ENCODE DCC Antibody Validation Document
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1 ENCODE DCC Antibody Validation Document Date of Submission Name: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description: Species Target Species Host Validation Method #1 Validation Method #2 Purification Method Polyclonal/ Monoclonal Vendor URL: Reference (PI/ Publication Information) Please complete the following for antibodies to histone modifications: if your specifications are not listed in the drop-down box, please write-in the appropriate information Histone Name AA modified AA Position Modification
2 Validation #1 Analysis Insert Validation Image (click here)
3 Validation #2 Analysis Insert Validation Image (Click here)
4 MS results for lower band (~120 kda): 1 UniRef100_C5IWV5 UniRef100_P confidence: 1.00 max coverage: 30.3% num unique peps: 20 tot indep spectra: 51 share of spectrum id's: 14.90% >Trypsinogen n=1 Tax=Sus scrofa RepID=C5IWV5_PIG Length: 246aa >Trypsin n=1 Tax=Sus scrofa RepID=TRYP_PIG 2 UniRef100_P confidence: 1.00 coverage: 21.1% num unique peps: 12 tot indep spectra: 30 share of spectrum id's: 14.79% subsumed entries: 1 >Keratin, type II cytoskeletal 1 n=1 Tax=Homo sapiens RepID=K2C1_HUMAN Length: 644aa 3 UniRef100_P confidence: 1.00 coverage: 20.6% num unique peps: 12 tot indep spectra: 23 share of spectrum id's: 11.85% subsumed entries: 1 >Serum albumin n=1 Tax=Bos taurus RepID=ALBU_BOVIN Length: 607aa 4 UniRef100_P13645 UniRef100_UPI00017BCE7F confidence: 1.00 max coverage: 20.5% num unique peps: 10 tot indep spectra: 25 share of spectrum id's: 12.86% subsumed entries: 1 >Keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=K1C10_HUMAN Length: 584aa >keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=UPI00017BCE7F 5 UniRef100_P confidence: 1.00 coverage: 20.3% num unique peps: 7 tot indep spectra: 9 share of spectrum id's: 6.42% subsumed entries: 2
5 >Keratin, type II cytoskeletal 2 epidermal n=1 Tax=Homo sapiens RepID=K22E_HUMAN Length: 639aa 6 UniRef100_B4DVQ0 UniRef100_B4DW52 UniRef100_B4E335 UniRef100_B4E3A4 UniRef100_P60709 UniRef100_P63261 UniRef100_Q4R4H6 UniRef100_Q53G76 UniRef100_Q53G99 UniRef100_Q53GK6 UniRef100_Q8WVW5 UniRef100_UPI000186E confidence: 1.00 max coverage: 19.5% num unique peps: 5 tot indep spectra: 6 share of spectrum id's: 2.94% subsumed entries: 4 >cdna FLJ58286, highly similar to Actin, cytoplasmic 2 n=1 Tax=Homo sapiens RepID=B4DVQ0_HUMAN Length: 333aa >cdna FLJ55253, highly similar to Actin, cytoplasmic 1 n=1 Tax=Homo sapiens RepID=B4DW52_HUMAN >cdna FLJ52842, highly similar to Actin, cytoplasmic 1 n=1 Tax=Homo sapiens RepID=B4E335_HUMAN >cdna FLJ57283, highly similar to Actin, cytoplasmic 2 n=1 Tax=Homo sapiens RepID=B4E3A4_HUMAN >Actin, cytoplasmic 1, N terminally processed n=24 Tax=Amniota RepID=ACTB_HUMAN >Actin, cytoplasmic 2, N terminally processed n=9 Tax=Tetrapoda RepID=ACTG_HUMAN >Brain cdna, clone: QflA 11253, similar to human actin, gamma 1 (ACTG1), n=1 Tax=Macaca fascicularis RepID=Q4R4H6_MACFA >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G76_HUMAN >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G99_HUMAN >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53GK6_HUMAN >Putative uncharacterized protein (Fragment) n=1 Tax=Homo sapiens RepID=Q8WVW5_HUMAN >Actin 5C n=1 Tax=Pediculus humanus corporis RepID=UPI000186E470 7 UniRef100_B4DWU0 UniRef100_B4E2J5 UniRef100_P
