columns IonPac AS22 Anion-Exchange Column

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1 columns IonPac AS Anion-Exchange Column The IonPac AS is a carbonate based anion-exchange column designed for the determination of inorganic anions and low-molecular-weight organic acids including fluoride, acetate, chloride, nitrite, bromide, nitrate, phosphate, and sulfate. The AS column is used with isocratic carbonate/bicarbonate eluents and suppressed conductivity detection. The AS column can be used in combination with Dionex Eluent Generators and the Electrolytic ph Modifier (EPM), which automatically produce potassium carbonate/bicarbonate eluents from water. The IonPac AS-Fast column is ideal for fast IC using eluent generation or eluent regeneration. Column Performance The IonPac AS column meets the performance requirements specfied in U.S. Environmental Protection Agency (EPA) Method 00.0 (A). The common inorganic anions are easily separated in a variety of sample matrices, including drinking water, wastewater, process streams, and scrubber solutions. The AS selectivity provides excellent retention of fluoride from the water dip and resolution of fluoride, acetate, and formate. Solvent compatibility permits easy column cleanup after the analysis of samples with hydrophobic components. Fast IC The IonPac AS-Fast is ideal for Fast IC as it is designed to have sufficient capacity to maintain resolution even in a short column format. Fast separations are achieved on any Dionex system at higher flow rates. In a short column format, backpressures produced at higher flow rates are reduced while allowing overall shorter run times. This allows for the determination of anions with high resolution even in drinking, surface, groundwater, and wastewater matrices in under min. Laboratories can achieve higher productivity and increased throughput. Passion. Power. Productivity.

2 Analyze Inorganic Anions in Diverse Sample Matrices Source water and drinking water Municipal and industrial wastewater Industrial cooling water Hazardous waste extracts and dump site leachates Acid rain Foods and beverages Anionic counterions in pharmaceutical preparations and synthetic peptides Polymers such as polyols and polysulfonates Scrubber solutions Superior Chromatographic Performance Fast isocratic separation of the common inorganic anions in simple sample matrices in 8 min. Isocratic separation of common inorganic anions in complex sample matrices in min. High speed separation of the common inorganic anions in less than min using the AS-Fast Column. Carbonate peak well resolved from common inorganic anions. Meets performance requirements specified in EPA Method 00.0 (A). Ideal alternative for IonPac ASA- SC, ASA, AS, and ASA inorganic anion applications. Simplified Reagent-Free ion chromatography (RFIC ) System operation provided by Dionex Eluent Generators and EPM, which require only a deionized water source to produce potassium carbonate/bicarbonate eluent. Ideal column for use with Dionex Eluent Regeneration, enabling simple, non-stop operation for up to 8 days. Simple, accurate eluent preparation with the AS Eluent Concentrate just dilute in deionized water and start operation. Eluent suppression using the ASRS 00 or AAES technology provides Reagent-Free IC operation with low backgrounds and enhanced analyte sensitivity. Conceptual Illustration of Surface Modification Figure. Structure of an IonPac AS column particle. Column: IonPac AG, AS, mm Eluent:. mm sodium carbonate/. mm sodium bicarbonate Temperature: 0 C Flow Rate:. ml/min Inj. Volume: 0 µl Detection: Suppressed conductivity, ASRS 00, mm, Figure. Separation of common inorganic anions plus acetate on an IonPac AS column using a. mm carbonate/. mm bicarbonate eluent. High capacity: 0 µeq/col. ( 0 mm column). Operate at ambient or elevated temperatures. Column selectivity is optimized for a 0 C operating temperature to ensure reproducible retention times. Compatible with organic solvents to enhance analyte solubility, modify column selectivity, or for effective column cleanup. Peaks:. Fluoride mg/l (ppm). Acetate 0. Chloride 0. Nitrite. Bromide. Nitrate. Phosphate 0 8. Sulfate

