ENCODE DCC Antibody Validation Document
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1 ENCODE DCC Antibody Validation Document Date of Submission Name: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description: Species Target Species Host Validation Method #1 Validation Method #2 Purification Method Polyclonal/ Monoclonal Vendor URL: Reference (PI/ Publication Information) Please complete the following for antibodies to histone modifications: if your specifications are not listed in the drop-down box, please write-in the appropriate information Histone Name AA modified AA Position Modification
2 Validation #1 Analysis Insert Validation Image (click here)
3 Validation #2 Analysis Insert Validation Image (Click here)
4 PML lower band MS results: 18 entries (7 single hits) retrieved from /home/tpp/tpp/ _bowling/int-gb_ _ltq_bowling_pml-1-sequest.prot.xml * corresponds to peptide is_nondegenerate_evidence flag 1 UniRef100_P confidence: 1. coverage: 18.0num unique petot indep spec share of spectrum id's: 7.31% >Keratin, type I cytoskeletal 9 n=1 Tax=Homo sapiens RepID=K1C9_HUMAN Length: 623aa 2 UniRef100_UPI000186E38A confidence: 1. coverage: 14.8num unique petot indep spec share of spectrum id's: 3.59% >Tropomyosin-2, putative n=1 Tax=Pediculus humanus corporis RepID=UPI000186E38A Length: 284aa 5a UniRef100_C5IWV5 UniRef100_P confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 2 >Trypsinogen n=1 Tax=Sus scrofa RepID=C5IWV5_PIG >Trypsin n=1 Tax=Sus scrofa RepID=TRYP_PIG 5b UniRef100_P confidence: 1. coverage: 6.9%num unique petot indep spec share of spectrum id's: 3.07% >Anionic trypsin n=2 Tax=Canis lupus familiaris RepID=TRY2_CANFA Length: 247aa 6a UniRef100_P confidence: 1. coverage: 21.4num unique petot indep spec share of spect subsumed entries: 6 >Serum albumin n=1 Tax=Bos taurus RepID=ALBU_BOVIN Length: 607aa 7a UniRef100_P confidence: 1. coverage: 23.3num unique petot indep spec share of spect subsumed entries: 1 >Keratin, type II cytoskeletal 1 n=1 Tax=Homo sapiens RepID=K2C1_HUMAN Length: 644aa 7b UniRef100_P confidence: 1. coverage: 12.2num unique petot indep spec share of spectrum id's: 2.12% >Keratin, type II cytoskeletal 2 epidermal n=1 Tax=Homo sapiens RepID=K22E_HUMAN Length: 639aa 8a UniRef100_P confidence: 1. coverage: 6.9%num unique petot indep spec share of spect subsumed entries: 2 >78 kda glucose-regulated protein n=1 Tax=Homo sapiens RepID=GRP78_HUMAN Length: 654aa 9a UniRef100_P13645 UniRef100_UPI00017BCE7F confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 2 >Keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=K1C10_HUMAN >keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=UPI00017BCE7F Length: 584aa 10a UniRef100_P29590 UniRef100_P UniRef100_P UniRef100_P UniRef100_P UniRef100_P UniRef100_Q15959 UniRef100_Q9BZX7 UniRef100_Q9BZY confidence: 1max coverag num unique ptot indep speshare of specsubsumed entries: 2 >Probable transcription factor PML n=1 Tax=Homo sapiens RepID=PML_HUMAN Length: 882aa >Isoform PML-2 of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >Isoform PML-3 