Intact2Chemoreceptor2Based Biosensor : Detection of Ascorbic Acid

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1 , ACTA CHIMICA SINICA Vol 60, 2002 No 9, Ξ Ξ ( ), (AA) AA, mol/ L, 10-6 mol/ L AA Intact2Chemoreceptor2Based Biosensor : Detection of Ascorbic Acid HONG, Li YIN, Yi2Mei ZHENG, Sai2Jing DUAN, Ya2Nan LIN, Xiang2Qin Ξ ( Department of Chemistry, University of Science and Technology of China, Hefei ) Abstract An intact2chemoreceptor2based biosensor based on the gustatory organ, the maxillary palp of Periplaneta americana, is reported, in which the maxillary palp serves as not only the molecular recognition elements but also the biological transducers The binding events between ligand and chemoreceptor induce action potentials (APs) whose frequency correlates directly to the concentration of stimulants The APs were recorded with a three2electrode system and collected by sound card method developed by our group This is the first time that the biosensor is reported to yield quantitative linear dose2response data over the concentration range from to mol/ L The new biosensor possesses some advantages over the biosensor based on isolated receptor : shorter response time ( < 1 s), higher degrees of sensitivity (detect2limitation below 10-6 mol/ L), broader dynamic response range (over four orders of magnitude) and more preferable reproducibility Keywords chemoreceptor, biosensor, Periplaneta americana, maxillary palp, 1 2 ms [1 ] [5 ], Buch [1 3 ] ; Schutz [4 ] C,,, Ξ E2mail : edu cn Received December 17, 2001 ; revised April 8, 2002 ; accepted June 7, 2002 (No KY2216) (No KY1212)

2 1728 Vol 60, 2002 [6 9 ] (4 ), 1 ( 20 m 200 m / ; [10 ] 200 m / ),OS3020 (20 MHz) ( Goldstar), IBM, CD ( HP CD2writer 8230e), XTL ms ( ( ), PCS2500 ) (Sutter) CoolEdit2000, [11 ] : R = R max / [1 + ( K/ c) n ] R, c, K, n [5 - ] ( Intact chemoreceptor) (isolated receptor), 1 Figure 1 Schematic diagram of the experimental setup 2 4,,, [12, ],,, 2600, 5 9, Hz, L2FDQ21 ( ) 20,80 % 90 % : 2 min, 2 1, 4 10 min Sigma,,

3 No 9 : (83 %, N = 30), mv mv, mol/ L , , , , mol/ L 1 7 mv ( ) ( ) (mol/ L) mol/ L Y = X ( Y, X AA ), r = Figure 3 Dose2response of the sensor for ascorbic acid The frequency of spikes was counted for those with amplitude above 1 7 mv 2 (a) (b) Figure 2 Comparison of single2unit ( a) and multi2unit ( b) activities 1 7 mv 1 7 mv, 3 2 ( ),, 5 mmol/ L 4

4 1730 Vol 60, Figure 4 Repetition experiments induced by ascorbic acid (5 mmol/ L) The experiments of n = 1, 2, 3 were on the same palp and the others on different palps ( %), 20, 80 % 90 %, 4 8 h 3 8 h [14 ] ; 8 h [2 ] %, mv, mol/ L 52,,,,,, References, 3 4 ( 1 2 ms), 1 s ms Becker Peters [13 ] 1 Buch, R M ; Rechnitz, G A Anal Chem 1989, 61 (8), 533 ( 2 Buch, R M ; Becker, T Q ; Rechnitz, G A Anal ) 20 kd 40 kd Chim Acta 1991, 243, Wijesuriya, D ; Rechnitz, G A Anal Chim Acta 1992, ( ), 264, 189, 4 Schutz, S ; Weissbecker, B ; hummel, H E Nature