3 High Efficiency Particle Structure The IonPac AS column was developed using a unique polymer bonding technology. The stationary phase consists of a novel hyper-branched anion-exchange condensation polymer, electrostatically attached to the surface of a wide-pore polymeric substrate. The substrate is surface-sulfonated in exactly the same manner as is common in Dionex latex coated anion-exchange materials. However, in this anionexchange resin, alternating treatments of epoxy monomer and amines produce a coating which is grown directly off the substrate surface as illustrated in Figure. Resin capacity is controlled through the number of alternating coating cycles. The AS mm uses a high-capacity resin (0 µeq/column) with optimized selectivity for the common inorganic anions in a variety of sample matrices. Isocratic Separation of Common Inorganic Anions The AS provides excellent separation of fluoride, chloride, nitrite, bromide, nitrate, phosphate and sulfate using an isocratic carbonate/bicarbonate eluent and suppressed conductivity detection. Using a. mm carbonate/. mm bicarbonate eluent, the common inorganic anions plus acetate can be resolved in approximately min as shown in Figure. Column: IonPac AG, AS, mm Peaks:. Fluoride 0.8 mg/l (ppm). Bromide 0.0 Eluent:. mm sodium carbonate/. Formate Nitrate mm sodium bicarbonate. Chloride.9 9. Carbonate NQ Temperature: 0 C. Nitrite Phosphate 0. Flow Rate:. ml/min. Unknown NQ. Sulfate 0. Inj. Volume: µl. Chlorate 0.8 Detection: Suppressed conductivity, ASRS 00, mm, NQ: Not Quantified Figure. Determination of inorganic anions in a municipal drinking water sample on an IonPac AS column Inorganic Anions in Drinking Water The IonPac AS is ideal for compliance monitoring of drinking water and wastewater. The column meets or exceeds the performance requirements of U.S EPA Method 00.0 (A). Common inorganic anions are separated in approximately min in drinking water as shown in Figure.

4 Fast IC The IonPac AS-Fast column is suitable for separations requiring higher flow rates for the fast analysis of inorganic anions in simple sample matrices as shown in Figure. The IonPac AS- Fast column ( 0 mm) was operated with the same eluent at. and.0 ml/min. Using a higher flow rate in a shorter column format, the overall run time was reduced to. min with optimal resolution of the common inorganic anions. The use of an AS-Fast 0 mm column format allows greater linear velocities and reduced eluent consumption compared to a mm format. Figure shows the excellent resolution for the common inorganic anions in less than min. Column: IonPac AS-Fast ( 0 mm) Eluent:. mm sodium carbonate. mm sodium bicarbonate Flow Rate: A.. ml/min B..0 ml/min Inj. Volume: 0 µl Temperature: 0 C Detection:.0.0. A B Suppressed conductivity, ASRS 00, mm, Peaks:. Fluoride.0 mg/l (ppm). Chloride 0.0. Nitrite.0. Bromide.0. Nitrate.0. Phosphate 0.0. Sulfate Figure. Separation of common inorganic anions using the IonPac AS-Fast 0 mm column. Column: IonPac AS-Fast ( 0 mm) Eluent:. mm sodium carbonate. mm Sodium bicarbonate Flow Rate: A. 0. ml/min B. 0. ml/min C. 0. ml/min Inj. Volume:. µl Temperature: 0 C Detection: Suppressed conductivity, ASRS 00, mm, A) Peaks:. Fluoride.0 mg/l (ppm). Chloride 0.0. Nitrite.0. Bromide.0. Nitrate.0. Phosphate 0.0. Sulfate B) C) Figure. Separation of common inorganic anions using the IonPac AS-Fast 0 mm column.

