The Standard for ph Method Development

Transcription

1 The Standard for ph Method Development High Efficiency Excellent Lifetime ph Stable -

2 U.S. Patent No.,, Setting the Standard for ph Method Development Gemini columns are rugged reversed phase HPLC columns that offer extended lifetime at extreme ph conditions and excellent stability for reproducible, high efficiency separations. Take full advantage of high and low ph conditions (ph -) to manipulate selectivity Expect longer column lifetime with patented TWIN-NX technology Phase NX-C C-Phenyl C Description The most rugged Gemini column, offering times the durability of previous generation hybrid columns A low bleed phenyl phase. For UV and MS detection, which offers an aromatic selectivity complementary to C phases Selectivity, high structural integrity and increased loadability for preparative and purification applications in pre-packed columns and bulk media USP Classification L L L For analytical and preparative separations of basic and acidic compounds TWIN (Two-In-ne) Technology Gemini C and C-Phenyl During the final stage of silica manufacturing a unique silica-organic layer is grafted to create a completely new composite particle. nce the internal base silica is unaltered by this manufacturing process, the particle retains its mechanical strength and rigidity along with excellent efficiency, while the silica-organic shell protects the particle from chemical attack. Second-Generation TWIN-NX Technology Gemini NX-C TWIN-NX technology uses an improved patented organo-silica grafting process which incorporates highly stabilizing ethane cross-linking. These organic groups are evenly incorporated into the grafted layers on the silica surface while maintaining a pure silica core. This not only provides resistance to high ph attack, but also maintains the high efficiency and mechanical strength of a silica particle. H H H H H H H H H H H H H H CH CH S CH CH CH CH CH CH CH C CH CH CH CH CH CH CH CH C C CH CH CH CH CH CH CH CH CH 0 Phenomenex, Inc. All rights reserved.

3 How It Works U.S. Patent No.,, Gemini NX-C Ethane Cross-Linking esists High ph Attack ph stable - for durability NX-C Consistent performance in both volatile and non-volatile buffers High sample loading capacity for metabolite identification and preparative purification Gemini NX-C USP: L LC/MS Certified ph Stability:.0.0 Particle ze: µm, µm, and 0 μm Phase: C Application: Small molecules, basic compounds Strength: Most durable ph stable particle Pore ze (Å): 0 Surface Area (m /g): Carbon Load %: End Capping: TMS Multi-Point Ligand Attachment esists Low ph Ligand Cleavage Gemini NX-C Tested for Extreme Durability in Changing Mobile Phase ph Column Efficiencies Maintained in High ph Testing for 0 Cycles 0 00 Vs. Standard lica lica Dissolution % of Initial Efficiencies Stable Efficiencies After Exposure to 0,000 Column Volumes of High and Low ph Buffers Diphenhydramine Cycles of Exposure Tetracaine etention Times of Four Probes Maintained in Neutral ph Testing for 0 Cycles Ligand Cleavage etention in Minutes etention Times Maintained After Exposure to 0,000 Column Volumes of High and Low ph Buffers Sequence Acetophenone Benzene Toluene Acenaphthene Column Used: Column: Gemini NX-C µm Dimensions: 0 x. mm Part No.: 00F--E0

4 NX-C U.S. Patent No.,, Polar Bases at Low ph, ph. Gemini NX-C µm Waters XBridge C µm } } Applications The incredible durability across low and high ph ranges for Gemini NX-C analytical and preparative columns gives scientists the ability to get more performance from their "basic" separations. Polar Bases at High ph, ph 0. Gemini NX-C µm Waters XBridge C µm 0 App ID, 0 App ID min min Polar Bases (Beta Blockers) at High ph Dimensions: 0 x. mm Mobile Phase: A: 0 mm Ammonium Bicarbonate ph 0. B: Acetonitrile Gradient: A/B (:) to (0:0) in min to (0:0) in min, Hold for min Flow ate:. ml/min Detection: 0 nm Sample:. Bisoprolol Contaminant. Sotalol. Atenolol. Labetalol (Diastereoisomeric Pair). Nadolol (Diastereoisomeric Pair). Pindolol. Metoprolol. Bisoprolol. Propranolol 0. Alprenolol App ID 0, App ID 0 0 min 0 0 min Polar Bases (Antihistamines) in Formic Acid Dimensions: 0 x. mm Mobile Phase: A: 0. % Formic Acid in Water B: 0. % Formic Acid in Acetonitrile Gradient: A/B (0:0) to (0:0) in 0 min Flow ate:. ml/min Detection: 0 nm Sample:. Pyrilamine. Tripelennamine. Chlorpheniramine. Brompheniramine. Chloropyramine. Diphenhydramine. Loratadine Comparative chromatograms may not be representative of all applications. XBridge is a trademark and Waters is a registered trademark of Waters Corporation. Phenomenex is in no way affiliated with Waters Corporation.

