35 % OFF HPLC. The Ultimate Guide to

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1 The Ultimate Guide to HPLC Reversed Phase Selectivity 5 % FF Your Perfect Column Expires: 0 November 0 Promo Code: RPNV *ffer applies to products listed in this brochure and for columns with ID less than 0 mm. Phenomenex India Laxmi Cyber City, Ground Floor, B Block, Survey no: 0 Kondapur, Hyderabad , India Tel: Fax: indiainfo@phenomenex.com Web:

2 phenomenex.com We want to support you and your work in any way we can! Please visit for free technical resources, easy-to-find products, and useful tools to make your work easier! Take a journey to and find: Thousands of application notes with compound structures to search and download A virtual stockroom you can create for your lab Technical demonstrations and how-to videos Tools that make selecting the right product easier than ever And so much more! 0 Phenomenex, Inc. All rights reserved.

3 How do you choose a column? Do you reach into a drawer of mystery columns, look to your favorite C8 phase, or borrow one from a colleague? How about choosing the right column based on the right solid support, the right phase selectivity, and the right material characteristics for your method. We have developed this selectivity guide for you to quickly find the perfect column for your method. Follow this Step-by-step Selectivity Guide Contained within the following pages is an easy overview of the reversed phase HPLC options available to you. At a glance, you ll be able to quickly understand the differences between the columns available and select the right solution for your specific method and goals. STEP Select the Right Solid Support H Si H Si STEP Determine the Right Selectivity STEP Choose the Right Column...9- Recommendations for Special Cases (e.g. 00 % aqueous mobile phase)...0- Gemini extended lifetime in extreme ph conditions...- Synergi unique phases for challenging separations...-5 Luna variety of phases, particle sizes, and dimensions nyx high-throughput analysis... 8 Extend HPLC Column Performance... 9 rdering Information Technical Support Services... 6

4 Select the Right Solid Support Phenomenex offers a full range of solid supports including fully porous traditional and organo silica and monolithic silica rod. The morphology of the solid support has a significant impact on the resulting material characteristics and column performance. Fully Porous Traditional Silica Fully porous silica particles have higher surface area and provide excellent mechanical strength across a wide range of particle sizes and column dimensions. Well suited for: Seamless scale-up from analytical to a preparative or process application Direct column equivalent to those used in established Pharmacopeia methods Scalability Capillary Minibore Narrow Bore Analytical Semi-Prep Preparative Bulk Media Particle Sizes.5 µm µm µm 5 µm 0 µm 5 µm Fully Porous rgano-silica rganic groups are grafted into the layers of the silica particle making it more resistant to silica dissolution at higher phs. Well suited for: Extended column lifetime for methods run at ph extremes Premier bulk material product allowing for caustic washes for repeat use Scalability Capillary Minibore Narrow Bore Analytical Semi-Prep Preparative Bulk Media Particle Sizes.5 µm µm µm 5 µm 0 µm 5 µm Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

5 Monolithic Silica Rod Silica monolithic columns contain macropores and mesopores which allow for exceptionally low backpressure at high mobile phase linear velocities or when working with samples or mobile phases with increased viscosity. Well suited for: The direct injection of dirty samples such as plasma or foodstuff extracts Dramatically reducing run times by increasing flow rate from ml/min to 9 ml/min Scalability Capillary Minibore Narrow Bore Analytical Semi-Prep Preparative Bulk Media Particle Sizes N/A Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web: 5

6 The Importance of Selectivity Selectivity (α) has the greatest impact on changing resolution (R), as compared to efficiency (N) and retention (k), and the easiest way to change your chromatographic results is to change your column phase. Phenomenex develops a wide breadth of phase chemistries for easier and faster method development and optimization. The Impact of Selectivity on Resolution Selectivity is the most important parameter for increasing resolution. Use the selectivity profiles (pp. -8) to the find the right phase for your sample. Resolution (R) R = ( Efficiency )( Retention )( Selectivity ) Ν α - + α α Ν α Ν Change Your Selectivity, Dramatically Change Your Results mau 0 Gemini -NX µm C8 9 % Increase in Resolution mau Gemini-NX 5 µm C8 0 Å Resolution = 0.9 min Conditions same for all columns: Columns: as noted Dimensions: 50 x.6 mm Mobile Phase: A: 0 mm Potassium phosphate, ph.5 B: Acetonitrile Gradient: A/B (75:5) to (5:85) in 5 minutes Flow Rate:.5 ml/min Temperature: Ambient Detection: UV (ambient) Sample:. Impurity A. xymetazoline App ID 968 ption : Increase Efficiency (5 µm to µm particle) ption : Change Selectivity (C8 to ether-linked phenyl) mau Resolution =.00 min Synergi µm Polar-RP min 50 % Increase in Resolution! Resolution =. App ID 968 App ID Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

7 Characterizing Selectivity Phenomenex analyzes six different parameters to characterize the selectivity of our HPLC columns. Though hydrophobicity is a dominant retention mechanism in reversed phase chromatography, selectivity is strongly influenced by the other parameters described below. 6 Parameters Influencing Selectivity These interactions occur with all analytes. They are always present and are dominant for neutral compounds. Steric Influences A measurement of the accessibility of solutes to the stationary phase. Structural differences between compounds can lead to different retention characteristics due to shape selectivity. B H Hydrogen Bond (H-bond) Donating Capacity This interaction can be attributed to an exposed silanol or an intentionally added polar functional group. Phenomenex employs the latter technique to create phases that have the ability to hydrogen bond with proton accepting groups like weak bases (amines and amides). H X Hydrogen Bond (H-bond) Accepting Capacity Like the hydrogen bond donating capacity parameter, Phenomenex engineers phases that have the ability to hydrogen bond and interact with proton donating acidic groups such as carboxylic acids or alcohols. B + At neutral ph, residual silanols on the silica surface will be largely ionized, increasing the cation exchange component of selectivity. Cation Selectivity at ph.8 At low ph, most residual silanols are neutral and the cation exchange component will be reduced. Turn to the next page to see how we ve developed unique selectivity profiles for our columns based on these parameters. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web: 7

