47 (6) : , Higgins 2/ 3. ( Higgins et al., 1931) (Michalopoulos et al., 1997) Sprague2Dawley ( Ratt us norvegicus) (

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1 47 (6) : A cta Zoologica S inica 3 ( ) (partial hepatectomy PH) ( G0 G1) (4 h) (36 h) (successive partial hepatectomy SPH) ( 4 / 36 h) ; ; SPH 4 ( E G K M ) (short in2 terval successive partial hepatectomy SISPH) 1931 Higgins 2/ 3 ( Higgins et al. 1931) (Michalopoulos et al. 1997) Sprague2Dawley ( Ratt us norvegicus) ( ( ) g ) 112 (Michalopoulos et al ; 2001 ; 2001 ; 2001 ; ) ; 2001 ; 2001) cm 4 ( 1 ) 4 h / 36 h ( 3 3 ) ( 1997) h ( 4 h / 36 h 1988) ( 72 ( 3 ) h) ( 4 ) 1 20 ( ( ) 42 : E2mail : edu. cn

2 ( ) ( 10 m % 75 % 85 % ) H. E ( 1985) 75 % ; 9215 % ( 24 h 1991) P = ; P = ; ( 6 m 20 8 ) Feulgen ( 1998) P = ( 1) SPSS : E E () : SPSS ( : a) 1 Table 1 Relationship between the frequency total amount of liver resected and interval between successive resections and the %rate of rat survival ( %) (h) ( %) Groups Amount of liver resected Frequency of resection Hepatic lobe resected Interval between resections Survival rate A B C D E F G H J K L M : 1 (Lobus externalis sinister resected in first time) : 2 (Lobus centralis sinister and lobus2 centralis resected in second time) : 3 (Lobus dexter sinister resected in third time) : 4 (Lobus caudatus sinister resected in forth time)

3 6 : h ; 3 4 h 1 2 ( 1 2 ) ( ) K h (: b) 2 4 h ; 3 36 h ; 4 36 h ( : c d) 3 4 h 2 ( ) M h 1 ; ; (: e f) G h 4 h 2 ; ; 2 4 h 4 36 h ; ; 1 ( )

4 ( P E G K M ( 1) SPSS 12 : P = ; 75 % P = (4 h) (long interval successive partial hepatectomy L ISPH) ; Fig. 1 Changes of rate of the binuclear cells by SISPH ( PH) : E ( Group E) : G ( Group G) : ( PH 4 h) : K ( Group K) : M ( Group M) DNA ( PH 24 h) ( PH h) ( PH 48 h) ( Fabrikant 1968) E G K M ( 2) ( short SPSS interval successive partial hepatectomy SISPH) SISPH ( ) PH 2 Fig. 2 Changes of mitotic index by SISPH : E (group E) : G (group E) : K (group E) : M (group E) = 01008) ( P = 01002) 3 (36 h) ( E G K M) ( 1997) ( PH) 3 ( 115 ) L ISPH ( ) ( 1985) A B C D

5 6 : % 80 % 9215 % ; (2) ; (3) 85 % 3 ( ) E E F G H (3 ) (85 %) 1 36 h ( ) E ( Fab2 3 rikant 1968) 4 J K L M 15 3 : 4 3 ( ) ( E G J L ) K L E J G L (24 h) 3 4 D ( 9215 %) J K L M ( %) J K 10 % ( Higgins 1931) G K M 30 % ( 1989) ( 1988) E : G K M : (1) ( ) ; (2) ; (3) : (1) ( )

