HPLC COLUMNS. HPLC Columns & Bulk Resins THE MEASURE OF EXCELLENCE TM

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1 HPLC COLUMNS & Bulk Resins THE MEASURE OF EXCELLENCE TM

2 The Measure of Excellence Hamilton For nearly 0 years, Hamilton Company has been satisfying customer needs in the field of precision fluid measuring. It all started with syringes. Not commercial, mass produced medical syringes, but precision measuring instruments. About years ago Hamilton was the first company to develop and manufacture pressure stable polymeric HPLC columns. Columns We now offer 9 different polymer-based HPLC columns for reversed phase, anion exchange, cation exchange and ion exclusion separations. Two silica-based C8 and C8 columns are available for reversed phase separations. Choosing the Right HPLC Column Before you select an HPLC column to separate your sample please check the Hamilton Company HPLC web site at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated on Hamilton HPLC columns.

3 This brochure only contains a few of the applications which have been performed on Hamilton HPLC columns. In the brochure, columns are grouped by separation mechanism: reversed phase, anion exchange, cation exchange and ion exclusion. The Recommended Uses Table below lists a few of the possible uses for each of the column packings. Recommended Uses Reversed Phase PRP -h Long life column for LC/MS applications,synthesized DNA, and small molecule PRP-h PRP- PRP- PRP-Infinity HxSil C8, C8 Reduced system pressure for protein and peptide separations and enhanced oligonucleotide recovery General purpose ph stable long life column, synthesized DNA Gradient protein and peptide separations Nonporous support for very fast gradient separation of large proteins General purpose silica-based reversed phase Anion Exchange PRP-X00 Anions, inorganic and organic using conductivity or UV detection. 0 to 00% solvent compatible. PRP-X0 PRP-X00 PRP-X600 RCX-0 RCX-0 Similar to PRP-X00 but for lower level anions (0 ppb to 0 ppm) Gradient separation of large proteins and labeled DNA Gradient separation of labeled and unlabeled DNA Isocratic or gradient separation of carbohydrate oligomers up to DP8 Gradient separation of complex carbohydrates Cation Exchange PRP-X00 Inorganic and organic cations using conductivity or UV detection. Separate mono or divalent cations depending on mobile phase conditions. PRP-X00 PRP-X800 HC-0 HC-7 HC-7 HC-7 Glyphosate and metabolite in drinking water. Also unique hydrophilic interaction separations Mono and divalent cations in the same run. Transition metals Sugar oligomers up to DP8. Max pressure,000 psi Mono and disaccharides in corn syrup. Max pressure,000 psi Calcium Form Organic acids and sugars. Max pressure,000 psi Hydrogen Form Sugar alcohols. Max pressure,000 psi Lead Form Ion Exclusion PRP-X00 Organic acids and alcohols

4 USP L Numbers & Bulk Resins USP "L" Number and Hamilton USP "L" Number HAMILTON Column L L7 L7 L9 L L L Octadecylsilane chemically bonded to porous silica or ceramic micro-particles, to 0 µm in diameter. Octylsilane chemically bonded to porous silica particles, to 0 µm in diameter. Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to µm in diameter. Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, about 9 µm in diameter. A rigid, spherical styrene-divinylbenzene copolymer, to 0 µm in diameter. An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, about 0 µm in size. Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead form, about 9 µm in diameter. HxSil C8 HxSil C8 HC-7 Hydrogen Form HC-7 Calcium Form PRP- PRP- PRP-X00 HC-7 Lead Form Bulk Resins Reversed Phase Support Exchange Pore Supports Material Capacity Size µm µm 7 µm 0 µm -0 µm 0-0 µm 0-7µm PRP-h PSDVB* N/A 00 Å 7979 PRP-h PSDVB* N/A 00 Å 7980 PRP- PSDVB* N/A 00 Å PRP- PSDVB* N/A 00 Å HxSil C8 Silica HxSil C8 Silica Anion Exchange Support Exchange Pore Supports Material Capacity Size µm µm 7 µm 0 µm -0 µm 0-0 µm 0-7µm PRP-X00 PSDVB* with Trimethyl- 0.9 meq/gm 00 Å ammoniun Exchanger PRP-X00 Poly(methacryl-.6 meq/gm Superfi amidopropryl Trimethyl- cially ammonium chloride porous PRP-X600 Poly(dimethyl-.6 meq/gm Superfi amidopropyl-methacryl- cially amide) porous Cation Exchange Support Exchange Pore Supports Material Capacity Size µm µm 7 µm 0 µm -0 µm 0-0 µm 0-7µm PRP-X00 PSDVB* with Sulfonate µeq/gm 00 Å Exchanger PRP-X00 PSDVB* with Sulfonate. meq/gm N/A Exchanger * PSDVB is Poly(styrene-divinylbenzene) Bulk resin is sold by the gram.

5 PRP - Reversed Phase Reversed Phase PRP -h Polymeric 00 Å columns for reversed phase, LC/MS applications. ph stable from to. Low bleed (ideal for mass spectrometry). Robust (virtually any HPLC solvent can be used). One particle size: µm. Four column diameters:.,.6, 0 and 00 mm. Analytical and semiprep/preparative guard columns. Benzoic Acid and its Derivatives PRP-h, µ, 00 Å,. x 0 mm.,-dihdroxybenzoic Acid. -Hydroxybenzoic Acid. -Acetoxybenzoic Acid (Aspirin). Benzoic Acid. - Hydroxybenzoic Acid (Salicylic Acid) Naproxen Sodium PRP-h, µm, 00 Å,. x 0 mm,. Naproxen (0 µg/ml). Ibuprofen (80 µg/ml) I.D. 0 mm 00 mm 0 mm 0 mm. mm mm mm mm Mobil Phase: A: 0 mmol/l sodiumdihydrogenphosphate ph =. B: Acetonitrile. Gradient: 8% B (Isocratic) Flow Rate:0.60 ml/min. Column Temperature: 0 o C Detection: nm. Injection Volumn: µl Analytical Guard Columns Starter Kit 797 ( holder, cartridges) Replacement Cartridges (/pk) 798 * Analytical guard column for steel columns is. x 0 mm Semiprep/Preparative Guard Column Starter Kit 797 ( holder, cartridge) Replacement Cartridges (/pk) 7976 *** Semiprep/preparative guard column for steel columns is.6 x 0 mm 0 Mobil Phase: A: 0 mmol/l Sodiumdihydrogenphosphate ph = B: Acetonitrile. Gradient: Isocratic 0% A/0%B Flow Rate:0.60 ml/min. Column Tempera ture: 60 o C Detection: nm. For Steel Columns* For Steel Columns***