8 Column Selectivity Profiles Measurements of the parameters described on page 7 were independently derived at the BASi Northwest Laboratory to develop unique selectivity profiles for each of our Gemini, Luna, Synergi, and nyx phases. These profiles were developed so that chromatographers would have a dependable approach for comparing the Phenomenex phases and identifying which phase(s) would provide the best selectivity for their analytes. Example Luna C8() ➊ ➋ Steric Interaction ➌ Hydrogen Bond Donating Capacity ➍ Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 ➎ Do you need? ➊ Maximum retention hydrophobicity values indicate strong retention characteristics for any carbon containing analyte est hydrophobicity phases: Synergi Hydro-RP, Luna C8(), and Synergi Max-RP ➋ Separation of isobaric/isomeric compounds Stationary phases with high steric interaction values are best suited for the analysis of isomers and/or isobaric compounds est steric interaction phases: Luna C8(), Gemini C6-Phenyl, and Synergi Max-RP Important! Measurements illustrated here are not absolute, but a relative measurement to other Phenomenex columns. In this display, the individual measurements cannot be compared to each other. ➌ Retention and/or separation of polar, nitrogen containing compounds Bonded phases with high hydrogen bond donating capacity may help increase retention and selectivity of bases such as amines and amides est H-bond donating capacity phases: Synergi Hydro-RP, Gemini C8, and Synergi Max-RP ➍ Retention and/or separation of polar compounds containing alcohol or carboxylic acid groups Bonded phases with high hydrogen bond accepting capacity will preferentially interact with oxygen containing compounds, such as phenols and carboxylic acids, and may offer increased retention and selectivity est H-bond accepting capacity phases: Gemini C8, Synergi Fusion-RP, and Luna C8() ➎ Improved peak shape or better retention for charged bases Bonded phases with high cation selectivity values at low or neutral ph will show higher retention for ionized bases, but may show broad peaks Columns that have low cation selectivity values at low or neutral ph will have less interaction and less retention for charged bases, but may have very good peak shape est cation selectivity phases: Synergi Polar-RP and Luna PFP() est cation selectivity phases: Gemini C6-Phenyl, Luna C8(), and Luna C8() 8 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

9 Choose the Right Column Both the solid support and the bonded phase should be taken into consideration when selecting the most appropriate reversed phase HPLC column. Though the chart below depicts several similar bonded ligand types, no two columns are the same. View the selectivity profiles of each phase, pages 8, to see how each column can provide you with a truly different selectivity. Variety of Selectivities and Solid Supports for RP-HPLC Methods Fully Porous Silica Fully Porous Silica Fully Porous rgano-silica Monolithic Silica Rod C8 with nonpolar endcapping Luna C8() Gemini C8 Gemini-NX C8 nyx C8 C8 with polar embedded groups C8 with polar endcapping C with nonpolar endcapping Synergi Fusion-RP Synergi Hydro-RP Synergi Max-RP C8 with nonpolar endcapping Luna C8() nyx C8 C5 with nonpolar endcapping Luna C5 Phenyl with ether linkage and polar endcapping Synergi Polar-RP Phenyl with nonpolar endcapping Luna Phenyl-Hexyl Gemini C6-Phenyl PFP with nonpolar endcapping Luna PFP() CN with nonpolar endcapping Luna CN Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web: 9

10 } } Column Recommendations for Special Cases Based on experience and customer feedback, we ve found that particular solid support/selectivity combinations work very well for specific application types. We recommend the following columns as starting points for those applications outlined below. The selectivity profiles located in the specific product pages can also be utilized to identify a suitable column based on analyte characteristics. ph Recommended Columns: Gemini (all phases) Polar bases are neutral at ph 0.5, eliminating secondary ionic interactions min APP ID 66 Polar Bases (Beta Blockers) at ph Column: Gemini-NX 5 µm C8 Dimensions: 50 x.6 mm Mobile Phase: A: 0 mm Ammonium Bicarbonate ph 0.5 B: Acetonitrile Gradient: A/B (85:5) to (70:0) in 5 min to (50:50) in 5 min, Hold for 5 min Flow Rate:.5 ml/min Temperature: Ambient Detection: 0 nm Sample:. Bisoprolol Contaminant. Sotalol. Atenolol. Labetolol (Diastereoisomeric Pair) 5. Nadolol (Diastereoisomeric Pair) 6. Pindolol 7. Metoprolol 8. Bisoprolol 9. Propranolol 0. Alprenalol Direct Injection of Dirty Matrices ( Dilute-and-Shoot ) Recommended Columns: nyx (all phases) Intens. x 0 5 Sample Human urine sample diluted : Preparation: with water. 50 µl injected. Column: nyx Monolithic C8 Dimensions: 00 x.6 mm Part No.: CH0-76 Mobile Phase: A: 0. % Formic acid in Water B: 0. % Formic acid in Acetonitrile Gradient: 5-90 % B in.0 min Flow Rate:.0 ml/min Temperature: 0 C Detection: LC/MS ESI+ Injection:. Nordiazepam (Diazepam metabolite) (m/z = 7). Diazepam (m/z = 85) Don t Worry dilute-and-shoot applications can be done without damaging the column 0 0 min App ID Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

11 Aqueous Recommended Columns: Synergi Hydro-RP, Synergi Polar-RP, Synergi Fusion-RP Catecholamines Greater retention of polar compounds under 00 % aqueous conditions App ID 7 Column: Synergi µm Hydro-RP Dimensions: 50 x.6 mm Part No.: 00F-75-E0 Mobile Phase: 0 mm Potassium phosphate, ph.5 Flow Rate:.0 ml/min Temperature: C Detection: 0 nm Sample:. Norepinephrine. Epinephrine. 6-Hydroxydopamine. Normetanephrine 5. Dopamine 6. L-DPA 7. Epinine min Aqueous Stability Retention Time (min) ml of Buffer Aqueous stable for over 7,000 ml of buffer Norepinephrine Epinephrine Normetanephrine Dopamine L-DPA Column: Synergi µm Hydro-RP Dimensions: 50 x.6 mm Part No.: 00F-75-E0 Mobile Phase: 0 mm Potassium phosphate, ph.5 Flow Rate:.0 ml/min Temperature: 5 C Detection: 0 nm Injection: 5 µl Sample:. Norepinephrine (0.8 mg/ml). Epinephrine (0.5 mg/ml). Normetanephrine (0.6 mg/ml). Dopamine (0. mg/ml) 5. L-DPA (0. mg/ml) Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

