The World s Leading. One of. HPLC Columns

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1 One of The World s Leading HPLC Columns Phenomenex India Laxmi Cyber City, Ground Floor, B Block, Survey no: 0 Kondapur, Hyderabad 00 08, India Tel: Fax: indiainfo@phenomenex.com Web:

2 Explore Successful Separations/Phase Overview... Explore Robust Methods... Explore Options for Every Development Route... Luna C8(), C8(), C...8 Luna PFP()... Luna Phenyl-Hexyl...8 Luna CN (Cyano)...0 Luna NH (Amino)... Luna HILIC... Luna SCX... Axia-Packed Preparative HPLC... Fast LC... Ordering Information...8

3 The world s # HPLC column. The Luna brand of columns and media is more than just a product line from Phenomenex. It is a pledge to provide you with the highest level of satisfaction for your chromatographic goals. Every aspect of Luna products has been engineered to meet the exacting demands placed on today s chromatographers. Luna products continue to uphold the quality our customers depend on. If you have never tried Luna columns or media, this brochure will guide you through the various solutions to fit your needs. For those who use Luna products daily, thank you for making Luna columns # in the world. 008 Phenomenex, Inc. All rights reserved.

4 Explore Successful Separations Your success begins with our commitment to provide the essential solutions to HPLC separations in the Luna brand. Some of the highest quality and performance standards are incorporated into Luna products, making them an indispensable platform for all areas of HPLC. Explore Resolution with Luna Selectivies Phase selectivity has the strongest impact on overall chromatographic resolution. Choosing the optimal selectivity can drive your separation to success. Luna phases span through 0 different chemistries, each offering its own unique selectivity. Luna Phases Description Particle Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) Bonded Phase Coverage (µmole/m ) ph Stability Application Reversed Phase Normal Phase HILIC IEX Silica() Unbonded silica,, 0, 0-PREP, Non-polar compounds C Carbon ligand, Good alternative to C8 when less retention is desired C8() C8 ligand optimized for improved peak shape,, 0, 0-PREP, Great starting phase for method development C8() C8 ligand optimized for improved peak shape.,,, 0, 0-PREP, From capillary LC/MS to process scale Our most popular phase CN Versatile CN phase,, For improving the retention of polar compounds NH Rugged and reproducible NH,, Sugar alcohols, anionic or hydrogen bonding compounds Phenyl-Hexyl Phenyl phase attached to C (hexyl) ligand,, 0, 0-PREP, Unique selectivity for very polar and aromatic compounds SCX Benzene sulfonic acid, Binding Capacity: 0. meq/g Amine and polyamine containing compounds HILIC Reproducible, cross-linked diol, Increased retention and MS sensitivity of polar compounds PFP() Pentafluorophenyl with a C (propyl) linkage, Highly polar compounds, halogenated compounds and isomers Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

5 Explore Robust Methods Successful methods depend on results that can tolerate minor variations in chromatographic parameters. The base silica of Luna is % pure and meticulous care is given to quality control over all aspects of silica structure and chemistry. This ensures that Luna columns will always perform consistently, resulting in method reproducibility you can trust. Reliable Performance Almost no variation is observed among the batches of Luna. Figure shows quality control test data designed to monitor the slightest differences that may affect reproducibility - particle shape and smoothness, porosimetry, bonding consistency and ph stability. Column to Column Reproducibility The chromatograms in Figure show consistency of inertness (black) and hydrophobicity (blue) for Luna µm C8() columns from 9 different batches. Almost no variation is observed. Particle Size Surface Coverage Particle Size Distribution Carbon Load % Pore Size (x00 Å) Batch No tfigure Silica Reproducibility. Batch No: Inert Test Hydrophobicity pfigure Column-to-column reproducibility for 9 batches of Luna µm C8() Metal (ppm/0) Surface Area (x00 m /g) Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

6 Explore Options for Every Development Route Luna media is available in a wide variety of particle sizes and formats, each with different attributes that can be optimized for nearly any stage of development. Direct Connect Cartridge Holder 0 x.0 mm Cartridge Standard Cartridge Holder Fast LC-MS Methods Luna media is available in MercuryMS Cartridges and online columns for quick, cost-effective screening methods. High-Speed-Technology Luna. µm C8()-HST columns deliver highly efficient separations without the need for expensive high-pressure instruments. Develop Robust Analytical Methods Analytical HPLC columns are the most widely used format and are available in a wide variety of dimensions and particle sizes. Lab-Scale Purification Redefined Axia -packed Luna preparative columns provide industry-leading lifetimes and efficiencies. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

7 Beyond our largest preparative column dimensions, Luna phases are available in bulk quantities for HPLC purification at the process, pilot, and commercial scale. The highly reproducible manufacturing process makes scaling to large scale purification extremely straight-forward. The wide range of Luna phases provides you with the selectivity choices to optimize parameters such as retention time and resolution. Aitionally, the high surface area (00 m /g) of Luna materials gives you greater loadability than most other medias. For those challenging purifications where chromatography is the best option, the Luna family offers an excellent platform for all purification challenges. Contact your Phenomenex technical consultant at for bulk media sales. 7 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

8 Luna C8(), C8(), C Your Starting-Point for All Reversed Phase Methods Luna has found a place as one of the world s top reversed phase columns because it can help optimize two important chromatographic properties: resolution and peak shape. The high efficiencies and bonded phase surface coverage provide for sharp peaks. Whether you need a column for USP methods or just general method development, Luna C8() and C8() should be your first choice every time. Pyridine Peak Asymmetry Comparison** Luna µm C8() Supelco Supelcosil µmabz Supelco Supelcosil LC-8-BD The result: Free exposed silanols virtually eliminated by complete bonding and endcapping Sharp peak shape for good method sensitivity ph stable from. to 0.0 for over 0,000 hours Waters Spherisorb µm ODS() Agilent Technologies ZORBAX µm SB-C8 GL Sciences Inertsil ODS() Pyridine and phenol elution order reversed Pyridine adsorption - no elution Waters Symmetry µm C8 0 Peak Symmetry(A S ) Comparison of 7 different μm reversed phase columns. This survey measures the degree of silanol activity on the surface of each silica. In this survey, Luna µm C8() material demonstrates the lowest silanol activity. Peak Asymmetry Comparison of Competing Columns** Phenomenex Luna μm C8() Pyridine Asymmetry A s = min App ID Waters Symmetry μm C8 Pyridine Asymmetry: A s min Agilent Technologies Zorbax μm SB-C8 Pyridine Adsorption: No elution min Conditions for all columns Mobile Phase: Acetonitrile/Water (0:0) Flow Rate:.0 ml/min Detection: nm Sample:. Pyridine. Phenol. Toluene Agilent Technologies and ZORBAX are registered trademarks of Agilent Technologies. Inertsil is a registered trademark of GL Sciences. Supelcosil is a trademark of Supelco. Waters and Symmetry are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Agilent Technologies or Waters Corporation. The comparative data presented here may not be representative for all applications. 8 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

