ProntoSIL HPLC Columns

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ProntoSIL HPLC Columns For Technical Assistance Call -800--708 analytical inc.

2 ProntoSIL HPLC Columns For Technical Assistance Call analytical inc. To Order and For More Information: Call FAX We ship nearly 00% of your orders the same day they are placed. Delivery is normally by UPS ground. At your option, orders received by pm ET can be delivered overnight, second day or third day select (second day service is standard on shipments west of the Mississippi). Web te:

Contents ProntoSIL HPLC Columns Page ProntoSIL C8-EPS Reversed-Phase HPLC Columns................................... Part Numbers and Prices for ProntoSIL C8-EPS Reversed-Phase HPLC Columns.

................................................ Part Numbers and Prices for ProntoPEARL sub HPLC Columns..................... 7 Additional ProntoSIL HPLC Columns.

3 Contents ProntoSIL HPLC Columns Page ProntoSIL C8-EPS Reversed-Phase HPLC Columns Part Numbers and Prices for ProntoSIL C8-EPS Reversed-Phase HPLC Columns ProntoSIL C0 Reversed-Phase HPLC Columns Part Numbers and Prices for ProntoSIL C0 Reversed-Phase HPLC Columns ProntoPEARL sub HPLC Columns Part Numbers and Prices for ProntoPEARL sub HPLC Columns Additional ProntoSIL HPLC Columns Part Numbers and Prices for Additional ProntoSIL HPLC Columns ProntoSIL 00Å Wide Pore HPLC Columns Part Numbers and Prices for ProntoSIL 00Å Wide Pore HPLC Columns

ProntoSIL C8-EPS Reversed-Phase HPLC Columns Provides excellent separation of polar compounds Better peak shape for acids and bases Stabilized bonded phase for rugged, robust HPLC methods More

4 ProntoSIL C8-EPS Reversed-Phase HPLC Columns Provides excellent separation of polar compounds Better peak shape for acids and bases Stabilized bonded phase for rugged, robust HPLC methods More retentive than ordinary polar embedded phases Enhanced Polar Selectivity for High Resolution Separations of Polar Compounds A high performance base deactivated column with enhanced polar selectivity specifically designed for high resolution separation of polar compounds. Provides Excellent Separation of Polar Compounds Base deactivated stationary phases generally provide better peak shape, increased column efficiency, and improved lot-to-lot reproducibility when separating polar compounds. They have been particularly useful in improving the separation of samples containing basic compounds. However, there are occasions when typical base deactivated phases lack adequate selectivity. FIGURE Enhanced Polar Selectivity and Excellent Peak Shape for Basic Compounds Stationary phases that permit polar-polar interaction through silanol activity offer alternate selectivity to base deactivated phases, but it often comes at a cost of poor peak shape and uncertain reproducibility. ProntoSIL C8-EPS solves this problem by providing both enhanced polar selectivity and excellent peak shape (Figure ). Enhanced Polar Selectivity The ProntoSIL C8-EPS has an amide group strategically placed in the bonded phase (Figure ). This polar amide group adds polar characteristics to this very hydrophobic stationary phase (Figure ). With this enhanced polar selectivity, ProntoSIL C8-EPS provides a powerful alternate selectivity to typical base deactivated phases. FIGURE ProntoSIL C8-EPS Bonded Phase Column: ProntoSIL C8-EPS,. x 0 mm Mobile Phase: % CH OH, % Phosphate buffer, ph 7.0 Sample:. Uracil. Imipramine. Protriptyline. Amitriptyline 7 8. Nortriptyline. Doxepine 7. Trimipramine 8. Clomipramine lica Support O O CH Amide C8 Phase The ProntoSIL C8-EPS uses an amide group to add polar selectivity to this highly hydrophobic stationary phase. ProntoSIL C8-EPS is specifically designed for high resolution separations of polar compounds. A unique polar embedded group adds polar selectivity to this highly retentive phase and also shields the silica surface so that excellent peak shape for basic compounds can be achieved. Specifications Phase: C8 with amide embedded group Particle ze: and µm Pore ze: 0Å Surface Area: 00 m/g Carbon Load: 8% ph Range: - 0 Manufacturers of base deactivated columns try to minimize polar-polar interactions between analytes and the stationary phase. Because of this, most base deactivated columns have similar selectivity for polar compounds. This means that if one brand of base deactivated column lacks selectivity to adequately separate a pair of polar solutes, other brands of base deactivated columns will also probably lack adequate selectivity for these solutes. Better Peak Shape for Acids and Bases ProntoSIL C8-EPS has an amide group strategically placed close to the surface of the silica support. This not only permits the amide group to provide polar characteristics to the stationary phase, it also shields the silica surface and inhibits polar interactions between solutes and silanols. This shielding allows for exceptionally good peak shape for bases. Unlike some other polar embedded phases, ProntoSIL C8-EPS also provides excellent peak shape for acids.

