USP 3, 5, 10, 10- PREP

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1 ne of the World s Leading HPLC Columns Develop or Improve your HPLC Method Exceptionally rugged USP phases Easy method scalability with,,, -PREP and μm media Extensive batch traceability and reproducibility data supplied with every column If Luna analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, send in your comparative data within days and keep the Luna column for FREE. Material Characteristics Particle Shape/Size (μm) Pore Size (Å) Surface Area (m /g) Carbon Load % Calculated Bonded Phase Coverage (µmole/m ) End Capping ph Stability Packing Material Luna Silica() Spher.,,, -PREP, No Luna C Spher.,. 7.8 Yes. - 9.* Luna C8() Spher.,,, -PREP,.. Yes. - 9.* Luna C8() Spher..,,,, -PREP, 7.. Yes. - 9.* Luna Phenyl-Hexyl Spher.,,, -PREP, 7.. Yes. - 9.* Luna CN Spher.,, 7..8 Yes Luna NH Spher.,, 9..8 No. - Luna SCX Spher., Binding Capacity:. meq/g No Luna HILIC Spher.,.7. No Luna PFP() Spher.,.. Yes * ph range is. - under isocratic conditions. ph range is. - 9 under gradient conditions. USP Phases for Virtually Every Application Luna Bonded Phase Selectivity Chart USP Column Classification Phase Description Common Applications L L L7 L8 L9 L L L L C8().,,,, -PREP, µm C8 phase. Excellent efficiency, peak shape and resolution. Slightly lower carbon load than original Luna C8. Silica(),, µm Ultra-pure silica with high column bed stability enhanced by particle shape uniformity. C8(),,, -PREP, µm C8 phase for excellent efficiency, peak shape and resolution. Significantly improved performance over traditional C8 phases due to high surface coverage. NH,, µm Amino phase. Can be used in reversed or normal phase modes. Stable from ph. to. and under % aqueous conditions. High performance silica and bonding techniques produces a rugged, highly reproducible column. SCX, µm A Benzene Sulfonic Acid bonded phase is used to make this Strong Cation Exchange (SCX) column. ffers great peak shape and resolution. CN,, µm Cyano phase. Can be used as reversed- or normal-phase material. The use of Luna base silica results in overall phase reproducibility and performance. Phenyl-Hexyl,,, -PREP, µm A phenyl phase which employs a hexyl alkyl linker as opposed to the traditional propyl chain. ffers great stability as well as alternative selectivity. HILIC, µm HILIC phase that provides excellent selectivity for polar compounds; and improved MS sensitivity with low bleed. PFP(), µm A pentafluorophenyl phase that provides excellent selectivity for aromatic compounds from influence of flourine substitution on phenyl ring. Multiple retention mechanisms. rthogonal selectivity to traditional C8 phases. Acetaminophen, Aspirin, Caffeine, Albuterol, Amitriptyline Hydrochloride, Amoxicillin, Atenolol, Cephalexin, Cephradine capsules, Chloramphenicol, Cortisone Acetate, Dextromethorphan, Diphenhydramine, Pseudoephedrine, Dopamine, Estradiol, Guaifenesin, Ibuprofen, Sterile Imipenem, Imipramine, Lidocaine, Lorazepam, Minoxidil, Naproxen, Phenylephrine Hydrochloride, Phenylpropanolamine, Prednisone oral solution, Procainamide, Propoxyphene, Reserpine Alprazolam, Hydrocodone bitartrate, Hydrocortisone, Fat Soluble Vitamins, Phthalates, Fatty Acids, Lutein, Lycopene, Estradiol Doxepine, Doxylamine succinate, Fluoxetine, Glyburide, Ibuprofen ral Suspension, Propranolol, Levonorgestrel, Ethinyl estradiol, Melengestrol acetate, Glucosamine Simple sugars, Carboplatin, Lactulose concentrate, Levocarnitine tablets Cough and cold compounds, Raclopride, Sodium Acetate, Erythromycin Benzalkonium Chloride, Nortriptyline HCl Capsules, Prednisolone, Tetracaine, Quinapril tablets xacillin, Captopril, Chlorpheniramine, Pseudoephedrine, Methadone Hydrochloride ral Concentration Drug metabolites, Water soluble vitamins, Melamine, Cyanuric acid, Metanephrine, Normetanephrine Positional isomers, Geometric isomers, Taxanes, Aflatoxins HPLC Luna Try Gemini for even longer column lifetimes (see page 87). Phenomenex