6 ( References) Duan R. F. J. F. Chang D. F. Zhang M. Xia and C. S. Xu 2001 Effect of short interval successive partial hepatectomy (SISPH) on hepa2 tocyte nucleus and nucleolus. Acta Zool. Si n. 47 (Suppl. ) : [ 2001 (SISPH). 47 ( ) : ] Er Z Technique of Cell and Tissue Culture. Beijing : People Health Press 141. [ : 141. ] Fabrikant J. L The kinetics of the cellular proliferation in the regenerating liver. J. Cell Biol. 36 : Feng Q. C. A. L. Lu G. W. Li and C. S. Xu 2001 Effects of four times (interval 36 h) successive partial hepatectomy on the activity and content of ACP A KP HSC70/ HSP68 and PCNA in rat live. Acta Zool Si n. 47 (Suppl. ) : [ 2001 ( 36 h) ACP A KP HSC70/ HSP68 PCNA. 47 ( ) : ] Higgins G. M. and R. M. Anderson 1931 Experimental pathology of the liver.. Restoration of the liver of white rat following partial surgical removal. A rch. Pathol. 12 : Hu Y. H. J. L. Guo and C. S. Xu 2001 Primary purification of proteinases ADAMs ACP A KP and PCNA in rat liver by ion exchange chromatography. Acta Zool. Si n. 47 (Suppl. ) : [ 2001 ADAMs ACP A KP PCNA. 47 ( ) : ] Kun L. F. W. Niu and C. X. Rong 1991 Basic Embryology and Histology. Shanghai : Shanghai Science and Technique Press 153. [ : 153. ] Li Y. H. Y. H. Hu J. L. Guo Y. W. Han and C. S. Xu 2001 Effect of different factors on the neutral proteinase activity of rat liver during SPH. Acta Zool. Si n. 47 (Suppl. ) : [ 2001 SPH. 47 ( ) : ] Liang W. H. J. T. Lin Y. C. Li and C. S. Xu 2001 The differential display of the expression of adam genes in short interval successive partial hepatectomy (SISPH). Acta Zool. Si n. 47 (Suppl. ) : [ 2001 (SISPH) ADAMs. 47 ( ) : ] Michalopoulos G. K. and M. C. DeFrances 1997 Liver regeneration. Science 275 : Sun K. J. and Y. Q. Liu 1985 Histological observation during liver regeneration after partial hepatectomy in different extent. J. A na. 19 (3) : [ (3) : ] Wu Y. P. and Z. D. Wu 1997 Constraction of the model of the rat partial hepatectony again and again and it significance. J. Ex p. S urgery of Chi na 14 (1) : [ (1) : ] Xia M. R. F. Duan J. F. Chang D. F. Zhang and C. S. Xu 2001 Changes of glycogen Glucose262phosphatase and RNA in liver dur2 ing the short interval succesive partial hepatectomy. Acta Zool. Si n. 47 (Suppl. ) : [ 2001 (SISPH) G262Pase RNA. 47 ( ) : ] Xu C. S. M. Xia and A. L. Lu 1999 Studies of the change about HSC70/ HSP68 proteinases and phosphatases during liver regeneration. Acta Physiol. Si nica 51 (5) : [ (5) : ] Xu C. S. Y. H. Li Y. H. Hu Y. W. Han and J. L. Guo 2001 Changes of proteinase activities during SISPH of rat liver. Acta Zool. Si n. 47 (Suppl. ) : (SISPH). 47 ( ) : ] Yang H. M. [ Experiment of Cell Biology (Second edition). Beijing : Higher Education Press 75. [ 1998 ( ). : 75. ] Zhou J. X. M. Xia and S. Jin 1989 Rapid isolation and primary culture of the rat hepatocytes. J. Henan Normal U ni. 16 (2) : [ (2) : ] Zhu J. H. L. Z. Cheng and C. P. Zhong 1988 Study of proliferative cycle of rat hepatocytes after partial hepatectomy using flow cytometry. Acta A na. Si nica 19 (4) : [ (4) : ]

7 6 : 665 ( Abstract) EFFECTS OF THE S HORT INTERVAL SUCCESSIVE PARTIAL HEPATECTOMY ON RAT SURVIVAL AND L IVER TISSUE STRUCTURE 3 XU Cun2Shuan L I Yong2Hui DUAN Rui2Feng L U Ai2Ling XIA Min J I Ai2Ling ( College of L if e Sciences Henan Normal U niversity Xi nxiang Henan Chi na) In the paper we analyzed 4 types of short interval successive partial hepatectomy (SISPH) models ( E G K and M) were established after the possibility of that were analyzed with 12 types of SISPH models. The ef2 fects of SISPH on rat survival and its liver tissue structure were observed with histological method. According the results of A D models when the amount of liver once resected is up to 46 % 75 % or 85 % the survival rates of rat ( Ratt us norvegicus) were above 75 % but when t he amount of liver once resected was up to 9215 % the rats were all dead after 24 hours. Among the eight types of SISPH models ( E M) lobus exter2 nalis sinister resected in first time lobus cent ralis sinister and lobus cent ralis resected in second time lobus dex2 ter sinister resected in third time and lobus caudatus sinister resected in forth time and E (3 times of liver resec2 tion interval time between resections was 4 4 and 4 hours respectly in turn) G (3 times of liver resection interval time between resections was 36 4 and 4 hours respectly in turn) K (4 times of liver resection inter2 val time between resections was and 36 hours respectly in turn) and M models (4 times of liver re2 section interval time between resections was and 36 hours respectly in turn) were selected out accord2 ing t he survival rate and interval time to proceed histological analysis. SISPH could affect t he liver on t he histo2 logical changes and among that the changes of hyperplasia of liver and mitosis of chymohepatocytes were like in E model most conspicuous. Almost all t he hepatocytes of remanent liver lobes becames hyperplasia and t he mitotic index was 15 times of the control. The sharp hyperplasia and quick proliferation gave rise to a series of changes of the liver structure like obvious confusion of the structure of liver lobes and narrowing of the liver si2 nusoid. In t he ot her hand t he percentages of binuclear hepatocytes of all t he hepatoctes might also be affected by the SISPH but there were much distinct among the 4 models. The results showed that the amount of the liver once resected might not exceed 85 % of the whole liver and the sustain limit of rat to SISPH is dependent on t he amount of liver resected and t he interval time between successive partial hepatectomy. It was found t hat the models as represented by groups E G K and M can be considered as useful models for studying the change of liver structure the rate of rat survival in SISPH as well as the molecular mechanism of rat liver regeneration. Key words Rat Model Short interval successive partial hepatectomy (SISPH) Liver regeneration Liver tis2 sue structure 3This work was supported by the National Natural Science Foundation of China (No )

8 : XU Cun2Shuan et al. : Effect of t he short interval successive partial hepatectomy on rat survival and hepatic tissue structure Plate E (Structural change of the rat hepatic tissue and hepatocytes after three time successive partial hep2 atectomy in group E rats) 200 a. (Control) b f. ( Tissue and cell structure of the remanent hepatic lobe by SISPH) b. (Lobus centralis sinister and lobus centralis) c d. (Lobus dexter) e f. (Lobus caudatus) CV : (Central vein) HC : ( Hepatic cord) HS : ( Hepatic sinusoid) BC : (Binucleate cells) V : (Vesicle) GSC : ( Group of the small deep2stained cells)