ENCODE DCC Antibody Validation Document
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1 ENCODE DCC Antibody Validation Document Date of Submission Name: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description: Species Target Species Host Validation Method #1 Validation Method #2 Purification Method Polyclonal/ Monoclonal Vendor URL: Reference (PI/ Publication Information) Please complete the following for antibodies to histone modifications: if your specifications are not listed in the drop-down box, please write-in the appropriate information Histone Name AA modified AA Position Modification
2 Validation #1 Analysis Insert Validation Image (click here)
3 Validation #2 Analysis Insert Validation Image (Click here)
4 34 entries (13 single hits) retrieved from /home/tpp/tpp/ _bowling/int-gb_ _ltq_bowling_cebpb-sequest.prot.xml * corresponds to peptide is_nondegenerate_evidence flag 1 UniRef100_B4DP56 UniRef100_P12277 UniRef100_UPI0001AE69A % spectra: 5 id's: 1.16% >cdna FLJ52237, highly similar to Creatine kinase B-type (EC ) n=1 Tax=Homo sapiens RepID=B4DP56_HUMAN >Creatine kinase B-type n=1 Tax=Homo sapiens RepID=KCRB_HUMAN >UPI0001AE69A0 related cluster n=1 Tax=Homo sapiens RepID=UPI0001AE69A0 Length: 346aa 2 UniRef100_P % 0 spectra: 23 id's: 5.32% >Keratin, type I cytoskeletal 9 n=1 Tax=Homo sapiens RepID=K1C9_HUMAN Length: 623aa 3 UniRef100_P % peps: 6 4 id's: 3.26% >Elongation factor Tu, mitochondrial n=1 Tax=Homo sapiens RepID=EFTU_HUMAN Length: 452aa 4 UniRef100_Q9N0W4 UniRef100_Q9N0W % id's: 1.40% spectra: 6 >Anti-human A33 heavy chain domain (Fragment) n=1 Tax=Oryctolagus cuniculus RepID=Q9N0W4_RABIT >Anti-human A33 heavy chain domain (Fragment) n=1 Tax=Oryctolagus cuniculus RepID=Q9N0W6_RABIT Length: 124aa 10a UniRef100_B2RA03 UniRef100_P % peps: 8 6 id's: 3.49% >cdna, FLJ94640, highly similar to Homo sapiens keratin 18 (KRT18), mrna n=1 Tax=Homo sapiens RepID=B2RA03_HUMAN >Keratin, type I cytoskeletal 18 n=1 Tax=Homo sapiens RepID=K1C18_HUMAN Length: 430aa 10b UniRef100_P13645 UniRef100_UPI00017BCE7F % id's: 0.86% >Keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=K1C10_HUMAN >keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=UPI00017BCE7F Length: 584aa 11a UniRef100_B3KM39 UniRef100_Q9H0C % 8 id's: 9.76% spectra: 43 >cdna FLJ10181 fis, clone HEMBA , highly similar to Homo sapiens integrin-linked kinase-associated serine/threonine phosphatase 2C (ILKAP), transcript variant 1, mrna n=1 Tax=Homo sapiens RepID=B3KM39_HUMAN >Integrin-linked kinase-associated serine/threonine phosphatase 2C n=1 Tax=Homo sapiens RepID=ILKAP_HUMAN Length: 392aa 12a UniRef100_B4DTG2 UniRef100_P % spectra: 5 id's: 1.16% entries: 1 >cdna FLJ56389, highly similar to Elongation factor 1-gamma n=1 Tax=Homo sapiens RepID=B4DTG2_HUMAN >Elongation factor 1-gamma n=2 Tax=Homo sapiens RepID=EF1G_HUMAN Length: 487aa 13a UniRef100_C5IWV5 UniRef100_P % 4 spectra: 58 >Trypsinogen n=1 Tax=Sus scrofa RepID=C5IWV5_PIG >Trypsin n=1 Tax=Sus scrofa RepID=TRYP_PIG id's: 8.88% entries: 2 Length: 246aa 14a UniRef100_O14929 UniRef100_Q6P % id's: 0.46% spectra: 2 >Histone acetyltransferase type B catalytic subunit n=2 Tax=Homininae RepID=HAT1_HUMAN >Histone acetyltransferase 1 n=1 Tax=Homo sapiens RepID=Q6P594_HUMAN Length: 419aa 15a UniRef100_P02769 UniRef100_UPI000179EC85 00
