ENCODE DCC Antibody Validation Document
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1 ENCODE DCC Antibody Validation Document Date of Submission Name: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description: Species Target Species Host Validation Method #1 Validation Method #2 Purification Method Polyclonal/ Monoclonal Vendor URL: Reference (PI/ Publication Information) Please complete the following for antibodies to histone modifications: if your specifications are not listed in the drop-down box, please write-in the appropriate information Histone Name AA modified AA Position Modification
2 Validation #1 Analysis Insert Validation Image (click here)
3 Validation #2 Analysis Insert Validation Image (Click here)
4 19 entries (9 single hits) retrieved from /home/tpp/tpp/ _bowling/int-gb_ _ltq_bowling_mta3-sequest.prot.xml * corresponds to peptide is_nondegenerate_evidence flag 1 UniRef100_B4DHT9 UniRef100_B4DU71 UniRef100_P14625 UniRef100_Q59FC6 UniRef100_Q5CAQ5 UniRef100_UPI0001AE6A99 1 confidence: 1. max coverage:num unique petot indep spec share of spectrum id's: 2.00% >cdna FLJ58812, highly similar to Endoplasmin (Heat shock protein 90kDa beta member 1) n=1 Tax=Homo sapiens Length: 468aa >cdna FLJ58626, highly similar to Endoplasmin (Heat shock protein 90 kda beta member 1) n=1 Tax=Homo sapiens RepID=B4DU71_HUMAN >Endoplasmin n=1 Tax=Homo sapiens RepID=ENPL_HUMAN >Tumor rejection antigen (Gp96) 1 variant (Fragment) n=1 Tax=Homo sapiens RepID=Q59FC6_HUMAN >Tumor rejection antigen (Gp96) 1 n=1 Tax=Homo sapiens RepID=Q5CAQ5_HUMAN >UPI0001AE6A99 related cluster n=1 Tax=Homo sapiens RepID=UPI0001AE6A99 2 UniRef100_P confidence: 1. coverage: 11.6num unique petot indep spec share of spectrum id's: 3.72% >Keratin, type I cytoskeletal 9 n=1 Tax=Homo sapiens RepID=K1C9_HUMAN Length: 623aa 4a UniRef100_B3GQS7 UniRef100_B7Z597 UniRef100_B7Z5E7 UniRef100_P confidence: 1. max coverage:num unique petot indep spec share of spectrum id's: 3.68% >Mitochondrial heat shock 60kD protein 1 variant 1 n=1 Tax=Homo sapiens RepID=B3GQS7_HUMAN Length: 569aa >cdna FLJ54373, highly similar to 60 kda heat shock protein, mitochondrial n=1 Tax=Homo sapiens RepID=B7Z597_HUMAN >cdna FLJ51046, highly similar to 60 kda heat shock protein, mitochondrial n=1 Tax=Homo sapiens RepID=B7Z5E7_HUMAN >60 kda heat shock protein, mitochondrial n=1 Tax=Homo sapiens RepID=CH60_HUMAN 5a UniRef100_B4DNL5 UniRef100_P confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 6 >cdna FLJ59361, highly similar to Protein disulfide-isomerase (EC ) n=1 Tax=Homo sapiens RepID=B4DNL5_Length: 492aa >Protein disulfide-isomerase n=1 Tax=Homo sapiens RepID=PDIA1_HUMAN 6a UniRef100_C5IWV5 UniRef100_P confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 1 >Trypsinogen n=1 Tax=Sus scrofa RepID=C5IWV5_PIG >Trypsin n=1 Tax=Sus scrofa RepID=TRYP_PIG Length: 246aa 7a UniRef100_P confidence: 1. coverage: 31.5num unique petot indep spec share of spect subsumed entries: 1 >Serum albumin n=1 Tax=Bos taurus RepID=ALBU_BOVIN Length: 607aa 8a