5 ProntoSIL C8-EPS Reversed-Phase HPLC Columns FIGURE Enhanced Polar Selectivity Typical Base Deactivated C8 α = 0.89 Summary ProntoSIL C8-EPS columns with enhanced polar selectivity give you a powerful advantage when developing separations of polar compounds. Not only will this column provide you with excellent peak shape for both acids and bases, its stabilized bonded phase will allow you to develop rugged, robust HPLC methods over a broad ph range to 0! Best of all, ProntoSIL columns are sold and supported by MAC-MOD Analytical, the people you have come to trust when it comes to HPLC columns. 0 ProntoSIL C8-EPS α =. FIGURE Dual Bonding Resists Loss of Bonded Phase ProntoSIL C8-EPS with Dual loxane Bonds lica Support O Polar C8 Phase O Polar C8 Phase O CH O CH 0 8 Dual bonds resist acid hydrolysis Bonded phase unaffected Mobile Phase: % CH CN % Phosphate buffer, ph. Sample:. p-butylbenzoic acid. N,N-Dimethylaniline The ProntoSIL C8-EPS column with enhanced polar selectivity provides significantly better selectivity for this pair of polar solutes than a typical base deactivated column. Stabilized Bonded Phase for Rugged, Robust HPLC Methods A major cause of column failure is the loss of bonded phase from the silica support due to hydrolysis of the siloxane bond. ProntoSIL C8-EPS uses a unique, proprietary bidentate bonding chemistry that provides a dual bond to the silica surface that stubbornly resists loss of bonded phase (Figure ). This stabilized bonded phase greatly extends the lifetime of ProntoSIL C8-EPS columns. The proprietary bonding chemistry also inhibits dissolution of the silica. The result is an unusually stable column that can confidently be used over a ph range much wider than other reversed phase columns, to 0! More Retention than Ordinary Polar Embedded Phases Other polar embedded phases use either less hydrophobic C or C phases, or have part of their alkyl phase shielded by their polar embedded group. ProntoSIL C8-EPS is a true C8 phase with an octadecyl phase placed after the polar amide group. As a result, ProntoSIL C8-EPS provides more retention than other polar embedded phases (Figure ). Typical Bonded Phase lica Support CH O C8 Phase CH Acid hydrolysis cleaves siloxane bond C8 Phase Acid hydrolysis of the siloxane bond and the resulting loss of bonded phase (i.e., C8 Phase) is one of the major reasons for column failure. ProntoSIL C8-EPS uses bidentate bonding chemistry to secure the polar C8 phase to the silica support through a dual siloxane bond. The dual bonding inhibits the loss of bonded phase and makes these columns unusually stable, even under conditions that quickly kill other columns. FIGURE Comparison of Retention Symmetry Shield C8 7 7 ProntoSIL C8-EPS Zorbax Bonus RP Column:. x 0 mm Mobile Phase: 0% CH CN 0% H O Flow Rate:.0 ml/min Sample:. Formaldehyde. Propanale. Acetaldehyde. Crotonaldehyde. Acetone 7. Butylraldehyde. Acroleine ProntoSIL C8-EPS is more hydrophobic than other polar embedded phases and, therefore, provides more retention. Discovery Amide 7 CH O CH Bonded phase lost ProntoSIL C8-EPS Ordering Information Dimensions Particle (mm) ze (µm) Part Number Price.0 x 0 00F8APS00 $ 8.0 x 0 00F8APS x 7 070F8APS00.0 x 7 070F8APS00.0 x 00 00F8APS00.0 x 00 00F8APS x 0 0F8APS x 0 0F8APS x 0 0F8APS00 9. x 0 0F8APS00 8. x 0 0F8APS00 8. x 7 07F8APS00. x 7 07F8APS00. x 00 0F8APS00. x 00 0F8APS x 0 F8APS00 9. x 0 F8APS00 0. x 0 F8APS x 0 80F8APS x 0 0F8APS00, Analytical Guard Cartridges (Guard Catridge Holder required).0 x 0 (pk) F8APS x 0 (pk) 0F8APS00 7 Guard Cartridge Holder Semi-Preparative and Preparative Guard Columns 8.0 x 0 080F8APS00G 8.0 x F8APS00G 0.0 x 00F8APS00G x 0 00F8APS00G 0

