COLUMNS Agilent ZORBAX Rx AGILENT ZORBAX RX COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE SURFACE AREA ph RANGE* ENDCAPPED TEMP LIMIT CARBON LOAD ZORBAX Rx-C8 80Å 60 m /g No 60 ºC % ZORBAX Rx-C8 80Å 60 m /g No 80 ºC 5.5% Specifications represent typical values only. *At ph 6 9 highest column stability for all silica based columns is obtained by operating at temperatures < 0 C and using lower buffer concentrations in the range of M. DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) Rx-C8 (USP L) Rx-C8* (USP L7) Analytical.6 x Analytical.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Narrow Bore. x Narrow Bore RR. x Guard Cartridge, /pk.6 x Guard Cartridge, /pk. x High Performance ZORBAX Guard Fittings Kit *Rx-C8 is the same product as SB-C8. For other sizes and configurations, see ZORBAX StableBond (pg 66) Recommended for alternate selectivity at low ph relative to Eclipse Plus C8, Eclipse XDB-C8 and StableBond SB- C8; for higher temperature applications, StableBond is recommended. Higher carbon load than SB-C8 columns (% vs. 0%) High stability and good peak shape for low ph applications (up to ph 8) Manufactured using dimethyloctadecylsilane and non-endcapped ZORBAX Rx-C8 is the same product as SB-C8 Agilent Pursuit XRs SIZE (mm) PARTICLE SIZE (µm) PURSUIT XRs C8 PURSUIT XRs C8 PURSUIT XRs DIPHENYL.6 x 50 5 A600050X06 A60050X06 A60050X06.6 x 00 5 A600000X06 A60000X06 A60000X06.6 x 50 5 A X06 A600050X06.6 x 50 A60050X06 A60050X06 A6050X06.6 x 00 A60000X06 A6000X06 A6000X06.6 x 50 A600050X06 A60050X06 A60050X06.6 x 0 A60000X06 A6000X06.0 x 50 5 A600050X00 A60050X00 A60050X00.0 x 00 5 A600000X00 A60000X00 A60000X00.0 x 50 A60050X00 A6050X00 A6050X00.0 x 00 A60000X00 A6000X00 A6000X00.0 x 50 A600050X00 A60050X00 A60050X00.0 x 50 5 A600050X00 A60050X00 A60050X00.0 x 00 5 A600000X00 A60000X00.0 x 50 5 A X00 A600050X00 A600050X00.0 x 50 A60050X00 A6050X00.0 x 50 A60050X00 A6050X00 A6050X00.0 x 00 A60000X00 A6000X00 A6000X00.0 x 50 A600050X00 A60050X00 A60050X00.0 x 0 A6000X00.0 x 0 A60000X00.0 x 50 A60050X00.0 x 00 A60000X00 A6000X00 AGILENT PURSUIT XRS COLUMN SPECIFICATIONS BONDED PHASE USP METHOD PORE SIZE p H RANGE END- CAPPED CARBON LOAD Pursuit XRs C8 L 00Å.5-0 Yes % Pursuit XRs C8 L7 00Å.5-0 Yes 5% Pursuit XRs Diphenyl L 00Å Yes.6% Pursuit XRs Ultra C8 00Å.5-0 Yes.% Pursuit XRs Ultra C8 00Å.5-0 Yes 5% Pursuit XRs Ultra Diphenyl 00Å Yes.6% SIZE (mm) PARTICLE SIZE (µm) PURSUIT XRs ULTRA C8 PURSUIT XRs ULTRA C8 PURSUIT XRs ULTRA DIPHENYL.0 x 50.8 A75050X00 A7550X00.0 x 00.8 A75000X00.0 x 50.8 A75050X00.0 x 00.8 A75000X00 A7500X00 A7500X00.0 x 50.8 A750050X00 A75050X00 A75050X00.0 x 0.8 A75000X00 A7500X00 A7500X00 Authorized Distributor

19 HPLC COLUMNS Agilent Polaris Agilent Polaris HPLC Columns In areas like drug discovery where target compounds are increasingly polar, it is critical to have a reversed-phase column that performs well under aqueous conditions. Retention is critical, but cannot come with troublesome secondary interactions. Likewise, phase collapse and shifting retention times need to be avoided. The answer is our Polaris line of polar-modified columns. From the collapse-resistant pore structure of our base silica, to the "wettability" engineered into the bonded phases, Polaris columns have been designed for high aqueous conditions. The combination of high phase density bonding, ultra pure silica, and silanol shielding leads to excellent peak shape among polar-modified columns. As a family, Polaris offers a variety of polar modifications in both C8 and C8 chemistries. AGILENT POLARIS COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE SURFACE AREA CARBON LOAD ENDCAPPED PORE VOLUME LIGAND COVERAGE POLARIS C8-A 80Å 00 m /g.8% Yes. cm /g.9 µmol/m Polaris C8-A 80Å 00 m /g 7.% Yes. cm /g.8 µmol/m Polaris C8-Ether 80Å 00 m /g.% Yes. cm /g. µmol/m Polaris C8-Ether 80Å 00 m /g 7.% Yes. cm /g.5 µmol/m Polaris Amide C8 80Å 00 m /g 5% Yes. cm /g. µmol/m Polaris NH 80Å 00 m /g 5.5% Amide. cm /g.8 µmol/m Polaris Si-A 80Å 00 m /g N/A N/A. cm /g N/A Specifications represent typical values only. Selectivity test mix for Polaris columns 00 mau 0 00 mau 0 Polaris C8-A 0 0 Polaris C8-A min 5 6 min 7 8 VLC0007 Mobile Phase: MeCN water 70:0 Flow Rate:.0 ml/min Temperature: Ambient Detector: UV, 5 nm Polaris HPLC Columns Polaris C8-A Polaris C8-A is the best starting place for separations where the benefits of polar-modified columns are desired. The polar modifications of C8-A help to avoid poor peak shape and retention issues in low organic conditions. Polaris C8-A. Uracil. Caffeine Polaris C8-A offers. an Phenol alternative selectivity to standard. Toluene C8 phases and 5. Butylbenzene has a lower hydrophobicty than Polaris 6. o-terphenyl C8-A, making it ideal for polar samples, 7. Amylbenzene and faster overall 8. analysis Triphenylene times. Polaris C8-Ether Polaris C8-Ether offers an alternative selectivity to Polaris C8-A and standard C8 phases, and typically delivers increased retention of polar compounds away from the void volume. Polaris C8-Ether Polaris C8-Ether offers an alternative selectivity to Polaris C8-A with particular utility for hydrogen bonding compounds. Polaris Amide C8 Polaris Amide C8 has an embedded amide and provides subtle alternative selectivity because they do not have steric protection. LC/MS performance test mix for Polaris C8-A 00 mau 0 0. Aspartame. Cortisone. Reserpine. Dioctyl phthalate min 8 VLC0008 Column: Mobile Phase: Flow Rate: Gradient: Temperature: Detector: Polaris C8-A A000X00.0 x 0 mm, µm A: Water+0.05% HCOOH B: MeCN+0.05% HCOOH 0.6 ml/min 5-90% B in min and hold for min Ambient UV, 0 nm 76 Chrom Tech your chromatography specialists

20 Agilent Polaris HPLC COLUMNS SIZE PARTICLE POLARIS POLARIS POLARIS POLARIS POLARIS POLARIS POLARIS DESCRIPTION (mm) SIZE (µm) C8-A C8-A C8-ETHER C8-ETHER AMIDE C8 NH* Si-A* Column 50.0 x 50 0 A0050X500 Column. x 50 0 A0050X A0050X Column 0.0 x 50 0 A00850X00 Column.6 x 50 0 A0050X06 A0050X06 Metaguard.6 0 A00MG A00MG Column. x 50 5 A00050X A0050X A0050X A050X A0050X Column. x 50 5 A00050X A0050X06 Column. x 00 5 A00000X Column. x 50 5 A00050X Column 0.0 x 50 5 A00050X00 A0050X00 A0050X00 A00650X00 A050X00 Column.6 x 50 5 A00050X06 A0050X06 A0050X06 A00650X06 A050X06 Column.6 x 00 5 A00000X06 Column.6 x 50 5 A00050X06 A0050X06 A0050X06 A0050X06 A00650X06 A050X06 A0050X06 Column.6 x 00 5 A00000X06 A0000X06 A000X06 A0000X06 Column.6 x 50 5 A000050X06 A00050X06 A006050X06 A0050X06 A00050X06 Column.6 x 0 5 A00000X06 Column.0 x 50 5 A00050X00 A0050X00 A0050X00 A0050X00 A0050X00 Column.0 x 50 5 A00050X00 A0050X00 A0050X00 A0050X00 A050X00 A0050X00 Column.0 x 5 5 A0005X00 A005X00 A005X00 A005X00 A05X00 A005X00 Column.0 x 50 5 A00050X00 A0050X00 A0050X00 A0050X00 A00650X00 A050X00 A00550X06 Column.0 x 50 5 A00050X00 A0050X00 A0050X00 A0050X00 A00650X00 A050X00 A0050X00 Column.0 x 00 5 A00000X00 A0000X00 A0000X00 A0000X00 A00600X00 A000X00 A0000X00 Column.0 x 50 5 A000050X00 A00050X00 Metaguard.6 5 A000MG A00MG A00MG A00MG A006MG A0MG A00MG Column.0 x 50 5 A00050X00 A0050X00 A0050X00 A00650X00 A050X00 A0050X00 Column.0 x 50 5 A00050X00 A0050X00 A0050X00 A0050X00 A00650X00 A050X00 A0050X00 Column.0 x 00 5 A00000X00 A00600X00 A000X00 A0000X00 Column.0 x 50 5 A000050X00 A00050X00 A00050X00 A00050X00 A006050X00 A0050X00 Column.0 x 0 5 A00000X00 A00600X00 A000X00 A0000X00 Column.0 x 0 5 A00000X00 A000X00 A0000X00 Metaguard.0 5 A000MG A00MG A00MG A006MG A0MG A00MG Column 0.0 x 50 A0050X00 Column.6 x 50 A0050X06 A050X06 A050X06 A00750X06 A050X06 A00550X06 Column.6 x 50 A0050X06 A050X06 A00750X06 A050X06 A00550X06 Column.6 x 00 A0000X06 A000X06 A00700X06 A000X06 A00500X06 Column.6 x 75 A00075X06 A0075X06 Column.6 x 50 A00050X06 A0050X06 A0050X06 A007050X06 A0050X06 A005050X06 Column.6 x 0 A0000X06 Column.0 x 50 A0050X00 A00750X00 A050X00 A0050X00 Column.0 x 50 A0050X00 A050X00 A00750X00 A050X00 A00550X00 Column.0 x 00 A0000X00 A00700X00 A000X00 A00500X00 Column.0 x 50 A00050X00 A0050X00 A0050X00 A007050X00 A0050X00 A005050X00 Column.0 x 0 A0000X00 Metaguard.6 A00MG A0MG A0MG A007MG A0MG A005MG Column.0 x 50 A0050X00 A050X00 A050X00 A050X00 A00750X00 A050X00 A00550X00 Column.0 x 50 A0050X00 A050X00 A050X00 A050X00 A00750X00 A050X00 A00550X00 Column.0 x 00 A0000X00 A000X00 A000X00 A00700X00 A000X00 A00500X00 Column.0 x 75 A0075X00 Column.0 x 50 A00050X00 A0050X00 A0050X00 A0050X00 A007050X00 A0050X00 A005050X00 Column.0 x 0 A0000X00 A0050X00 A000X00 A00500X00 Column.0 x 0 A0000X00 A000X00 A00500X00 Metaguard.0 A0MG A0MG A0MG A0MG A007MG A0MG A005MG *Normal phase columns Authorized Distributor

21 HPLC COLUMNSAgilent BioHPLC Columns Agilent Poroshell 00 HPLC Columns Separation of Monoclonal Antibody Heavy and Light Chains Column: Gradient: Poroshell 00SB-C8. x 75 mm, 5 µm 5% B, 0.00 min; 0% B, 0.00 min; 5% B, 0.0 min; 5% B,.00 min Mobile Phase: A: H 0-ACN (90:0) B: H 0-ACN (0:90) Flow Rate: Temp: 70 C Detection:.0 ml/min UV 0 nm Chromatographic comparison of antibody IgG after reduction and enzymatic cleavage. 0.5 µm.5 µm Poroshell 00 Particle 5 µm particle 0.5 µm, 00Å porous shell on solid particle StableBond chemistry 5 µm Use Poroshell 00 columns for analyzing intact proteins and large peptide fragments SIZE (mm) 00SB-C8 00SB-C8 00SB-C 00Extend-C8. x x x x.5 Guard Cartridge*, /pk * * * Guard hardware kit x 7 Guard Assembly, /pk *Requires hardware kit AGILENT POROSHELL 00 SPECIFICATIONS BONDED PHASE PORE SIZE TEMP LIMIT p H RANGE END- CAPPED 00SB-C8 00Å 90 C No C8 00Å 90 C No C 00Å 90 C No 00Extend-C8 00Å 60 C 0 C Yes Agilent AdvanceBio RP-mAb Columns Improved accuracy.5 µm superficially porous particle with wide 50Å pore increases mab resolution Selectivity range of phases available (C, SB-C8, Diphenyl) Lifetime/Lower Costs µm inlet frit and robust packed bed extends column lifetime by helping prevent inlet blockage Larger 50Å pore provides less carry-over 0.5 µm.0 µm.5 µm The Agilent AdvanceBio RP-mAb column delivers higher resolution and faster run times to provide accurate, reproducible results when analyzing intact mab, mab fragments (light and heavy chain or Fc and Fab), or other large biomolecules. AdvanceBio RP-mAb Particle SIZE (mm) C SB-C8 DIPHENYL.6 x x x x x x x ADVANCEBIO RP-mAb SPECIFICATIONS BONDED PHASE PORE SIZE TEMP LIMIT p H RANGE END- CAPPED C 50Å 90 C Yes SB-C8 50Å 90 C No Diphenyl 50Å 90 C Yes 78 Chrom Tech your chromatography specialists

22 Agilent BioHPLC Columns HPLC COLUMNS Agilent AdvanceBio Glycan Mapping Columns Speed Obtain glycan maps in less than 0 minutes High-resolution Characterize both labeled and unlabeled glycans; identify any glycosylation changes that occur due to process variables Flexibility Choose from:.7 µm superficially porous particles for high resolution with less backpressure compatible with all LC instruments.8 µm fully porous particles for UHPLC analysis Reproducibility QA tested with a -AB labeled IgG N-linked glycan sample to ensure consistent quality and performance GLYCAN STANDARDS DESCRIPTION PART NO Dextran ladder standard, 0 µg, 0.5 ml vial AB labeled dextran ladder standard, 00 pmol IgG N-linked glycan library, 0 µg, 0.5 ml AB labeled igg N-linked glycan library, 00 pmol ADVANCEBIO GLYCAN MAPPING COLUMNS.8 µm, FULLY POROUS SIZE (mm) MAX PRESSURE PART NO. x bar x bar x 5 Guard, ea 00 bar ADVANCEBIO GLYCAN MAPPING COLUMNS.7 µm, SUPERFICIALLY POROUS. x bar x bar x bar x 5 Guard, ea 600 bar x bar x bar x bar Agilent InfinityLab Poroshell 0 AdvanceBio Peptide Mapping Columns Each batch of AdvanceBio Peptide Mapping media is tested with a rigorous peptide mix to ensure suitability and reproducibility, and to enable the identification of key peptides in NEW! complex peptide maps. 0.5 µm Quality Assurance Testing with Peptide Mix.7 µm.7 µm Column: Flow Rate: Injection: 5 µl Temp: 55 C Detection: Gradient: Sample: AdvanceBio Peptide Mapping x 50 mm,.7 µm 0.5 ml/min 0 nm A, water (0.% TFA) B, ACN (0.08% TFA), 0 5 min, 5 65% B, 5 6 min, 65 95% B Agilent Peptide Mapping Standards Mix (0.5.0 µg/µl per peptide) p/n Test mix used for every batch of AdvanceBio Peptide Mapping media contains 8 hydrophilic, hydrophobic, and basic peptides, ranging in molecular weight from 757 Da to 85 Da. Every column is also tested with a small-molecule probe to ensure efficiency. PK COMPONENT Bradykinin fragment -7 Bradykinin Angiotensin II Neurotensin 5 Angiotensin I 6 ACTH fragment Renin 8 Melittin AdvanceBio Peptide Mapping Columns AGILENT INFINITYLAB POROSHELL 0 ADVANCEBIO PEPTIDE MAPPING COLUMNS SIZE (mm) Poroshell 0 Particle PART NO.6 x x 5 Guard, /pk x x 5 Guard, /pk x x x x 5 Guard, /pk Peptide Standard Authorized Distributor

23 HPLC COLUMNS Agilent BioHPLC Columns Agilent PLRP-S Contain durable and resilient polymer particles that deliver reproducible results over longer lifetimes Thermally and chemically stable Comply with USP L designation Used in bioscience, chemical, clinical research, energy, environmental, food and agriculture, material science and pharmaceutical industries Pore sizes (00Å 000Å) for separations of small molecules to large complexes and polynucleotides The PLRP-S for biomolecules family of products consists of a range of pore sizes and particle sizes, all with identical chemistry and fundamental adsorptive characteristics. The particles are inherently hydrophobic so there is no bonded phase, alkyl ligand required for reversed-phase separations. This gives a highly reproducible material that is free from silanols and heavy metal ions. Within the extensive product range are columns suitable for analytical separations, and preparative purifications. A 0 µm 0 µm 0 µm Scanning electron micrographs (SEM) of PLRP-S 0 µm particles The difference in pore size is clearly seen. A is the small pore 00Å B the larger pore 00Å C the gigaporous 000Å B C SIZE (mm) PARTICLE SIZE (µm) PLRP-S 00Å USP L PLRP-S 00Å USP L PLRP-S 000Å USP L PLRP-S 000Å USP L.6 x 50 8 PL PL5-580 PL x 50 8 PL5-800 PL5-80 PL5-80 PL x 50 8 PL5-80 PL5-80 PL x 50 5 PL PL x 50 5 PL-500 PL x 50 5 PL5-500 PL5-50 PL5-50 PL x 50 PL5-00 PL5-0.6 x 50 PL5-00 PL5-0. x 50 8 PL x 50 8 PL9-80 PL9-80 PL9-80. x 50 8 PL9-80 PL9-80 PL9-80. x 50 5 PL PL x 50 5 PL9-500 PL9-50. x 50 5 PL9-500 PL9-50 PL9-50 PL9-50. x 50 PL9-00 PL9-0. x 50 PL9-00 PL9-0.0 x 50 8 PL-80.0 x 50 5 PL-500 PL-50.0 x 50 PL-00.0 x 50 PL-00 Guard Cartridge,.0 x 5.0 mm, /pk PL6-80 PL6-80 PL6-80 PL6-80 Guard Cartridge holder for.0 x 5.0 mm cartridges PL0-006 PL0-006 PL0-006 PL0-006 PLRP-S APPLICATIONS PORE SIZE 00Å 00Å 000Å 000Å APPLICATION Small molecules/peptides/ oligonucleotides Recombinant peptides/proteins Large proteins DNA/high speed 80 Chrom Tech your chromatography specialists

24 Agilent BioHPLC Columns HPLC COLUMNS Agilent Zorbax 00Å StableBond AGILENT COLUMN SPECIFICATIONS BONDED PHASE PORE SIZE SURFACE AREA TEMP LIMITS* p H RANGE* ENDCAPPED CARBON LOAD ZORBAX 00SB-C8 00Å 5 m /g 90 C No.8% ZORBAX 00SB-C8 00Å 5 m /g 80 C No.5% ZORBAX 00SB-C 00Å 5 m /g 80 C No.% ZORBAX 00SB-CN 00Å 5 m /g 80 C No.% ZORBAX 00-Diphenyl 00Å 5 m /g 80 C Yes.9% Specifications represent typical values only *00StableBond columns are designed for optimal use at low ph. At ph 6-8, highest column stability for all silicabased columns is obtained by operating at temperatures <0 C and using low buffer concentrations in the range of M. At mid or high ph, 00Extend-C8 is recommended O O O OH OH R Si R R Si R R Si R Sterically Protected StableBond Bonded Phase R R R DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) 00SB-C8 USP L 00SB-C8 USP L7 00SB-CN USP L0 00SB-C USP L56 00-DIPHENYL USP L Semi-Preparative 9. x Guard Cartridge, /pk 9. x Guard Hardware Kit Analytical.6 x Analytical.6 x Analytical.6 x Rapid Resolution.6 x Rapid Resolution.6 x Rapid Resolution.6 x Guard Cartridge, /pk.6 x Guard Hardware Kit Solvent Saver Plus.0 x Solvent Saver Plus.0 x Guard Cartridge, /pk.6 x Guard Hardware Kit Narrow Bore. x Narrow Bore. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RR. x Narrow Bore RRHD. x Narrow Bore RRHD. x Guard Cartridge, /pk. x Guard Hardware Kit MicroBore.0 x MicroBore RR.0 x MicroBore RR.0 x MicroBore Guard, /pk.0 x Authorized Distributor

25 HPLC COLUMNSAgilent BioHPLC SEC Agilent AdvanceBio SEC The latest technology for Size Exclusion Chromatography (SEC) analysis of monoclonal antibodies, proteins, and peptides DESCRIPTION 0A 00A ANALYTICAL COLUMNS 7.8 x 00 mm,.7 µm PL PL x 50 mm,.7 µm PL80-50 PL x 00 mm,.7 µm PL PL x 50 mm,.7 µm PL PL580-0 ANALYTICAL GUARDS 7.8 x 50 mm,.7 µm PL80-50 PL x 50 mm,.7 µm PL PL580-0 Agilent AdvanceBio SEC is not recommended for MS applications, see next page for Agilent Bio SEC-. AdvanceBio SEC SPECIFICATIONS PORE SIZE (A ) PARTICLE SIZE (µm) PROTEIN MW RANGE (Da) , ,50,000 AGILENT AdvanceBio SEC PROTEIN STANDARDS DESCRIPTION SIZE PART NO 0A.5 ml vial A.5 ml vial Agilent ProSEC 00S ProSEC 00S columns are designed as a single column solution for globular protein analysis Mechanically robust silica particles that do not bleed during use Extended linear resolving range eliminates the need for pore size selection a single column to analyze most globular proteins Choices to help you perfect your separation Two column IDs to suit multidetector SEC Increased sensitivity when used with light-scattering detectors, to identify dimers, trimers, and aggregates DESCRIPTION 00A ANALYTICAL COLUMNS 7.5 x 600 mm, 5 µm PL x 00 mm, 5 µm PL x 50 mm, 5 µm PL ANALYTICAL GUARDS 7.5 x 50 mm, 5 µm PL x 50 mm, 5 µm PL57-50 ProSec 00S SPECIFICATIONS PORE SIZE PARTICLE SIZE PROTEIN MW RANGE 00Å 5 µm, ,000 Da ph RANGE 7.5 FLOW RATE <.5 (7.5 mm ID) ml/min; <0.5 (.6 mm ID) ml/min TYPICAL OPERATING PRESSURE 50 bar (700 PSI) 8 Chrom Tech your chromatography specialists

26 Agilent BioHPLC SEC HPLC COLUMNS Agilent Bio SEC- Agilent Bio SEC- HPLC columns for protein analysis with MS detection Compatibility with denaturing organic/aqueous mobile phases used for SEC-MS Excellent stability with both high and low-salt conditions Scalability from analytical to preparative workflows DESCRIPTION 00A 50A 00A ANALYTICAL COLUMNS 7.8 x 00 mm, µm x 50 mm, µm x 00 mm, µm x 50 mm, µm ANALYTICAL GUARDS 7.8 x 50 mm, µm x 50 mm, µm PREP COLUMNS. x 00 mm, µm PREP GUARDS. x 50 mm, µm AGILENT Bio SEC- SPECIFICATIONS PORE SIZE (A ) PARTICLE SIZE (µm) PROTEIN MW RANGE (Da) , , ,000,50,000 ph RANGE 8.5 FLOW RATE 0..5 (7.8 mm ID) ml/min; (.6 mm ID) ml/min TYPICAL OPERATING PRESSURE 0 bar (500 PSI) Agilent Bio SEC-5 Agilent Bio SEC-5 HPLC columns are for large biomolecules and samples with multiple molecular weight components Exceptional resolution for large molecules High stability and efficiency due to a proprietary neutral hydrophilic layer Improved peak capacity and resolution due to specially designed packing that increases pore volume Rugged performance Outstanding reproducibility and column lifetime Excellent stability, even under high-ph, high-salt, and low-salt conditions Flexible method development compatible with most aqueous buffers Broad applicability Up to 000Å pore size for vaccines and high molecular weight biomolecules DESCRIPTION 00A 50A 00A 500A 000A 000A ANALYTICAL COLUMNS 7.8 x 00 mm, 5 µm x 50 mm, 5 µm x 00 mm, 5 µm x 50 mm, 5 µm ANALYTICAL GUARDS 7.8 x 50 mm, 5 µm x 50 mm, 5 µm PREP COLUMNS. x 00 mm, 5 µm PREP GUARDS. x 50 mm, 5 µm AGILENT Bio SEC-5 SPECIFICATIONS PORE SIZE (A ) PARTICLE SIZE (µm) PROTEIN MW RANGE (Da) , , ,000,50, ,000,000, ,000 7,500, >0,000,000 ph RANGE 8.5 FLOW RATE 0..5 (7.8 mm ID) ml/min; (.6 mm ID) ml/min TYPICAL OPERATING PRESSURE 0 bar (500 PSI) Authorized Distributor