5 NX-C Applications U.S. Patent No.,, Hydrophobic Basic Drugs at Low ph (ph.) Gemini µmnx -C Waters XBridge µm C App ID 0 App ID 0 0 min 0 0 min Agilent Technologies ZBAX µm Extend-C Thermo Scientific Hypersil GLD µm C App ID App ID 0 0 min 0 0 min Hydrophobic Bases (Diltiazem, Promethazine) in Formic Acid Columns: Gemini µm NX-C XBridge µm C Zorbax µm Extend-C Hypersil Gold µm C Dimensions: 0 x. mm Mobile Phase: A: 0. % Formic Acid in Water B: 0. % Formic Acid in Acetonitrile Gradient: A/B (:) to (:) in 0 min Flow ate:.0 ml/min Detection: nm Sample:. Diltiazem. Promethazine Comparative chromatograms may not be representative of all applications. XBridge is a trademark and Waters is a registered trademark of Waters Corporation. Hypersil GLD is a registered trademark of Thermo Hypersil-Keystone, Inc. Thermo Scientific is a registered trademark of Thermo Fisher Scientific Inc. ZBAX and Agilent Technologies are registered trademarks of Agilent Technologies, Inc. Phenomenex is in no way affiliated with Waters Corp., Thermo Fisher Scientific, or Agilent.

6 NX-C Applications U.S. Patent No.,, Performance in Volatile Buffers With the widespread adoption of LC/MS and LC/MS/MS techniques, column performance in volatile buffers is critical. Gemini µm NX -C Waters XBridge µm C 0 0 App ID App ID 0 0 min 0 0 min Agilent Technologies ZBAX µm Extend-C Thermo Scientific Hypersil GLD µm C 0, 0 App ID App ID min 0 0 min Mixtures of Acids, Neutrals, and Bases in Formic Acid ph. Columns: Gemini µm NX-C XBridge µm C Zorbax µm Extend-C Hypersil Gold µm C Dimensions: 0 x. mm Mobile Phase: A: 0. % Formic Acid in Water B: 0. % Formic Acid in Acetonitrile Gradient: A/B (:) to (0:0) in min, Hold for min Flow ate:. ml/min Detection: nm Sample: Pyridine Quinidine Sulfathiazole Triprolidine Benzyl alcohol Nortriptyline Phenol -Methyl--nitrobenzoic acid Methylsalicylaldehyde Hexanophenone Comparative chromatograms may not be representative of all applications. XBridge is a trademark and Waters is a registered trademark of Waters Corporation. Hypersil GLD is a registered trademark of Thermo Hypersil-Keystone, Inc. Thermo Scientific is a registered trademark of Thermo Fisher Scientific Inc. ZBAX and Agilent Technologies are registered trademarks of Agilent Technologies, Inc. Phenomenex is in no way affiliated with Waters Corp., Thermo Fisher Scientific, or Agilent.

7 NX-C Applications U.S. Patent No.,, Performance in Non-Volatile Buffers Gemini was engineered to be the column of choice for pharmaceutical scientists who work with difficult sample mixtures and harsh mobile phase conditions. The consistent performance and rugged phase will provide simplified method development and long column lifetimes. Gemini µm NX-C Waters XBridge µm C 0 0 App ID App ID 0 0 min 0 0 min Agilent Technologies ZBAX µm Extend-C 0 Thermo Scientific Hypersil GLD µm C 0,, App ID App ID 0 0 min 0 0 min Mixtures of Acids, Neutrals, and Bases in Potassium Phosphate ph. Columns: Gemini µm NX-C XBridge µm C Zorbax µm Extend-C Hypersil Gold µm C Dimensions: 0 x. mm Mobile Phase: A: 0 mm Potassium Phosphate ph. B: Acetonitrile Gradient: A/B (:) to (0:0) in min, Hold for min Flow ate:. ml/min Detection: nm Sample: Pyridine Quinidine Sulfathiazole Triprolidine Benzyl alcohol Phenol Nortriptyline -Methyl--nitrobenzoic acid Methylsalicylaldehyde Hexanophenone Comparative chromatograms may not be representative of all applications. XBridge is a trademark and Waters is a registered trademark of Waters Corporation. Hypersil GLD is a registered trademark of Thermo Hypersil-Keystone, Inc. Thermo Scientific is a registered trademark of Thermo Fisher Scientific Inc. ZBAX and Agilent Technologies are registered trademarks of Agilent Technologies, Inc. Phenomenex is in no way affiliated with Waters Corp., Thermo Fisher Scientific, or Agilent.

