South China Fisheries Science , ; g ( Monopterus albus) 2 400, 5, kg g ( GOT) ( GPT) ( P > 0. 05), g kg - 1,

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1 South China Fisheries Science Vol. 9, No. 2 Apr., 203 doi: / j. issn , 2,,,, (., 48000; 2., 4028) : 25 g ( Monopterus albus) 2 400, 5,, 4 0 gkg - 20kg g - 30kg g - 40 gkg - ( Vitis amurensis) ( Artemisia annua) ( 4 ), d, ( GOT) ( GPT) ( TP), ( GLU) ( TG), 20 gkg - 30 gkg - ( P < 0. 05) ; 0 g kg - 40 gkg - ( P > 0. 05) 20kg g - 30 gkg - ( P > 0. 05), g kg -, : ; ; ; ; : S ; S : A : ( 203) Effects of plant extract from grape seed and sweet wormwood on intestinal digestive enzyme activities and blood biochemical parameters of eel ( Monopterus albus) HUANG Guangzhong, HU Hui, XIAO Keyu 2, LUO Shimin, XIE Peirong, OUYANG Juying (. Huaihua Vocational and Technical College, Huaihua 48000, China; 2. Hunan Agricultural University, Changsha 4028, China) Abstract: To explore the growth promotion mechanism of plant extract from grape ( Vitis amurensis) seed and sweet wormwood on Monopterus albus, healthy eel fingerlings were randomly divided into five groups. We fed with basal feed, and four experimental groups with diets containing respectively 0 gkg -, 20 gkg -, 30 gkg -, 40 g kg - mixture, which were extracted from grape seed and from sweet wormwood( Artemisia annua) with a four-to-one proportion. We determined the digestive enzyme activities and blood biochemical parameters of five eel groups after feeding for 00 d in Results show that basal diets with the mixture could improve digestive enzyme, blood glutamic-oxaloacetic transaminase ( GOT) and glutamic-pyruvic transaminase ( GPT) s activities, and triglyceride ( TG) s contents of eel. Compared with the, and total protein ( TP) s content; and could reduce blood glucose ( GLU) the above indexes had significant difference ( P < 0. 05) when the dosage of the plant extract was 20 gkg - or 30 gkg -, while the above indexes had no significant difference ( P > 0. 05) when it was 0 gkg - or 40 gkg -. There was no significant difference between 20 gkg - and 30 gkg - experimental groups. In conclusion, extract from grape : ; : : ( 202NK3056) : ( ),,,, hzhgzh@ 26. com

2 2 : 7 seed and sweet wormwood with a four-to-one ratio, when mixed in diets with dosage gkg -, could increase digestive enzyme activities, regulate blood biochemical parameters, enhance the digestion and metabolic activity of 3 main nutritive materials and improve the growth of fish. Key words: plant extract; grape seed; Monopterus albus; digestive enzyme; blood biochemical parameter, ( ) ;, (, ], ) 2], 3], 4], 5] 6] 7] 8 ]. 4, ( Vitis amuren- sis) ( Artemisia annua), 90%, 20 30, cm 7 d 00 gm - 2,, ; ( Monopterus albus), 30 gl min,, 2 ) : 3 d, 3 d,,,. 2) : ( ) g 2 400, 5. 2,. 6 m;, 5 :, 9: 00, 4 6, , ph , 2. 0 m m2. 0 m,. 5., 8 0 g kg - 20 kg g - 30kg g - 40 gkg -, 2. 5 ml, ( ), ( 2), 4 8 h, rmin - 0 min, ( ) ;, 2,,, - 80, 24 h 4, d 8: 00 Tab. Experimental design / gkg - experimental group / g - initial weight / number / gkg - dosage of extract 0

3 72 9 ingredient 2 Tab. 2 Ingredients and nutritional level in basal diet w/ % content nutritional level w/ % content soybean meal / MJ kg - total energy inferior flour 6. 0 crude protein rapeseed meal 4. 0 crude lipid corn protein powder 5. 0 ash wheat bran 5. 0 calcium Peruvian fish meal 0. 0 total phosphorus. 2 cottonseed meal 8. 0 available phosphorus 0. 8 rapeseed oil 3. 0 lysine. 89 talc. 4 glutamic acid 5. 5 calcium dihydrogen phosphate. 5 adhesive 0. 5 choline 0. 3 salt 0. 3 premix. 0 : : B 0 mgkg -, B 2 20 mg kg -, B 6 30 mgkg -, B mgkg -, 5 mgkg -, 50 mgkg -, 00 mgkg -, 00 mg kg -, K 0 mg kg -, D IUkg -, kg -, 60 mgkg -,. 0 mgkg -, 0. 3 mg kg mgkg -, C 00 mgkg -, A IU kg -, E 00 mgkg -, 250 mgkg -, 80 mg kg -, 2 mgkg -, 20 mg Note: remix ingredient: vitamine B 0 mgkg -, vitamine B 2 20 mg kg -, vitamine B 6 30 mgkg -, vitamine B mgkg -, folic acid 5 mg kg -, calcium pantothenate 50 mgkg -, myoinositol 00 mgkg -, niacin 00 mgkg -, biltin 0. 5 mgkg -, vitamine C 00 mg kg -, vitamine A IUkg -, vitamine K 0 mgkg -, vitamine D IUkg -, vitamine E 00 mgkg -, magnesium 250 mg kg -, iron 80 mg kg -, copper 2 mgkg -, manganese 20 mgkg -, zinc 60 mgkg -, iodine. 0 mg kg -, selenium 0. 3 mg kg ),, -,, : = ( W t - W 0 ) / W 0 00; = ( lnw t - lnw 0 ) / t] 00; = F/ ( W t + W d - W 0 ) ; = ( W t - W 0 ) /( F P) 00; = / ; ( TG) TCH) 00, W t ( g) ; W 0 ( g) ; W d ( g) ; P. 6 ( % ) ; F ( g) ; t ( d) ( X SD),, = %, rmin - 0 min, ( ( GOT) ( GPT), ( TP), ; ( GLU) -, SPSS 7. 0, Duncan s, P C d