6 PRP-h Reversed Phase Polymeric 00 Å columns for oligonucleotide purification and protien separations. ph stable from to. Robust (virtually any HPLC solvent can be used). Hydrophilic polymer structure delivers faster oligonucleotide purification. -mer Oligonucleotide Purification PRP-h, PRP-h,. x µ, 0 00 mm, Å,. µm x 0 00 mm Å One particle size: µm. Three column diameters:.,.6 and 0 mm. Analytical and semiprep/preparative guard columns Mobile phase: A: 0. mol/teaa,ph = 7 B: 0. mol/l TEAA, ph = 7, % Acetonitrile Gradient: 0-80 %B in min. Flow Rate: 0.60 ml/min Column Temp: 60 o C Detection: 60 nm I.D. 0 mm 00 mm 0 mm 0 mm. mm mm mm Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated. Analytical Guard Columns Starter Kit 7967 ( holder, cartridges) Replacement Cartridges (/pk) 7968 * Analytical guard column for steel columns is. x 0 mm Semiprep/Preparative Guard Column Starter Kits 7977 ( holder, cartridge) Replacement Cartridges (/pk) 7978 *** Semiprep/preparative guard column for steel columns is.6 x 0 mm For Steel Columns* For Steel Columns***

7 PRP- Reversed Phase Polymeric 00 Å columns for general reversed phase separations. ph stable from to. Better sample recovery than silica-based columns (there are no silanol groups). Excellent durability (stable to any concentration of water or organic solvent). Five particle sizes:, 7, 0, -0 and 0-0 µm. Eleven column internal diameters:.0 to 0.6 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. Small Molecules DNA with Secondary Structure Biocides found in Soap PRP-,. x 0 mm, µm (P/N 7980) PRP-,. x 0 mm, 0 µm (P/N 79) PRP-,. x 0 mm, 7 µm (P/N 79). Cytosine. Uracil. Uridine. Failure Sequences. -DMT 6mer. Chloroxylenol 00 ppm. Triclocarban 00 ppm Seconds Conditions: 0.0M Citric Acid ph.. Isocratic. Ambient. ml/min. 0 µl, UV nm Conditions: A) 0mM Sodium Hydroxide ph.7; B) : 0mM Sodium Hydroxide : Acetonitrile. Linear Gradient 0% B (0- min), 0-0% B (- min). 60 C. ml/min. UV 60nm Conditions: : Acetonitrile : Water. Isocratic. Ambient. ml/min. 0 µl, UV nm. 6

8 PRP- Reversed Phase Surfactant Surfactant Fatty Acids PRP-,. x 0 mm, 0 µm (P/N 79) PRP-,. x 0 mm, 0 µm (P/N 79) PRP-,. x 00 mm, µm (P/N 7980). Sodium Octadecylsulfate 00 ppm. Sodium Dodecylsulfate 00 ppm. Caproic Acid. Caprylic Acid. Capric Acid. Lauric Acid. Palmitic Acid 6. Stearic Acid 6 0 Conditions:.:. Acetonitrile : Deionized Water. Isocratic. Ambient. ml/min. 0 µl, Conductivity Conditions: :7 Acetonitrile : Deionized Water. Isocratic. Ambient. ml/min. 0 µl, Conductivity 0 0 Conditions: A) : Acetonitrile : Water; B) Acetonitrile. Linear Gradient 0-00% B in min. Hold for min. Ambient. 0. ml/min. 0 µl, UV nm. Isomeric C8 Fatty Acids Chlorhexidine Gluconate Resveratrol PRP-,. x 0 mm, 7 µm (P/N 79) PRP-,. x 0 mm, 0 µm (P/N 79) PRP-,. x 0 mm, 7 µm (P/N 79). Linolenic Acid (C8:). Linoleic Acid (C8:). Oleic Acid (C8:). Stearic Acid (C8:0). Chlorhexidine Gluconate µg. Resveratrol Conditions: 0.% Trifluoroacetic Acid in 9: Acetonitrile : Water. Isocratic. Ambient. 0.8 ml/min. 00 µl, UV 0 nm. 0 6 Conditions: :0:69 Trifluoroacetic Acid : Acetonitrile : Deionized Water. Isocratic. Ambient. ml/min. µl, UV nm Conditions: :7 Acetonitrile : 0.M Sodium Dihydrogen Phosphate ph.. Isocratic. Ambient. ml/min. 0 µl, UV 80 nm. Pyridinoline Diphenylguanidine Folic Acid PRP-,. x 0 mm, 0 µm (P/N 79) PRP-,. x 0 mm, 0 µm (P/N 79) PRP-,. x 0 mm, 7 µm (P/N 79). Pyridinoline, mg/ml. Diphenylguanidine mg/ml. Folic Acid 0 Conditions: :9 Acetonitrile : Deionized Water with 0.% Heptafluorobutyric Acid. Isocratic. Ambient. ml/min. µl, UV 80 nm. 0 Conditions: :8 Acetonitrile : Deionized Water with 0. % Trifluoroacetic Acid. Isocratic. Ambient. ml/min. 0 µl, UV 80 nm. 0 0 Conditions: 97.:. 0mM Sodium Phosphate ph 6. : Acetonitrile. Isocratic Ambient. ml/min. µl, UV 80 nm. 7

