HPLC. Columns and Resins. For a Complete Range of Applications

Transcription

1 HPLC Columns and Resins For a Complete Range of Applications

2 0 HPLC COLUMNS AND RESINS HAMILTON Polymeric and Silica-Based Packings for a Complete Range of HPLC Separations To order products from this catalogue, please contact the dealer for your country. Other Product Lines Made by HAMILTON: - Syringes - SoftGrip Pipettes - Electrochemical Sensors - Diluters/Dispensers - Valves - OEM Standard Components - Pipetting Robots - Automated Analysers - OEM Components for Automated Liquid Handling

3 HPLC COLUMNS AND RESINS 0 Table of Contents Introduction Reversed Phase Columns PRP- PRP- PRP-Infinity HxSil C8, HxSil C8 Anion Exchange Columns PRP-X00 PRP-X0(S) PRP-X00 PRP-X600 RCX-0 RCX-0 Cation Exchange Columns PRP-X00 PRP-X00 PRP-X800 HC-0 HC-7 Ion Exclusion PRP-X00 Amino Columns PRP-X700 Bulk Resins

4 0 HPLC COLUMNS AND RESINS Introduction A Word About HPLC Columns HAMILTON is one of the few companies that manufactures its own HPLC grade polymeric HPLC supports. We offer 6 polymeric packings for a complete range of HPLC applications, including reversed phase, anion exchange, cation exchange, ion exclusion and amino. For over 0 years HAMILTON polymeric HPLC columns have been solving problems no other columns could. Now we have introduced HxSil silica-based reversed phase C8 and C8 HPLC columns to complement our selection of polymer-based columns. At HAMILTON we believe that your separation requirements dictate the type of column needed. Whether it's a silica-based or polymerbased column we are ready to help. Contact us at hplc@hamilton.ch for expert technical assistance and FREE method development. HPLC Column Selection Made Easy! This brochure is organised so you can easily locate the product of interest. Columns are grouped by the separation mechanism (reversed phase, anion exchange, cation exchange, ion exclusion and amino). A few chromatograms are included to illustrate possible separations. A complete listing of HAMILTON HPLC applications and technical information can be found on the HAMILTON web site under Analytical, HPLC Columns and Resins at Longer Column Life HAMILTON is a pioneer in the development and manufacture of polymers for HPLC separations. These polymeric supports combine the inertness, durability and ph stability of polymeric resins with the pressure stability of silica-based materials. With HAMILTON polymeric HPLC columns you let your sample dictate the necessary separation conditions not the column. ph Stability Our polymeric HPLC columns are stable from ph 0 to. This allows the separation of ionic samples in their natural state where UV absorbance is typically stronger and the minimum level of detectability is improved. Additionally samples that are only stable or soluble at elevated ph can be separated. Better Sample Recovery HAMILTON polymeric HPLC columns do not contain acidic silanol groups as present on silica supports. This eliminates irreversible sample adsorption to the support, greatly improves sample recovery (greater than 9 % recovery of purified trityl-on DNA). Excellent Durability HAMILTON polymeric HPLC columns are compatible with 0 to 00 % organic or aqueous solvents, do not shrink or swell in different mobile phases, do not bleed bonded phases like silica-based supports. Consistent Performance We manufacture our polymeric supports from the respective monomer precursors. In doing so, we are able to control every aspect of the polymer's synthesis, sizing and eventual packing. With this control, we are able to ensure reproducible resin performance, batch after batch. Also HAMILTON's new HxSil silica-based reversed phase C8 and C8 HPLC columns are manufactured to provide reproducible retention of your compound. This is guaranteed by tight manufacturing controls and extensive characterisation of the base silica.

5 HPLC COLUMNS AND RESINS 0 Introduction HxSil Silica-Based Columns HAMILTON's new HxSil silica-based reversed phase C8 and C8 HPLC columns provide greater retention of compounds than other C8 and C8 columns. This allows you to separate compounds that are not adequately retained on other reversed phase columns. When you combine HxSil columns with our selection of polymer-based reversed phase columns you have a wide range of column retention selectivity and performance characteristics from which to choose. HPLC Column Packings A brief description of the HPLC columns is contained below. All column types except HxSil C8 and HxSil C8 are of polymeric nature. Visit the HAMILTON Application Index at myappindex.html or hplc@hamilton.ch for a complete list of compounds that have been separated. Recommended Uses Reversed Phase Anion Exchange Cation Exchange Ion Exclusion Amino Phase Bulk Resins PRP- PRP- PRP-Infinity HxSil C8, HxSil C8 PRP-X00 PRP-X0(S) PRP-X00 PRP-X600 RCX-0 RCX-0 PRP-X00 PRP-X00 PRP-X800 HC-0 HC-7 Calcium Form HC-7 Hydrogen Form HC-7 Lead Form PRP-X00 PRP-X700 All HPLC column packings listed above Broad range of uses, similar to a C8, C8 column. Gradient protein and peptide separations. Fast gradient separations of large proteins and peptides. General purpose silica-based reversed phase columns. Inorganic anions such as fluoride, chloride, bromide through sulfate from 0 to 00 ppm. Inorganic anions in waste water and drinking water from 0 ppb to 0 ppm. Designed for EPA method #00.0 Part A. Gradient protein separations on a limited porosity support. DNA oligomer separations on a limited porosity support. Sugar separations up to DP at high ph. Better separation of complex sugars at high ph. Inorganic monovalent or divalent cations from 0 ppb to 00 ppm. Glyphosate and its metabolite in drinking water. Inorganic monovalent and divalent cations including transition metals. Low pressure (<,000 psi) separation of sugar oligomers up to DP8. Low pressure (<,000 psi) separation of mono and disaccharides in corn syrup. Low pressure (<,000 psi) separation of organic acids and sugars. Low pressure (<,000 psi) separation of sugar alcohols. Organic acid and alcohol separations in fruits and beverages. Normal phase separation of sugars up to DP8 in isocratic or gradient mode. Preparative/process scale purifications.

6 06 HPLC COLUMNS AND RESINS Introduction USP "L" Number and their HPLC Column Description USP "L" Number HAMILTON Column L L7 L7 L9 L L L Octadecylsilane chemically bonded to porous silica or ceramic microparticles, to 0 µm in diameter. Octylsilane chemically bonded to porous silica particles, to 0 µm in diameter. Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to µm in diameter. Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, about 9 µm in diameter. A rigid, spherical styrene-divinylbenzene copolymer, to 0 µm in diameter. An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, about 0 µm in size. Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead form, about 9 µm in diameter. HxSil C8 HxSil C8 HC-7 Hydrogen Form HC-7 Calcium Form HC-0 PRP- PRP- PRP-Infinity PRP-X00 HC-7 Lead Form

