Anion Exchange HPLC Columns

Transcription

1 Anion Exchange PRP-X00 Polymeric anion exchange packings for separation of inorganic and organic anions. Easily separate the eight common anions (fluoride through sulfate). Good separation of fluoride from the water dip. Use with organic solvent from 0 to 00 % for elution of hydrophobic anions or column cleaning. ph stable from to. Use conductivity or UV detection. Three particle sizes:, 0 and -0 µm. Eight column internal diameters:.0 to 0.8 mm. Two column materials: 6 stainless steel and PEEK. Analytical and semiprep/preparative guard columns. Common Anions Anions by Indirect UV Detection EDTA PRP-X00,. x 0 mm, µm (P/N 7980). Fluoride. Chloride. Nitrite. Bromide. Nitrate PRP-X00,. x 0 mm, 0 µm (P/N 79). Fluoride 0 ppm. Carbonate 0 ppm. Chloride 0 ppm. Nitrite 0 ppm. Bromide 0 ppm 6. Nitrate 0 ppm 7. Phosphate 0 ppm 8. Sulfate 0 ppm PRP-X00,.6 x 0 mm, 0 µm (P/N 79). Copper EDTA 0 ppm Conditions: mm p-hydroxybenzoic Acid ph 8. w/.% Methanol ml/min. 00 µl, Conductivity Conditions: mm p-hydroxybenzoic Acid ph 8.9 with. % Methanol. Isocratic. Ambient. ml/min. 00 µl, Indirect UV 0 nm. 0 6 Conditions: mm Sulfuric Acid. Isocratic. Ambient. ml/min. 00 ul, UV nm.

2 PRP-X00 Anion Exchange Organic Acids Germall Bromate and Other Anions PRP-X00,. x 0 mm, 0 µm (P/N 79). Formic Acid 0 µg. Fluoroacetic Acid 0 µg. Isopropylmethyl Phosphonic Acid 0 µg. Chloroacetic Acid 0 µg PRP-X00,.6 x 0 mm, 0 µm (P/N 79),. Germall mg/ml PRP-X00,. x 0 mm, 0 µn (P/N 79). Fluoride 0 ppm. Chloride 0 ppm. Bromate 0 ppm. Bromide 0 ppm. Nitrate 0 ppm 0 0 Conditions: 0.M Sodium Hydroxide in :9 Acetonitrile : Deionized Water. Isocratic. Ambient. ml/min. 0 µl, Conductivity. 0 0 Conditions: 7: 0mM Sodium Hydroxide : Methanol. Isocratic. Ambient. ml/min. 0 µl, UV 0 nm Conditions:.7mM Sodium Bicarbonate,.8mM Sodium Carbonate, 0.mM Sodium Thiocyanate. Isocratic. Ambient. ml/min. 0 µl, Suppressed Conductivity. Sulfite and Sulfate Phosphate and Phosphite Chlorite and Chlorate PRP-X00,.6 x 0 mm, 0 µm (P/N 79). Sulfite.7 mg/ml. Sulfate 0. mg/ml PRP-X00,. x 0 mm, 0 µm (P/N 79). Fluoride 0 ppm. Phosphate 0 ppm. Phosphite 0 ppm. Chloride 0 ppm PRP-X00,. x 0 mm, 0 µm (P/N 79) Fluoride. Chlorite. Chloride. Nitrite. Phosphate 6. Bromide 7. Nitrate 8. Chlorate 0 6 Conditions: 80:0 8mM p-hydroxybenzoic Acid ph 0.0 : Methanol. Isocratic. Ambient. ml/min. 0 µl, Conductivity Conditions: 0mM Succinic Acid ph.9. Isocratic. Ambient. ml/min. 00 µl Conductivity Conditions:.0mM -Amino--Hydroxybenzoic Acid ph.8. Isocratic. Ambient. ml/min.00 µl, Indirect UV 0 nm. I.D. x Length µm 0 µm.0 x 0 mm x 00 mm x 0 mm x 0 mm x 0 mm x 0 mm 79. x 0 mm* x 0 mm x 0 mm 796. x 00 mm x 0 mm 79 I.D. x Length µm 0 µm -0 µm. x 0 mm x 0 mm* x 0 mm* x 0 mm x 0 mm x 0 mm 79. x 00 mm 79. x 0 mm 79. x 0 mm x 0 mm 79 PEEK hardware Analytical Guard Columns For Steel Columns** For PEEK Columns*** Semiprep/Preparative Guard Column For Steel Columns**** Starter Kits Starter Kit 79 ( holder, cartridges) ( holder, cartridge) Replacement Cartridges (/pk) Replacement Cartridges (/pk) 796 ** Analytical guard column for steel columns is. x 0 mm *** Analytical guard column for PEEK columns is.0 x 8.0 mm **** Semiprep/preparative guard column for steel columns is.6 x 0 mm

