3 How do plants and other organisms capture energy from the sun?
4 What is ATP and why is it useful in cells?
5 Plants are energy producers Like animals, plants need energy to live unlike animals, plants don t need to eat food to make that energy Plants make both FOOD & ENERGY animals are consumers (heterotrophs) plants are producers (autotrophs or photoautotrophs)
6 How do plants make energy & food? Autotrophs (plants and Cyanobacteria) use the energy from the sun to make ATP energy to make sugars glucose, sucrose, cellulose, starch, & more sun ATP sugars
7 Chemical energy and ATP Energy is available in many forms including light, heat, and electricity Adenosine triphosphate (ATP) is the principal chemical compound that cells use to store and release energy for all metabolic activities. Consists of adenine, a 5 carbon sugar called ribose, and 3 phosphate groups (these 3 phosphate groups are key to ATP s ability to store and release energy)
8 The ATP molecule
9 Storing energy Adenosine diphosphate or ADP Has 2 phosphate groups instead of ATP s 3 When a cell has energy available, it can store small amounts of it by phosphorylation (the addition of a phosphate group) to the ADP molecule, thus producing ATP
11 Releasing energy Energy is released by breaking the chemical bond between the 2 nd and 3 rd phosphates in ATP
12 ATP vs. glucose ATP is a useful source of short term energy ATP is great for transferring energy but not storing large amounts of energy for the long term A single glucose molecule stores more than 90 times the chemical energy of a molecule of ATP, therefore it is more efficient for cells to keep a small amount of ATP which can be regenerated from ADP
13 What is ATP and why is it useful in cells?
14 What happens during the process of photosynthesis?
15 Photosynthesis Overview PHOTOSYNTHESIS: A process in which plants and Cyanobacteria use the energy from sunlight to convert water and carbon dioxide into oxygen and high energy carbohydrates- such as sugar (monosaccharide) and starches (polysaccharide) Reactants: carbon dioxide and water Products: oxygen and sugar (monosaccharide- glucose). 6CO 2 + 6H 2 0 C 6 H O 2 OR Carbon dioxide + water sugars + oxygen
16 Photosynthesis sun ADP H 2 O CO 2 ENERGY building reactions SUGAR building reactions ATP sugar used immediately to synthesize sugars
17 What do plants need to grow? The factory for making energy & sugars chloroplast Fuels sunlight carbon dioxide water The Helpers enzymes CO 2 H 2 O sun ATP enzymes Make ATP! Make sugar! I can do it all And no one even notices! sugars
18 Chloroplasts are only in plants animal cells plant cells
19 What happens during the process of photosynthesis?
20 What role do pigments play in the process of photosynthesis?
21 Light and Pigments Photosynthesis requires light and chlorophyll, a molecule found in chloroplasts. Other pigments include carotenoids and anthocyanins. Energy from the sun travels to Earth in the form of light which appears white Since light is a form of energy, any compound that absorbs light also absorbs the energy from that light This light is actually made up of a mixture of different wavelengths of light- many of which are visible to your eyes
22 The wavelengths of light
23 Pigments are used to gather the sun s energy with light absorbing molecules, ex. Carotene and chlorophyll Most of the time, the green color of the chlorophyll overwhelms the other pigments, but as temperatures drop and chlorophyll molecules break down, the red and orange pigments may be seen. The principal pigment is chlorophyll Chlorophyll absorbs light very well in blue-violet and red regions but NOT the green regions. Green light is reflected (that s why plants look green) There are 2 main types of chlorophyll 1. chlorophyll a (absorbs mainly blue-violet and red regions) 2. chlorophyll b (absorbs mainly in blue and red regions)
24 Leaves Chloroplasts Leaf absorb sunlight & CO 2 sun CO 2 Chloroplasts in cell Chloroplast Chloroplasts contain Chlorophyll Chloroplast make ENERGY & SUGAR
26 Cross- section of a leaf Guard cells- are cells surrounding each stoma. They help to regulate the rate of transpiration by opening and closing the stomata, therefore helping to maintain homeostasis Stomata- Pores in the epidermis in which the exchange of oxygen and carbon dioxide in the leaf (as well as the loss of water vapor in transpiration) occurs.