7 Reversed Phase HPLC COLUMNS AND RESINS 07 Reversed Phase HPLC Columns HPLC Columns PRP- PRP-, polymeric 00 Å columns for general reversed phase separations. ph stable from 0 to. Better sample recovery than silica-based columns (there are no silanol groups). Five particle sizes:,, 7, 0 and - 0 µm. Eleven column internal diameters:.0 to 0.6 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. Excellent durability (stable to any concentration of water or organic solvent). What Types of Samples Can PRP- Columns Separate?... Antibiotics Chiral DNA Oxytetracycline and Tetracycline PRP-,. x 0 mm. Oxytetracycline 60 ppm. Tetracycline 0 ppm d and l Malic and Tartaric Acid PRP-,. x 0 mm. l-malic Acid, 00 ppm. d-malic Acid, 00 ppm. l-tartaric Acid, 00 ppm. d-tartaric Acid, 00 ppm Fluorescein-Labelled Oligonucleotide mer PRP-,. x 0 mm. Failure Sequences. Fluorescein-Labelled mer min. Conditions: 0 gm t-butyl Alcohol, 60 ml 0. M Phosphate buffer (ph 7.), 0 ml % Tetrabutylammonium Hydrogen Sulfate, 0 ml 0.0 % EDTA diluted to L with DI Water. Isocratic. 60 C. ml/min. 00 µl. UV at nm. 0 6 min. Conditions: 8 mm Copper (II) Acetate, 6 mm l-valine, ph.. Isocratic. Ambient ml/min. UV at 0 nm. 0 6 min. Conditions: A) 0 mm Sodium Acetate ph 6.7, B) Acetonitrile. Linear Gradient 0-0 % B in 0 min. Ambient. UV at 60 nm.

8 08 HPLC COLUMNS AND RESINS Reversed Phase HPLC Columns DNA Herbicides Pharmaceuticals PRP-,. x 0 mm. &. Failure Sequences. -DMT-0mer Diquat and Paraquat PRP-,. x 0 mm. Diquat, 000 ppm. Paraquat, 000 ppm PRP- Cartridge Column,. x mm. Morphine, 0 ug/ml. Codeine, 0 ug/ml. Thebaine, 0 ug/ml. Methadone, 0 ug/ml 0 0 min. Conditions: A) 0. M Triethylammonium Acetate ph 7.; B) Acetonitrile. Linear Gradient 0-0 % B (0-8 min.), 0-80 % B (8 - min.). Ambient. ml/min. UV 60 nm. 0. min. Conditions: 0. N Phosphoric Acid, 0.0 M Diethylamine, 0.06 M -Hexanesulfonic Acid Sodium Salt. C..0 ml/min. UV 9 nm. 0 6 min. Conditions: A) 0 mm Ammonium Hydroxide; B) Acetonitrile. Linear Gradient 0-8 % (0-0 min), hold 8 % B for min. Ambient. 0. ml/min. UV 0 nm. PRP- Ordering Information PRP-, 00 Å Reversed Phase HPLC Columns I.D. x Length µm µm 7 µm 0 µm -0 µm.0 x 0 mm R797 R797 R x 00 mm R797 R7976 R x 0 mm R7987 R797 R7977 R x 0 mm R797 R7978 R7976. x 0 mm R798 R79. x 00 mm R798 R x 0 mm R798 R7966 R7980. x 0 mm* R x 0 mm R7990 R799. x mm R79789**. x 0 mm R7980 R79. x 00 mm R7980 R7979 R796. x 0 mm R79806 R79 R799 R79 R797. x 0 mm R7980 R79 R797 R798.6 x 0 mm* R7980 R x 00 mm* R798.6 x 0 mm* R79 R79 I.D. x Length µm µm 7 µm 0 µm -0 µm.6 x 0 mm* R797 R7980 R x 00 mm R799 R x 00 mm R x 0 mm R7979 R79 R x 0 mm R x 00 mm R79 R x 0 mm R x 0 mm R79 R7996. x 7 mm. x 00 mm R7979. x 0 mm R79 R7997. x 0 mm R79 R7978 R x 00 mm R x 00 mm R x 0 mm R x 0 mm R7967 R x 0 mm R79 * PEEK hardware ** Cartridge Column, Cartridge Holder P/N R908 must be purchased separately. PRP- Analytical Guard Columns For Steel Columns* For PEEK Columns** PRP- Semiprep/Preparative Guard Columns For Steel Columns*** Starter Kits R797 R797 Starter Kits R79 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) R79 R798 Replacement Cartridges (/pk) R79 * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm *** Semiprep/preparative guard column for steel columns is.6 x 0 mm Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

9 HPLC COLUMNS AND RESINS 09 Reversed Phase HPLC Columns PRP- PRP-, polymeric 00 Å columns for protein separations. ph stable from 0 to. Better sample recovery than silica-based columns (there are no silanol groups). Excellent durability (the support can be cleaned with strong acid or base to remove any sample residue). Three particle sizes:, 0 and - 0 µm. Eight column internal diameters:.0 to 0.8 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. What Types of Samples Can PRP- Columns Separate?... Peptides Proteins Proteins Tryptic Digest of Cytochrome c PRP-,. x 0 mm PRP-,. x 0 mm. Ribonuclease A. Cytochrome c. Lysozyme. Myoglobin. Ovalbumin Proteins at ph. PRP-,. x 0 mm 6. Ribonuclease A. Insulin. Cytochrome c. Trypsin. Lysozyme 6. Pyruvate Dehydrogenase min. Conditions: A) 0. % TFA in Water ph.0; B) 0. % TFA in Acetonitrile. Linear Gradient - 60 % B in 0 min. Ambient. 0. ml/min. UV nm. 0 8 min. Conditions: A) 0. % TFA in Water ph.0; B) 0. % A in Acetonitrile. Linear Gradient - 0 % B in min. Hold min. Ambient. ml/min. UV nm. 0 0 min. Conditions: A) 0. % TFA in 0 mm Sodium Hydroxide ph.; B) 0. % TFA in Acetonitrile. Linear Gradient 0-60 % B in 0 min. Ambient. ml/min. UV 0 nm. PRP- Ordering Information PRP-, 00 Å Reversed Phase HPLC Columns I.D. x Length µm 0 µm - 0 µm.0 x 0 mm R x 00 mm R x 0 mm R x 0 mm R x 0 mm R7988. x 00 mm R7986. x 0 mm R7986 R799. x 0 mm R79807 R7967. x 00 mm R79808 I.D. x Length µm 0 µm - 0 µm. x 0 mm R79809 R7966. x 0 mm R x 0 mm* R799.6 x 0 mm* R798.6 x 0 mm* R x 0 mm R x 0 mm R796. x 0 mm R x 0 mm R7987 * PEEK hardware PRP- Analytical Guard Columns For Steel Columns* For PEEK Columns** PRP- Semiprep/Preparative Guard Columns For Steel Columns* Starter Kits R796 R799 Starter Kits R79 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) R79 R799 Replacement Cartridges (/pk) R79 * Analytical guard column for steel columns is. x 0 mm * Semiprep/preparative guard column for steel columns is.6 x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm

10 0 HPLC COLUMNS AND RESINS Reversed Phase HPLC Columns PRP-Infinity PRP-Infinity, polymeric nonporous packing for fast gradient separation of large proteins. ph stable from 0 to. Better sample recovery than silica-based columns (there are no silanol groups). Excellent durability (the support can be cleaned with strong acid or base to remove any sample residue). One particle size: µm. Three column internal diameters:. to 0.0 mm. One column material: 6 stainless steel. What Types of Samples Can PRP-Infinity Columns Separate?... Proteins Proteins Proteins Nine Proteins PRP-Infinity,. x 0 mm. Ribonuclease A. Insulin 6. Cytochrome c. Lysozyme Transferrin 6. BSA 7. Myoglobin 8. Concanavalin A 9. Ovalbumin Protein Standards PRP-Infinity,. x 0 mm 6. Ribonuclease A. Cytochrome c. Transferrin. BSA. Concanavalin A 6. Ovalbumin Egg White Proteins PRP-Infinity,. x 0 mm. Conalbumin. Ovalbumin 0 min. Conditions: A) 0. % TFA in Water ph.0; B) 0. % TFA in Acetonitrile. Linear Gradient 0-60 % B in min. Ambient. ml/min. UV nm. 0 0 min. Conditions: A) 0. % TFA in Water ph.0; B) 0. % TFA in Acetonitrile. Linear Gradient 0-60 % B in 0 min. Ambient. ml/min. UV nm. 0. min. Conditions: A) 0. % TFA in Water; B) 0. % TFA in Acetonitrile. Linear Gradient 0-60 % B in 0 min. Ambient. ml/min. UV nm. PRP-Infinity Ordering Information PRP-Infinity, Non-porous Reversed Phase HPLC Columns I.D. x Length µm. x 0 mm R7976. x 00 mm R7978. x 0 mm R7970. x 0 mm R x 60 mm R797 For PRP-Infinity columns an in-line filter is recommended as a guard column is not available. Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

11 HPLC COLUMNS AND RESINS Reversed Phase HPLC Columns HxSil C8 & HxSil C8 HxSil C8 and HxSil C8, silica-based 00 Å columns for general reversed phase separations. Separation of closely related PAHs. Better retention of poorly retained compounds. Two particle sizes: and µm. Five column internal diameters:. to. mm. Separation of neutral, acidic and basic compounds without tailing (tailing factor of less than.0). Analytical and semiprep/preparative guard columns in 6 stainless steel. What Types of Samples Can HxSil Columns Separate?... PAHs Sunscreen Compounds Steroids Polycyclic Aromatic Hydrocarbons HxSil C8,.6 x 0 mm min.. Benzene. Naphthalene. Acenaphthylene. Acenaphthene. Phenanthrene 6. Anthracene 7. Pyrene 8. Chrysene 9. Benzo[b]fluoranthene 0. Benzo[k]fluoranthene.,,,6-Dibenzanthracene Conditions: 7: Acetonitrile : Water. Isocratic. Ambient. ml/min. UV nm min. Sunscreen Compounds in Lip Balm HxSil C8,.6 x 0 mm. Oxybezone. Octocrylene. Octyl Methoxycinnamate. Avobenzone (Parsol 789). Octyl Salicylate Conditions: 8: Methanol : Water. Isocratic. Ambient. ml/min. UV 08 nm. 0 6 min. Steroids HxSil C8,.6 x 0 mm. Hydrocortisone. -Androstene-, 7-dione. Testosterone. Progesterone Conditions: 8: Methanol : Water. Isocratic. Ambient. ml/min. UV 0 nm. HxSil Ordering Information HxSil C8, 00 Å Reversed Phase HPLC Columns I.D. x Length µm µm. x 0 mm R796. x 0 mm R797 R7907. x 7 mm R798 R7908. x 00 mm R799 R7909. x 0 mm R790 R790. x 0 mm R79.6 x 0 mm R79 I.D. x Length µm µm.6 x 0 mm R79 R x 00 mm R79 R790.6 x 0 mm R79 R790.6 x 0 mm R x 0 mm R x 0 mm R790. x 0 mm R7906 HxSil C8 Analytical Guard Columns For Steel Columns* HxSil C8 Semiprep/Preparative Guard Columns For Steel Columns* Starter Kits R798 Starter Kits R79 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) R79 Replacement Cartridges (/pk) R796 * Analytical guard column for steel columns is. x 0 mm * Semiprep/preparative guard column for steel columns is.6 x 0 mm

12 HPLC COLUMNS AND RESINS Reversed Phase HPLC Columns HxSil C8, 00 Å Reversed Phase HPLC Columns I.D. x Length µm µm. x 0 mm R x 0 mm R79889 R7988. x 7 mm R79890 R7988. x 00 mm R7989 R7988. x 0 mm R7989 R7988. x 0 mm R x 0 mm R79886 I.D. x Length µm µm.6 x 0 mm R7987 R x 00 mm R79887 R x 0 mm R7987 R x 0 mm R x 0 mm R x 0 mm R x 0 mm R7987 HxSil C8 Analytical Guard Columns For Steel Columns* HxSil C8 Semiprep/Preparative Guard Columns For Steel Columns* Starter Kits R799 Starter Kits R797 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) R79 Replacement Cartridges (/pk) R798 * Analytical guard column for steel columns is. x 0 mm * Semiprep/preparative guard column for steel columns is.6 x 0 mm Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

13 Anion Exchange HPLC COLUMNS AND RESINS Anion Exchange HPLC Columns HPLC Columns PRP-X00 PRP-X00 polymeric anion exchange packings for separation of inorganic and organic anions from 0 to 00 ppm. Easily separate the eight common anions (fluoride through sulfate). Good separation of fluoride from the water dip. Five particle sizes:,, 7, 0 and - 0 µm. Eight column internal diameters:.0 to 0.8 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. Use with organic solvent from 0 to 00 % for elution of hydrophobic anions or column cleaning. ph stable from 0 to. Use conductivity or UV detection. What Types of Samples Can PRP-X00 Columns Separate?... Anticancer Agents Common Anions Common Anions Glucaric Acid PRP-X00,. x 0 mm. Glucaric Acid Eight Anions by Conductivity PRP-X00,. x 0 mm. Fluoride, 0 ppm. Chloride, 0 ppm. Carbonate, 0 ppm. Nitrite, 0 ppm. Bromide, 0 ppm 6. Nitrate, 0 ppm 7. Phosphate, 0 ppm 8. Sufate, 0 ppm Phosphate, Phosphite and Other Anions PRP-X00,. x 0 mm. Fluoride, 0 ppm. Phosphate, 0 ppm. Phosphite, 0 ppm. Chloride, 0 ppm 0 8 min. Conditions: : Acetonitrile : 0 mm Potassium Phosphate ph 6.. Isocratic. Ambient. ml/min. UV 0 nm. 0 0 min. Conditions: mm p-hydroxybenzoic Acid ph 8.9 with. % Methanol. Isocratic. Ambient. ml/min. Conductivity min. Conditions: 0 mm Succinic Acid, ph.9. Isocratic. Ambient. ml/min. Conductivity.