6 confidence: 1.00 max coverage: 19.3% num unique peps: 2 tot indep spectra: 4 share of spectrum id's: 2.11% subsumed entries: 1 >cdna FLJ56791, highly similar to Keratin, type I cytoskeletal 16 n=1 Tax=Homo sapiens RepID=B4DWU0_HUMAN Length: 135aa >cdna FLJ53570, highly similar to Keratin, type I cytoskeletal 16 n=1 Tax=Homo sapiens RepID=B4E2J5_HUMAN >Keratin, type I cytoskeletal 16 n=1 Tax=Homo sapiens RepID=K1C16_HUMAN 8 UniRef100_P confidence: 1.00 coverage: 17.3% num unique peps: 6 tot indep spectra: 12 share of spectrum id's: 6.20% >Keratin, type I cytoskeletal 9 n=1 Tax=Homo sapiens RepID=K1C9_HUMAN Length: 623aa 9 UniRef100_A5JHP3 UniRef100_P confidence: 0.37 max coverage: 10.0% num unique peps: 1 tot indep spectra: 3 share of spectrum id's: 1.56% >Dermcidin isoform 2 n=1 Tax=Homo sapiens RepID=A5JHP3_HUMAN Length: 121aa >DCD 1 n=3 Tax=Euarchontoglires RepID=DCD_HUMAN 10 UniRef100_B4E205 UniRef100_O confidence: 1.00 max coverage: 8.4% num unique peps: 5 tot indep spectra: 7 share of spectrum id's: 3.34% >cdna FLJ61651, highly similar to Protein transport protein Sec24A n=1 Tax=Homo sapiens RepID=B4E205_HUMAN Length: 857aa >Protein transport protein Sec24A n=1 Tax=Homo sapiens RepID=SC24A_HUMAN 11 UniRef100_P confidence: 1.00 coverage: 6.0% num unique peps: 8 tot indep spectra: 14 share of spectrum id's: 7.14% subsumed entries: 5
7 >Myosin 9 n=2 Tax=Homo sapiens RepID=MYH9_HUMAN Length: 1960aa 12 UniRef100_UPI0001D confidence: 0.21 coverage: 5.8% num unique peps: 1 tot indep spectra: 2 share of spectrum id's: 1.03% >fab fragment of anti Shh 5E1 mouse/human chim n=1 Tax=Mus musculus RepID=UPI0001D34140 Length: 223aa 13 UniRef100_P confidence: 1.00 coverage: 5.7% num unique peps: 3 tot indep spectra: 5 share of spectrum id's: 2.59% subsumed entries: 1 >52 kda Ro protein n=1 Tax=Homo sapiens RepID=RO52_HUMAN 475aa Length: 14 UniRef100_P confidence: 1.00 coverage: 5.6% num unique peps: 1 tot indep spectra: 1 share of spectrum id's: 1.51% >Keratin, type II cytoskeletal 5 n=1 Tax=Homo sapiens RepID=K2C5_HUMAN Length: 590aa 15 UniRef100_D3Y confidence: 0.23 coverage: 4.9% num unique peps: 1 tot indep spectra: 2 share of spectrum id's: 1.04% >Trypsingon (Fragment) n=1 Tax=Branchiostoma belcheri RepID=D3Y298_BRABE Length: 204aa 16 UniRef100_A0JLS9 UniRef100_B2RBI8 UniRef100_B4DGU2 UniRef100_B4DJV0 UniRef100_B4DS26 UniRef100_B4DSB8 UniRef100_B4DUW9 UniRef100_P37275 UniRef100_Q13088 UniRef100_Q2KJ05 UniRef100_Q32Q63 UniRef100_Q5VZ84
