KromasilSFC. Designed for green technology. Introducing Kromasil SFC XT

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1 KromasilSFC Designed for green technology Introducing Kromasil SFC XT

2 Basedon00Åporesize,.andµmparticles,KromasilSFC columns give users the opportunity of fast separations. The columns are tailor-made for research, discovery and routine analysis. Many options Kromasil SFC columns are the answer for fast separations. These columns are now being delivered in cyano, diol, silica, -ethylpyridine and fused organo-silane chemistries for the laboratory scientist to separate a wide range of substances, from non-polar to strongly polar compounds. The stationary phase quintet SIL CN [min] [min] Separation of β-blockers Byusingthisstandardsetof Kromasil SFC columns, the user can efficiently screen the material thatworksbestforagivensample. Diol EP XT New! [min] [min] [min] Stationary phase: Kromasil SFC,. μm phase chemistry as in figure Columnsize:.0x0mm Part numbers: FHSIC, FHCNC, FHDIC, FHEPC and FHXTC Eluent:CO /methanol+0mmammonia Gradient:0min:%,0min:0%methanol Flow rate:.0 ml/min Outlet pressure: 0 bar Detection:UV@0nm Substances: = alprenolol =propranolol =acebutolol =pindolol Chromatograms for SIL, CN, Diol and EP in this figure are a courtesy of AstraZeneca, Mölndal, Sweden

3 Product characteristics Kromasil SFC is based on first-in-class, perfectly spherical, porous Kromasil silica particles with00åporesizeandparticlesizesof.andµm.furthermore,kromasilsfcxtisthe result of a patented organic/inorganic merged silica technology. SIL: Bare silica Diol: Diol Coverage:.µmol/m XT: Fused organo-silane CN: Cyano Coverage:.0µmol/m EP: -ethylpyridine Coverage:.8µmol/m Fast separations Medium and high-throughput laboratories working with green technology and seeking toimproveturnaroundtimearenowableto take advantage of the separation power of the newkromasilsfc.µmfamilyofcolumns. With the chromatographic power of Kromasil SFC phases available in short and narrow columns, users can easily achieve baseline resolution and ultra-fast separations. Separation of steroids [min] Column:KromasilSFC,.μm,EP,.0x0mm Part number: FHEPC0 Eluent:CO /methanol Gradient:0min:0%,min:0%methanol Flow rate:. ml/min Outlet pressure: 0 bar Detection:UV@0nm Substances: = deoxycorticosterone = corticosterone =cortisone = hydrocortisone

4 In general, the EP functionality is known for its benefits in terms of selectivity and retentioninsfc,andithasthespecific benefits towards the separation of basic compounds, where peak shape is significantly improved. Therefore the EP chemistry is seen as the workhorse for SFC. With the introduction of Kromasil SFC XT we cannowofferasfcphasethatgreatly compliments EP showing orthogonality towards EP and other common SFC phases when run under standard SFC conditions. This new material provides additional tools to SFC users around the world. Elution order for common acidic, neutral and basic substances on selected SFC phases. Relative elution time increases downwards in the figure. elution time Kromasil SFC Diol anthracene acetophenone flavanon pyridine ibuprofen caffeine benzoic acid phenol salicylic acid acetylsalisylic acid fenoprofen flurbiprofen benzocaine naproxen deoxycorticosterone ketoprofen carbamazepine phenytoin corticosterone cortisone primidone prednisone pyrogallol hydrocortisone prednisolone lidocain phloroglucinol alprenolol amitriptyline propranolol acebutolol pindolol Kromasil SFC XT acetophenone anthracene flavanon ibuprofen benzoic acid pyridine salicylic acid phenol fenoprofen flurbiprofen acetylsalisylic acid naproxen benzocaine ketoprofen caffeine deoxycorticosterone phenytoin lidocain pyrogallol carbamazepine corticosterone cortisone prednisone primidone phloroglucinol hydrocortisone prednisolone alprenolol propranolol amitriptyline acebutolol pindolol Kromasil SFC EP pyridine acetophenone lidocain flavanon caffeine anthracene amitriptyline deoxycorticosterone carbamazepine benzocaine phenol ibuprofen benzoic acid alprenolol primidone corticosterone phenytoin fenoprofen acetylsalisylic acid flurbiprofen naproxen cortisone ketoprofen prednisone propranolol hydrocortisone prednisolone acebutolol pindolol pyrogallol salicylic acid phloroglucinol Stationary phase: Kromasil SFC,. μm, phase chemistries as in table Columnsize:.0x0mm Part numbers: FHDIC, FHXTC and FHEPC Eluent:CO /methanol Gradient:0min:%,min:0%methanol Flow rate:. ml/min Outlet pressure: 0 bar Detection:UV@nm