ProntoSIL C0 Reversed-Phase HPLC Columns Unique planar selectivity for carotenoids and structural isomers Excellent reproducibility Multi-valent silane bonding for enhanced stability FIGURE

of Hydrophobic Structural Isomers ProntoSIL C0 HPLC columns are particularly recommended for the separation of hydrophobic, long-chain, structural isomers.

susceptible to column fouling. ProntoSIL C0 columns show significantly greater shape selectivity compared to C8 phases due to their rigid, highly ordered C0 alkyl groups (Figure ).

FIGURE 7 HPLC Separation of Carotenoids all-trans 9-cis For best performance, C0 columns should be used at ambient temperature or lower.

6 ProntoSIL C0 Reversed-Phase HPLC Columns Unique planar selectivity for carotenoids and structural isomers Excellent reproducibility Multi-valent silane bonding for enhanced stability FIGURE Comparison of C0 and C8 Phases for the Separation of Lutein Stereoisomers C8 Mobile Phase: 7% Acetone % H O,, C0 Mobile Phase: 8% Acetone % H O Reversed Phase HPLC Columns for the Separation of Hydrophobic Structural Isomers ProntoSIL C0 HPLC columns are particularly recommended for the separation of hydrophobic, long-chain, structural isomers. They are often a better alternative to normal phase columns for the separation of isomers since they are not as sensitive as normal phase columns to water content of the mobile phase and are not as susceptible to column fouling. ProntoSIL C0 columns show significantly greater shape selectivity compared to C8 phases due to their rigid, highly ordered C0 alkyl groups (Figure ). This shape selectivity advantage makes them the ideal HPLC column to use for the separation of carotenoids (Figure 7) and tocopherol derivatives (Figure 8). FIGURE 7 HPLC Separation of Carotenoids all-trans 9-cis For best performance, C0 columns should be used at ambient temperature or lower. At elevated temperatures, the C0 alkyl chains will become less ordered and lose their shape selectivity (Figure 9). In addition, C0 phases should be used with mobile phases containing at least 0% organic modifier to avoid phase collapse. Although you can expect lower plate count on C0 phases compared to C8 phases, the ability of the C0 phases to separate isomers that are not possible to separate on C8 phases make them an attractive choice for many applications. C0 phases have been successfully used for the analysis of plant extracts, food, and biological tissues that contain structurally different carotenoids and mixtures of geometric isomers. They are also commonly used for separating stereoisomers of vitamin E and vitamin A, and for the separation of PAHs. FIGURE 8 HPLC Separation of Tocopherol Isomers Column: ProntoSIL C0,. x 0 mm Mobile Phase: 9% Methanol, % Water Flow Rate: 0.9 ml/min Temperature: C Sample: ) δ-tocopherol ) γ-tocopherol ) β-tocopherol ) α-tocopherol Flow Rate: ml/min Detector: UV at 0 nm Sample: ) -Z lutein, ) -Z lutein, ) all-e-lutein ) 9-Z lutein, ) 9 -Z lutein Column: ProntoSIL C0,. x 0 mm Mobile Phase: 80% Methanol, 0% TBME Flow Rate:. ml/min Temperature: 0 C By courtesy of Prof. Dr. K. Albert et al, University Tuebingen, Germany.

