Effect of temperature on growth and sex differentiation at early developmental stage of redfin puffer ( Takifugu rubripes)

Transcription

1 South China Fisheries Science Vol. 10, No. 5 Oct., 2014 doi: / j. issn , ,,, ( 1., ; 2., ; 3., ; 4., ) : ( Takifugu rubripes) 4 ( ),, ( ), , 70, ; 80 90, , % %, % %, % %, % %, 15 : ; ; ; ; : S : A : ( 2014) Effect of temperature on growth and sex differentiation at early developmental stage of redfin puffer ( Takifugu rubripes) LIU Yongxin 1, ZHOU Qin 2, ZHANG Hongtao 2, JIANG Changbo 3, ZHANG Fuchong 4 ( 1. Chinese Academy of Fishery Sciences, Beijing , China; 2. Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao , China; 3. Tianhe Aquaculture Farming Ltd. Co., Tangshan , China; 4. Hebei Ocean and Fisheries Science Research Institute, Qinhuangdao , China) Abstract: To analyze the effect of water temperature on growth trait and sex differentiation of redfin puffer ( Takifugu rubripes), we reared a full-sib family at four temperatures of and normal temperature ( ). The results showed that the growth rate of the fish decreased with decreasing cultivation temperature. The fingerlings in normal temperature group presented the highest growth rate, followed by and 15 in descending order. For fish at dph, different temperature groups had similar variation trends in absolute growth ratio and growth index. For fish before 70 dph and those at dph, the normal temperature group and 19 group presented the highest absolute growth ratio and growth index, respectively. We used morphological examination and histological section to identify the ratio of males and females for fish at180 dph. The percentages of males and females were % and % in 15 group, % and % in 17 group, % and % in 19 group as well as % and % in normal temperature group. In conclusion, low temperature produced effectively higher proportions of males and 15 group was the optimum treatment group. Key words: temperature; redfin puffer; growth; sex differentiation; family : ; : : ( 2013A0505) ; ( 2012BAD26B01) : ( ),,, liuyx@ cafs. ac. cn : ( ),, laozhou_529@ hotmail. com

2 5 : 25 ( Takifugu rubripes),,,,,,,,,,, ( tetrodonine) ( tetrodonic acid) ( tetrodotoxin) 1 ( hepatoxin),, 1. 1 [ 1] [ 2 ] [ 3 ] [ 4 ], 5 14, 3 d,,, 1,, , 3), ( [ 5] ) 3, 300, ( Oreochromis niloticus) [ 6 ], ( Hexagrammos otaki) [ 7 ] ( Lates cal- carifer) [ 8 ] ( Cirrhinus mrigala) [ 9] ( Rhamdia quelen) [ 10 ] ( Oncorhynchus mykiss) [ 11 ] ( Horabagrus brachysoma) [ 12] ( aralichthys lethostigma) [ 13 ] 40, 7, cm ;, ( 200 L L - 1 ),,, Motic Images lus 3. 2, 90,, ( Cynoglossus semilaevis) [ 14 ] ( Cyprinus carpio) [ 15 ] ( Salmo gairdneri) [ 16] ( O. nerka) [ 17 ] ( Dicentrarchus labrax) [ 18 ] ) 10, ( O. mossambicus) [ 1 9] (. olivaceus) [ 20] 20 : ( d, 180, ( ) ( h 70% [ 21 ] 2 ( Odontesthes argentinensis atagonina hatcheri) [ 2 2] H. E., 2, ( Olympus E-300),

3 Duncan s ( SAS 8. 2 ) 2 7,,, 1 l2 - l : = = t 2 - t 1, 4 ( logl 2 - logl 1 ) / l 1 l 1 l 2, ( < 0. 05, 1) t 1 t 2 Excel , 2 ;, ; 30 70, 2 ;, 1, 2 3 ; 80 90, ( ) Tab. 1 Comparison of body length of fingerlings at early developmental stage in different groups ( X SD) cm treatment group dph Group a a a a a a a 2 Group b b b b b b b 3 Group c c c c c c c control d d d d d d d : ( < 0. 05) Note: In the same column, values with different letter superscripts mean significant difference ( <0. 05). Fig. 1 1 ( ( b) a) Trend of absolute growth rate ( a) and growth index ( b) along with dph for fingerlings at early developmental stage in different groups 2. 2 A B, ; 2 - b, a, ( ),, ( ),,

4 5 : 27 2 a, ; b B, A Fig. 2 Histological section of gonad Black and white arrows represent primary oocyte and oogonium in Fig. 2 -a, arrows represent Type A spermatogonia and Type B spermatogonia in Fig. 2-b, respectively. Black and white respectively., 2 ( 2) 1 ( 26 ), ( 4 ) ; % % % % % ( 21 ), ( 9 ) ; ( <0. 05), % % % 2 Tab. 2 Composition of female and male in different groups treatment group 1 Group 1 2 Group 2 3 Group 3 control / number of individuals /% percentage ( O. niloticus O. aureus) [ 23 ] ( Scoph- thalmus maximus) [ 24 ] ( Epinephelus co- ioides) [ 25 ] : 1), [ 14] [ 23 ] ;, [ 2 5] ph,, ;,,,,

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