8 C U.S. Patent No.,, Increased loading and retention of basic compounds lica efficiency and mechanical strength ph stable - for durability Chromatographic Comparisons Gemini C USP: L LC/MS Certified ph Stability:.0.0 Particle ze: µm, µm, and 0 μm Phase: C Application: Small molecules, basic compounds Strength: Wide ph stability, high efficiency Pore ze (Å): 0 Surface Area (m /g): Carbon Load %: End Capping: TMS Gemini µm C 0 Å Agilent Technologies Zorbax µm Extend-C 0 Å Virtually no silanol interactions! 0 0 min Tricyclic Antidepressants at Neutral ph Dimensions: 0 x. mm Mobile Phase: 0 mm Phosphate buffer ph.0/acetonitrile/ Methanol (0::) Flow ate:. ml/min Detection: nm Sample:. Nortriptyline. Imipramine. Amitriptyline. Clomipramine Batch-to-Batch eproducibility App ID 0 0 min 0 0 min Advanced Chromatography Technologies ACE C µm 00 Å Extended Column Lifetime App ID 0 App ID The Twin Technology engineering of Gemini provides stability and increased column lifetime. Whether used under isocratic or gradient conditions, Gemini out-performs and outlasts ph stable columns. This is illustrated below. App ID Lifetime and Efficiency Comparison. times increased column lifetime and still going! Batch Batch Batch Batch 0 min Efficiency (N) Columns fail after 0% loss in efficiency. Gemini Fail Line XTerra Fail Line Conditions for all separations: Column: Gemini C μm Dimension: 0 x. mm Part No.: 00F--E0 Mobile Phase: 0 mm Ammonium Bicarbonate, ph 0./ Acetonitrile (0:0) Flow ate:.0 ml/min Detection: 0 nm Sample:. Pindolol. Metoprolol. Propranolol Days Efficiency and lifetime comparison based on average of two columns each run in parallel. Columns: Gemini C μm Waters XTerra MS C μm Dimensions: 0 x. mm Mobile Phase: Acetonitrile/0 mm Methylpyrrolidine Buffer, ph. (0:0) Flow ate: ml/min Detection: nm Sample: Diphenhydramine Gemini C Waters XTerra MS C Comparative chromatograms may not be representative of all applications. XTerra and Waters are registered trademarks of Waters Corporation. ZBAX and Agilent Technologies are registered trademarks of Agilent Technologies, Inc. ACE is a registered trademark of Advanced Chromatography Technologies (ACT). Phenomenex is in no way affiliated with Waters Corp.,ACT, or Agilient.

9 C-Phenyl U.S. Patent No.,, ph stable - for durability Great aromatic selectivity Extremely low UV and MS bleed Gemini C-Phenyl USP: L LC/MS Certified ph Stability:.0.0 Particle ze: µm and µm Phase: Phenyl with C linker Application: Aromatic, polar or basic compounds Strength: High aromatic selectivity with exceptional peak shape even in neutral conditions. Extremely low bleed phenyl column. Pore ze (Å): 0 Surface Area (m /g): Carbon Load %: End Capping: TMS Enhanced Performance for Aromatic Compounds Sulfa Drug Application Pursuit DiPhenyl µm Gemini C-Phenyl µm esolution s,.0.0 s,..0 Dimensions: 0 x. mm Mobile Phase: 0. % Formic Acid in Water/ Methanol (0:0) Flow ate:.0 ml/min Detection: nm Sample:. Sulfathiazole. Sulfamerazine. Sulfamethoxazole mau 0 mau mau 0 mau Phenomenex Gemini C-Phenyl μm App ID 0 min min Agilent Technologies Pursuit μm Di Phenyl App ID 0 min min eproducible Phenyl Phase Aliphatic Acid Application Low Bleed Phenyl Phase Intensity cps.0e.0e Popular C.0e.0e.0e.0e Gemini C-Phenyl.0e.0e.0e Blank 0 min min Column: Gemini C-Phenyl µm Dimensions: 0 x. mm Part No.: 00F--E0 Mobile Phase: 0 mm Phosphate buffer, ph./methanol (:) Flow ate:.0 ml/min Detection: 0 nm Sample:. Tartaric Acid. Acetic Acid. Malic Acid. Citric Acid. Lactic Acid. Propionic Acid Dimensions: 0 x.0 mm Mobile Phase: A: 0. % Formic acid in Water B: 0. % Formic acid in Acetonitrile Gradient: % B to % B in 0 min, then hold % B for min Flow ate: 0. ml/min MS Detection: ESI + ion mode, M/Z Comparative chromatograms may not be representative of all applications. Pursuit and Agilent Technologies are registered trademarks of Agilent Technologies, Inc. Phenomenex is in no way affiliated with Agilent.

10 0 If Gemini analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, send in your comparative data within days and keep the Gemini column for FEE.