9 PRP- Reversed Phase Pregnenolone and Pregnenolone--sulfate Water Soluble Vitamins Fat Soluble Vitamins PRP-,. x 0 mm, 7 µm (P/N 79). Pregnenolone--sulfate. Pregnenolone PRP-,. x 0 mm, 0 µm (P/N 79). Niacinamide. Pyridoxine (B6). Thiamine (B). Riboflavin (B). Cyanocobalamine (B) PRP-,. x 0 mm, 7 µm, (P/N 79). Retinol (Vitamin A) 00 ppm. Calciferol (Vitamin D) 0 ppm. alpha-tocopherol (Vitamin E) 0 ppm. Phylloquinone (Vitamin K) 0 ppm 0 0 Conditions: 7: Acetonitrile : mm Tetrabutylammonium Hydrogensulfate. Isocratic. Ambient. ml/min. 0 µl, UV 0 nm Conditions: A) 0.N Perchloric Acid; B) Acetonitrile. Linear Gradient -0%B in 0 min. Ambient. ml/min. 0 µl, UV 0 nm Conditions: 0:70:0 Tetrahydrofuran : Acetonitrile : Water. Isocratic. Ambient.. ml/min. 00 µl, UV 80 nm. I.D. x Length µm 7 µm 0 µm -0 µm.0 x 0 mm x 00 mm x 0 mm x 0 mm x 0 mm x 00 mm x 0 mm x 0 mm* x 0 mm x mm 79789**. x 0 mm 79. x 00 mm x 0 mm x 0 mm x 0 mm* x 00 mm* x 0 mm* * PEEK hardware ** Cartridge Column, Cartridge Holder P/N 908 must be purchased separately. I.D. x Length µm 7 µm 0 µm -0 µm 0-0 µm.6 x 0 mm* x 00 mm x 00 mm x 0 mm x 0 mm x 00 mm x 0 mm x 0 mm x 00 mm x 0 mm x 0 mm x 00 mm x 00 mm x 0 mm x 0 mm x 0 mm 79 Analytical Guard Columns For Steel Columns* For PEEK Columns** Semiprep/Preparative Guard Column For Steel Columns*** Starter Kits Starter Kit 79 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) Replacement Cartridges (/pk) 79 * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm *** Semiprep/preparative guard column for steel columns is.6 x 0 mm Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated. 8

10 PRP- Reversed Phase Polymeric 00 Å columns for protein separations. ph stable from to. Better sample recovery than silica-based columns (there are no silanol groups). Excellent durability (the support can be cleaned with strong acid or base to remove any sample residue). Two particle sizes: 0 and -0 µm. Eight column internal diameters:.0 to 0.8 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. Proteins PRP-,. x 0 mm, 0 µm (P/N 7966). Ribonuclease A. Cytochrome c. Lysozyme. Myoglobin. Ovalbumin 0 8 Conditions: A) 0.% TFA in Water ph.0; B) 0.% TFA in Acetonitrile. Linear Gradient -0% B in min. Hold min. Ambient. ml/min. 00 µl, UV nm. Proteins at ph. PRP-,. x 0 mm, 0 µm (P/N 7966) Ribonuclease A.0 mg/ml. Insulin.0 mg/ml. Cytochrome c.0 mg/ml. Trypsin.0 mg/ml. Lysozyme.0 mg/ml 6. Pyruvate Dehydrogenase 0. mg/ml Conditions: A) 0.% TFA in 0mM Sodium Hydroxide ph.; B) 0.% TFA in Acetonitrile. Linear Gradient 0-60% B in 0 min. Ambient. ml/min. 0 µl, UV 0 nm. I.D. x Length 0 µm.0 x 0 mm x 00 mm x 0 mm x 0 mm x 0 mm. x 00 mm x 0 mm 799. x 0 mm x 00 mm I.D. x Length 0 µm -0 µm. x 0 mm x 0 mm x 0 mm* x 0mm* x 0 mm* x 0 mm x 0 mm 796. x 0 mm x 0 mm 7987 * PEEK hardware Analytical Guard Columns For Steel Columns* For PEEK Columns** Semiprep/Preparative Guard Column For Steel Columns* Starter Kits Starter Kit 79 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) Replacement Cartridges (/pk) 79 9 * Analytical guard column for steel columns is. x 0 mm * Semiprep/preparative guard column for steel columns is.6 x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm

11 PRP-Infinity Reversed Phase Polymeric nonporous packing for fast gradient separation of large proteins. ph stable from to. Better sample recovery than silica-based columns (there are no silanol groups). Excellent durability (the support can be cleaned with strong acid or base to remove any sample residue). One particle size: µm. Three column internal diameters:. to 0.0 mm. One column material: 6 stainless steel. Proteins PRP-Infinity,. x mm, µm (P/N 7970). Ribonuclease A. Cytochrome c. Transferrin. Bovine Serum Albumin. Concanavalin A 6. Ovalbumin 6 Proteins PRP-Infinity,. x mm, µm (P/N 7970) 6. Ribonuclease A. Cytochrome c. Transferrin. Bovine Serum Albumin. Concanavalin A 6. Ovalbumin I.D. x Length µm. x mm x 00 mm x mm x 0 mm x 60 mm 797 For PRP-Infinity columns an in-line filter is recommended as a guard column is not available. 0 0 Conditions: A) 0.% TFA in Water ph.0; B) 0.% TFA in Acetonitrile. Linear Gradient 0-60% B in 0 min. Ambient. ml/min. 0 µl, UV nm Conditions: A) 0.% TFA in Water ph.0; B) 0.% TFA in Acetonitrile. Linear Gradient -60% B at 0.6% per second. Ambient. ml/min. 0 µl, UV nm. Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated. 0

