The Analysis of Organic Acids in Foods and Beverages Using Reversed Phase HPLC
|
|
- Baldwin O’Neal’
- 5 years ago
- Views:
Transcription
1 The Analysis of rganic Acids in Foods and Beverages Using Reversed Phase HPLC Rebecca E. Wittrig & Terrence S. Reid Restek Corporation Benner Circle, Bellefonte, PA 683 USA Restek Corporation restekcorp.com
2 Roles of rganic Acids in Foods & Beverages Flavor Compounds Indicators of Product Quality Purity Determinations using an rganic Acid Profile ph Control in Products Antimicrobial Agents
3 Common Food & Beverage rganic Acids. Acetic acid. Tartaric acid 3. Succinic acid C H 3 4. Malic acid 5. Citric acid H H H H H H H H H H H H H H
4 Challenges in rganic Acid Analysis Specific Assays Column Selection Small carboxylic acids can be difficult to retain by reversed phase HPLC Specialty columns for organic acid analysis Mobile Phase Selection Reversed phase requires a highly aqueous mobile phase Chain folding can occur with highly aqueous mobile phases and conventional C8 columns Reproducibility and Stability The ideal analysis would provide stable and reproducible retention, even with completely aqueous mobile phases
5 Chain Folding Highly aqueous mobile phases can cause chain folding with conventional C8 columns Chain folding can result in a total loss of retention Exposure to completely aqueous mobile phases at ambient pressure (no flow) accelerates the chain folding process
6 Chain Folding on a Conventional C8 Column rganic Mobile Phase Highly Aqueous Mobile Phase
7 Stationary Phases Conventional C8 Ultra Aqueous C8 Ultra IBD Columns with added polar functionalities: enhance the retention of polar compounds eliminate retention loss caused by chain folding (even in % aqueous mobile phase) can provide unique selectivity = polar functionality
8 The Ultra Aqueous C8 Column Provides reproducible retention Provides stability, even when stored in highly aqueous mobile phases Prepared using Type B, high purity silica True C8 phase (USP L)
9 Chain Folding with a Conventional C8 Column A. Initial,,3,4 4 B. After flow stopped 5min. 3 Sample: rganic Acids Peak list:. glycolic acid, 5.4mg/ml. malonic acid, 4.mg/ml 3. acetic acid, 7.8mg/ml 4. maleic acid,.6mg/ml Inj. vol: µl Column: Ultra C8 5 x 4.6mm, 5µm Mobile Phase: 5mM potassium phoshate, ph.5 Flow:. ml/min. Detector: nm 4 6 min 4 6 min
10 Stability of the Ultra Aqueous C8 Column 4 A. Initial B. After flow stopped 5min. 5 Same conditions, except column: Ultra Aqueous C min min
11 Stability of the Ultra Aqueous C8 Column 5 A. Initial 4 B. After 3 Days Same conditions, except column: Ultra Aqueous C min 4 6 min
12 Tobacco Extract: Ultra Aqueous C8 Column 5 Initial Separation 3 4 Sample: Tobacco Extract Peak list:. oxalic acid. pyruvic acid 3. malic acid 4. citric acid Inj. vol: µl Column: Ultra Aqueous C8 5 x 4.6mm, 5µm Mobile Phase: 5mM potassium phoshate, ph.5 Flow:. ml/min. Deector: nm mi
13 Tobacco Extract: Ultra Aqueous C8 Column 5 Injection # Sample: Tobacco Extract Peak list:. oxalic acid. pyruvic acid 3. malic acid 4. citric acid Inj. vol: µl Column: Ultra Aqueous C8 5 x 4.6mm, 5µm Mobile Phase: 5mM potassium phoshate, ph.5 Flow:. ml/min. Detector: nm min
14 Foods & Beverage rganic Acids Standard Sample: Peak List: Standard. Tartaric acid, 6 ppm 8 3. Malic acid, 46 ppm 3. Acetic acid, 6 ppm 4. Citric acid, 58 ppm 5. Succinic acid, 54 ppm 6 Inj. vol: µl Column: Ultra Aqueous C8 5 x 4.6mm, 5µm 4 4 Mobile Phase: 5mM potassium phoshate, ph.5 Flow:. ml/min. Detector: nm min
15 Foods & Beverage rganic Acids Standard 3 Sample: Standard Peak List:. Tartaric acid, 6 ppm. Malic acid, 46 ppm 8 3. Acetic acid, 6 ppm 4. Citric acid, 58 ppm 5. Succinic acid, 54 ppm 6 Inj. vol: µl Column: Ultra Aqueous C x 4.6mm, 5µm Mobile Phase: 98% 5mM potassium phoshate, ph.5 : % acetonitrile Flow:. ml/min. 5 Detector: nm min
16 rganic Acids: Vinegar Sample: Peak List: Inj. vol: µl Column: Mobile Phase: Flow: Detector: 3% Vinegar in Mobile Phase. Acetic Acid Ultra Aqueous C8 5 x 4.6mm, 5µm 5mM potassium phoshate, ph.5. ml/min. nm min
17 rganic Acids: Lemon-Lime Soft Drink Sample: Peak List: Inj. vol: µl Column: % Soda in Mobile Phase. Citric Acid Ultra Aqueous C8 5 x 4.6mm, 5µm Mobile Phase: 5mM potassium phoshate, ph.5 Flow: Detector:. ml/min. nm min