14 HPLC COLUMNS AND RESINS Anion Exchange HPLC Columns Common Anions Nuclear Fuel Processing Organic Acids Iodide and Anion Standards PRP-X00,. x 0 mm. Fluoride, 0 ppm. Chloride, 0 ppm. Nitrite, 0 ppm. Phosphate, 0 ppm. Bromide, 0 ppm 6. Nitrate, 0 ppm 7. Sulfate, 0 ppm 8. Iodide, 0 ppm Conditions: 7 8 mm -Amino--hydroxybenzoic Acid ph Isocratic. Ambient. ml/min. Conductivity min. 0. min. Butylphosphates PRP-X00,. x 0 mm. Dibutylphosphate. Monobutylphosphate Conditions: : 8 mm Trisodium Phosphate : Acetonitrile. Isocratic. Ambient ml/min. UV 0 nm. Acetate and Chloride PRP-X00,. x 0 mm. Acetate, 00 ppm. Chloride, 0 ppm 0 8 min. Conditions: 9: 0 mm Potassium Hydrogen Phthalate ph. : Acetonitrile. Isocratic. Ambient. ml/min. Indirect UV 80 nm. OTC Products Supplements Water Disinfection Fluoride in Toothpaste PRP-X00. x 00 mm. Fluoride. Phosphate Taurine in Complex Vitamin Sample PRP-X00,. x 0 mm. Taurine Bromate and Other Anions PRP-X00,. x 0 mm. Fluoride, 0 ppm. Chloride, 0 ppm. Bromate, 0 ppm. Bromide, 0 ppm. Nitrate, 0 ppm 0 8 min. Conditions: 0 mm Succinic Acid, ph.9. Isocratic. Ambient. ml/min. Conductivity. 0 8 min. Conditions: 0 mn Sodium Hydroxide. Isocratic. Ambient. ml/min. Indirect Conductivity. 0 8 min. Conditions:.7 mm Sodium Bicarbonate,.8 mm Sodium Carbonate, 0. mm Sodium Thiocyanate. Isocratic. Ambient. ml/min. Suppressed Conductivity. PRP-X00 Ordering Information PRP-X00, 00 Å Anion Exchange Columns I.D. x Length µm µm 7 µm 0 µm -0 µm.0 x 0 mm R79767 R x 00 mm R79768 R x 0 mm R79878 R79769 R x 0 mm R79770 R7977. x 0 mm R790 R7979. x 0 mm R79. x 0 mm* R798 R798. x 0 mm R796. x 0 mm R7980 R796. x 00 mm R798 R798 R799. x mm R796. x 0 mm R798 R79 I.D. x Length µm µm 7 µm 0 µm -0 µm. x 0 mm R79 R799.6 x 0 mm* R79.6 x 0 mm* R x 0 mm R x 0 mm R x 0 mm R79. x 00 mm R79. x 0 mm R79. x 0 mm R79 R x 0 mm R x 0 mm R79876 R790 PRP-X00 Analytical Guard Columns For Steel Columns** For PEEK Columns*** PRP-X00 Semiprep/Preparative Guard Columns For Steel Columns**** Starter Kits R798 R798 Starter Kits R79 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) R796 R798 Replacement Cartridges (/pk) R796 * PEEK hardware ** Analytical guard column for steel columns is. x 0 mm *** Analytical guard column for PEEK columns is.0 x 8.0 mm **** Semiprep/preparative guard column for steel columns is.6 x 0 mm

15 HPLC COLUMNS AND RESINS Anion Exchange HPLC Columns PRP-X0 & PRP-X0S PRP-X0 and PRP-X0S polymeric anion exchange packings for separation of inorganic and organic anions from 0 ppb to 0 ppm. Easily separate the anions in EPA method #00.0 Part A. Good separation of fluoride from the water dip. Use with organic solvent up to 00 % for elution of Two particle sizes: and 7 µm. Four column internal diameters:.0 to.6 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. hydrophobic anions or column cleaning. ph stable from 0 to. PRP-X0 for non-suppressed ion chromatography and PRP-X0S columns for chemically suppressed ion chromatography. Compatible with conductivity or UV detectors. What Types of Samples Can PRP-X0 & PRP-X0S Columns Separate?... Common Anions Common Anions Common Anions PRP-X0S,. x 0 mm PRP-X0,. x 0 mm PRP-X0S,. x 0 mm. Fluoride, ppm. Chloride, ppm. Nitrite, ppm. Bromide, ppm. Nitrate, ppm 6. Phosphate, ppm 7. Sulfate, ppm 6 7. Fluoride, ppm. Chloride, ppm. Nitrite, ppm. Bromide, ppm. Nitrate, ppm 6. Phosphate, ppm 7. Sulfate, ppm 6 7. Fluoride, 0 ppb. Chloride, 0 ppb. Nitrite, 0 ppb. Bromide, 0 ppb. Nitrate, 0 ppb 6. Phosphate, 0 ppb 7. Sulfate, 0 ppb min. Conditions:.7 mm Sodium Bicarbonate,.8 mm Sodium Carbonate, 0. mm Sodium Thiocyanate. Isocratic. Ambient. ml/min. Suppressed Conductivity. 0 6 min. Conditions:.0 mm p-hydroxybenzoic Acid ph 9.. Isocratic. Ambient. ml/min. Non-Suppressed Conductivity. 0 0 min. Conditions:.7 mm Sodium Bicarbonate,.8 mm Sodium Carbonate. 0. mm Sodium Thiocyanate. Isocratic. Ambient. ml/min. Suppressed Conductivity. High ph Anions Anions EPA Method #00.0 Borate, Silicate and Fluoride PRP-X0,. x 0 mm. Borate, ppm. Silicate, ppm. Fluoride, ppm Cyanide, Chloride and Nitrate PRP-X0,. x 0 mm. Chloride, ppm. Cyanide, ppm. Nitrite, ppm PRP-X0S,. x 0 mm. Fluoride, ppm. Chloride, ppm. Nitrite, ppm. Bromide, ppm. Nitrate, ppm 6. Phosphate, ppm 7. Sulfate, ppm min. Conditions: 0. mm Sodium Hydroxide, 0. mm Sodium Benzoate. Isocratic. Ambient. ml/min. Conductivity. 0 6 min. 0 6 min. Conditions: 0 mm Sodium Hydroxide, 0. mm Sodium Acetate. Isocratic. Ambient.. ml/min. Conductivity. Conditions:.7 mm Sodium Bicarbonate,.8 mm Sodium Carbonate. 0. mm Sodium Thiocyanate. Isocratic. Ambient. ml/min. Suppressed Conductivity.