of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >Isoform PML-3B of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >Isoform PML-X of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >Isoform PML-5 of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >PML protein n=1 Tax=Homo sapiens RepID=Q15959_HUMAN >Tripartite motif protein TRIM19 kappa n=1 Tax=Homo sapiens RepID=Q9BZX7_HUMAN >Tripartite motif protein TRIM19 beta n=1 Tax=Homo sapiens RepID=Q9BZY3_HUMAN 11a UniRef100_P35579 UniRef100_P confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 1 >Myosin-9 n=2 Tax=Homo sapiens RepID=MYH9_HUMAN >Isoform 2 of Myosin-9 n=1 Tax=Homo sapiens RepID=P Length: 1960aa 4a UniRef100_B3KTV0 UniRef100_B4DTX2 UniRef100_P11142 UniRef100_Q53GZ6 UniRef100_Q53HF2 UniRef100_UPI00015E00A confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 1 >cdna FLJ38781 fis, clone LIVER , highly similar to HEAT SHOCK COGNATE 71 kda PROTEIN n=1 Tax=HomoLength: 621aa >cdna FLJ59163, highly similar to Heat shock cognate 71 kda protein n=2 Tax=Euarchontoglires RepID=B4DTX2_HUMAN
5 >Heat shock cognate 71 kda protein n=8 Tax=Eutheria RepID=HSP7C_HUMAN >Heat shock 70kDa protein 8 isoform 1 variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53GZ6_HUMAN >Heat shock 70kDa protein 8 isoform 2 variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53HF2_HUMAN >Heat shock cognate 71 kda protein (Heat shock 70 kda protein 8). n=1 Tax=Homo sapiens RepID=UPI00015E00A1 12a UniRef100_UPI UniRef100_UPI00017BDB3D UniRef100_UPI00017BDB confidence: 1. max coverage:num unique petot indep spec share of spectrum id's: 1.36% >MONOCLONAL ANTIBODY MAK33 n=1 Tax=Homo sapiens RepID=UPI >FabOX108 Light Chain Fragment n=1 Tax=Homo sapiens RepID=UPI00017BDB3D >FabOX117 Light Chain Fragment n=1 Tax=Homo sapiens RepID=UPI00017BDB42 Length: 213aa 4b UniRef100_B2R6X5 UniRef100_B3KSM6 UniRef100_P17066 UniRef100_P48741 UniRef100_Q53FC confidence: 1. max coverage:num unique petot indep spec share of spectrum id's: 0.72% >cdna, FLJ93166, highly similar to Homo sapiens heat shock 70kDa protein 6 (HSP70B') (HSPA6), mrna n=1 Tax=HLength: 643aa >cdna FLJ36606 fis, clone TRACH , highly similar to HEAT SHOCK 70 kda PROTEIN 6 n=1 Tax=Homo sapiens RepID=B3KSM6_HUMAN >Heat shock 70 kda protein 6 n=1 Tax=Homo sapiens RepID=HSP76_HUMAN >Putative heat shock 70 kda protein 7 n=1 Tax=Homo sapiens RepID=HSP77_HUMAN >Heat shock 70kDa protein 6 (HSP70B') variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53FC7_HUMAN 13 UniRef100_C6L confidence: 0. coverage: 5.3%num unique petot indep spec share of spectrum id's: 1.04% >Putative trypsinogen n=1 Tax=Sus scrofa RepID=C6L245_PIG Length: 247aa 14 UniRef100_A5JHP3 UniRef100_P confidence: 0. max coverage:num unique petot indep spec share of spectrum id's: 1.08% >Dermcidin isoform 2 n=1 Tax=Homo sapiens RepID=A5JHP3_HUMAN >DCD-1 n=3 Tax=Euarchontoglires RepID=DCD_HUMAN Length: 121aa 15 UniRef100_UPI000186D confidence: 0. coverage: 3.2%num unique petot indep spec share of spectrum id's: 0.36% >conserved hypothetical protein n=1 Tax=Pediculus