12 The Standard for ph Method Development Rugged HPLC columns that offer extended lifetime under extreme ph conditions (ph -) and excellent stability for reproducible, high efficiency separations. Phases Ligand Description Selectivity Profile USP: L Gemini-NX C8 New generation of organo-silane material incorporates ethylene bridges to provide ph stability from - and 5x the durability of earlier hybrids. The homogenous surface offers some steric selectivity. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 USP: L Gemini C8 This is a high loading, organo-silane particle column with ph stability -. The patented procedure creates a surface that is a strong hydrogen donor and accepter. It is ideal for acids and bases. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 USP: L Gemini C6-Phenyl This is a very inert phase for great peak shapes of ionized compounds The planar phenyl rings offer moderate hydrophobic retention and high steric selectivity for structural isomer selectivity. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Material Characteristics Particle Shape/ Pore Size Surface Area Carbon Load Packing Material Size (µm) (Å) (m /g) % Endcapping ph Range Gemini C8 Spherical, 5, Gemini C6-Phenyl Spherical, Gemini-NX C8 Spherical, 5, Important! Measurements illustrated here are not absolute, but a relative measurement to other Phenomenex columns. In this display, the individual measurements cannot be compared to each other. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

13 Rugged, Dependable Columns Under the Most Extreme Conditions The harshest conditions for HPLC columns can be found in environments where columns are subjected to constant changes in ph, buffers, and temperature. A Gemini column s ability to hold up under these challenging conditions is a testament to the long column lifetimes and reproducible performance you can expect. Extend Column Lifetime Under Harsh ph Conditions Retention in Minutes 6 5 Step x ph (0.5) Gradient Flush Step ph (0.5) Testing Step x Neutral Flush Procedure Step Neutral ph Testing Step 5 x ph (.0) Gradient Flush Step 6 Neutral ph Flush Repeats Repeats for 0 Cycles For full procedure and parameters, visit Acenaphthene Toluene Sequence Benzene Acetophenone Column: Gemini-NX 5 µm C8 Dimensions: 50 x.6 mm Part No.: 00F-5-E0 Retention times maintained after exposure to 0,000 column volumes of high and low ph buffers Complementary Selectivities at Extended phs mau mau Gemini C8 can provide a different selectivity to your current C8 column for better resolution Gemini 5 µm C8 App ID 5606 min Traditional 5 µm C App ID 5608 min mau mau Greater retention and resolution can be achieved with Gemini C6-Phenyl over your current phenyl column Gemini 5 µm C6-Phenyl App ID 5885 Traditional 5 µm Phenyl min App ID 5886 min Dimensions: 50 x.6 mm Mobile Phase: 0 mm Phosphate buffer, ph.5 / Acetonitrile (50:50) Flow Rate: ml/min Temperature: Ambient Detection: 0 nm (ambient) Sample:. Ethyl paraben. Naproxen. Diflunisal. Indomethacin 5. Ibuprofen Dimensions: 50 x.6 mm Mobile Phase: 0 mm Ammonium bicarbonate, ph 0.5 / Acetonitrile / Methanol (0:5:5) Flow Rate: ml/min Temperature: Ambient Detection: 5 nm (ambient) Sample:. Imipramine. Nortriptyline. Amitriptyline. Clomipramine Dimensions and chromatographic conditions are the same for all columns unless otherwise noted. Comparative separations may not be representative of all applications. See page 0 for ordering information. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

14 Full Range Selectivity Four unique phases developed to provide a different selectivity for successful separations of the most complex mixtures and challenging analytes. Phases Ligand Description Selectivity Profile Aq Aq USP: L Synergi Polar-RP (00 % Aqueous Stable) This ether linked phenyl column is polar endcapped and offers high cation retention capabilities to improve retention for ionized bases. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Aq Aq USP: L Synergi Fusion-RP (00 % Aqueous Stable) A low ligand density polar embedded C8, this unique phase contributes to hydrogen bonding and donating. It provides balanced selectivity for acids and bases. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Aq Aq USP: L Synergi Hydro-RP (00 % Aqueous Stable) Polar endcapped C8 column that provides very high hydrophobic interactions and hydrogen donating capabilities make this column ideal for retaining polar bases. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Synergi Max-RP Densely bonded C contributes a lot of hydrophobic retention and steric based selectivity. Combined characteristics of the base silica and the bonded phase will also provide hydrogen bonding benefits. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Material Characteristics Packing Material Particle Shape/ Size (µm) Pore Size (Å) Surface Area (m /g) Carbon Load % Endcapping ph Range Synergi Max-RP Spher * Synergi Hydro-RP Spher Hydrophilic Synergi Polar-RP Spher Hydrophilic Synergi Fusion-RP Spher * Synergi Max-RP Spher., * Synergi Hydro-RP Spher., Hydrophilic Synergi Polar-RP Spher., Hydrophilic Synergi Fusion-RP Spher., * *ph range under isocratic conditions. ph range is - 8 under gradient conditions. Important! Measurements illustrated here are not absolute, but a relative measurement to other Phenomenex columns. In this display, the individual measurements cannot be compared to each other. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

15 Unique Phases for a Different Separation The Synergi phases offer the ability to achieve greater resolution and differing peak elution order while staying in reversed phase mode. Increase retention and separation of earlier eluting polar compounds with some added polar selectivity Ether-linked Phenyl vs. C8 Polar Endcapping vs. Nonpolar Endcapping Synergi µm Polar-RP 5 6 App ID 85 mau Synergi µm Hydro-RP App ID min min mau Traditional 5 µm C8 6 App ID Traditional 5 µm C8 App ID min min Dimensions: 50 x.6 mm Mobile Phase: 0 mm Potassium phosphate, ph / Methanol (50:50) Flow Rate:.0 ml/min Temperature: Ambient Detection: 0 nm (ambient) Sample:. Metaproterenol. Pindolol. Metoprolol. Alprenolol 5. Propranolol 6. Ethyl paraben Achieve greater resolution by simply switching column phase Dimensions: 50 x.6 mm Mobile Phase: 0 mm Potassium phosphate, ph 7 / Methanol (60:0) Flow Rate:.0 ml/min Temperature: Ambient Detection: 0 nm (ambient) Sample:. Phenylephrine. Phenylpropanolamine. Pseudoephedrine. Methyl paraben Polar Embedded C8 vs. Traditional C8 Sharper peaks and alternate peak elution order Synergi.5 µm Fusion-RP min Traditional.9 µm C min 8 App ID 665 App ID 667 Dimensions: 50 x.0 mm Mobile Phase: A: 0. % Formic acid in Water B: 0. % Formic acid in Acetonitrile Gradient: A/B (95:5) to (5:95) in.9 minutes Flow Rate:. ml/min Temperature: 50 C Detection: 5 nm (ambient) Instrument: Agilent 00 SL Sample:. Pyridine. Acetaminophen. Benzyl Alcohol. Nortriptyline 5. -Methyl--Nitrobenzoic Acid 6. -Chlorcinnamic Acid 7. -Hydroxy--Methylbenzaldehyde 8. Hexanophenone Comparative separations may not be representative of all applications. Conditions are the same for both columns except where noted. See page for ordering information. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web: 5

