Mapping QTL for Seedling Root Traits in Common Wheat

Transcription

1 2005,38(10): Scientia Agricultura Sinica 1,2, / / DH A 4B 2D 6D 7D DH Mapping for Seedling Root Traits in Common Wheat ZHOU Xiao-guo 1,2,3, JING Rui-lian 1, HAO Zhuan-fang 1, CHANG Xiao-ping 1, ZHANG Zheng-bin 2 ( 1 National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm and Biotechenology, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing ; 2 The Research Center of Agricultural Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang ; 3 Graduate School of the Chinese Academy of Sciences, Beijing ) Abstract: A doubled haploid (DH) population with 150 lines, derived from a cross between two common wheat varieties Hanxuan10 and Lumai14, was used in this research. Several root traits including root number (RN), maximum root length (MRL), root fresh weight (RFW), root dry weight (RDW), ratio of root fresh weight to shoot fresh weight (RFW/SFW) and ratio of root dry weight to shoot dry weight (RDW/SDW) per plant of hydroponic seedlings were measured under water stress and control conditions, respectively. Quantitative trait loci () and G E interactions for these traits were detected using mixed-model-based composite interval mapping method. A total of 11 additive and 15 pairs of epistatic associated with root traits were mapped on all chromosomes except 5A, 4B, 2D, 6D and 7D. Among these, 3 with additive effects and 2 pairs of epistatic controlling root number, 3 with additive effects and 3 pairs of epistatic controlling maximum root length, 2 with additive effects and 2 pairs of epistatic controlling root fresh weight, 2 with additive effects and 3 pairs of epistatic controlling root dry weight, 2 pairs of epistatic controlling ratio of root fresh weight to shoot fresh weight and 1 with additive effects and 3 pairs of epistatic controlling ratio of root dry weight to shoot dry weight were detected. One additive environment and three pairs of epistatic environment interaction effects were also identified. The possibility of using marker-assisted selecting root traits and drought tolerance in wheat was discussed. Key words: Wheat; Doubled haploid (DH) population; Root trait; Quantitative trait loci () mapping; G E interaction AA2Z CB xgzhou@mails.gscas.ac.cn Tel/Fax: ; jingrl@caas.net.cn

2 Hoagland 1 1 root number RN maximum root length MRL [1] root fresh weight RFW shoot fresh weight, SFW 100 [2 8] 10 min 80 IRAT109 ratio of root fresh weight to 116 DH shoot fresh weight RFW/SFW ratio of root dry weight to shoot dry weight RDW/SDW 1.3 [9] 2 DH [10] Mapper1.6 [15] LOD>2.5 P <0.05 P <0.005 P< Q+. - [16] 1.1 DH DH 1 DH [11] 14 DH 395 [12] 1.2 DH 12±2 20±4 DH DH ml 2.2 [13] 4 Hoagland Mapper d 3 2 2B 7A 7B -0.5 MPa PEG QRn.cgb-2B Hoagland [14] QRn.cgb-7B

3 % 3.07% 5.43% % 5B 7A 4.66% QRfw.cgb-5B % 1D 2B 6B cm QRdw.cgb-2A 10 1 DH Table 1 Root traits in seedlings of DH population under water stress and control conditions Traits 10 Hanxuan10 14 Lumai14 Water stress Mean 1) DH DH lines Hanxuan10 Lumai14 Range Skew Kurt Control Mean DH DH lines Range Skew Kurt RN ± ± ± ± MRL (cm) ± ± RFW (mg) ± ± RDW (mg) ± ± RFW/SFW ± ± RDW/SDW 1 ± Mean ± standard deviation 2 DH Table 2 Additive and additive environment interaction effects of for root traits of DH population seedlings Trait RN MRL Site 1) 2) 3) 4) 3) LOD Marker interval (cm) A H 2 (A) (%) AE 1 H 2 (AE 1 ) (%) QRn.cgb-2B Xgwm429-Xgwm * 3.56 QRn.cgb-7A WMC301-WMC *** 4.66 QRn.cgb-7B Xgwm302-P * 5.43 QMrl.cgb-1D CWM1-WMC *** 1.40 QMrl.cgb-2B Xgwm319-WMC *** 3.07 QMrl.cgb-6B.2 WMC182-Xgwm RFW QRfw.cgb-5B P P *** 1.42 QRfw.cgb-7A P WMC ** 1.37 RDW QRdw.cgb-2A Xpsp3088-WMC * 2.02 QRdw.cgb-5B Xgwm408-Xgwm * 1.95 RDW/SDW QRdw/sdw-5D Xgdm3-Xgdm * ) LOD ; 2) * ** *** P <0.05 P<0.005 P < ) H 2 A% H 2 AE 1 % 4) E 1 AE 1 E 1 1) Genetic distance between the most likely position of putative and the left flanking marker in marker interval; 2) Positive value indicates the Hanxuan10 genotype having positive effect on the trait. Negative value represents Lumai14 positive effect. *, ** and *** represent the significance level in P <0.05 P <0.005 and P <0.001, respectively: 3) H 2 (A) (%) indicates the variance explained by additive, H 2 (AE 1 ) (%) indicates the variance explained by additive environment interaction; 4) E 1 represents water stress environment; AE 1 represents the effect of environment interaction in water stress environment, the effect of environment interaction in control environment is the reverse of AE 1, but the same value

