Nanostructured sensors. Raphaël Pugin Section Head Nanoscale Technology
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1 Nanostructured sensors Raphaël Pugin Section Head Nanoscale Technology
2 Why nanostructures? Adhesion & wettability Optics Photovoltaics Sensors CSEM s DID Superhydrophobicity Anti-icing Dry-adhesion Friction Cell-substrates interactions Anti-reflective properties Structural colours Plasmonic 1µm Light trapping structures Higher sensitivity Faster responsiveness Lower drift Copyright 2017 CSEM Raphaël Pugin Page 1
3 CSEM objective and strategy Develop processes and manufacturing chains for the large volume fabrication of submicro-/nano- structured surfaces, films, components with enhanced performances or unique properties. Origination of Micro-/Nanostructures and Upscaling or Replication Surface functionalization Characterization & Integration Self- Assembly eg. Nanosphere lithography Sol-gel processes Dry etching Hot embossing UV nanoimprint Injection Molding Slot Die Coating Molecular vapor deposition (SAMs, metal oxide thin films) Dye or Enzyme Encapsultation Performance evaluation Demo: Nanostructured functional components Copyright 2017 CSEM Raphaël Pugin Page 2
4 Outline Manufacturing of plastic nanostructured biodiagnostic platform Origination of low-cost, large scale nanostructures Upscaling or replication of nanostructures by injection molding Demonstration of improved performances (signal homogeneity, sensitivity) Manufacturing of disposable patch & film for gas sensing Fabrication of functional mesoporous sol-gel film Integration for Life Sciences Pressure Sensitive Painting for Aeronautics Air quality monitoring application Copyright 2017 CSEM Raphaël Pugin Page 3
5 Nanostructured biodiagnostic platform Objectives: Improve sensitivity of injection-molding biodiagnostic platform with nanostructuration Control of the wettability of detection spots Improve signal quality and homogeneity Tooling: fabrication of nanostructures on a mold insert presenting microchannels and micropins Replication : optimization of the replication process for nanostructured micromolds Copyright 2017 CSEM Raphaël Pugin Page 4
6 Micro- and nano-structures tested Origination of nanostructures by beads and polymer selfassembly Hot embossed S0 Hot embossed S1 Replication into plastic, fabrication of nanostructured biodiagnostic platform: Hot embossing and Injection molding: PC parts with four different structures Hot embossed S2 Hot embossed S3 Characterization: Influence of structuring on wettability Biodiagnostic platform : fluorescence immunoassay Injection molded micro Injection molded nano Copyright 2017 CSEM Raphaël Pugin Page 5
![Water contact angle [ ] Influence of structuring on wettability Dynamic](/docs-images/86/94636105/images/7-0.jpg "contact angles of water measured on the four types of hot embossed")
7 Water contact angle [ ] Influence of structuring on wettability Dynamic contact angles of water measured on the four types of hot embossed structures and, as a control, flat PC PC FLAT PC S0 PC S1 S16 PC S2 S28 PC S3 S29 bicontinuous Advancing water contact angle and contact angle hysteresis significantly increased Wetting mode : Wenzel type (sticky drops) Copyright 2017 CSEM Raphaël Pugin Page 6
8 Normalised gain Tests using a model immunoassay In these tests, the antibody used for detection is inkjet-printed on the spots of the bio-diagnostic platform. The fluorescent spots were imaged using a confocal microscope Flat S1 S2 S3 Results: Better spot homogeneity: no coffee-ring effect after spotting 30% increase in fluorescence for structure S3 (increase in specific surface, different roughness, possible scattering of the emitted light) Copyright 2017 CSEM Raphaël Pugin Page 7
9 Manufacturing of functional mesoporous films Objective: Develop disposable sensitive film enabling the optical detection of dissolved analytes or gases Low cost manufacturing process Higher performance: shorter response time, higher sensitivity Manufacturing: Optimize and upscale sol-gel coating Highly controlled range of porosity and chemical composition Copyright 2017 CSEM Raphaël Pugin Page 8
