Rotator PTR-35. Operating instructions

Transcription

1 Rtatr PTR-35

2 Versin January 2012 Page 2

3 Cntents 1 Safety General Infrmatin Getting Started Operatin f PTR Specificatins Guarantee and service...13 Versin January 2012 Page 3

4 1. Safety The fllwing symbl means: Cautin! Read these perating instructins fully befre use and pay particular attentin t sectins cntaining this symbl. GENERAL SAFETY Use nly as specified in the perating instructins prvided. The unit shuld be saved frm shcks r drps. The unit must be stred and transprted in a hrizntal psitin (see package label). After transprt r strage allw the unit t dry ut (2-3 hrs) befre cnnecting t the supply vltage. Befre using any cleaning r decntaminatin methd except thse recmmended by the manufacturer, check with the manufacturer that the prpsed methd will nt damage the equipment. D nt make mdificatins t the design f the unit. As the unit is prducing shaking r rtatinal mvement, be aware f the surface that the unit will be placed n. ELECTRICAL SAFETY Cnnect nly t a pwer supply with a vltage crrespnding t that n the serial number label. Use nly the external pwer supply unit prvided with this prduct. Ensure that the external pwer supply cnnectr is easily accessible during use. Befre mving the unit, discnnect it frm the mains. T turn ff the unit, discnnect the external pwer supply frm the pwer utlet. If liquid is spilt inside the unit, discnnect it frm the external pwer supply and have it checked by a cmpetent persn. DURING OPERATION D nt perate the unit in envirnments with aggressive r explsive chemical mixtures. D nt perate the unit if it is faulty r been incrrectly installed. Fr indr use nly. D nt use utside labratry rms. D nt place a lad exceeding maximum lading mentined in p. Specificatins. BIOLOGICAL SAFETY It is the user's respnsibility t carry ut apprpriate decntaminatin if hazardus material is spilt n r inside the equipment. Versin January 2012 Page 4

5 2. General Infrmatin prvides: Orbital rtatinal mtin, Reciprcal mtin, Vibrating mtin f the platfrm in different planes. The micrprcessr cntrl allws t set a prgram fr realisatin f nt nly separate mixing mtins but als fr the cnsecutive realisatin f different mtin types n the cyclical principle. The setting ptins are: Applicatins Speed and time f rdinary ROTATIONAL MOTION (360º) f the platfrm fr a time perid sec, r nn-stp rpm. Segment f RECIPROCAL MOTION when the directin f platfrm rtatinal mtin frm the vertical plane is changing in turns within the limits f the set segment (turning angle 1-90º fr a time perid sec, r nn-stp); Segment and time f VIBRO MOTION f the platfrm (turning angle 1-5º fr a time perid 1-5 sec) run n the brders f reciprcal mtin segment. It is available nly when the reciprcal mtin is ON; PAUSE duratin fr temprary platfrm mtin stps (1-5 sec) when the vibr mtin is ff (the turning angle f vibr mtin is set t zer) run n the brders f reciprcal mtin segment. It is available nly when the reciprcal mtin is ON; Wrking perid frm 1 min t 24 hurs, r nn-stp. is designed fr mixing bilgical slutins, cell suspensins, magnetic particles cnjugated with specific antibdies as well as incubatin and cultivatin f bilgical liquids accrding t the peratr set prgram. The device is applicable in all areas f labratry research in bitechnlgy, micrbilgy, chemistry, and medicine. Versin January 2012 Page 5

6 3. Getting started 3.1 Unpacking Remve packing materials carefully, and retain fr future shipment r strage f the unit. 3.2 set includes:...1 piece Platfrm PRS piece External pwer supply...1 piece ; Certificate...1 cpy Optinal accessries: platfrm PRS n request platfrm PRS-10...n request platfrm PRSC-22...n request platfrm PRSC-10...n request 3.3 Set up: Place the unit n the hrizntal even wrking surface. Plug the external pwer supply unit int the 12 V scket at the rear side f the unit. 3.4 Platfrm replacement: Screw ut tw fixing screws n the platfrm. Replace the platfrm and install the new platfrm securing it with the screws. Fix the screw tightly. 3.5 Principle peratin The principle f peratin f the PTR-35 is based n the creatin f rtatinal mvement f the platfrm in the vertical plane prviding effective mixing f bilgical liquids in tubes r micrplates. The cntrl keys n the frnt panel prvide prgram setting and peratin cntrl. Versin January 2012 Page 6