12 HxSil C8 & C8 Reversed Phase Silica-based 00 Å columns for general reversed phase separations. Separation of closely related PAHs. Better retention of poorly retained compounds. Separation of neutral, acidic and basic compounds without tailing (tailing factor of less than.0). Two particle sizes: and µm. Five column internal diameters:. to. mm. Analytical and semiprep/preparative guard columns in 6 stainless steel. Sunscreens in Lip Balm Sunscreen Compounds Diphenhydramine in Itch Relief Gel HxSil C8,.6 x 0 mm, µm (P/N 79868). Oxybenzone. Octocrylene. Octyl Methoxycinnamate. Avobenzone (Parsol 789). Octyl Salicylate HxSil C8,.6 x 0 mm, µm (P/N 79868). -Hydroxy-- methoxybenzophenone. -Ethylhexyl trans-- methoxycinnamate. -Ethylhexylsalicylate HxSil C8,.6 x 0 mm, µm (P/N 79868). Diphenhydramine 0 ppm Conditions: 8: Methanol : Water. Isocratic. Ambient. ml/min. ul, UV 08 nm. 0 0 Conditions: 7: Acetonitrile : Water. Isocratic. Ambient. ml/min. 0 µl, UV nm. 0 Conditions: 9: Methanol : 0mM Potassium Hydrogen Phosphate ph 7.0. Isocratic. Ambient. ml/min. µl, UV nm. Meclizine in Tablets Tetracycline by USP Method Oxytetracycline and Tetracycline HxSil C8,.6 x 0 mm, µm (P/N 79868) HxSil C8,.6 x 0 mm, µm (P/N 79869) HxSil C8,.6 x 0 mm, µm (P/N 790). Meclizine mg/ml. Tetracycline 0. mg/ml. Oxytetracycline 0. mg/ml. Tetracycline 0. mg/ml 0 0 Conditions: 9: Methanol : 0mM Potassium Hydrogen Phosphate ph 7.0. Isocratic. Ambient. ml/min. µl, UV nm Conditions: 680 ml 0.N Ammonium Oxalate, 70 ml Dimethylformamide, 0 ml 0.M Dibasic Ammonium Phosphate ph 7.6. Isocratic. Ambient. ml/min. 0 µl, UV 80 nm. 0 0 Conditions: 680 ml 0.N Ammonium Oxalate, 70 ml Dimethylformamide, 0 ml 0.M Dibasic Ammonium Phosphate ph 7.6. Isocratic. Ambient. ml/min. 0 µl, UV 80 nm.

13 HxSil C8 & C8 Reversed Phase Folic Acid, Methotrexate by USP Method Antibiotics Small Molecules HxSil C8,.6 x 0 mm, µm (P/N 79868) 0 0 Conditions: 9: 0.6M Sodium Phosphate, Dibasic, 0.07M Citric Acid ph 6.0 : Acetonitrile. Isocratic. Ambient.. ml/min. 0 µl, UV 0 nm.. Folic Acid 00 µg/ml. Methotrexate 00 µg/ml HxSil C8,.6 x 0 mm, µm (P/N 790). Ampicillin. Oxacillin. Dicloxacillin 0 0 Conditions: : 0.0M Potassium Phosphate ph 7 : Acetonitrile. Isocratic. Ambient. ml/min. µl, UV nm. HxSil C8,.6 x 0 mm, µm (P/N 79868) 6. Uracil. Caffeine. Phenol. Toluene. Butylbenzene 6. Triphenylene 7. Amylbenzene 8. Terphenyl Conditions: 7: Acetonitrile : Water. Isocratic. Ambient. ml/min. 0 µl, UV nm. 7 8 HxSil C8 I.D. x Length µm µm. x mm 796. x 0 mm x 7 mm x 00 mm x 0 mm x 0 mm 79.6 x mm 79 I.D. x Length µm µm.6 x 0 mm x 00 mm x 0 mm x 0 mm x 0 mm x 0 mm 790. x 0 mm 7906 Analytical Guard Column For Steel Columns* Semiprep/Preparative Guard Column For Steel Columns* Starter Kit 798 Starter Kit 79 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) 79 Replacement Cartridges (/pk) 796 * Analytical guard column for steel columns is. x 0 mm * Semiprep/preparative guard column for steel columns is.6 x 0 mm HxSil C8 I.D. x Length µm µm. x mm x 0 mm x 7 mm x 00 mm x 0 mm x 0 mm x mm I.D. x Length µm µm.6 x 0 mm x 00 mm x 0 mm x 0 mm x 0 mm x 0 mm x 0 mm 7987 Analytical Guard Column For Steel Columns* Semiprep/Preparative Guard Column For Steel Columns* Starter Kit 799 Starter Kit 797 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) 79 Replacement Cartridges (/pk) 798 * Analytical guard column for steel columns is. x 0 mm * Semiprep/preparative guard column for steel columns is.6 x 0 mm Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated.

14 Anion Exchange PRP-X00 Polymeric anion exchange packings for separation of inorganic and organic anions. Easily separate the eight common anions (fluoride through sulfate). Good separation of fluoride from the water dip. Use with organic solvent from 0 to 00 % for elution of hydrophobic anions or column cleaning. ph stable from to. Use conductivity or UV detection. Three particle sizes:, 0 and -0 µm. Eight column internal diameters:.0 to 0.8 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. Common Anions Anions by Indirect UV Detection EDTA PRP-X00,. x 0 mm, µm (P/N 7980). Fluoride. Chloride. Nitrite. Bromide. Nitrate PRP-X00,. x 0 mm, 0 µm (P/N 79). Fluoride 0 ppm. Carbonate 0 ppm. Chloride 0 ppm. Nitrite 0 ppm. Bromide 0 ppm 6. Nitrate 0 ppm 7. Phosphate 0 ppm 8. Sulfate 0 ppm PRP-X00,.6 x 0 mm, 0 µm (P/N 79). Copper EDTA 0 ppm Conditions: mm p-hydroxybenzoic Acid ph 8. w/.% Methanol ml/min. 00 µl, Conductivity Conditions: mm p-hydroxybenzoic Acid ph 8.9 with. % Methanol. Isocratic. Ambient. ml/min. 00 µl, Indirect UV 0 nm. 0 6 Conditions: mm Sulfuric Acid. Isocratic. Ambient. ml/min. 00 ul, UV nm.