9 Applications Polar, Acidic Drugs Phenomenex Luna µm C8() Waters Symmetry. µm C8 Conditions same for both columns Dimension: 7 x. mm Mobile Phase: 0 mm KH PO / Acetonitrile(70:0) Flow Rate: 0.7 ml/min Detection: 0 nm Sample:. Tolmetin. Naproxen. Diflunisal. Fenoprofen. Indomethacin. Ibuprofen Hydrophobic, Acidic Compounds Phenomenex Luna µm C8() Thermo Hypersil-Keystone HyPURITY Elite µm C8 Conditions same for both columns Mobile Phase: 0 mm Potassium phosphate, ph. / Acetonitrile (7:) Flow Rate:. ml/min Temperature: 0 C Detection: nm Sample:. p-hydroxybenzoic acid. Sorbic acid*. Benzoic acid*. Salicylic acid. p-toluic acid * Sorbic acid and Benzoic acid co-elute on HyPURITY Elite Basic Compounds Phenomenex Luna µm C8() Macherey Nagel Nucleosil µm C8 α- and β-acids in Hop Extract App ID App ID 7 App ID App ID 90 App ID 90 App ID App ID Conditions same for both columns Mobile Phase: 0 mm Potassium phosphate, ph. / Acetonitrile (7:) Flow Rate:. ml/min Temperature: 0 C Detection: 0 nm Sample:. Maleic acid. Triprolidine*. Chlorpheniramine*. Diphenhydramine* *Peaks - adsorb on Nucleosil C8 Column: Luna µm C8() Dimension: 0 x. mm Part No.: 00G--E0 Mobile Phase: Methanol with 0. % H P0 / Water with 0. % H P0 (90:0) Flow Rate:. ml/min Temperature: 0 C Detection: nm Sample:. Cohumulone. Ad-+humulone. Colupulone. Ad-+lupulone HyPURITY is a trademark of Thermo Hypersil-Keystone. Thermo Hypersil-Keystone is a registered trademark of Thermo Hypersil-Keystone. Waters and Symmetry are registered trademarks of Waters Corporation. Machery Nagel and Nucleosil are registered trademarks of Machery-Nagel. Phenomenex is not affiliated with any of the above companies. Comparative separations may not be representative of all applications. 9 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

10 Applications usp method: estradiol Cocaine-narcotic drug Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: Acetontrile/Water (:) Flow Rate: ml/min Temperature: 0 C Detection: nm Sample:. Ethylparaben. Estrone. Estradiol mau 00 Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: Phosphate Buffer, ph./ Acetonitrile (7:) Flow Rate: ml/min Temperature: 0 C Detection: nm Sample:. Cocaine hydrochloride App ID App ID 0 0 min min usp method: phenylephrine hydrochloride injection usp method: Hydrocortisone cream Column: Luna µm C8() Dimension: 0 x. mm Part No.: 00G--E0 Mobile Phase: Water/Methanol with. % -Octanesulfonic acid ph.0 (0:0) Flow Rate: ml/min Temperature: C Detection: 80 nm Sample:. Phenylephrine hydrochloride. Epinephrine bitartrate Column: Luna µm C8() Dimension: 0 x. mm Part No.: 00G--E0 Mobile Phase: Water/Acetonitrile (7:) Flow Rate: ml/min Temperature: 0 C Detection: nm Sample:. Hydrocortisone App ID App ID 7 8 min 0 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

11 Applications EPA 80 - Explosives EPA 80 - Polynuclear aromatic hydrocarbons (pah s) App ID Column: Luna µm C8() Dimension: 0 x. mm Part No.: 00G--E0 Mobile Phase: Methanol/Water (:) Flow Rate: ml/min Temperature: C Detection: nm Sample:. HMX. RDX.,,-Trinitrobenzene.,-Dinitrobenzene. Tetryl. Nitrobenzene 7.,,-Trinitrotoluene 8.-Amino-,-Dinitriotoluen 9. -Amino-,-Dinitrotoluene 0.,-Dinitrotoluene.,-Dinitrotoluene. -Nitrotoluene. -Nitrotoluene. -Nitrotoluene App ID 9 Column: Luna µm C8() Dimension: 0 x. mm Part No.: 00G--E0 Mobile Phase: A: Water B: Acetonitrile Gradient: A/B (:7) to 00 % B in min Flow Rate: ml/min Temperature: C Detection: nm Sample:. Naphthalene. Acenaphthalene. Fluorene. Phenanthrene. Anthracene. Fluoranthracene 7. Pyrene 8. Benzo(a)anthracene 9. Chrysene 0.Benzo(e)pyrene. Benzo(b)fluoranthene. Benzo(k)fluoranthene. Benzo(a)pyrene. Dibenzo(a,h)anthracene. Benzo(g,h,i)perylene. Indenol(,,-c,d)pyrene Ginger Pungents Cold Medicine m App ID 90 Column: Luna µm C8() Dimension: 0 x. mm Part No.: 00G--E0 Mobile Phase: A: Water B: Acetonitrile Gradient: A/B (:) to A/B (0:0) in 8 min, A/B (:) in min, A/B (0:90) in 0 min Flow Rate: ml/min Temperature: 0 C Detection: 8 nm Sample:. -Gingerol. 8-Gingerol + isomer. -Shogaol. 0-Gingerol. 8-Shogaol. 0-Shogaol mau App ID 78 Column: Luna µm C8() Part No.: 00F-9-E0 Mobile Phase: Methanol/Acetonitrile with 0. % H PO / Water with 0. % H PO and 0.% Heptane Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Acetaminophen. Pseudoephedrine. Benzoic acid. Chlorpheniramine. Dextromethorphan min Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

12 Applications Water Soluble Vitamins usp method: lorazepam tablets App ID 0 Column: Luna μm C8() Part No.: 00F--E0 Mobile Phase: 0 mm Potassium Phosphate, ph.0/acetonitrile (9:) Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Thiamine. Cyanocobalamin (Vitamin B). Ascorbic acid. Pantothenic acid. Niacinamide. p-aminobenzoic acid App ID Column: Luna μm C8() Dimension: 0 x. mm Part No.: 00G--E0 Mobile Phase: Water/Methanol/Acetic acid (::) Flow Rate: ml/min Temperature: C Detection: nm Sample:. Lorazepam min pharmaceutical PRESERVATIVES usp method: minoxidil mau App ID Column: Luna μm C Part No.: 00F-0-E0 Mobile Phase: A: 0. % Acetic acid in water/ Acetonitrile (80:0) B: 0. % Acetic acid in water/ Acetonitrile (0:80) Gradient: A/B (00:0) to A/B (0:00) in 0 min Flow Rate: ml/min Temperature: C Detection: nm Sample:. Propylparaben impurity. Benzyl alcohol. Phenol. Benzoic acid. Methylparaben. Benzaldehyde 7. Ethylparaben 8. Propylparaben App ID Column: Luna μm C8() Dimension: 0 x. mm Part No.: 00G--E0 Mobile Phase: Methanol/Water/Acetic acid with 7 mm Docusate sodium ph.0 (9.:9.7:) Flow Rate: ml/min Temperature: C Detection: nm Sample:. Medroxyprogesterone acetate. Minoxidil min min Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

13 Applications usp method: impiramine fatty acids 7 App ID Column: Luna μm C8() Dimension: 0 x. mm Part No.: 00G--E0 Mobile Phase: 0.0 M Sodium perchlorate, ph.0/acetonitrile/triethylamine (.:7.:0.) Flow Rate:. ml/min Temperature: C Detection: 9 nm Sample:. Imipramine. Desipramine min App ID 8 Column: Luna μm C8() Part No.: 00F-9-E0 Mobile Phase: A: Acetonitrile B: Water (8 Mohms DI) Gradient: A/B (70:0)) to A/B (90:0) in 0 min, A/B (90:0) to A/B (70:0) in min, hold for min Flow Rate: 0. ml/min Detection: Evaporative Light Scattering (ELSD) Temperature: C Sample:. Lauric acid. Myristic acid. Palmitoleic acid. Linoleic acid. Palmitic acid. Oleic acid 7. Heptadecanoic acid 8. Stearic acid usp method: naproxen tablets usp method: albuterol tablets Column: Luna μm C8() Part No.: 00F--E0 Mobile Phase: Acetonitrile/Water/Glacial acid, ph.0 (0:9:) Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Naproxen. Butyrophenone Column: Luna μm C8() Part No.: 00F--E0 Mobile Phase: Methanol/Water with mm Hexane sulfonic acid and % Glacial acetic acid (0:0) Flow Rate:. ml/min Temperature: C Detection: 7 nm Sample:. Albuterol App ID 8 App ID 0 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