18 rganic Acids: range-mango Juice Sample: Peak List: Inj. vol: µl Column: % Juice in Mobile Phase. Malic Acid. Citric Acid Ultra Aqueous C8 5 x 4.6mm, 5µm Mobile Phase: 5mM potassium phoshate, ph.5 Flow: Detector.:. ml/min. nm min
19 Conclusions ❹ ❹ ❹ The Ultra Aqueous C8 column provides enhanced retention of organic acids Stable and reproducible retention is possible, even with highly aqueous mobile phases Columns with added polar functionalities can be advantageous for analyzing a wide range of polar compounds, including: ❹ ❹ ❹ ❹ Carboxylic acids Vitamins Nutraceuticals Pesticides
Acclaim Mixed-Mode WAX-1 Columns
User Manual Acclaim Mixed-Mode WAX- Columns 06565 Revision 03 October 05 Product Manual for the Acclaim Mixed-Mode WAX- Column Page of 3 Product Manual for Acclaim Mixed-Mode WAX- Columns 5µm, 0 x 50 mm,
More informationCation Exchange HPLC Columns
Cation Exchange HPLC Columns Hamilton offers seven polymeric packing materials for cation exchange separations. Type Recommended Application(s) PRP-X00 PRP-X00 PRP-X800 HC-0 HC-7 Ca + HC-7 H + HC-7 Pb
More informationSimultaneous Determination of Paraquat and Diquat in Environmental Water Samples by HPLC-MS/MS
Simultaneous Determination of Paraquat and Diquat in Environmental Water Samples by HPLC-MS/MS Richard Jack, Xiaodong Liu, Leo Wang, and Chris Pohl OT70806_E 08/13S 1 The world leader in serving science
More informationAcclaim Mixed-Mode WCX-1
Acclaim Mixed-Mode WCX-1 Product Manual for the Acclaim Mixed-Mode WCX-1 Column Page 1 of 28 PRODUCT MANUAL for the Acclaim Mixed-Mode WCX-1 Columns 4.6 x 150 mm, P/N (068353) 4.6 x 250 mm, P/N (068352)
More informationCation Exchange HPLC Columns
Cation Exchange PRP-X00 Polymeric cation exchange packing for separation of inorganic cations and organic cations. Easily separate mono or divalent cations. ph stable from to. Use with organic solvent
More informationNext Generation of Phenyl Columns. Chromatography Products.
Next Generation of Phenyl Columns Chromatography Products RESOLUTION EVOLUTION The Next Generation of Phenyl Column Chemistry The phase is the next generation of phenyl column chemistries, providing both
More informationUltrafast and sensitive analysis of sweeteners, preservatives and flavorants in nonalcoholic beverages using the Agilent 1290 Infinity LC system
Ultrafast and sensitive analysis of sweeteners, preservatives and flavorants in nonalcoholic beverages using the Agilent 129 Infinity LC system Application Note Food and Beverages Author Srividya Kailasam
More informationNovel LC/MS-Compatible Stationary Phase with Polar Selectivity
Novel LC/MS-Compatible Stationary Phase with Polar Selectivity Carmen T. Santasania and David S. Bell Supelco 595 N. Harrison Rd., Bellefonte, PA 16823 T403168 GJU Introduction Stationary phases based
More informationIt s hot! ZIC -chilic
It s hot! ZIC -chilic Complementary selectivity for HPLC and LC-MS separation of polar hydrophilic compounds EMD Millipore Corp. is a subsidiary of Merck KGaA, Darmstadt, Germany ZIC -chilic Your benefits
More informationAlltech Alltima HP Introduction
Alltech Introduction High-Stability, High-Purity, High-Performance, Low-Bleed Columns for Demanding Applications Better Peak Symmetry high-purity silica eliminates peak tailing problems Long Column Life
More informationACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development
ACE C8-Amide For increased polar retention and alternative selectivity Alternative selectivity for method development Improved separations with polar, acidic, basic and phenolic compounds High efficiency
More informationmau 200 ph min
mau ph - 6 8 TAKE A LOOK INSIDE TWIN Technology TWIN (Two-In-One ) Technology is what gives Gei its superior performance edge. During the final stage of silica manufacturing, a unique silica-organic layer
More informationfor Acclaim Mixed-Mode WCX-1
for Acclaim Mixed-Mode WCX- Product Manual for the Acclaim Mixed-Mode WCX- Column Page of 8 Product Manual for Acclaim Mixed-Mode WCX- Columns Acclaim Mixed-Mode WCX-, 3µm, Analytical column, 3.0 x 50mm
More informationgroups I and II cations, amines, divalent transition metals
IC Columns Introduction Alltech IC Column Specifications Column Composition ph Range Applications Anion Exchange Columns Allsep polymer-based anion ph 0 inorganic anions, weak and strong acid ions, exchanger,