4 mg QRdw.cgb-5B mg 1.29% QRfw.cgb-1B QRfw.cgb-7B 2.02% 1.95% % % A-3B 3A-4D 3 2 2B-5D 6A-7A 6.04% 10.74% 4.76% 2.25% QRn.cgb-2B QRn.cgb-5D QRdw/sdw-4A.2 QRdw/sdw-7B % QRn.cgb-2B QRdw/sdw-5D QRdw/sdw-6A % 1B-3B 2B-3B 4A-6B QRdw/sdw-4A.1 QRdw/sdw-6A.2 QMrl.cgb-4A QMrl.cgb-6B % % 10.93% 2 QRfw.cgb-4A 1.26% 3.22% 2 QRfw.cgb-5D 5D Xgdm3-Xgdm43-Xgwm174 3 Table 3 Epistasis and epistasis environment interaction effect of s on seedling root traits Trait RN MRL RFW RDW RFW/SFW RDW/SDW 1 Site 1) 2 Site 1) 2) 3) LOD Marker interval (cm) Marker interval (cm) AA H 2 AA (%) AAE H 2 (AAE) (%) QRn.cgb-2B Xgwm429-Xgwm388 0 QRn.cgb-5D Xgdm3-Xgdm ** 4.76 QRn.cgb-6A Xgwm169-WMC QRn.cgb-7A WMC301-WMC ** 2.25 QMrl.cgb-1B P P QMrl.cgb-3B.1 P WMC *** 2.03 QMrl.cgb-2B Xpsp3404-Xgwm630 0 QMrl.cgb-3B.2 P P *** 1.99 QMrl.cgb-4A WMC468-P QMrl.cgb-6B.1 P P ** 2.46 QRfw.cgb-1B Xpsp3027-Xgwm164 0 QRfw.cgb-7B CWM467-CWM * 0.58 QRfw.cgb-4A Xgwm601-Xgwm610 0 QRfw.cgb-5D Xgdm43-Xgwm * ** 1.29 QRdw.cgb-1A.1 P P QRdw.cgb-3A P CWM * 3.22 QRdw.cgb-1A WMC120-Xgwm135 0 QRdw.cgb-3D Xgdm8-Xgwm QRdw.cgb-7B.1 Xgwm400-P QRdw.cgb-7B.2 Xgwm68.1-WMC * 1.86 QRfw/sfw-3A.1 WMC532-Xgwm QRfw/sfw-3B Xgwm299-CWM * 6.04 QRfw/sfw-3A.2 WMC21-WMC QRfw/sfw-4D Xgwm192-WMC * QRdw/sdw-4A.1 Xgwm265-WMC161 0 QRdw/sdw-6A.2 Xgwm169-WMC * ** QRdw/sdw-4A.2 P P QRdw/sdw-7B WMC269.4-P * 5.46 QRdw/sdw-5D Xgdm3-Xgdm43 2 QRdw/sdw-6A.1 Xgwn334-WMC * ) LOD ; 2) > * ** *** P <0.05 P <0.005 P <0.001; 3) AAE 1) Genetic distance between the most likely position of putative and the left flanking marker in marker interval; 2) Direction of epistasis effect: positive value represents parent type > recombinant type; negative value represents recombinant type > parent type *, ** and *** represent the significance level in P <0.05, P<0.005, P <0.001, respectively; 3) AAE is the effect of environment interaction in water stress environment. The effect under control condition is the reverse of AAE

5 QRn.cgb-5D 1B 2A 5A 2 QRfw.cgb-5D QRdw/sdw-5D 3 1B-2B 2A-2A 3B-6B 6A-6A 4 QRfw.cgb-5D QRfw.cgb-4A 1A 2 2A 2 6A 3 7 1A 2A 2B 2 QRdw/sdw-5D 4A 5B 6D 7 2A-6D 6A Xgwm169-WMC417.1 QRn.cgb-6A QRdw/sdw-6A.2 5A-5D 1 5D [21] Xgdm3-Xgdm43-Xgwm174 6A Xgwm169- QRdw.cgb-2A RIL WMC [22] RIL W7984 Triticum tauschii DD Altar84 AABB [17 19] Opata85 DH [18] QRdw.cgb-7B.1 DH 7B Xgwm400-P [12] QRfw.cgb-7A [20] 7A WMC9- P WMC422 [12] [9] DH DH A 5B 2 DH 5A 4B 2D 6D 7D % 10.74% DH 4.1 DH Hoagland 5A 4B 2D 6D 7D 16 W7984 Opata

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