16 World s # HPLC Column Dependable, ultra-pure silica-based HPLC columns that offer an extensive variety of selectivities which are scalable from micro-bore to preparative and purification scale solutions. Phases Ligand Description Selectivity Profile USP: L USP: L7 Luna C8() C8 phase is densely bonded to provide high hydrophobic retention and discriminating steric selectivity. endcapping reduces electrostatic based selectivity to a minimum. Luna C8() C8 column provides less hydrophobic retention than our C8, but the density of the ligand bonding creates more steric based selectivity. The C8 columns are generally better hydrogen bond acceptors, and better for acidic compounds. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 USP: L F F F F F USP: L N C USP: L0 Luna Phenyl-Hexyl ur most hydrophobic phenyl column and it will also provide good hydrogen accepting functionality for acidic retention. Luna PFP() Pentafluorophenyl groups provide very little hydrogen bonding abilities, but the strongly electronegative fluorine groups will provide good charge based selectivity for cationic compounds, while the rigid bonded phase is a good steric selector. Luna CN Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Nitrile groups bound to the silica surface offer a unique polar selectivity under reversed phase or normal phase conditions. Material Characteristics Packing Material Particle Shape/ Size (μm) Pore Size (Å) Surface Area (m /g) Carbon Load % ph Stability Luna C8() Spher., 5,0, 0-PREP, * Luna C8() Spher..5,, 5,0, 0-PREP, * Luna Phenyl-Hexyl Spher., 5,0, 0-PREP, * Luna CN Spher., 5, Luna PFP() Spher., * ph range under isocratic conditions. ph range is - 8 under gradient conditions. Important! Measurements illustrated here are not absolute, but a relative measurement to other Phenomenex columns. In this display, the individual measurements cannot be compared to each other. 6 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

17 Long Column Lifetimes and Excellent Performance Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility. Superior Particle Smoothness Agilent Technologies ZRBAX 5 µm SB-C8 Waters Symmetry 5 µm C8 Phenomenex Luna 5 µm C8 Batch Quality and Reproducibility For over 5 years, Luna has been manufactured and tested to ensure quality and reproducibility. The Phenomenex Quality Management System is meticulous, validating every batch of material according to 6 different test parameters before it is ever packed into an HPLC column. Breadth of Formats ZRBAX is a registered trademark of Agilent Technologies, Inc. Symmetry is a registered trademark of Waters Corporation. Phenomenex is in no way affiliated with Waters Corporation or Agilent Technologies. See pages - for ordering information. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web: 7

18 Speed, Dilute-and-Shoot Analysis These monolithic, silica-based HPLC columns are designed for high-throughput analysis and the direct injection of dirty or viscous samples. The highly permeable solid support allows you to run at flow rates significantly higher than those used with particle columns, without experiencing the high backpressure. Phases Ligand Description Selectivity Profile USP: L nyx C8 ctadecyl silane ligands are bound to the silica surface for strong hydrophobic selectivity. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 USP: L7 nyx C8 ctyl silane groups are bound to the silica surface with nonpolar endcapping to offer moderate hydrophobic selectivity. Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at ph.8 Material Characteristics Packing Material Macropore Size (μm) Mesopore Size (Å) Surface Area (m /g) Carbon Load % Endcapping nyx C Yes nyx C Yes nyx C8* Yes Maximum Pressure: 00 Bar; ph Range: *50 x.0 mm ID only; enhanced.5 µm macropore size for higher efficiencies Important! Measurements illustrated here are not absolute, but a relative measurement to other Phenomenex columns. In this display, the individual measurements cannot be compared to each other. Resistant to Clogging and Backpressures A dense network of macropores allow viscous samples and the mobile phase to flow throw the column less restricted in comparison to particle packed columns. This allows you to increase flow rates up to 9 ml/minute and perform worry-free dilute-and-shoot injections. Traditional Silica Fully Porous Column Monolithic Column ml/min ml/min (0 bar) App ID 550 (0 bar) App ID 55 0 min Column: nyx Monolithic C8 Dimensions: 00 x.6 mm Part No.: CH0-76 Mobile Phase: 0. % TFA in water / Acetonitrile (95/5, v/v) 0 min Flow Rate: ml/min and ml/min Detection: 0 nm Temperature: C Sample:. Maleic Acid. Fumaric Acid Conditions same for all columns except where noted. See page for ordering information. 8 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

19 Extend HPLC Column Lifetime SecurityGuard is a universal HPLC guard cartridge system designed to protect all your valuable analytical and preparative HPLC columns from the damaging effects of chemical contaminants, without altering your chromatographic results. From Injector Guard Cartridge System HPLC Column Contaminants Compounds of Interest Contaminants trapped Universal fingertight quick connection to HPLC column no wrenches required Universal Fit Patented Design Can be Easily Inspected for Contaminants Clean If it looks clean, the cartridge may be reinserted for further use. Dirty If either discoloration or particle buildup is observed, it s time to replace the cartridge. The floating outlet automatically adjust to the precise port depth of virtually any analytical HPLC column. This ensures a low-dead volume, leak-free connection, without the use of unions or adaptors. Feature applies to analytical-sized guard system only, and does not apply to SemiPrep or PREP guard cartridges. If your contaminants are colorless, replace the cartridge as often as needed to maintain chromatographic performance. See page 5 for ordering information. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web: 9