More informationHigh Performance Liquid Chromatography. Table 1: Allowed HPLC Adjustment of USP <621> and EP <2.2.46>
High Performance Liquid Chromatography HPLC-9 Ultra-high Speed Analysis of Ibuprofen within USP Allowed Limits by Nexera Method Scouting In recent years, high-throughput analytical techniques have
More informationDetermination of Additives in Carbonated Beverages
pplication Note 9 Determination of dditives in Carbonated everages Introduction The soft drink industry is one of the largest in the world, with revenue from sales of carbonated soft drinks totaling billions
More informationAQUASIL C18 Columns. AQUASIL 18 Columns TG Compatible with 100% aqueous mobile phase
AQUASIL C8 Columns AQUASIL 8 Columns TG0-0 Compatible with 00% aqueous mobile phase AQUASIL OH OH AQUASIL C8 columns provide a versatile C8 phase for a wide range of application areas. AQUASIL C8 columns
More informationNew Applications of Zirconia Based Stationary Phases
ew Applications of Zirconia Based Stationary hases EAS 8 Bingwen Yan 1, Clayton V. Mceff 1, R.A. Henry, Dan owlan 1 1 ZirChrom Separations, Inc. 617 ierce St., Anoka, M 33 Independent Consultant, 983 Greenbriar
More informationFast Analysis of Small MW Analytes in a Beverage and Serum Samples on a Zirconiabased Strong Anion-Exchanger
Fast Analysis of Small MW Analytes in a Beverage and Serum Samples on a Zirconiabased Strong Anion-Exchanger Pittcon 2005 BINGWEN YAN 1, CLAYTON V. MCNEFF 1, 1 ZirChrom Separations, Inc., 617 Pierce Street,
More informationPacked Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS. Table of Contents
No. 089 SEPARATION REPORT Packed Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS Table of Contents 1. Introduction 1 2. Column Specification 1 3. Features of Packing Materials
More informationBETASIL Columns TG Å high purity silica
BETASIL Columns TG0-0 00Å high purity silica Technical Guide BETASIL Columns Introduction 00Å high purity silica High surface area and phase loading Designed for small molecules Type B silica gives good
More informationHPLC Praktikum Skript
HPLC Praktikum Skript Assistants: Gianluca Bartolomeo HCI D330, 3 46 68, bartolomeo@org.chem.ethz.ch Sahar Ghiasikhou HCI E330, 2 29 29, ghiasikhou@org.chem.ethz.ch 1. Introduction In chromatographic techniques,
More informationMulti-mode Separations Using Zirconiabased Stationary Phases
Multi-mode Separations Using Zirconiabased Stationary Phases PITTC 2010 Dan owlan 1, Bingwen Yan 1, Clayton V. Mceff 1, R.A. Henry 2 1 ZirChrom Separations, Inc. 617 Pierce St., Anoka, M 55303 2 Independent
More informationcolumns Acclaim Mixed-Mode WCX-1 for Separating Basic Molecules
columns Acclaim Mixed-Mode WCX- for Separating Basic Molecules The Acclaim Mixed-Mode WCX- is a novel, high-efficiency, silica-based column specially designed for separating various basic analytes. This
More informationFluoroPhenyl. Stationary Phase: Get the Power of HILIC and RP Modes in One LC Column. Pure Chromatography. Selectivity Accelerated
Get the Power of HILIC and RP Modes in One LC Column Selectivity Accelerated Stationary Phase: luorophenyl CH 3 Si CH 3 O Pure Chromatography www.restek.com/raptor The Raptor luorophenyl Column With Raptor
More informationAnalysis - HPLC A.136. Primesep 5 µm columns B.136
Primesep 5 µm columns Primesep columns feature double functionality of the bonding i.e : alkyl chain with anionic or cationic group, chelating group. This feature creates unique selectivities when using
More informationC190-E105 Printed in Japan ATD
C190-E105 Principles and Applications of the Prominence Organic Acid Analysis System Junichi Masuda and Takeshi Goto, Analytical Applications Department Contents 1. Introduction 1 2. What is Ion Exclusion
More informationDetermination of organic acids in fruit juices and white wines
MACHEREY-NAGEL Chromatography application note MN application note 0/0 Determination of organic s in fruit s and white wines MACHEREY-NAGEL application department Dr. H. R. Wollseifen T. Kretschmer Abstract
More informationfor Acclaim Mixed-Mode HILIC-1 Column
for Acclaim Mixed-Mode HILIC-1 Column Product Manual for ACCLAIM Mixed-Mode HILIC-1 Page 1 of 17 Product Manual for ACCLAIM Mixed-Mode HILIC-1 Column 5µm, 4.6 x 250mm, P/N 066844 5µm, 4.6 x 150mm, P/N
More informationToo Polar for Reversed Phase What Do You Do?