7 ProntoSIL C0 Reversed-Phase HPLC Columns FIGURE 9 The Effect of Temperature on Shape Selectivity of C0 Phases Column: ProntoSIL C0 0 C FIGURE 0 HPLC Separation of Steroids (Metabolites of Norethisterone Acetate) 0 C C C -cis all-trans 9-cis Mobile Phase: Flow Rate: Detection: Sample: 0% to 00% B, 0 minutes A: Water B: Methanol 0. ml/min UV at 0 nm ) -Keto-Norethisterone acetate ) Estradiol ) Norethisterone acetate By courtesy of Prof. Dr. K. Albert et al, University Tuebingen, Germany. ProntoSIL C0 Ordering Information Dimensions Particle (mm) ze (µm) Part Number Price.0 x 0 00H00PS00 $ 8.0 x 0 00H00PS x 7 070H00PS00.0 x 7 070H00PS00.0 x 00 00H00PS00.0 x 00 00H00PS00.0 x 0 0H00PS x 0 0H00PS x 0 0H00PS00. x 0 0H00PS00 8. x 0 0H00PS00 8. x 7 07H00PS00. x 7 07H00PS00. x 00 0H00PS00. x 00 0H00PS00. x 0 H00PS x 0 H00PS00 9. x 0 H00PS00. x 0 H00PS x 0 80H00PS x 0 0H00PS00, Guard Cartridges (Guard Cartridge Holder required).0 x 0 ( pk) 0H00PS x 0 ( pk) H00PS00 7 Guard Cartridge Holder Specifications Stationary Phase Support: Spherical, ultra-pure, Type B silica and micron particle size 00 Angstrom pore size 00 m /gram surface area Bonded Phase: Polymeric bonding chemistry, non-endcapped C0 (Triacontylsilane) 8.% carbon load

8 ProntoPEARL sub HPLC Columns Two particle types -.8 µm totally porous (TPP) for high-speed separations of small molecules -. µm non-porous (NPP) for high-speed separation of proteins Multiple bonded phases - TPP available in C8-EPS, C8-EPS, aminopropyl - NPP available in C8 Optimized hardware design -, 0,, 0, 0, 7 and 00 mm lengths -.0,.0,. mm internal diameter FIGURE Separation of Polyphenols A New Generation of Columns for Increased Productivity ProntoPEARL columns are engineered for Ultra Fast Chromatography. Columns of totally porous particles (TPP) are available in lengths from mm to 00 mm. Non-porous columns (NPP) come in lengths of mm to 0mm. Due to the extraordinary efficiency of these.8 µm and. µm particles (00,000 plates per meter guaranteed), it is possible to drastically reduce analysis times without negatively impacting resolution. Reduce Analysis Time 90% Using Standard HPLC Equipment High back pressure can be a major deterrent to using micro-particle columns. The process used to manufacture ProntoPEARL columns, however, assures a narrow particle size distribution, eliminating fines from the packed column bed. A 0 mm ProntoPEARL column can be operated in a conventional HPLC system at flow rates up to.0 ml/min. nce a column of this size is equivalent to a conventional 0 mm µm column, you can easily achieve a 90% reduction in analysis time within the pressure and flow limits of your current liquid chromatograph. 80 nm 70 nm FIGURE Fast Protein Separation Column: ProntoPEARL TPP sub C8-EPS,. x 0 mm Mobile Phase: 0% Acetonitrile 70% 0 mm Phosphate buffer, ph Flow Rate:.0 ml/min 00 Temperature: 70 C Detection: UV 80 nm and UV 70 nm 00 Sample:. Myricetine,. cis-reveratrol,. trans-resveratrol,. Quercitine,. Caempferol Absorbance [mau] % ACN 0 TABLE ProntoPEARL sub Specifications Pore Particle Surface Carbon Phase ze (Å) ze (µm) area(m /g) Loading (%) Pronto PEARL sub TPP C8-EPS Pronto PEARL sub TPP C8-EPS Pronto PEARL sub TPP APS Pronto PEARL sub NPP C8 NA Column: ProntoPEARL NPP sub C8,. x mm Mobile Phase: A: 0% ACN, 80% H O + 0.% TFA B: 0% ACN, 0% H O + 0.% TFA Gradient: 0-0% in 0. min 0-00% in 0.7 min Flow Rate: ml/min Detection: UV nm Sample:. Ribonuclease A. Cytochrome C. Lysozyme. α-lactalbumin. Albumin, Bovine. Myoglobin 7. β-lactoglobulin B 8. β-lactoglobulin A 0

