columns IonPac AS18 Anion-Exchange Columns

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1 columns IonPac AS Anion-Exchange Columns The IonPac AS is a hydroxideselective anion-exchange column designed for the determination of inorganic anions and low-molecularweight organic acids including fluoride, acetate, formate, chloride, nitrite, bromide, nitrate, sulfate, and phosphate. The AS column can be used with isocratic hydroxide eluents or hydroxide gradients for the determination of inorganic anions. The AS column is recommended for use in combination with the EG40 or EG0 Eluent Generators, which automatically produce potassium hydroxide eluents from water. The capacity and selectivity of the AS column provide the flexibility to modify the eluent conditions for optimum separation of the anions of interest. The AS column is ideal for use with isocratic hydroxide eluents for fast separation of the common inorganic anions in simple sample matrices. The high capacity of the AS column allows the use of hydroxide gradients and large-loop injections to determine low levels of inorganic anions in complex sample matrices, including drinking water and wastewater samples. The IonPac AS column, using hydroxide eluents, meets the performance requirements specified in U.S. EPA Method (A). The U.S. EPA Office of Water has approved hydroxide eluents and hydroxideselective columns for compliance monitoring of inorganic anions in drinking water and wastewater samples in accordance with EPA Methods and The common inorganic anions can easily be separated in a variety of sample matrices, including drinking water, wastewater, process streams and scrubber solutions. The AS selectivity provides excellent retention of fluoride from the water dip and baseline resolution of fluoride, acetate, and formate. Solvent compatibility permits easy column cleanup after the analysis of samples with hydrophobic components.

2 Recommended for Inorganic Anions in Diverse Sample Matrices Source water and drinking water Municipal and industrial wastewater Industrial cooling water Hazardous waste extracts and dump site leachates Acid rain Foods and beverages Anionic counterions in pharmaceutical preparations and synthetic peptides Polymers such as polyols and polysulfonates Scrubber solutions Superior Chromatographic Performance Fast isocratic separation of the common inorganic anions in 9 min. Hydroxide gradients for inorganic anion and low-molecular-weight organic acids for optimum separations in complex sample matrices. Superior retention and quantification of fluoride, acetate, and formate. Meets performance requirements specified in U.S. EPA Method (A). Ideal alternative for AS4A-SC, AS2A, AS4, AS4A, and AS inorganic anion applications. Simplified Reagent-Free IC operation provided by the EG40 or EG0 Eluent Generators, which require only a deionized water source to produce potassium hydroxide eluent. Eluent suppression using the ASRS ULTRA or Atlas AAES technology provides Reagent-Free IC operation with low backgrounds and enhanced analyte sensitivity. High capacity: 2 µeq/ col. (4 20 mm column). Operate at ambient or elevated temperatures. Column selectivity is optimized for a 30 C operating temperature to ensure reproducible retention times. Compatible with organic solvents to enhance analyte solubility, modify column selectivity, or for effective column cleanup. Ethylvinylbenzene-Divinylbenzene Core Figure. Structure of an IonPac AS packing particle. 2 Eluent: 39 mm Potassium hydroxide Temperature: 30 C 3 4 Inj. Volume: 2 µl ASRS ULTRA 4 mm, 0 ma, 2 AutoSuppression recycle mode Peaks:. Fluoride mg/l (ppm) 2. Chloride 2 3. Nitrite 4. Sulfate. Bromide 0 -. Nitrate Phosphate Figure 2. Fast isocratic separation of the common inorganic anions on an IonPac AS column using a 39 mm potassium hydroxide eluent delivered by an Eluent Generator. High-Efficiency Particle Structure The IonPac AS packing is a unique pellicullar structure composed of a highly cross-linked core and a MicroBead latex anion-exchange layer attached to the surface, as shown in Figure. The substrate for the IonPac AS analytical column is a.-µm diameter macroporous resin bead, consisting of ethylvinylbenzene Anion-Exchange Latex Coating -nm Diameter MicroBeads 922 cross-linked with % divinylbenzene. The anion-exchange layer is functionalized with very hydrophilic quaternary ammonium groups. This latex bead anion-exchange layer has a controlled thickness, which results in excellent mass transfer characteristics and consequently highly efficient peaks.