27 HPLC COLUMNSAgilent GPC/SEC Column Recommendations Recommendations for setting up a GPC/SEC system QUESTION ANSWER RECOMMENDATION COMMENTS CHOOSING AN ELUENT FOR GPC/SEC. What is the sample soluble in? Many polymers are only soluble in a small number of solvents. This is the key question when developing methods for analyzing polymers. The solvents mentioned here are all common eluents employed in GPC/SEC Water or water buffer with up to 50% methanol Organic/water mixtures or polar organics such as, DMF, NMP Typical organic solvent such as THF, chloroform, toluene CHOOSING A COLUMN FOR AQUEOUS/MIXED SOLVENT GPC/SEC. What is the expected molecular weight? It may seem strange to ask this question, but in GPC/SEC the resolution of a column is related to the resolving range. Knowing something of the expected molecular weight of a sample helps to choose the best column that will give optimum results. CHOOSING A COLUMN FOR ORGANIC GPC/SEC. What is the expected molecular weight? It may seem strange to ask this question, but in GPC/SEC the resolution of a column is related to the resolving range. Knowing something of the expected molecular weight of a sample helps to choose the best column that will give optimum results. Columns shown in bold are the best initial choice. SETTING UP THE GPC/SEC SYSTEM. How many columns to use? The greater the particle size of the media in the column (which is dependent on the expected molecular weight of the samples), the lower the resolution and the more columns are required to maintain the quality of the results.. What size injection volume? The injection volume required is dependent on the particle size of the column. Smaller particle sizes require smaller loops to minimize band broadening High (up to several millions) Intermediate (up to hundreds of thousands) Low (up to tens of thousands) Very low (a few thousand) Unknown High (up to several millions) Intermediate (up to hundreds of thousands) Low (up to tens of thousands) Very low (a few thousand) Agilent PL aquagel-oh Agilent PolarGel Agilent PLgel or Agilent PlusPore Aqueous solvents: PL aquagel-oh MIXED-H 8 µm or combination of PL aquagel-oh 0 and 60 5 µm Aqueous solvents: PL aquagel-oh MIXED-M 8 µm Mixed solvents: PolarGel-M Aqueous solvents: Combination of PL aquagel-oh 0 and PL aquagel-oh 0 8 µm Mixed solvents: PolarGel-L Aqueous solvents: PL aquagel-oh 0 5 µm Mixed solvents: PLgel Aqueous solvents: PL aquagel-oh MIXED-M 8 µm Mixed solvents: PolarGel-M PLgel 0 µm MIXED-B or PLgel 0 µm MIXED-A PLgel 5 µm MIXED-C or PLgel 5 µm MIXED-D, PolyPore or ResiPore PLgel µm MIXED-E or MesoPore OligoPore or PLgel µm 00A Best choice for water-based applications but cannot accommodate organics apart from methanol up to 50% PolarGel is a smaller column range than PLgel or PL aquagel-oh columns but is suited to mixtures of organics and water PLgel are the workhorse columns, PlusPore columns are an alternative The 5 µm column combination is best only where sample viscosity is very high, otherwise 8 µm columns give greater resolution A wide-ranging column that covers most watersoluble polymers Covers most applications These two columns in a combined set cover the low end of the molecular weight range For low molecular weight applications This high-performance column give high resolution at low molecular weight No PolarGel column covers this range so use PLgel columns as alternatives Covers the molecular weight ranges of ww polymer samples Covers the majority of applications The PLgel MIXED-A column resolves higher than the PLgel MIXED-B but at lower efficiency due to larger particle size The PLgel columns are the most widely applicable for the majority of applications; PolyPore and ResiPore columns are alternatives The PLgel column provides high resolution and is designed for low molecular weight applications The OligoPore column offers the best possible oligomer separation, PLgel also works well Unknown PLgel 5 µm MIXED-C or PolyPore This PLgel column is the most widely applicable for the majority of applications Depends on the particle size of the columns Depends on the particle size of the columns Particle size 0 µm use columns Particle size µm use columns Particle size 0 µm use columns Particle size 8 µm use columns Particle size 5 µm use columns Particle size µm use columns Particle size 0 µm 00 µl injection Particle size µm 00 µl injection Particle size 0 µm 00 µl injection Particle size 5 µm µl inj. Particle size µm 0 µl injection Increased number of columns required for large particle sizes to make up for low efficiencies. For higher molecular weight samples, larger particles are necessary to reduce the danger of shear degradation of samples during analysis. Smaller particles need lower injection volumes to minimize dead volume. Larger injection volumes allow the introduction of high molecular weight samples at lower concentrations, reducing viscosity and ensuring a quality chromatogram is obtained 8 Chrom Tech your chromatography specialists

28 Agilent Aqueous & Polar GPC/SEC HPLC COLUMNS Agilent PL aquagel-oh SEC columns Highly stable matrix ensures reliable separations even with modified eluents MIXED columns cover a wide spread of molecular weights, simplifying column selection Highly versatile for neutral, polar, anionic and cationic samples Polymer Chemistry Neutral Water soluble polymer Anionic Cationic TECH TIP When using a set of columns containing different pore sizes, put the highest pore size first to reduce the viscosity in the system as early as possible to improve column lifetimes Suggested Eluent Hydrophobic Polymers Pure water 0.-0.M NaNO ph M NaH PO Addition of up to 50% methanol Pure water Guide to eluent selection for PL aquagel-oh applications AGILENT PL AQUAGEL-OH COLUMN SELECTION GUIDE SAMPLE TYPE TYPICAL APPLICATIONS RECOMMENDED COLUMN SETS Low MW polymers and oligomers Polydisperse synthetic or naturally occurring polymers Surfactants, oligosaccharides, PEGs, lignosulfonates, polyacrylates Polysaccharides, PVA, cellulose derivatives, PEO, polyacrylic acid Set of or PL aquagel-oh 0/0 PL aquagel-oh 8 µm, or PL aquagel-oh 0 5 µm, or PL aquagel-oh MIXED-M 8 µm or PL aquagel-oh MIXED-H 8 µm, or PL aquagel-oh 60/50/0 8 µm Very high MW polymers Polyacrylamides, hyaluronic acids, CMC, starches, gums PL aquagel-oh 60/50/0 5 µm in series AGILENT PL AQUAGEL-OH COLUMNS PART NO DESCRIPTION PARTICLE SIZE (µm) MW RANGE (g/mol) (PEG/PEO) EFFICIENCY (p/m) PL AQUAGEL-OH COLUMNS 7.5 x 00 mm PL0-650 PL aquagel-oh ,000 >55,000 PL0-680 PL aquagel-oh ,000 >5,000 PL9-680 PL aquagel-oh 0 8 0,000 00,000 >5,000 PL9-60 PL aquagel-oh 0 5 0,000 00,000 >5,000 PL PL aquagel-oh , ,000 >5,000 PL9-650 PL aquagel-oh , ,000 5,000 PL PL aquagel-oh ,000 0,000,000 >5,000 PL9-660 PL aquagel-oh ,000 0,000,000 >5,000 PL PL aquagel-oh Mixed-H 8 6,000 0,000,000 >5,000 PL9-680 PL aquagel-oh Mixed-M 8, ,000 >5,000 PL AQUAGEL-OH PREPARATIVE COLUMNS 8 µm, 5 x 00 mm PL0-60 PL aquagel-oh ,000 PL9-60 PL aquagel-oh 0 8 0,000 00,000 PL9-650 PL aquagel-oh , ,000 PL9-600 PL aquagel-oh MIXED 8 6,000 0,000,000 PL9-0 PL aquagel-oh Guard, 5 x 5 mm 8 PL AQUAGEL-OH GUARD COLUMNS PART NO DESCRIPTION PARTICLE SIZE (µm) ID (mm) LENGTH (mm) PL9-0 Pl aquagel-oh Guard PL9-50 Pl aquagel-oh Guard PL9-80 Pl aquagel-oh Guard Authorized Distributor

29 HPLC COLUMNSAgilent Aqueous & Polar GPC/SEC Agilent PolarGel GPC Columns Agilent PolarGel-L GPC Columns For analysis of low molecular weight polymers in polar solvents Specifically designed for polar samples Mixed bed technology delivers near linear calibrations for greater accuracy Mechanical stability for longer column lifetimes Agilent PolarGel-M GPC Columns For analyzing a wide range of polymers in polar solvents Two Samples of melamine resin analyzed by PolarGel-L Column: x Polar-Gel-L 7.5 x 00 mm Eluent: Dimethylacetamide + 0.% LiBr Flow Rate:.0 ml/min Inj Vol: 00 µl Temp: 50 C Detector PL-GPC 50 (RI) Optimized pore size distribution and particle size for superior resolution Mixed bed technology simplifies column selection No sample and stationary phase interaction for accurate MW measurement AGILENT POLARGEL GPC COLUMNS PART NO DESCRIPTION POLARGEL-L GPC COLUMNS PL7-680 PolarGel-L, 7.5 x 00 mm PL0-000 Frit Removal Tool for Threaded Columns only PL0-000 End Fitting for Threaded Columns, 7.5 mm ID PL0-00 Frit (5 µm) Kit for Threaded Columns, 7.5 mm ID, 5/pk PL Column Connecting Nuts, /6", 5/pk PL Tubing Ferrules, /6", 5/pk PL0-000 Column End Plugs, /6", 0/pk PL LDV Intercolumn SS Connector PL7-080 PolarGel-L Repair Gel PL7-80 PolarGel-L Guard Column, 7.5 x 50 mm POLARGEL-M GPC COLUMNS PL PolarGel-M, 7.5 x 00 mm PL0-000 Frit Removal Tool for Threaded Columns only PL0-000 End Fitting for Threaded Columns, 7.5 mm ID PL0-00 Frit (5 µm) Kit for Threaded Columns, 7.5 mm ID, 5/pk PL Column Connecting Nuts, /6", 5/pk PL Tubing Ferrules, /6", 5/pk PL0-000 Column End Plugs, /6", 0/pk PL LDV Intercolumn SS Connector PL PolarGel-M Repair Gel PL7-800 PolarGel-M Guard Column, 7.5 x 50 mm Excellent separation of two phenol formaldehyde resins with PolarGel-M Column: x Polar-Gel-M 7.5 x 00 mm Eluent: 0.% (w/v) DMF & 0.% LiBr to reduce sample aggregation Flow Rate:.0 ml/min Inj Vol: 00 µl Temp: 50 C Detector PL-GPC 50 (RI) 86 Chrom Tech your chromatography specialists

30 Agilent Aqueous & Polar GPC/SEC HPLC COLUMNS Agilent PL Rapide Fast separations for high turnaround or when analyzing many samples Analysis of water-soluble polymers in less than ten minutes saves time Significantly increased sample throughput improves efficiency Reduced solvent consumption and disposal costs saves money AGILENT PL RAPIDE COLUMNS PART NO DESCRIPTION SIZE (mm) MW RANGE (g/mol) PL9-800 PL Rapide Aqua H 7.5 x 50 6,000 0,000,000 >5,000 PL PL Rapide Aqua H 0 x 00 6,000 0,000,000 >5,000 PL0-80 PL Rapide Aqua L 7.5 x ,000 >5,000 PL00-80 PL Rapide Aqua L 0 x ,000 >5,000 TYPICAL EFFICIENCY (p/m) Sodium Acrylate Column: PL Rapide Aqua H 7.5 x 50 mm Eluent: Water + 0. M NaNO, 0.0 M NaH PO, ph7 Flow Rate:.0 ml/min Detector: PL-GPC 50 Agilent Polyethylene Glycol/Oxide Standards Simple-to-use kit form Combines glycols and oxides to extend the MW range and cover more applications MWs selected to provide equidistant calibration points for greater accuracy These hydrophobic polymers are suitable for both aqueous SEC and organic GPC using the majority of polar organic solvents. The oxides are available in high molecular weights, while the glycols cover the lower molecular weight range. The two types are chemically similar so they can be used together across a wider molecular weight range, with aqueous and organic polymers from 06- million MW. Every kit contains 0. g or 0.5 g of ten different molecular weight standards. AGILENT CALIBRATION KITS PEG-0 (0 x 0.5 g) PART NO PL CONSTITUENT POLYMER NOMINAL MP (g/mol) 06 0, , , ,000,000 00,000,000 00,000,000 00,000 7,000 00,000, ,000 0,000,000,000 PEO-0 (0 x 0. g) PART NO PL INDIVIDUAL POLYMER MOLECULAR WEIGHTS PART NO ( g) POLYETHYLENE GLYCOL POLYMER NOMINAL Mp (g/mol) PL PL PL PL PL PL PL ,000.0 PL ,500.0 PL ,000.0 PL ,000.0 PL , PL07-000, PL , POLYETHYLENE OXIDE PL , PL , PL , PL , PL , PL , PL , PL , PL , PL , PL08-00,000,000. PL08-00,500,000. NOMINAL Mw/Mn Authorized Distributor

31 HPLC COLUMNSAgilent Organic GPC/SEC Agilent PLgel MIXED Columns CHARACTERISTICS PLgel 0 µm MIXED-A PLgel 0 µm MIXED-B PLgel 5 µm MIXED-C PLgel 5 µm MIXED-D PLgel µm MIXED-E Linear MW Operating Range (g/mol) Typical Column Efficiency (plates/meter) Typical Pressure Maximum Flow Rate SIZE,000 0,000,000 (PS equiv) 500 0,000,000 (PS equiv) PLGEL 0 µ m MIXED-A 00,000,000 (PS equiv) PLGEL 0 µm MIXED-B PLGEL 5 µm MIXED-C 00,00,000 (PS equiv) PLGEL 5 µ m MIXED-D up 5,000 (PS equiv) >8,000 p/m >5,000 p/m >50,000 p/m >50,000 p/m 7.5 x 00 mm: >80,000 p/m*.6 x 50 mm: >70,000 p/m* ml/min (7.5 mm ID): bar (PSI) per 00 mm 0. ml/min (.6 mm ID):. bar (5 PSI) per 50 mm 0 C, 0 C 7.5 mm ID:.5 ml/min.6 mm ID: 0.5 ml/min ml/min (7.5 mm ID): 0 bar (5 PSI) per 00 mm 0. ml/min (.6 mm ID): 8 bar (6 PSI) per 0 mm 0 C, 0 C) 7.5 mm ID:.5 ml/min.6 mm ID: 0.5 ml/min ml/min (7.5 mm ID): 0 bar (5 PSI) per 00 mm 0. ml/min (.6 mm ID): bar (8 PSI) per 50 mm 0 C, 0 C) 7.5 mm ID:.5 ml/min.6 mm ID: 0.5 ml/min ml/min (7.5 mm ID): 0 bar (5 PSI) per 00 mm 0. ml/min (.6 mm ID): bar (8 PSI) per 50 mm 0 C, 0 C) 7.5 mm ID:.5 ml/min.6 mm ID: 0.5 ml/min ml/min (7.5 mm ID): 50 bar (75 PSI) per 00 mm 0. ml/min (.6 mm ID): bar (609 PSI) per 50 mm 0 C) 7.5 mm ID:.5 ml/min.6 mm ID: 0.5 ml/min Maximum Pressure 50 bar (75 PSI) 50 bar (75 PSI) 50 bar (75 PSI) 50 bar (75 PSI) 80 bar (6 PSI) Maximum Temperature 0 C 0 C 50 C 50 C 0 C Recommended No. of Columns/Set Recommended Calibrants x 50 mm or x 00 mm EasiVial PS-H for convenient point calibration in just three injections EasiCal PS- or S-H-0 Kit provides rapid 0 point calibration S-H-0 plus S-M-0 Kits for accurate 9 point calibration See publication EN, GPC/SEC Standards Product Guide x 50 mm or x 00 mm The EasiVial PS-H for convenient point calibration in just three injections EasiCal PS- or S-H-0 Kit provides rapid 0 point calibration Polystyrene S-H-0 plus S-M-0 Kits for accurate 9 point calibration See publication EN, GPC/SEC Standards Product Guide See also: Plgel MiniMIX-B Narrow Bore Columns, reduce the need for expensive solvents x 50 mm or x 00 mm The EasiVial PS-H for convenient 0 point calibration in just three injections EasiCal PS- provides rapid 0 point calibration Polystyrene Kit S-M-0 for accurate 0 point calibration Polyethylene Oxide/ Glycol PEO/PEG-0 Kits for DMF, chemically similar for a broad MW rang See publication EN, GPC/SEC Standards Product Guide *Highest efficiency/resolution achieved only on high performance, low dead volume equipment. x 50 mm or x 00 mm The EasiVial PS-M for convenient point calibration in just three injections EasiCal PS- provides rapid 0 point calibration Polystyrene Kit S-M-0 for accurate 0 point calibration Polyethylene Oxide/ Glycol PEO/PEG-0 Kits for DMF, chemically similar for a broad MW range See publication EN, GPC/SEC Standards Product Guide. x 50 mm or - x 00 mm Polystyrene Kit S-L-0 for accurate 0 point calibration Polyethylene Glycol Kit PEG-0 for DMF, for low molecular weights See publication EN, GPC/SEC Standards Product Guide PLGEL µ m MIXED-E 7.5 x 00 mm PL0-600 PL0-600 PL PL0-650 PL x 50 mm PL PL PL PL PL Guard, 7.5 x 50 mm PL0-0 PL0-0 PL0-50 PL0-50 PL0-0 PMiniMIX Guard,.6 x 50 mm PL50-00 PL50-00 PL PL50-50 PL50-00 PLgel MIXED Gel Calibration Curves PLgel MIXED gel column selection guide 0 µm MIXED-A 0 µm MIXED-B 5 µm MIXED-C 5 µm MIXED-D µm MIXED-E UHMW polymer distributions High MW polymers, demanding eluents Mid range MW polymers, high resolution Resins, condensation polymers Low MW resins, prepolymers PLgel 0 µm MIXED-A PLgel 0 µm MIXED-B PLgel 5 µm MIXED-C PLgel 5 µm MIXED-D PLgel µm MIXED-E Chrom Tech your chromatography specialists

32 Agilent Organic GPC/SEC HPLC COLUMNS Agilent PLgel MIXED LS Eliminates particle leakage to improve data quality with light scattering detection AGILENT PLgel MIXED-LS COLUMNS PART NO DESCRIPTION SIZE LINEAR MW OPERATING RANGE (g/mol) (PS) PL0-600LS PLgel 0 µm MIXED-B LS, 7.5 x 00 mm 500 0,000,000 >5,000 PL0-600LS PLgel 0 µm MIXED-A LS 7.5 x 00 mm,000 0,000,000 >8,000 PL0-0 PLgel 0 µm Guard 7.5 x 50 mm PL0-0 Plgel 0 µm Guard 7.5 x 50 mm TYPICAL EFFICIENCY (p/m) Agilent PLgel MiniMIX Narrow Bore Use about 70% less solvent and save money Store less solvent and increase operator safety High performance comparable to Agilent's conventional ID columns AGILENT PLgel MINIMIX NARROW BORE.6 X 50 mm PART NO DESCRIPTION LINEAR MW OPERATING RANGE (g/mol) (PS) PL PLgel 0 µm MiniMIX-A,000 0,000,000 >7,000 PL PLgel 0 µm MiniMIX-B 500 0,000,000 >5,000 PL PLgel 5 µm MiniMIX-C 00,000,000 >50,000 PL Plgel 5 µm MiniMIX-D 00 00,000 >50,000 TYPICAL EFFICIENCY (p/m) TECH TIP To maintain the same linear velocity through the columns, the volumetric flow rate must be reduced to 0. ml/min in line with the column cross section area, resulting in significantly lower solvent consumption. Sample loading should also be scaled down in line with reduced column volume, and system dead volume should be minimized to avoid excessive band broadening. Reduce the size of peaks when using a refractive index detector by preparing the samples in the eluent that is flowing in the system. Agilent PLgel Olexis Columns Analyzing polymers of very high molecular weight Optimized design for polyolefin analysis High temperature capability High resolution with no damage from sample shear provides clean separations AGILENT PLGEL OLEXIS COLUMNS PART NO DESCRIPTION SIZE PL0-600 PLgel Olexis 7.5 x 00 mm PL0-00 PLgel Olexis Guard 7.5 x 50 mm TECH TIP Remember to heat and cool columns for high temperature analysis slowly to avoid damage from thermal shock. Authorized Distributor

33 HPLC COLUMNSAgilent Organic GPC/SEC Agilent PLgel Individual Pore Size & Preparative Columns Typical applications PLgel µm: Triglycerides, linear hydrocarbons PLgel 5 µm: Acrylates PLgel 0 µm: Rubbers SELECT YOUR PLGEL PREPARATIVE COLUMN COLUMN ID (mm) COLUMN VOLUME PER 00 mm LENGTH (ml) PLgel 7.5 Analytical x PLgel 5 Preparative 7 x MINIMUM SCALE UP Calibration Curves Calibrant: Polystyrene Eluent: TFH Flow Rate:.0 ml/min AGILENT PLGEL INDIVIDUAL PORE SIZE & PREPARATIVE COLUMNS PART NO DESCRIPTION PORE SIZE (A ) PLgel INDIVIDUAL PORE SIZE COLUMNS 7.5 X 00 mm MW RANGE (g/mol) (PS) PL0-60 PLgel µm 00 up to 5,000 >00,000 PL0-0 PLgel µm Guard PL0-655 PLgel 5 µm 50 up to,500 >60,000 PL0-650 PLgel 5 µm 00 up to 5,000 >60,000 PL0-655 PLgel 5 µm ,000 >60,000 PL0-650 PLgel 5 µm ,000 >50,000 PL0-650 PLgel 5 µm 0 0,000 50,000 >50,000 PL PLgel 5 µm ,000,700,000 >50,000 PL0-50 PLgel 5 µm Guard PL0-65 PLgel 0 µm 50 up to,500 >5,000 PL0-60 PLgel 0 µm 00 up to 5,000 >5,000 PL0-65 PLgel 0 µm ,000 >5,000 PL0-60 PLgel 0 µm ,000 >5,000 PL0-60 PLgel 0 µm 0 0,000 50,000 >5,000 PL0-650 PLgel 0 µm ,000,700,000 >5,000 PL0-660 PLgel 0 µm ,000 0,000,000 >5,000 PL0-0 PL0-0 PLgel 0 µm Guard PLgel 0 µm Guard PLgel PREPARATIVE COLUMNS 5 X 00 mm PL0-65 PLgel 0 µm 50 up to,500 PL0-60 PLgel 0 µm 00 up to 5,000 PL0-65 PLgel 0 µm ,000 PL0-60 PLgel 0 µm ,000 PL0-60 PLgel 0 µm 0 0, ,000 PL0-650 PLgel 0 µm ,000,700,000 PL0-660 PLgel 0 µm ,000 0,000,000 PL0-600 PLgel 0 µm MIXED-B 500 0,000,000 PL0-60 PLgel 0 µm MIXED-D 00 00,000 PL0-0 PLgel Prep Guard, 5 x 5 mm TYPICAL EFFICIENCY (p/m) Fractionation of an Oil Distillate Column: PLgel 0 µm 500 Å 5 x 00 mm Sample Cone: 00 mg/ml, ml Eluent: Dichloromethane Flow Rate: 9.0 ml/min Loading: 00 mg on column Detector: UV, 5 nm 90 Chrom Tech your chromatography specialists

34 Agilent Organic GPC/SEC HPLC COLUMNS Agilent HFIPgel Columns Improved performance when using HFIP Optimized separation range delivers high performance with no artifacts Highly durable packing prolongs column lifetime Low operating pressure reduces system wear and unnecessary downtimes Hexafluoroisopropanol (HFIP) is used as a solvent in GPC for the analysis of important industrial polymers such as polyesters, polyamides and polylactide/ glycolide copolymers. For greatly improved performance in extremely polar solvents such as HFIP and trifluoroethanol, Agilent has developed novel "multipore" technology to produce PL HFIPgel, a PS/DVB packing featuring a monodisperse particle size, high pore volume and high resolution. Using PL HFIPgel avoids issues associated with conventional packing and HFIP, such as excessive curvature of calibration curves, dislocations/shoulders on peaks for polydisperse samples and poor resolution in the low MW region. Column efficiency is typically >0,000 plates/meter and the columns are very durable, with a maximum operating pressure of 5 bar (00 PSI). They are packed and tested in methanol, but shipped ready to use in HFIP. PL HFIPgel columns with 7.5 mm ID normally operate at ml/min. However, the.6 mm ID columns run at 0. ml/min, providing a 70% reduction in solvent consumption with savings in the cost of buying and disposing of solvents. AGILENT HFIPgel COLUMNS PART NO DESCRIPTION SIZE PL5-5900HFIP PL HFIPgel.6 x 50 mm PL-6900HFIP PL HFIPgel 7.5 x 00 mm PL-900HFIP PL HFIPgel Guard 7.5 x 50 mm PL5-900HFIP PL HFIPgel Guard.6 x 50 mm Polyamides Column: x PL HFIPgel 7.5 x 00 mm Eluent: HFIP + 0mM NaTFAc Flow Rate:.0 ml/min Temp: 0 C Detector: PL-GPC 50 (RI) TECH TIP Sharp peaks at the front end of GPC/SEC chromatograms (high molecular weight), indicate the sample may be excluding and a column set with a higher resolving range may be required. Agilent PL Rapide Columns Fast separations for high turnaround or when analyzing many samples. Rapid GPC is an excellent tool for screening polymer MWD for trend analysis. Short PL Rapide columns reduce analysis times while maintaining the excellent solvent compatibility and mechanical stability of all GPC columns from Agilent. PL Rapide columns are ideal for high speed applications such as high throughput screening, process monitoring, or tracking changes in MW distributions, where time is the most critical factor in the analysis. PL Rapide is available in low (L), medium (M) and high (H) molecular weights. The F version is for flow injection analysis. Resin Analysis by Rapid GPC Column: PL Rapide L 0 x 00 mm Sample: Epoxy resin Eluent: THF Flow Rate:.0,.0 and.0 ml/min Detector: UV, 5 nm AGILENT PL RAPIDE COLUMNS PART NO DESCRIPTION SIZE (mm) MW RANGE (g/mol) TYPICAL EFFICIENCY (p/m) PL-00 PL Rapide H 7.5 x ,000,000 >5,000 PL0-00 PL Rapide H 0 x ,000,000 >5,000 PL-500 PL Rapide M 7.5 x 50 00,000,000 >60,000 PL0-500 PL Rapide M 0 x 00 00,000,000 >60,000 PL-00 PL Rapide L 7.5 x ,000 >80,000 PL0-00 PL Rapide L 0 x ,000 >80,000 PL-0 PL Rapide F 7.5 x 50 up to,00 >55,000 PL0-0 PL Rapide F 0 x 00 up to,00 >0,000 Authorized Distributor