2 Luna Silica A Backbone and Phase Designed for Long Column Lifetimes Luna columns excellent performance is not simply the result of ultra-pure metal-free silica (99.99 % purity). Meticulous care is given to the quality control of surface smoothness, pore structure and pore consistency to ensure particles of uniform structure and enhanced mechanical strength. Either bonded or unbonded, Luna silica produces highly advanced HPLC columns: Luna Silica() ph Stability:. 7. Particle Size: µm, µm, μm, μm-prep, and μm Phase: Unbonded silica Application: Polar compounds USP: L Low percentage of fines from damaged silica leading to lower backpressures and enhanced column performance and lifetimes High column bed stability enhanced by particle shape uniformity Incredible Silica Smoothness Luna silica is extremely smooth and spherical. For bonded phases, this provides a uniform bonding surface for consistent and even bonded phase coverage. The likelihood of silica particle shearing and breakage during bonding and packing is very low; thus, Luna columns have high efficiencies and long column lifetimes. Recommended for preparative and bulk packing into DAC systems, see page 6 for more information Compare Surface Smoothness Phenomenex Luna μm C8() LUNA HPLC Natural Products (Kava Kava) Column: Luna μm Silica() Part No.: F-7-E Mobile Phase: Hexane/Dioxane (8:) Flow Rate:. ml/min Detection: nm Sample:. Dihydrokawain. Yangonin. Kawain. Dihydromethysticin. Methysticin Waters Symmetry μm C8 min App ID 8 Agilent Technologies ZRBAX μm SB-C8 Phenomenex

3 Luna C8(), C8(), C Your Starting Point for Reversed Phase Methods The Luna column has found a place as one of the world s top reversed phase columns because it provides a measurable improvement over many HPLC columns for two important chromatographic properties: resolution and peak shape. The high efficiencies and bonded phase surface coverage provide for sharp peaks. The result: Free exposed silanols virtually eliminated by complete bonding and endcapping Sharp peak shape for good method sensitivity ph stable from. to. for over hours C8 Column Comparison** Luna μm C8() Luna C8() USP: L LC/MS Certified ph Stability:.-9.* Particle Size:. µm, μm, μm, μm, μm-prep, and μm Phase: C8, endcapped Application: Small molecules Strength: Wide ph stability provides longer column lifetime and greater method flexibility Luna C8() USP: L7 LC/MS Certified ph Stability:.-9.* Particle Size: μm, μm, μm, μm-prep, and μm Phase: C8, endcapped Application: Small molecules when less retention and greater speed is desired Strength: Lower silanol activity than C8() phase plus wide ph stability for longer column life and greater method flexibility mau 6 Salicylic acid Peak Area: Luna C LC/MS Certified ph Stability:.-9.* Particle Size: μm, μm Phase: C, endcapped Application: Small molecules when less retention and greater speed is desired Strength: Greater hydrolytic and ph stability compared to most C phases * ph range is. - under isocratic conditions. ph range is. - 9 under gradient conditions. App ID min mau Waters SunFire μm C8 HPLC Luna 6 Salicylic acid Peak Area: Pyridine Peak Asymmetry Comparison** Luna μm C8() min Aromatic Acids Columns: Luna μm C8() Å SunFire μm C8 Å Conditions for all columns: Mobile Phase: mm Phosphate buffer, ph. / Acetonitrile (7:) Flow Rate:. ml/min Detection: nm Sample:. p-hydrozybenzoic acid. Benzoic acid. Salicylic acid. p-toluic acid App ID 79 Sigma-Aldrich Biotechnology Supelcosil μm ABZ Sigma-Aldrich Biotechnology Supelcosil LC-8-BD Waters Spherisorb μm DS() Agilent Technologies ZRBAX μm SB-C8 G.L. Sciences Inertsil DS() Waters Symmetry μm C8 Comparison of 7 different μm reversed phase columns. This survey measures the degree of silanol activity on the surface of each silica. In this survey, Luna µm C8() material demonstrates the lowest silanol activity. ** The comparative data presented here may not be representative for all applications. Phenomenex