3 Fast, Isocratic Separation of Common Inorganic Anions The AS provides the fast separation of fluoride, chloride, nitrite, bromide, nitrate, phosphate, and sulfate using an isocratic potassium hydroxide eluent and suppressed conductivity detection. Using a 39 mm potassium hydroxide eluent, the common inorganic anions can easily be separated in 9 min, as illustrated in Figure 2. The isocratic hydroxide eluent can be modified easily to resolve the common inorganic anions, plus low-molecular-weight organic acids and oxyhalides. Figure 3 shows the separation of these analytes eluting in min using an optimized 33 mm potassium hydroxide eluent. Hydroxide Gradients for Complex Sample Matrices The AS can be operated with hydroxide gradients for optimum separation of inorganic anions in complex sample matrices. The IonPac AS is an ideal column for compliance monitoring of drinking water and wastewater. The AS meets the requirements of U.S. EPA Method Part A. As shown in Figure 4, fluoride is well resolved from the system void and can be determined even at very low concentrations. Low levels of inorganic anions can easily be determined in drinking water using an optimized hydroxide gradient, as illustrated in Figure 4. Wastewater can be a more complex sample than drinking water; however, with the AS column, inorganic anions in wastewater can easily be determined using a fast dual-hydroxide gradient, as illustrated in Figure. Eluent: 33 mm Potassium hydroxide Temperature: 22 C (ambient) Inj. Volume: 2 µl ASRS ULTRA 4 mm, 00 ma 9 0 Peaks:. Fluoride 0. mg/l (ppm) Acetate Formate Chlorite. Chloride 3. Carbonate 20. Nitrite Sulfate 0 9. Bromide Nitrate 0. Chlorate 0 2. Phosphate Figure 3. Optimized isocratic separation of the common inorganic anions, plus organic acids and oxyhalides on an IonPac AS column, using a 33 mm potassium hydroxide eluent delivered by an Eluent Generator Figure 4. Determination of inorganic anions in a drinking water sample on an IonPac AS column using a potassium hydroxide gradient delivered by an Eluent Generator Eluent: Potassium hydroxide: mm at min Inj. Volume: 00 µl ASRS ULTRA 4 mm, 00 ma, AutoSuppression recycle mode Peaks:. Fluoride 9. Carbonate 2. Acetate 9. Bromide Unknown 0. Sulfate 4. Formate. Nitrate. Chloride 2. Chlorate Nitrite 3. Phosphate. Unknown Eluent: Potassium hydroxide: 2 44 mm from 0 min and 44 2 mm from 0 min Inj. Volume: 00 µl ASRS ULTRA 4 mm, 0 ma Sample Prep: Wastewater sample diluted 00: Peaks:. Fluoride 2. Acetate 3. Formate 4. Unknown. Unknown. Unknown. Unknown. Chloride 9. Nitrite 0. Carbonate. Sulfite 2. Sulfate 3. Bromide 4. Unknown. Nitrate. Unknown. Unknown. Thiosulfate 9. Unknown Figure. Determination of inorganic anions in a wastewater sample on an IonPac AS column using a potassium hydroxide gradient delivered by an Eluent Generator.

4 The AS column provides excellent separation of a variety of environmental anions including inorganic anions, oxyhalides (excluding bromate), oxyanions, and organic acids using a potassium hydroxide gradient. With a fast dualhydroxide gradient, these analytes are easily separated in less than 4 min, as illustrated in Figure. Gradient Separations as Simple as Isocratic Runs with the EG40 or EG0 Eluent Generators The IonPac AS is recommended for use in combination with the EG40 or EG0 Eluent Generators. The EG40 and EG0 Eluent Generators electrolytically produce high-purity potassium hydroxide eluent from water, eliminating the need for eluent preparation. The potassium hydroxide eluent is free of carbonate contamination. Carbonate-free hydroxide eluents minimize baseline shifts during hydroxide gradients, which provide greater retention time reproducibility, lower background conductivity, and lower consistent detection limits for target analytes. Figure illustrates the determination of anions in a well water sample using a hydroxide gradient with the Eluent Generator for eluent delivery. A CR-ATC Continuously Regenerated Anion Trap Column was used to remove carbonate from the source water to minimize the baseline shift during the gradient. This sample contains unusually high concentrations of nitrate, which exceed the allowed concentrations in drinking water. Figure illustrates the determination of anions in a bottled spring water using a hydroxide gradient with the Eluent Generator for eluent delivery. This sample contains high concentrations of chloride, carbonate, and sulfate. Acetate and formate were also present in the sample which may be caused by bacterial contamination or may have been added as salts for flavor Eluent: Potassium hydroxide: 2 44 mm from 0 min and 44 2 mm from 0 min Inj. Volume: 2 µl ASRS ULTRA 4 mm, 0 ma, Autosuppression 9 recycle mode Peaks:. Fluoride Bromide 0.0 mg/l 2. Acetate 2.. Selenate Formate.0 2. Nitrate Chlorite.0 3. Chlorate 0.0. Chloride Phosphate 0.0. Nitrite.0. Molybdate 0.0. Selenite 0.0. Tungstate 0.0. Sulfite 0.0. Arsenate Sulfate 0.0. Thiosulfate Chromate Figure. Anion separation including inorganic anions, organic acids, oxyanions, and oxyhalides on an IonPac AS column using a potassium hydroxide eluent delivered by an Eluent Generator. 4 Column: Eluent: Eluent Source: IonPac AS 4 mm Potassium hydroxide: 2 30 mm in min EGC-KOH with CR-ATC Figure. Determination of inorganic anions in a well water sample on an IonPac AS column using a hydroxide gradient delivered by an Eluent Generator Figure. Determination of anions in a bottled drinking water sample on an IonPac AS column using a potassium hydroxide gradient delivered by an Eluent Generator. 0 Inj. Volume: 2 µl AAES, 00 ma, Sample: Brentwood, CA well water Peaks:. Fluoride mg/l (ppm) 2. Acetate 3. Unknown 4. Chloride 9. Nitrite Carbonate 23. Bromide 0.9. Sulfate 2 9. Nitrate Phosphate Eluent: Potassium hydroxide: 2 30 mm in min Inj. Volume: 00 µl AAES, 00 ma, Sample: Commercial bottled water 3 Peaks:. Fluoride mg/l Acetate 3. Formate Unknown. Unknown. Chloride.. Carbonate Sulfate.2 9. Nitrate Oxalate 903