35 HPLC COLUMNSAgilent Organic GPC/SEC Agilent PlusPore Columns Typical applications for the PlusPore range: PolyPore for the routine analysis of general polymers ResiPore for high resolutions of resins and condensation polymers MesoPore for unsurpassed separation of prepolymers and low MW resins OligoPore for excellent resolution of oligomeric samples AGILENT PLUSPORE SELECTION GUIDE COLUMN MW RANGE (g/mol) (PS) PolyPore 00,000,000 NOMINAL PARTICLE SIZE (µm) TYPICAL EFFICIENCY (p/m) RECOMMENDED CALIBRANTS* 5 >60,000 EasiCal PS- or EasiVial PS-H ResiPore up to 500,000 >80,000 EasiCal PS- or EasiVial PS-M MesoPore up to 5,000 >80,000 Polystyrene S-L-0 Kit OligoPore up to,00 6 >55,000 Polystyrene S-L-0 Kit *See page 9 for recommendations SIZE (mm) POLYPORE RESIPORE MESOPORE OLIGOPORE FRIT POROSITY (µm) 5 x 00 PL x 00 PL-6500 PL-600 PL-65 PL x 50 PL PL5-500 PL5-55 PL5-550 Guard,.6 x 50 PL5-500 PL5-00 PL5-0 Guard, 7.5 x 50 PL-500 PL-00 PL-5 PL-0 PlusPore Calibration Curves PolyPore ResiPore MesoPore OligoPore Agilent EnviroPrep Columns Environmental clean up with EPA methods High sample loading ensures effective trace analysis Simple clean-up procedure saves sample preparation costs Optimized particle size distribution provides high resolution EnviroPrep columns permit a simple, one stage clean-up to determine pesticides in many organic matrices. The higher molecular weight fractions such as lipids, polymers, natural resins and dispersed high molecular weight components are easily eliminated in the GPC analysis Columns for Sample Clean-Up Column: EnviroPrep, 5 x 00 mm EnviroPrep, 5 x 50 mm Eluent: DCM Flow Rate: 0 ml/min Detector: UV, 5 nm AGILENT ENVIROPREP COLUMNS SIZE (mm) PART NO. x 50 PLE0-0EPA 5 x 50 PL0-0EPA. x 00 PLE0-60EPA 5 x 00 PL0-60EPA TECH TIP Preparative GPC for soil extract clean-up is described in EPA Method 60A using 5 x 00 mm and 5 x 50 mm columns to give higher sample loading and fraction yields, which is particularly useful for low levels of pollutants. Low pore size EnviroPrep columns are ideal for this method. The columns have 0 µm particles with 00A pore sizes for high resolution, with an exclusion limit of,000 MW. The preparative columns offer good resolution and high loading through optimization of the particle size distribution. 9 Chrom Tech your chromatography specialists

36 Agilent Organic GPC/SEC HPLC COLUMNS Agilent Polystyrene Standards Compatible with most organic solvents Certificate of Analysis meets international protocols Polystyrene standards are the first choice for may organic solvents, either for conventional GPC column calibration or for calibrating light scattering and viscosity detectors. Our organic polymers cover a range from 6-5 million MW, with MWs selected to provide equidistant calibration points for greater accuracy. Every kit contains 0.5 g of ten different molecular weight standards. AGILENT CALIBRATION KITS, (ALL KITS 0 X 0.5 g ) PL00-00 PL00-00 PL PL00-00 PL00-00 PL CONSTITUENT POLYMER NOMINAL MP (g/mol) 00,000, ,000,000,50, ,000 8,600,000, ,00,000 5,000 0,000,750,00 800,700,000 7,000 7,000 9,500,00,000,600,000 0,000 66,000 9,000,00,500,000, ,000 80,000 8,000 5,00,900 6,00,000,780,000 60,000 75,000 8,00,500 9,500,000 5,000,000,90,000 50,000,800,00 5,000,000 5,000,000,000,000 00,000 0,000,500 INDIVIDUAL POLYMER MOLECULAR WEIGHTS POLYMER NOMINAL M p (g/mol) PART NO ( gram) POLYMER NOMINAL M p (g/mol) PART NO ( gram) 6 PL ,000 PL PL ,000 PL PL ,000 PL0-700,000 PL ,000 PL0-800,00 PL ,000 PL0-900,000 PL ,000 PL0-000,000 PL0-600,000,000 PL0-00 5,000 PL0-700,500,000 PL0-00 7,000 PL0-800,000,000 PL0-00 0,000 PL0-900,500,000 PL0-00 0,000 PL0-00,000,000 PL ,000 PL0-00 7,000,000 PL ,000 PL0-00 0,000,000 PL ,000 PL0-00 5,000,000 PL0-900 Agilent EasiVial Polymer Standards Eliminates tedious weighing procedures to improve calibration accuracy Reduces solvent dispensing to limit risks associated with handling solvents For conventional and multi detector GPC to maximize applicability AGILENT EASIVIAL POLYMER STANDARDS VOLUME EasiVial PS-H EasiVial PS-M EasiVial PS-L EasiVial PM EasiVial PEG/PEO EasiVial PEG ml Vials (0/pk) ml Vials (0/pk) PL00-00 PL00-00 PL00-00 PL00-00 PL PL PL PL PL PL PL PL EASY VIAL KIT CONTENTS, NOMINAL Mp ( g/mol) Red Vial Cap Yellow Vial Cap Green Vial Cap, , ,000 6,000,000 0,000,000, ,000 50,000 0,000 00,000 5,000 6,000 6,000,000 00,000 0,000,000,000,00,000 5, , ,500,500,000,000, ,000 5,000 6,000 50,000 60,000,750,000,000 00,000 5, ,000,000,000, ,00,500,000 5,700, ,000,000,000 80,000 5,000, ,000 00,000 6,000 70,000 60,000,000 Easy Vial kits contain three vials, each with a mixture of four accurately pre-weighed polymer standards, providing a -point GPC calibration in just three injections. Description Key PS: POLYSTYRENE PM: POLYMETHYLMETHACRYLATE PEG/PEO: POLYETHYLENE GLYCOL/OXIDE H: HIGH M: MEDIUM L: LOW Authorized Distributor

37 HPLC COLUMNSConcise Separations Carbohydrates Concise Separations CARBOSep Columns for Carbohydrate Analysis Stable at temperatures up to 95 ºC Consistent from column to column, and polymer batch to polymer batch The simplest and safest eluent of all water More choices of columns utilizing combinations of cross-linkage (porosity) particle size, metal ligands and column formats to maximize your separation Ligand exchange is the preferred method for the separation of many sugars and sugar alcohols due to the simple water eluent. In ligand exchange, the negatively charged hydroxyl groups on the carbohydrate molecule interact with the positively charged metal loaded groups on the chromatography substrates. The carbohydrates are eluted by the polar water eluent mobile phase which competes for the sites on the metal ion. Besides the ligand exchange mechanism, several secondary mechanisms processes are also involved in the separation of the carbohydrates including size exclusion and normal phase partitioning. HPLC columns packed with low cross-linked polymers (gels) serve as the primary packings for carbohydrate analysis columns, and are available from a number of suppliers. In order to maximize the separation of a wide variety of samples, Concise Separations has developed the most complete line of carbohydrate analysis columns available on the market by combining ligand exchange (metals), size exclusion and partitioning (cross-linkage of polymer), particle size (column efficiency) and column size (speed versus resolution). Since polymers are chemically stable, as long as the columns are used within the operating parameters, they last a long time. The key to long column lifetime when using polymeric gels to keep the column at all times below the pressure maximum. Since temperature is a key component of pressure along with flow rate, it is extremely important to allow the column to reach temperature before starting the flow. The columns are also sensitive to water quality, so water purity is essential (minimum purity requirements 8 Mohm). Sample preparation as well as the use of guard columns and in-line filters reduce contaminants from entering the column and will extend a column s lifetime. In general, the higher the crosslinkage of the polymer and the larger the particle size, the greater the flow rate that can be used before reaching the maximum allowable pressure. NEW CARBOSep Column Applications Separation of Sugars on CHO-78 column Condition: H O with 0.6 ml/min at 70 ºC RI detection. cel. glu. xyl. gal 5. ara 6. man Biomass Analysis Sugar Alcohols Analysis Retention Time (minutes) Separation of Alcohols on CHO-88 Column Condition: 0.8 ml or 0.6 ml/min with H O at 85 ºC RI detection Flow-rate: 0.8 ml/min Flow-rate: 0.6 ml/min Retention Time (minutes) 6. ery. man. xyl. sor Tips on Maintaining the Performance of Concise Separations Columns The most important fact to remember when using Concise Separations columns is that the polystyrene-divinylbenzene copolymer is a low cross-linked material. This polymeric packing has a limited resistance to flow rate and pressure and will irreversibly compact and overpressure the resin at a certain level. Unlike polymers, silica based materials are not flow rate sensitive and the relation between pressure and flow rate remains relatively constant. Therefore, the columns should be carefully monitored for pressure and should be operated within the recommended flow rates and pressure specifications. Use column ovens to serve the dual purpose of increasing efficiency and lowering back pressure. Set the pressure shut off for the analytical test system at or slightly below the recommended column pressure maximum to prevent irreversible damage. When installing, allow the column to warm up in the column oven for 5 minutes, and then start the flow rate below your target flow rate. After 5 minutes, increase the flow rate to the target flow rate and confirm that the column is operating at the expected back pressure. To increase the lifetime of your analytical column, we recommend the proper use of guard columns or cartridges. How frequently you change your guard column depends on pretreatment or sample purity. Filter and remove potentially harmful organics from the samples to decrease the need to change guard columns. Carefully monitor them for pressure increase and the chromatograms for changes in retention and efficiency to determine the approximate useful lifetime of the guard columns. 9 Chrom Tech your chromatography specialists

38 Concise Separations Carbohydrates HPLC COLUMNS Concise Separations CARBOSep Columns for Carbohydrate Analysis (cont.) Retention Chart Another useful tool in choosing the best column for your sample is the use of retention charts. Compounds with at least one minute difference in retention time should be adequately separated. However, the wide variety of carbohydrates precludes developing a comprehensive chart for all compounds. Also, by using different temperature and flow rates, the selectivity of the column can be altered to enhance the separation of the compounds. If your compound does not appear in a retention chart, or the ability of a column to separate your compounds is in question, please contact Chrom Tech technical support. RETENTION TABLE FOR CARBOSEP COLUMNS COMPOUND CHO-60 COREGel-87N COREGel-87C & CHO-80 COREGel-87P & CHO-88 CHO-68 COREGel-87K Nitrate Maltoheptose Maltohexose Maltotpentose Amiprylose Stachyose Maltotetrose Melezitose Raffinose Maltotriose Cellobiose Trehalose Sucrose Maltose Melibiose Lactose Lactulose Glucose Lactitol Xylose Maltitol Galactose Sorbose Mannose Rhamnose Fructose Fucose Arabinose Myo-inositol Digitoxose Ribitol Tagatose Mannitol Arabitol Xylitol Galactitol Sorbitol Ribose COREGel-87C-8% Condition: 0.6 ml/min with H0 at 85 ºC, RI Detector Resolution and Cross-linkage Effect The lower the cross-linkage, the larger the pore size. For samples containing larger sugar polymers, the industry standard 8% cross-linked polymer may not adequately resolve your sample DP+. DP. mal. glu 5. gal 6. fru 7. man 8. sor CHO-60-6% XL Condition: 0.5 ml/min with H0 at 90 ºC, RI Detector DP5+. DP. DP. mal 5. glu 6. gal 7. fru 8. man 9. sor Authorized Distributor

39 HPLC COLUMNSConcise Separations Carbohydrates Concise Separations CARBOSep Columns for Carbohydrate Analysis (cont.) CONCISE SEPARATIONS CARBOSEP COLUMN COMPARISON CHART PHASE CROSS- LINKAGE IONIC FORM PARTICLE SIZE (µm) KEY SAMPLES COMMENTS CHO- Na Sodium 0 Oligosaccharides through DP0 Easier to regenerate than Ag+ form CHO-6 Na 6 Sodium 0 Oligosaccharides through DP5, reproducible separation of corn syrup Separates by both ligand exchange and size exclusion CHO-6 OH Na 6 Sodium 0 Fast analysis of simple sugars PAD detector compatible CHO-68 Pb 6 Lead 7 High resolution column, including sucrose/maltose/lactose Pressure sensitive, low flow rates CHO-60 Ca 6 Calcium 0 Versatile analysis of corn syrup, sugars, sugar alcohols Concise Separation s most popular carbohydrate column CHO-78 Pb 7 Lead 7 Biomass sugar analysis, great for samples containing carbohydrates and sugar alcohols Excellent selectivity with faster flow rate than the CHO68 CHO-80 Ca 8 Calcium 9 General sugar analysis Higher efficiency version of COREGel 87C USP L9 Ca 8 Calcium 8 Mannitol and Sorbitol - USP approved Very rugged USP column CHO-88 Pb 8 Lead 7 Monosaccharides and cellulose products Higher speed, lower resolution than CHO68 CHO-88 Pb Fast 8 Lead 7 Fast analysis of monosaccharides Quick analysis COREGel 87C Ca 8 Calcium 9 Industry standard for analysis of general sweeteners Compatible replacement for the Bio-Rad Aminex HPX 87C COREGel 87C Fast 8 Calcium 9 Fast analysis of simple sugars Quick analysis with a rugged column COREGel 87MM Ca Na 8 Sodium/ Calcium 8 Fast analysis of sugar alcohols Easily cleaned with EDTACaNa COREGel 87K K 8 Potassium 9 Target application corn syrup and molasses. Sugar samples such as brewing wort, betaine analysis Use with samples containing potassium, Compatible replacement for the Bio-Rad Aminex HPX 87K COREGel 87N Na 8 Sodium 9 Molasses and other sugars high salt samples Easy to regenerate, low selectivity. Compatible replacement for the Bio-Rad Aminex HPX 87N COREGel 87P Pb 8 Lead 9 Monosaccharides and cellulose products Less resolution than CHO88, high flow rate. Compatible replacement for the Bio-Rad Aminex HPX 87P DESCRIPTION SIZE CHO- Na CHO-6 Na Corn Syrup CHO-6 OH Na CHO-68 Pb CHO-60 Ca CHO-78 Pb Carbo & Biomass CHO-80 Ca Column 7.8 x 00 mm CHO CHO CHO CHO Column 7.8 x 00 mm CHO CHO Column 7.8 x 50 mm CHO Column Column (Waters) 6.5 x 00 mm CHO CHO CHO W Column 6.5 x 50 mm CHO Guard Kit ( holder, /pk cartridges) CHO-99-7 CHO-99-5 CHO-99-5 CHO-99-5 CHO-99-5 CHO-99-7 CHO Guard Cartridges (/pk) CHO-99-7 CHO-99-5 CHO-99-5 CHO-99-5 CHO-99-5 CHO-99-7 CHO DESCRIPTION SIZE USP L-9 Ca CHO-88 Pb COREGel-87C Ca COREGel-87MM Ca/Na COREGel-87K COREGel-87N Na COREGel-87P Pb Column 7.8 x 00 mm CHO CHO CHO CHO CHO CHO Column (Fast) 7.8 x 50 mm CHO Column (Fast) 7.8 x 00 mm CHO Column.0 x 50 mm CHO Guard Kit ( holder, /pk cartridges) CHO CHO-99-7 CHO CHO CHO-99-6 CHO-99-6 CHO-99-6 Guard Cartridges (/pk) CHO CHO-99-7 CHO CHO CHO-99-6 CHO-99-6 CHO-99-6 TECH TIP Alltech 700CH The popular Alltech 700CH (P/N: 70057) is the Concise CarboSep CHO 60 Ca (P/N: CHO ) column. 96 Chrom Tech your chromatography specialists

40 Concise Separations Carbohydrates HPLC COLUMNS Concise Separations CARBOSep Columns for Carbohydrate Analysis (cont.) CHO- Sodium 0. ml/min with H0 at 85 ºC, RI Detector. DP0+. DP9. DP8. DP7 5. DP6 6. DP5 7. DP 8. DP 9. mal 0. glu CHO-6 Sodium 0.5 ml/min with H0 at 90 ºC, RI Detector. DP5. DP. DP. mal 5. glu CHO-6 OH Sodium 0.5 ml/min with H0 at 90 ºC, RI Detector. suc. glu. ara 6 CHO-68 Lead 0. ml/min with H0 at 80 ºC, RI Detector. suc. mal. glu. xyl 5. gal 6. ara 7. man CHO-60 Calcium 0.5 ml/min with H0 at 90 ºC, RI Detector. sta. raf 5. suc 6 7. glu 5. gal 6. fru 7. man 8. sor 8 CHO-78 Lead 0.6 ml/min with H0 at 70 ºC, RI Detector. cel. glu. xyl. gal 5. ara 6. man 5 6 CHO-80 Calcium 0.5 ml/min with H0 at 90 ºC, RI Detector. raf. suc. glu. gal 5. fru 6. man 7. sor USP L-9 Calcium 0. ml/min with H0 at 0 ºC, RI Detector. man. sor Fast CHO-88 Lead 0.5 ml/min with H0 at 90 ºC, RI Detector. glu. xyl. ara. man CHO-88 Lead 0.5 ml/min with H0 at 80 ºC, RI Detector. glu. xyl. ara. man Fast COREGel-87C 0.6 ml/min with H0 at 85 ºC, RI Detector. mel. mal. glu. man 5. fru 6. rib 5 6 COREGel-87C 0.6 ml/min with H0 at 85 ºC, RI Detector. mel. mal. glu. man 5. fru 6. rib COREGel-87MM 0.6 ml/min with H0 at 85 ºC, RI Detector. DP+. DP. DP. mal 5. glu 6. gal 7. fru 8. man 9. sor COREGel-87K 0.6 ml/min with H0 at 85 ºC, RI Detector. raf. suc. bet. glu 5. fru 5 COREGel-87N 0.6 ml/min with H0 at 85 ºC, RI Detector. raf. suc. glu. fru COREGel-87P.0 ml/min with H0 at 85 ºC, RI Detector 6 7. cel. glu. xylo 5. gal 5. ara 6. xyli 7. sor Authorized Distributor

41 HPLC COLUMNSConcise Separations Organic Acids Concise Separations Columns for Organic Acid Analysis Stable in the ph range of 0 to Stable at high temperatures up to 90º C Consistent performance through numerous sample injections (depending on sample preparation, instrument maintenance, and the use of guard systems) No need for gradients for sample analysis due to the use of simple dilute acid allowing use of universal detectors such as RI detectors Eliminates the need for high cost solvents (including waste disposal) Eluent serves as a self regenerating cleaning solution and does not degrade the column Retention Chart Another useful tool in choosing the best column for your sample is the use of retention charts of many common organic acids. If your compound does not appear in a retention chart, or the ability of a column to separate your compounds is in question, please contact Chrom Tech technical support. Eluent Effect By controlling the true strength of the acidic eluent, the retention times of the compounds can be influenced. The stronger (more acidic) the eluent, the longer the retention times in relation to the pka. The eluent strength can be used by the analyst to enhance the separation of compounds. Separation of L-Ascorbic and D-Sorbitol species on Columns: 87H and 6H 0.6 ml/min at 80 ºC, RI Detector 87H_5mM 87H_8.0mM. L-Ascorbic Acid. D-Sorbitol RETENTION TABLE FOR ORGANIC ACID COLUMNS COMPOUND COREGEL ION00 COREGEL ORH80 COREGEL 6H COREGEL 07H Malic Malonic cis-aconitic Adipic Formic Maleic Ascorbic Butyric 8.5. Glycolic Glycoxilic Citric Tartaric Nicotinic 6. Propionic Succinic Oxalic Sorbic Acrylic Isobutyric.8. Lactic Shikimic Fumaric Glutaric Pyruvic Acetic Proponal Quinic COREGEL 87H 6H_.5mM 6H_8.0mM Separation of L-Ascorbic and D-Sorbitol on Coregel 87H at Different Temperatures.5 mm H SO at 0.6 ml/min, RI Detector 80 ºC 60 ºC 50 ºC 5 ºC. L-Ascorbic Acid. D-Sorbitol TECH TIP Temperature Effect Temperature is by far the most powerful tool used to influence relative retention of compounds on Concise Separations ion-exclusion columns. By manipulating temperature, in combination with eluent strength and column types (polymer crosslinkage), an analyst can greatly enhance species separation. 98 Chrom Tech your chromatography specialists

42 Concise Separations Organic Acids HPLC COLUMNS CONCISE SEPARATIONS ORGANIC ACID ANALYSIS COLUMN COMPARISON CHART PHASE CROSS- LINKAGE IONIC FORM PARTICLE SIZE (µm) KEY SAMPLES COMMENTS COREGEL-87H 8 H 0 Short bed length allows for fast analysis of simple acid samples COREGEL-87H 8 H 9 Good resolution of many common organic acids High durability COREGEL ION-00 6 H 7 Separates organic acids, alcohols and carbohydrates all on the same column Ruggedness combined with fast analysis Popular column. Select when high resolution is the primary concern COREGEL-07H 0 H 8 Improved resolution for organic acids New higher cross-linked column COREGEL ORH-80 7 H 9 Versatile column for organic acids, alcohols and carbohydrates COREGEL ORH-80FA 7 H 8 Fast analysis for fermentation monitoring, versatile column for organic acids, alcohols and carbohydrates COREGEL WA- Wine Analysis 8 H 8 Rugged design allows for little sample prep High resolution makes the WA excellent for QA COREGEL ION-0 8 H Designed for fast analysis of organic acids and alcohols Popular column. Provides good balance of high efficiency and ruggedness Popular column. Provides good balance of high efficiency and ruggedness Higher efficiency but as robust as the work horse 87H column Ideal for the analysis of borate and bicarbonate COREGEL ARH-60 6 H 8 Designed for the separation of aromatic organic acids Uses aqueous mobile phases COREGEL-6H 6 H 0 Versatile column for organic acids, alcohols and carbohydrates COREGEL USP L-7 8 H 8 Complies with USP L-7 specifications for the separation of citric, lactic, and acetic acid, can also separate a wide number of other organic acids Provides good balance of high efficiency and ruggedness Hydrogen form ion-exclusion column TECH TIP Popular Grace/Alltech Columns The IOA 000 Column, (P/N: 968) is the ION 0 column. The OA 000 Column, (P/N: 908) is the ARH 60 column. The IOA 000 Columns, (P/N: 906) is the ION 00 column. The OA 000 Column, (P/N: 906) is the ORH 80 column. See ordering chart on page 00 Authorized Distributor

43 HPLC COLUMNS Concise Separations Columns for Organic Acid Analysis (cont.) DESCRIPTION SIZE COREGEL-87H COREGEL-87H COREGEL ION-00 COREGEL-07H COREGEL ORH-80 Column 7.8 x 00 mm ICE ICE ICE Column (Fast) 7.8 x 00 mm ICE Column 7.8 x 50 mm ICE Column 7.8 x 00 mm ICE Column 6.5 x 00 mm ICE ICE Column (Fast) 6.5 x 50 mm ICE Guard Kit ( holder, /pk cartridges) ICE ICE-99-6 ICE ICE ICE-99-5 Guard Cartridges (/pk) ICE-99-7 ICE ICE ICE-99-6 DESCRIPTION SIZE COREGEL WA- Wine COREGEL ION-0 COREGEL ARH-60 COREGEL-6H COREGEL USP L-7 Column 7.8 x 00 mm ICE ICE Column (Fast) 6.5 x 00 mm ICE ICE Column. x 50 mm ICE Guard Kit ( holder, /pk cartridges) ICE ICE ICE-99-5 ICE ICE-99-5 Guard Cartridges (/pk) ICE-99-0 ICE ICE-99-6 ICE ICE-99-6 Coregel-87H.0 ml/min with DDI H0 at 5 ºC, RI Detector Coregel-87H 0.6 ml/min with N H SO at 5 ºC, UV 0 Detector Coregel ION ml/min with N H SO at 70 ºC, RI Detector Coregel-07H 0.6 ml/min with N H SO at 5 ºC, UV at 0. cit. mal. suc. form. oxal. cit. tar. mal 5. suc 6. form 7. fum cit. tar. glu. mal 5. fru 9 6. lac 7. gly 8. ace 9. MeOH 0. EtOH 0. cit. alp. fum. ace Coregel-07H FA Coregel ORH-80 Coregel ORH-80 FA Coregel WA- Wine Analysis 0.6 ml/min with 8.0 mm H SO at 80 ºC. glu. for. met. eth 0.6 ml/min with 0.0 N H SO at 5 ºC, RI Detector oxal. cisaco. tar. mal lac 6. form 7. fum 8. prop 9. but 0.6 ml/min with 8.0 mm H SO at 80 ºC, RI Detector. malto 5. suc. maltose 6. lac. glu 7. gly. fru 8. ace 9. eth ml/min with N H SO at 5 ºC, RI Detector. cit 7. lac. tar 8. gly. glu 9. ace. mal 0.,-but 5. fru. impurity 6. suc. eth Coregel ION ml/min with 0.0 N H SO at 50 ºC, RI Detector. suc. glu. gly. ace 5. EtOH 5 Coregel ARH ml/min with 0 mm H SO at 5 ºC, UV 0. cit. shi. fum. but 5. hom 6. gal 7. pro Coregel-6H 0.6 ml/min with N H SO at 5 ºC, UV 0. oxal. cit. tar. mal 5. asco 6. suc 7. form 8. fum Coregel USP L ml/min with DDI H O at 60 ºC, RI Detector. cit. lac. ace Chrom Tech your chromatography specialists