15 PRP-X00 Anion Exchange Organic Acids Germall Bromate and Other Anions PRP-X00,. x 0 mm, 0 µm (P/N 79). Formic Acid 0 µg. Fluoroacetic Acid 0 µg. Isopropylmethyl Phosphonic Acid 0 µg. Chloroacetic Acid 0 µg PRP-X00,.6 x 0 mm, 0 µm (P/N 79),. Germall mg/ml PRP-X00,. x 0 mm, 0 µn (P/N 79). Fluoride 0 ppm. Chloride 0 ppm. Bromate 0 ppm. Bromide 0 ppm. Nitrate 0 ppm 0 0 Conditions: 0.M Sodium Hydroxide in :9 Acetonitrile : Deionized Water. Isocratic. Ambient. ml/min. 0 µl, Conductivity. 0 0 Conditions: 7: 0mM Sodium Hydroxide : Methanol. Isocratic. Ambient. ml/min. 0 µl, UV 0 nm Conditions:.7mM Sodium Bicarbonate,.8mM Sodium Carbonate, 0.mM Sodium Thiocyanate. Isocratic. Ambient. ml/min. 0 µl, Suppressed Conductivity. Sulfite and Sulfate Phosphate and Phosphite Chlorite and Chlorate PRP-X00,.6 x 0 mm, 0 µm (P/N 79). Sulfite.7 mg/ml. Sulfate 0. mg/ml PRP-X00,. x 0 mm, 0 µm (P/N 79). Fluoride 0 ppm. Phosphate 0 ppm. Phosphite 0 ppm. Chloride 0 ppm PRP-X00,. x 0 mm, 0 µm (P/N 79) Fluoride. Chlorite. Chloride. Nitrite. Phosphate 6. Bromide 7. Nitrate 8. Chlorate 0 6 Conditions: 80:0 8mM p-hydroxybenzoic Acid ph 0.0 : Methanol. Isocratic. Ambient. ml/min. 0 µl, Conductivity Conditions: 0mM Succinic Acid ph.9. Isocratic. Ambient. ml/min. 00 µl Conductivity Conditions:.0mM -Amino--Hydroxybenzoic Acid ph.8. Isocratic. Ambient. ml/min.00 µl, Indirect UV 0 nm. I.D. x Length µm 0 µm.0 x 0 mm x 00 mm x 0 mm x 0 mm x 0 mm x 0 mm 79. x 0 mm* x 0 mm x 0 mm 796. x 00 mm x 0 mm 79 I.D. x Length µm 0 µm -0 µm. x 0 mm x 0 mm* x 0 mm* x 0 mm x 0 mm x 0 mm 79. x 00 mm 79. x 0 mm 79. x 0 mm x 0 mm 79 PEEK hardware Analytical Guard Columns For Steel Columns** For PEEK Columns*** Semiprep/Preparative Guard Column For Steel Columns**** Starter Kits Starter Kit 79 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) Replacement Cartridges (/pk) 796 ** Analytical guard column for steel columns is. x 0 mm *** Analytical guard column for PEEK columns is.0 x 8.0 mm **** Semiprep/preparative guard column for steel columns is.6 x 0 mm

16 PRP-X0 Anion Exchange Polymeric anion exchange packings for separation of inorganic and organic anions from 0 ppb to 0 ppm. Good separation of fluoride from the water dip. Compatible with conductivity or UV detectors. Use with organic solvent up to 00% ph stable from to. Anions One particle size: 7 µm. Four column internal diameters:.0 to.6 mm. Polarizable Anions Two column materials: 6 stainless steel and PEEK. Analytical guard columns. Glucaric Acid PRP-X0,. x 0 mm, 7 µm (P/N 797) 6 7. Fluoride ppm. Chloride ppm. Nitrite ppm. Bromide ppm. Nitrate ppm 6. Phosphate ppm 7. Sulfate ppm PRP-X0S,. x 0 mm, 7 µm (P/N 797) 6. Fluoride ppm. Chloride ppm. Iodide 0 ppm. Sulfate 0 ppm. Thiosulfate 0 ppm 6. Perchlorate 0 ppm PRP-X0,. x 0 mm, 7 µm (P/N 797). Glucaric Acid 000 ppm 0 8 Conditions:.0mM p-hydroxybenzoic Acid ph 9.. Isocratic. Ambient. ml/min. 00 µl, Non-Suppressed Conductivity. 0 7 Conditions: mm Sodium Hydroxide with mm Phenol, 0.0mM Sodium Thiocyanate. Ambient.. ml/min. 0 µl, Suppressed Conductivity. 0 6 Conditions: :7 Acetonitrile : 0mM Potassium Phosphate, ph 6.. Isocratic. Ambient.. ml/min. 0 µl, UV 0 nm. PRP-X0 PRP-X0S I.D. x Length 7 µm 7 µm.0 x 0 mm x 00 mm x 0 mm x 0 mm x 00 mm* x 0 mm* x 0 mm* x 00 mm x 0 mm x 0 mm PRP-X0 PRP-X0S I.D. x Length 7 µm 7 µm.6 x 00 mm* x 0 mm* x 0 mm* * PEEK hardware Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm

17 PRP-X00 Anion Exchange Polymeric strong-base anion exchange packing with limited porosity for separation of proteins. Fast separation of large proteins. ph stable from to. One particle size: 7 µm. Three column internal diameters:. to 0.0 mm. Two column materials: 6 stainless steel and PEEK. Higher sample capacity than nonporous supports. DMT-on Antisense Phosphorothioate 8mer PRP-X00,.6 x 0 mm, 7 µm (P/N 797). DMT-on Phosphorothioate 8mer Proteins PRP-X00,.6 x 0 mm, 7 µm (P/N 797). Myoglobin 7 µg. Conalbumin 7 µg. Dog Albumin 77 µg Conditions: A) 8: 00mM TRIS ph 8.0 : Acetonitrile; B) 8: 00mM TRIS ph.0,.m Lithium Chloride : Acetonitrile. Linear Gradient 0-00% B in 0 min. ml/min. 00 µl of a mg/ml solution, UV 60nm. 0. Conditions: A) 0mM Tris ph 9.0; B) 0mM Tris ph 9.0, 0.N Sodium Chloride. Linear Gradient 0-0% B in. min. Hold for. min. Ambient. ml/min. 0 µl, UV nm. I.D. x Length 7 µm. x 0 mm* x 0 mm 79.6 x 0 mm* x 0 mm* x 00 mm 798 Analytical Guard Column For PEEK Columns* Starter Kit ( holder, cartridges) 799 Replacement Cartridges (/pk) 790 * Analytical guard column for PEEK columns is.0 x 8.0 mm * PEEK hardware 6

