ENCODE DCC Antibody Validation Document
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1 ENCODE DCC Antibody Validation Document Date of Submission Name: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description: Species Target Species Host Validation Method #1 Validation Method #2 Purification Method Polyclonal/ Monoclonal Vendor URL: Reference (PI/ Publication Information) Please complete the following for antibodies to histone modifications: if your specifications are not listed in the drop-down box, please write-in the appropriate information Histone Name AA modified AA Position Modification
2 Validation #1 Analysis Insert Validation Image (click here)
3 Validation #2 Analysis Insert Validation Image (Click here)
4 Identified Proteins (117) Accession Number Molecular Weight ZBTB33 Heat shock protein HSP 90-beta n=1 Tax=Homo sapiens RepID=HS90B_HUMAN P kda 27 Nucleolar RNA helicase 2 n=2 Tax=Homo sapiens RepID=DDX21_HUMAN Q9NR30 87 kda 24 Elongation factor 2 n=2 Tax=Hominidae RepID=EF2_HUMAN P kda 21 cdna FLJ76789, highly similar to Homo sapiens methionine-trna synthetase (MARS), mrna n=1 Tax=Homo sapiens RepID=A8K492_HUMAN Dopamine receptor interacting protein 4 n=1 Tax=Homo sapiens RepID=Q4W4Y1_HUMAN A8K492 (+1) 101 kda 15 Q4W4Y1 (+1) 96 kda 10 Heat shock protein HSP 90-alpha n=2 Tax=Homo sapiens RepID=HS90A_HUMAN P kda 10 Transportin-1 n=2 Tax=Homo sapiens RepID=TNPO1_HUMAN Q kda 10 Endoplasmin n=1 Tax=Homo sapiens RepID=ENPL_HUMAN P14625 (+1) 92 kda 9 DNA replication licensing factor MCM6 n=2 Tax=Homo sapiens RepID=MCM6_HUMAN Q kda 9 26S proteasome non-atpase regulatory subunit 2 n=2 Tax=Homininae RepID=PSMD2_HUMAN cdna, FLJ93378, highly similar to Homo sapiens MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) (MCM3), mrna n=1 Tax=Homo sapiens RepID=B2R7C5_HUMAN cdna FLJ75881, highly similar to Homo sapiens transferrin receptor (p90, CD71) (TFRC), mrna n=1 Tax=Homo sapiens RepID=A8K6Q8_HUMAN cdna FLJ78580, highly similar to Homo sapiens MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (MCM5), mrna n=1 Tax=Homo sapiens RepID=A8K521_HUMAN cdna FLJ54365, highly similar to DNA replication licensing factor MCM4 n=1 Tax=Homo sapiens RepID=B4DLA6_HUMAN Q13200 (+2) 100 kda 7 B2R7C5 (+3) 91 kda 7 A8K6Q8 (+1) 85 kda 6 A8K521 (+5) 82 kda 6 B4DLA6 (+3) 93 kda 6 Serum albumin n=1 Tax=Bos taurus RepID=ALBU_BOVIN P kda 5
5 Keratin, type II cytoskeletal 5 n=1 Tax=Homo sapiens RepID=K2C5_HUMAN P kda 5 Keratin, type I cytoskeletal 14 n=1 Tax=Homo sapiens RepID=K1C14_HUMAN P kda 5 cdna, FLJ92955, highly similar to Homo sapiens transportin-sr (TRN-SR), mrna n=1 Tax=Homo sapiens RepID=B2R6H7_HUMAN cdna FLJ61290, highly similar to Neutral alphaglucosidase AB n=1 Tax=Homo sapiens RepID=B4DJ30_HUMAN Kaiso cdna FLJ12872 fis, clone NT2RP , highly similar to COATOMER GAMMA SUBUNIT n=1 Tax=Homo sapiens RepID=Q9H9B7_HUMAN Splicing factor, proline- and glutamine-rich n=1 Tax=Homo sapiens RepID=SFPQ_HUMAN cdna, FLJ95650, highly similar to Homo sapiens karyopherin (importin) beta 1 (KPNB1), mrna n=1 Tax=Homo sapiens RepID=B2RBR9_HUMAN B2R6H7 (+2) 104 kda 5 B4DJ30 (+2) 113 kda 5 GB0002 (+1) 75 kda 5 Q9H9B7 (+1) 89 kda 5 P23246 (+2) 76 kda 4 B2RBR9 (+1) 97 kda 4 DNA topoisomerase 1 n=1 Tax=Homo sapiens RepID=TOP1_HUMAN P kda 4 cdna FLJ78686, highly similar to Homo sapiens nucleoporin 93kDa (NUP93), mrna n=1 Tax=Homo sapiens RepID=A8K897_HUMAN cdna FLJ75831, highly similar to Homo sapiens exportin, trna (nuclear export receptor for trnas) (XPOT), mrna n=1 Tax=Homo sapiens RepID=A8KA19_HUMAN X-ray repair cross-complementing protein 5 n=1 Tax=Homo sapiens RepID=XRCC5_HUMAN cdna FLJ75085, highly similar to Homo sapiens glutaminyl-trna synthetase (QARS), mrna n=1 Tax=Homo sapiens RepID=A8K3A8_HUMAN COPG protein (Fragment) n=2 Tax=Homininae RepID=Q8WUI6_HUMAN cdna, FLJ95468, highly similar to Homo sapiens transcriptional coactivator tubedown-100 (TBDN100),transcript variant 1, mrna n=1 Tax=Homo sapiens RepID=B2RBE5_HUMAN A8K897 (+2) 93 kda 4 A8KA19 (+1) 110 kda 4 P13010 (+1) 83 kda 4 A8K3A8 (+4) 88 kda 4 Q8WUI6 (+1) 78 kda 4 B2RBE5 (+2) 101 kda 4
6 Clathrin heavy chain 1 n=3 Tax=Eutheria RepID=CLH1_HUMAN Q00610 (+2) 192 kda 3 Coatomer subunit beta n=2 Tax=Homo sapiens RepID=COPB_HUMAN P kda 3 cdna FLJ55635, highly similar to pre-mrnasplicing factoratp-dependent RNA helicase DHX15 (EC ) n=1 Tax=Homo sapiens RepID=B4E0S6_HUMAN Putative uncharacterized protein HEATR2 n=1 Tax=Homo sapiens RepID=C9J0D8_HUMAN DEAD box polypeptide 27 n=1 Tax=Homo sapiens RepID=B3GQE6_HUMAN Putative uncharacterized protein ZC3HAV1 n=1 Tax=Homo sapiens RepID=C9J6P4_HUMAN cdna FLJ76184, highly similar to Homo sapiens NOL1/NOP2/Sun domain family, member 2 (NSUN2), mrna n=1 Tax=Homo sapiens RepID=A8K529_HUMAN cdna, FLJ95407, highly similar to Homo sapiens MCM7 minichromosome maintenance deficient 7 (S. cerevisiae) (MCM7), mrna n=1 Tax=Homo sapiens RepID=B2RBA6_HUMAN B4E0S6 (+1) 90 kda 3 C9J0D8 (+5) 92 kda 3 B3GQE6 (+3) 87 kda 3 C9J6P4 (+2) 114 kda 3 A8K529 (+3) 86 kda 3 B2RBA6 (+1) 81 kda 3
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
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SUPPLEMENTARY INFORMATION
doi:10.1038/nature12890 Supplementary Table 1 Summary of protein components in the 39S subunit model. MW, molecular weight; aa amino acids; RP, ribosomal protein. Protein* MRP size MW (kda) Sequence accession