16 6 HPLC COLUMNS AND RESINS Anion Exchange HPLC Columns PRP-X0 & PRP-X0S Ordering Information PRP-X0, 00 Å Anion Exchange Columns PRP-X0 PRP-X0 PRP-X0S I.D. x Length µm 7 µm 7 µm.0 x 0 mm R x 00 mm R x 0 mm R x 0 mm R x 00 mm R797 R797. x 0 mm R797 R797. x 0 mm R7976 R7977. x 00 mm R798 R7970 R797. x 0 mm R798 R797 R797. x 0 mm* R797 R797.6 x 00 mm* R7976 R x 0 mm* R7978 R x 0 mm* R7970 R797 PRP-X0 Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits R7976 R7977 ( holder, cartridges) Replacement Cartridges (/pk) R7978 R7979 * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated. * PEEK hardware

17 HPLC COLUMNS AND RESINS 7 Anion Exchange HPLC Columns PRP-X00 PRP-X00 polymeric strong-base anion exchange packing with limited porosity for separation of proteins. Fast separation of large proteins. ph stable from 0 to 9. Good recovery without sample ghosting. Higher sample capacity than nonporous supports. One particle size: 7 µm. Three column internal diameters:. to 0.0 mm. Two column materials: 6 stainless steel and PEEK. Guard column in PEEK. What Types of Samples Can PRP-X00 Columns Separate?... Proteins Proteins Proteins PRP-X00.6 x 0 mm. Myoglobin, 7 ug. Conalbumin, 7 ug. Dog Albumin, 77 ug PRP-X00.6 x 0 mm. Myoglobin, 0. mg. Conalbumiin, 0. mg. Ovalbumin, 0. mg. BSA, 0. mg Soybean Trypsin Inhibitor PRP-X00.6 x 0 mm. Soybean Trypsin Inhibitor 0. min. Conditions: A) 0 mm Tris ph 9.0; B) 0 mm Tris ph 9.0, 0. N Sodium Chloride. Linear Gradient 0-0 % B in. min. Ambient. ml/min. UV nm. 0. min. 0 6 min. Conditions: A) 0 mm Tris ph 8.0; B) 0 mm Tris ph 8.0, 0. N Sodium Chloride. Linear Gradient 0-00 % B in. min. Ambient. ml/min. UV 80 nm. Conditions: A) 0 mm Tris ph 9.0; B) 0 mm Tris ph 9.0, 0. N Sodium Chloride. Linear Gradient 0-0 % B in. min. Hold for 0. min. Ambient. ml/min. UV nm. PRP-X00 Ordering Information PRP-X00, Superficially Porous Anion Exchange HPLC Columns PRP-X00 Analytical Guard Columns For PEEK Columns* I.D. x Length 7 µm. x 0 mm R7986. x 0 mm R79.6 x 0 mm* R797.6 x 0 mm* R x 00 mm R798 Starter Kits ( holder, cartridges) R799 Replacement Cartridges (/pk) R790 * Analytical guard column for PEEK columns is.0 x 8.0 mm * PEEK hardware Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

18 8 HPLC COLUMNS AND RESINS Anion Exchange HPLC Columns PRP-X600 PRP-X600 polymeric weak-base anion exchange packing for gradient separation of proteins and DNA oligomers. Limited porosity allows separation of large DNA fragments. ph stable from 0 to. Good recovery without sample ghosting. Higher sample capacity than nonporous supports. One particle size: 7 µm. One column internal diameter:.6 mm. One column material: PEEK. Guard column in PEEK. What Types of Samples Can PRP-X600 Columns Separate?... DNA Oligomers Proteins pbr DNA Fragment PRP-X600,.6 x 0 mm PRP-X600,.6 x 0 mm. Myoglobin, mg/ml. Conalbumiin, mg/ml. Ovalbumin, mg/ml. BSA, mg/ml min. Conditions: A) 0 mm Tris, mm EDTA ph 9.0; B) N NaCl in A. Linear Gradient % B in min % B (- min.). Ambient. ml/min. UV 60 nm. 0 7 min. Conditions: A) 0 mm Tris, ph 9.0; B) 0 mm Tris, ph M NaCl. Linear Gradient 0 - % B (0-0 min.), Hold % B for min. ml/min. UV 0 nm. PRP-X600 Ordering Information PRP-X600, Superficially Porous Anion Exchange HPLC Columns I.D. x Length 7 µm.6 x 0 mm* R7960 PRP-X600 Analytical Guard Columns Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) For PEEK Columns* R796 R796 * PEEK hardware * Analytical guard column for PEEK columns is.0 x 8.0 mm Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

19 HPLC COLUMNS AND RESINS 9 Anion Exchange HPLC Columns RCX-0 RCX-0 polymeric anion exchange packing for isocratic or gradient separation of carbohydrates. ph stable from 0 to. Isocratic separation of mono and disaccharides. Gradient separation of oligomers up to DP8. Compatible with PAD, conductivity and RI detectors. One particle size: 7 µm. Three column internal diameters:. to.6 mm. Two column materials: 6 stainless steel and PEEK. Guard columns in stainless steel and PEEK. What Types of Samples Can RCX-0 Columns Separate?... Artichoke Tubers Trehalose and Glucose Food Carbohydrates RCX-0,. x 0 mm RCX-0,. x 0 mm RCX-0,. x 0 mm. DP. DP. DP0. DP. Trehalose, 000 ppm. Glucose, 000 ppm. Sorbitol, 000 ppm. Glucose, 000 ppm. Fructose, 000 ppm. Sucrose, 000 ppm. Lactose, 000 ppm 6. Maltose, 000 ppm min. Conditions: A) 60 mm Sodium Hydroxide; B) 60 mm Sodium Hydroxide with 00 mm Sodium Acetate. Linear Gradient 0-00 % in 0 min. ml/min. Pulsed Amperometric, Dual Gold Electrode. E = 0 mv E = 800 mv E = -600 mv T = msec T = 66 msec T = 99 msec 0 8 min. Conditions: mn Sodium Hydroxide. Isocratic. Ambient. ml/min. Refractive Index min. Conditions: 0 mn Sodium Hydroxide. Isocratic. Ambient. ml/min. Refractive Index. RCX-0 Ordering Information RCX-0, 00 Å Anion Exchange HPLC Columns I.D. x Length 7 µm. x 0 mm R7999. x 0 mm R790.6 x 0 mm* R7988 RCX-0 Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) R796 R7978 Replacement Cartridges (/pk) R796 R7979 * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm * PEEK hardware Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

20 0 HPLC COLUMNS AND RESINS Anion Exchange HPLC Columns RCX-0 RCX-0 polymeric anion exchange packing for isocratic or gradient separation of complex carbohydrates. ph stable from 0 to. Isocratic or gradient modes. Higher ion exchange capacity support for separation of closely related carbohydrates. Compatible with PAD and RI detectors. One particle size: 7 µm. Two column internal diameters:. to.6 mm. Two column materials: Stainless steel and PEEK. Guard column in PEEK. What Types of Samples Can RCX-0 Columns Separate?... Glycoprotein Monosaccharides Corn Syrup Artichoke Tubers RCX-0,.6 x 0 mm. Fucose. Galactosamine. Glucosamine. Galactose. Glucose 6. Mannose min. Conditions: 0 mn Sodium Hydroxide. Isocratic. Ambient. ml/min. Refractive Index. 6 RCX-0,.6 x 0 mm. Glucose. Fructose RCX-0,.6 x 0 mm. Maltose. Maltotriose. Maltotetraose 6. Maltopentaose 6 7. Maltohexaose Maltoheptaose Maltooctaose 0. Maltononaose. Maltodecaose min. Conditions: A) 60 mm Sodium Hydroxide; B) 00 mm Sodium Acetate in A. Linear Gradient 0-00 % A for min.; then 0-00 % B ( - min.). Ambient. ml/min. Pulsed Amperometric, Dual Gold Electrode. E = 0 mv E = 900 mv E = -80 mv T = 66 msec T = 66 msec T = 00 msec. Glucose. Fructose. Maltose. DP. DP0 6. DP min. Conditions: A) 60 mm Sodium Hydroxide; B) 00 mm Sodium Acetate in A. Linear Gradient 0-0% B in 0 min.; then 0-00 % B (0-0 min.). Ambient. ml/min. Pulsed Amperometric, Dual Gold Electrode. E = 0 mv E = 900 mv E = -800 mv T = 66 msec T = 66 msec T = 00 msec 6 RCX-0 Ordering Information RCX-0, 00 Å Anion Exchange HPLC Columns I.D. x Length 7 µm. x 0 mm R x 0 mm* R x 0 mm* R79877 RCX-0 Analytical Guard Columns For PEEK Columns* Starter Kits ( holder, cartridges) R797 Replacement Cartridges (/pk) R797 * Analytical guard column for PEEK columns is.0 x 8.0 mm * PEEK hardware Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