44 HPLC COLUMNS Concise Separations Columns for RNA, Protein & Peptides Stable in the ph range of 0 to Completely stable under high temperature conditions which eliminate or reduce secondary tertiary effects of analytes Chemically stable which permits a variety of cleaning solutions for effective column cleaning Flow reversibility to facilitate the removal of contaminants on the inlet end of the column bed High efficiency, mono-dispersed beads Pure hydrophobic properties provided by proprietary and patented C8 functionality chemistry RPSep PRX ml/min with 80% ACN/0% H O at ambient, UV 5 5. void vol. eth. pro. but 5. pen 6. hex 6 Concise Separations ProteinSep columns offer unique characteristics to provide the protein chemist another valuable tool for the many varieties of samples in the proteomics field. The proprietary and highly rugged polymeric based columns will provide long lasting, reproducible results for a wide variety of samples. The Concise Separations protein analysis columns are packed with durable and high efficiency polymers that perform well even under the most extreme test conditions. CONCISE SEPARATION COLUMNS FOR ANION ANALYSIS (STRONG ANION EXCHANGE COLUMNS) COLUMN RPSep PRX- ProteinSep RiboSep RNA APPLICATION Porous PS/DVB Polymer. Ideal for the separation of peptides and small molecules, ph stable from 0 Non-porous PS-DVB Polymer with C8 Functional Group, monodispersed um bead. High temperatures allowed, ph stable from 0 Polymeric column, excellent for identification and purity assays for RNA fragments that are 00 to > 6,000 nt. Can be used with or without a column oven. DESCRIPTION SIZE RPSep PRX- Protein Sep RiboSep RNA Column 7.8 x 50 mm RPC Column.6 x 50 mm RPC Column.6 x 50 mm RPC Column.6 x 50 mm PRO Guard Cartridges (/pk) RPC-99- ANX ProteinSep. Ribonuclease A. Cytochrome C. Lysozyme. Myoglobin 5. Chymotrypsinogen A ml/min at 80 ºC, Buffer A: CF₃CO₂H in H 0 B: CF₃CO₂H in ACN, 0% - 50% B in 5 min. RiboSep RNA min, Solvent A: 0.M TEA Solvent B: 0.M TEAA, 5% ACN 0 µl. 00 nt. 00 nt. 00 nt. 00 nt nt Authorized Distributor

45 HPLC COLUMNSConcise Separations Amino Acids Concise Separations Columns for Amino Acid Analysis Rugged polymeric substrate, stable in ph range of 0 to High efficiency and resolution Reproducibility lot-to-lot and column-to-column Available for both physiological samples (Li+ format) and protein hydrolysate samples (Na+ form) Post column derivitization detection Ion-exchange chromatography is a popular technique for the analysis of amino acids because both retention times and quantification are highly reproducible regardless of the sample matrix. This unique matrix insensitivity is important when comparing results from different patients or batches of protein hydrolysate. Amino acids are zwitterions; at low ph, they are positively-charged and are bound to the resin by their attraction to the negatively-charged ion-exchange sites. Almost all the contaminants, i.e. matrix, are eluted at the void. The amino acids are then selectively eluted by increasing the ph and salt concentration with different buffers. With few exceptions, the order of elution follows the isoelectric point of the amino acids, i.e. acidic amino acids first, then neutral and basic. Because the separation and the ensuing post-column reaction of amino acids are devoid of contaminants, amino acid analyses via ion-exchange chromatography are highly reproducible. CONCISE SEPARATIONS AMINO ACID ANALYSIS COLUMN COMPARISON CHART PHASE CROSS- LINKAGE IONIC FORM PARTICLE SIZE (µm) KEY SAMPLES COMMENTS AMINOSep AA-9 8 Sodium 9 Designed for complicated samples from protein hydrolysates. Increased polymer bed yields better resolving power AMINOSep AA-5 0 Sodium 5 Designed for faster analysis than the AA-9 but still gives high resolution Lithium Amino Acid (600 & 700 systems) Sodium Amino Acid (600 & 700 systems) Sodium Amino Acid (for System Gold) 0 Lithium 6 Designed for use with the Beckman Coulter 600 and 700 Amino Acid Analyzers using either the Beckman or Pickering Lithium buffer systems 0 Sodium 5 Designed for use with the Beckman Coulter 600 and 700 Amino Acid Analyzers using either the Beckman Coulter or Pickering Sodium buffer systems 0 Sodium 5 Designed for use with the Beckman Coulter System Gold Amino Acid Analyzer Popular column Higher capacity than the Beckman columns Most popular AA column Ideal for Physiological amino acid analysis Ideally suited for routine hydrolysate analysis, Extremely rugged polymer Ideal for the separation of hydrolysate amino acids. 0 Chrom Tech your chromatography specialists

46 Concise Separations Amino Acids HPLC COLUMNS DESCRIPTION SIZE AMINOSep AA-9 AMINOSep AA-5 LITHIUM AMINO ACID (600/700) SODIUM AMINO ACID (600/700) Column.6 x 50 mm AAA Column (Waters).6 x 50 mm AAA W Column.6 x 50 mm AAA Column.6 x 0 mm AAA SODIUM AMINO ACID (System Gold) Column.0 x 00 mm AAA Column.0 x 0 mm AAA-99-6 Column.0 x 00 mm AAA-99-6 Guard Kit ( holder, /pk cartridges) AAA-99-5 AAA-99-5 AAA-99- AAA-99- AAA-99- Guard Cartridges (/pk) AAA-99-5 AAA-99-5 AAA-99- AAA-99- AAA-99- AMINOSep AA-5 Sodium Column 0.5 ml/min with Sodium Citrate A, B, C at 8 ºC, Fluorescence Detector AMINOSep AA-5 High Speed Sodium Column 0.5 ml/min with Sodium Citrate A, B, C at 60 ºC, Fluorescence Detector asp. thr. ser. glu 5. gly 6. ala 7. val 8. met 9. ile 0. leu. tyr. phe. lys. his 5. arg AMINOSep AA-9 Sodium Column 0.5 ml/min with Sodium Citrate A, B, C at 8 ºC, Fluorescence Detector asp. thr. ser. glu 5. gly 6. ala 7. val 8. met ile 0. leu. tyr. phe. lys. his 5. arg Sodium Amino Acid Column (System Gold) 6 ml/hr with NaE, NaF, NaD at C, Fluorescence Detector cysh. asp. thr. ser 5. glu 6. gly 7. ala 8. val 9. met 0. ile. leu. nle. tyr. phe 5. his 6. lys 7. trp 8. nh 9. arg Lithium Amino Acid Column 0 ml/hr with LiA, LiB, LiC at C, Fluorescence Detector Sodium Amino Acid Column (600/700 systems) 6 ml/hr with NaE, NaF, NaD at C, Fluorescence Detector. pser. tau. petn. asp 5. thr 6. ser 7. asn 8. glu 9. gln 0. aad. gly. ala. cit 8. hcy 9. ile 0. leu. tyr. phe. bala. baba 5. hcys 6. gaba. abu 5. val 6. cys 7. met 7. trp 8. etn 9. nh 0. hyl. amyl. orn. lys. -mhis 5. his 6. -mhis 7. ans 8. carn 9. arg cysh. meto. asp. meto 5. thr 6. ser 7. glu 8. gly 9. ala 0. val. met. ile. leu. nle 5. tyr 6. phe 7. glcnh 8. glanh 9. his 0. lys. trp. nh. arg Authorized Distributor

47 HPLC COLUMNSConcise Separations Ion Analysis Concise Separations ICSep Column for Ion Analysis Rugged polymeric substrate, stable in ph range of 0 to Solvent compatibility High efficiency Reproducibility Anion analysis with strong anion exchange columns Concise Separations Ion Chromatography (IC) columns have been designed to run on a variety of systems. They are tested to be compatible with Ion Chromatographs from: Metrohm, Dionex, Hach-Lachat, and Alltech. The selectivities have been optimized to be compatible with many of the common IC columns currently available. This includes columns that meet the requirements of E.P.A. methods 00 parts a and b, and E.P.A. method 00.. CONCISE SEPARATION COLUMNS FOR ANION ANALYSIS (STRONG ANION EXCHANGE COLUMNS) COLUMN COMPETITIVE COLUMNS APPLICATION ICSep AN00 Dionex ASA F-, Cl-, NO-, Br-, NO-, HPO-, SO-, By E.P.A. Method 00.0(a) ICSep AN Dionex AS9-HC F-, Cl-, NO-, Br-, NO-, HPO-, SO-, Low molecular weight, Organic acids in medium to high ionic strength matrices, Cr(III), Cr(VI) as CrO-, CrO- ICSep ANSC Dionex AS9-HC F-, Cl-, NO-, Br-, NO-, HPO-, SO-, Low molecular weight, Organic acids in medium to high ionic strength matrices ICSep AN Dionex AS Arsenate, Sulfite, Selenate, Arsenite, Selenite, F-, Cl-, NO-, Br-, NO-, HPO-, SO-, Low molecular weight Organic acids ICSep AN00B Dionex AS9 F-, Cl-, NO-, Br, NO-, HPO-, SO-, ClO-, ClO-, BrO- ICSep ION-0 ICSep AN-SDA F-, Cl-, NO-, Br-, NO-, HPO-, SO-, Low molecular weight, Organic acids in medium to high ionic strength matrices, Cr(III), Cr(VI) as CrO-, CrO- F-, Cl-, NO-, Br-, NO-, HPO-, SO-, Low molecular weight, Organic acids in medium to high ionic strength matrices, Cr(III), Cr(VI) as CrO-, CrO- AN-SS Alltech A- F-, Cl-, NO-, Br-, NO-, HPO-, SO-, By E.P.A. Method 00.0(a) RECOMMENDED ELUENTS COMMENTS Carbonate Good for drinking water method, EPA 00.0 a or 00.. Superb fluoride separation from water dip and other anions. Fast analysis times (under 9 min for 7 std anions) Carbonate Solvent compatible Sodium hydroxide eluents Solvent compatible Salicylic Acid Solvent compatible Solvent compatible Can be used with non-suppressed conductivity detection. Very good fluoride separation from water dip, great for toothpaste analysis. Great for trace anion work. Similar selectivity as the AN column but is solvent compatible for easy cleanup Superb fluoride, acetate & chloride resolution Good for drinking water method, EPA 00.0 b for oxyhalides. High capacity and unique selectivity Very good for non-suppressed detection Fast analysis times. No conductivity cell needed (UV) Created for phosphate & other anions in soft drinks, no need to dilute or pre-treat soft drink. Unlike other anion columns, colorings and additives will not foul column. Designed to replace Alltech A- anion column AN-HS F, Cl, NO, Br, PO, SO Carbonate Fast AN column with sulfate detection under four minutes AN-HC All inorganic anions including oxyhalides and low mw organic acids Solvent compatible AN00BHS For simple samples containing oxyhalides Solvent compatible ICSep AN00H F-, Cl-, NO-, Br-, NO-, HPO-, SO-, By E.P.A. Method 00.0(a) Carbonate Ideal for tough separations as extra capacity gives improved resolution capabilities. Superb fluoride, acetate & chloride resolution Fast AN00B column with sulfate under six minutes. Can resolve oxyhalides. Good for fast analysis times & high throughput Very high capacity AN00 column. Ideal for tough anion separations as extra capacity gives improved resolution capabilities. Superb anion resolution even with quick retention times. Good for drinking water method, EPA 00.0 a or 00.. Designed for trace anion analysis along with large amounts of Cl or NO. AN-HS.0 ml/min with.7 mm Na₂CO₃/.8 mm NaHCO at ambient, suppressed conductivity. chl. nitrate. pho. sul AN-HC.0 ml/min with.0 mm Na₂CO₃/.5 mm NaHCO at ambient, suppressed conductivity. flu. ace. chl. bromate 5. nitrite 6. bromide 7. nitrate 8. chl 9. pho 0. sul AN00BHS.0 ml/min with.0 mm Na₂CO₃ at ambient, suppressed conductivity. chl. nitrate. pho. sul 5 6 IC Sep AN00H.0 ml/min with.8 mm Na₂CO₃/. mm NaHCO at ambient, suppressed conductivity. flu. chl. nitrite. bro 5. nitrate 6. pho 7. sul Chrom Tech your chromatography specialists

48 Concise Separations Ion Analysis HPLC COLUMNS DESCRIPTION SIZE AN AN AN-SC AN00 AN00B ION-0 PEEK Column 5.5 x 50 mm ANX PEEK Column.6 x 50 mm ANX ANX ANX ANX PEEK Column.6 x 50 mm ANX PEEK Column (Fast).6 x 00 mm ANX SS Column.6 x 0 mm ANX Guard Cartridges (/pk) ANX ANX ANX ANX ANX ANX Guard Cartridge Holder ANX ANX ANX ANX ANX AXC Guard Column ANX ANX ANX-99-5 ANX ANX Replacement Frits (5/pk) AXC AXC AXC AXC AXC Guard Disc (5/pk) GRD GRD GRD GRD GRD Cartridge Kit (Column, Guard Cartridges (/pk), Column Coupler & Guard Cartridge Holder) ANX K ANX-99-85K ANX-99-85K ANX ANX K Column Kit (Column, Guard Column & Column Coupler) ANX K ANX-99-85K ANX-99-85K ANX ANX K Guard Kit (Universal Holder, Guard Cartridges (/pk) ANX DESCRIPTION SIZE AN-SDA AN-SS AN-HS AN-HC AN00BHS AN00H PEEK Column.6 x 00 mm ANX PEEK Column.6 x 50 mm ANX PEEK Column.6 x 50 mm ANX ANX ANX SS Column.6 x 00 mm ANX S Guard Cartridges (/pk) ANX ANX Guard Cartridge Holder ANX ANX Guard Column ANX ANX Replacement Frits (5/pk) AXC AXC AXC AXC Guard Disc (5/pk) GRD GRD GRD GRD Cartridge Kit (Column, Guard Cartridges (/pk), Column Coupler & Guard Cartridge Holder) ANX K Column Kit (Column, Guard Column & Column Coupler) ANX K ICSep AN. ml/min with.5 mm Na₂CO₃/.0 mm NaHCO at ambient, suppressed conductivity. flu. ace. chl. nitrite 5. bro 6. nitrate 7. pho 8. sul ICSep AN.0 ml/min with.7 mm Na₂CO₃/.8 mm NaHCO at ambient, suppressed conductivity. flu. chl. nitrite. bro 5. nitrate 6. pho 7. sul ICSep AN-SC.0 ml/min with.8 mm Na₂CO₃/.7 mm NaHCO at ambient, suppressed conductivity. flu. chl. nitrite. bro 5. nitrate 6. pho 7. sul ICSep AN00.0 ml/min with.8 mm Na₂CO₃/.7 mm NaHCO at ambient, suppressed conductivity. flu. chl. nitrite. bro 5. nitrate 6. pho 7. sul ICSep AN00B.0 ml/min with.0 mm Na₂CO₃ at ambient, suppressed conductivity flu. chlorite. bromate. chloride 5. nitrite 6. chlorate 7. nitrate 8. bromide 9. pho 0. sul ICSep ION-0. ml/min with.0 mm Salicylic Acid/5.0 mm Tris at ambient, UV 5. flu. car. chl. nitrite 5. bro 6. nitrate 7. pho 8. sul ICSep AN-SDA.0 ml/min with.0 mm Na₂CO₃/.5 mm NaHCO at ambient, suppressed conductivity. chl. nitrate. pho. sul AN-SS.0 ml/min with.0 mm Na₂CO₃/.0 mm NaHCO at ambient, suppressed conductivity. flu. ace. chl. nitrite 5. bro nitrate 7. pho 8. sul Authorized Distributor

49 HPLC COLUMNS Hamilton Reversed Phase Hamilton Reversed-Phase HPLC Columns Specification Chart HAMILTON REVERSED PHASE COLUMNS PACKING NAME SUPPORT MATERIAL PORE SIZE TEMP. LIMITS MOBILE PHASE LIMITS HxSil C8 Silica, end capped 0 Å 5-60 C ph % aqueous, organic modifier HxSil C8 Silica, end capped 0 Å 5-60 C ph % aqueous, organic modifier PRP-C8 C8 bonded to PSDVB* 00 Å 5-85 C ph. 0-00% aqueous, organic modifier PRP- PSDVB* 00 Å 5-85 C ph % aqueous, organic modifier PRP- PSDVB* 00 Å 5-85 C ph % aqueous, organic modifier PRP-h5 Fluorinated PSDVB* 00 Å 5-85 C ph. 0-00% aqueous, organic modifier *PSDVB is Poly(styrene-divinylbenzene) BUFFER STRENGTH N/A N/A MAXIMUM PRESSURE 6,000 PSI 6,000 PSI N 5,000 PSI N 5,000 PSI N 5,000 PSI N 5,000 PSI Hamilton HxSil C8 & HxSil C8 Silica Packing Hamilton HxSil C8 columns are well end-capped for retention time reproducibility and durability. HxSil C8 columns exhibit greater retention than most columns and enhance the separation of compounds that are not sufficiently retained on other C8 columns. Hamilton HxSil C8 columns are well end-capped for retention time reproducibility and durability. The C8 demonstrates excellent selectivity needed for complex sample mixtures and gives shorter retention times than a C8. SIZE (mm) Call Chrom Tech to custom build your Hamilton HPLC column HxSil C8 HxSil C8 μm 5 μm μm 5 μm.6 x 50 SS x 50 SS x 00 SS 790. x 00 SS x 50 SS x SS 796 Analytical Guard Column Analytical Guard Cartridge Holder SS Analytical Repl Cartridges SS (5/pk) Analytical Starter Kit SS Prep/Semiprep Guard Column Prep/Semiprep Guard Cartridge Holder SS Prep/Semiprep Repl Cartridges SS (/pk) Prep/Semiprep Starter Kit SS DESCRIPTION μm 5 μm 0 μm μm 5 μm 0 μm Bulk Resin ( gram) ) Analytical Starter Kit includes holder, cartridges ) Prep/Semiprep Starter Kit includes holder, cartridge 06 Chrom Tech your chromatography specialists

50 Hamilton Reversed Phase HPLC COLUMNS Hamilton PRP-C8 Columns Polymer Packing Superior resolution for purer oligos Extended column lifetime for increased productivity High loading capacity for time and cost savings High efficiency separations at any ph PRP-C8 SIZE (mm) 5 μm 0 μm. x 50 SS x 50 SS x 50 PEEK x 50 SS x 50 PEEK x 50 SS x 50 PEEK x 50 SS x 50 PEEK x 50 SS x 50 PEEK x 50 SS x 50 PEEK Analytical Guard Column 799 Analytical Guard Cartridge Holder SS 908 Analytical Guard Cartridge Holder PEEK 7977 Analytical Repl Cartridges SS (5/pk) Analytical Repl Cartridges PEEK (5/pk) Analytical Starter Kit SS Analytical Starter Kit PEEK Prep/Semiprep Guard Column 7998 Prep/Semiprep Guard Cartridge Holder SS Prep/Semiprep Repl Cartridges SS (/pk) Prep/Semiprep Starter Kit SS DESCRIPTION 5 μm 0 μm 0 μm Bulk Resin ( gram) ) Analytical Starter Kit includes holder, cartridges ) Semiprep/Prep Starter Kit includes holder, cartridge SPECIFICATIONS ANALYTE EXAMPLES Peptides, DNA, RNA, Oligonucliotides, Nucleotides, Vitamins, Steroids, Herbicides, Pharmaceutical compounds APPLICATIONS Organic compounds: small molecules (<,000 mw), pharmaceuticals, steroids, halides, vitamins, amino acid analysis, herbicides Rapid Separation of Basic Drug Compounds on PRP-C8 Column: Instrument: Mobile Phase: Flow Rate: Gradient: Temperature: Injection Vol: Detection: PRP-C8. x 50 mm, 5 µm Agilent 00 quaternary pump with UV detector A: 0 mm Diethylamine B: Mobile phase A + 95% ACN, 5% HO ml/min 0 to 00% B in 5 minutes Ambient 0 μl UV at 65 nm 5 6. Nicotine. Metoprolol. Quinine. Doxylamin 5. Dexmethorphan 6. Amitriptyline Authorized Distributor

51 HPLC COLUMNS Hamilton Reversed Phase Hamilton PRP- Columns Polymer Packing Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C8 HPLC columns. Since Hamilton polymers are made entirely of poly styrene-divinylbenzene, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50 80% of an oligonucleotide is recovered on a C8 column, the equivalent PRP column recovers 95% or greater of the same sample. Unlike silica-based C8 or C8 columns, PRP- has no stationary phase coating. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C8 columns. PRP- SIZE (mm) 5 μm 7 μm 0 μm 0 μm 0.6 x 50 SS x 50 SS x 50 SS 799. x 50 SS x 75 SS x 50 SS x 00 SS x 50 SS x 05 SS x 00 SS x 50 SS x 50 SS x 00 SS x 50 SS x 50 SS x 00 SS x 50 SS 79. x 50 SS x 00 SS x 50 SS x 50 SS Analytical Guard Column 7986 Analytical Guard Cartridge Holder SS 908 Analytical Guard Cartridge Holder PEEK 7977 Analytical Repl Cartridges SS (5/pk) 795 Analytical Repl Cartridges PEEK (5/pk) 798 Analytical Starter Kit SS 797 Analytical Starter Kit PEEK 797 Prep/Semiprep Guard Column 799 Prep/Semiprep Guard Cartridge Holder SS Prep/Semiprep Repl Cartridges SS (/pk) 79 Prep/Semiprep Starter Kit SS 79 DESCRIPTION 5 μm 7 μm 0 μm 0 μm 0 0 μm 0 50 μm μm Bulk Resin ( gram) ) Analytical Starter Kit includes holder, cartridges ) Prep/Semiprep Starter Kit includes holder, cartridge Call Chrom Tech to custom build your Hamilton HPLC column SPECIFICATIONS ANALYTE EXAMPLES Polycyclic aromatic hydrocarbons (PAH), Ionizable organic compounds, steroids, peptide fragments APPLICATIONS Organic compounds: small molecule (<,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides 08 Chrom Tech your chromatography specialists

52 Hamilton Reversed Phase HPLC COLUMNS Hamilton PRP- & PRP-h5 Columns Polymer Packing Hamilton s PRP- is a polymeric reversed-phase HPLC column designed for the purification and isolation of proteins and peptides with very good recovery (> 90%). It is based off of the PRP- but utilizes a 00 Å pore size rather than the PRP- s 00 Å pore size. The highly inert polymeric packing poly(styrene-divinylbenzene) enhances protein recovery because there are no silanol groups on the support to cause irreversible protein adsorption. The PRP-h5 utilizes the PRP- as its base with a pentafluorinated modification, making it more hydrophobic in nature and delivers a selectivity difference from standard silica C8 stationary phases. This difference is especially pronounced for halogenated solutes. PRP- PRP-h5 SIZE (mm) PRP- PRP-h5 0 μm 0 μm 5 μm. x 50 SS x 00 SS x 50 SS x 50 SS x 00 SS x 05 SS x 50 SS x 50 SS x 00 SS x 50 SS 796. x 50 SS x 50 SS x 50 SS x 00 SS 7970 Analytical Guard Column Analytical Guard Cartridge Holder SS Analytical Guard Cartridge Holder PEEK 7977 Analytical Repl Cartridges SS (5/pk) Analytical Repl Cartridges PEEK (5/pk) 7995 Analytical Starter Kit SS Analytical Starter Kit PEEK 799 Prep/Semiprep Guard Column Prep/Semiprep Guard Cartridge Holder SS Prep/Semiprep Repl Cartridges SS (/pk) Prep/Semiprep Starter Kit SS DESCRIPTION 0 μm 0 μm 5 μm 0 μm Bulk Resin ( gram) ) Analytical Starter Kit includes holder, cartridges ) Prep/Semiprep Starter Kit includes holder, cartridge Five Proteins at 80 C on PRP-h5 Column: PRP-h5.6 x 50 mm, 5 µm 796 Mobile Phase: A: DI Water, 0.% TFA B: Acetonitrile, 0.% TFA Flow Rate: ml/min Gradient: to 55% B in 0 min. Temperature: 80 C Injection Vol: 5 μl Detection: UV at 0 nm. Ribonuclease A. Cytochrome C. apo-transferrin. Myoglobin 5 5. Carbonic Anhydrase SPECIFICATIONS ANALYTE EXAMPLES PRP-: Globular proteins, Albumins, Antibody fragments, Tryptic digests, DNA, RNA oligomers, Synthetic high mw polymers PRP-h5: Oligonucleotides, Angiotensin, apo-transferrin, Apomyoglobin (equine), Carbonic anhydrase, Cytochrome C, Myoglobin, Ribonuclease A APPLICATIONS PRP-: Organic compounds: large molecules (>,000 mw), peptides, proteins, protein digests, protected and de-protected oligonucleotides, nucleic acids PRP-h5: Organic compounds: macromolecules (>,000 mw), pharmaceuticals, protein digests, tryptic digests, proteomics Call Chrom Tech to custom build your Hamilton HPLC column Five Proteins on PRP- Column: PRP-. x 50 mm, 5 µm 7966 Mobile A: 0.0% TFA in water ph.0 Phase: B: 0.% TFA in acetonitrile Flow Rate: ml/min Gradient: 5 to 50% B in 5 min. Hold min. Temp: Ambient Inj Vol: 00 μl Detection: UV at 5 nm. Ribonuclease A. Cytochrome C. Lysozyme. Myoglobin 5. Ovalbumin 5 Authorized Distributor