humanus corporis RepID=UPI000186D393 Length: 252aa 16 UniRef100_B4DVQ0 UniRef100_B4DW52 UniRef100_B4E335 UniRef100_B4E3A4 UniRef100_P60709 UniRef100_P63261 UniRef100_Q4R4H6 UniRef100_Q53G76 UniRef100_Q53G99 UniRef100_Q53GK6 UniRef100_Q6PJ43 UniRef100_Q8WVW5 UniRef100_UPI000186E confidence: 0. max coverage:num unique petot indep spec share of spectrum id's: 0.72% >cdna FLJ58286, highly similar to Actin, cytoplasmic 2 n=1 Tax=Homo sapiens RepID=B4DVQ0_HUMAN Length: 333aa >cdna FLJ55253, highly similar to Actin, cytoplasmic 1 n=1 Tax=Homo sapiens RepID=B4DW52_HUMAN >cdna FLJ52842, highly similar to Actin, cytoplasmic 1 n=1 Tax=Homo sapiens RepID=B4E335_HUMAN >cdna FLJ57283, highly similar to Actin, cytoplasmic 2 n=1 Tax=Homo sapiens RepID=B4E3A4_HUMAN >Actin, cytoplasmic 1, N-terminally processed n=24 Tax=Amniota RepID=ACTB_HUMAN >Actin, cytoplasmic 2, N-terminally processed n=9 Tax=Tetrapoda RepID=ACTG_HUMAN >Brain cdna, clone: QflA-11253, similar to human actin, gamma 1 (ACTG1), n=1 Tax=Macaca fascicularis RepID=Q4R4H6_MACFA >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G76_HUMAN >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G99_HUMAN >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53GK6_HUMAN >ACTG1 protein (Fragment) n=5 Tax=Eutheria RepID=Q6PJ43_HUMAN >Putative uncharacterized protein (Fragment) n=1 Tax=Homo sapiens RepID=Q8WVW5_HUMAN >Actin-5C n=1 Tax=Pediculus humanus corporis RepID=UPI000186E UniRef100_P confidence: 0. coverage: 2.1%num unique petot indep spec share of spectrum id's: 1.08% >Replication protein A 70 kda DNA-binding subunit n=1 Tax=Homo sapiens RepID=RFA1_HUMAN Length: 616aa 18 UniRef100_P16159 UniRef100_P confidence: 0. max coverage:num unique petot indep spec share of spectrum id's: 0.36% >Alpha-amylase/trypsin inhibitor CM16 n=2 Tax=Triticum aestivum RepID=IAC16_WHEAT >Alpha-amylase/trypsin inhibitor CMb n=2 Tax=Hordeum vulgare RepID=IAAB_HORVU Length: 143aa 5c UniRef100_P06871 UniRef100_UPI00004A5B07 UniRef100_UPI00005A2FD6 UniRef100_UPI00005A2FD confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 2 >Cationic trypsin n=1 Tax=Canis lupus familiaris RepID=TRY1_CANFA >PREDICTED: similar to trypsinogen 7 isoform 3 n=1 Tax=Canis lupus familiaris RepID=UPI00004A5B07 >PREDICTED: similar to trypsinogen 7 isoform 5 n=1 Tax=Canis lupus familiaris RepID=UPI00005A2FD6 >PREDICTED: similar to Cationic trypsin III precursor (Pretrypsinogen III) isoform 6 n=1 Tax=Canis lupus familiaris RepID=UPI00005A2FD7 PML upper band MS results: 11 entries (5 single hits) retrieved from /home/tpp/tpp/ _bowling/int-gb_ _ltq_bowling_pml-2-sequest.prot.xml
6 * corresponds to peptide is_nondegenerate_evidence flag 2a UniRef100_C5IWV5 UniRef100_P confidence: 1. max coverage:num unique petot indep spec share of spectrum id's: 28.10% >Trypsinogen n=1 Tax=Sus scrofa RepID=C5IWV5_PIG >Trypsin n=1 Tax=Sus scrofa RepID=TRYP_PIG 2b UniRef100_P confidence: 1. coverage: 6.9%num unique petot indep spec share of spectrum id's: 3.64% >Anionic trypsin n=2 Tax=Canis lupus familiaris RepID=TRY2_CANFA Length: 247aa 2c UniRef100_P