20 Gemini rdering Information For a full list of part numbers and bulk media information, please contact Phenomenex. Analytical Columns µm Microbore, Minibore and Narrow Bore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 0 x.0 0 x.0 50 x.0 00 x.0 50 x.0 50 x.0 00 x.0 50 x.0 x.0* /0pk C8 00B-9-A0 00M-9-B0 00A-9-B0 00B-9-B0 00D-9-B0 00F-9-B0 00B-9-Y0 00D-9-Y0 00F-9-Y0 AJ C6-Phenyl 00B--A0 00A--B0 00B--B0 00D--B0 00F--B0 00B--Y0 00D--Y0 00F--Y0 AJ0-79 /0pk NX C8 00M-5-B0 00A-5-B0 00B-5-B0 00D-5-B0 00F-5-B0 00B-5-Y0 00D-5-Y0 00F-5-Y0 AJ0-867 for ID:.0-.0 mm µm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 0 x.6 50 x.6 00 x.6 50 x.6 50 x.6 x.0* /0pk C8 00M-9-D0 00A-9-E0 00B-9-E0 00D-9-E0 00F-9-E0 00G-9-E0 AJ C6-Phenyl 00A--E0 00B--E0 00D--E0 00F--E0 00G--E0 AJ0-795 /0pk NX C8 00B-5-E0 00D-5-E0 00F-5-E0 00G-5-E0 AJ0-868 for ID:.-8.0 mm 5 µm Minibore and Narrow Bore Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 50 x.0 50 x.0 50 x.0 50 x.0 00 x.0 50 x.0 50 x.0 x.0* /0pk C8 00A-5-B0 00B-5-B0 00F-5-B0 00G-5-B0 00B-5-Y0 00D-5-Y0 00F-5-Y0 00G-5-Y0 AJ C6-Phenyl 00A--B0 00B--B0 00F--B0 00B--Y0 00F--Y0 00G--Y0 AJ0-79 /0pk NX C8 00A-5-B0 00B-5-B0 00F-5-B0 00B-5-Y0 00D-5-Y0 00F-5-Y0 00G-5-Y0 AJ0-867 for ID:.0-.0 mm 5 µm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.6 50 x.6 00 x.6 50 x.6 50 x.6 x.0* /0pk C8 00A-5-E0 00B-5-E0 00D-5-E0 00F-5-E0 00G-5-E0 AJ C6-Phenyl 00A--E0 00B--E0 00D--E0 00F--E0 00G--E0 AJ0-795 /0pk NX C8 00B-5-E0 00D-5-E0 00F-5-E0 00G-5-E0 AJ0-868 for ID:.-8.0 mm 5 µm Semi-Prep Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x 0 50 x 0 0 x 0 /pk C8 00F-5-N0 00G-5-N0 AJ C6-Phenyl 00G--N0 AJ0-7 /pk NX C8 00F-5-N0 00G-5-N0 AJ0-869 for ID: 9-6 mm Preparative Columns Axia Packed Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x. 00 x. 50 x. 50 x. 50 x 0 75 x 0 5 x.** 5 x µm /ea /ea C8 00B-5-P0-AX 00D-5-P0-AX 00F-5-P0-AX 00G-5-P0-AX 00B-5-U0-AX 00C-5-U0-AX AJ0-786 AJ0-808 C6-Phenyl 00D--P0-AX 00F--P0-AX 00G--P0-AX 00C--U0-AX AJ0-78 AJ µm /ea /ea NX C8 00B-5-P0-AX 00D-5-P0-AX 00F-5-P0-AX 00G-5-P0-AX 00B-5-U0-AX 00C-5-U0-AX AJ0-870 AJ µm /ea /ea C8 00B-6-P0-AX 00D-6-P0-AX 00F-6-P0-AX 00G-6-P0-AX 00B-6-U0-AX AJ0-786 AJ µm /ea /ea NX C8 00B-55-P0-AX 00D-55-P0-AX 00F-55-P0-AX 00G-55-P0-AX AJ0-870 AJ0-87 for ID: 8-9 mm 0-9 mm U.S. Patent No. 7, 56, 67 Axia Packed Preparative Columns (mm) continued SecurityGuard Cartridges (mm) Phases 00 x 0 50 x 0 50 x 0 50 x x x x 50 5 x µm /ea C8 00D-5-U0-AX 00F-5-U0-AX 00G-5-U0-AX 00B-5-V0-AX AJ0-808 C6-Phenyl 00D--U0-AX 00G--U0-AX AJ µm /ea NX C8 00D-5-U0-AX 00F-5-U0-AX 00G-5-U0-AX 00B-5-V0-AX AJ µm /ea C8 00D-6-U0-AX 00F-6-U0-AX 00G-6-U0-AX 00B-6-V0-AX 00D-6-V0-AX 00F-6-V0-AX 00G-6-V0-AX AJ µm /ea NX C8 00D-55-U0-AX 00F-55-U0-AX 00G-55-U0-AX 00B-55-V0-AX 00D-55-V0-AX 00F-55-V0-AX 00G-55-V0-AX AJ0-87 for ID: 0-9 mm If Gemini analytical columns do not provide at least an equivalent separation as compared to a competing column of similar particle size, similar phase and dimensions, return the column with comparative data within 5 days for a FULL REFUND. *SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-8 SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-70 **PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8 PREP SecurityGuard Cartridges require holder, Part No.: AJ Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