53 HPLC COLUMNS Hamilton Ion Exchange Hamilton Ion Exchange HPLC Columns Specification Chart HAMILTON ANION EXCHANGE COLUMNS PACKING NAME SUPPORT MATERIAL ANION EXCHANGE COLUMNS PRP-X00 PRP-X0 PRP-X500 PRP-X600 RCX-0 RCX-0 PSDVB* with Tri-methyl ammonium Exchanger PSDVB* with Tri-methyl ammonium Exchanger Poly(meth-acryl amido-propyl Trimethyl-ammonium chloride) Poly (di-methyl amido-propyl meth-acrylamide) PSDVB* with Tri-methyl ammonium Exchanger PSDVB* with Tri-methyl ammonium Exchanger CATION EXCHANGE COLUMNS EXCHANGE CAPACITY PORE SIZE TEMP. LIMITS MOBILE PHASE LIMITS 0.9meq/gm 00 Å ph Dependent** ph % aqueous, organic modifier 0.meq/gm 00 Å ph Dependent** ph % aqueous, organic modifier.6meq/gm Superficially porous ph Dependent** ph % aqueous, organic modifier.6meq/gm Superficially porous ph Dependent** ph % aqueous, organic modifier 0.5meq/gm 00 Å ph Dependent** ph % aqueous, organic modifier.0meq/gm 00 Å ph Dependent** ph % aqueous, organic modifier PRP-X00 PSDVB* Sulfonate Exchanger 5ueq/gm 00 Å 5-60 C ph % aqueous, organic modifier PRP-X00 PSDVB* Sulfonate Exchanger.5meq/gm N/A 5-60 C ph % aqueous, organic modifier PRP-X800 PSDVB* Itaconate Exchanger.6meq/gm 00 Å 5-60 C ph % aqueous, organic modifier HC-0 Calcium HC-75 Calcium HC-75 Hydrogen BUFFER STRENGTH N N N N N N N N N MAXIMUM PRESSURE 5,000 PSI 5,000 PSI 5,000 PSI 5,000 PSI 5,000 PSI 5,000 PSI 5,000 PSI 5,000 PSI 5,000 PSI PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 5-90 C 00% Water Water,000 PSI PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 5-90 C 00% Water 0-0% Acetonitrile PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 5-90 C 00% Water 0-0% Acetonitrile HC-75 Lead PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 5-90 C 00% Water 0-0% Acetonitrile *PSDVB is Poly(styrene-divinylbenzene) **ph -7.9 Temp C; ph 8- Temp. 5-0 C Water Water Water 00 PSI 00 PSI 00 PSI Arsenic Speciation on Hamilton PRP-X00 Organic arsenic compounds added to feed stocks of chicken and poultry pose serious environmental and ecological threats. National and worldwide health organizations, such as the United States Environmental Protective Agency and the US Food and Drug Administration, have recently implemented more stringent concentration limits for arsenic species in drinking water and foodstuffs. Analysis of arsenic species is made challenging due to diverse sample matrices. To circumvent these problems, an HPLC-ICPMS method has been developed. Resolution of arsenic compounds is achieved by ion exchange chromatography on a Hamilton PRP-X00 column, a 55% cross-linked polystyrene-divinylbenzene copolymer functionalized with quaternary ammonium anion-exchanger group. Detection is accomplished by inductively coupled plasma-mass spectrometry. Intensity (cps) 9,8. 08, ,7.00 DMA V + DMA II MMA II + MMA V AS V Column: Mobile Phase: Flow Rate: Injection Vol: Detection: PRP-X00.6 x 50 mm, 5 µm 798 mm (NH)CO for 0 min 0 mm (NH)CO for min mm (NH)CO for 7 min.0 ml/min 50 μl, 00 μg/l of each standard ICP-MS -, , AS III Time (min) MMTA Borrowed (with permission) from P. Alava et al. Biomed. Chromatogr. 0; 6: Chrom Tech your chromatography specialists

54 Hamilton Ion Exchange HPLC COLUMNS Hamilton PRP-X00 and PRP-X0 Columns Anion Hamilton PRP-X00 and PRP-X0 are highly stable, inert materials. The PRP-X00 can be used with virtually any HPLC or ion chromatograph, including dedicated IC units. Technological advancements in modern polymer chemistry now deliver a more rugged column with exceptionally higher separation efficiencies than earlier predecessors. PRP-X00 and PRP-X0 columns are well suited for use in systems employing suppressed/non-suppressed conductivity, electrochemical, UV, and ICP-MS detection. Chromatographers currently using wet chemical or colorimetric methods will find ion chromatography greatly reduces sample pretreatment and improves the accuracy and precision of results. Fast Separation of Common Anions on PRP-X00 SIZE (mm) 5. Fluoride. Chloride. Nitrite. Bromide 5. Nitrate PRP-X00 PRP-X0 PRP-X0S 5 μm 0 μm 0 μm 7 μm 7 μm. x 50, 00 Å x 50 PEEK x 50 PEEK x 50 SS x 50 SS x 00 SS x 50 SS x 50 SS x 50 SS 79. x 50 PEEK x 00 PEEK 797 Analytical Guard Column Analytical Guard Cartridge Holder SS Analytical Guard Cartridge Holder PEEK Analytical Repl Cartridges SS (5/pk) Analytical Repl Cartridges PEEK (5/pk) Analytical Starter Kit SS Analytical Starter Kit PEEK Prep/Semiprep Guard Column Prep/Semiprep Guard Cartridge Holder SS Prep/Semiprep Repl Cartridges SS (/pk) Prep/Semiprep Starter Kit SS DESCRIPTION 5 μm 0 μm 0 μm 7 μm 7 μm Bulk Resin ( gram) Eluent Concentrate PRP-X00 Anion Exchange () 60 ml bottle (6) 60 ml bottles ) Analytical Starter Kit includes holder, cartridges ) Prep/Semiprep Starter Kit includes holder, cartridge Call Chrom Tech to custom build your Hamilton HPLC column Column: Mobile Phase: PRP-X00. x 50 mm, 5 µm 7950 mm parahydroxybenzoic acid, ph 8.5 /% acetonitrile Flow Rate: Gradient: 0.8 ml/min Isocratic Temperature: 80 C Injection Vol: μl Sample Concentration: 0. mg/ml Detection: UV at 05 nm SPECIFICATIONS ANALYTE EXAMPLES Halides, Polarizable anions, Organic acids, Organic and inorganic arsenic species INDUSTRIES Pharmaceutical, Environmental, Medical Research, Food and Beverage APPLICATIONS PRP-X00/X0 Organic and inorganic anions, organic acids, organic and inorganic arsenic species TECH TIP The difference between PRP-X0 versus PRP-X0S columns PRP-X0 columns are equilibrated with a mm p-hydroxybenzoic acid ph 8.5 mobile phase and are ready for use with conductivity or indirect UV detection methods. PRP-X0S columns are equilibrated with a. 7 mm sodium bicarbonate,.8 mm sodium carbonate, 0. mm sodium thiocyanate mobile phase and are ready for use with suppressed conductivity detection methods. Authorized Distributor

55 HPLC COLUMNS Hamilton Ion Exchange Hamilton PRP-X500 and PRP-X600 Columns Anion PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses typically seen on other commercially available protein HPLC columns. PRP-X600 is a superficially porous weak-base anion exchange support that separates DNA oligomers according to negative charge. Because the PRP-X600 is a weak anion exchange (WAX) resin, the exchange capacity of the resin is ph dependent. Lowering the ph will reduce the binding of proteins, reducing the run time for a complete separation. Change the mobile phase composition to alter the retention of DNA oligomers. Rapid gradient changes typically lower column efficiency; however, biomolecules run in this fashion on the PRP-X500 show very favorable separation efficiency with much shorter run times. SIZE (mm) PRP-X500 7 μm PRP-X600 7 μm.6 x 50 PEEK x 50 PEEK x 50 PEEK 790 Analytical Guard Cartridge Holder PEEK Analytical Repl Cartridges PEEK (5/pk) Analytical Starter Kit PEEK DESCRIPTION 7 μm 0 μm 0 50 μm 7 μm 0 μm 0 50 μm Bulk Resin ( gram) ) Analytical Starter Kit includes holder, cartridges Call Chrom Tech to custom build your Hamilton HPLC column PRP-X500 SPECIFICATIONS ANALYTE EXAMPLES PRP-X600 PRP-X500: Myoglobin, Bovine serum albumin, Conalbumin, Ovalbumin PRP-X600: Synthetic RNA, DNA oligonucleotides, Proteins and peptides, Ovalbumin, DNA fragments, Oligonucleotides APPLICATIONS PRP-X500: Proteins/Peptides Single Stranded/Double Stranded RNA/DNA PRP-X600: Nucleic acids such as single stranded/double stranded RNA and DNA Peptides and proteins Myoglobin, Conalbumin and Dog Albumin on PRP-X500 Oligodeoxycytidylate (dc) -8 on PRP-X600. dc 5 7. dc 6. dc. dc5 5. dc6 6. dc7 7. dc8 Column: PRP-X600.6 x 50 mm, 7 µm 7960 Mobile Phase: A: 85/5 00 mm TRIS, ph 8.0/acetonitrile B: 85/5 00 mm TRIS, ph 8.0,.5 M lithium chloride/acetonitrile Flow Rate: ml/min Gradient: 0 to 0% B in 0 minutes Temperature: Ambient Injection Vol: 0 μl Sample Concentration: 00 μg/ml Detection: UV at 60 nm Column: PRP-X500.6 x 50 mm, 5 µm 797 Mobile A: 0 mm Tris Phase: ph 9.0 B: 70 mm Tris ph 9.0, 0.5 N Sodium Chloride Flow Rate: ml/min Gradient: 0 to 50% B in.5 min. Hold for.5 min. Temp: Ambient Inj Vol: 0 μl Detection: UV at 5 nm. Myoglobin 7 μg. Conalbumin 7 μg. Dog albumin 77 μg Chrom Tech your chromatography specialists

56 Hamilton Ion Exchange HPLC COLUMNS Hamilton RCX-0 and RCX-0 Columns Anion The Hamilton RCX-0 and RCX-0 carbohydrate analysis columns are designed for the isocratic or gradient separation of carbohydrates. The exchange capacity of the RCX-0/ RCX-0 is greater than that of the PRP-X00, leading to characteristics better suited for the separation of carbohydrates. Simple samples with two or three carbohydrates can be quickly separated isocratically, while more complex samples require gradient elution to fully resolve all the analytes of interest. When an isocratic method is used with a conductivity, refractive index, ultraviolet, or pulsed amperometric detector (PAD), mono and disaccharides such as glucose, fructose, sucrose and lactose can be quickly determined. RCX-0/0 SIZE (mm) RCX-0 7 μm RCX-0 7 μm.6 x 50 PEEK x 50 PEEK x 50 SS Analytical Guard Column 799 Analytical Guard Cartridge Holder SS 908 Analytical Guard Cartridge Holder PEEK Analytical Repl Cartridges SS (5/pk) 796 Analytical Repl Cartridges PEEK (5/pk) Analytical Starter Kit SS 796 Analytical Starter Kit PEEK DESCRIPTION 7 μm 0 μm 7 μm 0 μm Bulk Resin ( gram) ) Analytical Starter Kit includes holder, cartridges SPECIFICATIONS ANALYTE EXAMPLES Arabinose, Galactose, Lactose, Maltose, Sucrose APPLICATIONS Carbohydrates, polysaccharides, sugar oligomers up to DP8 mono and disaccharides Call Chrom Tech to custom build your Hamilton HPLC column Jerusalem Artichoke Tubers on RCX-0 Corn Syrup Sugars on RCX-0. DP. DP5. DP0. DP5 Column: Mobile Phase: Flow Rate: RCX-0. x 50 mm, 5 µm 790 A: 60 mm Sodium Hydroxide B: 60 mm Sodium Hydroxide with 500 mm Sodium Acetate ml/min Gradient: 0 to 00% B in 0 minutes Temperature: Injection Vol: Detection: Ambient 0 μl Pulsed amperometric, dual gold electrode: E = 50 mv T = msec E = 800 mv T = 66 msec E = -600 mv T = 99 msec 5 6. Glucose. Fructose. Maltose. Maltotriose 5. Maltotetraose 6. Maltopentaose 7. Maltohexaose 8. Maltoheptaose 9. Maltooctaose 0. Maltononaose 7. Maltodecaose Column: Mobile Phase: Flow Rate: Gradient: Temperature: Injection Vol: Detection: RCX-0.6 x 50 mm, 5 µm 7970 A: 60 mm Sodium Hydroxide B: 500 mm Sodium Acetate in A ml/min 00% A for min, then 0 to 00% B ( 5 min.) Ambient 50 μl Pulse damperometric, dual gold electrode E = 50 mv T = 66 msec E = 900 mv T = 66 msec E = -850 mv T = 500 msec Authorized Distributor

57 HPLC COLUMNS Hamilton Ion Exchange Hamilton PRP-X00 & PRP-X00 Columns Cation Hamilton PRP-X00 cation exchange HPLC columns are designed for rapid, high resolution separation of alkali and alkaline earth metals. The alkali metals and ammonium are completely resolved in less than five minutes, and the alkaline earth cations separate in under four minutes. Since the mobile phase conditions are different and unique for each of the groups of cations, interferences between these groups are eliminated. The PRP-X00 column provides a fast separation for glyphosate and its metabolites. The exchange capacity of the PRP-X00 is greater than that of the PRP-X00, leading to characteristics better suited for the separation of glyphosate. It also performs well in other separations, such as inositol and sugar alcohols. The column does not have to be heated to 65 C and operates well at room temperature, so a column heater is not necessary for this method. PRP-X00 columns do not require the use of methanol in the mobile phase, and they cost much less than other glyphosate columns. SIZE (mm) PRP-X00 7 μm PRP-X00 0 μm.6 x 50 PEEK x 50 PEEK 798. x 50 SS x 50 SS x 00 SS 796. x 50 SS x 50 SS Analytical Starter Kit SS Analytical Repl Cartridges SS (5/pk) Analytical Starter Kit PEEK Analytical Repl Cartridges PEEK (5/pk) Analytical Guard Column Analytical Guard Cartridge Holder PEEK Analytical Guard Cartridge Holder SS Prep/Semiprep Guard Column Prep/Semiprep Guard Cartridge Holder SS Prep/Semiprep Repl Cartridges SS (/pk) Prep/Semiprep Starter Kit SS DESCRIPTION 7 μm 0 μm 0 50 μm 0 μm 0 μm Bulk Resin ( gram) ) Analytical Starter Kit includes holder, cartridges ) Prep/Semiprep Starter Kit includes holder, cartridge Call Chrom Tech to custom build your Hamilton HPLC column PRP-X00/X00 SPECIFICATIONS ANALYTE EXAMPLES PRP-X00: Calcium, Cesium, Potassium, Sodium PRP-X00: Glyphosate, Maltose, Xylitol, Mannitol APPLICATIONS PRP-X00: Inorganic and organic cations using conductivity or UV detection, alkali and alkaline earth metals. Separate mono or divalent cations depending on mobile phase conditions from 0 ppb to 00 ppm. PRP-X00: Glyphosate and its metabolite in drinking water. The PRP-X00 provides unique hydrophilic interaction separations. Cations, inorganic and organic using conductivity or UV detection. Glyphosate on PRP-X00 Column: PRP-X00. x 50 mm, 5 µm 797 Mobile Phase: M Monobasic potassium phosphate Flow Rate: 0.5 ml/min Gradient: Isocratic Temperature: Ambient Injection Vol: 00 μl Detection: Excitation λ 8 nm, Emission λ 55 nm Monovalent Cations on PRP-X00 Column: Mobile Phase: Flow Rate: Gradient: Temperature: Injection Vol: Detection: PRP-X00. x 50 mm, 5 µm 79 (.:) mm Nitric acid:methanol ml/min Isocratic Ambient 00 μl Conductivity 5. Lithium. Sodium. Ammonium. Potassium 5. Cesium. Glyphosate. Aminomethylphosphonic acid Chrom Tech your chromatography specialists

58 Hamilton Ion Exchange HPLC COLUMNS Hamilton PRP-X800 Columns Cation The PRP-X800 is a polymeric cation exchange column functionalized with itaconic acid that performs the isocratic separation of mono and divalent cations such as lithium, sodium, ammonium, potassium, magnesium and calcium. The column offers excellent durability, is stable to any concentration organic solvent, and enables dynamic control of exchange capacity. Detection is via conductivity or indirect UV, depending on the mobile phase. SIZE (mm). x 50 SS x 50 SS Analytical Guard Column 799 Analytical Guard Cartridge Holder SS 908 Analytical Guard Cartridge Holder PEEK 7977 Analytical Repl Cartridges SS (5/pk) 798 Analytical Repl Cartridges PEEK (5/pk) 798 Analytical Starter Kit SS 7980 Analytical Starter Kit PEEK 798 ) Analytical Starter Kit includes holder, cartridges Call Chrom Tech to custom build your Hamilton HPLC column 7 μm PRP-X800 SPECIFICATIONS ANALYTE EXAMPLES Mono and divalent metal cations (e.g., sodium, potassium, calcium) Transitions metals (e.g., iron, manganese, nickel, copper, zinc) APPLICATIONS Mono and divalent transition metals in the same run. Transition metals (e.g., manganese, zinc, cobalt and cadmium) are also resolved on the column using an ethylenediamine/tartaric acid mobile phase and conductivity detection. Mono and Divalent Cations on PRP-X800 TECH TIP. Lithium. Sodium. Ammonium. Potassium 5. Magnesium 6. Calcium Column: Mobile Phase: Flow Rate: Gradient: Temperature: PRP-X800. x 50 mm, 5 µm 7988 mm Cupric Sulfate 0.8 ml/min Isocratic Ambient The PRP-X800 is available in PEEK hardware as a custom order. Contact Chrom Tech for more information. Injection Vol: 0 μl Sample Concentration: All compounds are 5 ppm Detection: Indirect UV at 0 nm 5 6 Authorized Distributor

59 HPLC COLUMNS Hamilton Ion Exchange Hamilton HC-0 Ca + and HC-75 (H +, Ca +, Pb + ) Columns Cation HC-0 columns separate compounds through size exclusion. The % cross-linked HC- 0 uses size exclusion as the primary mechanism of separation. The larger carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later. HC-75 columns separate compounds through ligand exchange. The 8% cross-linked HC-75 uses ligand exchange as the primary mechanism of separation. The different forms of the HC-75 (Hydrogen, Calcium and Lead) each provide a unique selectivity for separating varying types of charged analytes based on electronegativity toward the counterion. The larger carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later. Pb +, H+ or Ca + PRP-X00/X00 Size (mm) HC-0 (Ca + ) HC-75 (Ca + ) HC-75 (H + ) HC-75 (Pb + ) 9 μm 0 5 μm 9 μm 9 μm 9 μm 7.8 x 05 SS x 00 SS x 50 SS Prep/Semiprep Guard Column Prep/Semiprep Guard Cartridge Holder SS Prep/Semiprep Repl Cartridges SS (/pk) Prep/Semiprep Starter Kit SS SPECIFICATIONS ANALYTE EXAMPLES Ethanol, Maltohexose citric acid, Glucose fructose, Aribinose, Sorbitol, Acetic acid APPLICATIONS Carbohydrates, sugar oligomers up to DP8. Mono and disaccharides, organic acids, sugars, and sugar alcohols DESCRIPTION 9 μm 0 5 μm 9 μm 9 μm 9 μm Bulk Resin ( gram) ) Prep/Semiprep Starter Kit includes holder, cartridge Call Chrom Tech to custom build your Hamilton HPLC column Chewing Gum Sugars on HC-75 Ca+ Organic Acids by USP L7 on HC-75 H+ 5. Sucrose. Glucose. Fructose. Mannitol. Citric acid. Lactic acid. Acetic acid 5. Sorbitol Column: HC-75 Calcium Form 7.8 x 05 mm, 5 µm 796 Mobile Phase: Deionized water Flow Rate:. ml/min Gradient: Isocratic Temperature: 90 C Injection Vol: μl Detection: Refractive index Column: HC-75 Hydrogen Form. x 50 mm, 5 µm 7976 Mobile Phase: 0.0 N sulfuric Flow Rate: 0.5 ml/min Gradient: Isocratic Temperature: 60 C Injection Vol: 0 μl Sample Concentration: All compounds are 50 ppm Detection: UV at 0 nm 6 Chrom Tech your chromatography specialists

60 Hamilton Ion Exclusion HPLC COLUMNS Hamilton Ion Exclusion HPLC Columns Specification Chart HAMILTON ION EXCLUSION COLUMNS PACKING NAME SUPPORT MATERIAL EXCHANGE CAPACITY PORE SIZE TEMP. LIMITS MOBILE PHASE LIMITS PRP-X00 PSDVB* Sulfonate Exchanger 0.7meq/gm 00 Å 5-60 C ph % aqueous, organic modifier *PSDVB is Poly(styrene-divinylbenzene) BUFFER STRENGTH N MAXIMUM PRESSURE 5,000 PSI Hamilton PRP-X00 Ion Exclusion Hamilton PRP-X00 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrene-divinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X00 is accomplished by three modes: Hydrogen Bonding The attraction and retention of sample compounds by the negatively charged sulfonate group. Reversed-Phase The interaction and retention of the sample compounds by the non-polar polymeric support. Ion Exclusion The process in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and nonionic compounds elute later. SIZE (mm) 7 μm.6 x 50 PEEK x 50 SS 796. x 50 SS 7965 Analytical Guard Column 7989 Analytical Guard Cartridge Holder SS 908 Analytical Guard Cartridge Holder PEEK 7977 Analytical Repl Cartridges SS (5/pk) 795 Analytical Repl Cartridges PEEK (5/pk) 797 Analytical Starter Kit SS 7960 Analytical Starter Kit PEEK 797 Prep/Semiprep Guard Column 7995 Prep/Semiprep Guard Cartridge Holder SS Prep/Semiprep Repl Cartridges SS (/pk) 790 Prep/Semiprep Starter Kit SS 799 DESCRIPTION 7 μm 0 μm Bulk Resin ( gram) ) Analytical Starter Kit includes holder, cartridges ) Prep/Semiprep Starter Kit includes holder, cartridge SPECIFICATIONS ANALYTE EXAMPLES Acetic acid, Acrylamide, Citric acid, Oxalacetic acid, Ethanol, Propanol APPLICATIONS Organic Acids on PRP-X00 Column: Mobile Phase: Flow Rate: Gradient: Temperature: Injection Vol: Detection: PRP-X00 Organic acids and alcohols PRP-X00. x 00 mm, µm 7988 mn Sulfuric acid ml/min Isocratic Ambient 0 μl UV at 0 nm. Tartaric acid. Malic acid. Citric acid. Lactic acid Call Chrom Tech to custom build your Hamilton HPLC column 5. Acetic acid 5 Authorized Distributor

61 HPLC COLUMNSImtakt Presto FF-C8 Imtakt Presto FF-C8 High resolution µm non-porous ODS column Reversed-phase separation for bio- and synthetic-polymers up to 0 MDa Different selectivity from porous ODS columns High-efficiency with low flow rate compatible with conventional HPLC systems Imtakt has developed a novel µm, non-porous, high resolution ODS column. There are several shortcomings for porous ODS columns for polymer separation: Poor peak shape of solutes due to wide range in pore size distribution Poor recovery of solutes due to micro-pores and meso-pores Reduced column efficiency due to high mass transfer resistance Presto FF-C8 can overcome these shortcomings for polymer separation. This high resolution, non-porous ODS column is quite different from conventional ODS columns, and will create new opportunities for st century separation science. IMTAKT PRESTO FF-C8 µm NON-POROUS SILICA, OCTADECYL LIGAND, END-CAPPED LENGTH (mm) mm ID mm ID mm ID.6 mm ID 6 mm ID 0 mm ID 50 FF06 FF06 FF06 FF006 FF066 FF0P6 50 FF05 FF05 FF05 FF005 FF065 FF0P5 00 FF0 FF0 FF0 FF00 FF06 FF0P 75 FF0 FF0 FF0 FF00 FF06 FF0P 50 FF0 FF0 FF0 FF00 FF06 FF0P 0 FF0 FF0 FF0 FF00 FF06 FF0P 0 FF09 FF09 FF009 0 FF00 FF00 FF000 Micro/Nano columns are also available Monoclonal & Polyclonal Antibodies mau Monoclonal antibody (anti-derf) Monoclonal antibody (anti-human LH) Monoclonal antibody (anti-afp) Polyclonal antibody (human serum) Column: Imtakt Presto FF-C8 50 x mm Mobile A: water/ Phase: trifluoroacetic acid = 00/0. B: acetonitrile/ trifluoroacetic acid = 00/0. 0% B (0 0 min) Flow Rate: 0. ml/min (7MPa) Temp: 80 C Detection: 0 nm TECH TIP Recommendations for Presto FF-C8 Presto FF-C8 is a non-porous ODS column consisting of um non-porous silica particles. The specific surface area is much lower than conventional porous ODS columns. As a result, eluent composition should be optimized in order to obtain retention equivalent to a porous ODS column. Presto FF-C8 works great at low flow rates and can be used on conventional HPLC systems. Higher flow rates (i.e. same flow rate as porous ODS column) will require the use of an UHPLC system. Mobile Phase Composition Retention is greatly reduced on non-porous ODS due to its extremely low surface area. In order to get similar retention to porous ODS, organic solvent ratio should be reduced. Flow Rate Because Presto FF-C8 is nonporous (and thus no diffusion in pores), excellent performance is also achieved at lower flow rates and improves resolution and sensitivity. Retention of Polar Compounds Presto FF-C8 has a disadvantage for isocratic elution due to low surface area. It is therefore recommended to use gradient elution as often as possible. Recommendations for HPLC Operation It is recommended that low dispersion systems and high pressure binary pumps are used with Presto FF-C min 8 Chrom Tech your chromatography specialists

62 Imtakt Cadenza HPLC COLUMNS Imtakt Cadenza CD-C8 Unique ligand density provides excellent steric selectivity High efficiency µm column with low back-pressure Excellent for impurity testing or isomer separation Selectivity ideal for small molecule pharmaceutical testing or drugs of abuse Isomer Separation Column: Imtakt Cadenza CD-C8 CDOU.0 x 75 mm,.0 µm Mobile Phase: MeOH/water = 55/5 Steroid Separation. androstene-, 7 -dione. testosterone Flow Rate: Temp: 0 C 0. ml/min. α-hydroxyprogesterone Detection: UV, 70 nm Cadenza CD-C8 excels at separating structural isomers.. methylparaben. ethylparaben. isopropylparaben. propylparaben 5. isobutylparaben 6. butylparaben As shown in this separation example, when one compares the separation of paraben isomer structures such as propylparabens and butylparabens, Cadenza provides more plates and better resolution in half the column length. Cadenza CD-C8 offers quicker results and greater sensitivity under the same separation conditions used for a conventional column. Column: Mobile Phase: Flow Rate: Detection:. progesterone Imtakt Cadenza CD-C8 CD0U 75 x mm,.0 µm A: 0% methanol, B: methanol 0 50% B (0 0.5 min) 00% B (.5 min) 0. ml/min UV, 60 nm IMTAKT CADENZA CD-C8 - µm COLUMN ID GUARD HOLDER GUARD CARTRIDGE 0 mm 0 mm 0 mm 50 mm 75 mm 00 mm 50 mm 50 mm CADENZA CD-C8 µm PRESSURE LIMIT UP TO 5,000 PSI (,0 BAR).0 mm GCH0S GCCD0S CD0U CD0U CD0U CD0U CD05U CD06U CADENZA CD-C8 µm PRESSURE LIMIT UP TO 7,500 PSI (57 BAR).6 mm GCH0S GCCD0S CD000T CD009T CD00T CD00T CD00T CD00T CD005T CD006T.0 mm GCH0S GCCD0S CD00T CD09T CD0T CD0T CD0T CD0T CD05T CD06T.0 mm GCH0S GCCD0S CD00T CD09T CD0T CD0T CD0T CD0T CD05T CD06T.5 mm GCH0S GCCD0C CD07T CD07T CD07T CD07T CD075T CD076T.0 mm GCH0S GCCD0C CD0T CD0T CD0T CD0T CD05T CD06T CADENZA CD-C8 5 µm PRESSURE LIMIT UP TO,000 PSI (06 BAR) 8.0 mm GCH0M GC5CD0M 5CD0R6 0.0 mm GCH0M GC5CD0M 5CD0Q 5CD0Q 5CD0Q5 5CD0Q6 0.0 mm GCH0M GC5CD0M 5CD0P 5CD0P 5CD0P 5CD0P 5CD0P5 5CD0P6 6.0 mm GCH0S GC5CD0S 5CD06 5CD06 5CD06 5CD06 5CD065 5CD066.6 mm GCH0S GC5CD0S 5CD00 5CD00 5CD00 5CD00 5CD005 5CD006.0 mm GCH0S GC5CD0S 5CD05 5CD06.0 mm GCH0S GC5CD0S 5CD0 5CD0 5CD0 5CD0 5CD05 5CD06.0 mm GCH0S GC5CD0S 5CD0 5CD0 5CD0 5CD0 5CD05 5CD06.5 mm GCH0S GC5CD0C 5CD07 5CD07 5CD07 5CD07 5CD075 5CD076.0 mm GCH0S GC5CD0C 5CD0 5CD0 5CD0 5CD0 5CD05 5CD06 All of Imtakt stationary phases can also be made in the following internal diameters: 0.05 mm, mm, 0. mm, 0. mm, and 0.5 mm. Authorized Distributor

