Protein tyrosine phosphorylation in sperm during capacitation

Transcription

1 Chinese Bulletin of Life Sciences Vol. 18, No. 3 Jun., (2006) sac/camp/pka Q25 A Protein tyrosine phosphorylation in sperm during capacitation ZHOU Si-Chang, NI Ya, SHI Qi-Xian* (Zhejiang Academy of Medical Sciences, Hangzhou, China ) Abstract: Mammalian sperm are required to undergo a process known as capacitation before they can undertake the fertilization process. The protein phosphorylation especially at tyrosine residues is one of the most important events that occur during capacitation. The increase in protein tyrosine phosphorylation during capacitation has been shown to be regulated by camp/pka-dependent pathway, receptor tyrosine kinase pathway, and non-receptor protein tyrosine kinase pathway. It has been observed that the tyrosine phosphorylated proteins are localized in various regions of spermatozoon, and that the tyrosine phosphorylation of sperm proteins link to the different sperm functions. Key words: sperm sperm capacitation protein tyrosine phosphorylation; signal transduction (capacitation) (hyperactive motility) (acrosome reaction) ph Ca 2+ camp [1] / / (G ) ( ) (1980 ) (1961 ) (1936 ) *

2 286 Tash Means [2] / (Ser) (Thr) (Tyr) Ser/Thr Visconti [3] 40~120kDa Leyton Saling [4] (2-D) (Western blot) (MS/ MS) [5] 1 (sac)/ (camp)/ A(PKA) AC/cAMP/PKA 1.1 sac/camp/pka ( HCO 3 ) (soluble adenylate cyclase, sac) camp PKA A (AKAPs) ( ) camp H89(PKA ) / camp [6] PKA Ser/Thr [1] camp-pka 1.2 (receptor tyrosine kinases, RTKs) ( EGF IGF-1 p190 c-met c-abl ) (adapter protein)( Shc) Ras Raf MEK(MAPKK /extracellular signal-regulated kinase kinase) (mitogen-activated protein kinases, MAPK) ( ) MEK 1(extracellular signal-regulated protein kinase1 ERK1) ERK2 MAPK MAPK MAPK ERK2 Shc Ras [7] 1.3 ( c-yes TK-32) ( ) 2 [8] Urner [9] - Carrera [10] Naaby-Hansen [11] (CABYR) CABYR Ca 2+ [11] CABYR (pi4.0) 86kDa CABYR CABYR [11] Naz [12]

3 287 (endoplasmin, Erp99) 60(Hsp60) [13] [14] - 3 Leyton Saling [4] 52kDa 75kDa 95kDa 95kDa Naz [12] (95/94 3kDa 46 3kDa 25 7kDa 12 2kDa) 46 3 kda -1(FA-1) FA-1 - FA kda 46 3 kda(fa-1 ) [15] Flesch [16] (27kDa 37kDa 40kDa) (34kDa 47kDa 55kDa) Flesch [17] 34kDa 47kDa Hsp90 Ecroyd [18] Hsp90 Hsp86 Hsp86 ansamycin antibiotic geldanamycin Asquith [19] HspD1 TRA1(tumor rejection antigen, gp96) HspD1 Hsp60 TRA1 Erp99 Hsp60 Erp99 Erp99 Hsp90 Erp99 N (Grp94) Grp94 gp96 Erp 99 TRA1 Hsp90 Toll IgGs Asquith [13] Erp99 Hsp60, AKAPs AKAPs PKA Carrera [10] AKAP82 pro-akap82 AKAP3 AKAP4 [5] AKAPs ( ) - Luconi [20] HCO 3 sac AKAP3 PKA AKAP3 81kDa 95kDa 105kDa AKAP (FSP95) AKAP82 32% C II Mandal [21] FSP95 NagDas [22] (PHGPx) PHGPx PHGPx

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