21 Synergi rdering Information For a full list of part numbers and bulk media information, please contact Phenomenex. Analytical Columns µm Microbore and Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 50 x.0 50 x.0 0 x.0 50 x.0 75 x.0 50 x.0 50 x.0 x.0* /0pk Max-RP 00B-7-A0 00F-7-A0 00A-7-B0 00B-7-B0 00C-7-B0 00F-7-B0 00G-7-B0 AJ0-607 Hydro-RP 00B-75-A0 00F-75-A0 00G-75-A0 00A-75-B0 00B-75-B0 00C-75-B0 00F-75-B0 00G-75-B0 AJ0-750 Polar-RP 00B-6-A0 00F-6-A0 00A-6-B0 00B-6-B0 00C-6-B0 00F-6-B0 00G-6-B0 AJ Fusion-RP 00B--A0 00F--A0 00A--B0 00B--B0 00C--B0 00F--B0 00G--B0 AJ for ID:.0-.0 mm µm Narrow Bore Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 50 x.0 50 x.0 50 x.0 x.0* /0pk Max-RP 00A-7-Y0 00B-7-Y0 00F-7-Y0 00G-7-Y0 AJ0-607 Hydro-RP 00A-75-Y0 00B-75-Y0 00F-75-Y0 00G-75-Y0 AJ0-750 Polar-RP 00A-6-Y0 00B-6-Y0 00F-6-Y0 00G-6-Y0 AJ Fusion-RP 00A--Y0 00B--Y0 00F--Y0 00G--Y0 AJ for ID:.0-.0 mm µm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.6 50 x.6 75 x.6 50 x.6 50 x.6 x.0* /0pk Max-RP 00A-7-E0 00B-7-E0 00C-7-E0 00F-7-E0 00G-7-E0 AJ0-607 Hydro-RP 00A-75-E0 00B-75-E0 00C-75-E0 00F-75-E0 00G-75-E0 AJ0-75 Polar-RP 00A-6-E0 00B-6-E0 00C-6-E0 00F-6-E0 00G-6-E0 AJ Fusion-RP 00A--E0 00B--E0 00C--E0 00F--E0 00G--E0 AJ for ID:.-8.0 mm Preparative Columns Axia Packed Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x. 00 x. 50 x. 50 x. 5 x.** µm /ea Max-RP 00B-7-P0-AX 00D-7-P0-AX 00F-7-P0-AX 00G-7-P0-AX AJ0-78 Hydro-RP 00B-75-P0-AX 00D-75-P0-AX 00F-75-P0-AX 00G-75-P0-AX AJ0-78 Polar-RP 00B-6-P0-AX 00D-6-P0-AX 00F-6-P0-AX 00G-6-P0-AX AJ0-785 Fusion-RP 00B--P0-AX 00D--P0-AX 00F--P0-AX 00G--P0-AX AJ µm /ea Max-RP 00D-50-P0-AX Inquire 00G-50-P0-AX AJ0-78 Hydro-RP Inquire 00G-76-P0-AX AJ0-78 Polar-RP Inquire 00G-5-P0-AX AJ0-785 Fusion-RP Inquire 00G-5-P0-AX AJ0-78 for ID: 8-9 mm Axia Packed Preparative Columns (mm) continued SecurityGuard Cartridges (mm) Phases 50 x 0 75 x 0 00 x 0 50 x 0 5 x 0.0 µm /ea Max-RP 00B-7-U0-AX 00C-7-U0-AX 00D-7-U0-AX 00G-7-U0-AX AJ0-80 Hydro-RP 00B-75-U0-AX 00C-75-U0-AX 00D-75-U0-AX 00G-75-U0-AX AJ0-805 Polar-RP 00B-6-U0-AX 00C-6-U0-AX 00D-6-U0-AX 00G-6-U0-AX AJ0-807 Fusion-RP 00D--U0-AX 00G--U0-AX AJ µm /ea Max-RP 00B-50-U0-AX 00D-50-U0-AX 00G-50-U0-AX AJ0-80 Hydro-RP 00B-76-U0-AX 00G-76-U0-AX AJ0-805 Polar-RP 00B-5-U0-AX 00D-5-U0-AX 00G-5-U0-AX AJ0-807 Fusion-RP 00D-5-U0-AX 00G-5-U0-AX AJ0-806 for ID: 0-9 mm µm Semi-Prep Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x 0 0 x 0 /pk Max-RP 00G-7-N0 AJ0-775 Hydro-RP 00G-75-N0 AJ0-75 Polar-RP 00G-6-N0 AJ0-776 Fusion-RP 00G--N0 AJ for ID: 9-6 mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-8 SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-70 **PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8 PREP SecurityGuard Cartridges require holder, Part No.: AJ0-877 If Synergi analytical columns do not provide at least an equivalent separation as compared to a competing column of similar particle size, similar phase and dimensions, return the column with comparative data within 5 days a FULL REFUND. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

22 Luna rdering Information For a full list of part numbers and bulk media information, please contact Phenomenex. Fast LC Columns.5 µm Speed Technology (HST) Columns (mm) Phase 0 x.0 50 x.0 00 x.0 50 x.0 00 x.0 Luna.5 µm C8()-HST 00A-6-B0 00B-6-B0 00D-6-B0 00B-6-Y0 00D-6-Y0 µm Microbore and Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 50 x.0 0 x.0 50 x.0 00 x.0 50 x.0 x.0* /0pk C8() 00B-8-A0 00F-8-A0 00A-8-B0 00B-8-B0 00D-8-B0 00F-8-B0 AJ0-89 C8() 00B-5-A0 00F-5-A0 00A-5-B0 00B-5-B0 00D-5-B0 00F-5-B0 AJ0-86 CN 00A-5-B0 00B-5-B0 00D-5-B0 00F-5-B0 AJ0-0 Phenyl-Hexyl 00B-56-A0 00A-56-B0 00B-56-B0 00D-56-B0 00F-56-B0 AJ0-50 NH 00F-77-A0 00A-77-B0 00B-77-B0 00D-77-B0 00F-77-B0 AJ0-0 PFP() 00A-7-B0 00B-7-B0 00D-7-B0 00F-7-B0 AJ0-86 for ID:.0-.0 mm µm Narrow Bore and Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 50 x.0 50 x.0 0 x.6 50 x.6 75 x.6 00 x.6 50 x.6 x.0* x.0* /0pk /0pk C8() 00A-8-Y0 00B-8-Y0 00F-8-Y0 00A-8-E0 00B-8-E0 00C-8-E0 00D-8-E0 00F-8-E0 AJ0-89 AJ0-90 C8() 00A-5-Y0 00B-5-Y0 00F-5-Y0 00A-5-E0 00B-5-E0 00C-5-E0 00D-5-E0 00F-5-E0 AJ0-86 AJ0-87 CN 00B-5-Y0 00F-5-Y0 00A-5-E0 00B-5-E0 00C-5-E0 00D-5-E0 00F-5-E0 AJ0-0 AJ0-05 Phenyl-Hexyl 00B-56-Y0 00F-56-Y0 00A-56-E0 00B-56-E0 00C-56-E0 00D-56-E0 00F-56-E0 AJ0-50 AJ0-5 NH 00B-77-Y0 00F-77-Y0 00B-77-E0 00D-77-E0 00F-77-E0 AJ0-0 AJ0-0 PFP() 00B-7-Y0 00F-7-Y0 00B-7-E0 00D-7-E0 00F-7-E0 AJ0-86 AJ0-87 for ID:.0-.0 mm.-8.0 mm 5 µm Microbore and Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x.0 50 x.0 50 x.0 0 x.0 50 x.0 50 x.0 50 x.0 x.0* /0pk C5 00A-0-B0 00B-0-B0 00F-0-B0 AJ0-9 C8() 00B-9-A0 00F-9-A0 00G-9-A0 00A-9-B0 00B-9-B0 00F-9-B0 00G-9-B0 AJ0-89 C8() 00B-5-A0 00F-5-A0 00G-5-A0 00A-5-B0 00B-5-B0 00F-5-B0 00G-5-B0 AJ0-86 CN 00A-55-B0 00B-55-B0 00F-55-B0 00G-55-B0 AJ0-0 Phenyl-Hexyl 00B-57-A0 00F-57-A0 00G-57-A0 00A-57-B0 00B-57-B0 00F-57-B0 00G-57-B0 AJ0-50 NH 00F-78-A0 00A-78-B0 00B-78-B0 00F-78-B0 00G-78-B0 AJ0-0 PFP() 00A-8-B0 00B-8-B0 00F-8-B0 AJ0-86 for ID:.0-.0 mm 5 µm Narrow Bore and Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 0 x.0 50 x.0 50 x.0 50 x.0 0 x.6 50 x.6 75 x.6 x.0* x.0* /0pk /0pk C5 00F-0-Y0 00B-0-E0 AJ0-9 AJ0-9 C8() 00B-9-Y0 00F-9-Y0 00G-9-Y0 00A-9-E0 00B-9-E0 00C-9-E0 AJ0-89 AJ0-90 C8() 00A-5-Y0 00B-5-Y0 00F-5-Y0 00G-5-Y0 00A-5-E0 00B-5-E0 00C-5-E0 AJ0-86 AJ0-87 CN 00B-55-Y0 00F-55-Y0 00G-55-Y0 00A-55-E0 00B-55-E0 00C-55-E0 AJ0-0 AJ0-05 Phenyl-Hexyl 00A-57-Y0 00B-57-Y0 00F-57-Y0 00G-57-Y0 00A-57-E0 00B-57-E0 00C-57-E0 AJ0-50 AJ0-5 NH 00B-78-Y0 00F-78-Y0 00G-78-Y0 00A-78-E0 00B-78-E0 00C-78-E0 AJ0-0 AJ0-0 PFP() 00B-8-Y0 00F-8-Y0 00A-8-E0 00B-8-E0 AJ0-86 AJ0-87 for ID:.0-.0 mm.-8.0 mm Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