4 Luna C8(), C8(), C (cont d) Steroids Column: Luna μm C8() Part No.: F--E Mobile Phase:. % H P /Acetonitrile/Methanol (::) Flow Rate:.7 ml/min Detection: nm Sample:. Hydrocortisone. Corticosterone. -a-hydroxyprogesterone. Cortisone Acetate. -Ketoprogesterone App ID 8 Polynuclear Aromatic Hydrocarbons: EPA Method 8 Column: Luna μm C8 () Dimensions: x.6 mm Part No.: G--E Mobile Phase: A: Water B: Acetonitrile Gradient: A/B (:7) to % B in min Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Naphthalene. Acenaphthylene. Fluorene. Phenanthrene. Anthracene 6. Fluoranthene 7. Pyrene 8. Benz[a]anthracene 9. Chrysene. Benzo[e]pyrene. Benzo[b]fluoranthene. Benzo[k]fluoranthene. Benzo[a]pyrene. Dibenz[a,h]anthracene. Benzo[g,h,i]perylene 6. Indeno[,,-c,d]pyrene min Saw Palmetto Berry, p-bromophenacyl Esters Column: Luna μm C8() Part No.: F-8-E Mobile Phase: Acetonitrile/Water (87:) Flow Rate:. ml/min Temperature: C Detection: nm Sample:. Capric Acid. Lauric Acid. Linolenic Acid. Myristic Acid 7. Linoleic Acid 6. Palmitic Acid 7. leic Acid 8. Stearic Acid Acetaminophen, USP Method Column: Luna μm C8() Part No.: F--E Mobile Phase: Water/Methanol/Acetic Acid (69:8:) Flow Rate:. ml/min Detection: 7 nm Sample:. Acetaminophen. Caffeine. Benzoic Acid App ID 69 LUNA HPLC 6 8 App ID 7 min Pharmaceutical Preservatives Column: Luna μm C Part No.: F--E Mobile Phase: A:. % Acetic acid in water/acetonitrile (8:) B:. % Acetic acid in water/acetonitrile (:8) Gradient: A/B (:) to A/B (:) in min Flow Rate: ml/min Temperature: C Detection: nm Sample:. Propylparaben impurity. Benzyl alcohol. Phenol. Benzoic acid. Methylparaben 6. Benzaldehyde 7. Ethylparaben 8. Propylparaben Adenine Family Column: Luna μm C8() Part No.: F--E Mobile Phase: A: mm Ammonium acetate, ph. B: Acetonitrile Gradient: A/B (97:) to (6:) in min Flow Rate: ml/min Temperature: Ambient Detection: UV@ 6 nm Sample:. ATP. ADP. AMP. Adenine. Adenosine App ID App ID 76 App ID min 6 8 min 6 Phenomenex

5 Luna Phenyl-Hexyl Engineered for Stability Luna Phenyl-Hexyl columns provide separations not achievable on C8 or C8 columns; such as increased retention for polar, aromatic compounds as well as reversals in analyte elution order. Luna Phenyl-Hexyl columns are a reproducible, extremely stable phenyl phase. Most phenyl phases use a short propyl ( carbon) linker, which limits phase stability. The Phenyl-Hexyl bonded phase employs a phenyl ring with a hexyl (6 carbon) linker and is densely bonded to Luna silica surface, reducing bonded phase hydrolysis and increasing chemical stability. The result: Luna Phenyl-Hexyl USP: L LC/MS Certified ph Stability:.-9.* Particle Size: μm, μm, μm, μm-prep, and μm Phase: Phenyl with Hexyl (C6) linker, endcapped Application: Non-polar compounds Strength: Aromatic selectivity enhanced by higher hydrophobicity due to hexyl linker * ph range is. - under isocratic conditions. ph range is. - 9 under gradient conditions. Highly reproducible and stable phenyl phase Dual selectivity of both phenyl phase and a short alkyl phase (C or C8) Excellent retention of aromatic and polar, amine compounds Recommended for US EPA Method 8B for explosives analysis. to ph stability for over hours Chromatographic Comparisons of Phenyl Columns** Luna μm Phenyl-Hexyl Waters Spherisorb μm Phenyl 6 7 Peaks 7, 8 Some Absorption 8 App ID min App ID 8 HPLC Luna Agilent Technologies Zorbax μm SB-Phenyl Agilent Technologies Zorbax μm Phenyl Peaks, Poor Resolution Peaks,, 6 Poor Resolution App ID 8 Peaks 7, 8 Total Absorption App ID 8 Antibacterials Conditions for all columns: Mobile Phase: A: mm KH P, ph. B: Acetonitrile Gradient: Start A/B (8:) to A/B (7:) in min, go to A/B (:) in min Flow Rate:. ml/min Detection: nm Sample:. Carbadox. Thiamphenicol. Furazolidone. xolinic Acid. Sulfadimethoxine 6. Sulfaquinoxaline 7. Nalidixic Acid 8. Piromidic Acid **The comparative data presented here may not be representative for all applications. Phenomenex 7