3 PRP-X0 Anion Exchange Polymeric anion exchange packings for separation of inorganic and organic anions from 0 ppb to 0 ppm. Good separation of fluoride from the water dip. Compatible with conductivity or UV detectors. Use with organic solvent up to 00% ph stable from to. Anions Four column internal diameters:.0 to.6 mm. Polarizable Anions Two column materials: 6 stainless steel and PEEK. Analytical guard columns. Glucaric Acid PRP-X0,. x 0 mm, 7 µm (P/N 797) 6 7. Fluoride ppm. Chloride ppm. Nitrite ppm. Bromide ppm. Nitrate ppm 6. Phosphate ppm 7. Sulfate ppm PRP-X0S,. x 0 mm, 7 µm (P/N 797) 6. Fluoride ppm. Chloride ppm. Iodide 0 ppm. Sulfate 0 ppm. Thiosulfate 0 ppm 6. Perchlorate 0 ppm PRP-X0,. x 0 mm, 7 µm (P/N 797). Glucaric Acid 000 ppm 0 8 Conditions:.0mM p-hydroxybenzoic Acid ph 9.. Isocratic. Ambient. ml/min. 00 µl, Non-Suppressed Conductivity. 0 7 Conditions: mm Sodium Hydroxide with mm Phenol, 0.0mM Sodium Thiocyanate. Ambient.. ml/min. 0 µl, Suppressed Conductivity. 0 6 Conditions: :7 Acetonitrile : 0mM Potassium Phosphate, ph 6.. Isocratic. Ambient.. ml/min. 0 µl, UV 0 nm. PRP-X0 PRP-X0S 7 µm.0 x 0 mm x 00 mm x 0 mm x 0 mm x 00 mm* x 0 mm* x 0 mm* x 00 mm x 0 mm x 0 mm PRP-X0 PRP-X0S 7 µm.6 x 00 mm* x 0 mm* x 0 mm* * PEEK hardware Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm

4 PRP-X00 Anion Exchange Polymeric strong-base anion exchange packing with limited porosity for separation of proteins. Fast separation of large proteins. ph stable from to. Three column internal diameters:. to 0.0 mm. Two column materials: 6 stainless steel and PEEK. Higher sample capacity than nonporous supports. DMT-on Antisense Phosphorothioate 8mer PRP-X00,.6 x 0 mm, 7 µm (P/N 797). DMT-on Phosphorothioate 8mer Proteins PRP-X00,.6 x 0 mm, 7 µm (P/N 797). Myoglobin 7 µg. Conalbumin 7 µg. Dog Albumin 77 µg Conditions: A) 8: 00mM TRIS ph 8.0 : Acetonitrile; B) 8: 00mM TRIS ph.0,.m Lithium Chloride : Acetonitrile. Linear Gradient 0-00% B in 0 min. ml/min. 00 µl of a mg/ml solution, UV 60nm. 0. Conditions: A) 0mM Tris ph 9.0; B) 0mM Tris ph 9.0, 0.N Sodium Chloride. Linear Gradient 0-0% B in. min. Hold for. min. Ambient. ml/min. 0 µl, UV nm.. x 0 mm* x 0 mm 79.6 x 0 mm* x 0 mm* x 00 mm 798 Analytical Guard Column For PEEK Columns* Starter Kit ( holder, cartridges) 799 Replacement Cartridges (/pk) 790 * Analytical guard column for PEEK columns is.0 x 8.0 mm * PEEK hardware 6

5 PRP-X600 Anion Exchange Polymeric weak-base anion exchange packing for gradient separation of proteins and DNA oligomers. Limited porosity allows separation of large DNA fragments. ph stable from to. Column material: PEEK. Guard column in PEEK. Higher sample capacity than nonporous supports. Separation of DNA Fragments DMT-on Phosphorothioate 8mer Proteins PRP-X600,.6 x 0 mm, 7 µm (P/N 7960) PRP-X600,.6 x 0 mm, 7 µm (P/N 7960). DMT-on Phosphorothioate 8mer PRP-X600,.6 x 0 mm, 7 µm (P/N 7960). Myoglobin mg/ml. Concalbumin mg/ml. Ovalbumin mg/ml. Bovine Serum Albumin mg/ml Conditions: A) 0mM TRIS, mm EDTA ph 9.0; B) N NaCl in A, Linear Gradient % B in min. 67.%-7% B (- min.). Ambient. ml/min. µl, UV 60 nm Conditions: A) 8: 00mM TRIS ph 8.0 : Acetonitrile; B) 8: 00mM TRIS ph.0,.m Lithium Chloride : Acetonitrile. Linear Gradient 0-00% B in 0 min. ml/min. UV 60 nm. 0 7 Conditions: A) 0mM TRIS ph 9.0; B) 0mM TRIS, ph 9.0,.0M Sodium Chloride. Linear Gradient 0 to %B in 0 min. Hold min. ml/min. UV 0nm. 7.6 x 0 mm* x 00 mm* x 0 mm* x 0 mm* 7989 * PEEK hardware Analytical Guard Column For PEEK Columns* Starter Kit ( holder, cartridges) 796 Replacement Cartridges (/pk) 796 * Analytical guard column for PEEK columns is.0 x 8.0 mm Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated.