9 ProntoSIL PEARL sub HPLC Columns FIGURE The Performance Test Column: ProntoSIL C8 EPS Dimension: 0 x. mm, µm Flow:.0 ml/min 8 Column: ProntoPEARL TPP sub C8 EPS Dimension: 0 x. mm Flow:. ml/min 9 Reach Lower Detection Limits Because of the high efficiency and lower dispersion rates of ProntoPEARL columns, it is now possible to achieve higher mass sensitivity and therefore lower detection limits than formerly obtained using conventional particles. To realize the full gain in both efficiency and sensitivity, extra column volume in the instrument should be minimized. It is recommended that tubing from the sample injector through the detector be replaced with precision cut 0.00 stainless steel. A data acquisition rate of 0 Hz is also required Mobile Phase: 0% Acetonitrile 0% Water Detection: nm Temperature: 0 C Sample:. Uracil.Toluene. Phenol 7. Naphthalene. Acetophenone 8. Acenaphthene. Benzoic acid ethyl ester 9. Anthracene. N,N Dimethylaniline ProntoPEARL sub TPP Ordering Information Dimensions Particle Price (mm) ze (µm) C8 - EPS C8 - EPS NH (USD).0 x.8 00C8ATP08 00C08ATP08 00C90TP08 $.0 x.8 00C8ATP08 00C08ATP08 00C90TP08. x.8 0C8ATP08 0C08ATP08 0C90TP08.0 x 0.8 A0C8ATP08 A0C08ATP08 A0C90TP x 0.8 A0C8ATP08 A0C08ATP08 A0C90TP08 8. x 0.8 AC8ATP08 AC08ATP08 AC90TP x.8 00C8ATP08 00C08ATP08 00C90TP x.8 00C8ATP08 00C08ATP08 00C90TP08 8. x.8 0C8ATP08 0C08ATP08 0C90TP x C8ATP08 00C08ATP08 00C90TP08.0 x C8ATP08 00C08ATP08 00C90TP08. x 0.8 0C8ATP08 0C08ATP08 0C90TP08.0 x C8ATP08 00C08ATP08 00C90TP x C8ATP08 00C08ATP08 00C90TP08 8. x 0.8 0C8ATP08 0C08ATP08 0C90TP x C8ATP08 070C08ATP08 070C90TP08.0 x C8ATP08 070C08ATP08 070C90TP08. x C8ATP08 07C08ATP08 07C90TP08.0 x C8ATP08 00C08ATP08 00C90TP x C8ATP08 00C08ATP08 00C90TP08 7. x C8ATP08 0C08ATP08 0C90TP08 7 ProntoPEARL sub NPP Ordering Information Dimensions Particle Price (mm) ze (µm) C8 (USD).0 x. 00Z89NP0 $.0 x. 00Z89NP0.0 x 0. A0Z89NP x 0. A0Z89NP0 77. x 0. AZ89NP x. 00Z89NP0 9.0 x. 00Z89NP0 9. x. 0Z89NP0 9.0 x 0. 00Z89NP x 0. 00Z89NP0 70. x 0. 0Z89NP x 0. 00Z89NP0 7.0 x 0. 00Z89NP0 7. x 0. 0Z89NP0 7 7

Additional High Performance ProntoSIL HPLC Columns ProntoSIL C8 SH A highly retentive base-deactivated C8 phase that provides excellent peak shape for basic compounds.