5 9.2% peps: 6 4 id's: 3.25% entries: 3 >Serum albumin n=1 Tax=Bos taurus RepID=ALBU_BOVIN >Serum albumin precursor (Allergen Bos d 6) (BSA). n=1 Tax=Bos taurus RepID=UPI000179EC85 Length: 607aa 16a UniRef100_P % 6 8 id's: 8.77% >Keratin, type II cytoskeletal 1 n=1 Tax=Homo sapiens RepID=K2C1_HUMAN entries: 3 Length: 644aa 17a UniRef100_P05787 UniRef100_Q7L4M3 UniRef100_Q969I % >Keratin, type II cytoskeletal 8 n=1 Tax=Homo sapiens RepID=K2C8_HUMAN >KRT8 protein n=1 Tax=Homo sapiens RepID=Q7L4M3_HUMAN >KRT8 protein (Fragment) n=1 Tax=Homo sapiens RepID=Q969I0_HUMAN Length: 483aa 18a UniRef100_P06576 UniRef100_Q0QEN % peps: 4 spectra: 9 id's: 2.07% >ATP synthase subunit beta, mitochondrial n=1 Tax=Homo sapiens RepID=ATPB_HUMAN >ATP synthase subunit beta (Fragment) n=1 Tax=Homo sapiens RepID=Q0QEN7_HUMAN Length: 529aa 19a UniRef100_P17676 UniRef100_Q9BSC % id's: 0.69% >CCAAT/enhancer-binding protein beta n=1 Tax=Homo sapiens RepID=CEBPB_HUMAN >CEBPB protein (Fragment) n=2 Tax=Eutheria RepID=Q9BSC0_HUMAN Length: 345aa 20a UniRef100_P % 4 7 >52 kda Ro protein n=1 Tax=Homo sapiens RepID=RO52_HUMAN id's: 8.48% entries: 1 Length: 475aa 21a UniRef100_Q562R % peps: 5 2 id's: 2.55% >Beta-actin-like protein 2 n=1 Tax=Homo sapiens RepID=ACTBL_HUMAN Length: 376aa 22 UniRef100_P % peps: 4 1 id's: 0.78% >Anionic trypsin n=2 Tax=Canis lupus familiaris RepID=TRY2_CANFA Length: 247aa 23 UniRef100_A6NK07 UniRef100_B5BU01 UniRef100_P20042 UniRef100_Q4R5G5 UniRef100_Q96I % spectra: 2 id's: 0.47% >Eukaryotic translation initiation factor 2 subunit 2-like protein n=1 Tax=Homo sapiens RepID=IF2BL_HUMAN >Eukaryotic translation initiation factor 2 beta n=1 Tax=Homo sapiens RepID=B5BU01_HUMAN >Eukaryotic translation initiation factor 2 subunit 2 n=2 Tax=Homo sapiens RepID=IF2B_HUMAN >Brain cdna, clone: QnpA-11816, similar to human eukaryotic translation initiation factor 2, subunit 2beta, 38kDa (EIF2S2), n=1 Tax=Macaca fascicularis RepID=Q4R5G5_MACFA >EIF2S2 protein n=1 Tax=Homo sapiens RepID=Q96I16_HUMAN Length: 327aa 24 UniRef100_B4DEW9 UniRef100_B4DMT5 UniRef100_O % >cdna FLJ52478, highly similar to Eukaryotic translation initiation factor 3 subunit 5 n=1 Tax=Homo sapiens RepID=B4DEW9_HUMAN >cdna FLJ52696, highly similar to Eukaryotic translation initiation factor 3 subunit 5 n=1 Tax=Homo sapiens RepID=B4DMT5_HUMAN >Eukaryotic translation initiation factor 3 subunit F n=2 Tax=Homo sapiens RepID=EIF3F_HUMAN Length: 208aa 25 UniRef100_B4DL86 UniRef100_B4E2U0 UniRef100_P % >6-phosphogluconate dehydrogenase, decarboxylating n=1 Tax=Homo sapiens RepID=B4DL86_HUMAN >6-phosphogluconate dehydrogenase, decarboxylating n=1 Tax=Homo sapiens RepID=B4E2U0_HUMAN >6-phosphogluconate dehydrogenase, decarboxylating n=3 Tax=Homo sapiens RepID=6PGD_HUMAN Length: 429aa