Too Polar for Reversed Phase What Do You Do? June 20, 2013 Mark Powell Columns and Consumables Technical Support Page 1 C8 or C18 Doesn t Always Do the Job Typical reversed phase conditions involve water/buffer
More informationSmall Molecule Selectivity Sampler
Small Molecule Selectivity Sampler HALO 90 Å C18, AQ-C18, BIPHENYL Fused-Core Particle Technology Why Choose Fused-Core Particle Technology? Fused-Core Out-Performs Totally Porous Particles. HALO particles
More informationDo you need trouble-free speed? The NEW Chromolith HR
Do you need trouble-free speed? The NEW Chromolith HR EMD Millipore is a division of Merck KGaA, Darmstadt, Germany ... then speed-up to Chromolith HighResolution... Revolutionary monolithic silica columns
More informationcolumns I o n P a c AS11 and AS11-H C Anion-E x c h a n g e C o l u m n s
columns I o n P a c AS and AS-H C Anion-E x c h a n g e C o l u m n s The IonPac AS anion-exchange column provides fast profiling of inorganic anions and organic acid anions using sodium hydroxide or potassium
More informationIC Columns Introduction
IC Columns Introduction Alltech IC Column Specifications Column Composition ph Range Applications Anion Exchange Columns Allsep polymer-based anion ph 0 inorganic anions, weak and strong acid ions, exchanger,
More informationInertSustain AQ-C18. HPLC, LC/MS Columns. Maximizing retention for highly polar compounds in reversed phase methods with highly aqueous mobile phases
HPLC, LC/MS Columns InertSustain AQ-C8 Maximizing retention for highly polar compounds in reversed phase methods with highly aqueous mobile phases Physical Properties Silica : ES (Evolved Surface) Silica
More informationIDENTIFICATION OF STEROIDS IN COSMETIC PRODUCTS BY TLC AND HPLC 1 02/12/2005 ACM 007 A. THIN LAYER CHROMATOGRAPHY (TLC)
Document A. THIN LAYER CHROMATOGRAPHY (TLC) 1. SCOPE AND FIELD OF APPLICATION The method describes the identification of hydrocortisone acetate, dexamethasone, betamethasone, betamethasone 17-valerate
More informationValidated HPLC Methods
Validated HPLC Methods To Tweak or Not to Tweak Slide 4 Adjustments of operating conditions to meet system suitability requirements may be necessary. USP 23 p1776 Slide 5 001816S1.PPT What is the line
More informationFluoroPhenyl. Stationary Phase: Get the Power of HILIC and RP Modes in One LC Column. Pure Chromatography. Selectivity Accelerated
Get the Power of HILIC and RP Modes in One LC Column Selectivity Accelerated Stationary Phase: luorophenyl CH 3 Si CH 3 O Pure Chromatography www.bgb-shop.com/raptor The Raptor luorophenyl Column With
More informationHPLC Reverse Phase Test Mix #1
Analytical Reference Materials HPLC Reverse Phase Test Mix #1 Catalog # 35005 Lot # A083761 110 Benner Circle Bellefonte, PA 16823-8812 (814) 353-1300 FOR LABORATORY USE ONLY. READ MSDS PRIOR TO USE. RAW
More informationcolumns IonPac AS17-C Anion Exchange Column
columns IonPac AS-C Anion Exchange Column The IonPac AS-C is a hydroxide-selective anion exchange column designed for fast gradient separation of inorganic anions. The key application for the AS-C column
More informationThermo Scientific. Acclaim Trinity P2. Column Product Manual. P/N: October Part of Thermo Fisher Scientific
Thermo Scientific Acclaim Trinity P2 Column Product Manual P/N: October 2013 Part of Thermo Fisher Scientific Product Manual for Acclaim Trinity P2 Columns Acclaim Trinity P2, 3µm, Analytical Column, 3.0
More informationAnalyze Nucleotides, Nucleosides, Purine, and Pyrimidine Bases Simultaneously with the Ultra IBD Column
pharmaceutical #9 Applications note Analyze Nucleotides, Nucleosides, Purine, and Pyrimidine Bases Simultaneously with the Ultra IBD Column Mixtures of nucleotides, nucleosides, and their respective purine
More informationReversed Phase Solvents
Part 1. General Chromatographic Theory Part 2. verview of HPLC Media Part 3. The Role of the Mobile Phase in Selectivity Part 4. Column Care and Use Reversed Phase Solvents 2 Solvents for RP Chromatography
More informationPacked columns for reversed-phase chromatography TSKgel ODS-100V and 100Z
Packed columns for reversed-phase chromatography TSKgel ODS-100V and 100Z Contents Page 1. Introduction 2 2. Specifications and characteristics 2 3. Properties of packing material 3 4. Chromatographic