5 Figure illustrates the analysis of a municipal drinking water sample using the AS-Fast ( 0 mm). At.0 ml/min, the anions can be separated in less than min. The bottom trace shows an enlarged image of the separation. The anions are well resolved even in the presence of disparate anion concentrations including fluoride/formate and chloride/nitrite. Extended Application Capabilities The unique selectivity and high capacity of the AS column makes it ideal for methods development of specialized anion applications. Figure shows the separation of a variety of environmental anions including inorganic anions, oxyhalides, oxyanions, and organic acids using an isocratic carbonate/bicarbonate eluent. These 8 analytes are easily separated in less than 0 min. Column: IonPac AS-Fast ( 0 mm) Eluent:. mm sodium carbonate. mm sodium bicarbonate Flow Rate:.0 ml/min Inj. Volume: 00 µl Temperature: 0 C Detection: Suppressed conductivity, ASRS 00, mm, Peaks:. Fluoride. Bromide. Formate. Nitrate. Chloride 8. Carbonate. Nitrite 9. Phosphate. Chlorate 0. Sulfate Figure. Analysis of a municipal drinking water sample using the IonPac AS-Fast 0 mm column. S Column: IonPac AG, AS, mm Eluent:.8 mm sodium carbonate/.0 mm sodium bicarbonate Flow Rate:. ml/min Temperature: 0 C Inj. Volume: 0 µl Detection: Suppressed conductivity, ASRS 00, mm, Peaks:. Fluoride mg/l (ppm). Acetate 0. Formate 0. Bromate 0. Chloride. Nitrite. Chlorate 8. Bromide 9. Nitrate 0. Phosphate 0. Sulfate 0. Selenate 0. Iodide 0. Arsenate 0. Thiocyanate 0. Perchlorate 0. Thiosulfate 0 8. Chromate Figure. Anion separation including inorganic anions, organic acids, oxyanions, and oxyhalides on an IonPac AS column.

6 The IonPac AS column is also ideal for the determination of anions in food and beverage samples containing high concentrations of carbonate. Carbonated bottled water contains up to 00 mg/l (ppm) of carbonate. The unique selectivity of the AS column positions carbonate well away from the common inorganic anions and does not interfere with quantification of the analytes of interest. Figure 8 shows the determination of inorganic anions in carbonated water using the AS column. The AS column can be used to evaluate the mass balance of drugs and synthetic peptide preparations. Figure 9 illustrates the use of the AS column to determine the anionic counterion amount and type. The common inorganic anions plus trifluoroacetate can be determined in less than min Column: IonPac AG, AS, mm Eluent:. mm sodium carbonate/. mm sodium bicarbonate Temperature: 0 C Flow Rate:. ml/min Inj. Volume: 0 µl Detection: Suppressed conductivity, ASRS 00, mm, 0.0 Peaks:. Fluoride 0.9 mg/l (ppm). Chloride.8. Bromide 0.9. Nitrate.. Carbonate NQ. Phosphate 0.. Sulfate. NQ: Not Quantified Figure 8. Determination of inorganic anions in carbonated water on an IonPac AS column. Column: IonPac AG, AS, mm Eluent:. mm sodium carbonate/. mm sodium bicarbonate Temperature: 0 C Flow Rate:. ml/min Inj. Volume: 0 µl Detection: Suppressed conductivity, ASRS 00, mm, Peaks:. Fluoride mg/l (ppm). Acetate 0. Chloride. Trifluoroacetate 0. Bromide 0. Nitrate 0. Phosphate 0 8. Sulfate Figure 9. Analysis of the common inorganic anions and TFA using the IonPac AS column.