11 rdering Information μm Microbore, Minibore and Narrow Bore Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 0 x.0 0 x.0 0 x.0 00 x.0 0 x.0 0 x.0 00 x.0 0 x.0 x.0* /0pk C 00B--A0 00M--B0 00A--B0 00B--B0 00D--B0 00F--B0 00B--Y0 00D--Y0 00F--Y0 AJ0- C-Phenyl 00B--A0 00A--B0 00B--B0 00D--B0 00F--B0 00B--Y0 00D--Y0 00F--Y0 AJ0- /0pk NX-C 00M--B0 00A--B0 00B--B0 00D--B0 00F--B0 00B--Y0 00D--Y0 00F--Y0 AJ0- for ID:.0-.0 mm μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 0 x. 0 x. 00 x. 0 x. 0 x. x.0* /0pk C 00M--D0 00A--E0 00B--E0 00D--E0 00F--E0 00G--E0 AJ0- C-Phenyl 00A--E0 00B--E0 00D--E0 00F--E0 00G--E0 AJ0- /0pk NX-C 00B--E0 00D--E0 00F--E0 00G--E0 AJ0- for ID:.-.0 mm μm Minibore and Narrow Bore Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 0 x.0 0 x.0 0 x.0 0 x.0 00 x.0 0 x.0 0 x.0 x.0* /0pk C 00A--B0 00B--B0 00F--B0 00G--B0 00B--Y0 00D--Y0 00F--Y0 00G--Y0 AJ0- C-Phenyl 00A--B0 00B--B0 00F--B0 00B--Y0 00F--Y0 00G--Y0 AJ0- /0pk NX-C 00A--B0 00B--B0 00F--B0 00B--Y0 00D--Y0 00F--Y0 00G--Y0 AJ0- μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x. 0 x. 00 x. 0 x. 0 x. x.0* /0pk C 00A--E0 00B--E0 00D--E0 00F--E0 00G--E0 AJ0- C-Phenyl 00A--E0 00B--E0 00D--E0 00F--E0 00G--E0 AJ0- /0pk NX-C 00B--E0 00D--E0 00F--E0 00G--E0 AJ0- for ID:.-.0 mm μm Semi-Prep Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x 0 0 x 0 0 x 0 /pk C 00F--N0 00G--N0 AJ0- C-Phenyl 00G--N0 AJ0- /pk NX-C 00F--N0 00G--N0 AJ0- for ID: - mm Axia Packed Preparative Columns (mm) for ID:.0-.0 mm For Gemini Capillary HPLC Columns, Guards, and Adapter, contact your Phenomenex technical consultant or local distributor. SecurityGuard Cartridges (mm) Phases 0 x. 00 x. 0 x. 0 x. 0 x 0 x 0 x.** x 0.0 μm /ea /ea C 00B--P0-AX 00D--P0-AX 00F--P0-AX 00G--P0-AX 00B--U0-AX 00C--U0-AX AJ0- AJ0-0 C-Phenyl 00D--P0-AX 00F--P0-AX 00G--P0-AX 00C--U0-AX AJ0- AJ0- μm /ea /ea NX-C 00B--P0-AX 00D--P0-AX 00F--P0-AX 00G--P0-AX 00B--U0-AX 00C--U0-AX AJ0-0 AJ0-0 μm /ea /ea C 00B--P0-AX 00D--P0-AX 00F--P0-AX 00G--P0-AX 00B--U0-AX AJ0- AJ0-0 0 μm /ea /ea NX-C 00B--P0-AX 00D--P0-AX 00F--P0-AX 00G--P0-AX AJ0-0 AJ0- for ID: - mm 0- mm Axia Packed Preparative Columns (mm) continued SecurityGuard Cartridges (mm) Phases 00 x 0 0 x 0 0 x 0 0 x 0 00 x 0 0 x 0 0 x 0 x 0.0 μm Inquire Inquire Inquire /ea C 00D--U0-AX 00F--U0-AX 00G--U0-AX 00B--V0-AX AJ0-0 C-Phenyl 00D--U0-AX AJ0- μm Inquire Inquire /ea NX-C 00D--U0-AX 00F--U0-AX 00G--U0-AX AJ0-0 μm Inquire Inquire Inquire Inquire Inquire Inquire /ea C 00D--U0-AX 00F--U0-AX 00G--U0-AX 00B--V0-AX 00D--V0-AX 00F--V0-AX 00G--V0-AX AJ0-0 0 μm Inquire Inquire Inquire Inquire Inquire Inquire /ea NX-C 00D--U0-AX 00F--U0-AX 00G--U0-AX 00B--V0-AX 00D--V0-AX 00F--V0-AX 00G--V0-AX AJ0- for ID: 0- mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ0- SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-0 **PEP SecurityGuard Cartridges require holder, Part No.: AJ0- PEP SecurityGuard Cartridges require holder, Part No.: AJ0-

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