14 Applications antioxidants Narcotics App ID 8 Column: Luna µm C8() Dimension: 00 x. mm Part No.: 00D--E0 Mobile Phase: A: Acetonitrile B: Phosphate Buffer Gradient: A/B (0:70) to A/B (70:0) in min Flow Rate: ml/min Temperature: C Detection: nm Sample:. PG. TBHQ. DMT. BHA. BHT 7 8 App ID 8099 Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: A: 0mM NHOAc, ph. B: Acetonitrile Gradient: A/B (9:) for minutes, then A/B (9:) to A/B (0:0) in minutes Flow Rate:.0 ml/min Temperature: C Detection: nm Sample:. Normorphine. Morphine. Hydromorphone. Norcodeine. Codeine. Hydrocodone 7. Cocaine 8. Norcocaine min 0 0 min Steroids App ID Column: Luna µm C8() Part No.: 00F-9-E0 Mobile Phase: H PO in Water/Acetonitrile/Methanol (::) Flow Rate:.0 ml/min Temperature: Ambient Detection: nm Sample:. Hydrocortisone. Corticosterone. -a-hydroxyprogesterone. Cortisone acetate. -Ketoprogesterone USP Method: Loratadine mau Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: A: 0.9 g -pentaesulfonic acid sodium salt in L buffered to.00 with Phosphoric Acid B: Acetonitrile Gradient: Time (min) Pct B Flow Rate:. ml/min Temperature: Ambient Detection: nm Sample:. Loratadine Related Compound A. Loratadine Related Compound B. Loratadine App ID min min Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

15 Applications Saw Palmeto Berry, p-bromophenacyl Esters USP Method: Acetaminophen App ID 07 Column: Luna µm C8() Part No.: 00F-8-E0 Mobile Phase: Acetonitrile/Water (87:) Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Capric acid. Lauric acid. Linolenic acid. Myristic acid. Linoleic acid. Palmitic acid 7. Oleic acid 8. Stearic acid Absorbance Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: Water/Methanol/Acetic Acid (9:8:) Flow Rate:. ml/min Temperature: C Detection: 7 nm Sample:. Acetaminophen. Caffeine. Benzoic Acid App ID min Cyclosporin - Immunosuppressants Capsaicin App ID 08 Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: Acetonitrile/Water, ph. w/ mm H PO (70:0) Flow Rate:. ml/min Temperature: 7 C Detection: 0 nm Sample:. Cyclosporin A. Cyclosporin D App ID 07 Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: A: Acetonitrile/Water (:) B: Acetonitrile/Water (0:0) Gradient: 00 % A in min to 00 % B in 9 min Flow Rate:. ml/min Temperature: 7 C Detection: 7 nm Sample:. Nordihydrocapsaicin. Capsaicin. Dihydrocapsaicin. Homocapsaicin min min Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

16 Luna PFP() Powerful Selectivity for Reversed Phase Methods Luna PFP() columns provide unique selectivity for highly polar compounds, complex natural products, isomers and other closely related compounds. This is achieved by using a propyl-linked pentafluorophenyl, which provides multiple retention mechanisms unique to typical reversed phase medias. Halogens can radically increase the polarity of compounds, thus decreasing typical retention characteristics. Luna PFP() columns retain, discriminate, and separate halogens easily. Luna PFP() selectivity is achieved through mechanisms of interaction Positional Isomers of Halogenated Phenols Luna µm PFP() Hydrogen Bonding Dipole-Dipole Interactions Aromatic and π-π Interactions Hydrophobic min 9! baseline separation App ID 9 Column: Luna µm PFP() Part No.: 00F-7-E0 Mobile Phase: A: 0. % Formic acid in Water B: 0. % Formic acid in Acetonitrile Gradient: A/B (0:0) to (0:0) in 0 min Flow Rate: ml/min Temperature: C Detection: nm Sample:.,-Dimethylphenol.,-Dimethylphenol.,-Dimethylphenol.,-Dimethylphenol.,-Dimethylphenol.,-Dichlorophenol 7.,-Dichlorophenol 8.,-Dichlorophenol 9.,-Dichlorophenol 0.,-Dibromophenol Luna µm C8() Achieve unique selectivity using four mechanisms of solute/stationary phase interactions Extremely discerning for halogenated, aromatic, and conjugated compounds Provides orthogonal selectivity, even using traditional reversed phase solutions min & 0! coelution App ID 97 Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: A: 0.% Formic acid in Water B: 0.% Formic acid in Acetonitrile Gradient: A/B (0:0) to (0:0) in 0 min Flow Rate: ml/min Temperature: C Detection: nm Sample:.,-Dimethylphenol.,-Dimethylphenol.,-Dimethylphenol.,-Dimethylphenol.,-Dimethylphenol.,-Dichlorophenol 7.,-Dichlorophenol 8.,-Dichlorophenol 9.,-Dichlorophenol 0.,-Dibromophenol Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

17 Isomeric Compounds Positional changes on an analyte of interest may effect the compound s dipole moment. This change can be readily noticed by the highly electronegative fluorine (F) atom and other retention mechanisms. Aromatic Compounds Aromatic compounds show unique retention characteristics on Luna PFP() compared to traditional reversed phase columns. The presence of the aromatic benzene ring in Luna PFP() increases the relative attraction between the stationary phase and aromatic analytes, leading to increased retention for these types of compounds. Excellent choice for positional isomers due to the multiple retention characteristics of Luna PFP() columns. Positional Isomers of Methylacetophenon Luna µm PFP()! mechanisms of interaction provide good resolution of isomers App ID 98 Column: Luna µm PFP() Part No.: 00F-7-E0 Mobile Phase: Water/ Methanol (0:0) Flow Rate: ml/min Temperature: C Detection: nm Sample:. o-methylacetophenone. m-methylacetophenone. p-methylacetophenone Closely related polyphenolic compounds are readily separated with Luna PFP() columns. Catechins Luna µm PFP() 7 8! baseline resolution App ID 9 Column: Luna µm PFP() Part No.: 00F-7-E0 Mobile Phase: A: 0. % Formic acid in Water B: 0. % Formic acid in Acetonitrile Gradient: A/B (80:0) to (:) in 0 min Flow Rate: ml/min Temperature: C Detection: 80 nm Sample:. Gallic acid. Epigallo catechin. Catechin. Epicatechin. Epigallocatechin gallate. Gallocatechin gallate 7. Epicatechin gallate 8. Catechin gallate min min Luna µm C8() Luna µm C8()! mechanism of interaction unable to resolve isomers App ID 99 Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: Water/ Methanol (0:0) Flow Rate: ml/min Temperature: C Detection: nm Sample:. o-methylacetophenone. m-methylacetophenone. p-methylacetophenone App ID 9 Column: Luna µm C8() Part No.: 00F--E0 Mobile Phase: A: 0. % Formic acid in Water B: 0. % Formic acid in Acetonitrile Gradient: A/B (80:0) to (:) in 0 min Flow Rate: ml/min Temperature: C Detection: 80 nm Sample:. Gallic acid. Epigallo catechin. Catechin. Epicatechin. Epigallocatechin gallate. Gallocatechin gallate 7. Epicatechin gallate 8. Catechin gallate min min 7 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