21 Cation Exchange HPLC COLUMNS AND RESINS Cation Exchange HPLC Columns HPLC Columns PRP-X00 PRP-X00 polymeric cation exchange packing for separation of inorganic cations and organic cations from 0 ppb to 00 ppm. Easily separate mono or divalent cations. ph stable from 0 to 9. One particle size: 0 µm. Six column internal diameters:.0 to 0.0 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. Use with organic solvent up to 00 % for elution of hydrophobic cations and column cleaning. Compatible with conductivity and UV detectors. What Types of Samples Can PRP-X00 Columns Separate?... Monovalent Cations Divalent Cations Neurotransmitters PRP-X00,. x 0 mm PRP-X00,. x 0 mm PRP-X00,. x 0 mm. Lithium, 0 ppb. Sodium, 0 ppb. Ammonium, 0 ppb. Potassium, 0 ppb. Hydrazine, ppm. Magnesium, ppm. Calcium, 0 ppm. Strontium, 0 ppm. Barium, ppm. Choline.0 ug/ml. Acetylcholine.0 ug/ml 0 0 min. Conditions: mm Nitric Acid in 0 % Methanol. Isocratic. Ambient. ml/min. Conductivity. 0 8 min. Conditions:. mm Cupric Sulfate. Isocratic. Ambient. ml/min. Indirect UV at 0 nm min. Conditions: :.0 mn Nitric Acid : Acetonitrile. Isocratic. Ambient. ml/min. Conductivity.

22 HPLC COLUMNS AND RESINS Cation Exchange HPLC Columns PRP-X00 Ordering Information PRP-X00, 00 Å Cation Exchange HPLC Columns I.D. x Length 0 µm I.D. x Length 0 µm.0 x 0 mm R x 0 mm R79.0 x 00 mm R x 0 mm R79.0 x 0 mm R x 0 mm* R798.0 x 0 mm R x 0 mm* R797. x 0 mm R x 0 mm R7989. x 0 mm R x 0 mm R799. x 00 mm R796 * PEEK hardware PRP-X00 Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) R796 R7968 Replacement Cartridges (/pk) R799 R7969 PRP-X00 Semiprep/Preparative Guard Columns For Steel Columns*** Starter Kits ( holder, cartridge) R797 Replacement Cartridges (/pk) R798 * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 m *** Semiprep/preparative guard column for steel columns is.6 x 0 mm Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

23 HPLC COLUMNS AND RESINS Cation Exchange HPLC Columns PRP-X00 PRP-X00 polymeric cation exchange packing for separation of glyphosate and its metabolite. Eight minutes faster analysis time when compared to competitor s columns. Detection levels of less than 0 ppb when using the post column, OPA derivatization technique. Two particle sizes: 7 and - 0 µm. Applicable to other separations, such as ethylene glycol and corn syrup. Five column internal diameters:. to. mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. What Types of Samples Can PRP-X00 Columns Separate?... Glyphosate Ethylene Glycol Corn Syrup PRP-X00,. x 0 mm PRP-X00,. x 0 mm PRP-X00,. x 0 mm. Glyphosate, 00 ppb. Aminomethylphosphonic Acid, 00 ppb. Water. Formaldehyde, 000 ppm. Ethylene Glycol, 000 ppm 6. Fructose, Glucose. Maltose. Maltotriose. Maltotetraose. Maltopentaose 6. Maltohexaose 0 6 min. Conditions: mm Monobasic Potassium Phosphate. Isocratic. Ambient. 0. ml/min. Detection: Ex: 8 nm, Em: nm. Post Column Oxidation and Derivatization. 0. min. Conditions: 80:0 mm Acetonitrile : Water. Isocratic. Ambient. ml/min. Refractive Index min. Conditions: A) Acetonitrile; B) Water. Linear Gradient - % B in 0 min. Ambient. ml/min. Pulsed Amperometric, Dual Gold Electrode. E = 00 mv E = 00 mv E = -800 mv T = 70 msec T = 0 msec T = 00 msec Post Column Conditions: Addition of 0. N Sodium Hydroxide at ml/min. PRP-X00 Ordering Information PRP-X00 Cation Exchange HPLC Columns I.D. x Length 7 µm - 0 µm. x 0 mm R7998. x 0 mm R7989. x 0 mm R7977. x 0 mm R797 R796.6 x 0 mm* R x 0 mm R797. x 0 mm R79 * PEEK hardware PRP-X00 Analytical Guard Columns Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) C8 Guard Columns for EPA Glyphosate Application Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) PRP-X00 Semiprep/Preparative Guard Columns Starter Kits ( holder, cartridge) Replacement Cartridges (/pk) For PEEK Columns* R7976 R7977 For Steel Columns** R799 R79 For Steel Columns*** R79 R79 * Analytical guard column for PEEK columns is.0 x 8.0 m ** Analytical guard column for steel columns is. x 0 mm *** Semiprep/preparative guard column for steel columns is.6 x 0 mm

24 HPLC COLUMNS AND RESINS Cation Exchange HPLC Columns PRP-X800 PRP-X800 polymeric cation exchange columns for the isocratic separation of mono and divalent cations. minute isocratic separation of lithium, sodium, ammonium, potassium, magnesium and calcium. Gradient separation of transition metals. Excellent durability (stable to any concentration of water or organic solvent). One particle size: 7 µm. Two column internal diameters:. to.6 mm. Two column materials: 6 stainless steel and PEEK. Guard columns in 6 stainless steel and PEEK. What Types of Samples Can PRP-X800 Columns Separate?... Mono and Divalent Cations Transition Metals Transition Metals PRP-X800,. x 0 mm PRP-X800,. x 0 mm PRP-X800,. x 0 mm min. 6. Lithium, ppm. Sodium, ppm. Ammonium, ppm. Potassium, ppm. Magnesium, ppm 6. Calcium, ppm Conditions: mm Cupric Sulfate. Isocratic. Ambient. Flow Rate: 0.8 ml/min. Injection: 0 µm Indirect UV 0 nm.. Manganese, 6 ppm. Cadmium, 6 ppm. Zinc, 6 ppm. Cobalt, 6 ppm min. Conditions: A) 0 mm Ammonium Acetate, Adjusted ph to. with Acetic Acid; B) TFA / Water at ph.. Linear Gradient: - % B in 0 min. Flow Rate:.0 ml/min. Injection: 00 µl. Detection: Post Column Reaction: 0.0 % -(- Pyridylazo) Resorcinol (PAR) in M Ammonium Hydroxide at 0. ml/min. Visible 0 nm.. Manganese, 6 ppm. Cadmium, 6 ppm. Zinc, 6 ppm. Lead, 6 ppm min. Conditions: A) mm Ethylenediamine ph. with Acetic Acid; B) 00 mm Ethylenediamine ph.7 with Acetic Acid, TFA. Linear Gradient: 00 % A for min., 0-00 % B in min. Flow Rate: ml/min. Injection: 00 µl. Detection: Post Column Reaction: 0.0 % -(-Pyridylazo) Resorcinol (PAR) in M Ammonium Hydroxide at 0. ml/min. Visible 0 nm. PRP-X800 Ordering Information PRP-X800, 00 Å Cation Exchange HPLC Columns I.D. x Length 7 µm. x 0 mm R798. x 0 mm R x 0 mm* R798.6 x 0 mm* R7989 PRP-X800 Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) R7980 R798 Replacement Cartridges (/pk) R798 R798 * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm * PEEK hardware Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