63 HPLC COLUMNSImtakt Intrada Intrada Amino Acids Separation Column The world s first specialty column for intact amino acid analysis via LC-MS LC-MS Analysis of amino acids No derivatization required Pure µm spherical silica phase designed for amino acid analysis Intrada Amino Acid high-throughput columns provide amazing speed, selectivity, and convenience. The next generation amino acid analysis method for clinical amino acid biomarkers, fermented materials, and botanical amino acids is here. Separation of Leucine (Da) isomers 55 Amino Acids (standard samples) Intrada Amino Acid 50 x mm % B (0- min) 00% B ( 5 min) 0. ml/min ( MPa) 5 C, 5 µl (0.N HCI) ESI (positive, m/z.0) A: MeOH/Water/Formic acid (85/5/0.) B: ACN/00mM Ammonium formate (0/80) Intrada Amino Acid 50 x mm 0% B (0.5 min) 0-7% B ( min) 00% B (6.5 0 min) 0.6 ml/min (6MPa) 5 C, 5 µl ( µmol/ml) ESI (SIM) A: ACN/THF/5mM Ammonium formate/ Formic acid (9/75/6/0.) B: ACN/00mM Ammonium formate (0/80) Separation of GABA (0Da) isomers Intrada Amino Acid 00 x mm 5 0% B (0 min) 00% B ( 5 min) 0. ml/min (MPa) 7 C, 5 µl (0.N HCI) ESI (positive, m/z 0.) A: ACN/Formic acid (00/0.) B: 00mM Ammonium formate Intrada Amino Acid columns separate amino acid isomers quickly by using an optimized column length, as in the example of aminobutyric acid isomers (0Da) and leucine isomers (Da) using 00-50mm length columns. IMTAKT INTRADA AMINO ACID COLUMN ID 0 mm 0 mm 0 mm 50 mm 75 mm 00 mm 50 mm 50 mm.0 mm WAA0 WAA9 WAA WAA WAA WAA WAA5 WAA6.0 mm WAA0 WAA9 WAA WAA WAA WAA WAA5 WAA6.0 mm WAA WAA WAA WAA WAA5 WAA6 All of Imtakt stationary phases can also be made in the following internal diameters: 0.05 mm, mm, 0. mm, 0. mm, and 0.5 mm. 0 Chrom Tech your chromatography specialists

64 Imtakt Scherzo HPLC COLUMNS Scherzo C8 Multi-Mode Column The Scherzo C8 Family (SS-C8, SM-C8, SW-C8), consists of not only ODS ligands, but also anion ligands and cation ligands. It also provides reversed-phase modes, both ion exchange modes, and normal phase mode. Separation Mode Stationary Phase Properties Reversed Phase Octadecyl Increasing organic solvent composition (decreasing polarity of eluent) decreases retention. ODS + Anion Ligand + Anion Exchange + Cation Exchange + Cation Anion Increasing ionic strength (salt or acid concentration) decreases retention for acidic compounds. Generally, low ph increases retention. Increasing salt concentration decreases retention for basic compounds. SM-C8 retains more with increasing ph, while SS-C8 and SW-C8 retain more at lower ph. Normal Phase Anion/Cation Polar solutes which cannot be retained with 00% aqueous eluent may be retained by using > 50% organic solvent composition due to electrostatic interaction. ODS + Cation Ligand Three kinds of ODS with different ion exchange capacities IMTAKT SCHERZO SS-C8 COLUMN ID 0 mm 0 mm 0 mm 50 mm 75 mm 00 mm 50 mm 50 mm 500 mm 0.0 mm SS0P SS0P SS0P SS0P SS0P5 SS0P6 6.0 mm SS06 SS06 SS06 SS06 SS065 SS066.6 mm SS000 SS009 SS00 SS00 SS00 SS00 SS005 SS006 SS007.0 mm SS00 SS09 SS0 SS0 SS0 SS0 SS05 SS06.0 mm SS00 SS09 SS0 SS0 SS0 SS0 SS05 SS06.5 mm SS07 SS07 SS07 SS07 SS075 SS076.0 mm SS0 SS0 SS0 SS0 SS05 SS06 IMTAKT SCHERZO SM-C8 COLUMN ID 0 mm 0 mm 0 mm 50 mm 75 mm 00 mm 50 mm 50 mm 500 mm 0.0 mm SM0P SM0P SM0P SM0P SM0P5 SM0P6 6.0 mm SM06 SM06 SM06 SM06 SM065 SM066.6 mm SM000 SM009 SM00 SM00 SM00 SM00 SM005 SM006 SM007.0 mm SM00 SM09 SM0 SM0 SM0 SM0 SM05 SM06.0 mm SM00 SM09 SM0 SM0 SM0 SM0 SM05 SM06.5 mm SM07 SM07 SM07 SM07 SM075 SM076.0 mm SM0 SM0 SM0 SM0 SM05 SM06 SCHERZO SW-C8 COLUMN ID 0 mm 0 mm 0 mm 50 mm 75 mm 00 mm 50 mm 50 mm 500 mm 0.0 mm SW0P SW0P SW0P SW0P SW0P5 SW0P6 6.0 mm SW06 SW06 SW06 SW06 SW065 SW066.6 mm SW000 SW009 SW00 SW00 SW00 SW00 SW005 SW006 SW007.0 mm SW00 SW09 SW0 SW0 SW0 SW0 SW05 SW06.0 mm SW00 SW09 SW0 SW0 SW0 SW0 SW05 SW06.5 mm SW07 SW07 SW07 SW07 SW075 SW076.0 mm SW0 SW0 SW0 SW0 SW05 SW06 All of Imtakt stationary phases can also be made in the following internal diameters: 0.05 mm, mm, 0. mm, 0. mm, and 0.5 mm. Scherzo SS-C8 Large amount of strong ionic ligands loaded onto this ODS column. Effective for improved retention of zwitterions or weak ionic compounds. Scherzo SM-C8 Weak ionic ligands adequately loaded onto this ODS column. Designed for separation of basic/acidic compounds at neutral ph condition. Scherzo SW-C8 Low amount of strong ionic ligands loaded onto this ODS column. Effective for strong ionic compound elution or basic compounds with formic acid eluent. Authorized Distributor

65 HPLC COLUMNS Jordi Resolve Organic GPC Jordi Resolve 5 µm Organic GPC Columns No dislocations in pore distribution Clean light scattering background Highly symmetrical peaks Improved calibration Stable up to 0 C Low back pressure Monodisperse polymer particles 00% Divinylbenzene (DVB) Jordi Labs has recently developed a novel packing material for GPC systems. This column packing is based on 00% divinylbenzene providing enhanced mechanical stability and utilizes a new synthetic process which results in a monodisperse polymer column packing. It is well known that broad particle size distributions in particle based columns produces variations in packing density, lowers column resolution, reduces the column permeability and generates high back pressure. New generation Jordi GPC columns prepared with monodisperse 00% divinylbenzene particles with precisely controlled particle diameter and finely controlled pore structure provide high efficiency, high separation capacity and low back pressure with greater bed stability. Scanning electron micrographs and particle size distribution of 5 μm macroporous column packing material with 0 Å pore sizes are shown in Figure. The uniform size distribution and perfect spherical shapes are clearly seen. To maximize GPC resolution, the key is to use a column containing the maximum number of pores of the desired size to separate the molecular weight range of interest. New generation individual pore size Jordi Resolve columns 5 μm (7.8 mm ID x 00 mm L) provide high resolution in specific molecular weight ranges. Figure : SEM and particle size distribution of Jordi Resolve 5 μm column packing material. Figure : Resolve Mixed Bed Calibration Curve JORDI RESOLVE 5 µm ORGANIC GPC COLUMNS PART NO POROSITY PORE SIZE SIZE (mm) PRESSURE MW RANGE R Å 5 μm 7.8 x 00 8,000 PSI 00 0K R Å 5 μm 7.8 x 00 8,000 PSI 00 5K R507 0 Å 5 μm 7.8 x 00 8,000 PSI K R507 0 Å 5 μm 7.8 x 00,000 PSI 0K 600K R Å 5 μm 7.8 x 00,000 PSI 70K M R5075 Mixed Bed Low 5 μm 7.8 x 00,000 PSI K R5076 Mixed Bed Medium 5 μm 7.8 x 00,000 PSI 00 M The linear part of each calibration curve defines the molecular weight range of each individual porosity column. To cover a wider range of molecular weight with a constant resolution for the analysis of polydisperse or unknown materials, Jordi Labs designed mixed bed Resolve column 5 μm (7.8 mm ID x 00 mm L). Mixed bed Resolve column allows separation over the molecular weight range from 00 to,000,000 g/mol (PS equivalent) with a coefficient of determination (R) of 0.999, as shown in Figure, and an industry leading specification of 70,000 theoretical plates. In addition, high pore volume of Jordi Resolve column provides an increased resolution compared to the competitive column of the same particle size and length. Accurate blending of individual pore sizes and therefore, the wide molecular weight resolving range of Jordi Resolve column provides smooth peak shapes. Chrom Tech your chromatography specialists

66 Jordi Resolve Organic GPC HPLC COLUMNS Jordi Resolve µm Organic GPC Column µm particle optimized for the high temperature molecular weight analysis of polyethylene and other polyolefins For higher molecular weights, Jordi Labs offers its Jordi Resolve DVB μm columns. New generation Jordi Resolve columns are produced to allow separation over the molecular weight range from 500 to,000,000 g/mol (PS equivalent). Polyolefins often have broad molecular weight ranges characterized by high polydispersity values. Jordi Labs has developed mixed bed columns especially for these broad distribution samples which were designed by blending a large number of individual pore size packing materials. To maximize GPC resolution, the key is to use a column containing the maximum number of pores of the desired size to separate the molecular weight range of interest. New generation Jordi Resolve columns are produced to allow separation over the molecular weight range from 60 to,000,000 g/mol (PS equivalent) for μm particle size packing materials. Polyolefins often have broad molecular weight ranges characterized by high polydispersity values. Jordi Mixed bed μm columns have highly linear calibration curves with a coefficient of determination (R) of over the molecular weight range 60-,600,000 g/mol (PS equivalent). These columns have the ability to separate very broad samples without peak dislocations/shoulders for highly polydisperse samples. Analysis of NIST polyethylene standards including NIST 887, 75a and 76a along with industry samples of polypropylene, polyethylene and polystyrene have been performed to demonstrate column performance at elevated temperatures (60 ºC). The large particle size of the new Jordi μm column provides high efficiency and minimum shear degradation for high molecular weight polymers. Polyethylene samples analyzed in trichlorobenzene at 60 C are shown in Figure. Figure : SEM and particle size distributions of μm resin It is well known that broad particle size distributions in particle based columns produces variations in packing density, lowers column resolution, reduces the column permeability and generates high back pressure. New generation Jordi GPC columns prepared with monodisperse 00% divinylbenzene particles with precisely controlled particle diameter and finely controlled pore structure provide high efficiency, high separation capacity and low back pressure with greater bed stability. Scanning electron micrographs and particle size distributions of μm macroporous column packing materials with 0 Å pore sizes are shown in Figure. Figure : Chromatograms obtained with Resolve μm (7.8mm x 00mm) column for PE and related samples at 60 C. JORDI RESOLVE µm ORGANIC GPC COLUMNS PART NO POROSITY PORE SIZE SIZE (mm) PRESSURE MW RANGE R5077 Mixed Bed High μm 7.8 x 00,000 PSI 500 M Why Jordi Resolve Columns? Improved regularity Increased separation efficiency Highly linear calibration means increased accuracy Measures every MW between 500 and million What makes our new columns better? Monodisperse bead size Novel 00% DVB column packing Enhanced mechanical stability For what industries are these columns best suited? Petroleum Chemical Consumer Product Goods What are some common applications for Jordi Resolve Columns? High molecular weight polymers Polyethylene and Polypropylene at high temperature Authorized Distributor

67 HPLC COLUMNS Jordi Resolve Aqueous GPC Jordi Resolve Aqueous GPC Columns Jordi offers three column chemistries for GPC in aqueous mobile phases including: Jordi Resolve xstream Jordi Resolve Anion Jordi Resolve Cation Aqueous applications present a significant challenge in the field of size-based separations, due to the difficulty in preventing sample-column interactions. Jordi offers a wide selection of column packings to ensure success in your aqueous SEC separation. Jordi Resolve xstream columns allow for the separation of a wide range of samples in 00% aqueous, 00% organic or aqueous/organic mobile phases. Our Resolve Anion and Resolve Cation columns separate cationic and anionic samples based on charge-charge repulsions, without the need for high salt concentrations. Jordi Resolve xstream columns apply to a wide range of neutral, polar synthetic polymers and polysaccharides in mixed mobile phases, such as DMSO/HO. The extreme inertness of these packings extends their use to strongly basic mobile phases, including M NaOH, that would destroy the competitors methacrylate-based products. The Jordi Resolve xstream column packing is an exciting breakthrough technology, which has increased hydrophilicity to reduce sample-column interactions. This novel resin features 00% polymeric polyamide chemistry. The Jordi Resolve xstream column, unlike typical GPC columns, performs well in any range of mobile phases, including 00% aqueous, 00% organic or any mixture of solvents. This new column is optimized for use in aqueous-based mobile phases and has the unique ability to separate a variety of cationic and polar polymers. Dextrans, polysaccharides and vinyl ether/maleic acid copolymers prove to separate well on the Jordi Resolve xstream in pure water. Separations in THF include, but are not limited to, phenoxy resins, poly(n-butyl methacrylate), polycaprolactone, several styrenic polymers, PMMA and other methacrylic polymers. The Jordi Resolve xstream is also appropriate for analysis in HFIP, eliminating sample-column interactions and providing excellent resolution in the separation of nylons and PET. Other organic mobile phases applicable to separations on the Jordi Resolve xstream include chloroform, DMSO, DMAC and DMF. Jordi Resolve Anion and Resolve Cation columns apply to the separation of charged polymers without the need for high salt concentrations. These columns are an excellent choice for light scattering analyses, where high salt concentrations compromise system performance. Jordi Anion columns have a negatively charged surface for the separation of anionic polymers, such as poly(styrene sulfonate). Typical solvents for this phase include aqueous/organic mixtures, such as water/methanol. Jordi Resolve Cation columns have a tertiary amine group, which in weakly acidic mobile phases coverts to the positively charged quaternary amine. Common applications include the separation of amine polymers, such as poly(ethyleneimine) in water/acetic acid solutions. The ordering table contains the typical MW ranges for Jordi Resolve aqueous columns. The range is dependent on sample and mobile phase. JORDI RESOLVE AQUEOUS GPC COLUMNS PART NO PHASE POROSITY PARTICLE SIZE SIZE (mm) PRESSURE MW RANGE R06 xstream 500Å 5 μm 7.8 x 00 8,000 PSI 00 5K R066 xstream Mixed Bed 5 μm 7.8 x 00,000 PSI 5K M R570 Cation 500Å 5 μm 7.8 x 00 8,000 PSI 00 5K R5706 Cation Mixed Bed 5 μm 7.8 x 00,000 PSI 00 M R57 Anion 500Å 5 μm 7.8 x 00 8,000 PSI 00 5K R576 Anion Mixed Bed 5 μm 7.8 x 00,000 PSI 00 M Monodisperse polymer particles 00% Divinylbenzene (DVB) Broad pore size range Features No dislocations in pore distribution Clean light scattering background Highly symmetrical peaks Improved calibration Low back pressure Stable up to 0 C Long column life Repeatability Plate count Resolution Why Jordi Resolve Columns? Improved regularity Increased separation efficiency Highly linear calibration means increased accuracy Measures every MW between 500 and million What makes our new columns better? Monodisperse bead size Novel 00% DVB column packing Enhanced mechanical stability For what industries are these columns best suited? Petroleum Chemical Consumer Product Goods What are some common applications for Jordi Resolve Columns? High molecular weight polymers Polyethylene and Polypropylene at high temperature Chrom Tech your chromatography specialists

68 Perkin Elmer Brownlee HPLC COLUMNS Perkin Elmer Brownlee Columns Aquapore is a wide-pore (00Å, 7 μm), silica based support suitable for the separation of large biopolymers. Spheri-5 is a small pore (80Å, 5 μm), silica based sorbent for separating small molecules. Both monofunctional and polyfunctional C8 and C8 sorbents are available. Polypore packings are derivatized polystyrene divinylbenzene copolymer resins. The Polypore H cartridges are optimized for organic acid separations and the Polypore CA cartridges are for saccharide separations. BROWNLEE NEWGUARD CARTRIDGE COLUMNS. mm ID x.5 cm PART NO DESCRIPTION TYPE QTY Aquapore Dimethyl, 7 μm RP- /pk Aquapore Butyl, 7 μm RP- /pk Aquapore Octyl, 7 μm RP-8 /pk Aquapore ODS, 7 μm RP-8 /pk Aquapore Phenyl, 7 μm Phenyl /pk Aquapore Amino, 7 μm Amino /pk Aquapore Cyano, 7 μm Cyano /pk Aquapore AX-00, 7 μm Anion /pk Aquapore Diol, 7 μm Diol /pk Aquapore Silica, 7 μm Silica /pk 0-7 Validated C8, 5 μm C8 /pk NewGuard Holder, Holds a single NewGuard ea cartridge for use with conventional HPLC columns Union for direct coupling of two cartridges ( Holder bodies and end assemblies also required) ea SPECIFICATIONS PRODUCT FUNCTIONALITY PORE SIZE SURFACE AREA % CARBON END CAP Spheri-5, RP-8 Octyl (C8) monofunctional 80 Å 80 m/g 6% yes Spheri-5, RP-8 Octyl (C8) monofunctional 80 Å 80 m/g % yes Spheri-5, ODS Octyl (C8) polyfunctional 80 Å 80 m/g % yes Spheri-5, Phenyl Phenyl 80 Å 80 m/g 6% yes Spheri-5, Silica Hydroxyl 80 Å 80 m/g N/A N/A Spheri-5, Amino Aminopropyl 80 Å 80 m/g % no Spheri-5, Cyano Cyanopropyl 80 Å 80 m/g % no Aquapore ODS, Octadecyl (C8) 00 Å 00 m/g 0% yes OD-00 Aquapore Octyl, Octyl (C8) 00 Å 00 m/g 5% yes RP-00 Aquapore Butyl, Butyl (C) 00 Å 00 m/g % yes BU-00 Aquapore weak anion, AX-00 Polyethyleneimine 00 Å 00 m/g N/A N/A Polypore H Hydrogen form microporous Polypore CA Calcium form microporous PERKIN ELMER BROWNLEE HPLC CARTRIDGE COLUMNS REVERSE PHASE POLAR PHASE ION EXCHANGE CM CARTRIDGES (/PK) REQUIRES HOLDER CM CARTRIDGES REQUIRES HOLDER CM CARTRIDGES REQUIRES HOLDER DESCRIPTION.6 mm ID. mm ID.6 mm ID. mm ID.6 mm ID. mm ID RP-8 Spheri-5, C-8, 5 μm, monofunctional RP-8 Spheri-0, C-8 0 μm, monofunctional Pecosphere CR, C8, µm, base deactivated RP-8 Spheri-5, C-8 5 μm, monofunctional RP-8 Spheri-0, C-8 0 μm, monofunctional ODS Spheri-5, C-8 5 μm, polyfunctional Phenyl Spheri-5 5 μm Cyano Spheri-5, 5 μm, Cyanopropyl OD-00 Aquapore ODS, 7 μm, 00 Å pore size RP-00 Aquapore Octyl, 7 μm, 00 Å pore size BU-00 Aquapore Butyl, 7 μm, 00 Å pore size Silica Spheri-5, 5 μm, 80 Å pore size Silica Spheri-0 0 μm, 80 Å pore size Amino Spheri-5, 5 μm, Aminopropyl Cyano Spheri-5, Cyanopropyl AX-00 Aquapore Weak Anion 00 Å, 7 μm Hydrogen form Polypore H, 0 μm Calcium form Polypore CA, 0μm Holder

69 HPLC COLUMNS Regis Chiral Stationary Phases Regis Pirkle Chiral HPLC Columns Whelk-O Analytical to Preparative Columns The Whelk-O is useful for the separation of underivatized enantiomers in a number of families including amides, epoxides, esters, ureas, carbamates, ethers, aziridines, phosphonates, aldehydes, ketones, carboxylic acids, alcohols, and non-steroidal anti-inflammatory drugs (NSAIDs). This π-electron acceptor/ π-electron donor phase exhibits an extraordinary degree of generality. The broad versatility observed on the Whelk-O column compares favorably with polysaccharide-derived chiral stationary phases. In addition, because Whelk-O is covalently bonded to the support, the phase is compatible with all commonly used mobile phases, including aqueous systems a distinct advantage over polysaccharide derived chiral stationary phases. Other advantages include column durability, excellent efficiency, ability to invert elution order, and excellent preparative capacity. SIZE (mm) PARTICLE SIZE (µm) (S,S) (R,R).6 x x x x x x x x x x x x x x x SIZE (mm) PARTICLE SIZE (µm) (S,S) (R,R) 50 x x x x x x x x x x x x x x x x x x x x x x x x x x x Bulk packaging is available for 5 µm, 0 µm, and 6 µm material Bulk packaging and select sizes of 0 µm material are available with YMC silica Select sizes are also available with Exsil silica 6 Chrom Tech your chromatography specialists

70 Regis Chiral Stationary Phases HPLC COLUMNS RegisPack Columns RegisPack polysaccharide coated chiral columns are made using a unique production process of coating the proven chiral selector tris-(,5-dimethylphenyl) carbamoyl amylose on high purity silica gel. RegisPack columns are available in, 5, 0, and 0 micron particle sizes enabling easy scale up from analytical to preparative using HPLC or SFC conditions. Bulk material is available upon request. SIZE (mm) PARTICLE SIZE (µm) PART NO.6 x x x x x x x x x x x x x x x x x x x x SIZE (mm) PARTICLE SIZE (µm) PART NO.6 x x x x x x x x x x x Other Regis Phases Available Regis Pirkle-Type Chiral HPLC Columns Whelk-O Leucine Phenylglycine Beta-GEM Alpha-Burke Pirkle -J DACH-DNB ULMO Crown Ether Chiral Stationary Phases ChiroSil RCA (+) ChiroSil SCA (-) Polysaccharide Coated Chiral Stationary Phases RegisCell RegisPack CLA- Authorized Distributor

71 HPLC COLUMNS Regis Immobilized Artificial Chromatography Regis Immobilized Artificial Chromatography Immobilized Artificial Membrane (IAM) technology is an innovative approach to chromatography in which the chromatographic surface emulates the lipid environment of the cell membrane., HPLC Separation Tools for Membrane Protein Purification and Drug Membrane Permeability Prediction Phosphatidylcholine (PC) is the major phospholipid found in cell membranes. IAM chromatography phases prepared from PC analogs closely mimic the surface of a biological cell membrane. Consequently, IAM phases display a high affinity for membrane proteins and are useful in membrane protein purification and in the study of drug membrane interactions. The IAM surface is formed by covalently bonding the membrane-forming phospholipids to silica. Several different types of IAM columns are used for various applications. Membrane Protein Purification IAM.PC IAM.PC.MG Drug Discovery IAM.PC.DD IAM Fast-Screen Mini Columns Regis IAM.PC The IAM.PC phase, developed by Dr. Charles Pidgeon of Purdue University, was the first in a line of IAM phases to be manufactured by Regis. Use of this phase has simplified the inherent difficulties of protein isolation and purification, -9 allowing for rapid purification of membrane proteins while maintaining biological activity. The IAM.PC phase is an important tool for the pharmaceutical industry and academia alike. The first IAM stationary phase was based on the prevalent membrane lipid, phosphatidylcholine (PC), and consists of monolayers of amphiphilic phospholipids covalently bonded to aminopropyl silica particles through a terminal amide linkage. As a result, the bulky phosphatidylcholine groups shield many of the amine binding sites on the silica surface, preventing amine interaction with the protein molecules. The membrane nature of the IAM phase imparts surface characteristics which are useful in the chromatography of membrane proteins. These include: high protein loading, increased protein recovery, recovery of functional activity, and selectivity for membrane proteins. Large membrane proteins can interact with any combination of polar headgroup, hydrophobic chain, or inner amine groups. The subsurface has been shown to interact with certain solutes, and may or may not contribute to the separation of a given biomolecule. The residual amines can be left unaltered on the subsurface or deactivated through an endcapping procedure, which results in increased stability of the bonded phase. The methyl glycolate endcapping, for example, converts residual amines to neutral amides and introduces a hydroxyl group (IAM.PC.MG). APPLICATIONS Purification of Cytochrome P50, Isolation of membrane proteins, Prediction of solute transport across human skin, Prediction of amino acid transport across the blood-brain barrier, Binding of solutes to liposome membranes, Immobilization of Trypsin and chymotrypsin for the determination of their inhibitor and substrate activity DESCRIPTION PARTICLE SIZE SIZE (mm x mm) PART NO IAM.PC 0 μm, 00Å.6 x IAM.PC 0 μm, 00Å.6 x IAM.PC Guard Kit* 0 μm, 00Å.0 x IAM.PC.Guard Replacement** 0 μm, 00Å.0 x IAM.PC.MG 0 μm, 00Å.6 x IAM.PC.MG 0 μm, 00Å.6 x IAM.PC.MG Guard Kit* 0 μm, 00Å.0 x * Includes holder and guard cartridges ** Includes guard cartridges 8 Chrom Tech your chromatography specialists