8 UniRef100_Q8NB68 UniRef100_UPI C UniRef100_UPI0001CE91A2 UniRef100_UPI0001CE91A confidence: 0.10 max coverage: 2.0% num unique peps: 1 tot indep spectra: 1 share of spectrum id's: 0.52% >ZEB1 protein (Fragment) n=1 Tax=Homo sapiens RepID=A0JLS9_HUMAN Length: 1059aa >cdna, FLJ95535, highly similar to Homo sapiens transcription factor 8 (represses interleukin 2 expression) (TCF8), mrna n=1 Tax=Homo sapiens RepID=B2RBI8_HUMAN >cdna FLJ61244, highly similar to Transcription factor 8 n=1 Tax=Homo sapiens RepID=B4DGU2_HUMAN >cdna FLJ50550, highly similar to Transcription factor 8 (Fragment) n=1 Tax=Homo sapiens RepID=B4DJV0_HUMAN >cdna FLJ50636, highly similar to Transcription factor 8 n=1 Tax=Homo sapiens RepID=B4DS26_HUMAN >cdna FLJ50640, highly similar to Transcription factor 8 (Fragment) n=1 Tax=Homo sapiens RepID=B4DSB8_HUMAN >cdna FLJ50696, highly similar to Transcription factor 8 n=1 Tax=Homo sapiens RepID=B4DUW9_HUMAN >Zinc finger E box binding homeobox 1 n=1 Tax=Homo sapiens RepID=ZEB1_HUMAN >ZEB (Fragment) n=1 Tax=Homo sapiens RepID=Q13088_HUMAN >Zinc finger E box binding homeobox 1 n=1 Tax=Homo sapiens RepID=Q2KJ05_HUMAN >ZEB1 protein (Fragment) n=1 Tax=Homo sapiens RepID=Q32Q63_HUMAN >Zinc finger E box binding homeobox 1 n=2 Tax=Homo sapiens RepID=Q5VZ84_HUMAN >cdna FLJ34159 fis, clone FCBBF , highly similar to NIL 2 A ZINC FINGER PROTEIN n=1 Tax=Homo sapiens RepID=Q8NB68_HUMAN >zinc finger E box binding homeobox 1 isoform a n=2 Tax=Homo sapiens RepID=UPI C >zinc finger E box binding homeobox 1 isoform d n=1 Tax=Homo sapiens RepID=UPI0001CE91A2
9 >zinc finger E box binding homeobox 1 isoform e n=1 Tax=Homo sapiens RepID=UPI0001CE91A3 MS results for upper band (~200 kda): 1 UniRef100_P confidence: 1.00 coverage: 50.3% num unique peps: 41 tot indep spectra: 106 share of spectrum id's: 23.02% subsumed entries: 1 >Keratin, type II cytoskeletal 1 n=1 Tax=Homo sapiens RepID=K2C1_HUMAN Length: 644aa 2 UniRef100_P confidence: 1.00 coverage: 44.6% num unique peps: 25 tot indep spectra: 58 share of spectrum id's: 12.41% >Keratin, type I cytoskeletal 9 n=1 Tax=Homo sapiens RepID=K1C9_HUMAN Length: 623aa 3 UniRef100_P13645 UniRef100_UPI00017BCE7F confidence: 1.00 max coverage: 35.4% num unique peps: 23 tot indep spectra: 62 share of spectrum id's: 13.17% >Keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=K1C10_HUMAN Length: 584aa >keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=UPI00017BCE7F 4 UniRef100_P confidence: 1.00 coverage: 32.6% num unique peps: 22 tot indep spectra: 47 share of spectrum id's: 9.82% subsumed entries: 6 >Serum albumin n=1 Tax=Bos taurus RepID=ALBU_BOVIN Length: 607aa
10 5 UniRef100_C5IWV5 UniRef100_P confidence: 1.00 max coverage: 28.6% num unique peps: 18 tot indep spectra: 45 share of spectrum id's: 6.62% >Trypsinogen n=1 Tax=Sus scrofa RepID=C5IWV5_PIG Length: 246aa >Trypsin n=1 Tax=Sus scrofa RepID=TRYP_PIG 6 UniRef100_P confidence: 1.00 coverage: 25.8% num unique peps: 12 tot indep spectra: 29 share of spectrum id's: 6.42% >Keratin, type II cytoskeletal 2 epidermal n=1 Tax=Homo sapiens RepID=K22E_HUMAN Length: 639aa 7 UniRef100_P confidence: 1.00 coverage: 25.0% num unique peps: 4 tot indep spectra: 7 share of spectrum