UniRef100_P confidence: 1. coverage: 25.0num unique petot indep spec share of spectrum id's: 9.99% >Keratin, type II cytoskeletal 1 n=1 Tax=Homo sapiens RepID=K2C1_HUMAN Length: 644aa 8b UniRef100_P confidence: 1. coverage: 16.0num unique petot indep spec share of spectrum id's: 2.37% >Keratin, type II cytoskeletal 2 epidermal n=1 Tax=Homo sapiens RepID=K22E_HUMAN Length: 639aa 8c UniRef100_P confidence: 1. coverage: 12.2num unique petot indep spec share of spect subsumed entries: 2 >Keratin, type II cytoskeletal 5 n=1 Tax=Homo sapiens RepID=K2C5_HUMAN Length: 590aa 9a UniRef100_P13645 UniRef100_UPI00017BCE7F confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 1 >Keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=K1C10_HUMAN >keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=UPI00017BCE7F Length: 584aa 10a UniRef100_P confidence: 1. coverage: 10.0num unique petot indep spec share of spect subsumed entries: 9 >Pyruvate kinase isozymes M1/M2 n=2 Tax=Homininae RepID=KPYM_HUMAN Length: 531aa 12a UniRef100_Q9BTC8 UniRef100_Q9BTC8-2 UniRef100_UPI0000D4CDC7 UniRef100_UPI confidence: 1max coverag num unique ptot indep speshare of specsubsumed entries: 6 >Metastasis-associated protein MTA3 n=3 Tax=Homininae RepID=MTA3_HUMAN Length: 594aa >Isoform 2 of Metastasis-associated protein MTA3 n=1 Tax=Homo sapiens RepID=Q9BTC8-2 >UPI0000D4CDC7 related cluster n=1 Tax=Homo sapiens RepID=UPI0000D4CDC7 >UPI related cluster n=1 Tax=Homo sapiens RepID=UPI
5 13a UniRef100_UPI UniRef100_UPI00017BDB3D UniRef100_UPI00017BDB confidence: 1. max coverage:num unique petot indep spec share of spectrum id's: 0.56% >MONOCLONAL ANTIBODY MAK33 n=1 Tax=Homo sapiens RepID=UPI >FabOX108 Light Chain Fragment n=1 Tax=Homo sapiens RepID=UPI00017BDB3D >FabOX117 Light Chain Fragment n=1 Tax=Homo sapiens RepID=UPI00017BDB42 Length: 213aa 11a UniRef100_P confidence: 1. coverage: 5.3%num unique petot indep spec share of spect subsumed entries: 2 >Protein disulfide-isomerase A3 n=2 Tax=Hominidae RepID=PDIA3_HUMAN Length: 505aa 6b UniRef100_P confidence: 1. coverage: 6.9%num unique petot indep spec share of spectrum id's: 1.82% >Anionic trypsin n=2 Tax=Canis lupus familiaris RepID=TRY2_CANFA Length: 247aa 8d UniRef100_A8K2I0 UniRef100_B2R853 UniRef100_B4DRR0 UniRef100_B4DRU6 UniRef100_B4DRY0 UniRef100_B4DWU6 UniRef100_P02538 UniRef100_P48668 UniRef100_UPI0001AE6B0A UniRef100_UPI0001AE6B0B confidence: 1. max coverage:num unique petot indep spec share of spectrum id's: 1.23% >cdna FLJ78504, highly similar to Homo sapiens keratin 6A (KRT6A), mrna n=1 Tax=Homo sapiens RepID=A8K2I0 Length: 564aa >cdna, FLJ93744, highly similar to Homo sapiens keratin 6E (KRT6E), mrna n=1 Tax=Homo sapiens RepID=B2R853_HUMAN >cdna FLJ53910, highly similar to Keratin, type II cytoskeletal 6A n=1 Tax=Homo sapiens RepID=B4DRR0_HUMAN >cdna FLJ54657, highly similar to Keratin, type II cytoskeletal 6A n=1 Tax=Homo sapiens RepID=B4DRU6_HUMAN >cdna FLJ54379, highly similar to Keratin, type II cytoskeletal 6E n=1 Tax=Homo sapiens RepID=B4DRY0_HUMAN >cdna