6 26 UniRef100_P07954 UniRef100_P % >Fumarate hydratase, mitochondrial n=2 Tax=Homo sapiens RepID=FUMH_HUMAN >Isoform Cytoplasmic of Fumarate hydratase, mitochondrial n=1 Tax=Homo sapiens RepID=P Length: 510aa 27 UniRef100_P22695 UniRef100_Q4R4W % >Cytochrome b-c1 complex subunit 2, mitochondrial n=1 Tax=Homo sapiens RepID=QCR2_HUMAN >Brain cdna, clone: QtrA-12443, similar to human ubiquinol-cytochrome c reductase core protein II(UQCRC2), n=1 Tax=Macaca fascicularis RepID=Q4R4W6_MACFA Length: 453aa 28 UniRef100_P % >Heterogeneous nuclear ribonucleoprotein F, N-terminally processed n=1 Tax=Homo sapiens RepID=HNRPF_HUMAN Length: 415aa 29 UniRef100_Q13268 UniRef100_Q UniRef100_Q59F % spectra: 2 id's: 0.47% >Dehydrogenase/reductase SDR family member 2 n=3 Tax=Homo sapiens RepID=DHRS2_HUMAN >Isoform 2 of Dehydrogenase/reductase SDR family member 2 n=1 Tax=Homo sapiens RepID=Q >Dehydrogenase/reductase (SDR family) member 2 isoform 2 variant (Fragment) n=1 Tax=Homo sapiens RepID=Q59F08_HUMAN Length: 258aa 30 UniRef100_B4DTC3 UniRef100_B4E0W4 UniRef100_B9ZVU1 UniRef100_D6RAF8 UniRef100_D6RF44 UniRef100_Q12771 UniRef100_Q14103 UniRef100_Q UniRef100_Q UniRef100_Q UniRef100_UPI0001D3B53A % >cdna FLJ54150, highly similar to Heterogeneous nuclear ribonucleoprotein D0 n=2 Tax=Eutheria RepID=B4DTC3_HUMAN >cdna FLJ61020, highly similar to Heterogeneous nuclear ribonucleoprotein D0 n=1 Tax=Homo sapiens RepID=B4E0W4_HUMAN >Putative uncharacterized protein HNRNPD n=1 Tax=Homo sapiens RepID=B9ZVU1_HUMAN >Putative uncharacterized protein HNRNPD n=3 Tax=Catarrhini RepID=D6RAF8_HUMAN >Putative uncharacterized protein HNRNPD n=2 Tax=Homo sapiens RepID=D6RF44_HUMAN >P37 AUF1 n=1 Tax=Homo sapiens RepID=Q12771_HUMAN >Heterogeneous nuclear ribonucleoprotein D0 n=1 Tax=Homo sapiens RepID=HNRPD_HUMAN >Isoform 2 of Heterogeneous nuclear ribonucleoprotein D0 n=1 Tax=Homo sapiens RepID=Q >Isoform 3 of Heterogeneous nuclear ribonucleoprotein D0 n=1 Tax=Homo sapiens RepID=Q >Isoform 4 of Heterogeneous nuclear ribonucleoprotein D0 n=1 Tax=Homo sapiens RepID=Q >UPI0001D3B53A related cluster n=1 Tax=Homo sapiens RepID=UPI0001D3B53A Length: 303aa 31 UniRef100_P % >Alpha-enolase n=1 Tax=Homo sapiens RepID=ENOA_HUMAN Length: 434aa 32 UniRef100_P % >Adenosylhomocysteinase n=2 Tax=Homo sapiens RepID=SAHH_HUMAN Length: 432aa 10c UniRef100_B4DE59 UniRef100_C9JM50 UniRef100_P % id's: 0.63% entries: 2 >cdna FLJ60424, highly similar to Junction plakoglobin n=1 Tax=Homo sapiens RepID=B4DE59_HUMAN >Putative uncharacterized protein KRT19 n=1 Tax=Homo sapiens RepID=C9JM50_HUMAN >Keratin, type I cytoskeletal 19 n=1 Tax=Homo sapiens RepID=K1C19_HUMAN Length: 563aa 33 UniRef100_D3DTL2 UniRef100_D3DTL4 UniRef100_P13929 UniRef100_P UniRef100_P UniRef100_Q9NPL4 UniRef100_UPI0001AE669E % >Enolase n=1 Tax=Homo sapiens RepID=D3DTL2_HUMAN >Enolase n=1 Tax=Homo sapiens RepID=D3DTL4_HUMAN >Beta-enolase n=1 Tax=Homo sapiens RepID=ENOB_HUMAN >Isoform 2 of Beta-enolase n=1 Tax=Homo sapiens RepID=P >Isoform 3 of Beta-enolase n=1 Tax=Homo sapiens RepID=P >Enolase (Fragment) n=1 Tax=Homo sapiens RepID=Q9NPL4_HUMAN >UPI0001AE669E related cluster n=1 Tax=Homo sapiens RepID=UPI0001AE669E Length: 434aa 34 UniRef100_UPI UniRef100_UPI00017BDB
7 3.3% 0.21 >ANTIBODY n=1 Tax=Homo sapiens RepID=UPI >FabOX117 Light Chain Fragment n=1 Tax=Homo sapiens RepID=UPI00017BDB42 Length: 214aa
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
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