18 Luna Phenyl-Hexyl Engineered for Stability Luna Phenyl-Hexyl is a reproducible, extremely stable phenyl phase. Most other phenyl phases use a short propyl ( carbon) linker, which limits the phase stability. This Luna phase uses a hexyl ( carbon) linker to attach the phenyl group to the silica surface. The result: Highly reproducible phenyl phase Dual selectivity of both phenyl phase and a short alkyl phase (such as a C8) Excellent retention of amine and polar aromatic compounds. to 0 ph stability for 0,000 hours Antibacterials: Comparison of Phenyl Columns** Phenomenex Luna µm Phenyl-Hexyl 7 8 App ID min Conditions same for all columns Mobile Phase: A: 0 mm KH PO, ph. B: Acetonitrile Gradient: A/B (80:0) to A/B (7:) in min, then to A/B (:) in min Flow Rate:.0 ml/min Detection: nm Temperature: C Sample:. Carbadox. Thiamphenicol. Furazolidone. Oxolinic acid. Sulfadimethoxine. Sulfaquinoxaline 7. Nalidixic acid 8. Piromidic acid Waters Spherisorb µm Phenyl Agilent Technologies Zorbax µm SB-Phenyl Agilent Technologies Zorbax µm Phenyl,,, Peaks, - Poor resolution 7 Peaks,, - Poor Resolution Peaks 7,8 Some absorption App ID 08, 8 App ID 08 Peaks 7,8 - Total absorption App ID min min min Agilent Technologies and ZORBAX are registered trademarks of Agilent Technologies. Spherisorb and Waters are registered trademarks of Waters Corp. Phenomenex is not affiliated with Agilent Technologies or Waters Corp. Comparative separations may not be representative of all applications. 8 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

19 Applications cough and cold-usp Method App ID 7 Column: Luna µm Phenyl-Hexyl Part No.: 00F-7-E0 Mobile Phase: Methanol/Water with 0. % H PO and 0. % Heptane Sulfonate/ Acetonitrile with 0. % H PO (::0) Flow Rate:.0 ml/min Detection: nm Temperature: C Sample:. Acetaminophen. Pseudoephedrine. Chlorphenaramine. Dextromethorphan food aitives App ID 99 Column: Luna µm Phenyl-Hexyl Part No.: 00F-7-E0 Mobile Phase: A: 0 mm KH PO + 0.% H PO B: Acetonitrile Gradient: A/B (7:) to A/B (:7) in 8 min, hold at A/B (:7) for min Flow Rate:.0 ml/min Detection: 0 nm Temperature: C Injection: 0 µl Sample:. Saccharin. p-hydroxybenzoic acid. Sorbic acid. p-hydroxbenzoic acid methyl ester. Dehydroacetic acid. p-toluic acid 7. p-hydroxybenzoic acid ethyl ester 8. n-propyl p-hydroxybenzoate ChLorobenzenes Column: Luna µm Phenyl-Hexyl cough and cold medicine Part No.: 00F-7-E0 Mobile Phase: A: Water B: Acetonitrile Gradient: A/B (0:0) to A/B (:) in 0 min Flow Rate:.0 ml/min Detection: nm Temperature: C Sample:. Chlorobenzene.,-Dichlorobenzene mau.,-dichlorobenzene.,,-trichlorobenzene 0.,,-Trichlorobenzene.,,,-Tetrachlorobenzene Column: Luna µm Phenyl-Hexyl Dimension: 7 x. mm Part No.: 00C--E0 Mobile Phase: A: Acetonitrile B: 0 mm KH PO / Methanol(80:0) ph 9.0 Gradient: A/B (0:00) to A/B (80:0) in min Flow Rate:.0 ml/min Detection: nm Temperature: C Injection: 0 µl Sample:. p-aminophenol. Benzoic acid. Acetaminophen. Pseudoephedrine. Butyl paraben. Chlorphenaramine 7. Diphenhydramine 8. Dextromethorphan App ID App ID Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

20 Luna (CN) Cyano Proven Reproducibility Luna CN columns were developed to provide reproducibile chromatography from run-to-run, column-to-column and batch-tobatch. Luna high-purity silica provides a ridged and dense column bed that allows for improved CN bonding techniques to make a stable CN phase. The result: One of the most stable CN columns under both reversed phase or normal phase conditions. Reproducible from run-to-run, column-to-column, batch-to-batch. ph stable from. to 7.0 Batch-to-Batch Reproducibility Batch Batch Batch Column: Luna µm CN Mobile Phase: A: Hexane, B: Methylene chloride/ Methanol(80:0), A/B (80:0) Flow Rate:.0 ml/min Detection: nm Injection:.0 µl Temperature: Ambient Sample:. Hydrocortisone. Prednisone. Cortisone. Hydrocortisone acetate App ID Column-to-Column Reproducibility Column: Luna µm CN Mobile Phase: A: Hexane, B: Methylene chloride/methanol(80/0), A/B (9:) Flow Rate:.0 ml/min Detection: nm Injection:.0 µl Temperature: Ambient Sample:. Dimethyl phthalate. Diethyl phthalate. Dibutyl phthalate. Dioctyl phthalate Column Column Column App ID Phthalate Esters: A Comparsion of CN Columns** Phenomenex Luna µm CN Waters Spherisorb µm CN min App ID min Agilent Technologies Zorbax µm SB-CN min Conditions same for all columns Mobile Phase: A: Hexane, B: Methylene chloride/methanol (80:0), A/B (99:) Flow Rate:.0 ml/min Detection: nm Temperature: Ambient Sample:. Di-n-octyl phthalate. Bis (-Ethylhexyl) phthalate. Butylbenzyl phthalate. Di-n-butyl phthalate. Diethyl phthalate. Dimethyl phthalate Agilent Technologies and ZORBAX are registered trademarks of Agilent Technologies. Waters and Spherisorb are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Agilent Technologies or Waters Corporation. The comparative data presented here may not be representative for all applications. 0 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

21 Luna (NH ) Amino Developed for Ruggedness Luna NH columns were developed to provide improved amino column lifetime. Column life for most amino columns can be problematic as the amino bonding easily strips off the silica. Luna NH columns, however, show good bonded phase stability under both normal and reversed phase modes and across a ph range of. to.0. Such a broad ph range indicates the bonded phase ruggedness and the density of the bonded phase coverage. The result: Long lifetimes and low phase bleed for more reproducible methods Excellent retention of simple sugars, complex sugars, sugar alcohols by reverse phase conditions and hydrogen bonding compounds under normal phase conditions ph stable from. to.0 Stable in 00 % aqueous mobile phases NUCLEIC ACID BASES min APP ID 0 Column: Luna µm NH Part No.: 00F-78-E0 Mobile Phase: Acetonitrile/Water (80:0) Flow Rate:.0 ml/min Detection: nm Temperature: 0 C Sample:. Thymine. Uracil. Cytosine. Adenine. Guanosine Simple Sugars Column: Luna µm NH Dimension: 0 x. mm Part No.: 00G-78-E0 Mobile Phase: Acetonitrile/Water (80:0) Flow Rate:.0 ml/min Detection: RI Temperature: 0 C Sample:. Xylose. Fructose. Glucose. Sucrose. Maltose. Melezitose 7. Raffinose Steroids Column: Luna µm NH Dimension: 0 x. mm Part No.: 00G-78-E0 Mobile Phase: Hexane/Ethanol (8:) Flow Rate:.0 ml/min Detection: 0 nm Temperature: C Sample:. -Ketoprogesterone. -Hydroxyprogesterone. Cortisone acetate. Prednisolone -acetate. Cortisone. Prednisolone 7 APP ID 8 APP ID min min Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