5 Alternative selectivity The new Kromasil SFC XT offers additional interactions with the stationary phase surface, creating alternative selectivities. SIL XT Separation of anilines on KromasilSFCSILandXT 0 [min] 0 [min] 6 Columns: Kromasil SFC,. μm, SIL andxt,.0x0mm Part numbers: FHSIC, FHXTC Eluent:CO /methanol Gradient:0min:%,7min:9%methanol Substances: = aniline =ethylaniline = -nitroaniline = -nitroaniline = -nitroaniline Flow rate: ml/min Outlet pressure: 0 bar Detection:UV@0nm Application Separation of local anesthetics SIL CN EP 0 [min] 6 0 [min] 0 [min] Stationary phase: Kromasil SFC,. μm, phase chemistry as in figure Columnsize:.0x0mm Part numbers: FHSIC, FHCNC and FHEPC Eluent: CO /methanol+0mmammonia Gradient: 0 min: %, min: 0% methanol Flow rate:.0 ml/min Outlet pressure: 0 bar Detection: UV@ 0 nm Substances: = lidocaine =benzocaine =bupivacaine =tetracaine =procaine

6 AllKromasilSFCphasesareavailableas.andμmparticlessizes withcolumnsupto0mmi.d.inthiswaywecanofferproductsthat give users the possibility to seamlessly transition between different particle and column sizes and easily scale up their separations. Similarly to LC, almost all preparative separation starts at analytical scale using either smaller particles, more narrow columnsoracombinationofthetwo.bydevelopingthe preparative method on an analytical scale, SFC users are able to minimize solvent and sample usage while shorten time for method development. Maintained selectivity Whether in analytical or preparative scale,. or μm particles, Kromasil offers the same material produced in a similar way with similar specifications. This means users can be sure that the separation itself will stay identical during scale up. Kromasil SFC-.-XT.0 x 00 mm Kromasil SFC--XT 0 x 0 mm ɑ =.6 ɑ =.6 0 [min] [min] 0 Part number: FHXTC0 Eluent:CO /0%methanol Flow rate:. ml/min Substance: = phenol, = phenytoin, = hydrocortisone Outlet pressure: 0 bar Detection:UV@nm Part number: F0XTP Eluent:CO /0%methanol Flow rate: 8. ml/min Substances: = phenol, = phenytoin, = hydrocortisone Outlet pressure: 00 bar Detection:UV@nm 6

7 Purification of prednisolone Because of the high surface availability of our SFC line, our materials show great loadability which makes them excellent choices for preparative purification under overloaded conditions. Analytical injection of crude Preparative injection fraction collection 0. [min] 0 8 [min] Analytical conditions Column: Kromasil SFC-.-XT.0 x 00 mm Part number: FHXTC0 Eluent: CO /methanol Gradient:0min:0%,min:0%methanol Flow rate:.0 ml/min Outlet pressure: 0 bar Detection:UV@0nm Preparative conditions Column: Kromasil SFC--XT 0 x 0 mm Part number: F0XTP Eluent: CO /0%methanol Loading: 6 mg Flow rate: 8.0 ml/min Outlet pressure: 00 bar Detection:UV@nm Application Separation of hydrobenzenes SIL Diol XT 0 6 [min] [min] [min] 9 Stationary phase: Kromasil SFC,. μm, phase chemistry as in figure Columnsize:.0x0mm Part numbers: FHSIC, FHDIC and FHXTC Eluent:CO / methanol Gradient:0min:%,0min:%methanol Flow rate:.0 ml/min Outlet pressure: 0 bar Detection:UV@0nm Substances: = benzyl alcohol =resorcinol =catechol =hydroquinone = phloroglucinol 7

8 Availability The Kromasil SFC columns are available in sizes from.0 to 0 mm i.d. They are packed with stationary phases with. and µm particle sizes and the following surface chemistries: silica (SIL), cyano (CN), diol (Diol), -ethylpyridine (EP) and fused organo-silane surface (XT). The moment you adopt our Kromasil High Performance Concept, you join thousands of chromatographers who share a common goal: to achieve better separations when analyzing or isolating pharmaceuticals or other substances. PUBBsfcx_80 Akzo Nobel Pulp and Performance Chemicals AB, 08 Kromasil is a registered trademark of AkzoNobel Specialty Chemicals in a number of territories in the world. Not only will you benefit from our patented silica technology, but you gain a strong partner with a reliable track record in the field of silica products. For the past 70 years, we have pioneered new types of silica. Our long experience in the field of silica chemistry is the secret behind the development of Kromasil, and the success of our Separation Products group. Kromasil is available in bulk and in high-pressure slurry-packed columns. The development, production and marketing of Kromasil are ISO 900 certified. Kromasil is a brand of AkzoNobel Specialty Chemicals, a worldwide suplier for manufacturers of everyday products such as paper, plastics, building materials, and personal care items. Building on the dedication of our employees and our shared commitment to safety, sustainability, and open innovation, we have established a world-class business and built strong partnerships with our customers. via neera 8/a 0 Milano - tel fax cps@cps.it