6 Luna CN (cyano) Proven Reproducibility For carboxyl, carbonyl and amine containing compounds, Luna CN columns offer a unique polar selectivity in reversed phase and normal phase mode. Luna CN columns provide sharp peaks and great reproducibility run-to-run, column-to-column and batch-tobatch. The smooth silica allows for a more uniform bonding with improved resistance to bonded phase hydrolysis to produce one of the most stable CN phases. The result: Luna CN USP: L ph Stability:.-7. Particle Size: μm, μm, and μm Phase: Cyano, endcapped Application: Compounds with CH, C, NH, NHR, or NR Strength: Improved reproducibility for more consistent results run-to-run, column-to column, batch-to-batch LUNA HPLC Improved peak shapes ne of the most stable CN columns under reversed phase or normal phase conditions. ph stable from. to 7. Batch-to-Batch Reproducibility Column: Luna μm CN Mobile Phase: A: Hexane, B: Methylene chloride/methanol(8:), A/B (8:) Flow Rate:. ml/min Detection: nm Injection:. µl Temperature: Ambient Sample:. Hydrocortisone. Prednisone. Cortisone. Hydrocortisone Acetate Column-to-Column Reproducibility Column: Luna μm CN Mobile Phase: A: Hexane, B: Methylene chloride/methanol(8:), A/B (9:) Flow Rate:. ml/min Injection: µl Detection: nm Temperature: Ambient Sample:. Dimethyl phthalate. Diethyl phthalate. Dibutyl phthalate. Dioctyl phthalate App ID 6 App ID 66 CN Column Comparsion** Luna μm CN Waters Spherisorb μm CN Agilent Technologies Zorbax μm SB-CN App ID 76 App ID 7 App ID 7 Reversed Phase Conditions Column: Luna μm CN Mobile Phase: A: Acetonitrile, B: mm Potassium phosphate ph., A/B (:6) Flow Rate:. ml/min Detection: nm Sample: Tricyclic Antidepressants. Doxepin. Desipramine. Nortriptyline. Amitriptyline. Protriptyline min App ID 98 Normal Phase Conditions Conditions for all columns: Mobile Phase: A: Hexane, B: Methylene chloride/methanol (8:), A/B (99:)) Flow Rate:. ml/min Detection: nm Temperature: Ambient Sample: Phthalate Esters. Di-n-octyl phthalate. Bis (-Ethylhexyl) phthalate. Butylbenzyl phthalate. Di-n-butyl phthalate. Diethyl phthalate 6. Dimethyl phthalate **The comparative data presented here may not be representative for all applications. 8 Phenomenex

7 Luna NH (amino) Developed for Ruggedness Luna NH columns retain hydrogen-bonding compounds under three separation modes: Reversed Phase, Normal Phase, and Ion- Exchange. Luna NH columns provide reproducible retention and selectivity with improved column lifetime. Amino columns can be problematic as the bonded phase easily hydrolyses off the silica, shortening retention time over the life of the column. The bonded phase stability of Luna NH columns are illustrated by. to. ph stability and % aqueous mobile phase stability. The result: Increased bonded phase stability improves reproducibility and column lifetime Excellent retention of simple sugars, complex sugars, sugar alcohols and other hydrogen-bonding compounds under reversed phase, normal phase, ion-exchange conditions ph stable from. to. Stable in % aqueous mobile phases Simple Sugars Column: Luna μm NH Dimensions: x.6 mm Part No.: G-78-E Mobile Phase: Acetonitrile/Water (8:) Flow Rate: ml/min Temperature: C Detection: RI Sample:. Xylose. Fructose. Glucose. Sucrose. Maltose 6. Melezitose 7. Raffinose Luna NH USP: L8 ph Stability:.-. Particle Size: μm, μm, and μm Phase: Amino Application: Compounds with CH, C, NH, NHR, or NR Strength: Sugars by reversed phase, steroids by normal phase, oligonucleotides by ion exchange -8mer poly-dt ligonucleotide Column: Luna μm NH Part No.: F-78-E Mobile Phase: A: mm Sodium Phosphate, % Acetonitrile, ph 7. B: mm Sodium Phosphate, % Acetonitrile, M Sodium Chloride, ph 7. Gradient: A/B (7:) to A/B (:9) in minutes Flow Rate: ml/min Temperature: C Detection: 6 nm Sample: -8mer poly-dt oligonucleotide standard 6 7 App ID 8 min HPLC Luna Stability in % Aqueous Mobile Phase Steroids Column: Luna μm NH Dimensions: x.6 mm Part No.: G-78-E Mobile Phase: Hexane/Ethanol (8:) Flow Rate: ml/min Temperature: C Detection: nm Sample:. -Ketoprogesterone. -Hydroxyprogesterone. Cortisone Acetate. Prednisolone -Acetate. Cortisone 6. Prednisolone Column: Luna μm NH Dimensions: x.6 mm Part No.: G-78-E Mobile Phase: mm Potassium Phosphate Buffer ph.7 Flow Rate:. ml/min Detector: nm Temperature: Ambient Injection:. µl Conditions: Continuously flushed at. ml/min using % mm Potassium Phosphate Buffer ph.7 between injections App ID 99 App ID 68 Phenomenex 9