22 Luna HILIC Increase MS-Sensitivity and Retention for Polar Compounds Luna HILIC columns retain a water-enriched layer on the surface of the silica. This water layer facilitates the transfer of polar compounds into the stationary phase for increased retention. The result: Superior retention of polar compounds Improve mass spec sensitivity Increased laboratory throughput and productivity Separation is achieved through the partitioning of polar solutes from the high concentration, water-miscible, organic mobile phase into the hydrophilic surface environment. Polar solutes exhibit increased retention, and elute in the order of increasing hydrophilicity. Improve Mass Spec Sensitivity Luna HILIC columns allow low level polar metabolites to be retained on column past the critical ion suppression zone, allowing: Increased MS sensitivity and Higher signal-to-noise ratio (S/N). S/N = 0. Polar Compound in HILC Mode Primary Ion Suppression Region Great MS sensitivity Column: Luna µm HILIC Dimension: 00 x.0 mm Part No.: 00D-9-B0 Mobile Phase: Acetonitrile / 00 mm Ammonium Formate, ph. (90:0) Flow Rate: 0. ml/min Detection: Mass Spectrometer (MS) Temperature: Ambient Sample: Bamethan App ID 0 t o min Poor MS sensitivity S/N =. App ID Polar Compound in C8 Reversed Phase Column: Gemini µm C8 Dimension: 00 x.0 mm Part No.: 00D--B0 Mobile Phase: 0. % Formic Acid / Acetonitrile (97:) Flow Rate: 0. ml/min Detection: Mass Spectrometer (MS) Temperature: Ambient Sample: Bamethan Ion Suppression Region is from min t o = ~ 0. min t k'== R t o - 0. = t o 0. 0 min t o Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

23 Retain Polar Compounds Highly polar compounds such as ribavirin may be poorly retained on reversed phase columns. HILIC techniques will increase polar compound retention and sensitivity. Unique HILIC Selectivity Not all HILIC columns are alike. Luna HILIC columns deliver on the exacting standards you ve come to trust from the Luna product line. Ribavirin on Luna HILIC HILIC Column Comparison** 0. ng on column Increased retention and sensitivity Peak area = 0,79 Requires gradient to elute App ID Column: Luna µm HILIC Dimension: 00 x.0 mm Part No.: 00D-9-B0 Mobile Phase: A: Acetonitrile/00 mm Ammonium Formate, ph. (90:0) B: Acetonitrile/0 mm Ammonium Formate, ph. (0:0) Gradient: 00 % A for min, then 00 % B to. min, switch to 00 % A for 0 min Flow Rate: 0. ml/min Detection: Mass Spectrometer (MS) Temperature: Ambient Sample:. Ribavirin (MRM:./.) Phenomenex Luna µm HILIC to Baseline resolution of all peaks min App ID min Waters Atlantis μm HILIC Ribavirin on C8 0. ng on column to (Thiamine-no elution) App ID k = 0.8 Elutes at void Peak area = 7, min App ID Column: Gemini µm C8 Dimension: 00 x.0 mm Part No.: 00D--B0 Mobile Phase: Acetonitrile with 0. % v/v Formic Acid/ Water with 0. v/v % Formic Acid (:97) Flow Rate: 0. ml/min Detection: Mass Spectrometer (MS) (ambient) Temperature: Ambient Sample:. Ribavirin (MRM:./.) min SeQuant μm ZIC -HILIC to min (Thiamine-no elution) App ID Conditions same for all columns: Column: As noted Mobile Phase: Acetonitrile/00 mm Ammonium Formate, ph. (90:0) Flow Rate:.0 ml/min Detection: 0 nm Sample:. PABA. Nicotinamide. Riboflavin. Nicotinic Acid. Pyridoxine. Thiamine ZIC is a registered trademark of Merck SeQuant AB. Waters and Atlantis are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Merck SeQuant AB or Waters Corporation. The comparative data presented here may not be representative for all applications. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

24 Luna SCX Develop Robust Strong Cation Exhange Methods Luna SCX columns provide excellent resolution and peak shape of basic, cationic compounds. However, most SCX columns show poor peak shape and bad resolution causing many chromatographers to ignore this important phase for small molecule method development, until now. Luna SCX columns contain a benzene sulfonic acid ligand providing ion exchange reversed phase, and aromatic interactions. In combination with the ultra pure silica, Luna SCX columns are a stable, robust phase for strong cation exchange chromatography. The result: Resolving power and sharp peak shape to separate complex cationic/basic and nitrogen containing compounds Benzene sulfonic acid ligand provides mixed-mode interaction improving separation for D peptide applications Excellent first dimension of D LC applications Peptides App ID 7 mi n Column: Luna μm SCX Part No.: 00F-98-E0 Mobile Phase: A: 0 mm Potassium Phosphate, % Acetonitrile, ph. B: 0 mm Potassium Phosphate, % Acetonitrile, 00 mm Potassium Chloride, ph. Gradient: A/B (9:) to A/B (0:90) in minute Flow Rate: ml/min Temperature: C Detection: nm Injection Volume: µl ( µg on column) Sample: Peptide Mixture - Substance P. Fragment - (+). Fragment - (+). Fragment - (+). Fragment -9 (+). Intact (+). (ARG-PHE-TRP-LEU) (+) Tryptic Digest of Bovine Cytochrome C min App ID 77 Column: Luna μm SCX Part No.: 00F-98-E0 Mobile Phase: A: 0 mm Potassium Phosphate, ph. / % Acetonitrile B: 0 mm Potassium Phosphate, ph. / % Acetonitrile / 0 mm Potassium Chloride Gradient: 00 % A to 00 % B in 0 minutes Flow Rate: ml/min Temperature: C Detection: nm Injection Volume: 0 µl (0 µg on column) Sample: Bovine Cytochrome C trypsin digest Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

25 AXIA Revolutionize Lab-Scale Purification An advanced column packing and hardware design, Axia columns incorporate patent-pending Hydraulic Piston Compression technology to eliminate bed collapse as a source of failure in preparative columns. Using a single, controlled hydraulic compression, the piston assembly is locked in place without allowing the media to decompress or relax, thus maintaining media and column bed integrity. With Axia technology, the correct slurry amount and packing pressure are automated to give not only higher efficiency and sharper peaks, but also drastically reduced column-to-column variability. This will help improve longer column lifetime, column-to-column reproducibility, and recover higher compound purity with analytical like efficiency. Axia Gradient Lifetime Study Compare Lifetime** 0000 mau Axia Packed Luna µm C8() Axia Packed 00 First Run 800th Run Overlay of, 800, 00 chromatograms Cycles: mau vs. min Leading Competitive Preparative Column Waters XTerra µm Prep MS C8 OBD First Run 0th Run* *Six columns tested. Best lifetime chosen for comparison Columns: Luna µm C8() Axia Packed Dimension: 0 x.mm Part No.: 00B--P0-AX Mobile Phase: A: 0.% TFA in Water B: 0.% TFA in Acetonitrile Gradient: Linear 9: to :9 (A/B) over 7 min, hold min Injection Volume: 00 μl Flow Rate: 0 ml/min Temperature: Ambient Detection: nm Sample:. Triprolidine. mg. Methacycline mg. Amitriptyline. mg min 00 R&D 00 Award Recipient 0 min Waters and XTerra are registered trademarks of Waters Corporation. OBD is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. The comparative data presented here may not be representative for all applications. same for both columns except where noted Column: Luna µm C8() Axia Packed Waters XTerra µm Prep MS C8 OBD Dimension: 0 x. mm (Luna); 0 x 9 mm (XTerra ) Mobile Phase: A: 0. % TFA in Water B: 0. % TFA in Acetonitrile Gradient: Linear 9: (A/B) to :9 (A/B) over 7 min, hold min Flow Rate: 0 ml/min (Luna) ml/min (XTerra ) Temperature: Ambient Detection: nm Sample:. Triprolidine. mg. Methacycline mg. Amitriptyline. mg Same linear velocity For more detailed information on Axia Preparative columns visit: Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