More informationThermo Scientific Syncronis HPLC Columns. Remarkable separations guaranteed time after time
Thermo Scientific Syncronis HPLC Columns Remarkable separations guaranteed time after time Syncronis HPLC and UHPLC Columns Remarkable separations guaranteed time after time When developing a new method,
More informationChemistry 3200 High Performance Liquid Chromatography: Quantitative Determination of Headache Tablets
Chemistry 3200 High Performance Liquid Chromatography: Quantitative Determination of Headache Tablets Liquid chromatography was developed by Tswett in early 1900 s and was shown to be a powerful separation
More informationVirtually Particle-Free Rt -Silica BOND Columns
Petrochemical Applications Virtually Particle-Free Rt -Silica BOND s Provide Reliable PLOT Performance With Less Time Lost for Maintenance By Amanda Rigdon, Bill Bromps, Tom Vezza, and Jaap de Zeeuw Optimized
More informationPackings for HPLC. Packings for HPLC
Summary of packings for HPLC In analytical HPLC, packings with particle sizes of 3 to 10 µm are preferred. For preparative separation tasks, also particles with diameters larger than 10 µm are applied.
More informationCharacterization of Carbon SPE for the Extraction of Polar Analytes
Characterization of Carbon SPE for the Extraction of Polar Analytes Cory Szafranski, Lydia Nolan, and William R. Betz Supelco, Supelco Park, Bellefonte, PA 16823 USA 1997 Sigma-Aldrich Co. T497277 BNG
More informationH 3 CO H 3 CO S CH 3
FENITROTHION 35 H 3 CO P H 3 CO S O CH 3 NO 2 ISO common name Chemical name Fenitrothion O,O-Dimethyl O-4-nitro-m-tolyl phosphorothioate (IUPAC) O,O-Dimethyl O-(3-methyl-4-nitrophenyl)- phosphorothioate
More informationA Systematic Study of the Synthesis of Silver Nanoplates: Is Citrate a. "Magic" Reagent?
SUPPORTING INFORMATION A Systematic Study of the Synthesis of Silver Nanoplates: Is Citrate a "Magic" Reagent? Qiao Zhang, Na Li,, James Goebl, Zhenda Lu, Yadong Yin*, Department of Chemistry, University
More informationYuhui Yang, An Trinh, Michael Ye, and Tom Henderson 595 North Harrison Road, Bellefonte, PA T HKB
Use of An Improved Version of C8+SCX Mixed-Mode Solid Phase Extraction Material for Clean Extraction and Recovery of Basic, Zwitterionic, Neutral and Acidic Compounds from Biological Fluids Yuhui Yang,
More informationDo you need trouble-free speed? The NEW Chromolith HR
Do you need trouble-free speed? The NEW Chromolith HR EMD Millipore is a division of Merck KGaA, Darmstadt, Germany ... then speed-up to Chromolith HighResolution... Revolutionary monolithic silica columns
More informationStreamlining the Analysis of Oral Contraceptives Using the ACQUITY UPLC H-Class System
Streamlining the Analysis of Oral Contraceptives Using the ACQUITY UPLC H-Class System Margaret Maziarz, Sean M. McCarthy, Michael D. Jones, and Warren B. Potts Waters Corporation, Milford, MA, USA A P
More informationColumnsAcclaim PolarAdvantage II (PA2) HPLC Columns
ColumnsAcclaim PolarAdvantage II (PA) HPLC Columns A Unique and Complementary Alternative to Conventional C8.8 Column: Acclaim PA, µm Peaks: ( ppm each) Dimensions:. mm. Maleate Mobile Phase: MeOH/.% NH
More informationOrganic Acids Analysis Kit
Capillary Electrophoresis Organic Acids Analysis Kit PN 5063-6510 2 Upon receipt, please verify that all kit contents listed on page 4 are included. If any part is missing, contact your Agilent Technologies
More informationTEMPLATE FOR AN EXAMPLE STANDARD TEST METHOD
APPENDIX V TEMPLATE FOR AN EXAMPLE STANDARD TEST METHOD Validating Chromatographic Methods. By David M. Bliesner Copyright 2006 John Wiley & Sons, Inc. 159 160 APPENDIX V Title: Effective: Document No:
More informationRevision Bulletin Official April 1, 2014 Alprazolam 1
. Apparatus Official April 1, 2014 Alprazolam 1 1: 100 rpm Alprazolam Extended-Release Tablets s: 1, 4, 8, and 12 h Mobile phase: Acetonitrile, tetrahydrofuran, and Medium (7:1:12) DEFINITION Alprazolam
More informationIntroducing New Functionalities in Liquid Stationary Phases in GC Columns for Confirming Organic Volatile Impurity Testing in Pharmaceutical Products.