7 3.6 The three types f mtin The PTR-35 prvides 3 types f mtin, which can be used separately (except fr vibratin mde, which wrks in cnjunctin with reciprcal mtin mde) and cnsecutively in a cycle: Rtating mtin (Orbital) Simple even circular mtin - cmmn type f mtin used in Rtatrs. Adjustable speed frm 1 t 100 RPM. Reciprcating Rtating mtin (Reciprcal) Vertical rtatin with changing directin f rtatin. Adjustable turning angle (frm 1 t 90, increments f 1 ) sets the limits fr this type f mtin. The speed is the same as set fr rtatinal mtin frm the vertical plane (frm 1 t 100 RPM). In this type f mtin there is a pause functin (frm 1 t 5 secnds,in crement f 1 secnd), this can be set in the Vibratin/pause mde. Vibratin mtin (Vibr) Intensive mixing f samples at high speed with small amplitude requires a Vibrating mtin. The vibratin mde decreases adhesin f liquids t the tube surface, this is especially imprtant when using micr quantities when the sample weight may be equal t the surface adhesin frce. The chice f vibratin mde depends n a number f parameters, namely viscsity, surface adhesin, vlume and specific gravity f the bi-sample. Versin January 2012 Page 7

8 4. Operatin f PTR-35 Multi-Rtatr Orbital (rpm) Reciprcal (deg.) Vibr/ /pause (sec.) (hr:min) RUN Select Run Stp Recmmendatin during peratin When lading use even number f tubes arranged symmetrically t the rtatin axis t give the unit even balance during peratin. 4.1 Cnnect the external pwer supply t the mains. 4.2 Place samples n the platfrm: Eppendrf tubes up t the end; vacutainers and tubes with caps - halfsize. 4.3 Set the apprpriate prgram and peratin time accrding t the methdical prescriptins (see 4.11). 4.4 Press Run/Stp key (u) t start the prgram. 4.5 The platfrm mtin begins and the crrespnding indicatin [RUN] (v) and the changing time values are displayed. 4.6 If the peratin time is nt set and the timer indicatr (w) shws [0:00], pressing Run\Stp key causes cntinuus peratin f the unit until the Run\Stp key is pressed again. 4.7 If the peratin time is set then the unit stps after the set time interval has elapsed, (flashing indicatin STOP n the display) and a sund signals the end f the peratin (press Run\Stp key t stp the signal). Versin January 2012 Page 8

9 4.8 Fr repeat peratin f the previus prgram press Run\Stp key. 4.9 If necessary the PTR-35 can be stpped at any time during peratin by pressing Run/Stp key. In this case platfrm mtin stps when the platfrm achieves hrizntal psitin. Pressing Run/Stp key again will start the prgram frm the beginning (cuntdwn timer will be restarted). Nte! A stepper mtr is used in this mdel. This allws the user t stp the platfrm with their hand fr a mment - withut causing damage t the unit. If the platfrm is stpped by hand during the peratin, the prgram is nt interrupted and the platfrm mtin is autmatically resumed after the platfrm is released Once yu have finished using the unit, unplug the external pwer supply frm the mains utlet t turn ff the unit Prgram setting Press Select key ( x) t chse the parameter t change (the active parameter is flashing). Use and keys ( y) t set the necessary value (nte: if the key is pressed fr mre that 2 secnds the display changes quickly) The prgram can als be changed during the peratin - the micrprcessr autmatically enters the last changes int the memry during the prgram peratin The cuntdwn timer is used t cntrl the peratin time. The timer can be set fr a perid frm 1 minute t 24 hurs (timer increment f 1 minute) The examples belw shw separate mtin types and their available cmbinatins in cycles. Nte! When setting prgram parameters fr peratin with higher lads n PTR-35 please mind that the unit may nt perfrm at highest settings in reciprcal and vibratin mdes. The recmmended lad is indicated in table 1 n page Mtin cycles Orbital Rtatin Set the speed f Orbital rtatin (u1 t 100 RPM), time f Orbital rtatin (v1 t 250 secnds) and time fr Reciprcal mtin t [OFF] (w) Orbital + Reciprcal Rtatin Set the speed (u1 t 100 RPM) and time (v 1 t 250 secnds) f Orbital rtatin. Set the turning angle (w1 t 90º) and time (x1 t 250 secnds) fr Reciprcal mtin. Switch ff the Vibrating mtin (yset the time f Vibrating mtin t [0]) ff Orbital + Reciprcal + Vibratin Set the speed (u1 t 100 RPM) and time (v 1 t 250 secnds) f Orbital rtatin. Set the angle (w1 t 90º) and time (x1 t 250 secnds) fr Reciprcal mtin. Set the turning angle (y1 t 5º) and time (z1 t 5 secnds) fr Vibrating mtin. Orbital (rpm) Reciprcal (deg.) Vibr/ /pause ¹ (hr:min) : OFF 2 STOP Orbital (rpm) 200 Reciprcal (deg.) 125 V ibr/ /pause ¹ (hr:min) :00 Orbital (rpm) Reciprcal (deg.) 0 STOP V ibr/ /pause ¹ (hr:min) : STOP Versin January 2012 Page 9