72 Regis Immobilized Artificial Chromatography HPLC COLUMNS Regis Immobilized Artificial Chromatography (cont.) Regis IAM.PC.DD IAM Fast-Screen Mini Column IAM chromatography has recently gained acceptance among drug discovery chemists for estimating the membrane permeability of small molecule drugs. The figure to the right illustrates that the interaction between membrane bilayer and drug can be modeled by the IAM column/drug system. K IAM, the equilibrium constant describing the relative concentrations of drug in the membrane and in the external fluid, is analogous to the K IAM. This IAM technique provides superior correlation with experimentally determined drug permeability when compared to other chromatographic methods. ODS silica, for example, retains analytes solely on the basis of hydrophobicity. IAM more closely mimics the interaction of analytes with biological membranes, where a combination of hydrophobic, ion pairing, and hydrogen bonding interactions are possible. This combination of interactions measured by the IAM column is known as phospholipophilicity. DESCRIPTION PARTICLE SIZE SIZE (mm x mm) PART NO IAM.PC.DD 0 μm, 00Å.6 x IAM.PC.DD 0 μm, 00Å.6 x IAM.PC.DD 0 μm, 00Å.6 x IAM.PC.DD 0 μm, 00Å.0 x IAM.PC.DD Guard Kit* 0 μm, 00Å.0 x IAM.PC.DD Guard Replacement** 0 μm, 00Å.0 x * Includes holder and guard cartridges ** Includes guard cartridges Fluid membrane bilayer can be modeled by IAM column. DESCRIPTION PARTICLE SIZE SIZE (mm x mm) PART NO IAM Fast-Screen Mini Column Kit (Includes: columns, cartridge holder, care & use booklet) IAM Fast-Screen Mini Columns, 6/pk (Includes 6 columns, care & use booklet) IAM Fast Screen Mini Columns, /pk (Includes columns, care & use booklet) 0 μm, 00Å.0 x μm, 00Å.0 x μm, 00Å.0 x IAM References. Pidgeon, C.; et al.; IAM Chromatography: An in vitro Screen for Predicting Drug Membrane Permeability; J. Med. Chem. 995, 8, Ong, S.; et al.; Thermodynamics of Solute Partitioning into Immobilized Artificial Membranes; Anal. Chem. 995, 67, Pidgeon, C.; U.S. Patent 9 98, Pidgeon, C.; U.S. Patent , Pidgeon, C.; Venkatarum, U. V.; Immobilized Artificial Membrane Chromatography: Supports Composed of Membrane Lipids; Anal. Biochem. 989, 76, Stevens, J. M.; et al.; Characterization of Immobilized Artificial Membrane HPLC Columns Using Deoxy Nucleotides as Model Compounds; Biochromatography989,, Markovich, R. J.; et al.; Fourier Transform Infrared Assay of Membrane Lipids Immobilized to Silica: Leaching and Stability of Immobilized Artificial Membrane-Bonded Phases; Anal. Biochem. 989, 8, Pidgeon, C.; Solid Phase Membrane Mimetics: Immobilized Artificial Membranes; Enz. Microb. Technol. 990,, Markovich, R. J.; et al.; Silica Subsurface Amine Effect on the Chemical Stability and Chromatographic Properties of End-Capped Immobilized Artificial MembraneSurfaces; Anal. Chem. 99, 6, Artursson, P.; Karlsson, J.; Correlation Between Oral Drug Adsorption in Humans and Apparent Drug Permeability Coefficients in Human Intestinal Epithelial (Caco-) Cells; Biochem. Biophys. Res. Commun. 99, 75, Schanker, L. S.; J. Pharmacol. Exp. Ther. 958,, TECH TIP Because the IAM Fast-Screen Mini Column is inexpensive, has a very short analysis time, and provides drug permeability estimates for hundreds of drug candidates in a fraction of the time of conventional methods, the IAM Fast-Screen Mini Column becomes the economical alternative for high throughput screening. Authorized Distributor

73 HPLC COLUMNS Restek Raptor Restek Raptor Superficially Porous Core-Shell LC Columns Biphenyl (USP L) Ideal for bioanalytical applications like drug and metabolite analyses Heightened selectivity and retention for compounds that are hard to resolve or elute early on C8 and other phenyl chemistries Limits ionization suppression; allows MS-friendly mobile phases CH Si CH O ARC-8 Ideal for high-throughput LC-MS/MS applications with minimal sample prep Well-balanced retention profile for better detection and integration of large, multiclass analyte lists Sterically protected to endure low-ph mobile phases without sacrificing retention or peak quality Part of Restek s Raptor LC column line featuring.7 and 5 μm SPP core-shell silica With Raptor LC columns, Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or "core-shell" particles) with the resolution of highly selective USLC technology. This new breed of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation. The Raptor ARC-8 column features a well-balanced retention profile without the drawbacks of using an ordinary C8 in the harsh, acidic mobile phases needed for mass spectrometry (MS). Even after extended use in these low-ph (.0) conditions, the sterically protected ARC-8 offers consistent retention, peak shape, and response for charged bases, neutral acids, small polar compounds, and more. For the rapid analysis of large, multiclass assays by LC-MS/MS, the acid-resistant Raptor ARC-8 truly is ahead of the curve. C8 (USP L) Compatible with moderately acidic to neutral mobile phases (ph 8) Excellent data quality in food, environmental, bioanalytical, and other applications General purpose column for reversed phase chromatography Increased retention of hydrophobic compounds When you need a general-purpose LC column, don't just grab any C8. Choose the speed, efficiency, and long-lasting ruggedness of the Raptor C8. This traditional end-capped C8 offers the highest hydrophobic retention of any Raptor phase, and it is compatible with a wide range of mobile phases from moderately acidic to neutral (ph 8). Whether for food safety or environmental or bioanalytical analyses, this phase offers consistently excellent data quality in less time across a myriad of reversed-phase applications, matrices, and compound classes. To lower costs and improve profitability, you need columns to last longer, data to be reproducible, and existing HPLC instrumentation to run faster get there with the Raptor C8. TMS Si O TMS 0 Chrom Tech your chromatography specialists

74 Restek Raptor HPLC COLUMNS Restek Raptor Superficially Porous Core-Shell LC Columns (cont.) RESTEK RAPTOR SPECIFICATIONS BIPHENYL ARC-8 C8 Phase Phenyl (L) C8, octadecylsilane (L) C8, octadecylsilane (L) Ligand type Biphenyl Sterically protected C8 End-capped C8 Pore size 90 Å 90 Å 90 Å Max temp 80 C 80 C 80 C ph range Particle Surface area Max pressure.7 µm or 5 µm superficially porous silica (SPP or "core-shell") 50 m/g (.7 µm) 00 m/g (5 µm) 600 bar / 8,700 PSI (.7 µm) 00 bar / 5,800 PSI (5 µm).7 µm or 5 µm superficially porous silica (SPP or "core-shell").7 µm or 5 µm superficially porous silica (SPP or "core-shell") 50 m/g (.7 μm) 00 m/g (5 μm) 50 m/g (.7 μm) 00 m/g (5 μm) 600 bar / 8,700 PSI (.7 µm) 00 bar / 5,800 PSI (5 µm) 600 bar / 8,700 PSI (.7 µm) 00 bar / 5,800 PSI (5 µm) SIZE (mm) PARTICLE SIZE (µm) BIPHENYL ARC-8 C8.6 x 50 5 RTK RTK-9575 RTK x 50 5 RTK RTK-9565 RTK x 00 5 RTK RTK-955 RTK x 50 5 RTK RTK-9555 RTK x 5 Guard, /pk RTK RTK RTK x 50 5 RTK-90956E RTK-956E RTK-9056E.0 x 00 5 RTK-9095E RTK-95E RTK-905E.0 x 50 5 RTK-90955E RTK-955E RTK-9055E.0 x 0 5 RTK-9095E RTK-95E RTK-905E.0 x 5 Guard, /pk RTK RTK-9505 RTK x 50 5 RTK RTK-956 RTK x 00 5 RTK-9095 RTK-95 RTK-905. x 50 5 RTK RTK-955 RTK x 5 Guard, /pk RTK RTK-9505 RTK x 50.7 RTK-909A65 RTK-9A65 RTK-90A65.6 x 00.7 RTK-909A5 RTK-9A5 RTK-90A5.6 x 50.7 RTK-909A55 RTK-9A55 RTK-90A55.6 x 0.7 RTK-909A5 RTK-9A5 RTK-90A5.6 x 5 Guard, /pk RTK-909A050 RTK-9A050 RTK-90A050.0 x 50.7 RTK-909A6E RTK-9A6E RTK-90A6E.0 x 00.7 RTK-909AE RTK-9AE RTK-90AE.0 x 0.7 RTK-909AE RTK-9AE RTK-90AE.0 x 50.7 RTK-909A5E RTK-9A5E RTK-90A5E.0 x 5 Guard, /pk RTK-909A05 RTK-9A05 RTK-90A05. x 50.7 RTK-909A6 RTK-9A6 RTK-90A6. x 00.7 RTK-909A RTK-9A RTK-90A. x 50.7 RTK-909A5 RTK-9A5 RTK-90A5. x 0.7 RTK-909A RTK-9A RTK-90A. x 5 Guard, /pk RTK-909A05 RTK-9A05 RTK-90A05 Raptor EXP Guard Column Cartridges Free-Turn architecture lets you change cartridges by hand without breaking inlet/outlet fluid connections no tools needed Guard column cartridges require EXP direct connect holder EXP Repl Ferrule CHROM TECH RELATED PRODUCTS EXP Nut & Ferrule Hand-tight to 8,700 PSI Wrench-tight to 0,000 PSI Authorized Distributor

75 HPLC COLUMNS Restek Raptor Restek Raptor Chromatograms Get Complete Isobaric Resolution and Sub-5-minute Pain Panel Runs Column: Raptor Biphenyl RTK-909A5E.0 x 50 mm,.7 µm Instrumentation: API LC-MS/MS Detector: AB SCIEX API000 MS/MS; ESI+ Mobile Phase: A: 0.% formic acid in H 0 B: 0.% formic acid in methanol Gradient: Temp: 0 C Flow Rate: 0% B, 0.00 min; 5% B,.50 min; 00% B,.50 min; 00% B,.70 min; 0% B,.7 min; 0% B, 5.00 min 0.6 ml/min XIC of isobars.0.5 Excellent separation of isobaric compounds Time (min) Analytes separated from early-eluting matrix. Human Urine Matrix Components. Morphine. Oxymorphone. Hydromorphone. Amphetamine 5. Methamphetamine 6. Codeine 7. Oxycodone 8. Hydrocodone 9. Norbuprenorphine 0. Meprobamate. Fentanyl. Buprenorphine. Flurazepam. Sufentanil 5. Methadone 6. Carisoprodol 7. Lorazepam 8. Diazepam Sample: Diluent: urine:mobile phase A: mobile phase B (7:76:7) Injection: 0 µl Time (min) Concentration: 0 00 ng/ml Lorazepam was prepared at 00 ng/ml; all other analytes are 0 ng/ml Analysis of Vitamin D and Metabolites with Raptor ARC-8 Column: Raptor ARC-8 RTK-9A. x 00 mm,.7 µm Instrumentation: Shimadzu UFLCXR Detector: Mobile Phase: ABSCIEX API000 ; Ion Source: TurbolonSpray ; Ion Mode: ESI+ A: 0.% formic acid + FmM ammonium formate in water B: 0.% formic acid + 5 mm ammonium formate in methanol..5-dihydroxyvitamin D. 5-Hydroxyvitamin D. 5-Hydroxyvitamin D. Vitamin D 5. Vitamin D Gradient (%B): 0.00 min (90%),.00 min (00%),.0 min (90%), 6.00 (90%) Temp: 0 C Flow Rate: 0.5 ml/min Sample: Diluent: Methanol Injection: 5 µl. Concentration: 00 ng/ml Separating fat-soluble vitamins by LC can be time consuming. The Raptor ARC-8 column, however, can analyze these difficult compounds using reversed-phase chromatography (RPC) in less time than traditional columns to increase productivity. Plus in the bioanalytical arena, the ARC-8 can quantitate the metabolites of vitamin D using the same column and mobile phases. Chrom Tech your chromatography specialists

76 Restek Raptor HPLC COLUMNS Aldehyde-Ketone-DNPH TO-A Calibration Mix on Raptor C8 Column A: Mobile Phase: Temperature: 0 C Detector: Raptor C8 RTK-90A65 50 mm x.6 mm ID A: Water B: Methanol: acetonitrile (650:50 65,.8 nm Formaldehyde. Acetaldehyde. Acrolein. Acetone 5. Propionaldehyde 6. Crotonaldehyde 7. Butyraldehyde 8. Benzaldehyde 9. Isovaleraldehyde 0. Valeraldehyde. o-tolualdehyde. m-tolualdehyde. p-tolualdehyde. Hexanal 5.,5-Dimethylbenzaldehyde Time (min) Cannabis Potency by LC--Cannabis Sample 7 on Raptor ARC-8 Column A: Mobile Phase: Temperature: 50 C Raptor ARC-8 RTK-9A65 50 mm x.6 mm ID 0.% Formic acid in water 0.% Formic acid in acetonitrile 5 6. Cannabidiolic acid. Cannabigerol. Cannabinol. delta-9-tetrahydrocannabinol 5. Cannabichromene 6. delta-9-tetrahydrocannabinolic acid A Detector: 0 nm Time (min) Cannabis Potency by LC--Cannabis Sample 7 on Raptor ARC-8 Column A: Raptor Biphenyl RTK-909A55 50 mm x.6 mm ID Mobile Phase: Water:Methanol (50:50) Flow: Temperature: 0 C. ml/min. Benzene. Nitrobenzene.,,5-Trinitrobenzene.,-Dinitrotoluene Detector: Waters Acquity 5 nm Time (min) Authorized Distributor

77 HPLC COLUMNS Restek Ultra Restek Ultra HPLC Columns RESTEK LC ANALYTICAL COLUMN SPECIFICATIONS ULTRA AQUEOUS C8 ULTRA IBD ULTRA BIPHENYL ULTRA PFP PROPYL ULTRA C8 Particle size: µm or 5 µm, spherical µm or 5 µm, spherical µm or 5 µm, spherical µm or 5 µm, spherical µm or 5 µm, spherical Pore size: 00 Å 00 Å 00 Å 00 Å 00 Å Carbon load: 5% % 5% % 0% End-cap: no no yes yes yes ph range:.5 to 8.5 to 8.5 to 8.5 to 8.5 to 8 Temperature limit: 80 C 80 C 80 C 80 C 80 C USP phase code: L L68 L L L Phase category: modified C8 polar-embedded alkyl phenyl fluorophenyl propyl C8, octadecylsilane Ligand type: proprietary polar modified and functionally bonded C8 proprietary polar functional embedded alkyl unique Biphenyl pentafluorophenyl propyl monomeric C8 Ultra Aqueous C8 (USP L) Ultra PFP Propyl (USP L) The Restek Aqueous C8 is a rugged, reversed-phase column with a well-balanced retention profile. It can effectively retain more types of solutes than a conventional C8 and is ideal for multicomponent LC-MS analyses. The general-purpose Aqueous C8 boasts high reproducibility and compatibility with many mobile phase conditions even 00% aqueous. And when used with a gradient, it eliminates the all-too-common issue of multiple compounds eluting near the column void time. Si O O P The Restek PFP Propyl is a great choice for the retention and selectivity of charged bases, electronegative compounds, and amine-containing compounds. Unlike a conventional cyano column, the Restek PFP Propyl is much more amenable to LC-MS because it is more reliable and efficient with acidic mobile phases. This versatile column is also compatible with highly aqueous mobile phases and HILIC separations. F F F CH Si CH O F F Ultra IBD (USP L68) The Restek IBD is a polar-embedded column that acts as a strong hydrogen bonder and may be the most versatile column available today. With a unique polar group, this column is very retentive and selective for acids. It also provides symmetrical peak shape for strong bases. Restek's IBD is compatible with 00% aqueous mobile phases and can be used under reversed-phase or HILIC conditions to retain very polar, ionic compounds in highly organic mobile phases. P CH Si CH O Ultra C8 (USP L) The general-purpose Restek C8 is a conventional monomeric octadecylsilane column suitable for analyses of a wide range of compounds from acidic through slightly basic. Steroid Panel Analysis on the Ultra Biphenyl 5 5 Ultra Biphenyl (USP L) Since 005, the Restek Biphenyl has offered a greater degree of dispersion than conventional phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and a greater range of usability. Because of these heightened interactions, this column shows substantial increases in retention especially for dipolar, unsaturated, or conjugated solutes and enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing sensitivity and selectivity in LC-MS analyses. CH Si CH O 6. Cortisol Estradiol. -Deoxycortisol. DHEA 5. Boldenone 6. Corticosterone 7. Stanozolol 8. Epitestosterone 9. 7-OH 0. Testosterone. Trenbolone. Methyltestosterone. Nandrolone Hydroxyvitamin D 5. Androstenedione 6. Progesterone 6 Chrom Tech your chromatography specialists Time (min)

78 Restek Ultra HPLC COLUMNS Restek Ultra HPLC Columns (cont.) SIZE (mm) PARTICLE SIZE (µm) ULTRA AQUEOUS C8 ULTRA IBD ULTRA BIPHENYL ULTRA PFP PROPYL ULTRA C8.6 x 50 5 RTK RTK RTK RTK RTK x 50 5 RTK RTK RTK RTK RTK x 00 5 RTK RTK RTK RTK RTK x 50 5 RTK RTK RTK RTK RTK x 0 5 RTK RTK RTK RTK RTK x 50 5 RTK-97857E RTK-97557E RTK-90957E RTK-97957E RTK-9757E.0 x 50 5 RTK-97856E RTK-97556E RTK-90956E RTK-97956E RTK-9756E.0 x 00 5 RTK-9785E RTK-9755E RTK-9095E RTK-9795E RTK-975E.0 x 50 5 RTK-97855E RTK-97555E RTK-90955E RTK-97955E RTK-9755E.0 x 0 5 RTK-9785E RTK-9755E RTK-9095E RTK-9795E RTK-975E. x 50 5 RTK RTK RTK RTK RTK x 50 5 RTK RTK RTK RTK RTK x 00 5 RTK-9785 RTK-9755 RTK-9095 RTK-9795 RTK-975. x 50 5 RTK RTK RTK RTK RTK x 0 5 RTK-9785 RTK-9755 RTK-9095 RTK-9795 RTK x 50 5 RTK RTK RTK RTK RTK x 50 5 RTK RTK RTK RTK RTK x 00 5 RTK-9785 RTK-9755 RTK-9095 RTK-9795 RTK x 50 5 RTK RTK RTK RTK RTK x 0 5 RTK-9785 RTK-9755 RTK-9095 RTK-9795 RTK x 50 RTK RTK RTK RTK RTK x 00 RTK-9785 RTK-9755 RTK-9095 RTK-9795 RTK x 50 RTK RTK RTK RTK RTK x 0 RTK-9785 RTK-9755 RTK-9095 RTK-9795 RTK x 50 RTK-9786E RTK-9756E RTK-9096E RTK-9796E RTK-976E.0 x 00 RTK-978E RTK-975E RTK-909E RTK-979E RTK-97E.0 x 50 RTK-9785E RTK-9755E RTK-9095E RTK-9795E RTK-975E.0 x 0 RTK-978E RTK-975E RTK-909E RTK-979E RTK-97E. x 50 RTK-9786 RTK-9756 RTK-9096 RTK-9796 RTK-976. x 00 RTK-978 RTK-975 RTK-909 RTK-979 RTK-97. x 50 RTK-9785 RTK-9755 RTK-9095 RTK-9795 RTK-975. x 0 RTK-978 RTK-975 RTK-909 RTK-979 RTK-97.0 x 50 RTK-9786 RTK-9756 RTK-9096 RTK-9796 RTK x 00 RTK-978 RTK-975 RTK-909 RTK-979 RTK-97.0 x 50 RTK-9785 RTK-9755 RTK-9095 RTK-9795 RTK x 0 RTK-978 RTK-975 RTK-909 RTK-979 RTK-97 CHROM TECH RELATED PRODUCTS EXP Repl Ferrule EXP Nut & Ferrule Hand-tight to 8,700 PSI Wrench-tight to 0,000 PSI RTK-995 UltraShield UHPLC precolumn filter, 0.5 µm Authorized Distributor

79 HPLC COLUMNS Restek Force Restek Force HPLC Columns Force FluoroPhenyl (USP L) Force Biphenyl (USP L) Capable of both reversed-phase and HILIC separations Ideal for increasing sensitivity and selectivity in LC-MS analyses Offers increased retention for charged bases F F F F F CH Si CH O Ideal for bioanalytical testing applications like drug and metabolite analyses Heightened selectivity and retention for compounds that are hard to resolve or elute early on C8 and other phenyl chemistries CH Si CH O Force C8 (USP L) Limits ionization suppression and allows simple, MS-friendly mobile phases Restek s most popular LC stationary phase A traditional end-capped C8 ideal for generalpurpose use in reversed-phase chromatography Wide ph range ( 8) provides excellent data quality for many applications, matrices, and compounds Offers high hydrophobic retention TMS Si O TMS SIZE (mm) PARTICLE SIZE (µm) BIPHENYL C8 FLUOROPHENYL.6 x 50 5 RTK x 50 5 RTK RTK RTK x 00 5 RTK RTK-9655 RTK x 50 5 RTK RTK x 50 5 RTK-96956E RTK-9656E RTK-96956E x 00 5 RTK-9695E RTK-965E RTK-9695E x 50 5 RTK-96955E RTK-9655E RTK-96955E. x 50 5 RTK RTK-9656 RTK x 00 5 RTK-9695 RTK-965 RTK x 50 5 RTK RTK-9655 RTK x 50 RTK RTK-9665 RTK x 00 RTK-9695 RTK-965 RTK x 5 Guard, /pk RTK RTK RTK x 50 RTK-9696E RTK-966E RTK-9696E x 00 RTK-969E RTK-96E RTK-969E x 50 RTK-9695E RTK-965E RTK-9695E x 5 Guard, /pk RTK RTK RTK x 50 RTK-9696 RTK-966 RTK x 00 RTK-969 RTK-96 RTK-969. x 50 RTK-9695 RTK-965 RTK x 0 RTK-969 RTK-96 RTK-969. x 5 Guard, /pk RTK RTK RTK x 00.8 RTK-969E RTK-96E RTK-969E x 50.8 RTK-9695E RTK-965E RTK-9695E. x 00.8 RTK-969 RTK-96 RTK-969. x 50.8 RTK-9695 RTK-965 RTK x 0.8 RTK-969 RTK-96 RTK-969 ULTRASHIELD UHPLC PRECOLUMN FILTERS PART NO PORE SIZE QTY RTK µm ea RTK µm 5/pk RTK µm 0/pk RTK µm ea RTK µm 5/pk RTK µm 0/pk RTK EXP Repl Ferrule CHROM TECH RELATED PRODUCTS EXP Nut & Ferrule Hand-tight to 8,700 PSI Wrench-tight to 0,000 PSI 6 Chrom Tech your chromatography specialists

80 C Restek Roc HPLC COLUMNS Restek Roc HPLC Columns Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 00 bar HPLC system Solid and reliable delivers the peak shape, reproducibility, ruggedness, and performance you demand Exceptional value for routine analyses and large-volume workflows high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods Ideal for a wide range of applications from food to pharma Solid, reliable Roc HPLC columns are pressure rated for any 00 bar HPLC system and deliver the peak shape, reproducibility, ruggedness, and performance you demand for all of your conventional HPLC applications, especially USP and other compendial methods. An exceptional value for routine analyses and large-volume workflows, Roc columns are made from high-purity, fully porous silica backed by quality manufacturing and are available in a variety of phases. Just 5 phases cover 98% of USP methods. Newly designed minimal packaging offers compact storage with serial #-specific certificates of analysis available upon request. C8 C8 PHENYL-HEXYL CYANO SILICA N CH Si CH O CH Si CH O CH Si CH O CH Si O CH OH Si O O O USP Phase Code L L7 L L0 L Stationary Phase Category C8, octadecylsilane C8, octylsilane phenyl cyano bare silica Ligand Type monomeric C8 monomeric C8 phenyl-hexyl cyanopropyl n/a Particle Size μm or 5 μm, spherical μm or 5 μm, spherical μm or 5 μm, spherical μm or 5 μm, spherical μm or 5 μm, spherical Pore Size 00 Å 00 Å 00 Å 00 Å 00 Å Surface Area 00 m/g 00 m/g 00 m/g 00 m/g 00 m/g Carbon Load 0% % 5% 8% n/a End-Cap yes yes yes yes n/a ph Range.5 to to to to to 8.0 Max Temperature 80 C 80 C 80 C 80 C 80 C SIZE (mm) PARTICLE SIZE (µm) ROC C8 ROC C8 ROC PHENYL HEXYL ROC CN ROC SILICA.6 x 50 5 RTK RTK RTK RTK RTK x 50 5 RTK RTK RTK RTK RTK x 00 5 RTK-9555 RTK-9555 RTK RTK RTK x 50 5 RTK-9557E RTK-9557E RTK-95557E RTK-95657E RTK-95057E.0 x 50 5 RTK-9556E RTK-9556E RTK-95556E RTK-95656E RTK-95056E.0 x 00 5 RTK-955E RTK-955E RTK-9555E RTK-9565E RTK-9505E.6 x 50 RTK-9565 RTK-9565 RTK RTK RTK x 00 RTK-955 RTK-955 RTK-9555 RTK-9565 RTK x 50 RTK-956E RTK-956E RTK-9556E RTK-9566E RTK-9506E.0 x 00 RTK-95E RTK-95E RTK-955E RTK-956E RTK-950E Guard Cartridge, /pk RTK RTK RTK RTK RTK Guard Holder RTK-58 RTK-58 RTK-58 RTK-58 RTK-58 Authorized Distributor

