South China Fisheries Science , ; 2., g ( Pelteobagrus fulvidraco), 4,

Transcription

1 South China Fisheries Science Vol. 9, No. 6 Dec., 2013 doi: / j. issn ,,,,, ( 1., ; 2., ) : g ( Pelteobagrus fulvidraco), 4, 0 ( ) 100 kg mg mg kg mgkg - 1 8,,, 400 mgkg % 7. 5%20. 3% ( P < 0. 05) ( P > 0. 05),,,, mgkg - 1 ( P < 0. 05) ; ( P > 0. 05) 400 mgkg - 1, ; 200 mgkg - 1 : ; ; ; ; : S 963 : A : ( 2013) Effects of complete enzyme preparation on growth performance, serum biochemical and immune indices of juvenile yellow catfish ( Peltobagrus fulvidraco) WANG Guoxia, LIU Qunfang, HUANG Wenqing, LIN Jianan, HUANG Yanhua, XU Liming ( 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou , China; 2. Guangzhou Fishtech Fisheries Science & Technology Co., Ltd. Guangzhou , China) Abstract: We randomly assigned 360 juvenile yellow catfish ( Peltobagrus fulvidraco) with average body weight g to 4 groups, and fed the control ( G0) with basal diet, the other 3 groups ( G100, G200 and G400) with basal diet supplemented with 100 mgkg - 1, 200 mgkg - 1 and 400 mgkg - 1 complete enzyme preparation ( CEP), respectively. After feeding for 8 weeks, the weight gain rate ( WGR), specific growth rate ( SGR) and feed intake ( FI) increased with the CEP concentration. Besides, the WGR, SGR and FI of G400 were higher than those of the control by 15. 4%, 7. 5% and 20. 3% ( P < 0. 05), respectively. No significant difference was found among all the treatments in body moisture, crude protein, crude lipid and ash content ( P > 0. 05). Serum urea content decreased with increasing CEP in diets, while glutamic -pyruvic transaminase ( ALT), glutamic -oxalacetic transaminase ( AST) activities and total protein( TP) content increased ( P > 0. 05). The activity of lysozyme ( LSZ) increased and then decreased( P < 0. 05). In conclusion, the feed added with 400 mgkg - 1 CEP can promote the growth : ; : : ; : ( ),,,, @ qq. com : ( ),,,, com

2 6 : 85 and ALT activities of yellow catfish, and decrease its urea content; the feed added with 200 mgkg - 1 CEP can significantly increase the TP content and LSZ and AST activities of the fish. Key words: Pelteobagrus fulvidraco; complete enzyme preparation; growth ; biochemical indices; immunity ( Pelteobagrus fulvidraco),,, 2, ( 0 ) ( ) g,, 450, 4, 3,, 30 2, 9: 00 1] 16: 30, 4% 6% 8,,, 24 h, 28 32, ph ,, ( ) > 5mgL - 1, ( ) < mgl - 1, (,, ) < mgl - 1,, ], 12 h,,, 3], 5, 1 ml,, 3 4 h, ( 4, rmin - 1, ( Ctenopharyngodon idellus ) 4-5] ( Cyprinus carpio) 6] ( Carassius auratus) 7] ( Large- mouth bass) 8] ( Oreochromis niloticus) 9] ] ( , 40% ) ( GB/ T ), 898A ( ) ( GB/ T ),,,, ( GB/ T ), 550 ( GB/ T ), , ( CHO) ( TG) ( GLU) 1, 1. 1 ( TP),,,,, ( G0 )100 mgkg kg mg mgkg - 1 3, G100G200 G ,, SLX-80 ( 2), G400, % ( P < 0. 05), 1. 2, 0. 05) 15 min), ( ) ( ALT) ( AST) ( LZM) ( SOD) ( MDA) mm, 55, 15. 4% 20. 3% ( P < 0. 05) ( P >