id's: 2.78% subsumed entries: 10 >Keratin, type I cytoskeletal 14 n=1 Tax=Homo sapiens RepID=K1C14_HUMAN Length: 472aa 8 UniRef100_A1E282 UniRef100_B4DVQ0 UniRef100_B4E335 UniRef100_B4E3A4 UniRef100_P60709 UniRef100_P63261 UniRef100_Q4R4H6 UniRef100_Q53G76 UniRef100_Q53G99 UniRef100_Q53GK6 UniRef100_Q8WVW5 UniRef100_UPI0000E0A7CD UniRef100_UPI000186E confidence: 1.00 max coverage: 24.0% num unique peps: 2 tot indep spectra: 2 share of spectrum id's: 0.40% >Beta actin (Fragment) n=4 Tax=Coelomata RepID=A1E282_HUMAN Length: 121aa >cdna FLJ58286, highly similar to Actin, cytoplasmic 2 n=1 Tax=Homo sapiens RepID=B4DVQ0_HUMAN
11 >cdna FLJ52842, highly similar to Actin, cytoplasmic 1 n=1 Tax=Homo sapiens RepID=B4E335_HUMAN >cdna FLJ57283, highly similar to Actin, cytoplasmic 2 n=1 Tax=Homo sapiens RepID=B4E3A4_HUMAN >Actin, cytoplasmic 1, N terminally processed n=24 Tax=Amniota RepID=ACTB_HUMAN >Actin, cytoplasmic 2, N terminally processed n=9 Tax=Tetrapoda RepID=ACTG_HUMAN >Brain cdna, clone: QflA 11253, similar to human actin, gamma 1 (ACTG1), n=1 Tax=Macaca fascicularis RepID=Q4R4H6_MACFA >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G76_HUMAN >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G99_HUMAN >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53GK6_HUMAN >Putative uncharacterized protein (Fragment) n=1 Tax=Homo sapiens RepID=Q8WVW5_HUMAN >UPI0000E0A7CD related cluster n=1 Tax=Homo sapiens RepID=UPI0000E0A7CD >Actin 5C n=1 Tax=Pediculus humanus corporis RepID=UPI000186E470 9 UniRef100_P confidence: 1.00 coverage: 20.9% num unique peps: 3 tot indep spectra: 5 share of spectrum id's: 2.33% subsumed entries: 2 >Keratin, type I cytoskeletal 16 n=1 Tax=Homo sapiens RepID=K1C16_HUMAN Length: 473aa 10 UniRef100_P confidence: 1.00 coverage: 17.3% num unique peps: 7 tot indep spectra: 13 share of spectrum id's: 3.72% subsumed entries: 2 >Keratin, type II cytoskeletal 5 n=1 Tax=Homo sapiens RepID=K2C5_HUMAN Length: 590aa 11 UniRef100_P
12 confidence: 1.00 coverage: 14.3% num unique peps: 24 tot indep spectra: 47 share of spectrum id's: 10.22% subsumed entries: 10 >Myosin 9 n=2 Tax=Homo sapiens RepID=MYH9_HUMAN Length: 1960aa 12 UniRef100_P confidence: 1.00 coverage: 9.7% num unique peps: 4 tot indep spectra: 10 share of spectrum id's: 2.23% subsumed entries: 1 >52 kda Ro protein n=1 Tax=Homo sapiens RepID=RO52_HUMAN 475aa Length: 13 UniRef100_A8K008 UniRef100_P01857 UniRef100_P01859 UniRef100_P01861 UniRef100_Q5EFE5 UniRef100_Q6GMX6 UniRef100_Q6MZU6 UniRef100_Q6MZV7 UniRef100_Q6MZX7 UniRef100_Q6N089 UniRef100_Q6N093 UniRef100_UPI A4 UniRef100_UPI000173A69D UniRef100_UPI000173A69E UniRef100_UPI000195C290 UniRef100_UPI0001A5EB confidence: 1.00 max coverage: 8.3% num unique peps: 3 tot indep spectra: 7 share of spectrum id's: 1.33% >cdna FLJ78387 n=1 Tax=Homo sapiens RepID=A8K008_HUMAN 472aa Length: >Ig gamma 1 chain C region n=1 Tax=Homo sapiens RepID=IGHG1_HUMAN >Ig gamma 2 chain C region n=1 Tax=Homo sapiens RepID=IGHG2_HUMAN >Ig gamma 4 chain C region n=1 Tax=Homo sapiens RepID=IGHG4_HUMAN >Anti RhD monoclonal T125 gamma1 heavy chain n=1 Tax=Homo sapiens RepID=Q5EFE5_HUMAN >IGH@ protein n=1 Tax=Homo sapiens RepID=Q6GMX6_HUMAN >Putative uncharacterized protein DKFZp686C15213 n=1 Tax=Homo sapiens RepID=Q6MZU6_HUMAN >Putative uncharacterized protein DKFZp686C11235 n=1 Tax=Homo sapiens RepID=Q6MZV7_HUMAN >Putative uncharacterized protein DKFZp686M24218 n=1 Tax=Homo sapiens RepID=Q6MZX7_HUMAN