5 No 9 : , 398, Yin, Y 2M Ph D Dissertation, University of Science and Technology of China, Hefei, 2001 (in Chinese) (,,, 2001 ) 6 Castro, S S L ; Balbo, V R ; Barberia, P J S Talanta 2001, 55, Ruiz, M A ; Fernandez, de C M L ; Molina, D A J Pharm Biomed Anal 1999, 20, Liu, Z H ; Wang, Q L ; Mao, L Y Anal Chim Acta 2000, 413, Zhou, Y X ; Nagaoka, T ; Li, F Talanta 1999, 48, Sun, D 2Y ; Guo, Y2L ; Ma, L2G Signal Communication in the Cell, 2nd Edn, Science Press, Beijing, 1998 (in Chinese) (,,,,,,, 1998 ) 11 Maes, F W Chem Senses 1985, 10, Yin, Y 2M ; Lin, X2Q J Univ Sci Technol China 2002, in press (in Chinese) (,,, 2002, ) 13 Becker, A ; Peters, W J Insect Physiol 1989, 35, Christopher, W B ; David, R C ; Garry, A R Anal Chem 1995, 67, 763 (A LI, L T ; DONG, H Z )

1 0 Graphical Abstract Vol 60, No 9 Preparation and Tribological Properties of Ni2 P Electroless Composite Coating Containing Inorganic Fullerene2like WS 2 Nanomaterials CHEN, Wei2Xiang ; TU,

6 1 0 Graphical Abstract Vol 60, No 9 Preparation and Tribological Properties of Ni2 P Electroless Composite Coating Containing Inorganic Fullerene2like WS 2 Nanomaterials CHEN, Wei2Xiang ; TU, Jiang2Ping ; MA, Xiao2 Chun ; XU, Zhu2De ; CHEN, Wen2Lu ; WANG, Jiu2Gen ; CHENG, Dan2Hong ; XIA, Jun2Bao ; GAN, Hai2Yang ; J IN, Ya2Xu ; TENNE, R ; ROSENSTVEIG, R Acta Chimica Sinica 2002, 60 (9), 1722 Ni2P composite coating containing inorganic fullerene2like WS 2 nanosize particles was prepared by electroless codeposition It was found that the Ni2P2( IF2WS 2 ) composite coating exhibited both higher wear resistance and lower friction coeffi2 cient than Ni2P, Ni2P2(layer 2H2WS 2 ) and Ni2P2graphite electroless coating Intact2Chemoreceptor2Based Biosensor : Detec2 tion of Ascorbic Acid HONG, Li ; YIN, Yi2Mei ; ZHENG, Sai2Jing ; DUAN, Ya2Nan ; LIN, Xiang2Qin Acta Chimica Sinica 2002, 60 (9), 1727 A linear dose2response relationship between spikes frequency and the stimulant (ascorbic acid) was found over a wide concentration range on the intact2chemore2 ceptor biosensor based on the maxillary palp of Periplaneta americana Synthesis and Crystal Structure of Tricyclic Azacrown Ether Dicarboxylic Acid Derivative MA, Shu2Lan ; ZHU, Wen2Xiang ; YANG, Lu ; YAN, Xi Acta Chimica Sinica 2002, 60 (9), 1732 A tricyclic azacrown ether dicarboxylic acid derivative has been prepared The crystal structure of the compound was determined by X2ray diffraction method It belongs to monoclinic crystal system, space group P2 1 / c with a = (17) nm, b = (3) nm, c = (19) nm ; = (3) ; Z = 4, V = (4) nm 3, R 1 = , wr 2 = Executive editor PAN, B F ; computer typesetter ZHENG, G C

Fura22. Ca 2 +, Study on Transmembrane Behaviors of Ca 2 + to Escherichia coli Cells with Fura22 Fluorescence Probe

Fura22. Ca 2 +, Study on Transmembrane Behaviors of Ca 2 + to Escherichia coli Cells with Fura22 Fluorescence Probe 2004 62 4, 445 448 ACTA CHIMICA SINICA Vol 62, 2004 No 4, 445 448 Fura22 Ca 2 + Ξ, a, b b b a Ξ ( a 430072) ( b 430072) Fura22/ AM, Ca 2 + HB101, CaCl 2, Ca 2 +, ; Ca 2 +, Ca 2 +, Ca 2 +, Fura22/ AM, Study