Introducing New Functionalities in Liquid Stationary Phases in GC Columns for Confirming Organic Volatile Impurity Testing in Pharmaceutical Products. CHRISTOPHER M. ENGLISH, CHRISTOPHER S. COX, FRANK
More informationGUIDELINES FOR THE DESIGN OF CHROMATOGRAPHIC ANALYTICAL METHODS INTENDED FOR CIPAC COLLABORATIVE STUDY
Page 1 of 13 CIPAC/4105/R GUIDELINES FOR THE DESIGN OF CHROMATOGRAPHIC ANALYTICAL METHODS INTENDED FOR CIPAC COLLABORATIVE STUDY Prepared for CIPAC by Dr M J Tandy*, P M Clarke and B White (UK) The rapid
More informationFast Separation of Vastly Different Compounds by Isocratic HPLC
Fast Separation of Vastly Different Compounds by Isocratic HPLC Aimee N. Heyrman and Yury Zelechonok Resolution Systems, Inc. 590 E. 32nd St. Holland. MI, 49423 SIELC Technologies, 15 E. Palatine Rd, Suite
More informationThermo Scientific Dionex IonPac AS11-HC-4µm Anion-Exchange Column
Chromatography Thermo Scientific Anion-Exchange Column Product Specifications Attain the optimal resolution of organic acids and inorganic anions using the new Thermo Scientific Dionex IonPac AS11-HC-4µm
More informationPRODUCT MANUAL. ACCLAIM OA GUARD COLUMN 4.3 x 10 mm, P/N ACCLAIM OA ANALYTICAL COLUMN 4 x 250 mm, P/N x 150 mm, P/N
PRODUCT MANUAL for the ACCLAIM OA GUARD COLUMN 4.3 x 0 mm, P/N 0695 ACCLAIM OA ANALYTICAL COLUMN 4 x 50 mm, P/N 0690 4 x 50 mm, P/N 06903 QUICKSTART STEPS AND LINKS Click blue text below to get started..
More informationLC and LC/MS Column Selection Flow Chart
LC and LC/MS Column Selection Flow Chart To use the column selection diagram below, simply follow the path for your analyte and mobile phase. At the far right, follow your final column selection to the
More informationADVANTAME. Not less than 97.0% and not more than 102.0% on the anhydrous basis. Sweetener, flavour enhancer
ADVANTAME SYNONYMS INS No. 969 Prepared at the 80 th JECFA (2015), published in FAO JECFA Monographs 17 (2015), superseding tentative specifications prepared at 77 th JECFA (2013). An ADI of 0-5 mg/kg
More informationBRIEFING. (EXC: K. Moore.) RTS C Propylparaben C 10 H 12 O Benzoic acid, 4 hydroxy, propyl ester; Propyl p hydroxybenzoate [ ].
BRIEFING Propylparaben. The European Pharmacopoeia is the coordinating pharmacopeia for the international harmonization of the compendial standards for the Propylparaben monograph, as part of the process
More informationLonicera japonica Flower Dry Extract. Proposed For Development Version 0.1. Published on Herbal Medicines Compendium (
Published on Herbal Medicines Compendium (https://hmc.usp.org) Lonicera japonica Flower Dry Extract Proposed For Development Version 0.1 Lonicera japonica Flower Dry Extract DEFINITION The article is prepared
More informationIon Exchange Columns ---- Inertsil AX 078 Inertsil CX 080. HPLC Column Catalog
Ion Exchange ---- Inertsil AX 078 Inertsil CX 080 HPLC Column Catalog 077 Inertsil AX Physical Properties Silica : Series High Purity Silica Gel : μm Surface Area : 0 m /g Pore Size : 00 Å (0 nm) Pore
More informationSupporting Information
Electronic Supplementary Material (ESI) for ACS Chemical Neuroscience Supporting Information Fluorine-18-labeled Antagonist for PET Imaging of Kappa Opioid Receptors Zhengxin Cai,*, Songye Li, Richard
More informationVALIDATION OF A UPLC METHOD FOR A BENZOCAINE, BUTAMBEN, AND TETRACAINE HYDROCHLORIDE TOPICAL SOLUTION
VALIDATION OF A UPLC METHOD FOR A BENZOCAINE, BUTAMBEN, AND TETRACAINE HYDROCHLORIDE TOPICAL SOLUTION Andrew J. Aubin and Tanya L. Jenkins Waters Corporation, Milford, MA, USA INTRODUCTION Benzocaine (4-Aminobenzoic
More informationDissolution Test 5 was validated using a Zodiac C18 brand of L1 column. The typical retention time for atorvastatin is about min.