7 SPECIFICATIONS Dimensions: IonPac AS Analytical Column: 0 mm, 0 mm IonPac AS-Fast Analytical Column: 0 mm, 0 mm IonPac AG Guard Column: 0 mm, 0 mm IonPac AG-Fast Guard Column: 0 mm, 0 mm Maximum Operating Pressure: 000 psi Mobile Phase Compatibility: ph 0 ; 0 00% HPLC solvents Substrate Characteristics: Analytical Column: Supermacroporous resin Bead Diameter (µm):. µm Pore Size: 000 Å Crosslinking (%DVB): % Guard Column: Microporous resin Bead Diameter (µm): µm Pore Size: < Å Crosslinking (%DVB): % Ion-Exchange Group: Functional Group: Alkanol quaternary ammonium ion Functional Group Characteristics: Hydrophobicity: Ultralow hydrophobic Capacity:. µeq ( 0 mm column). µeq ( 0 mm column) 0 µeq ( 0 mm column) µeq ( 0 mm column) µeq ( 0 mm column).0 µeq ( 0 mm column). µeq ( 0 mm column).0 µeq ( 0 mm column) Column Construction: PEEK with 0 threaded ferrule style end fittings. All components are nonmetallic. ORDERING INFORMATION System Requirements for EGC-K CO /KHCO Eluent Generation For K CO /KHCO eluents generated using the EGC-K CO cartridge and the EPM, use with ICS-00 or ICS-000 IC systems equipped with an Eluent Generator. The Eluent Generator and EPM are used to automatically produce potassium carbonate/bicarbonate eluents from deionized water. The EGC- K CO cartridge can only be controlled using Chromeleon. and subsequent Chromeleon releases. Suppressor Recommendations For optimum ease-of-use and economy, the IonPac AS column should be used with the ASRS Anion Self-Regenerating Suppressor, ASRS 00 or the Anion Atlas Electrolytic Suppressor, AAES. It is recommended to operate the IonPac AS column at an elevated temperature (0 C) to ensure reproducible retention times. Concentrator Columns For concentrator work, use the IonPac AG guard column, UTAC-LP, UTAC-ULP, UTAC XLP, UTAC-LP, UTAC-ULP, or UTAC-XLP, Ultra Trace Anion Concentrator Columns, TAC-LP or TAC-ULP Trace Anion Concentrator Column, TAC- Trace Anion Concentrator Column, or AMC- Anion Microconcentrator when a single-piston pump such as the AXP- pump (pulse damper required) is used for sample delivery. Use the UTAC-LP, UTAC-LP, Ultra Trace Anion Concentrator Column or TAC-LP Trace Anion Concentrator Column when the sample is delivered with a syringe or with a lowpressure autosampler such as the AS-DV. Product Description Part Number IonPac AS Analytical Column... 0 ( 0 mm) IonPac AG Guard Column ( 0 mm) IonPac AS Analytical Column... 0 ( 0 mm) IonPac AG Guard Column... 0 ( 0 mm) IonPac AS-Fast Analytical Column ( 0 mm) IonPac AG-Fast Guard Column ( 0 mm) IonPac AS-Fast Analytical Column ( 0 mm) IonPac AG-Fast Guard Column ( 0 mm) AMC- Anion MicroConcentrator ( mm) TAC- Trace Anion Concentrator ( mm) TAC-LP Low Pressure Trace Anion Concentrator ( mm) TAC-ULP Ultra Low Pressure Trace Anion Concentrator ( mm) UTAC-LP Ultra Trace Anion Concentrator Low Pressure ( mm) UTAC-ULP Ultra Trace Anion Concentrator Ultra Low Pressure... 0 ( mm) UTAC-XLP Ultra Trace Anion Concentrator Extremely Low Pressure ( mm) UTAC-LP Ultra Trace Anion Concentrator- Low Pressure ( mm) UTAC-ULP Ultra Trace Anion Concentrator- Ultra Low Pressure ( mm) UTAC-XLP Ultra Trace Anion Concentrator- Extremely Low Pressure ( mm) AS Sodium Carbonate/Bicarbonate Eluent Concentrate (0 ml of 00X concentrate)

8 Reagent-Free and RFIC are trademarks and ASRS, Atlas, Chromeleon and IonPac are registered trademarks of Dionex Corporation. PEEK is a trademark of Victrex Corporation. Passion. Power. Productivity. Dionex Corporation North America Europe 8 Titan Way U.S./Canada (8) 9-00 Austria () Benelux () ; () 9 Australia () 90 China (8) 8 8 India (9) P.O. Box 0 Denmark () France () Germany (9) 99 0 Japan (8) 88 Korea (8) 80 Singapore () Sunnyvale, CA South America Ireland () 00 Italy (9) 0 Sweden () 8 80 Taiwan (88) Brazil () 0 Switzerland () 0 99 United Kingdom () 9 LPN 8-0 0/0 (08) Dionex Corporation Asia Pacific