18 PRP-X600 Anion Exchange Polymeric weak-base anion exchange packing for gradient separation of proteins and DNA oligomers. Limited porosity allows separation of large DNA fragments. ph stable from to. One particle size: 7 µm. Column material: PEEK. Guard column in PEEK. Higher sample capacity than nonporous supports. Separation of DNA Fragments DMT-on Phosphorothioate 8mer Proteins PRP-X600,.6 x 0 mm, 7 µm (P/N 7960) PRP-X600,.6 x 0 mm, 7 µm (P/N 7960). DMT-on Phosphorothioate 8mer PRP-X600,.6 x 0 mm, 7 µm (P/N 7960). Myoglobin mg/ml. Concalbumin mg/ml. Ovalbumin mg/ml. Bovine Serum Albumin mg/ml Conditions: A) 0mM TRIS, mm EDTA ph 9.0; B) N NaCl in A, Linear Gradient % B in min. 67.%-7% B (- min.). Ambient. ml/min. µl, UV 60 nm Conditions: A) 8: 00mM TRIS ph 8.0 : Acetonitrile; B) 8: 00mM TRIS ph.0,.m Lithium Chloride : Acetonitrile. Linear Gradient 0-00% B in 0 min. ml/min. UV 60 nm. 0 7 Conditions: A) 0mM TRIS ph 9.0; B) 0mM TRIS, ph 9.0,.0M Sodium Chloride. Linear Gradient 0 to %B in 0 min. Hold min. ml/min. UV 0nm. 7 I.D. x Length 7 µm.6 x 0 mm* x 00 mm* x 0 mm* x 0 mm* 7989 * PEEK hardware Analytical Guard Column For PEEK Columns* Starter Kit ( holder, cartridges) 796 Replacement Cartridges (/pk) 796 * Analytical guard column for PEEK columns is.0 x 8.0 mm Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated.

19 RCX-0 Anion Exchange Polymeric anion exchange packing for isocratic or gradient separation of carbohydrates. ph stable from to. Isocratic separation of mono and disaccharides. Gradient separation of oligomers up to DP8. Compatible with PAD, conductivity and RI detectors. One particle size: 7 µm. Three column internal diameters:. to.6 mm. Two column materials: 6 stainless steel and PEEK. Guard columns in stainless steel and PEEK. Glycols in Water Acetyl Glucosamine Artichoke Tubers RCX-0,. x 0 mm, 7 µm (P/N 790). Ethylene Glycol 00 ppb. Propylene Glycol 00 ppb 0 8 Conditions: 0mN Sodium Hydroxide. Isocratic. Ambient. ml/min. 0 µl, Pulsed Amperometric, Dual Gold Electrode. E=60 mv T=66 msec E=900 mv T=66 msec E=-800 mv T=00 msec RCX-0,. x 0 mm, 7 µm (P/N 790). Galactosamine 00 ppm. Glucosamine 00 ppm. Acetyl-Glucosamine 00 ppm 0 7 Conditions: 0mN Sodium Hydroxide (purged with Helium during the run) Isocratic. Ambient. ml/min. 0 µl, Refractive Index. RCX-0,. x 0 mm, 7 µm (P/N 790). DP. DP. DP0. DP 0 0 Conditions: A) 60mM Sodium Hydroxide; B) 60mM Sodium Hydroxide with 00mM Sodium Acetate. Gradient: 0-00%B in 0 min. ml/min. 0 µl, Pulsed Amperometric, Dual Gold Electrode E=00 mv T= msec E=800 mv T=66 msec E=-600 mv T=99 msec I.D. x Length 7 µm. x 0 mm x 0 mm x 0 mm* 7988 Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm 8

20 RCX-0 Anion Exchange Polymeric anion exchange packing for isocratic or gradient separation of complex carbohydrates. ph stable from to. Isocratic or gradient modes. Higher ion exchange capacity support for separation of closely related carbohydrates. Compatible with PAD and RI detectors. One particle size: 7 µm. Three column internal diameters:.,. and.6 mm. Two column materials: Stainless steel and PEEK. Guard column in PEEK. Glycoprotein Monosaccharides Corn Syrup Artichoke Tubers RCX-0,.6 x 0 mm, 7µm (P/N 7970). Fucose. Galactosamine. Glucosamine. Galactose 6. Glucose 6. Mannose RCX-0,.6 x 0 mm (P/N 7970) Glucose. Fructose. Maltose. Maltotriose. Maltotetraose 6. Maltopentaose 7. Maltohexaose 8. Maltoheptaose 9. Maltooctaose RCX-0,.6 x 0 mm (P/N 7970). Glucose. Fructose. Sucrose. DP. DP0 6. DP Conditions: 6mN Sodium Hydroxide. Isocratic. Ambient. ml/min. µl, Pulsed Amperometric µafs Dual Gold Electrode E=600 mv T=66 msec E=600 mv T=66 msec E=-800 mv T=9 msec Conditions: A) 60mM Sodium Hydroxide; B) 00mM Sodium Acetate in A. Linear Gradient 00% A for min., then 0-00% B (- min.) Ambient. ml/min. 0 µl. Pulsed Amperometric Dual Gold Electrode. E=0 mv T=66 msec E=900 mv T=66 msec E=-80 mv T=00 msec Conditions: A) 60mM Sodium Hydroxide; B) 00mM Sodium Acetate in A. Linear Gradient: 0-0% B in 0 min., then 0-00% B (0-0 min.). Ambient. ml/min. 0 µl, Pulsed Amperometric Dual Gold Electrode. E=0 mv T=66 msec E=900 mv T=66 msec E=-800 mv T=00 msec 9 I.D. x Length 7 µm Analytical Guard Column For PEEK Columns*. x 0 mm 7970 Starter Kit ( holder, cartridges) 797. x 0 mm 7980 Replacement Cartridges (/pk) x 0 mm* 7970 * Analytical guard column for PEEK columns is.0 x 8.0 mm.6 x 0 mm* * PEEK hardware Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated.