25 HPLC COLUMNS AND RESINS Cation Exchange HPLC Columns HC-0 HC-0 polymeric % cross-linked soft-gel column for cation, ligand exchange separations of carbohydrates. Separate oligosaccharides up to DP8. Water mobile phase. Compatible with RI detectors. One particle size: 0 - µm. One column internal diameter: 7.8 mm. One column material: 6 stainless steel. What Types of Samples Can HC-0 Columns Separate?... Corn Syrup High-Conversion Corn Syrup HC-0, 7.8 x 0 mm. Glucose. Maltose. Maltotriose. Maltotetraose. Maltopentaose 6. Maltohexaose 7. Maltooctaose + 76 HC-0 Calcium Form, 7.8 x 0 mm. Glucose. Maltose. Maltotriose. Maltotetraose. Maltopentaose 6. Maltohexaose 7. Maltoheptose 8. Maltooctaose min. Conditions: Water. Isocratic. 80 C. 0.6 ml/min. Refractive Index min. Conditions: Deionized Water. Isocratic. 80 C. 0.6 ml/min. Refractive Index. HC-0 Ordering Information HC-0, Gel-Type Cation Exchange Column I.D. x Length 0 - µm 7.8 x 0 mm R79 HC-0 Guard Columns For Steel Columns* Hydrogen Form Cation Exchange Starter Kits ( holder, cartridge) R79 Hydrogen Form Cation Exchange Replacement Cartridges (/pk) R79 Carbonate Form Anion Exchange Starter Kits ( holder, cartridge) R79866 Carbonate Form Anion Exchange Replacement Cartridges (/pk) R7986 * HC-0 guard column for steel columns is.6 x 0 mm

26 6 HPLC COLUMNS AND RESINS Cation Exchange HPLC Columns HC-7 HC-7 polymeric 7. % cross-linked soft-gel columns for cation, ligand exchange separations of carbohydrates. Water mobile phase. Compatible with RI detectors. Hydrogen form columns for USP L7 applications. Calcium form columns for USP L9 applications. Lead form columns for USP L applications. One particle size: 9 µm. Three column internal diameters:., 7.8 and 0.0 mm. One column material: 6 stainless steel. Three forms: calcium, hydrogen and lead. Hydrogen & carbonate form guard columns. What Types of Samples Can HC-7 Columns Separate?... Corn Syrup Organic Acids and Sugars Sugar Alcohols HC-7 Calcium Form, 7.8 x 0 mm. Maltotetraose +. Maltotriose. Maltose. Glucose. Fructose HC-7 Hydrogen Form,. x 0 mm. Maleic Acid, ppm. Malic Acid, 000 ppm. Fumaric Acid, 0 ppm HC-7 Lead Form, 7.8 x 0 mm. Sucrose,. mg/ml. Glucose,. mg/ml. Fructose,. mg/ml. Inositol,. mg/ml. Sorbitol,. mg/ml 0 min. Conditions: Deionized Water 80 C.Isocratic. Ambient. 0.6 ml/min. Refractive Index. 0 0 min. Conditions: 0.0N Sulfuric Acid. Isocratic. 60 C. 0. ml/min. UV 0 nm min. Conditions: Deionized Water 80 C.Isocratic. Ambient. 0.6 ml/min. Refractive Index. HC-7 Ordering Information HC-7, Gel-Type Cation Exchange Columns 9 µm, 9 µm, 9 µm, I.D. x Length Calcium Lead Hydrogen. x 0 mm R79 R x 00 mm R x 0 mm R796 R798 R x 0 mm R798 Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated. HC-7 Guard Columns For Steel Columns* Hydrogen Form Cation Exchange Starter Kits ( holder, cartridge) R79 Hydrogen Form Cation Exchange Replacement Cartridges (/pk) R79 Carbonate Form Anion Exchange Starter Kits ( holder, cartridge) R79866 Carbonate Form Anion Exchange Replacement Cartridges (/pk) R7986 * HC-7 guard column for steel columns is.6 x 0 mm

27 Ion Exclusion HPLC COLUMNS AND RESINS 7 Ion Exclusion HPLC Columns HPLC Columns PRP-X00 PRP-X00 polymeric ion exclusion packing for separation of similar alcohols and organic acids. Separate structurally similar alcohols. Separate similar organic acids. Use with organic solvent from 0 to 00 % for elution of highly retained compounds. Two particle sizes: and 7 µm. Five column internal diameters:.0 to 0.0 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. What Types of Samples Can PRP-X00 Columns Separate?... Alcohols Organic Acids Organic Acids Nine Alcohol Standards PRP-X00,. x 0 mm 6. Glycerol 6. t-butanol. Methanol 7. s-butanol. Ethanol 8. i-butanol. i-propanol 9. n-butanol. n-propanol Fatty Acids, PRP-X00,. x 0 mm. Acetic Acid, 00 ppm. Propionic Acid, 00 ppm. Isobutyric Acid, 00 ppm. Butyric Acid, 00 ppm. Isovaleric Acid, 00 ppm 6. Valeric Acid, 00 ppm 6 PRP-X00,. x 0 mm. Maleic Acid. Malonic Acid. Succinic Acid. Fumaric Acid. Endothall Acid 0. min. Conditions: 9: mn Sulfuric Acid : Acetonitrile. Isocratic. Ambient. ml/min. Refractive Index min. Conditions: 0:80 t-butyl Alcohol : 0 mm Potassium Phospate Monobasic, ph.. Isocratic. Ambient. ml/min. UV at nm. 0 6 min. Conditions: 9:8. mn Sulfuric Acid : Acetonitrile. Isocratic. Ambient. 0. ml/min. UV 0 nm.