10 Nte! If the set time f Reciprcal mtin is shrter r equal t the set time f Vibrating mtin then the Reciprcal mtin will be mitted (Orbital + Vibratin) Orbital + Reciprcal + Pause Set the speed (u1 t 100 RPM) and time (v1 t 250 secnds) f Orbital rtatin. Set the turning angle (w1 t 90º) and time (x1 t 250 secnds) fr Reciprcal mtin. Set the angle f Vibrating mtin t [0](y). Set the time fr Vibratin/pause mde (z1 t 5 secnds) - this is the time f pause duratin. Orbital (rpm) Reciprcal (deg.) Vibr/ /pause (hr:min) : STOP ¹ Nte! If the set time f Reciprcal mtin shrter r equal t the set time f Vibratin/pause mde, the Reciprcal mtin will be mitted (Orbital + Pause) Reciprcal Rtatin Set time fr Orbital rtatin t zer (u) [OFF]. Set the turning angle (v1 t 90º) and time (w 1 t 250 secnds) f Reciprcal mtin. Set the time fr Vibrating mtin t [OFF] (x). Orbital (rpm) Reciprcal (deg.) V ibr/ /pause (hr:min) :00 OFF 125 OFF STOP ¹ Reciprcal + Pause Set time f Orbital rtatin t zer (u) [OFF]. Set the turning angle (v1 t 90º) and time (w 1 t 250 secnds) f Reciprcal mtin. Set the time fr Vibrating mtin type (y1 t 5 secnds) - this is the time f pause duratin. Set the angle f Vibrating mtin t [0] (x) Vibratin + Reciprcal Rtatin Set time f Orbital rtatin t zer (u) [OFF]. Set the turning angle (v1 t 90º) and time (w 1 t 250 secnds) f Reciprcal mtin. Set the angle (x) (1 t 5º) and time (1 t 5 secnds) f Vibrating mtin. Orbital (rpm) Reciprcal (deg.) V ibr/ /pause ¹ (hr:min) :00 OFF STOP Orbital (rpm) Reciprcal (deg.) V ibr/ /pause ¹ (hr:min) :00 OFF STOP Nte! Nrmally PTR-35 perfrms sft vibratin. Hwever there is a mde fr hard vibratin. T perfrm hard vibratin set the turning angle f Reciprcal mtin (u) t 90º and the angle f Vibrating mtin (v) t 1º (Hard Vibratin). Orbital Reciprcal V ibr/ (rpm) (deg.) /pause (hr:min) ¹ :00 OFF STOP Wrking in vibr mtin mde fr lng perid nn-stp and using the platfrm with universal rubber clamps, chse the tubes nt lnger than 7 cm frm cap till bttm. Versin January 2012 Page 10

11 5. Specificatins Operating cnditins The unit is designed fr peratin in cld rms, incubatrs and clsed labratry rms at ambient temperature frm +4 C t +40 C and maximum relative humidity 80% fr temperatures up t 31 C decreasing linearly t 50% relative humidity at 40 C. Rtating mtin Speed range rpm r secnds Vertical rtatin mvement...360º Reciprcal mtin Turning angle...1 t 90 (increment 1 ) r...0 t 250 secnds Vibratin mtin / pause Turning angle...1 t 5 (increment 1 ) r...1 t 5 sec Pause...1 t 5 sec General specificatins Prgram timer...1 t 24 hurs (increment 1 min) r nn-stp Dimensins (W x D x H) x 195 x 155 mm Input current/pwer cnsumptin...12v, 660 ma / 8 W External pwer supply... input AC V 50/60Hz, utput DC 12V Weight, nt mre than...1,8 kg Grant is cmmitted t a cntinuus prgramme f imprvement, specificatins may be changed withut ntice. Versin January 2012 Page 11

12 * PRS series platfrms are equipped with universal rubber clamps fr different size tube fixatin; PRSC series platfrms have metal clamps able t hld heavier slutins (e.g.sil,sand). Mtin type Recmmended lad Tubes Weight Rtatin Full lad Up t 450 g. Reciprcal rtatin 26 Eppendrf type tubes; 8-15 ml Falcn tubes, 2-50 ml Falcn tubes Up t 100 g. fr platfrm with rubber clamps; r 200 g. fr platfrm with metal clumps Vibr mtin Eppendrf type tubes nly Up t 100 g. Table 1. Recmmended lading fr PTR-35 depending n mtin type Versin January 2012 Page 12

13 6. Guarantee and Service 6.1 Guarantee 6.2 Service When used in labratry cnditins and accrding t these wrking instructins, this prduct is guaranteed fr TWO YEARS against faulty materials r wrkmanship. Fr service, return fr repair t ur Service Department in the UK r, in ther cuntries, t ur distributr. Versin January 2012 Page 13

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16 Grant Instruments (Cambridge) Ltd Shepreth, Cambridgeshire SG8 6GB UK Tel: +44 (0) Fax: +44 (0) Rtatr/PTR-35/29974/3.01