10 New chemically sensitive optical patches State-of-the-art: sensitive molecules embedded into a matrix (polymeric, inorganic) Encapsulation of active species into a secondary microporous matrix Mesoporous Hierarchically porous Advantages: Dye chemical protection Reduction of self-quenching Larger interface, larger loading rate, higher emitted intensity Open structure, negligible impact on response time Enhanced sensitivity to O 2 /CO 2 Lower impact of H 2 O Linear sensitivity curves Enhanced lifetime No drift CSEM Patent pending Copyright 2017 CSEM Raphaël Pugin Page 9
11 Demox Determine oygen concentration in cell and tissue culture Bio-compatible low cost sensor (disposable SG patch) Customizable readers equipped with integrated optics and electronics CSEM provides complete solution Bio-Innovation Prize awarded to CSEM by the Fondation Eclosion Copyright 2017 CSEM Raphaël Pugin Page 10
12 O2 sensor performances Frequency-domain lifetime fluorimetry SPECIFICATIONS accuracy and confidence interval (CI) Single point calibration in ambient air 0-21% O 2 (stable environment) O 2 sensing in gas phase at 23/37 C and 70/90% humidity Accuracy 0.1% at 2% O 2 0.2% at 21% O 2 Precision - 95% confidence interval ±0.3% at 2% O 2 ±0.3% at 21% O 2 Reference fluorimeter Copyright 2017 CSEM Raphaël Pugin Page 11
13 Sensitive mesoporous layers for aeronautic Pressure-Sensitive Paintings (PSP) for aero-dynamic testing in wind tunnel: mesoporous sol-gel coatings are used to measure fast pressure variations observed in an unsteady aerodynamic flow Higher sensibility when compared to standard PSP, better performances in luminescence (10x) and responsiveness (F acq. up to 10KHz vs F acq. < 1KHz for std PSP). Technology patented, will be transfered to ONERA ( ) for commercialization CCIFS Innovation Trophy awarded to CSEM Copyright 2017 CSEM Raphaël Pugin Page 12
14 CO 2 sensor for air quality monitoring, prototype fabricated Highly sensitive CO 2 sensing foil based on patent pending fabrication process Sensing patch easy to replace Miniature optical reader with reflector system needs low power Copyright 2017 CSEM Raphaël Pugin Page 13
15 CO 2 sensor performances Absorbance SPECIFICATIONS accuracy and confidence interval (CI) Single point calibration in ambient air 0-15% CO 2 (stable environment) phenol red CO 2 sensing in gas phase at 23 C and 70% humidity Accuracy 0.2% at 3% CO 2 0.2% at 12% CO 2 Precision - 95% confidence interval ±0.4% at 3% CO 2 ±1% at 12% CO 2 Reference spectrometer Copyright 2017 CSEM Raphaël Pugin Page 14
16 Conclusions Process chains for the fabrication of nanostructures surfaces and components have been developed and patented: nanostructured plastic chip by injection molding Mesoporous films by sol-gel process Functional nanostructured sensors have been fabricated for different applications High performance biodiagnostic platform Optical sensor for O 2 monitoring in cell culture device Pressure sensitive painting for aeronautics Optical sensor for CO 2 monitoring (Life Sciences and air quality control) Copyright 2017 CSEM Raphaël Pugin Page 15
17 Thank you for your attention and follow us on
18 Optical gas sensing Sensor performances Accuracy & Precision calibrated gas Gas concentration Gas concentration Gas concentration Gas concentration Copyright 2017 CSEM Raphaël Pugin Page 17
19 3D mold insert structuring
20 Deposition of particles on the microstructured mold insert Objective : deposition of nanoparticles on the mold insert produced by Vuillermoz (in the holes made by micromilling (Ø300µm, depth 150µm)) Successfull deposition at the bottom of the microholes Copyright 2017 CSEM Raphaël Pugin Page 19
21 Deposition of particles on the microstructured mold insert Deposition on the sidewalls of the microholes The background roughness due to the micromilling process does not affect the deposition process Copyright 2017 CSEM Raphaël Pugin Page 20
22 Deposition of particles on freeform parts Deposition on a coronary stent Copyright 2017 CSEM Raphaël Pugin Page 21
23 3D mold insert structuring Use the process developped for 2D parts onto the 3D mold insert. Fabrication of a nanoporous etch mask and electrochemical etching Nanostructures have been fabricated into the microholes Homogeneity to be improved Difficulties to characterize the results (AFM not possible) Copyright 2017 CSEM Raphaël Pugin Page 22
24 Fabrication of nanostructures Origination of nanopatterns by self-assembly Fabrication of a formulation containing micro-nanoparticles or block copolymers and deposition of thin films on a substrate Particles or polymers + solvent +additives Material costs: 20 /100g Formulation/Dispersion Deposition Characterization Copyright 2017 CSEM Raphaël Pugin Page 23