10 Additional High Performance ProntoSIL HPLC Columns ProntoSIL C8 SH A highly retentive base-deactivated C8 phase that provides excellent peak shape for basic compounds. The ProntoSIL C8 SH has a stabilized bonded phase that provides rugged, reliable, long-term performance, even at mobile phase ph as low as.0. ProntoSIL C8 SH A base-deactivated phase that uses the same stabilized bonding technology as the ProntoSIL C8 SH. The ProntoSIL C8 SH also provides excellent peak shape for basic compounds, but is less hydrophobic than the ProntoSIL C8 SH. ProntoSIL Phenyl The ProntoSIL Phenyl offers an alternate selectivity to the C8 and C8 phases. It also has a stabilized bonded phase that provides long-term reliable performance. ProntoSIL CN A cyano-propyl bonded phase that can be used in either normal phase or reversedphase mode. ProntoSIL C8 AQ A special C8 phase that is recommended for the separation of polar compounds that require high aqueous mobile phase conditions. Hydrophilic endcapping keeps the C8 chains fully extended into the mobile phase, even when using aqueous mobile phases with no organic modifier. The result is better retention and reproducibility than can be achieved with typical C8 phases when using mobile phases with less than 0% organic modifier. Additional ProntoSIL Ordering Information Dimensions Particle Price (mm) ze (µm) C8 SH C8 SH Phenyl CN C8 AQ (USD).0 x 0 00F80PS00 00F080PS00 00F00PS00 00F00PS00 00F8PS00 $ 8.0 x 0 00F80PS00 00F080PS00 00F00PS00 00F00PS00 00F8PS x 7 070F80PS00 070F080PS00 070F00PS00 070F00PS00 070F8PS00.0 x 7 070F80PS00 070F080PS00 070F00PS00 070F00PS00 070F8PS00.0 x 00 00F80PS00 00F080PS00 00F00PS00 00F00PS00 00F8PS00.0 x 00 00F80PS00 00F080PS00 00F00PS00 00F00PS00 00F8PS x 0 0F80PS00 0F080PS00 0F00PS00 0F00PS00 0F8PS x 0 0F80PS00 0F080PS00 0F00PS00 0F00PS00 0F8PS x 0 0F80PS00 0F080PS00 0F00PS00 0F00PS00 0F8PS00 9. x 0 0F80PS00 0F080PS00 0F00PS00 0F00PS00 0F8PS00 8. x 0 0F80PS00 0F080PS00 0F00PS00 0F00PS00 0F8PS00 8. x 7 07F80PS00 07F080PS00 07F00PS00 07F00PS00 07F8PS00. x 7 07F80PS00 07F080PS00 07F00PS00 07F00PS00 07F8PS00. x 00 0F80PS00 0F080PS00 0F00PS00 0F00PS00 0F8PS00. x 00 0F80PS00 0F080PS00 0F00PS00 0F00PS00 0F8PS x 0 F80PS00 F080PS00 F00PS00 F00PS00 F8PS00 9. x 0 F80PS00 F080PS00 F00PS00 F00PS00 F8PS00 0. x 0 F80PS00 F080PS00 F00PS00 F00PS00 F8PS x 0 80F80PS00 80F080PS00 80F00PS00 80F00PS00 80F8PS x 0 0F80PS00 0F080PS00 0F00PS00 0F00PS00 0F8PS00 Guard Cartridges (Guard Cartridge Holder required).0 x 0 ( pk) F80PS00 F080PS00 F00PS00 F00PS00 F8PS x 0 ( pk) 0F80PS00 0F080PS00 0F00PS00 0F00PS00 0F8PS00 7 Guard Cartridge Holder