81 HPLC COLUMNS Millipore Sigma Millipore Sigma Ascentis Express Outperforms other smaller sub- µm particles in efficiency and addresses other disadvantages inherent in smaller sub- micron UHPLC columns. Delivers perfect balance of pressure and performance ideal for faster small molecule analysis on any UHPLC system. Available in C8, F5, OH5, and HILIC 5i phases. 0. µm. µm FUSED-CORE.0 µm With the addition of.0 µm Ascentis Express UHPLC columns, we now offer three U/HPLC Fused Core particle columns, making the Ascentis Express column line truly scalable from UHPLC to legacy HPLC systems. ASCENTIS EXPRESS SPECIFICATIONS PORE SIZE PRESSURE TEMP LIMIT p H RANGE END- CAPPED 90 Å,500 PSI 60 C 9 Yes Ascentis Express.0 µm Delivers Excellent Peak Shape and Efficiency with Speed Compared to Fully Porous Particle Columns Porous C8,.8 μm 0765 plates peak USP tailing factor peak = PSI Porous C8,.7 μm 687 plates peak USP tailing factor peak =. 0 PSI Ascentis Express C8,.0 μm 786 plates peak USP tailing factor peak =.0 70 PSI Column: Mobile Phase: Flow Rate: Ascentis Express C8 508-U. x 00 mm,.0 µm A: H 0 w/0.% formic acid B: ACN w/0% formic acid, (65:5, A:B) 0.8 ml/min SIZE (mm) C8 F5 OH5 HILIC 5i. x U U 5096-U 58-U. x U 5086-U U 509-U. x U 5086-U U 508-U. x U U U 506-U. x U U 5095-U 50-U. x U U 5095-U 50-U. x 5 Guard Cartridge*, /pk 508-U 5088-U U 50-U.0 x U 5088-U U 59-U.0 x U U U 58-U.0 x U U U 5-U.0 x U 5087-U 5096-U 5-U.0 x U U 5096-U 59-U.0 x 5 Guard Cartridge*, /pk 508-U U 5097-U 5-U Universal EXP Guard Holder, ea 5500-U 5500-U 5500-U 5500-U *Requires Universal EXP Guard Holder Time (min) Temp: 5 C Detection: UV, 0 nm Injection: 0.5 µl Sample: 50 µg/ml in 80:0, water:methanol Instrument: Dionex Ultimate,000. Oxazepam. Nordiazepam. Temazepam. Diazepam Ascentis Express F5 The F5 pentafluorophenylpropyl stationary phase provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C8. Ascentis Express OH5 The OH5 stationary phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage chemistry. The high performance material provides a UHPLC column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) or basic, acidic, zwitterionic, or neutral compounds. Ascentis Express HILIC The Ascentis Express HILIC or bare silica stationary phase provides a UHPLC column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversedphase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds. 8 Chrom Tech your chromatography specialists

82 Millipore Sigma HPLC COLUMNS Millipore Sigma Chromolith RP-8 endcapped High throughput at high flow rates Possibility of flow gradients Improved security, robustness, reliability and versatility Column length no longer pressure limited Cost savings due to increased sample throughput Added performance by column coupling Significantly longer column lifetime Relief from back pressure Cladded Chromolith columns in PEEK are very easy to use and handle ideally suited for use with all standard HPLC instruments Chromolith monolithic HPLC columns combine speed and efficiency like never before. Chromolith column consists of a single rod of high-purity polymeric silica gel with a bimodal pore structure of macro and mesopores. The macropores (average size μm) reduce column back pressure, allowing significantly faster flow rates. The mesopores form a fine porous structure (average pore size nm), which creates a very large active surface area for high-efficiency separations. For even greater efficiency, multiple Chromolith columns can be coupled to achieve a higher theoretical plate count with still very low back pressure. MILLIPORE SIGMA CHROMOLITH RP-8 ENDCAPPED PART NO SIZE (mm) MACROPORE SIZE QTY x 00 µm ea x 00 µm /pk Validation Kit x 50 µm ea x 5 µm ea x 00 µm ea x 00 µm /pk Validation Kit x 50 µm ea x 5 µm ea x 00 µm ea x 50 µm ea x 50 µm /pk Validation Kit x 5 µm ea Chromolith HighResolution Whereas standard Chromolith columns have μm macropores, Chromolith HighResolution columns possess.5 μm macropores. This results in higher efficiency, and improved peak shape. Although this causes higher back pressure, it is still less than half that of any particulate column of the same dimensions. TECH TIP Classic Particulate Columns VS Chromolith HPLC Columns The use of HPLC columns containing the classic or 5 μm small silica particles often results in high back pressure. This may damage both the column and the HPLC system; therefore, classic HPLC columns have limited length and a limited number of theoretical plates. Attempts have been made to increase the plate count by decreasing the particle size, but this results in unacceptable back pressure and limits the variety of separations that can be satisfactorily achieved. Thanks to their revolutionary bimodal pore structure, Chromolith columns provide excellent separations in a fraction of the time that a standard particulate column takes. This enables higher speeds and higher sample throughput at lower back pressure. Validation Kit The Chromolith validation kit includes three columns from three different production batches, allowing you to easily compare batch-to-batch reproducibility and quality. This makes the validation kit an ideal tool for quality control and validation laboratories. MILLIPORE SIGMA CHROMOLITH HIGHRESOLUTION RP-8 ENDCAPPED PART NO SIZE (mm) MACROPORE SIZE QTY x 50.5 µm ea x 00.5 µm ea x 00.5 µm /pk Validation Kit x 50.5 µm ea x 5.5 µm ea Authorized Distributor

83 HPLC COLUMNS Thermo Scientific Accucore Thermo Scientific Accucore HPLC Columns Accucore RP-MS Accucore Phenyl-Hexyl IEX (.7) C AI HR/0 HS SS HBC Optimized for MS detection Excellent combination of speed and quality of separation IEX (.7) C AI HR/0 HS SS HBC Unique selectivity for aromatic and moderately polar analytes BA IEX (7.6) BA IEX (7.6) IEX (.7) AI HR/0 HS Accucore C8 SS Optimum retention for non-polar compounds IEX (.7) AI HR/0 Accucore Phenyl-X HS SS Unique reversedphase shape selectivity with high aromatic selectivity C HBC C HBC BA IEX (7.6) BA IEX (7.6) Accucore C8 Accucore PFP IEX (.7) C AI HR/0 HS SS HBC Lower hydrophobicity than C8 Recommended for analytes with moderate hydrophobicity IEX (.7) C AI HR/0 HS SS HBC Alternate selectivity to C8 particularly for halogenated analytes BA IEX (7.6) BA IEX (7.6) Accucore aq Accucore C0 IEX (.7) AI HR/0 HS SS Compatible with 00% aqueous mobile phases, special selectivity for polar analytes IEX (.7) AI HR/0 HS SS High shape selectivity for hydrophobic, long chain, structurally related isomers C HBC C HBC BA IEX (7.6) BA IEX (7.6) Accucore Polar Premium Accucore HILIC Accucore Urea-HILIC IEX (.7) C AI HR/0 HS SS HBC BA IEX (7.6) Rugged amideembedded C8 phase that offers complementary selectivity to conventional C8 phases Enhanced retention of polar and hydrophilic analytes THERMO SCIENTIFIC UNIGUARD HOLDER Unique HILIC selectivity and low ion exchange activity DESCRIPTION./.0 mm ID.0/.6mm ID Uniguard Drop-In Guard Cartridge Holder Chrom Tech your chromatography specialists

84 Thermo Scientific Accucore HPLC COLUMNS THERMO SCIENTIFIC ACCUCORE COLUMN SPECIFICATIONS PHASES PORE SIZE PARTICLE SIZE ph RANGE CARBON LOAD (%) BACK PRESSURE RATING TEMPERATURE RATING Accucore RP-MS 80 Å.6 µm 9 7,000 bar 70 C Accucore C8 80 Å.6 µm 9,000 bar 70 C Accucore XL C8 80 Å µm bar 70 C Accucore C8 80 Å.6 µm 9 5,000 bar 70 C Accucore XL C8 80 Å µm bar 70 C Accucore aq 80 Å.6 µm 9 9,000 bar 70 C Accucore Phenyl-Hexyl 80 Å.6 µm 8 5,000 bar 70 C Accucore Phenyl-X 80 Å.6 µm 8 6,000 bar 70 C Accucore PFP 80 Å.6 µm 8 5,000 bar 70 C Accucore HILIC 80 Å.6 µm 8,000 bar 70 C Accucore Urea-HILIC 80 Å.6 µm 8,000 bar 70 C SIZE (mm) RP-MS C8 (USP L) XL C8 (USP L) C8 (USP L7) XL C8 (USP L7).6 x aq C8 (USP L).6 x x x x 0 Guard Cartridge*, /pk x x x x x 0 Guard Cartridge*, /pk x x * x * x * x x 0 Guard Cartridge*, /pk SIZE (mm) Polar Premium (USP L60) Phenyl-Hexyl (USP L) Phenyl-X PFP (USP L) C0 (USP L6) HILIC (USP L) Urea-HILIC.6 x x x x 0 Guard Cartridge*, /pk x x x x 0 Guard Cartridge*, /pk x x x x x x 0 Guard Cartridge*, /pk * Requires Uniguard Holder Authorized Distributor

85 HPLC COLUMNS Thermo Scientific Hypersil Gold Thermo Scientific Hypersil Gold Hypersil GOLD Excellent peak shape for all analyte types Hypersil GOLD C8 Similar selectivity to C8 columns, but with reduced retention Hypersil GOLD C Similar selectivity to C8 and C8 columns, but with reduced retention Hypersil GOLD aq Polar endcapped C8 columns, stable in 00% aqueous mobile phase, providing enhanced retention and resolution of polar analytes Hypersil GOLD PFP Perfluorinated phenyl columns offering alternative selectivity. The fluorine atoms around the phenyl ring enhance pi-pi interactions with aromatic molecules Hypersil GOLD Phenyl Excellent retention and unique selectivity for aromatic analytes Hypersil GOLD CN Alternative selectivity with lower hydrophobicity and can also be used for normal phase separations Hypersil GOLD Amino Can be used in reversed phase, normal phase, ion exchange and HILIC modes and are particularly useful for separating carbohydrates Hypersil GOLD SAX A quaternary amine ion exchange ligand ideally for separating small polar organic analytes in aqueous mobile phases Hypersil GOLD Silica Separation of non-polar and moderately polar organic compounds by normal phase chromatography Hypersil GOLD HILIC Enhanced retention of polar and hydrophilic analytes that are problematic using reversed phase columns THERMO SCIENTIFIC UNIGUARD HOLDER DESCRIPTION./.0 mm ID.0/.6 mm ID Uniguard Drop-In Guard Cartridge Holder Chrom Tech your chromatography specialists SIZE (mm) PARTICLE SIZE GOLD C8 C.6 x 50 µm x 00 µm x 50 µm x 0 µm x 50 µm x 00 µm x 50 µm x 50.9 µm x 00.9 µm x 50.9 µm x 00.9 µm x 50.9 µm x 00.9 µm x 50.9 µm x 0.9 µm x 0.9 µm x 00.9 µm x 50.9 µm x 0.9 µm /.6 mm Guard*, /pk µm x 50 µm x 00 µm x 50 µm x 0 µm.0 mm Guard*, /pk µm x 50 µm x 00 µm x 50 µm x 0 µm mm Guard*, /pk µm x 50 µm x 00 µm mm Guard*, /pk µm x 50 5 µm x 50 5 µm x 00 5 µm x 50 5 µm x 0 5 µm x 50 5 µm x 50 5 µm /.6 mm Guard, /pk 5 µm x 50 5 µm x 50 5 µm x 00 5 µm x 50 5 µm x 0 5 µm mm Guard*, /pk 5 µm x 50 5 µm x 50 5 µm x 00 5 µm x 50 5 µm x 0 5 µm mm Guard*, /pk 5 µm * Requires Uniguard Holder

86 Thermo Scientific Hypersil Gold HPLC COLUMNS SIZE (mm) PARTICLE SIZE aq PFP Phenyl CN Amino SAX Silica HILIC.6 x 50 µm x 00 µm x 50 µm x 0 µm x 50 µm x 00 µm x 50 µm x 50.9 µm x 00.9 µm x 50.9 µm x 00.9 µm x 50.9 µm x 00.9 µm x 50.9 µm x 0.9 µm x 0.9 µm x 00.9 µm x 50.9 µm x 0.9 µm.0/.6 mm Guard*, /pk µm x 50 µm x 00 µm x 50 µm x 0 µm mm Guard*, /pk µm x 50 µm x 00 µm x 50 µm x 0 µm mm Guard*, /pk µm x 50 µm x 00 µm mm Guard*, /pk µm x 50 5 µm x 50 5 µm x 00 5 µm x 50 5 µm x 0 5 µm.0 x 50 5 µm x 50 5 µm /.6 mm Guard*, /pk 5 µm x 50 5 µm x 50 5 µm x 00 5 µm x 50 5 µm x 0 5 µm.0 mm Guard*, /pk 5 µm x 50 5 µm x 50 5 µm x 00 5 µm x 50 5 µm x 0 5 µm. mm Guard*, /pk 5 µm * Requires Uniguard Holder Authorized Distributor

87 HPLC COLUMNS Vydac Vydac HPLC/MS Columns Low or NO TFA requirement for sharp symmetrical peptide peaks Retention and selectivity options for a variety of protein and peptide samples ID (µm) LENGTH (mm) C-POLYMERIC C8-POLYMERIC C8-POLYMERIC C8-MONOMERIC MS MS5.55 8MS5.55 8MS MS MS5.55 8MS5.55 8MS MS MS5.50 8MS5.50 8MS MS MS MS MS MS5.5 08MS5.5 8MS5.5 8MS MS5.5 08MS5.5 8MS5.5 8MS MS5.0 08MS5.0 8MS5.0 8MS MS MS5.05 8MS5.05 8MS MS MS5.55 8MS5.55 8MS MS MS5.55 8MS5.55 8MS MS MS5.50 8MS5.50 8MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS MS5 08MS5 8MS5 8MS5 Guard kit ( holder, cartridge) GK5MS/N 08GK5MS/N 8GK5MS/N 8GK5MS/N Repl guard cartridge, /pk GD5MS/N 08GD5MS/N 8GD5MS/N. 50 MS5 08MS5 8MS5 8MS5 Guard kit ( holder, cartridge) 08GK5MS/N 8GK5MS/N 8GK5MS/N Repl guard cartridge, /pk GD5MS/N 08GD5MS/N 8GD5MS/N 8GD5MS/N.6 50 MS5 08MS5 8MS5 8MS5 Guard kit ( holder, cartridge) GK5MS/N 08GK5MS/N 8GK5MS/N 8GK5MS/N Repl guard cartridge, /pk GD5MS/N 08GD5MS/N 8GD5MS/N 8GD5MS/N Vydac 0TP and 0TP 0TP SPECIALTY REVERSED PHASE COLUMN, C8 00Å DESCRIPTION SIZE (mm) PARTICLE SIZE (µm) PART NO Column.6 x TP5 Column.6 x TP55 Column.0 x TP50 Column.0 x TP55 Guard Kit ( holder, cartridge).6 5 0GK5T/N Repl guard cartridge, /pk.6 5 0GD5T/N Guard Kit ( holder, cartridge).6 0 0GK0T/N Repl guard cartridge, /pk.6 0 0GD0T/N Column. x TP5 Column. x TP55 Column.0 x TP5 Column.0 x TP55 Guard Kit ( holder, cartridge). 5 0GK5T/N Repl guard cartridge, /pk. 5 0GD5T/N 0TP HIGH CARBON LOAD REVERSED PHASE COLUMN, C8 00Å DESCRIPTION LENGTH (mm) PARTICLE SIZE (µm) PART NO Column.6 x TP55 Guard holder /N Guard cartridge, /pk.6 5 0GD5T/N Connecter, Guard to Column U-87 0TP columns separate the EPA 6 priority pollutants in less than 0 minutes 0TP Rapid-analysis columns separate the 6 priority pollutant PAHs in under 0 minutes 0TP columns for the analysis of derivatized PAHs U-87 Chrom Tech your chromatography specialists

88 Vydac HPLC COLUMNS Vydac 08TP, TP, 8TP, 9TP, and 8TP 08TP 9TP Peptides up to 0,000-0,000 MW Polypeptides with aromatic side chains Enzymatic digest fragments Large, hydrophobic proteins Natural and synthetic peptides Membrane-spanning peptides Glycoproteins TP Lipid peptides Fusion proteins from inclusion bodies Hemoglobin variants Histones Human growth hormone Insulin variants Membrane proteins 8TP Small polypeptides less than,000-5,000 MW Enzymatic digest fragments Natural and synthetic peptides Complex carbohydrates 8TP Small polypeptides less than,000-5,000 MW Enzymatic digest fragments Natural and synthetic peptides DESCRIPTION SIZE (mm) PARTICLE SIZE ( µm) 08TP C8 00Å TP C 00Å 8TP POLYMERIC C8 00Å 9TP DIPHENYL 00Å 8TP MONOMERIC C8 00Å Column 0 x TP50 TP50 8TP50 9TP50 8TP50 Guard Kit ( holder, cartridge) 0 08FSK0/N FSK0/N 8FSK0/N 9FSK0/N Repl guard cartridge, /pk 0 08GCC0/N GCC0/N 8GCC0/N 9GCC0/N Column.6 x TP5 TP5 8TP5 9TP5 8TP5 ATP5* Column.6 x TP55 TP55 8TP55 9TP55 8TP55 Column.6 x TP505 TP505 8TP505 9TP505 8TP505 Column.6 x 00 08TP0 TP0 8TP0 8TP0 Column.6 x 50 08TP05 TP05 Column.0 x TP5 TP5 8TP5 9TP5 8TP5 Column.0 x TP55 TP55 9TP55 8TP55 Guard Kit ( holder, cartridge) GK0/N GK0/N 8GK0/N 9GK0/N Repl guard cartridge, /pk GD0/N GD0/N 8GD0/N 9GD0/N Guard Kit ( holder, cartridge) GK5/N GK5/N 8GK5/N 9GK5/N 8GK5/N Repl guard cartridge, /pk GD5/N GD5/N 8GD5/N 9GD5/N 8GD5/N Column. x TP5 TP5 8TP5 9TP5 8TP5 Column. x TP55 TP55 8TP55 9TP55 8TP55 Column. x TP505 TP505 8TP505 9TP505 8TP505 Guard Kit ( holder, cartridge). 5 08GK5/N GK5/N 8GK5/N 9GK5/N 8GK5/N Repl guard cartridge, /pk. 5 08GD5/N GD5/N 8GD5/N 9GD5/N 8GD5/N Column.0 x TP5 TP5 8TP5 9TP5 8TP5 Column.0 x TP55 TP55 8TP55 9TP55 8TP55 Column.0 x TP505 TP505 8TP505 9TP505 8TP505 Guard Kit ( holder, cartridge) GK5/N GK5/N 8GK5/N 8GK5/N Repl guard cartridge, /pk GD5/N GD5/N 8GD5/N 8GD5/N *ATP5 is optimized for use in stability analysis of human growth hormone

89 HPLC COLUMNS Zirchrom Zirchrom LC Columns PBD (USP L9) Great peak shapes for basic compounds ZirChrom -PBD is produced by coating ultra-stable zirconia particles with an equally stable extremely thin layer of crosslinked polybutadience. The chemical selectivity is similar to that of a traditional C8 or C8 silica based column for non-ionic analytes. In the case of ionizable analytes there are secondary interactions which can be used to fine tune the chromatographic selectivity (band spacing). The surface chemistry of zirconia is very rich and may be used to change chemical selectivity of the column simply through the addition of mobile phase additives. Consider using ZirChrom -PBD with a phosphate buffer if either the tailing of amines or their selectivities are problematic on C8 silica, and explore the full ph range (ph -) to optimize your separation. For example, a 0 mm phosphate buffer will produce good peak shapes for many ionizable compounds. CARB ZirChrom -CARB is produced by coating a zirconia particle with an extremely thin layer of elemental carbon. The resulting phase gives very different selectivity than any bonded or polymeric phase, and represents an excellent alternative when bonded phases do not provide the required selectivity. ZirChrom -CARB is great for geometric isomer separations, and is superb for separating diastereomers. The selectivity is more different from ODS than phenyl or cyano phases. This makes it an excellent choice for orthogonal screening in drug discovery and impurity profiling. Proper buffer selection helps to ensure the best peak shapes and band spacing. ZirChrom -CARB is stable from ph, and up to 00 C. C8 Ideal for separating steroids and analogues Excellent selectivity for acidic compounds ph stable from for robust methods Excellent thermal stability for fast separations DiamondBond -C8 is made by covalently bonding C8 ligands to the surface of carbon-clad zirconia. This creates the first truly bonded carbon phases in the industry. Because the surface below the C8 ligands is carbon and not silica, DiamondBond -C8 has different selectivity from other phases. DiamondBond -C8 has better peak shapes than unmodified carbon phases, and unique selectivity compared to silica phases. Life for all traditional reversed-phase zirconia products, proper buffer selection helps to ensure the best peak shapes and band spacing. ZirChrom Ion Exchangers Phases for sugars and proteins Wide range of ion exchange selectivity No shrinking or swelling use any organic solvent Significantly higher efficiency than polymeric phases ZirChrom ion exchange phase is produced by coating ultrastable zirconia particles with an extremely thin layer of an ionic polymer. This method creates phases with much higher efficiency and, oftentimes, higher capacity than pure polymeric phases. Also, ZirChrom s ion exchangers do not shrink or swell as a function of ionic strength or organic modifier content of the mobile phase. ZirChrom s SAX phase is thermally stable up to 80 C, which causes different selectivity, allowing high speed separations with lower ionic strength mobile phases. This is very important in the preparation of RNA and DNA samples. If desired, mixed-mode separation modes may be exploited to optimize separations, including Lewis acid-base interactions, hydrophobic interactions and ion-exchange interactions. These modes may be attenuated by adjusting the strong Lewis base content, organic content and ionic strength of the mobile phase, respectively. WAX (Stable from ph 9, and to 80 C) Cross-linked polyethyleneimine-coated zirconia for weak anion-exchange Efficient weak anion-exchanger useful for inorganic and organic anions Useful for the separation of bio-molecules such as nucleotides, nucleosides, oligonucleotides, oligodeoxynucleotides, amino acids, peptides, and proteins Extremely stable amino phase for normal phase separation of carbohydrates. SAX (Stable from ph, and to 80 C) Cross-linked polyethyleneimine-coated zirconia for strong anion-exchange Useful for inorganic and organic anions Ideal for the separation of water-soluble vitamins Useful for the separation of bio-molecules such as nucleotides, nucleosides, oligonucleotides, oligodeoxynucleotides, amino acids, and peptides 6 Chrom Tech your chromatography specialists

90 Zirchrom HPLC COLUMNS Zirchrom LC Columns SIZE (mm) PARTICLE SIZE (µm) PBD CARB Diamond Bond C8 WAX SAX.6 x 50 mm 5 ZR ZR DB ZR ZR x 50 mm 5 ZR ZR DB ZR05-56 ZR x 00 mm 5 ZR ZR DB ZR ZR x 50 mm 5 ZR ZR DB ZR ZR x 50 mm ZR0-56 ZR0-56 DB0-56 ZR ZR x 50 mm ZR0-56 ZR0-56 DB0-56 ZR05-56 ZR x 00 mm ZR0-06 ZR0-06 DB0-06 ZR05-06 ZR x 50 mm ZR0-056 ZR0-056 DB0-056 ZR ZR mm Guard, /pk ZR0-G0 ZR0-G0 DB0-G0 ZR05-G0 ZR06-G0. x 50 mm 5 ZR0-5-5 ZR0-5-5 DB0-5-5 ZR ZR x 50 mm 5 ZR0-5-5 ZR0-5-5 DB0-5-5 ZR ZR x 00 mm 5 ZR0-0-5 ZR0-0-5 DB0-0-5 ZR ZR x 50 mm 5 ZR ZR DB ZR ZR x 50 mm ZR0-5 ZR0-5 DB0-5 ZR05-5 ZR06-5. x 50 mm ZR0-5 ZR0-5 DB0-5 ZR05-5 ZR06-5. x 00 mm ZR0-0 ZR0-0 DB0-0 ZR05-0 ZR06-0. x 50 mm ZR0-05 ZR0-05 DB0-05 ZR05-05 ZR mm Guard, /pk ZR0-G0 ZR0-G0 DB0-G0 ZR05-G0 ZR06-G0 Zirchrom ProTain In-Line Protein Removal System The HPLC analysis of small molecules in matrices containing proteins of variable origin is often problematic because of poor resolution between the analyte of interest and the matrix constituents and potential fouling of the analytical column by matrix proteins and debris. The incorporation of ZirChrom s new ProTain in-line protein removal system upstream of any type of analytical column offers a selective, cost effective and simple method of reducing matrix interferences for the HPLC analysis of small molecules in bio-samples. The type of buffer, specifically its strength as a Lewis base, and the ph of the mobile phase play a significant role in determining the actual protein binding capacity of the ProTain systems. ZirChrom s ProTain in-line protein removal system is comprised of one ProTain system insert and one ProTain system holder. A section of capillary tubing and all of the necessary nuts and ferrules are included with holder. ZIRCHROM PROTAIN IN-LINE PROTEIN REMOVAL SYSTEM SIZE PART NO.6 mm x cm (/pk) PT mm Holder ZR mm x cm (/pk) PT0-0. mm Holder ZR In-Line Removal of Matrix Proteins in HPLC Analysis of Small Molecules Column A: Column B: Mobile Phase: Detector: TSK G000 Size Exclusion Column ZirChrom's in-line ProTain Removal System installed in front of the TSK G000 SEC column 0mM Phosphate buffer at ph 6.8,.0 ml/min 5 nm

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