confidence: 1. coverage: 8.1%num unique petot indep spec share of spectrum id's: 0.77% >Anionic trypsin-1 n=1 Tax=Rattus norvegicus RepID=TRY1_RAT 3a UniRef100_P confidence: 1. coverage: 11.7num unique petot indep spec share of spect subsumed entries: 5 >Serum albumin n=1 Tax=Bos taurus RepID=ALBU_BOVIN Length: 607aa 4a UniRef100_P confidence: 1. coverage: 25.2num unique petot indep spec share of spect subsumed entries: 1 >Keratin, type II cytoskeletal 1 n=1 Tax=Homo sapiens RepID=K2C1_HUMAN Length: 644aa 5a UniRef100_P14625 UniRef100_Q5CAQ confidence: 1. max coverage:num unique petot indep spec share of spect subsumed entries: 1 >Endoplasmin n=1 Tax=Homo sapiens RepID=ENPL_HUMAN >Tumor rejection antigen (Gp96) 1 n=1 Tax=Homo sapiens RepID=Q5CAQ5_HUMAN Length: 803aa 7a UniRef100_P confidence: 1. coverage: 17.5num unique petot indep spec share of spectrum id's: 8.18% >Keratin, type I cytoskeletal 9 n=1 Tax=Homo sapiens RepID=K1C9_HUMAN Length: 623aa 7b UniRef100_P13645 UniRef100_UPI00017BCE7F confidence: 1. max coverage:num unique petot indep spec share of spectrum id's: 7.08% >Keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=K1C10_HUMAN >keratin, type I cytoskeletal 10 n=1 Tax=Homo sapiens RepID=UPI00017BCE7F Length: 584aa 8a UniRef100_P35579 UniRef100_P confidence: 0. max coverage:num unique petot indep spec share of spect subsumed entries: 1 >Myosin-9 n=2 Tax=Homo sapiens RepID=MYH9_HUMAN >Isoform 2 of Myosin-9 n=1 Tax=Homo sapiens RepID=P Length: 1960aa 6a UniRef100_P29590 UniRef100_P UniRef100_P UniRef100_P UniRef100_P UniRef100_P UniRef100_Q15959 UniRef100_Q9BZX7 UniRef100_Q9BZX9 UniRef100_Q9BZY0 UniRef100_Q9BZY1 UniRef100_Q9BZY2 UniRef100_Q9BZY confidence: 1max coverag num unique ptot indep speshare of specsubsumed entries: 1 >Probable transcription factor PML n=1 Tax=Homo sapiens RepID=PML_HUMAN Length: 882aa >Isoform PML-2 of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >Isoform PML-3 of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >Isoform PML-3B of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >Isoform PML-X of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >Isoform PML-5 of Probable transcription factor PML n=1 Tax=Homo sapiens RepID=P >PML protein n=1 Tax=Homo sapiens RepID=Q15959_HUMAN >Tripartite motif protein TRIM19 kappa n=1 Tax=Homo sapiens RepID=Q9BZX7_HUMAN >Tripartite motif protein TRIM19 zeta n=1 Tax=Homo sapiens RepID=Q9BZX9_HUMAN >Tripartite motif protein TRIM19 epsilon n=1 Tax=Homo sapiens RepID=Q9BZY0_HUMAN >Tripartite motif protein TRIM19 delta n=1 Tax=Homo sapiens RepID=Q9BZY1_HUMAN >Tripartite motif protein TRIM19 gamma n=1 Tax=Homo sapiens RepID=Q9BZY2_HUMAN >Tripartite motif protein TRIM19 beta n=1 Tax=Homo sapiens RepID=Q9BZY3_HUMAN 7c UniRef100_P confidence: 1. coverage: 10.4num unique petot indep spec share of spect subsumed entries: 6 >Keratin, type I cytoskeletal 14 n=1 Tax=Homo sapiens RepID=K1C14_HUMAN Length: 472aa 7d UniRef100_P confidence: 1. coverage: 10.1num unique petot indep spec share of spectrum id's: 1.39% >Keratin, type I cytoskeletal 16 n=1 Tax=Homo sapiens RepID=K1C16_HUMAN Length: 473aa