23 Luna rdering Information (continued) Analytical Columns 5 µm Analytical and Semi-Prep Columns (mm) SecurityGuard Cartridges (mm) Phases 00 x.6 50 x.6 50 x.6 50 x 0 50 x 5 x.0* 0 x 0 /0pk /pk C5 00D-0-E0 00F-0-E0 00G-0-E0 AJ0-9 AJ-77 C8() 00D-9-E0 00F-9-E0 00G-9-E0 00G-9-N0 AJ0-90 AJ0-7 C8() 00D-5-E0 00F-5-E0 00G-5-E0 00G-5-N0 00G-5-AK AJ0-87 AJ0-7 CN 00D-55-E0 00F-55-E0 00G-55-E0 00G-55-N0 AJ0-05 AJ0-7 Phenyl-Hexyl 00D-57-E0 00F-57-E0 00G-57-E0 00G-57-N0 AJ0-5 AJ0-7 NH 00D-78-E0 00F-78-E0 00G-78-E0 00G-78-N0 AJ0-0 AJ0-76 PFP() 00D-8-E0 00F-8-E0 00G-8-E0 00G-8-N0 AJ0-87 AJ0-876 for ID:.-8.0 mm 9-6 mm Preparative Columns Axia Packed Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 50 x. 00 x. 50 x. 50 x. 50 x 0 00 x 0 50 x 0 5 x.** 5 x 0 u /ea /ea 5 µm C5 00F-0-P0-AX C8() 00B-9-P0-AX 00D-9-P0-AX 00F-9-P0-AX 00B-9-U0-AX 00D-9-U0-AX AJ0-780 AJ0-80 C8() 00B-5-P0-AX 00D-5-P0-AX 00F-5-P0-AX 00G-5-P0-AX 00B-5-U0-AX 00D-5-U0-AX 00G-5-U0-AX AJ0-789 AJ0-80 CN 00B-55-P0-AX 00F-55-P0-AX 00G-55-P0-AX 00D-55-U0-AX AJ0-80 AJ0-8 Phenyl-Hexyl 00B-57-P0-AX 00D-57-P0-AX 00F-57-P0-AX 00B-57-U0-AX 00D-57-U0-AX AJ0-78 AJ0-80 NH 00D-78-P0-AX 00F-78-P0-AX 00G-78-P0-AX 00G-78-U0-AX AJ0-86 AJ0-809 PFP() 00B-8-P0-AX 00D-8-P0-AX 00F-8-P0-AX 00G-8-P0-AX 00B-8-U0-AX 00D-8-U0-AX 00G-8-U0-AX AJ0-877 AJ0-878 for ID: 8-9 mm 0-9 mm Axia Packed Preparative Columns (mm) (continued) SecurityGuard Cartridges (mm) Phases 50 x. 00 x. 50 x. 50 x 0 50 x 0 50 x x 50 5 x.** 5 x 0 u /ea /ea 0 µm C5 00D-09-P0-AX 00G-09-P0-AX 00G-09-V0-AX C8() 00B-50-P0-AX 00G-50-P0-AX 00B-50-U0-AX 00G-50-U0-AX 00G-50-V0-AX AJ0-780 AJ0-80 C8() 00B-5-P0-AX 00D-5-P0-AX 00G-5-P0-AX 00B-5-U0-AX 00G-5-U0-AX 00B-5-V0-AX 00G-5-V0-AX AJ0-789 AJ0-80 CN 00G-00-P0-AX AJ0-80 AJ0-8 Phenyl-Hexyl 00G-85-P0-AX 00G-85-U0-AX 00B-85-V0-AX AJ0-78 AJ0-80 NH 00G-79-P0-AX AJ0-86 AJ0-809 for ID: 8-9 mm 0-9 mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-8 SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-70 **PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8 PREP SecurityGuard Cartridges require holder, Part No.: AJ0-877 If Luna analytical columns do not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase, and dimensions, return the column with comparative data within 5 days for a FULL REFUND. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