Atorvastatin Calcium Tablets Type of Posting Posting Date 25 Jan 2019 Official Date 01 Feb 2019 Expert Committee Chemical Medicines Monographs 2 Reason for Revision Compliance In accordance with the Rules
More informationWhat actually are dietary supplements? They are consumed in large quantities, but they
An Executive Summary Chromatographic Testing of Dietary Supplements following United States Pharmacopeia Monograph Methods Anita Piper Dr. Stephan Altmaier Markus Burholt Michael Schulz Dr. Patrik Appelblad
More informationUSP 35 Official Monographs / Oxaliplatin 4143 DEFINITION
USP 35 Official Monographs / Oxaliplatin 4143 Assay preparation and the Standard preparation, respectively.. Where the test for Uniformity of dosage units has been performed using the Procedure for content
More informationPut the. C18 aside. and take a look... Reasons. to try the. NEW Gemini C6-Phenyl
Put the C18 aside and take a look... 6 Reasons to try the EW Gemini Reason #6 ALTERATE SELECTIVITY to your C18 Column Adding to your method development options, Gemini offers additional polar and aromatic
More informationGB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE
Translated English of Chinese Standard: GB5009.28-2016 www.chinesestandard.net Buy True-PDF Auto-delivery. Sales@ChineseStandard.net NATIONAL STANDARD OF THE GB PEOPLE S REPUBLIC OF CHINA GB 5009.28-2016
More informationDionex IonPac AS28-Fast-4µm column
CHROMATOGRAPHY Thermo Scientific Dionex IonPac AS-Fast-4µm Columns Product Specifications The Thermo Scientific Dionex IonPac AS-Fast-4µm column is a high-capacity, hydroxide-selective anionexchange column
More informationDetermination of Verapamil Hydrochloride Purity Using the Acclaim PA Column
Application Note 95 Determination of Hydrochloride Purity Using the Acclaim PA Column INTRODUCTION -based medications are prescribed for several heart and blood pressure indications. The fastacting formulations
More informationDetermination of Carbonyl Compounds In Water by Dinitrophenylhydrazine Derivatization and HPLC/UV*
Determination of Carbonyl Compounds In Water by Dinitrophenylhydrazine Derivatization and HPLC/UV* EPA Method 8315A UCT Part Number: EUC1812M15 (Unendcapped C18-2000 mg/15 ml cartridge) March 2013 Method
More informationBasic Principles for Purification Using Supercritical Fluid Chromatography
Basic Principles for Purification Using Supercritical Fluid Chromatography Jo-Ann M. Jablonski, Christopher J. Hudalla, Kenneth J. Fountain, Steven M. Collier, and Damian Morrison Waters Corporation, Milford,
More informationRevision Bulletin 27 Jan Feb 2017 Non-Botanical Dietary Supplements Compliance
Niacin Extended-Release Tablets Type of Posting Posting Date Official Date Expert Committee Reason for Revision Revision Bulletin 27 Jan 2017 01 Feb 2017 Non-Botanical Dietary Supplements Compliance In
More informationLC Technical Information
LC Technical Information Method Transfer to Accucore.6 μm Columns Containing solid core particles, which are engineered to a diameter of.6μm and a very narrow particle size distribution; Accucore HPLC
More informationDetermination of Caffeine by HPLC
Determination of Caffeine by HPLC Introduction It was a long history before real high performance liquid chromatography (HPLC) had evolved. The very first indication of a chromatographic separation was
More information[ a ppl ic at ion no t e ]
[ a ppl ic at ion no t e ] Fast A nalysis of A ldehydes and K etones by A C Q U I T Y U P L C Mark E. Benvenuti Waters Corporation, Milford, MA, USA INT RO DUC T ION Aldehydes and ketones are products
More informationMethod Development with ZirChrom's Ion Exchange Phases
For Peak Performance ZIRCHROM ION EXCHANGERS Phases for Sugars and Proteins Wide Range of Ion Exchange Selectivity No Shrinking or Swelling Use Any Organic Solvent Significantly Higher Efficiency than
More informationRevision Bulletin 29 Dec Jan 2018 Non-Botanical Dietary Supplements Compliance