6 RCX-0 Anion Exchange Polymeric anion exchange packing for isocratic or gradient separation of carbohydrates. ph stable from to. Isocratic separation of mono and disaccharides. Gradient separation of oligomers up to DP8. Compatible with PAD, conductivity and RI detectors. Three column internal diameters:. to.6 mm. Two column materials: 6 stainless steel and PEEK. Guard columns in stainless steel and PEEK. Glycols in Water Acetyl Glucosamine Artichoke Tubers RCX-0,. x 0 mm, 7 µm (P/N 790). Ethylene Glycol 00 ppb. Propylene Glycol 00 ppb 0 8 Conditions: 0mN Sodium Hydroxide. Isocratic. Ambient. ml/min. 0 µl, Pulsed Amperometric, Dual Gold Electrode. E=60 mv T=66 msec E=900 mv T=66 msec E=-800 mv T=00 msec RCX-0,. x 0 mm, 7 µm (P/N 790). Galactosamine 00 ppm. Glucosamine 00 ppm. Acetyl-Glucosamine 00 ppm 0 7 Conditions: 0mN Sodium Hydroxide (purged with Helium during the run) Isocratic. Ambient. ml/min. 0 µl, Refractive Index. RCX-0,. x 0 mm, 7 µm (P/N 790). DP. DP. DP0. DP 0 0 Conditions: A) 60mM Sodium Hydroxide; B) 60mM Sodium Hydroxide with 00mM Sodium Acetate. Gradient: 0-00%B in 0 min. ml/min. 0 µl, Pulsed Amperometric, Dual Gold Electrode E=00 mv T= msec E=800 mv T=66 msec E=-600 mv T=99 msec. x 0 mm x 0 mm x 0 mm* 7988 Analytical Guard Columns For Steel Columns* For PEEK Columns** Starter Kits ( holder, cartridges) Replacement Cartridges (/pk) * Analytical guard column for steel columns is. x 0 mm ** Analytical guard column for PEEK columns is.0 x 8.0 mm 8

7 RCX-0 Anion Exchange Polymeric anion exchange packing for isocratic or gradient separation of complex carbohydrates. ph stable from to. Isocratic or gradient modes. Higher ion exchange capacity support for separation of closely related carbohydrates. Compatible with PAD and RI detectors. Three column internal diameters:.,. and.6 mm. Two column materials: Stainless steel and PEEK. Guard column in PEEK. Glycoprotein Monosaccharides Corn Syrup Artichoke Tubers RCX-0,.6 x 0 mm, 7µm (P/N 7970). Fucose. Galactosamine. Glucosamine. Galactose 6. Glucose 6. Mannose RCX-0,.6 x 0 mm (P/N 7970) Glucose. Fructose. Maltose. Maltotriose. Maltotetraose 6. Maltopentaose 7. Maltohexaose 8. Maltoheptaose 9. Maltooctaose RCX-0,.6 x 0 mm (P/N 7970). Glucose. Fructose. Sucrose. DP. DP0 6. DP Conditions: 6mN Sodium Hydroxide. Isocratic. Ambient. ml/min. µl, Pulsed Amperometric µafs Dual Gold Electrode E=600 mv T=66 msec E=600 mv T=66 msec E=-800 mv T=9 msec Conditions: A) 60mM Sodium Hydroxide; B) 00mM Sodium Acetate in A. Linear Gradient 00% A for min., then 0-00% B (- min.) Ambient. ml/min. 0 µl. Pulsed Amperometric Dual Gold Electrode. E=0 mv T=66 msec E=900 mv T=66 msec E=-80 mv T=00 msec Conditions: A) 60mM Sodium Hydroxide; B) 00mM Sodium Acetate in A. Linear Gradient: 0-0% B in 0 min., then 0-00% B (0-0 min.). Ambient. ml/min. 0 µl, Pulsed Amperometric Dual Gold Electrode. E=0 mv T=66 msec E=900 mv T=66 msec E=-800 mv T=00 msec 9 Analytical Guard Column For PEEK Columns*. x 0 mm 7970 Starter Kit ( holder, cartridges) 797. x 0 mm 7980 Replacement Cartridges (/pk) x 0 mm* 7970 * Analytical guard column for PEEK columns is.0 x 8.0 mm.6 x 0 mm* * PEEK hardware Visit the HAMILTON Application Compound Index at or chromatography@hamiltoncompany.com for a complete list of compounds that have been separated.