26 Fast LC When you want Fast LC, you need BALANCE The ever-increasing demand for high-throughput analysis of drug candidates during the early stages of drug discovery has generated an acute need for rapid methods of analysis. Balanced Solutions to: Balance Your Speed, Pressure, Efficiency and Selectivity Speed Pressure Efficiency Selectivity High Speed Technology (HST) Columns Fast < 00 Bar Highest Several phases MercuryMS Columns and Cartridges Fast < 00 Bar High Most phases Monolithic Columns Fastest < 00 Bar Good Some phases HST Columns: % Faster. No Loss in Resolution Luna μm C8() 0 x. mm Flow Rate:. ml/min R S = min App ID 8 Developing ultra-fast and efficient methods for potential drugs has become a constant challenge for analysts. Use the chart above to determine the HPLC column that meets your performance needs. Luna μm C8() 0 x. mm Flow Rate:. ml/min Luna: High Speed Technology (HST) columns High efficiency. µm particles on ultra-pure silica Ultra-high performance results on your current HPLC Easy method transfer Orthogonal selectivity options Luna HST columns are manufactured in specific dimensions utilizing new, highly controlled and robust packing technologies. The technology allows for consistent, high performance results on newer and existing HPLC instrumentation. Get the benefit of increased speed and efficiency with standard HPLC system pressure capabilities! Luna HST can be used with your current standard HPLC and newer high performance systems so that there will be no need for time consuming method revalidation. Luna HST. µm columns allow the scientist to reduce analysis time by increasing flow rates without a loss in performance. R S = min Luna. μm C8()-HST 00 x.0 mm Flow Rate: 0. ml/min R S = 0 0 min App ID 0 App ID 7 Run time reduced by 0 min with virtually no effect on resolution! Column: Luna C8(), particle size as noted Dimension: as noted Mobile Phase: A: Water B: Acetonitrile Gradient: 90:0 (A/B) to :9 (A/B) Flow Rate: As noted Detection: 70 nm Sample: Ketones C to C Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

27 Retention time (min) or Symmetry MercuryMS : Columns and Cartridges Ultra-fast, low-cost analysis for high-throughput laboratories Packed with Luna, Synergi, and Gemini material Short 0 and 0 mm cartridge formats use a new proprietary slurry packing process Rugged Durability for Over,000 Injections When running long sequences, often involving hundreds of valuable samples, it is imperative that the column does not fail during the mile of the run. As shown, the Luna μm C8() 0 x.0 mm MercuryMS cartridge was stable for over 000 injections (over 0,000 column volumes of mobile phase) in this gradient assay, offering exceptional reliability for automated screening systems. Reduce Analysis Times by 0 % With the increasing emphasis on high sample throughput for screening combinatorial libraries as well as the need for overall faster cycle time, it has become necessary for the chromatographer to reduce analysis time while still maintaining acceptable resolution. As shown, retention times can be significantly reduced with a 0 x.0 mm MercuryMS cartridge column. MercuryMS: Stable Over,000 Injections 0% Reduction in Analysis Time Using MercuryMS 0 x. mm Column 0 min 0 x. mm Column 0 min 0 x.0 mm Cartridge 0 % Reduction in Run Time vs. vs. 0 min App ID 08 App ID 080 App ID 089 Column: Luna μm C8() Dimension: 0 x. mm, 0 x. mm, 0 x.0 mm Mobile Phase: A: 0. % Formic acid in Water B: 0. % Formic acid Acetonitrile Gradient: 9: A/B to :9 in min for 0 x. mm 9: A/B to :9 in min for 0 x. mm 9: A/B to :9 in. min for 0 x.0 mm Flow Rate: ml/min for 0 and 0 x. mm,. ml/min for 0 x.0 mm Detection: nm Temperature: Ambient Sample: µl Gradient mixture. Acetaminophen. Propranolol. Imipramine. Naproxen. Valerophenone Injection# 00 tr # tr # tr # Symmetry # Symmetry # Symmetry # Column: Luna μm C8() 0 x.0 mm MercuryMS Cartridge Part No.: 00M--B0-CE Mobile Phase: A: Water with 0. % Trifluoroacetic acid (TFA) B: Methanol with 0. % TFA Gradient: 9: A/B to : 9 A/B in min at a flow rate of 0. ml/min, then reequilibrate for min at.0 ml/min Flow Rate: 0. and.0 ml/min Detection: 0 nm Temperature: Ambient Sample: µl containing:. Propranolol. Metoprolol. Pindolol 7 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

28 Ordering Information. μm High Speed Technology (HST) Columns (mm) 0 x.0 0 x.0 00 x.0 0 x.0 00 x.0 Phases Luna. µm C8()-HST 00A--B0 00B--B0 00D--B0 00B--Y0 00D--Y0 μm and μm Capillary Columns (mm) 0 x x x x x x 0.0 Phases μm C8() 00B-8-AC 00F-8-AC 00G-8-AC 00B-8-AF 00F-8-AF 00G-8-AF μm C8() 00B--AC 00F--AC 00G--AC 00B--AF 00F--AF 00G--AF μm C8() 00B-9-AC 00F-9-AC 00G-9-AC 00B-9-AF 00F-9-AF 00G-9-AF μm C8() 00B--AC 00F--AC 00G--AC 00B--AF 00F--AF 00G--AF μm Phenyl-Hexyl 00B-7-AC 00F-7-AC 00B-7-AF 00F-7-AF MercuryMS LC/MS Cartridges (mm) 0 x.0 0 x.0 0 x.0 0 x.0 Phases Qty /ea /ea /ea /ea /ea μm C8() 00N--B0-CE 00N--D0-CE 00M--B0-CE 00M--D0-CE C8() 00N-8-B0-CE 00N-8-D0-CE 00M-8-B0-CE 00M-8-D0-CE μm C8() 00N--B0-CE 00M--B0-CE 00M--D0-CE C8() 00N-9-B0-CE 00M-9-B0-CE 00M-9-D0-CE Cartridge Holders Standard Cartridge Holders Part No. Description Price CH0-8 0 mm standard holder CH0-8 0 mm standard holder Direct-Connect Cartridge Holders Part No. Description Price CH mm direct-connect holder CH mm direct-connect holder SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-8 SecurityGuard μm Microbore and Minibore Columns (mm) Cartridges (mm) 0 x.0 0 x.0 0 x.0 0 x.0 00 x.0 0 x.0 x.0 Phases /0 pk Silica() 00A--B0 00B--B0 00D--B0 00F--B0 AJ0-7 C8() 00B-8-A0 00F-8-A0 00A-8-B0 00B-8-B0 00D-8-B0 00F-8-B0 AJ0-89 C8() 00B--A0 00F--A0 00A--B0 00B--B0 00D--B0 00F--B0 AJ0-8 CN 00A--B0 00B--B0 00D--B0 00F--B0 AJ0-0 Phenyl-Hexyl 00B--A0 00A--B0 00B--B0 00D--B0 00F--B0 AJ0-0 NH 00F-77-A0 00A-77-B0 00B-77-B0 00D-77-B0 00F-77-B0 AJ0-0 HILIC 00B-9-B0 00D-9-B0 00F-9-B0 AJ0-88 PFP() 00A-7-B0 00B-7-B0 00D-7-B0 00F-7-B0 AJ0-8 for ID:.0-.0 mm 8 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