FLJ51361, highly similar to Keratin, type II cytoskeletal 6A n=1 Tax=Homo sapiens RepID=B4DWU6_HUMAN >Keratin, type II cytoskeletal 6A n=1 Tax=Homo sapiens RepID=K2C6A_HUMAN >Keratin, type II cytoskeletal 6C n=1 Tax=Homo sapiens RepID=K2C6C_HUMAN >UPI0001AE6B0A related cluster n=1 Tax=Homo sapiens RepID=UPI0001AE6B0A >UPI0001AE6B0B related cluster n=1 Tax=Homo sapiens RepID=UPI0001AE6B0B 9b UniRef100_P confidence: 1. coverage: 7.0%num unique petot indep spec share of spect subsumed entries: 2 >Keratin, type I cytoskeletal 14 n=1 Tax=Homo sapiens RepID=K1C14_HUMAN Length: 472aa 9c UniRef100_P confidence: 1. coverage: 6.8%num unique petot indep spec share of spectrum id's: 0.84% >Keratin, type I cytoskeletal 16 n=1 Tax=Homo sapiens RepID=K1C16_HUMAN Length: 473aa 14a UniRef100_UPI000186EE confidence: 0. coverage: 4.9%num unique petot indep spec share of spect subsumed entries: 1 >UDP-glucose 6-dehydrogenase, putative n=1 Tax=Pediculus humanus corporis RepID=UPI000186EE46 Length: 473aa 15 UniRef100_B4DI25 UniRef100_B7Z2E9 UniRef100_B7Z9Y8 UniRef100_Q05BL1 UniRef100_Q13625 UniRef100_Q UniRef100_UPI0000D620BA UniRef100_UPI00015E0CE confidence: 0. max coverage:num unique petot indep spec share of spectrum id's: 0.56% >cdna FLJ51856, highly similar to Apoptosis-stimulating of p53 protein 2 n=1 Tax=Homo sapiens RepID=B4DI25_H Length: 723aa >cdna FLJ50500, highly similar to Apoptosis-stimulating of p53 protein 2 n=1 Tax=Homo sapiens RepID=B7Z2E9_HUMAN >cdna, FLJ79002, highly similar to Apoptosis-stimulating of p53 protein 2 n=1 Tax=Homo sapiens RepID=B7Z9Y8_HUMAN >TP53BP2 protein n=1 Tax=Homo sapiens RepID=Q05BL1_HUMAN >Apoptosis-stimulating of p53 protein 2 n=2 Tax=Homo sapiens RepID=ASPP2_HUMAN >Isoform 2 of Apoptosis-stimulating of p53 protein 2 n=2 Tax=Homo sapiens RepID=Q >Apoptosis-stimulating of p53 protein 2 (Tumor suppressor p53-binding protein 2) (p53-binding protein 2) (p53bp2) (53BP2) (Bcl2-binding protein) (Bbp) (Renal carcinoma antigen NY-REN-51). n=1 Tax=Homo sapiens RepID=UPI0000D620BA >Apoptosis-stimulating of p53 protein 2 (Tumor suppressor p53-binding protein 2) (p53-binding protein 2) (p53bp2) (53BP2) (Bcl2-binding protein) (Bbp) (Renal carcinoma antigen NY-REN-51). n=1 Tax=Homo sapiens RepID=UPI00015E0CE3 16 UniRef100_UPI0000E1F confidence: 0. coverage: 2.7%num unique petot indep spec share of spectrum id's: 0.85% >PREDICTED: similar to [Human Ig rearranged gamma chain mrna, V-J-C region and complete cds.], gene product Length: 415aa 17 UniRef100_UPI000186CD8C confidence: 0. coverage: 1.3%num unique petot indep spec share of spectrum id's: 0.29% >protein disulfide isomerase, putative n=1 Tax=Pediculus humanus corporis RepID=UPI000186CD8C Length: 630aa 18 UniRef100_A5JHP3 UniRef100_P confidence: 0. max coverage:num unique petot indep spec share of spectrum id's: 0.29% >Dermcidin isoform 2 n=1 Tax=Homo sapiens RepID=A5JHP3_HUMAN >DCD-1 n=3 Tax=Euarchontoglires RepID=DCD_HUMAN Length: 121aa 19 UniRef100_A8K854 UniRef100_B2RBD5 UniRef100_B3KML9 UniRef100_B4DE77 UniRef100_B4DQN9 UniRef100_B4DY90 UniRef100_B4E052 UniRef100_B7ZAF0 UniRef100_B7ZAK1 UniRef100_P04350 UniRef100_P07437 UniRef100_P68371 UniRef100_Q13509 UniRef100_Q1KSF8 UniRef100_Q3ZCR3 UniRef100_Q53G92 UniRef100_Q5JP53 UniRef100_Q5ST81