Niacin Extended-Release Tablets Type of Posting Posting Date Official Date Expert Committee Reason for Revision Revision Bulletin 29 Dec 2017 01 Jan 2018 Non-Botanical Dietary Supplements Compliance In
More informationZirconia: the Ideal Substrate for Ion-Exchange LC and LC-MS
Zirconia: the Ideal Substrate for Ion-Exchange LC and LC-MS EAS 2005 Bingwen Yan 1, Clayton V. McNeff 1, Richard A. Henry 2, and David S. Bell 2 1 ZirChrom Separations, Inc., 617 Pierce Street, Anoka,
More informationPrep 150 LC System: Considerations for Analytical to Preparative Scaling
Andrew Aubin and Jo-Ann Jablonski Waters Corporation, Milford, MA, USA APPLICATION BENEFITS The Prep 150 LC System is an affordable, highly reliable system for preparative chromatography and is suitable
More informationRoutine MS Detection for USP Chromatographic Methods
Daniel S. Root, Thomas E. Wheat, and Patricia McConville Waters Corporation, Milford, MA, USA APPLICATION BENEFITS This approach enables mass spectral analysis of peaks directly from unmodified USP HPLC
More informationADVANTAME (TENTATIVE)
ADVANTAME (TENTATIVE) SYNONYMS INS No. 969 New tentative specifications prepared at the 77th JECFA (2013) and published in FAO JECFA Monographs 14 (2013). An ADI of 0-5 mg/kg body weight was established
More informationSepax SRT -C, Zenix -C SEC Phases
Sepax SRT -C, Zenix -C SEC Phases Complimentary Phases to SRT for Derivatized Monoclonal Antibodies General Description Stationary Phase Structure SRT, Zenix, SRT-C and Zenix-C SEC phases developed based
More informationDetermination of 15 phthalate esters by ultra performance convergence chromatography
5th International Conference on Advanced Materials and Computer Science (ICAMCS 2016) Determination of 15 phthalate esters by ultra performance convergence chromatography Wulin Lia, Genrong Li b, Xiao
More informationfor Acclaim Trinity TM P1
for Acclaim Trinity TM P Product Manual for Acclaim TRINITY P Columns Page of 4 Product Manual for Acclaim Trinity TM P Columns 07556 Acclaim Trinity P, 3µm, 4.6 x 00mm 07556 Acclaim Trinity P, 3µm, 4.6
More informationcolumns IonPac SCS 1 Silica Cation Separator
columns IonPac SCS Silica Cation Separator The SCS Silica Cation Separator is designed for use with nonsuppressed conductivity detection, or single-column ion chromatography (SCIC). This column is particularly
More informationAn HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint
An HPLC column offering moderate retentivity with superb peak shape Inertsil ODS Sprint An important new column in the Inertsil series Bonded-Phase Structure Perfect balance of retention for both polar
More informationOBSERVATIONS ON THE WETTING OF REVERSED-PHASE HPLC PACKINGS
1997 Waters Corporation p. 1 980947 OBSERVATIONS ON THE WETTING OF REVERSED-PHASE HPLC PACKINGS T. Walter, P. Iraneta, and M. Capparella Waters Corporation, 34 Maple Street, Milford, MA 01757 USA Presented
More informationC18 Column. Care & Use Sheet
C18 Column Care & Use Sheet HALO Description HALO C18 is a high-speed, high-performance liquid chromatography column based on a new Fused-CoreTM particle design. The Fused-Core particle provides a thin
More informationChange to read: PROCEDURE Buffer: 3.9 g/l of ammonium acetate in water. Adjust
. Blank: Revision Bulletin Official June 1, 2013 Atorvastatin 1 Atorvastatin Calcium Diluent Samples: Sample solution and Blank Instrumental conditions (See Spectrophotometry and Light-Scattering 851.)
More informationRESOLUTION OENO 33/2004 DETERMINATION OF SHIKIMIC ACID IN WINE BY HPLC AND UV-DETECTION
DETERMINATION OF SHIKIMIC ACID IN WINE BY HPLC AND UV-DETECTION The GENERAL ASSEMBLY, Considering Article paragraph iv of the agreement establishing the International organisation of vine and wine Upon
More informationAcclaim AmG C18 Column
CHROMATOGRAPHY Acclaim AmG C8 Column Rugged Reversed-Phase Column Designed for Aminoglycoside Antibiotics Analysis Product Specifications The Thermo Scientific Acclaim AmG C8 column is a high performance
More information