29 Ordering Information SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-8 SecurityGuard μm Analytical Columns (mm) Cartridges (mm) 0 x.0 0 x.0 0 x.0 0 x. 0 x. 7 x. 00 x. 0 x. x.0 x.0 Phases /0 pk /0 pk Silica() 00F--Y0 00B--E0 00D--E0 00F--E0 AJ0-7 AJ0-8 C8() 00A-8-Y0 00B-8-Y0 00F-8-Y0 00A-8-E0 00B-8-E0 00C-8-E0 00D-8-E0 00F-8-E0 AJ0-89 AJ0-90 C8() 00A--Y0 00B--Y0 00F--Y0 00A--E0 00B--E0 00C--E0 00D--E0 00F--E0 AJ0-8 AJ0-87 CN 00B--Y0 00F--Y0 00A--E0 00B--E0 00C--E0 00D--E0 00F--E0 AJ0-0 AJ0-0 Phenyl-Hexyl 00B--Y0 00F--Y0 00A--E0 00B--E0 00C--E0 00D--E0 00F--E0 AJ0-0 AJ0- NH 00B-77-Y0 00F-77-Y0 00A-77-E0 00B-77-E0 00D-77-E0 00F-77-E0 AJ0-0 AJ0-0 HILIC 00B-9-Y0 00F-9-Y0 00D-9-E0 00F-9-E0 AJ0-88 AJ0-89 PFP() 00B-7-Y0 00F-7-Y0 00B-7-E0 00D-7-E0 00F-7-E0 AJ0-8 AJ0-87 for ID:.0-.0 mm.-8.0 mm SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-8 SecurityGuard μm Microbore and Minibore Columns (mm) Cartridges (mm) 0 x.0 0 x.0 0 x.0 0 x.0 0 x.0 0 x.0 0 x.0 x.0* Phases /0 pk Silica() 00B-7-B0 00F-7-B0 00G-7-B0 AJ0-7 C 00A-0-B0 00B-0-B0 00F-0-B0 AJ0-9 C8 () 00B-9-A0 00F-9-A0 00G-9-A0 00A-9-B0 00B-9-B0 00F-9-B0 00G-9-B0 AJ0-89 C8 () 00B--A0 00F--A0 00G--A0 00A--B0 00B--B0 00F--B0 00G--B0 AJ0-8 CN 00A--B0 00B--B0 00F--B0 00G--B0 AJ0-0 Phenyl-Hexyl 00B-7-A0 00F-7-A0 00G-7-A0 00A-7-B0 00B-7-B0 00F-7-B0 00G-7-B0 AJ0-0 NH 00F-78-A0 00A-78-B0 00B-78-B0 00F-78-B0 00G-78-B0 AJ0-0 PFP() 00A-8-B0 00B-8-B0 00F-8-B0 AJ0-8 for ID:.0-.0 mm Contact your Phenomenex technical consultant at for bulk media sales. SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-8 SecurityGuard μm Analytical Columns (mm) Cartridges (mm) 0 x.0 0 x.0 0 x.0 0 x.0 0 x. 0 x. 7 x. x.0* x.0* Phases /0 pk /0 pk Silica() 00B-7-E0 AJ0-7 AJ0-8 C 00F-0-Y0 00B-0-E0 AJ0-9 AJ0-9 C8() 00B-9-Y0 00F-9-Y0 00G-9-Y0 00A-9-E0 00B-9-E0 00C-9-E0 AJ0-89 AJ0-90 C8() 00A--Y0 00B--Y0 00F--Y0 00G--Y0 00A--E0 00B--E0 00C--E0 AJ0-8 AJ0-87 CN 00B--Y0 00F--Y0 00G--Y0 00A--E0 00B--E0 00C--E0 AJ0-0 AJ0-0 Phenyl-Hexyl 00A-7-Y0 00B-7-Y0 00F-7-Y0 00G-7-Y0 00A-7-E0 00B-7-E0 00C-7-E0 AJ0-0 AJ0- NH 00B-78-Y0 00F-78-Y0 00G-78-Y0 00A-78-E0 00B-78-E0 00C-78-E0 AJ0-0 AJ0-0 SCX 00B-98-E0 AJ0-07 AJ0-08 HILIC 00F-0-Y0 AJ0-88 AJ0-89 PFP() 00B-8-Y0 00F-8-Y0 00A-8-E0 00B-8-E0 AJ0-8 AJ0-87 for ID:.0-.0 mm.-8.0 mm If Luna analytical columns do not provide at least an equivalent separation as compared to a competing analytical column of the same particle size, similar phase and dimensions, return the column with comparative data within days for a FULL Refund. 9 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