28 8 HPLC COLUMNS AND RESINS Ion Exclusion HPLC Columns Organic Acids Organic Acids Organic Acids Acrylamide, Acrylic Acid and Acrylonitrile PRP-X00,. x 0 mm. Acrylamide, ppm. Acrylic Acid, 0 ppm. Acrylonitrile, 67 ppm Whisky Barrel Congeners PRP-X00,. x 0 mm. Ellagic Acid. Furfural. Hydroxymethyl-. Syringic Acid Furfural. Vanillic Acid 6. Methyl-Furfural 7. Syringaldehyde 8. Vanillin 9. Scopoletin Fermentation Broth PRP-X00. x 0 mm. Lactic Acid. Acetic Acid. Sodium Azide 0. min. Conditions: 9: mn Sulfuric Acid : Acetonitrile. Isocratic. Ambient. ml/min. UV 0 nm min. Conditions: A) 9: mn Sulfuric Acid : Acetonitrile. B) : mn Sulfuric Acid : Acetonitrile. Linear Gradient: 0-0 % B (0 - min.), 0-60 % B ( - min.), 60-6 % B ( - min.). Ambient. ml/min. UV nm. 0 0 min. Conditions: 0 mm Sulfuric Acid. Isocratic. Ambient.. ml/min. UV 0 nm. PRP-X00 Ordering Information PRP-X00, 00 Å Ion Exclusion HPLC Columns I.D. x Length µm 7 µm.0 x 0 mm R x 00 mm R x 0 mm R x 0 mm R x 0 mm R7996. x 0 mm R7997. x 0 mm R7987 R796. x 0 mm R7988 R796. x 0 mm R7989 R796. x 0 mm R796.6 x 0 mm* R797.6 x 0 mm R x 0mm R797 PRP-X00 Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) R7960 R797 Replacement Cartridges (/pk) R79 R797 * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm PRP-X00 Semiprep/Preparative Guard Columns For Steel Columns* Starter Kits ( holder, cartridge) R799 Replacement Cartridges (/pk) R790 * Semiprep/preparative guard column for steel columns is.6 x 0 mm * PEEK hardware Visit the HAMILTON Application Index at product/hplc/myappindex.html or hlpc@hamilton.ch for a complete list of compounds that have been separated.

29 Amino HPLC COLUMNS AND RESINS 9 Amino HPLC Columns HPLC Columns PRP-X700 PRP-X700 polymeric normal phase, aminopropyl columns for separation of carbohydrates. ph stable from 0 to. 7 minute separation of fructose, glucose, sucrose and maltose. Two particle sizes: and 7 µm. Two column internal diameters:. and.6 mm. Two column materials: 6 stainless steel and PEEK. Guard columns in 6 stainless steel and PEEK. Rapid equilibration (0 column volumes). What Types of Samples Can PRP-X700 Columns Separate?... Mono & Disaccharides Corn Syrup PRP-X700,. x 0 mm PRP-X700,. x 0 mm min.. Fructose. Glucose. Sucrose. Maltose Conditions: 8: Acetonitrile : Water. Isocratic. Ambient. ml/min. Injection: µl. Refractive Index min Fructose 6. Maltopentaose. Glucose 7. Maltohexaose. Maltose 8. Maltoheptaose. Maltotriose 9. Maltooctaose. Maltotetraose Conditions: A) Acetonitrile. B) DI Water. Linear Gradient: 90-0 % in 0 min. Flow: ml/min. Injection: 00 µl, 0. % mg/ml Corn Syrup Sample (0. %). Detection: Alltech ELSD 00, 60 psi Nitrogen from Regulator, 0.6 sec. Time Constant. 90 C Drift Tube. Gas Flow:.8 SLPM, 7. psi Nebulizer Gas Pressure, Attenuation.

30 0 HPLC COLUMNS AND RESINS Amino HPLC Columns PRP-X700 Ordering Information PRP-X700, 00 Å Normal Phase HPLC Columns I.D. x Length µm 7 µm. x 0 mm R798 R798. x 0 mm R x 0 mm* R7987 R x 0 mm R7989 PRP-X700 Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) R7980 R798 Replacement Cartridges (/pk) R798 R798 * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm * PEEK hardware

31 HPLC COLUMNS AND RESINS HPLC Bulk Resins Reversed Phase Supports Support Material Exchange Capacity Pore Size µm µm µm 7 µm 0 µm - 0 µm 0-0 µm 0-7 µm PRP- PSDVB* N/A 00 Å R798 R7978 R7979 R7980 R798 R798 R798 PRP- PSDVB* N/A 00 Å R798 R7970 R7970 PRP- Infinity PSDVB* N/A Nonporous R79700 Anion Exchange Supports Support Material Exchange Capacity Pore Size µm µm 7 µm 9 µm 0 µm - 0 µm 0-0 µm 0-7 µm PRP- X00 PSDVB* with Trimethyl-ammonium Exchanger 0.9 meq/gm 00 Å R798 R798 R798 R7986 PRP- X0 PSDVB* with Trimethyl-ammonium Exchanger 0. meq/gm 00 Å R798 R7987 PRP- X00 Poly(methacryl-amidopropyl Trimethylammonium chloride).6 meq/gm Superficially porous R799 R799 R7996 PRP- X600 Poly(dimethyl-amidopropyl-methacrylamide).6 meq/gm Superficially porous R7997 R7998 R7999 RCX-0 PSDVB* with Trimethyl-ammonium Exchanger 0. meq/gm 00 Å R7970 R7970 RCX-0 PSDVB* with Trimethyl-ammonium Exchanger.0 meq/gm 00 Å R7970 R79706 Cation Exchange Supports Support Material Exchange Capacity Pore Size µm µm 7 µm 9 µm 0 µm µm µm 0-7 µm PRP- X00 PSDVB* with Sulfonate Exchanger µeq/gm 00 Å R7987 R7988 PRP- X00 PSDVB* with Sulfonate Exchanger. meq/gm N/A R799 R799 R799 HC-0 Calcium PSDVB* with Sulfonate Exchanger meq/gm Gel-Type R79707** HC-0 Sodium PSDVB* with Sulfonate Exchanger meq/gm Gel-Type R79708** HC-7 Calcium PSDVB* with Sulfonate Exchanger meq/gm Gel-Type R79709 HC-7 Sodium PSDVB* with Sulfonate Exchanger meq/gm Gel-Type R7970 HC-7 Hydrogen PSDVB* with Sulfonate Exchanger meq/gm Gel-Type R797 HC-7 Lead PSDVB* with Sulfonate Exchanger meq/gm Gel-Type R797 Ion Exclusion Supports Support Material Exchange Capacity Pore Size µm µm 7 µm 9 µm 0 µm -0 µm 0-0 µm 0-7 µm PRP- X00 PSDVB* with Sulfonate Exchanger 0.7 meq/gm 00 Å R7986 R7989 R7990 * PSDVB is Poly(styrene-divinylbenzene) ** HC-0 resin is 0 - µm in diameter Bulk resin is sold in gram.

32 Your HAMILTON representative: HAMILTON Bonaduz AG Via Crusch 8 CH-70 Bonaduz Switzerland Tel Fax hplc@hamilton.ch HAMILTON Company 970 Energy Way, Reno Nevada 890, USA Toll Free Tel Fax sales@hamiltoncompany.com A worldwide network of over 00 distributors are available to serve your needs. E/690 0/00 0/0