24 nyx rdering Information Columns Part No. Description Size (mm) Price Capillary Columns CH0-888 nyx Monolithic C8 50 x 0.05 CH0-766 nyx Monolithic C8 50 x 0. CH0-889 nyx Monolithic HD-C8 50 x 0. CH0-890 nyx Monolithic C8 50 x 0. CH0-89 nyx Monolithic HD-C8 50 x 0. CH0-89 nyx Monolithic C8 Trapping Column 50 x 0. CH0-89 nyx Monolithic C8 50 x 0. Analytical Columns CH0-87 nyx Monolithic C8 50 x.0 CH0-867 nyx Monolithic C8 00 x.0 CH0-86 nyx Monolithic C8 5 x.0 CH0-86 nyx Monolithic C8 50 x.0 CH0-858 nyx Monolithic C8 00 x.0 CH0-76 nyx Monolithic C8 00 x.6 CH0-76 nyx Monolithic C8 50 x.6 CH0-765 nyx Monolithic C8 5 x.6 CH0-767 nyx Monolithic C8 00 x.6 SemiPrep Columns CH nyx Monolithic C8 00 x 0.0 Guard Cartridge System KJ0-868 nyx Monolithic C8 Guard Cartridge Kit ( pk cartridges + holder + wrench) 5 x.0 CH0-869 nyx Monolithic C8 Guard Cartridges (/pk) 5 x.0 KJ0-865 nyx Monolithic C8 Guard Cartridge Kit ( pk cartridges + holder + wrench) 5 x.0 CH0-866 nyx Monolithic C8 Guard Cartridges (/pk) 5 x.0 KJ0-765 nyx Monolithic C8 Guard Cartridge Kit ( pk cartridges + holder + wrench) 5 x.6 CH0-769 nyx Monolithic C8 Guard Cartridges (/pk) 5 x.6 KJ0-765 nyx Monolithic C8 Guard Cartridge Kit ( pk cartridges + holder + wrench) 0 x.6 CH nyx Monolithic C8 Guard Cartridges (/pk) 0 x.6 Method Validation Kit KH0-765 nyx Monolithic C8 Method Validation Kit ( columns from different batches) 00 x.6 Column Coupler AQ0-765 nyx Column Coupler If nyx analytical columns do not provide at least an equivalent separation as compared to a competing column of the same monolithic characteristics, similar phase, and dimensions, return the column with comparative data within 5 days for a FULL REFUND. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

25 SecurityGuard rdering Information If HPLC Column ID (mm) is: Analytical Semi-Prep Prep Use Cartridges (mm):.0 x.0.0 x x x. 5.0 x 0.0 Material Description ph Stability Cartridges for General Purpose /0 pk /0 pk / pk /ea /ea C8 (DS, ctadecyl).5-0 AJ0-86 AJ0-87 AJ0-7 AJ0-789 AJ0-80 C (Dodecyl).5-0 AJ0-607 AJ0-607 AJ0-775 AJ0-78 AJ0-80 C8 (MS, ctyl).5-0 AJ0-89 AJ0-90 AJ0-7 AJ0-780 AJ0-80 C5 (Pentyl).5-0 AJ0-9 AJ0-9 AJ0-77 C () - 9 AJ0-98 AJ0-99 AJ0-77 NH (Amino, Aminopropyl).5 - AJ0-0 AJ0-0 AJ0-76 AJ0-86 AJ0-809 CN (Cyano, Cyanopropyl) AJ0-0 AJ0-05 AJ0-7 AJ0-80 AJ0-8 Phenyl (Phenylhexyl).5-0 AJ0-50 AJ0-5 AJ0-7 AJ0-78 AJ0-80 PFP() (Pentafluorophenyl).5-8 AJ-86 AJ0-87 AJ0-876 AJ0-877 AJ0-878 RP- (Reversed Phase - Polymer) 0 - AJ AJ AJ0-768 AJ0-858 Polar-RP (Ether-linked Phenyl).5-7 AJ AJ AJ0-776 AJ0-785 AJ0-807 Fusion-RP (C8 Polar Embedded).5-0 AJ AJ AJ AJ0-78 AJ0-806 AQ C8 (Polar Endcapped C8) AJ0-750 AJ0-75 AJ0-75 AJ0-78 AJ0-805 Gemini -NX (C8 TWIN-NX Technology) - AJ0-867 AJ0-868 AJ0-869 AJ0-870 AJ0-87 Gemini C8 (C8 TWIN Technology) - AJ AJ AJ AJ0-786 AJ0-808 Gemini C6-Phenyl (C6-Phenyl TWIN Technology) - AJ0-79 AJ0-795 Guard Cartridge Holders /kit /holder /kit /kit KJ0-8 AJ0-70 AJ0-8 AJ0-877 If the SecurityGuard Cartridge System does not provide at least an equivalent performance as compared to a competing guard cartridge system, return the product with the comparative data within 5 days for a FULL REFUND. Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web: 5

26 A New Era of Technical Support Services phenomenex.com/phenologix You already know we re just a phone call away to offer technical advice and assistance when you need it. But there s more. PhenoLogix offers in-house application development services and support for you and your lab. We provide the following services: Method Development Method ptimization Pre-validation Services Preparative and Process Scale-Up n-site Training & Consulting For more information on these services or to begin a project today, please contact your local Phenomenex representative or us at phenologix@phenomenex.com Terms and Conditions Phenomenex reserves the right to refuse sale at its discretion. Subject to Phenomenex Standard Terms and Conditions which may be viewed at Trademarks Gemini, Luna, Axia, ColumnMatch.com, MidBore, nyx, SecurityGuard, Synergi, TWIN, TWIN-NX, and are trademarks of Phenomenex, Inc. All other trademarks belong to their respective owners. Disclaimer Phenomenex is in no way affiliated with BASi Lab. Comparative separations may not be representative of all applications. Px Advanced Technology Solutions Pvt. Ltd. is a part of Phenomenex. Axia is patented by Phenomenex. U.S. Patent No. 7, 67, 8 Gemini is patented by Phenomenex. U.S. Patent No. 7, 56,67 SecurityGuard is patented by Phenomenex. U.S. Patent No. 5, 86, 8 nyx is a Phenomenex product based on monolithic technology under license from Merck KGaA, Darmstadt, Germany. 0 Phenomenex, Inc. All rights reserved. 6 Phenomenex India Tel: Fax: indiainfo@phenomenex.com Web:

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