8 Luna SCX (strong cation exchange) Develop Robust Methods Luna SCX columns provide excellent resolution and peak shape of basic, cationic compounds. However, most SCX columns show poor peak shape and bad resolution causing many chromatographers to ignore this important phase for small molecule method development, until now. Luna SCX columns contain a benzene sulfonic acid ligand providing ion-exchange, reversed phase, and aromatic interactions. Such interactions make Luna SCX columns great as a first dimension for D LC applications as well as improved resolution for small molecules. The result: Luna SCX USP: L9 ph Stability:.-7. Particle Size: µm and µm Phase: Benzene Sulfonic Acid, Strong Cation Exchange Application: Amine and polyamine containing compounds Strength: Guaranteed to provide sharper peak shape and better resolution compared to traditional SCX columns LUNA HPLC Resolving power and sharp peak shape to separate complex cationic/basic and nitrogen containing compounds and μm columns and bulk media for analytical through preparative separations Benzene sulfonic acid ligand provides mixed-mode interaction improving separation for D peptide applications Childrens Tylenol Cold Syrup Column: Luna μm SCX Part No.: F-98-E Mobile Phase: mm KH P, ph./acetonitrile (:6) Injection Volume: µl Flow Rate:. ml/min Detection: nm Sample Prep: Dissolve part Childrens Tylenol Cold in parts Methanol Sample:. Acetaminophen. Pseudoephedrine. Dextromethorphan. Chlorpheniramine Peptides Column: Luna μm SCX Part No.: F-98-E Mobile Phase: A: mm Potassium Phosphate, % Acetonitrile, ph. B: mm Potassium Phosphate, % Acetonitrile, mm Potassium Chloride, ph. Gradient: A/B (9:) to A/B (:9) in minutes Flow Rate: ml/min Temperature: C Detection: nm Injection Volume: µl ( µg on column) Sample: Peptide Mixture - Substance P. Fragment - (+). Fragment - (+). Fragment - (+). Fragment -9 (+). Intact (+) 6. (ARG-PHE-TRP-LEU) (+) 6 App ID 76 min 6 8 min App ID Tryptic Digest of Bovine Cytochrome c Column: Luna μm SCX Part No.: F-98-E Mobile Phase: A: mm Potassium Phosphate, ph. / % Acetonitrile B: mm Potassium Phosphate, ph. / % Acetonitrile / mm Potassium Chloride Gradient: % A to % B in minutes Flow Rate: ml/min Temperature: C Detection: nm Injection Volume: µl ( µg on column) Sample: Bovine Cytochrome c trypsin digest SCX Method Development and ph: The standard operating ph range for Luna SCX columns is. to 7.. Most SCX methods are typically run between ph. and. for optimal performance. This ensures that nitrogen-containing analytes, especially those with adjacent conjugated system are protonated. Running in highly acidic (ph <.) or basic (ph > 7.) mobile phases may cause this phase to undergo degradation, as is common for all silica-based SCX phases. App ID 767 min Phenomenex

9 H Luna Luna HILIC Increase MS Sensitivity and Retention for Polar Compounds Luna HILIC columns retain a water-enriched layer on the surface of the silica. This water layer facilitates the transfer of polar compounds onto the stationary phase for increased retention. Hydrophilic Interaction Liquid Chromatography (HILIC) is a separation mode where the partitioning of polar solutes from the high concentration, water-miscible, organic mobile phase into the hydrophilic surface environment creates separations. Polar solutes exhibit increased retention and elute in the order of increasing hydrophilicity. Finally, reproducible, robust HILIC separations! Made for retention of polar compounds Increase mass spectrometry sensitivity Increase laboratory throughput and productivity H H H H H - H H H H H Vitamin Mix on Luna HILIC Vitamins provide an excellent platform to demonstrate the benefits of HILIC. The effect of increased polar compound retention can be easily seen in this application. to min Column: Luna μm HILIC Part No.: F--E Mobile Phase: A: Acetonitrile B: Water C: mm Ammonium Acetate, ph.8 Gradient: A/B/C (9::) for. min to A/B/C (::) in 7. min, hold for. min. A/B/C (9::) for 7. min Flow Rate:. ml/min Detection: 6 nm Sample:. p-aminobenzoic Acid pk a.7, H + pk a.7 logp.8. Nicotinamide H + pk a. logp.7. Riboflavin pk a. logp.6. Nicotinic Acid pk a.7, H+pK a. logp.6. Pyridoxine H + pk a.6, pk a 8.6 logp Thiamine H + pk a. logp.6 7. Ascorbic Acid pk a.,. logp.8 8. Cyanocobalamin pk a.9 logp.9 9. Folic Acid pk a.7,., 8.9 logp. Ganciclovir & Acyclovir on Luna HILIC HILIC uses a solvent system that is the reverse of what is common in reversed phase, thus peak elution order is reversed on the Luna HILIC column. Polar / Hydrophilic compounds that are particularly difficult to retain, even on polar-embedded reversed phase columns, will enjoy maximum retention on Luna HILIC columns. 9 App ID 6 HPLC Luna Luna HILIC Polar Embedded C8 More Polar Compound = More Retention! Column: Luna µm HILIC Dimensions: x. mm Part No.: D-9-B Mobile Phase: Acetonitrile/ mm Ammonium Formate, ph. (9:) Flow Rate:. ml/min Detection: Mass Spectrometer (MS) Sample:. Acyclovir. Ganciclovir.e.e.e.e Column: Synergi µm Fusion-RP Dimensions: x. mm Part No.: D--B Mobile Phase: Acetonitrile/ mm Ammonium Formate, ph. (:97) Flow Rate:. ml/min Detection: 6 nm Sample:. Acyclovir. Ganciclovir min App ID min App ID 6 Phenomenex

10 Luna HILIC (cont d) Improved MS Sensitivity The increased retention in HILIC allows elution of the analytes outside the suppression region and thus increases detector sensitivity. In addition, the Luna HILIC column also resolves the compounds with the reverse order of that seen in reversed phase LC. Nicotine and Metabolites. Cotinine Peak Area,7 Nicotine Peak Area 8,9 Increase in sensitivity Nornicotine Peak Area 69,. ng on column Luna HILIC min App ID 6.e.8e.6e.e.e.e.8e.6e.e.e.e.8e.6e.e.e.e Cotinine Peak Area 97, Nornicotine Peak Area,8 Nicotine Peak Area,89 Elutes in ion suppression zone!. ng on column min App ID 6 C8 Column: Luna µm HILIC Dimensions: x. mm Part No.: D--B Mobile Phase: Acetonitrile/ mm Ammonium Formate, ph. (9:) Flow Rate:. ml/min Detection: Mass Spectrometer (MS) Sample:. Cotinine (MRM: 77/8). Nicotine (MRM: 6/8). Nornicotine (MRM: 9/8) Column: Gemini µm C8 Dimensions: x. mm Part No.: D--B Mobile Phase: A: Water with. % v/v Formic Acid B: Acetonitrile with. % v/v Formic Acid Gradient: A/B (97:) hold min, to (7:) in min, to (97:) in. min, hold for. min Flow Rate:. ml/min Detection: Mass Spectrometer (MS) Sample:. Nornicotine (MRM: 9./8.). Nicotine (MRM: 6./.9). Cotinine (MRM: 77./8.) LUNA HPLC Unique HILIC Selectivity Not all HILIC columns are alike, Luna HILIC columns deliver on the exacting standards you have come to trust from the Luna product line. HILIC Column Comparison** Luna μm HILIC Waters Atlantis μm HILIC to 6 Baseline resolution of all peaks Peak 6 (Thiamine-no elution) App ID 67 to App ID min 6 8 min Conditions for all columns: Column: As noted Mobile Phase: Acetonitrile/ mm Ammonium Formate, ph. (9:) Flow Rate:. ml/min Detection: 6 nm Sample:. PABA. Nicotinamide. Riboflavin. Nicotinic Acid. Pyridoxine 6. Thiamine to 6 8 SeQuant μm ZIC -HILIC Peak 6 (Thiamine-no elution) min App ID 6 ** The comparative data presented here may not be representative for all applications. Phenomenex

11 Luna PFP() Powerful Selectivity for Reversed Phase Methods Luna PFP() columns provide remarkable selectivity for highly polar compounds, complex natural products, isomers, and other closely related compounds. This is achieved by using a pentafluorophenyl with a propyl linkage which provides multiple retention mechanisms different to other reversed phase media. Achieve excellent selectivity using four mechanisms of solute/stationary phase interactions Extremely discerning for halogenated, aromatic and conjugated compounds Provides orthogonal selectivity even using traditional reversed phase mobile phase systems Luna PFP() Selectivity is achieved through of the mechanisms of interaction Hydrogen Bonding Dipole-Dipole Interactions Aromatic and π-π Interactions Hydrophobic A typical alkyl phase (C8, C8) achieves