4 2 : 73 ( 3), 20 gkg - 30 g kg - ( P < 0. 05), 0 gkg - ( 4) 3, 40 gkg - ( P > 0. 05) 20 gkg - 30 gkg -,, 20 gkg - 30 gkg - 00% %, ( P < 0. 05), 0 g kg - 40 gkg - ( P % %, 70% > 0. 05), ( P > 0. 05) 4 ( P > 0. 05) kg -, 2. 2 ( P > 0. 05), %, ( P < 0. 05) ; 40 g ( P > 0. 05) 20kg g - 30 gkg - 3 Tab. 3 Effect of plant extract on growth performance of M. albus / gkg - experimental group /g - first weight /g - last weight a ab b b ab /% weight gain rate a ab b b ab /% protein efficiency ratio a ab b b ab /% d - specific growth rate a ab b b ab feed coefficient a ab b b ab /% survival ratio : ( P >0. 05), ( P C 0. 05), Note: alues with same superscripts within the same row have no significant difference( P > ), and those with different superscripts were significantly different from one another( P < ). The same case in the following tables. 4 Tab. 4 Effect of plant extract on digestive enzyme activities of M. albus / g kg - experimental group / U. mg - amylase a a b b ab / U. g - lipase a a b b ab / U. mg - trypsin a a b b a 2. 3 GOTGPT TCH, TP,, GLU 3. 76% 38. 0%, ( 5), GOT 20 g kg - 30 gkg - GLU % 30. 0% 68. 4%, 30 g kg - GOT 38. 0%, ( P < 0. 05), 0 g kg - 40 kg g - ( 68. 4% ), ( P < 0. 05) ; GPT 3. 63% %, 30 g kg - GPT %, 20 g kg - 30 gkg - TG ( % ), ( P < 0. 05) ; TP 7. 5% 4. 4%, 20 gkg - TP 4. 4%, ( P < 0. 05), GLUTG ( P > 0. 05) ; TG. 8% % %, ( P < 0. 05), ( P >0. 05) ; TCH 3. 73% 7. 4%, ( P >0. 05)

5 Tab. 5 Effect of plant extract on blood biochemical parameters of M. albus / gkg - experimental group / IUL - GOT a ab b b b / IUL - GPT a ab b b ab ( ) / gl - TP a a b ab a c( ) / mmoll - GLU a ab b b ab c( ) / mmoll - TG a ab b b ab c( ) / mmoll - TCH ,, ( 3), 6] ( 5), 4,, 20 g kg - 30 g kg - GOTGPT TP 68. 4% % 4. 4%,, GOTGPT TP 3. 2, 20 gkg - GPTTP, 30 gkg - GOT, 40 gkg - GOT GPT TP 9] ( 4),,, gkg % GLU % %,, 7 ], 8],,, GLU,, 30 gkg - ( 38. 0% ), 20 g kg - 30 gkg -, 40 gkg - 20 gkg - 30 gkg -, 30 gkg -, 0] TG ] 2],, TCH, 9] 20],, TCHTG gkg -, 20 gkg - 30 g kg - TG, % %,, 3 ], TGTCH, 6. 53%, TG, TCH, 3. 3, ; GPTGOTTPGLUTG TCH,,, 4] GPTGOT TP 5] TP, GOT GPT, 20], TGTCH GOT GPT 2],,

6 2 : 75 TCH, J]., 0],,,. J]., 2005, 25( 6) : 29-3., ],,,.,, GPT J]., 2008, 27( 9) : GOTTP, GLUTGTCH, 2],,,. 5, J]., 2007, 35 ( 26 ) : gkg - 3],,,. : ],,. J]., 2009, J]., 200 ( 5 ) : ],,,., 200, 28( ) : J]., 2008, 20 ( 5 ) : ],,. 4( 5) : J]., 2006, 30( ) : ],. J]. :, 2002, 4 J]., 2002, 22( 4) : ],,,. 4 8]. J]. J]., 2008, 27( 2 ) : ],,,. 8 9],,,. 5 J]., 2008, 28( 5) : ],,. 20],. J]., 2006 ( ) : ],,,. 9 2],,,. J]., 200 ( 0 ) : ],,,. EA-II BA-I, 993, 8( ) : J]., 200, 3( 4) : ],,,. - 39( 3) : ],. J]. 6],,,. J]., 2007, 7],,,. ( 3) : , 2008, ( 6) : J]., 2003, 35 ( ) : 8-0. J]., 2003, 4 ( 3 ) : J]., 202, 8 ( 2) :