25 Fabrication of nanostructures Random low-cost nanostructures transfered in replication tool Self-Assembled structures transferred in nickel or hard steel replication tools 2 and 3D nanostructured inserts and mold Copyright 2017 CSEM Raphaël Pugin Page 24
26 Fabrication of nanostructures Replication UV Nanoimprint Hot embossing Injection molding Rapid prototyping Highest accuracy UV-curable resins (PUA, solgel) Small series production Thermoplastic & thermosetting materials High throughput Large series production 500nm 1µm 2µm 1µm Copyright 2017 CSEM Raphaël Pugin Page 25
27 Applications Superhydrophobic surfaces Fabrication of micro/nanostructured plastic parts using replication techniques. Control surface chemistry using MVD technology Characterization of wettability : dynamic water contact-angles, high speed videos recording of drop impacts Superhydropbobic, self-cleaning surfaces Controlled wetting states (Wenzel vs Cassie-baxter) Superhydrophilic /hemiwicking surface also possible Chemistry: Perfluoro SAM Copyright 2017 CSEM Raphaël Pugin Page 26
28 Superhydrophobic surfaces o High speed video records of water drops impacts on superhydrophobic surfaces: Flat Structure 1 Structure 2 Copyright 2017 CSEM Raphaël Pugin Page 27
29 Applications Anti-icing surfaces ICEAGE Objective: Fabrication of superhydrophobic surfaces with enhanced erosion resistance to improve the performance of electromechanical de-icers and lower their energy consumption Lift decreases Weight increases Airflow disrupted Drag increases Fabrication of nanostructured surfaces by means of UV-nanoimprint Control surface chemistry using MVD technology Characterization: measurement of ice-adhesion strength, outdoor icing tests Copyright 2017 CSEM Raphaël Pugin Page 28
30 Applications Anti-icing surfaces ICEAGE Both structured/flat samples are iced with snow gun Ice easily removed from nanostructured samples by pole shaking Ice remained on polished Al surface, event after scratching Ice adhesion strength measured by applying shear until the pellet adhesively or cohesively breaks F Copyright 2017 CSEM Raphaël Pugin Page 29
31 Nano-Surface Engineering Application: wearable device Objective: Create and integrate PV solution in a wristband Fabricate textured surface for the growth of the flexible solar cell that provide excellent light coupling capabilities Outstanding PV performance at ultra-low illumination Upscaling of the texturation process (30x30 cm 2 ) successful. UV-NIL on µm thick polymer foil Copyright 2017 CSEM Raphaël Pugin Page 30
32 Applications Nanostructured biodiagnostic platform Objective : Injection molding of nanostructured biodiagnostic platform with improved sensitivity Control of the wettability of detection spots Improve signal quality and homogeneity Tooling: fabrication of nanostructures on a mold insert presenting microchannels and microholes Replication : optimization of the replication process for nanostructured micromolds Copyright 2017 CSEM Raphaël Pugin Page 31
33 Applications Biological cell growth Grow eukaryotic cells on flat and structured surfaces Analyse the morphology of the cells after 3days Characterize the adhesion of cells on flat/structured surfaces Control the growth of adherent cells via micro-nanostructuring surfaces. Create surfaces with cell-adhesion/cell repellent patterns Copyright 2017 CSEM Raphaël Pugin Page 32
34 Functionalisation via aerosol-jet printing Aerosol-jet printer system AJ-300 Aerodynamic focusing of colloidal suspension Contact-free deposition 3D and flexible substrates Deposited materials incl. metals, polymers, ceramics, dielectrics, biomaterials Electrode on pipet tip 2 mm 1 mm Copyright 2017 CSEM Raphaël Pugin Page 33
35 Biofunctionalisation by Aerosol Jet Printing Currently optimising deposition parameters for biological samples on various chemistries Spots can be done on structured or 3D samples Fluorescent BSA spotted on epoxysilane slide (Nexterion Slide E, Schott) Fluorescent BSA spotted on aminosilane slide (Nexterion Slide A+, Schott) Fluorescent BSA spotted on hydrogel slide (Nexterion Slide H, Schott) Average spot size: 79 μm Average spot size: 67 μm Average size: 68 μm Copyright 2017 CSEM Raphaël Pugin Page 34
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