selectivity through only mechanism of interaction. Positional Isomers of Halogenated Phenols Halogens can radically increase the polarity of a compound, thus decreasing typical retention characteristics. Luna PFP() columns retain, discriminate, and separate halogens easily. Luna μm PFP() Luna μm C8() 9 9 & Coelution! Baseline separation! App ID App ID 697 HPLC Luna 6 8 min 6 8 min Columns: Luna µm PFP() Luna µm C8() Part Nos.: F-7-E F--E Conditions for all columns: Mobile Phase: A:. % Formic acid in Water B:. % Formic acid in Acetonitrile Gradient: A/B (6:) to (:) in min Flow Rate: ml/min Temperature: C Detection: nm Sample:.,-Dimethylphenol.,-Dimethylphenol.,6-Dimethylphenol.,-Dimethylphenol.,-Dimethylphenol 6.,-Dichlorophenol 7.,6-Dichlorophenol 8.,-Dichlorophenol 9.,-Dichlorophenol.,-Dibromophenol Phenomenex

12 rdering Information. μm High Speed Technology (HST) Columns (mm) Phase x. x. x. x. x. Luna. µm C8()-HST A-6-B B-6-B D-6-B B-6-Y D-6-Y If Luna analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase, and dimensions, send in your comparative data within days and keep the Luna column for FREE. For information about HST Columns, contact your Phenomenex technical consultant or local distributor. μm and μm Capillary Columns (mm) Guard Columns (mm) Phases x. x. x. x. x. x. x. x. μm C8() B-8-AC F-8-AC B-8-AF F-8-AF M-8-AC M-8-AF μm C8() B--AC F--AC G--AC B--AF F--AF G--AF M--AC M--AF μm C8() F-9-AC G-9-AC F-9-AF M-9-AC M-9-AF μm C8() B--AC F--AC G--AC B--AF F--AF G--AF M--AC M--AF μm Phenyl-Hexyl B-7-AC B-7-AF For Fused Silica Capillary Adapter, see p. 67 MercuryMS LC/MS Cartridges (mm) Columns (mm) μm Phase x. x. x. x. x. x. Luna C8() N--B-CE N--D-CE M--B-CE M--D-CE M--B M--D Luna C8() N-8-B-CE N-8-D-CE M-8-B-CE M-8-D-CE M-8-B M-8-D μm Phase x. x. x. Luna C8() N--B-CE M--B-CE M--D-CE Luna C8() N-9-B-CE M-9-B-CE M-9-D-CE LUNA HPLC MercuryMS Cartridge Holders Direct-Connect Holder Standard Holder rdering Information Direct-Connect Cartridge Holders Part No. Description Price CH-787 mm direct-connect holder CH-788 mm direct-connect holder Standard Cartridge Holders Part No. Description Price CH-86 mm standard holder CH-8 mm standard holder MercuryMS Screening Kits These convenient screening kits allow quick, easy and economical evaluation of multiple phases. Each kit contains one cartridge of each available phase and a standard holder. Available in either μm or μm Luna with. μm Synergi. rdering Information Screening Kits x. mm Kit x. mm Kit Kit A includes ea of: KH-7 KH-7 Luna μm C8() Cartridge Luna μm C8() Cartridge Synergi. μm Max-RP Cartridge Synergi. µm Hydro-RP Cartridge mm Standard Cartridge Holder Kit B includes ea of: KH-7 KH-76 Luna μm C8() Cartridge Luna μm C8() Cartridge Synergi. μm Max-RP Cartridge Synergi. μm Hydro-RP Cartridge mm Standard Cartridge Holder rdering Information μm Microbore and Minibore Columns (mm) *SecurityGuard Analytical Cartridges require holder, Part No.: KJ-8 securityguard Cartridges (mm) Phases x. x. x. x. x. x. x.* /pk Silica() A-6-B B-6-B D-6-B F-6-B AJ-7 C8() B-8-A F-8-A A-8-B B-8-B D-8-B F-8-B AJ-89 C8() B--A F--A A--B B--B D--B F--B AJ-86 CN A--B B--B D--B F--B AJ- Phenyl-Hexyl B-6-A A-6-B B-6-B D-6-B F-6-B AJ- NH F-77-A A-77-B B-77-B D-77-B F-77-B AJ- HILIC B-9-B D-9-B F-9-B AJ-88 PFP() A-7-B B-7-B D-7-B F-7-B AJ-86 for ID:.-. mm Phenomenex

13 rdering Information SecurityGuard μm Narrow Bore and Analytical Columns (mm) Cartridges (mm) Phases x. x. x. x.6 x.6 7 x.6 x.6 x.6 x.* x.* /pk /pk Silica() F-6-Y B-6-E D-6-E F-6-E AJ-7 AJ-8 C8() A-8-Y B-8-Y F-8-Y A-8-E B-8-E C-8-E D-8-E F-8-E AJ-89 AJ-9 C8() A--Y B--Y F--Y A--E B--E C--E D--E F--E AJ-86 AJ-87 CN B--Y F--Y A--E B--E C--E D--E F--E AJ- AJ- Phenyl-Hexyl B-6-Y F-6-Y A-6-E B-6-E C-6-E D-6-E F-6-E AJ- AJ- NH B-77-Y F-77-Y B-77-E D-77-E F-77-E AJ- AJ- HILIC B-9-Y F-9-Y D-9-E F-9-E AJ-88 AJ-89 PFP() B-7-Y F-7-Y B-7-E D-7-E F-7-E AJ-86 AJ-87 for ID:.-. mm.-8. mm SecurityGuard μm Microbore and Minibore Columns (mm) Cartridges (mm) Phases x. x. x. x. x. x. x. x.