21 Cation Exchange PRP-X00 Polymeric cation exchange packing for separation of inorganic cations and organic cations. Easily separate mono or divalent cations. ph stable from to. Use with organic solvent up to 00% for elution of hydrophobic cations and column cleaning. Compatible with conductivity and UV detectors. One particle size: 0 µm. Six column internal diameters:.0 to 0.0 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. Monovalent Cations Glycine and Ethanolamine N-Methylglucamine PRP-X00,. x 0 mm (P/N 79). Lithium. Sodium. Ammonium. Potassium. Cesium PRP-X00,. x 0 mm (P/N 79). Glycine. Sodium. Ethanolamine PRP-X00,. x 0 mm (P/N 79). N-Methylglucamine mg/ml 0 0 Conditions:.: mm Nitric Acid: Methanol. Isocratic. Ambient. ml/min. 00 µl, Conductivity. 0 6 Conditions: mn Nitric Acid. Ambient. Isocratic. ml/min. 0 µl, Conductivity. 0 8 Conditions: mn Nitric Acid. Isocratic. Ambient. ml/min. µl, Conductivity. I.D. x Length 0 µm I.D. x Length 0 µm.0 x 0 mm x 0 mm 79.0 x 00 mm x 0 mm 79.0 x 0 mm x 0 mm* 79.0 x 0 mm x 0 mm* 798. x 0 mm x 0 mm* 797. x 0 mm x 0 mm x 00 mm x 0 mm 799 * PEEK hardware * PEEK hardware Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) Semiprep/Preparative Guard Column Starter Kits ( holder, cartridge) 797 Replacement Cartridges (/pk) 798 * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 m *** Semiprep/preparative guard column for steel columns is.6 x 0 mm For Steel Columns*** 0

22 PRP-X00 Cation Exchange Polymeric cation exchange packing for separation of glyphosate and its metabolite in drinking water. Faster analysis when compared to competitor s columns. Detection levels of less than 0 ppb when using the post column, OPA derivatization technique. Applicable to other separations, such as inositol and sugar alcohols. Two particle sizes: 7 and -0 µm. Five column internal diameters:. to. mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. Glyphosate and Metabolite Inositol in Vitamin Matrix Sugar Alcohols PRP-X00,. x 0 mm, 7 µm (P/N 797). Glyphosate. Aminomethylphosphonic Acid PRP-X00,. x 0 mm, 7 µm (P/N 797),,, and. Unknown. Inositol PRP-X00,. x 0 mm, 7 µm (P/N 797). Adonitol 00 ppm. Arabitol 00 ppm. Xylitol 00 ppm. Mannitol 00 ppm. Sorbitol 00 ppm 0 6 Conditions: 0.00M Monobasic Potassium Phosphate. Isocratic. Ambient. 0. ml/min. 00 µl, Excitation wavelength 8 nm. Emission wavelength nm. Post Column Conditions: Oxidation Solution Flow Rate: 0.0 ml/min; Reaction Coil: ml (0.0 cm ID X m); Reaction Time:. min; Temp: 8 C Derivatization Solution: 0.0 ml/min; Reaction Coil: 0.0 ml (0.0 cm ID X m), Ambient. Conditions: 8: Acetonitrile : 0mN Sodium Hydroxide. Isocratic. Ambient. ml/min. 00 µl, Pulsed Amperometric Dual Gold Electrode. E=00 mv T=76 msec E=000 mv T=66 msec E=-800 mv T=99 msec Post Column Conditions: 0.N Sodium Hydroxide solution at ml/min Conditions: A) Acetonitrile; B) Water. Linear Gradient 0-0% B in 0 min. Ambient. ml/min. µl, Pulsed Amperometric Dual Gold Electrode; delay 0 msec E=00mV T=70msec E=000mV T=0msec E=-800mV T=00msec Post Column Conditions: 0.N Sodium Hydroxide at ml/min. I.D. x Length 7 µm -0 µm. x 0 mm x 0 mm x 0 mm x 0 mm x 0 mm* x 0 mm 797. x 0 mm 79 * PEEK hardware Analytical Guard Column Starter Kit ( holder, cartridges) 7976 Replacement Cartridges (/pk) 7977 C8 Guard Column for EPA Glyphosate Application Starter Kit ( holder, cartridges) 799 Replacement Cartridges (/pk) 79 PRP-X00 Semiprep/Preparative Guard Column Starter Kit ( holder, cartridge) 79 Replacement Cartridges (/pk) 79 * Analytical guard column for PEEK columns is.0 x 8.0 m ** Analytical guard column for steel columns is. x 0 mm *** Semiprep/preparative guard column for steel columns is.6 x 0 mm For PEEK Columns* For Steel Columns** For Steel Columns***

23 PRP-X800 Cation Exchange Polymeric cation exchange columns for the isocratic separation of mono and divalent cations. Isocratic separation of lithium, sodium, ammonium, potassium, magnesium and calcium. Gradient separation of transition metals. Excellent durability (stable to any concentration of water or organic solvent). One particle size: 7 µm. Two column internal diameters:. to.6 mm. Two column materials: 6 stainless steel and PEEK. Guard columns in 6 stainless steel and PEEK. Mono and Divalent Cations Transition Metals Transition Metals PRP-X800,. x 0 mm, 7 µm (P/N 7988). Lithium ppm. Sodium ppm. Ammonium ppm. Potassium ppm. Magnesium ppm 6. Calcium ppm 0 6 Conditions: mm Cupric Sulfate. Isocratic. Ambient. 0.8 ml/min. 0 µl, Indirect UV 0 nm. 6 PRP-X800,. x 0 mm, 7µm (P/N 798). Manganese 6 ppm. Cadmium 6 ppm. Zinc 6 ppm. Cobalt 6 ppm Conditions: A) 0mM Ammonium Acetate. ph.; B) Trifluoroacetic Acid in Water ph.. Linear Gradient -% B in 0 min. ml/min. 00 µl, Post Column Reaction: 0.0% -(-pyridylazo)resorcinol in M Ammonium Hydroxide 0. ml/min. Visible 0 nm. PRP-X800,. x 0 mm, 7µm (P/N 798). Manganese 6 ppm. Zinc 6 ppm. Cadmium 6 ppm. Lead 6 ppm Conditions: A). mm Ethylenediamine ph.; B) 00mM Ethylenediamine ph.7. Linear Gradient 00% A (0- min.), 0-00% B in (-0 min.) ml/min, 00 µl, Post Column Reaction: 0.0% -(-pyridylazo) resorcinol (PAR) in M Ammonium Hydroxide, 0. ml/min. Visible 0 nm. I.D. x Length 7 µm. x 0 mm 798. x 0 mm x 0 mm* x 0 mm* 7989 * PEEK hardware Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated.