6 UniRef100_Q6LC01 UniRef100_Q7KZS6 UniRef100_Q8IWP6 UniRef100_Q8IZ29 UniRef100_Q8N6N5 UniRef100_UPI000186CB60 UniRef100_UPI000186D confidence: 0. max coverage:num unique petot indep spec share of spectrum id's: 0.57% >HCG , isoform CRA_f n=1 Tax=Homo sapiens RepID=A8K854_HUMAN Length: 378aa >cdna, FLJ95457, highly similar to Homo sapiens tubulin, beta, 4 (TUBB4), mrna n=1 Tax=Homo sapiens RepID=B2RBD5_HUMAN >cdna FLJ11352 fis, clone HEMBA , highly similar to Tubulin beta-2c chain n=1 Tax=Homo sapiens RepID=B3KML9_HUMAN >cdna FLJ55189, highly similar to Tubulin beta-4 chain n=1 Tax=Homo sapiens RepID=B4DE77_HUMAN >cdna FLJ50617, highly similar to Tubulin beta-7 chain n=1 Tax=Homo sapiens RepID=B4DQN9_HUMAN >cdna FLJ56903, highly similar to Tubulin beta-7 chain n=1 Tax=Homo sapiens RepID=B4DY90_HUMAN >cdna FLJ52378, highly similar to Tubulin beta-7 chain n=1 Tax=Homo sapiens RepID=B4E052_HUMAN >cdna, FLJ79164, highly similar to Tubulin beta-7 chain n=1 Tax=Homo sapiens RepID=B7ZAF0_HUMAN >cdna, FLJ79215, highly similar to Tubulin beta-7 chain n=1 Tax=Homo sapiens RepID=B7ZAK1_HUMAN >Tubulin beta-4 chain n=5 Tax=Eutheria RepID=TBB4_HUMAN >Tubulin beta chain n=12 Tax=Amniota RepID=TBB5_HUMAN >Tubulin beta-2c chain n=3 Tax=Eutheria RepID=TBB2C_HUMAN >Tubulin beta-3 chain n=4 Tax=Eutheria RepID=TBB3_HUMAN >XTP3TPA-transactivated protein 1 n=1 Tax=Homo sapiens RepID=Q1KSF8_HUMAN >TUBB3 protein (Fragment) n=1 Tax=Homo sapiens RepID=Q3ZCR3_HUMAN >Tubulin, beta, 4 variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G92_HUMAN >Tubulin beta polypeptide n=1 Tax=Homo sapiens RepID=Q5JP53_HUMAN >Tubulin beta polypeptide n=3 Tax=Hominidae RepID=Q5ST81_HUMAN >MRNA encoding beta-tubulin. (from clone D-beta-1) (Fragment) n=1 Tax=Homo sapiens RepID=Q6LC01_HUMAN >HCG n=1 Tax=Homo sapiens RepID=Q7KZS6_HUMAN >Class IVb beta tubulin n=1 Tax=Homo sapiens RepID=Q8IWP6_HUMAN >Tubulin, beta 2C n=1 Tax=Homo sapiens RepID=Q8IZ29_HUMAN >Tubulin, beta 2C n=1 Tax=Homo sapiens RepID=Q8N6N5_HUMAN >tubulin beta chain, putative n=1 Tax=Pediculus humanus corporis RepID=UPI000186CB60 >tubulin beta chain, putative n=1 Tax=Pediculus humanus corporis RepID=UPI000186D036 6c UniRef100_P06871 UniRef100_UPI00004A5B07 UniRef100_UPI00005A2FD6 UniRef100_UPI00005A2FD confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 1 >Cationic trypsin n=1 Tax=Canis lupus familiaris RepID=TRY1_CANFA Length: 246aa >PREDICTED: similar to trypsinogen 7 isoform 3 n=1 Tax=Canis lupus familiaris RepID=UPI00004A5B07 >PREDICTED: similar to trypsinogen 7 isoform 5 n=1 Tax=Canis lupus familiaris RepID=UPI00005A2FD6 >PREDICTED: similar to Cationic trypsin III precursor (Pretrypsinogen III) isoform 6 n=1 Tax=Canis lupus familiaris RepID=UPI00005A2FD7
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
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