* /pk Silica() B-7-B F-7-B G-7-B AJ-7 C A--B B--B F--B AJ-9 C8() B-9-A F-9-A A-9-B B-9-B F-9-B G-9-B AJ-89 C8() B--A F--A G--A A--B B--B F--B G--B AJ-86 CN A--B B--B F--B G--B AJ- Phenyl-Hexyl B-7-A G-7-A A-7-B B-7-B F-7-B G-7-B AJ- NH F-78-A A-78-B B-78-B F-78-B G-78-B AJ- PFP() A-8-B B-8-B F-8-B AJ-86 for ID:.-. mm μm Narrow Bore and Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases x. x. x. x. x.6 x.6 7 x.6 x.* x.* /pk /pk Silica() B-7-E AJ-7 AJ-8 C F--Y B--E AJ-9 AJ-9 C8() B-9-Y F-9-Y G-9-Y A-9-E B-9-E C-9-E AJ-89 AJ-9 C8() A--Y B--Y F--Y G--Y A--E B--E C--E AJ-86 AJ-87 CN B--Y F--Y G--Y A--E B--E C--E AJ- AJ- Phenyl-Hexyl A-7-Y B-7-Y F-7-Y G-7-Y A-7-E B-7-E C-7-E AJ- AJ- NH B-78-Y F-78-Y G-78-Y A-78-E B-78-E C-78-E AJ- AJ- SCX B-98-E AJ-7 AJ-8 HILIC F--Y AJ-88 AJ-89 PFP() B-8-Y F-8-Y B-8-E AJ-86 AJ-87 for ID:.-. mm.-8. mm HPLC Luna μm Analytical and Semi-Prep Columns (mm) SecurityGuard Cartridges (mm) Phases x.6 x.6 x.6 x x x.* x /pk /pk Silica() D-7-E F-7-E G-7-E G-7-N AJ-8 AJ-7 C D--E F--E G--E AJ-9 AJ-77 C8() D-9-E F-9-E G-9-E G-9-N AJ-9 AJ-7 C8() D--E F--E G--E G--N G--AK AJ-87 AJ-7 CN D--E F--E G--E G--N AJ- AJ-7 Phenyl-Hexyl D-7-E F-7-E G-7-E G-7-N AJ- AJ-7 NH D-78-E F-78-E G-78-E G-78-N AJ- AJ-76 SCX D-98-E F-98-E G-98-E G-98-N AJ-8 AJ-769 HILIC D--E F--E G--E G--N AJ-89 AJ-89 PFP() D-8-E F-8-E G-8-E G-8-N AJ-87 AJ-876 for ID:.-8. mm 9-6 mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ-8 SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ-7 Phenomenex

14 If Luna analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase, and dimensions, send in your comparative data within days and keep the Luna column for FREE. rdering Information Axia Packed Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases x. x. x. x. x x x x.** x u $.8,6 $., $.69,77 $.7, $ Inquire., $ Inquire.6,9 su auf Inquire richiesta Anfrage /ea /ea μm Silica() B-7-P-AX D-7-P-AX F-7-P-AX G-7-P-AX B-7-U-AX D-7-U-AX G-7-U-AX AJ-79 AJ-8 C F--P-AX C8() B-9-P-AX D-9-P-AX F-9-P-AX B-9-U-AX D-9-U-AX AJ-78 AJ-8 C8() B--P-AX D--P-AX F--P-AX G--P-AX B--U-AX D--U-AX G--U-AX AJ-789 AJ-8 CN B--P-AX F--P-AX G--P-AX D--U-AX AJ-8 AJ-8 Phenyl-Hexyl B-7-P-AX D-7-P-AX F-7-P-AX B-7-U-AX D-7-U-AX AJ-78 AJ-8 NH D-78-P-AX F-78-P-AX G-78-P-AX AJ-86 AJ-89 PFP() B-8-P-AX D-8-P-AX F-8-P-AX G-8-P-AX B-8-U-AX D-8-U-AX G-8-U-AX AJ-877 AJ-878 HILIC B--P-AX D--P-AX F--P-AX G--P-AX G--U-AX for ID: 8-9 mm -9 mm Axia Packed Preparative Columns (mm) (continued) SecurityGuard Cartridges (mm) Phases x. x. x. x x x x x.** x u su auf Inquire richiesta Anfrage su auf Inquire richiesta Anfrage su auf Inquire richiesta Anfrage /ea /ea μm Silica() G-9-P-AX G-9-U-AX AJ-79 AJ-8 C D-9-P-AX G-9-P-AX G-9-V-AX C8() B--P-AX G--P-AX B--U-AX G--V-AX AJ-78 AJ-8 C8() B--P-AX D--P-AX G--P-AX B--U-AX G--U-AX B--V-AX G--V-AX AJ-789 AJ-8 CN G--P-AX AJ-8 AJ-8 Phenyl-Hexyl G-8-P-AX G-8-U-AX AJ-78 AJ-8 NH G-79-P-AX AJ-86 AJ-89 for ID: 8-9 mm -9 mm LUNA HPLC μm Analytical and Semi-Prep Columns (mm) SecurityGuard Cartridges (mm) Phases x.6 x x.* x / pk / pk Silica() G-9-E G-9-N AJ-8 AJ-7 C G-9-E G-9-N AJ-9 AJ-77 C8() G--E G--N AJ-9 AJ-7 C8() G--E G--N AJ-87 AJ-7 CN G--E G--N AJ- AJ-7 Phenyl-Hexyl G-8-E G-8-N AJ- AJ-7 NH G-79-E G-79-N AJ- AJ-76 SCX G--E G--N AJ-8 AJ-769 for ID:.-8. mm 9-6 mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ-8 SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ-7 **PREP SecurityGuard Cartridges require holder, Part No.: AJ-8 PREP SecurityGuard Cartridges require holder, Part No.: AJ-877 μm Pilot Scale Columns (mm) Phases x.6 Silica() C8() C8() Phenyl-Hexyl G-7-E G-7-E G-7-E G-86-E See our latest developments in High-throughout Purifications starting on page 6 For more dimensions and phases of Axia packed preparative columns, see pp. - For SecurityGuard Cartridge Holders and Cartridges, see p. For Bulk Media, see p. 6 Method development column kits and method validation column kits are available. Contact Phenomenex for details. Improve analyte sensitivity and reduce baseline noise with Strata SPE tubes and well plates, see p. for more information 6 Phenomenex