13 >Putative uncharacterized protein DKFZp686P15220 n=1 Tax=Homo sapiens RepID=Q6N089_HUMAN >Putative uncharacterized protein DKFZp686I04196 (Fragment) n=1 Tax=Homo sapiens RepID=Q6N093_HUMAN >IGG1 MCG INTACT ANTIBODY (HEAVY CHAIN) n=1 Tax=Homo sapiens RepID=UPI A4 >Immunoglobulin heavy chain C gene segment n=1 Tax=Homo sapiens RepID=UPI000173A69D >Immunoglobulin heavy chain C gene segment n=1 Tax=Homo sapiens RepID=UPI000173A69E >IG GAMMA 1 CHAIN C REGION n=1 Tax=Homo sapiens RepID=UPI000195C290 >PREDICTED: similar to immunoglobulin lambda like polypeptide 1 n=1 Tax=Homo sapiens RepID=UPI0001A5EB10 14 UniRef100_A0JLS9 UniRef100_B2RBI8 UniRef100_B4DS26 UniRef100_B4DSB8 UniRef100_B4DUW9 UniRef100_P37275 UniRef100_Q13088 UniRef100_Q2KJ05 UniRef100_Q32Q63 UniRef100_Q5VZ84 UniRef100_Q8NB68 UniRef100_UPI C UniRef100_UPI0001CE91A2 UniRef100_UPI0001CE91A confidence: 0.08 max coverage: 5.1% num unique peps: 1 tot indep spectra: 1 share of spectrum id's: 0.22% >ZEB1 protein (Fragment) n=1 Tax=Homo sapiens RepID=A0JLS9_HUMAN Length: 1059aa >cdna, FLJ95535, highly similar to Homo sapiens transcription factor 8 (represses interleukin 2 expression) (TCF8), mrna n=1 Tax=Homo sapiens RepID=B2RBI8_HUMAN >cdna FLJ50636, highly similar to Transcription factor 8 n=1 Tax=Homo sapiens RepID=B4DS26_HUMAN >cdna FLJ50640, highly similar to Transcription factor 8 (Fragment) n=1 Tax=Homo sapiens RepID=B4DSB8_HUMAN >cdna FLJ50696, highly similar to Transcription factor 8 n=1 Tax=Homo sapiens RepID=B4DUW9_HUMAN
14 >Zinc finger E box binding homeobox 1 n=1 Tax=Homo sapiens RepID=ZEB1_HUMAN >ZEB (Fragment) n=1 Tax=Homo sapiens RepID=Q13088_HUMAN >Zinc finger E box binding homeobox 1 n=1 Tax=Homo sapiens RepID=Q2KJ05_HUMAN >ZEB1 protein (Fragment) n=1 Tax=Homo sapiens RepID=Q32Q63_HUMAN >Zinc finger E box binding homeobox 1 n=2 Tax=Homo sapiens RepID=Q5VZ84_HUMAN >cdna FLJ34159 fis, clone FCBBF , highly similar to NIL 2 A ZINC FINGER PROTEIN n=1 Tax=Homo sapiens RepID=Q8NB68_HUMAN >zinc finger E box binding homeobox 1 isoform a n=2 Tax=Homo sapiens RepID=UPI C >zinc finger E box binding homeobox 1 isoform d n=1 Tax=Homo sapiens RepID=UPI0001CE91A2 >zinc finger E box binding homeobox 1 isoform e n=1 Tax=Homo sapiens RepID=UPI0001CE91A3 15 UniRef100_Q5DT20 UniRef100_Q86YZ confidence: 0.89 max coverage: 1.7% num unique peps: 2 tot indep spectra: 2 share of spectrum id's: 0.44% >Hornerin n=1 Tax=Homo sapiens RepID=Q5DT20_HUMAN Length: 2850aa >Hornerin n=1 Tax=Homo sapiens RepID=HORN_HUMAN
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
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