Growth, molt, and maturation of shrimp brood stock Penaeus

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1 JOURNAL OF FISHERIES OF CHINA Vol. 26, No. 1 Feb.,2002 : (2002) ,, (, ) :,, ( 1 1), 90 %, 12d, 3d,, 7d, 30d,,,, 14 15d,,,20d, 0. 25mm, 0. 17mm,,0. 20mm,0. 15mm : ; ; ; ; ;; :S : A Growth, molt, and maturation of shrimp brood stock Penaeus vannamei cultured in the river mouth area in Shanghai CAI Sheng2li, ZANG Wei2ling, DAI Xi2lin ( Fisheries College, Shanghai Fisheries University, Shanghai , China) Abstract :A recirculating system was designed for shrimp brood stock Penaeus vannamei culture indoor in the low salinity area of the river mouth in Shanghai. The natural seawater with salinity of 9-12 was mixed with concentrated seawater with salinity of , which made the culture water salinity reach About 200 shrimp brood stock (female was about 1 1) were cultured in the recirculating system for two months, and the survival rate of the shrimp was about 90 %. stimulation of the ovary maturation. Unilateral eyestalk ablation of female shrimp was done for Three days after the operation the ovary development could be observed clearly, and one week later, a part of shrimp began to spawn, but the eggs were not fertilized. One month later fertilized eggs appeared and nauplius were obtained. The results showed that shrimp brood stock P. vannamei cultured in this recirculating system could live healthily and spawn successfully. growth in shrimp brood stock P. The regularity of molting and vanamei was investigated. Before ablation of eyestalk, the mean molt interval : :[ (00) 1-18 ] :( ),,,,, Tel : , E2mail edu. cn

2 48 26 was about days, and after that, the mean molt interval extends to about 20 days due to ovary development. With molting the body length of shrimp brood stock increased continuously, whether before or after ablation of eyestalk, but the mean daily increase of body length before ablation (female :0. 25mm, male : mm) is greater than that after ablation (female :0. 17mm, male : 0. 15). The reasons of lower growth rate and longer molting interval were discussed. Key words : Penaeus vannamei ; brood stock ; molt ; growth ; maturation ; river mouth ; water recirculating system ( Penaeus vannamei),,, [1-3 ] 1988,,,, [4 ], ( P. ( P. japonicus) ( P. monodon) chinensis) ( Macrobrachium rosenbergii),,,,,,,,,,, [5,6 ] [7 ],,,,,,,,,,, [8 ],,, (1),,,, ; (2), , 105, cm, 100, cm 4, 10,,, Q 1. 2 ( ) 3 20, 12d, 27 28, 27 28, Lx,p H ,, 5 17

3 1 : 49 58d ( Nereis spp. ) ( ), ( Smonovacula spp. ), 4 1,7 :30 17 :30, 5 % 10 % 1. 3,, (9 12),,, 27 28, 50 %, ( 1) 1 Fig. 1 Sketch of the recirculating system A :, m 2, 1m B : B 1, (100 ) B 2 10 C : C 1 :, C 2 :, C 3 :,C 4 : D :, 1 000W ( WaterTch,:UV12AS) B 2 C 4 1,2t h h 2t h - 1, 6 8h 1 DP : ECB : WDM : A :Hatching tank, ellipse, depth : 1m, area : B : Egg2collecting case. A egg2collecting bag is set in B layer of polythene nets are set in B 2. C : Biological filter. C 1 : multi2zeolites, C 2 :plastic balls, C 3 : zeolites, C 4 :polythene net. D : Germicide light. UV2light, 1 000W ( WaterTec made in USA, Model :UV12AS). A submersible pump with a 2t h - 1 of flow rate is set in B 2 and B 4. The UV light is on for 8-10h day - 1, and the total water can be circulated once in 6-8h. DP :diving pump ECB : egg collecting bag WDM : water draining mouth h,, ( ) 28 30, (10 15) 10-6 g ml - 1, 24h, NaS 2 O 3,,, (10 15) 10-6 g ml - 1 EDTA2Na 8 :00 p H COD 60cm,,,,, 20cm, 12 12h,, lx,,,, 1,,,, 17 :00-22 :00, ,, 4 2, mg L - 1,, 1mg L - 1, ( ),, mg L - 1,2mg L - 1, 4mg L - 1,

50 26,1 2mg L - 1,,, 20cm NH 3 - N NO - 2 - N,, 3 20 12h, 5 17, ( 1), :95. 1 % 98. 5 % 94. 3 %,, Tab. 1 Survive rate of P.