7 9 UniRef100_UPI UniRef100_UPI UniRef100_UPI00017BDB3D UniRef100_UPI00017BDB confidence: 0. max coverage:num unique petot indep spec share of spectrum id's: 1.37% >MONOCLONAL ANTIBODY MAK33 n=1 Tax=Homo sapiens RepID=UPI Length: 213aa >monoclonal antibody light chain n=1 Tax=Homo sapiens RepID=UPI >FabOX108 Light Chain Fragment n=1 Tax=Homo sapiens RepID=UPI00017BDB3D >FabOX117 Light Chain Fragment n=1 Tax=Homo sapiens RepID=UPI00017BDB42 10 UniRef100_B4DR68 UniRef100_Q12931 UniRef100_Q53FS6 UniRef100_Q53G55 UniRef100_Q8N9Z3 UniRef100_Q9BV61 UniRef100_UPI000186D confidence: 0. max coverage:num unique petot indep spec share of spectrum id's: 1.37% >cdna FLJ58608, highly similar to Heat shock protein 75 kda, mitochondrial n=1 Tax=Homo sapiens RepID=B4DR68Length: 651aa >Heat shock protein 75 kda, mitochondrial n=2 Tax=Homo sapiens RepID=TRAP1_HUMAN >TNF receptor-associated protein 1 variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53FS6_HUMAN >TNF receptor-associated protein 1 variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G55_HUMAN >cdna FLJ36025 fis, clone TESTI , highly similar to TUMOR NECROSIS FACTOR TYPE 1 RECEPTOR ASSOCIATED PROTEIN n=1 Tax=Homo sapiens RepID=Q8N9Z3_HUMAN >TRAP1 protein (Fragment) n=1 Tax=Homo sapiens RepID=Q9BV61_HUMAN >heat shock protein 75 kda, putative n=1 Tax=Pediculus humanus corporis RepID=UPI000186D UniRef100_B4DVQ0 UniRef100_B4DW52 UniRef100_B4E335 UniRef100_B4E3A4 UniRef100_P60709 UniRef100_P63261 UniRef100_Q4R4H6 UniRef100_Q53G76 UniRef100_Q53G99 UniRef100_Q53GK6 UniRef100_Q6PJ43 UniRef100_Q8WVW5 UniRef100_UPI000186E confidence: 0. max coverage:num unique petot indep spec share of spectrum id's: 0.46% >cdna FLJ58286, highly similar to Actin, cytoplasmic 2 n=1 Tax=Homo sapiens RepID=B4DVQ0_HUMAN Length: 333aa >cdna FLJ55253, highly similar to Actin, cytoplasmic 1 n=1 Tax=Homo sapiens RepID=B4DW52_HUMAN >cdna FLJ52842, highly similar to Actin, cytoplasmic 1 n=1 Tax=Homo sapiens RepID=B4E335_HUMAN >cdna FLJ57283, highly similar to Actin, cytoplasmic 2 n=1 Tax=Homo sapiens RepID=B4E3A4_HUMAN >Actin, cytoplasmic 1, N-terminally processed n=24 Tax=Amniota RepID=ACTB_HUMAN >Actin, cytoplasmic 2, N-terminally processed n=9 Tax=Tetrapoda RepID=ACTG_HUMAN >Brain cdna, clone: QflA-11253, similar to human actin, gamma 1 (ACTG1), n=1 Tax=Macaca fascicularis RepID=Q4R4H6_MACFA >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G76_HUMAN >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53G99_HUMAN >Beta actin variant (Fragment) n=1 Tax=Homo sapiens RepID=Q53GK6_HUMAN >ACTG1 protein (Fragment) n=5 Tax=Eutheria RepID=Q6PJ43_HUMAN >Putative uncharacterized protein (Fragment) n=1 Tax=Homo sapiens RepID=Q8WVW5_HUMAN >Actin-5C n=1 Tax=Pediculus humanus corporis RepID=UPI000186E470
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
ENCODE DCC Antibody Validation Document
ENCODE DCC Antibody Validation Document Date of Submission Name: Email: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description:
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