5 Ordering Information For optimum ease-of-use and economy, the IonPac AS column can be used with either the ASRS Anion Self-Regenerating Suppressor or the Anion Atlas Electrolytic Suppressor (up to 2 µeq/min). We recommend operating the IonPac AS column at an elevated, controlled temperature (30 C) to ensure reproducible retention times. The EG40 or EG0 Eluent Generator is used to automatically produce potassium hydroxide gradients from deionized water. When using the EG40 or EG0 Eluent Generator for eluent delivery, a CR-ATC Continuously Regenerated Anion Trap Column should be installed between the EluGen cartridge and the Eluent Generator degas module. As an alternative, an ATC-HC column can be installed between the pump outlet and the inlet of the EluGen Cartridge in the EG40 or EG0 Module. Alternatively, when performing sodium hydroxide gradient anionexchange applications on the AS, using hand-prepared bottled eluents, the ATC-3 Anion Trap Column should be installed between the gradient pump and the injection valve to remove anionic contaminants from the eluent. For concentrator work, use the IonPac AG guard column when a single-piston pump such as the DQP or DXP pump is used for sample delivery. Use the TAC-LP Anion Concentrator Column when the sample is delivered with a syringe or with an autosampler such as the AS40. SPECIFICATIONS Dimensions: IonPac AS Analytical Column: 2 20 mm 4 20 mm Dimensions: IonPac AG Guard Column 2 0 mm 4 0 mm Maximum Operating Pressure: 4000 psi Mobile Phase Compatibility: ph 0 4; 0 00% HPLC solvents Substrate Characteristics: Analytical Column: 2 20 and 4 20 mm Super macroporous resin Bead Diameter:. µm Pore Size: 2000 Å Cross-linking (%DVB): % Guard column: 2 0 and 4 0 mm Microporous resin Bead Diameter: 3 µm Pore Size: < Å Cross-linking (%DVB): % Latex Characteristics: Functional Group: Alkanol quaternary ammonium ion Latex Cross-linking: Effective crosslinking after functionalization is %. Latex Diameter: nm Hydrophobicity: Low hydrophobic Capacity: Analytical Column (2 20 mm): µeq Guard Column (2 0 mm): 2. µeq Analytical Column (4 20 mm): 2 µeq Guard Column (4 0 mm): 0 µeq Column Construction: PEEK with 0-32 threaded ferrule-style end fittings. All components are nonmetallic.

6 PART NUMBERS To order in the U.S., call , or contact the Dionex Regional Office nearest you. Outside the U.S., order through your local Dionex office or distributor. Refer to the following part numbers. Analytical and Guard Columns IonPac AS Analytical Column (4 20 mm)... P/N 0049 IonPac AG Guard Column (4 0 mm)... P/N 00 IonPac AS Analytical Column (2 20 mm)... P/N 003 IonPac AG Guard Column (2 0 mm)... P/N 00 Trap Columns CR-ATC Continuously Regenerated Anion Trap Column (For use with the EG0 with built-in CR-TC control, the EG40 with EG40 CR-TC Add-On Kit or the RFC-30 Reagent-Free Controller)... P/N 004 ATC-3 Anion Trap Column (9 24 mm) (for use with 4-mm columns)... P/N 090 ATC-3 Anion Trap Column (4 3 mm) (for use with 2-mm columns)... P/N 09 ATC-HC Anion Trap Column (for use with the EG40 or EG0)... P/N 0904 Trace Anion Concentrator TAC-LP Trace Anion Concentrator (4 3 mm)... P/N 0402 MicroBead and Reagent-Free are trademarks and ASRS, Atlas, EluGen and IonPac, and Reagent Free are registered trademarks of Dionex Corporation. Printed on recycled and recyclable paper. Dionex Corporation Dionex Corporation Dionex U.S. Regional Offices Dionex International Subsidiaries 22 Titan Way Salt Lake City Technical Center Sunnyvale, CA (40) 3-22 Austria (0) 2 Belgium (03) Canada (90) China (2) Denmark P.O. Box 303 West 2200 South, Suite A Westmont, IL (30) 9-30 France Germany Italy (0) 0 2 Japan (0) -23 The Netherlands (0) Sunnyvale, CA Salt Lake City, UT Houston, TX (2) 4-2 Switzerland (02) United Kingdom (02) Atlanta, GA (0) * Designed, developed, and manufactured under an NSAI registered ISO 900 Quality System. (40) (0) Marlton, NJ () LPN 3 M 2/ Dionex Corporation