3 ( ) Tab. 1 Feed formula and nutritional level ( air dry basis) % ingredient content ingredient content fish meal 5 vitamin premix soybean meal 25 mineral premix rapeseed meal 25 ( 50% ) choline chloride 0. 5 cottonseed meal 25 lysine 0. 7 soy protein concentrate 9 lysine 0. 7 corn starch 2 V C coated vitamin C 0. 1 fish oil 1. 7 ( 98% ) betaine 0. 5 soybean oil 1. 7 cm-cellulose-na 2 total 100 nutrient level moisture 7. 0 crude lipid 5. 0 crude protein ash 7. 5 : 1. ( gkg - 1 ) : V A IU; V D IU; V E 16 ; V K 3; V B1 4; V B2 8 ; V B ; V B12 16 mg; 28; 16; 1. 28; 64 mg; ( gkg - 1 ) : 230 ; 36; 9; 10; 8; 1. 9 ; 1. 5; 250 mg; 32 mg; 50 mg Note: 1. Provided by the Fishtech Fisheries Science & Technology Co., Ltd.. One kilogram of vitamin premix contains: V A IU; V D IU; V E 16 g; V K 4 g; V B1 4 g; V B2 8 g; V B g; V B12 16 mg; nicotinic acid 28 g; calcium pantothenate 16 g; folic acid g; biotin 64 mg; inositol 40 g. 2. Provided by the Fishtech Fisheries Science & Technology Co., Ltd.. one kilogram of mineral premix contains: Ca 230 g; K 36 g; Mg 9 g; Fe 10 g; Zn 8 g; Mn 1. 9 g; Co 250 mg; I 32 mg; Se 50 mg. 2 Tab. 2 Effects of complete enzyme preparation on growth performance and feed utlization of yellow catfish index G0 G100 G200 G400 / g IBW / g FBW b b b a / % WGR b ab ab a / %d - 1 SGR b ab ab a / g - 1 FI b ab b a FCR : ( P < 0. 05), Note: Different superscripts in the same row indicate significant difference( P < 0. 05). The same case in the following tables. 2. 2, ALT G400, ( P < 0. 05), AST G200, ( P > 0. 05) ( G100 ( 4) 3) 2. 3 TP G200,, CHOTG 2. 4 ( P < 0. 05) LZM GLU, ( P > 0. 05), G100 G200 G100 G400, G400 ( P < 0. 05) SOD MDA ALT AST ( P > 0. 05) ( 5)

4 6 : 87 3 ( ) Tab. 3 Effects of complete enzyme preparation on body composition of yellow catfish ( dry weight) % index G0 G100 G200 G400 CP EE ash moisture Tab. 4 Effects of complete enzyme preparation on serum biochemical indices of yellow catfish index G0 G100 G200 G400 c( ) / mmoll - 1 CHO c( ) / mmoll - 1 TG c( ) / mmoll - 1 GLU c( ) / mmoll - 1 UREA a b a b / UL - 1 ALT b ab ab a / UL - 1 AST b b a ab 5 Tab. 5 Effects of complete enzyme preparation on serum immune indices of yellow catfish index G0 G100 G200 G400 ( ) / mgml - 1 TP b ab a ab / UL - 1 LZM b a a b / UmL - 1 SOD b( ) / nmolml - 1 MDA ,,, ( Takifugu obscurus) ( 3. 1, ), 6. 5% 6. 2% 15. 4%, 3. 4% 3. 0% 7. 5%, 12],, 400 mgkg - 1, 13] 14] 10],,,, 400 mgkg - 1,, 4, 6 ] ], ( C. auratus gibelio) ( TG) ( GLU),,,

5 88 9,,,, ( ALT),, 15] 16] ALT, ;, : 1 ]. J]., ( Orechromis niloticus O. aureus), 2010( 3) : ], 2 ],. J]., 2012, 36 ( 1) : , 3 ],,,. J]., 2009, 28( 12) : , 4 ],,,. J]., , 24( 5) : ],,,. ( TP) J]., 2010 ( 12) : , 6 ],,,., J]., 2008, 23 ( 5 ) : ,, 7 ]. J].,, 2001, 22( 3) : mgkg ],,,. ( Litopenaeus vannamei) 18 ] J]., 2008, 29 ( 6) : 21 -, ],. PS,, 19 ] J]., 2005, 27 ( LZM) ( 4 ) : ,,, LZM,, 2002, 15 ( 1) : ], ],,,. mgkg - 1 LZM, J]., 2008,, 38( 1) : ] 12],,,. 21] 11] J] ], ( SOD), 14],. J]., 2011, 32( 8) : 1-4.,, ( O - 15]. 2 ) ( H 2 O 2 ) ( O 2 ), 22] ( MDA) ph J]., 2001, 23 ( 3 ) :,, 15]. :, 2009: 165, SOD MDA 10],. J]., 2012, 21 ( 2) : ( 8) : J]., J]., 2012, 40 ( 17 ) : ], SOD MDA, 17]. 9] 21 ], D]. :, ] SOD, 18],,,., SOD M]., 19] ELLIS A E. Immunity to bacteria in fish J]. Fish & Shellfish Im

6 6 : 89 munol, 1999, 9 ( 4) : ] BAYNE C J, GERWICK L. The acute phase response and innate immunity of fish J]. Dev & Comp Immunol, 2001, 25 ( 8 /9 ) : ],,,. J]., 2008, 35( 3 ) : ] FANG Y Z, YANG S, WU G. Free radicals, antioxidants, and nutrition J]. Nutrition, 2002, 18( 10) : ,,,, ( CSCD) RCCSE,, ( ),, 16, 96, 5 12, 6 72 : 46-65, : 231 : : : : : http: www. schinafish. cn nfsc@ vip com