30 Ordering Information μm Analytical, Semi-Prep and Preparative Columns (mm) SecurityGuard Cartridges (mm) 00 x. 0 x. 0 x. 0 x 0 0 x x.0* 0 x 0 Phases /0 pk / pk Silica() 00D-7-E0 00F-7-E0 00G-7-E0 00G-7-N0 AJ0-8 AJ0-7 C 00D-0-E0 00F-0-E0 00G-0-E0 AJ0-9 AJO-77 C8() 00D-9-E0 00F-9-E0 00G-9-E0 00G-9-N0 00G-9-AK AJ0-90 AJ0-7 C8() 00D--E0 00F--E0 00G--E0 00G--N0 00G--AK AJ0-87 AJ0-7 CN 00D--E0 00F--E0 00G--E0 00G--N0 AJ0-0 AJ0-7 Phenyl-Hexyl 00D-7-E0 00F-7-E0 00G-7-E0 00G-7-N0 AJ0- AJ0-7 NH 00D-78-E0 00F-78-E0 00G-78-E0 00G-78-N0 AJ0-0 AJ0-7 SCX 00D-98-E0 00F-98-E0 00G-98-E0 00G-98-N0 AJ0-08 AJ0-79 HILIC 00D-0-E0 00F-0-E0 00G-0-E0 AJ0-89 PFP() 00D-8-E0 00F-8-E0 00G-8-E0 00G-8-N0 AJ0-87 AJ0-87 for ID:.-8.0 mm 9- mm Luna Method Development Kits The Luna Method Development Kits combine the incredible performance of Luna HPLC columns with three different selectivities. Both kits include a Luna Phenyl-Hexyl (moderate polarity), a Luna CN (polar), and your choice of a C8() or C8() (non-polar). A wide range of selectivies for virtually any separation challenge. Method Development Kits Part No. Description Dimensions (mm) Unit Price KH0-70 Luna Method Development Kit ( columns) 0 x. /pk each of µm C8(), C8(), C, CN, Phenyl-Hexyl Phases KH0- Luna Rapid-MD Kit ( columns) 0 x. /pk each of µm C8(), C8() and Phenyl-Hexyl phases KH0-7 Luna Selectivity Kit ( columns) 0 x. /pk each of µm C8(), Phenyl-Hexyl, CN phases KH0-7 Luna Selectivity Kit ( columns) each of µm C8(), Phenyl-Hexyl, CN phases 0 x. /pk Axia Packed Preparative Columns (mm) SecurityGuard Cartridges (mm) 0 x. 00 x. 0 x. 0 x. 0 x 0 00 x 0 0 x 0 x.** x 0 Phases /ea /ea μm Silica() 00B-7-P0-AX 00D-7-P0-AX 00F-7-P0-AX 00G-7-P0-AX 00B-7-U0-AX 00D-7-U0-AX 00G-7-U0-AX AJ0-79 AJ0-8 C 00F-0-P0-AX C8() 00B-9-P0-AX 00D-9-P0-AX 00F-9-P0-AX 00B-9-U0-AX 00D-9-U0-AX AJ0-780 AJ0-80 C8() 00B--P0-AX 00D--P0-AX 00F--P0-AX 00G--P0-AX 00B--U0-AX 00D--U0-AX 00G--U0-AX AJ0-789 AJ0-80 CN 00B--P0-AX 00F--P0-AX 00G--P0-AX 00D--U0-AX AJ0-80 AJ0-8 Phenyl-Hexyl 00B-7-P0-AX 00D-7-P0-AX 00F-7-P0-AX 00B-7-U0-AX 00D-7-U0-AX AJ0-78 AJ0-80 NH 00D-78-P0-EX 00F-78-P0-AX 00G-78-P0-AX 00G-78-U0-AX AJ0-8 AJ0-809 PFP() 00B-8-P0-AX 00D-8-P0-AX 00F-8-P0-AX 00G-8-P0-AX 00B-8-U0-AX 00D-8-U0-AX 00G-8-U0-AX AJ0-877 AJ0-878 HILIC 00B-0-P0-AX 00D-0-P0-AX 00F-0-P0-AX 00G-0-P0-AX 00B-0-U0-AX 00D-0-U0-AX 00G-0-U0-AX for ID: 8-9 mm 0-9 mm 0 μm Semi-Prep SecurityGuard Cartridges (mm) 0 x. 0 x 0 x.0* 0 x 0 Phases /0 pk / pk Silica() 00G-09-E0 00G-09-N0 AJ0-8 AJ0-7 C 00G-09-E0 00G-09-N0 AJ0-9 AJ0-77 C8() 00G-0-E0 00G-0-N0 AJ0-90 AJ0-7 C8() 00G--E0 00G--N0 AJ0-87 AJ0-7 CN 00G-00-E0 00G-00-N0 AJ0-0 AJ0-7 Phenyl-Hexyl 00G-8-E0 00G-8-N0 AJ0- AJ0-7 NH 00G-79-E0 00G-79-N0 AJ0-0 AJ0-7 SCX 00G-0-E0 00G-0-N0 AJ0-08 AJ0-79 for ID:.-8.0 mm 9- mm Axia Packed Preparative Columns (mm) SecurityGuard Cartridges (mm) 0 x. 00 x. 0 x. 0 x. 0 x 0 00 x 0 0 x 0 0 x 0 0 x 0 00 x 0 x.** x 0 Phases /ea /ea 0 μm Silica() 00G-09-P0-AX 00G-09-U0-AX AJ0-79 AJ0-8 C 00G-09-P0-AX 00G-09-V0-AX 00D-09-V0-AX C8() 00B-0-P0-AX 00F-0-P0-AX 00G-0-P0-AX 00B-0-U0-AX 00D-0-U0-AX 00G-0-U0-AX 00G-0-V0-AX 00D-0-V0-AX AJ0-780 AJ0-80 C8() 00B--P0-AX 00D--P0-AX 00F--P0-AX 00G--P0-AX 00B--U0-AX 00D--U0-AX 00G--U0-AX 00G--V0-AX 00B--V0-AX 00D--V0-AX AJ0-789 AJ0-80 CN 00G-00-P0-AX AJ0-80 AJ0-8 Phenyl-Hexyl 00G-8-P0-AX 00G-8-U0-AX 00B-8-V0-AX AJ0-78 AJ0-80 NH 00D-79-P0-AX 00G-79-P0-AX AJ0-8 AJ0-809 for ID: 8-9 mm 0-9 mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-8 SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-70 **PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8 PREP SecurityGuard Cartridges require holder, Part No.: AJ Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

31 Phenex Syringe Filters For Sample and Solvent Filtration Prior to Chromatography! Less system down time More consistent, reproducible results Increased column lifetime mm Diameter for ml sample volumes - 7 mm Diameter for 0 ml sample volumes - 8 mm Diameter for 0 00 ml sample volumes Membrane Type/Size Part No. Unit Price Part No. Unit Price Part No. Unit Price 0.0 µm Phenex-RC AF /pk AF /pk AF /pk (Regenerated Cellulose) AF /pk AF /Pk AF /pk Phenex-PES AF /pk (Polyethersulfone) AF /pk Phenex-PTFE AF /pk AF /pk AF /pk (Polytetrafluoroethylene) AF /pk AF /pk AF /pk Phenex-NY AF-07-00/pk AF /pk AF /pk (Nylon) AF-07-00/pk AF /pk AF /pk Phenex-GF/NY An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF0-A7-00/pk (Glass Fiber/Nylon) (NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages, environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most AF0-A7-00/pk difficult samples. Outlet connection is luer-lock. Phenex-GF/CA,, (Glass Fiber/Cellulose Acetate) 0. µm Phenex-RC (Regenerated Cellulose) Phenex-PES (Polyethersulfone) Phenex-PTFE (Polytetrafluoroethylene) Phenex-NY (Nylon) Phenex-GF/NY (Glass Fiber/Nylon) Phenex-GF/CA,, (Glass Fiber/Cellulose Acetate).0 µm Phenex-GF, (Glass Fiber) An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. Excellent for filtration of tissue culture media, general biological sample filtration and clarification. Outlet connection is luer lock. AF0-8A09-7 AF0-8A /pk 00/pk AF /pk AF /pk AF /pk AF /pk AF /Pk AF /pk AF-08-00/pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF /pk AF-07-00/pk AF /pk AF /pk AF-07-00/pk AF /pk AF /pk An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF0-B7-00/pk (NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages, AF0-B7- environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most 00/pk difficult samples. Outlet connection is luer-lock. An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA AF0-8B /pk membrane. Excellent for filtration of tissue culture media, general biological sample filtration and clarification. Outlet connection is luer lock. AF0-8B /pk Prefiltration of heavily contaminated or highly viscous samples. When used in-series preceding a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. Outlet connection is luer lock. AF AF /pk 00/pk Phenex Offers:»» Maximum chemical compatibility»» Minimum extractables»» Excellent flow rate»» High total throughput»» Certified quality Tip: Try a Sample Pack! The best way to determine if a specific Phenex membrane is suitable for your application. Request yours today by phone or visit 00 % integrity tested»» Low hold-up volume»» Low protein absorption»» Bi-directional use Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP), and offer luer lock inlet/slip outlet connections, unless otherwise indicated.. 7 mm diameter.. Glass fiber filters are 8 mm diameter and made of borosilicate. They will remove 90 % of all particles >. µm.. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cyrolite.. Cellulose acetate is surfactant-free.. mm diameter.. Hydrophobic membrane. Can be made hydrophilic by prewetting with IPA mm diameter. 8. Aitional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance. 9. Larger quantity purchases at significant savings are available. If Phenex Syringe Filters do not perform as well or better than your current syringe filter product of similar membrane, diameter and pore size, send in your comparative data within days for a FULL REFUND. Terms and Conditions Phenomenex reserves the right to refuse sale at its discretion. Subject to Phenomenex Standard Terms & Conditions, which may be viewed at Trademarks SecurityGuard, Luna, Gemini, AXIA, Phenex, MercuryMS, and Synergi are trademarks of Phenomenex, Inc. ZORBAX is a registered trademark of Agilent Technologies. Symmetry, Spherisorb, Atlantis, and XTerra are registered trademarks of Waters Corp. Supelcosil is a trademark of Sigma-Aldrich Biotechnology. Nucleosil is a registered trademark of Macherey-Nagel. HyPURITY is a trademark of Thermo Electron Corporation. Cryolite is a trademark of CY/RO Industries. ZIC is a registered trademark of SeQuant AB. Phenex is a trademark of Phenomenex, Inc. Disclaimer Px Advanced Technology Solutions Pvt. Ltd. is a part of Phenomenex. 0 Phenomenex, Inc. All rights reserved.