24 HC-0 Cation Exchange Polymeric % cross-linked soft-gel column for cation, ligand exchange separation of carbohydrates. Separate oligosaccharides up to DP8. Water mobile phase. Compatible with RI detectors. One particle size: 0 - µm. One column internal diameter: 7.8 mm. One column material: 6 stainless steel. Corn Syrup HC-0, 7.8 x 0 mm, (P/N 79) 76. Glucose. Maltose. Maltotriose. Maltotetraose. Maltopentaose 6. Maltohexaose 7. Maltooctaose + High Conversion Corn Syrup HC-0, 7.8 x 0 mm, (P/N 79). Glucose. Maltose. Maltotriose. Maltotetraose. Maltopentaose 6. Maltohexaose 7. Maltoheptaose Conditions: Water. Isocratic. 80 C. 0.6 ml/min. µl, Refractive Index Conditions: Deionized Water. Isocratic. 80 C. 0.6 ml/min. 0 µl, Refractive Index. Guard Columns For Steel Columns* Gel-Type Cation Exchange Column I.D. x Length 0- µm 7.8 x 0 mm 79 Hydrogen Form Cation Exchange Starter Kit ( holder, cartridge) 79 Hydrogen Form Cation Exchange Replacement Cartridges (/pk) 79 Carbonate Form Anion Exchange Starter Kit ( holder, cartridge) Carbonate Form Anion Exchange Replacement Cartridges (/pk) 7986 * HC-0 guard column for steel columns is.6 x 0 mm

25 HC-7 Cation Exchange Polymeric 7.% cross-linked soft-gel columns for cation, ligand exchange separations of carbohydrates. Compatible with UV and RI detectors. Hydrogen form columns for USP L7 applications. Calcium form columns for USP L9 applications. Lead form columns for USP L applications. One particle size: 9 µm. Three column internal diameters:., 7.8 and 0.0 mm. One column material: 6 stainless steel. Three forms: calcium, lead and hydrogen. Malic Acid Citric, Lactic and Acetic Acids Dihydroxyacetone HC-7,. x 0 mm H + (P/N 7976). Maleic Acid ppm. Malic Acid 000 ppm. Fumaric Acid 0 ppm HC-7,. x 0 mm H + (P/N 7976). Citric, Acid 0 ppm. Lactic Acid 0 ppm. Acetic Acid 0 ppm HC-7, 7.8 x 0 mm Ca + (P/N 796). Formic Acid 0. mg/ml. Glycerol 0. mg/ml. Dihydroxyacetone 0. mg/ml 0 0 Conditions: 0.0N Sulfuric Acid. Isocratic. 60 C. 0. ml/min. 0 µl, UV 0 nm. 0 6 Conditions: 0.0N Sulfuric Acid. Isocratic. 60 C. 0. ml/min. 0 µl, UV 0 nm. 0 0 Conditions: 0.0M Calcium Chloride. Isocratic. 90 C. 0.6 ml/min. ul, Refractive Index. Gel-Type Cation Exchange Columns 9 µm, 9 µm, 9 µm, I.D. x Length Calcium Lead Hydrogen. x 0 mm x 00 mm x 0 mm x 0 mm 798 Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated. Guard Columns Hydrogen Form Cation Exchange Starter Kit ( holder, cartridge) 79 Hydrogen Form Cation Exchange Replacement Cartridges (/pk) 79 Carbonate Form Anion Exchange Starter Kit ( holder, cartridge) Carbonate Form Anion Exchange Replacement Cartridges (/pk) 7986 * HC-7 guard column for steel columns is.6 x 0 mm For Steel Columns*

26 Ion Exclusion PRP-X00 Polymeric ion exclusion packing for separation of alcohols and organic acids. Separate structurally similar alcohols. Separate similar organic acids. Use with organic solvent from 0 to 00% for elution of highly retained compounds. One particle size: 7 µm. Two column materials: 6 stainless steel and PEEK. Five column internal diameters:.0 to 0.0 mm. Analytical and semiprep/preparative guard columns. Fatty Acids Acrylamide and Acrylic Acid Organic Acids PRP-X00,. x 0 mm (P/N 796). Acetic Acid 00 ppm. Propionic Acid 00 ppm. Isobutyric Acid 00 ppm. Butyric Acid 00 ppm. Isovaleric Acid 00 ppm 6. Valeric Acid 00 ppm 6 PRP-X00,. x 0 mm (P/N 796). Acrylamide ppm. Acrylic Acid 0 ppm. Acrylonitrile 67 ppm PRP-X00,. x 0 mm (P/N 796). Tartaric Acid. Malic Acid. Citric Acid. Lactic Acid. Acetic Acid Conditions: 0:80 t-butyl Alcohol : 0mM Potassium Phosphate Monobasic ph.. Isocratic. Ambient. ml/min. 00 µl, UV nm. 0 6 Conditions: 9: mn Sulfuric Acid : Acetonitrile. Isocratic. Ambient. ml/min. µl, UV 0 nm. 0 6 Conditions: mn Sulfuric Acid. Isocratic. Ambient. ml/min. 0 ul, UV 0 nm. I.D. x Length 7 µm.0 x 0 mm x 00 mm x 0 mm x 0 mm x 0 mm x 0 mm x mm 796. x 0 mm 796. x 0 mm 796. x 0 mm x 0 mm* x 0 mm* x 0 mm 797 * PEEK hardware Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm Semiprep/Preparative Guard Column Starter Kit ( holder, cartridge) 799 Replacement Cartridges (/pk) 790 * Semiprep/preparative guard column for steel columns is.6 x 0 mm Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com For Steel Columns*