11 ProntoSIL 00Å Wide Pore These wide pore phases are recommended for the separation of proteins and peptides. As with all ProntoSIL phases, ultra pure silica is used for the stationary phase support, and the latest bonding technology is used to provide rugged, reliable separations. C8, C8, and C bonded phase are available. FIGURE Separation of Tryptic Digest of Casein ProntoSIL C8 00H Additional ProntoSIL HPLC Columns Column:. x 7 mm,. µm Mobile Phase: Gradient: 0-0% B, min A: Water with 0.% TFA B: 70% Acetonitrile 0% Water with 0.% TFA Flow Rate:.0 ml/min Detector: UV, 0nm Temperature: 0 C Sample: Tryptic digest of casein FIGURE Separation of Peptides on ProntoSIL C8 00H ProntoSIL C8 00H High efficiency ProntoSIL C8 00H micron material packed in a short column provides high speed, high resolution separation of tryptic digests. Specifications for ProntoSIL 00Å Phases Particle Pore Surface Carbon Phase End capped ze (µm) ze Å Area (m /g) Load (%) C8 00 H yes, C8 00 SH yes, Column:. x 0 mm, µm Mobile Phase: Gradient: 0-00% B, 0 min A: Water with 0.% TFA B: 70% Acetonitrile 0% Water with 0.% TFA Flow Rate:. ml/min Detector: UV, 0nm Temperature: 0 C Sample:. Oxytocin. Eledoisi. Bradykinin. Neurotensin. Angiotensin II. Angiotensin I The wide pore ProntoSIL C8 00H column is recommended for the separation of peptides. C 00 yes, ProntoSIL 00Å Wide Pore Ordering Information Dimensions Particle Price (mm) ze (µm) C8 00 H C8 00 SH C 00 (USD).0 x 0 00K8PS00 00K080PS00 00K00PS00 $ 8.0 x 0 00K8PS00 00K080PS00 00K00PS x 7 070K8PS00 070K080PS00 070K00PS00.0 x 7 070K8PS00 070K080PS00 070K00PS00.0 x 00 00K8PS00 00K080PS00 00K00PS00.0 x 00 00K8PS00 00K080PS00 00K00PS00.0 x 0 0K8PS00 0K080PS00 0K00PS x 0 0K8PS00 0K080PS00 0K00PS x 0 0K8PS00 0K080PS00 0K00PS00. x 0 0K8PS00 0K080PS00 0K00PS00 8. x 0 0K8PS00 0K080PS00 0K00PS00 8. x 7 07K8PS00 07K080PS00 07K00PS00. x 7 07K8PS00 07K080PS00 07K00PS00. x 00 0K8PS00 0K080PS00 0K00PS00. x 00 0K8PS00 0K080PS00 0K00PS00. x 0 K8PS00 K080PS00 K00PS x 0 K8PS00 K080PS00 K00PS00 9. x 0 K8PS00 K080PS00 K00PS x 0 80K8PS00 80K080PS00 80K00PS x 0 0K8PS00 0K080PS00 0K00PS00, Guard Cartridges (Guard Cartridge Holder required).0 x 0 K8PS00 K080PS00 K00PS x 0 0K8PS00 0K080PS00 0K00PS00 7 Guard Cartridge Holder

Demand Flawlessness. HPLC Columns GERMAN ENGINEERED PERFORMANCE lica Support O O CH Amide C8 Phase Reusable End Fitting Typical Base-Deactivated C8 α = 0.

Refillable Column Engineered for Better Performance and Longer Column Life. ProntoSIL HPLC columns are designed to provide higher performance and longer column life.

12 Demand Flawlessness. HPLC Columns GERMAN ENGINEERED PERFORMANCE lica Support O O CH Amide C8 Phase Reusable End Fitting Typical Base-Deactivated C8 α = 0.89 ProntoSIL C8-EPS is a polar embedded phase with superior stability due to its dual siloxane bonding. Universal Fitting Adapter Multi-layered Filter Element 0 ProntoSIL C8-EPS α =. Refillable Column Engineered for Better Performance and Longer Column Life. ProntoSIL HPLC columns are designed to provide higher performance and longer column life. An innovative multi-layered filter element, rather than the common stainless steel frit used by most manufacturers, provides a more efficient transport of sample to the top of the stationary phase. The result is superior chromatography and a dramatically reduced occurrence of column plugging. 0 8 Mobile Phase: % CH CN % Phosphate buffer, ph. Sample:. p-butylbenzoic acid. N, N-Dimethylaniline ProntoSIL C8-EPS provides enhanced polar selectivity and improved peak shape compared to typical base deactivated phases. Engineered to be Excellent. Choose ProntoSIL for German engineering at its best. Demand Flawlessness. Demand ProntoSIL. TO ORDER ProntoSIL OR FOR MORE INFORMATION visit or call analytical inc. MAC-MOD Analytical, Inc. P.O. Box 00 / 0 Commons Court / Chadds Ford, PA 97 / phone: / fax: / info@mac-mod.com PRONTOSIL IS MANUFACTURED BY BISCHOFF CHROMATOGRAPHY, LEONBERG, GERMANY LC 7 Effective 8/0

ProntoSIL C18-EPS Reversed-Phase HPLC Columns

ProntoSIL C18-EPS Reversed-Phase HPLC Columns ProntoSIL C8-EPS Reversed-Phase HPLC Columns Provides excellent separation of polar compounds Better peak shape for acids and bases Stabilized bonded phase for rugged, robust HPLC methods More retentive