4 50 26,1 2mg L - 1,,, 20cm NH 3 - N NO N,, h, 5 17, ( 1), :95. 1 % % %,, Tab. 1 Survive rate of P. 1 vannamei during experimental period ( - ) date (M - D) before transportation after transportation after temporary culture after spawning ( ) number of shrimp ( ) number of dead ( %) survive rate , 4, 2 (58d),,1. 07cm, 0. 90cm ( P < 0. 01), (12d) 12d ( P > 0. 05) ( 2),, (0. 25mm) 1 (0. 21mm), ,, 0. 08mm,, 2 1 Fig. 2 Growth of P. vannmei during experiment period 0. 20mm 0. 14mm,, 0. 18mm ( 2),, ( ), 3 p H,, 5 %,,,, 5 %, 192, 162,13. 5, %, % 12d,84 %

5 1 : 51 2 Tab. 2 Growth of P. vannmei during experimental period ( - ) date (M - D) female male / cm body length / mm increase per day / cm body length / mm increase per day (a) (a) (b) (b) (a) (a) (b) (b) : ( P > 0. 05), ( P < 0. 01), ( F = , F = ) Notes : The different letters in the same line show significant difference ( P < 0. 01), and the same letters show insignificant different ( P > 0. 05), ( F female = , F male = 9. 55), 7 %, 2 3d,,, 14 15d d, 440,, 10, % 2. 3, d, Fig. 3 Molting of P. vannmei before eyestalk ablation 5 6d 1 16d ( ),, 2 20d ( ),, ( ), 4 ( ) Fig. 4 Molting of P. vannmei after eyestalk ablation

6 d,6 7d, 30d,, 457, 137, 33 % ( 3),87. 5 % 3 Tab. 3 Spa wn, fertilization, and hatch rate of P. vannamei ( - ) date (M - D) before fertilizing after fertilizing 10 4 number of eggs number of fertilized eggs ( %) fertilizing rate 10 4 nauplius ( %) hatching rate : 3 :the hatching rate of the fertilized eggs ,,,28 35, 9 12,,, (28 ),,,,,,1m 3. 2, (1) NH 3 2N NO 2 2N :,,, NH 3 2N NO 2 2N,,,,, (2) :, 28,,,, (3) :,, (1 1), ( 10 ), (4) :,,,,, 90 % (5) :,,, 3. 3,,

7 1 : 53,,,, 0. 2mm,,,,,,,7 %5 %, 14d 20d,,,, 1cm 6 7g, 7 %, 25 %,,, - X (MIH) ( GIH),,, [9-12 ],,,,, Quackenbush :, [11 ],,;,:, - X MIH GIH, GIH,,, Y -,,, ( ), GIH - X,, : [1 ] Zhang W Q. Introduction of biology of Penaeus vannamei, the important culture species in the world[j ]. Marine Sciences, 1990, (3) : [. [J ].,1990, (3) : ] [2 ] Jiang H L, You E M, Zhu L H, et al. Artificial breeding technique on Penaeus vannamei[j ]. J Shanghai Fish Univ, 1999, (3) : [,,,. [J ].,1999, (3) : ] [3 ] Lin Z S, Gao Q L, Gao D Y. Studies on techniques of the artificial breeding of Penaeus vannamei in BohaiBay[J ]. Marine Sciences, 1997 (6) : [,,. [J ].,1997(6) : ] [4 ] Zhang W Q, Yu L J, Tong B F, et al. Studies on the full2artificial fertilization of Penaeus vannamei [J ]. Oceanol et Limnol Sin, 1993, 24 (4) : [,,,. [J ].,1993,24(4) : ] [ 5] Lu G H, Pan J P, Chen J M, et al. Preliminary study and economic analysis of pond culture for shrimp Penaeus vannamei[j ]. Fisheries Science & Technology Information, 2000, 27 (1) : [,,,. ( Penaeus vannamei) [J ]., 2000,27 (1) : ] [ 6] Xu G F. Rapid culture of shrimp Penaeus vannamei in low salinity seawater[j ]. Modern Fisheries Information, (1) : [. [J ].,2000 (1) : ] [7 ] Zeng D S. Adaptation of shrimp Penaeus vannamei to freshwater [J ]. Fisheries Science & Technology Information, 2000, 27 (5) : [. [J ].,2000,27 (5) : ] [8 ] Isao Y. Final oocyte maturation,spawning and mating in penaeid shrimp [J ]. J Exp Mar Biol Ecol, 1995,193 : [9 ] Chen S, Malone R F, Huner J V. Molting and mortality of red swamp and white river crawfish subjected to eyestalk ablation : a preliminary study for commercial soft2shell crawfish production[j ]. J World Aquacult Soc, 1993,24(1) : [10] Choy S C. Growth and reproduction of eyestalk ablated Penaeus canaliculatus (Oliver, 1811) ( Crustacea : Penaeidae) [J ]. J Exp Mar Biol Ecol, 1987,112 : [11 ] Quackenbush L S, Herrnkind W F. Regulation of molt and gonadal development in the spiny lobster, Panulirus argus ( Crustacean : Palinuridae) : effect of eyestalk ablation[j ]. Comp Biochem Physiol, 1981, 69 : [12] Lin R R, He J J, Qiu H S. Inducement on ovarian development, maturation and spawning in Penaeus monodon fabricius grow2out from earth ponds[j ]. J Fish China, 1990, 14 (4) : [,,. [J ]., 1990,14 (4) : ]

Advances in se* determination of shrimps prawns and crabs

13 2 Vol.13 No.2 2004 6 J0URNAL 0F SHANGHAI FISHERIES UNIVERSITY June 2004!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! 1004-7271 2004 02-0157 - 07 Advances in se* determination of shrimps