27 Cross- section of a leaf The cuticle is a water-impervious protective layer covering the epidermal cells of leaves and other parts and limits water loss. The mesophyll make up the bulk of internal leaf tissue and are the major site of photosynthesis in a plant by virtue of containing large populations of chloroplast organelles
28 What role do pigments play in the process of photosynthesis?
29 What processes occur during photosynthesis?
30 Reactions of Photosynthesis Photosynthesis takes place in the chloroplast Chloroplasts contain a sack-like photosynthetic membrane called a thylakoid which are arranged in stacks called grana Proteins in the thylakoid membrane organize chlorophyll and other pigments into clusters known as photosystems (found in the light-collecting units of the chloroplast)
31 A chloroplast
32 The reactions of photosynthesis can be categorized into 2 main parts: 1.Light-dependent reaction Takes place within the thylakoid membranes Photosynthetic phosphorylation or photophosphorylation is the process of phosphate group transfer into ADP to synthesize energy rich ATP molecule making use of light as external energy source. 2. Light-independent reaction or Calvin cycle Takes place in the stroma: the space outside the thylakoid membrane
33 A chloroplast
34 Electron carriers A carrier molecule is a compound that can accept a pair of high-energy electrons and transfer them along with most of their energy to another molecule This process is called electron transport and the electron carriers themselves are known as the electron transport chain
35 NADP+ is a carrier molecule that accepts and holds 2 high energy electrons along with a hydrogen ion (H + ) form NADPH NADP electrons (2 e - ) + H + = NADPH NADPH carries these high energy electrons to chemical reactions elsewhere in the cell
37 Light-dependent reactions Requires light Uses energy from light to produce ATP, oxygen, and NADPH Water provides the electrons that are used to pump hydrogen ions (H + ) from the stroma to the thylakoid space. The electrons are then added to the NADP+ molecules to produce NADPH. The gradient produced from hydrogen ions (H+) pumped into the stroma provides the energy to make ATP. Photosynthetic phosphorylation or photophosphorylation is the process of phosphate group transfer into ADP to synthesize energy rich ATP molecule making use of light as external energy source.
38 Light-dependent reactions- Part 1 Light reactions take place in structures within the chloroplast called the grana. Grana are simply stacks of membrane-bound sacs called thylakoids. The thylakoid membrane is where light is harvested and its energy captured. The structures embedded within the thylakoid that harvest light are called photosystems; a chloroplast contains thousands of photosystems. Photosystems are made up of pigment molecules. In plants, chlorophyll is the main pigment, but accessory pigments such as the carotenoids are also present. These allow the plant to absorb a broader spectrum than just the wavelengths of light absorbed by chlorophyll.
39 Light-dependent reactions- Part 2 The light-dependent reaction of plants uses two photosystems. In the first photosystem (called Photosystem II because it was discovered second), the captured energy that reaches the reaction center chlorophyll boosts an electron to a higher orbital. That high-energy electron is picked up by an electron acceptor in the thylakoid membrane and transported through a series of proteins that make up an electron transport system. As the electron moves through these proteins, it loses energy. It then reaches a second photosystem (called Photosystem I because it was discovered first). The energy from the sun is absorbed by the pigments in this photosystem and again used to raise the energy level of the electron. Once again the electron is passed through an electron transport system giving up energy. Ultimately, this energy is converted to ATP and NADPH which will be used in the light-independent reactions.
40 Light-dependent reactions
41 Light-dependent reactions- Part 3 You may wonder what happens after the electron leaves the reaction center chlorophyll--does the photosystem stop working because the pigment molecule is missing an electron? Actually, a process called photolysis replaces these electrons in a reaction where a water molecule is split. Enzymes in the thylakoid split a water molecule into hydrogen ions and oxygen gas. Electrons are released and recharge the reaction center chlorophyll. The oxygen generated by photosynthesis is derived from this process, which is dependent on water. A plant that runs out of water runs out of a supply of electrons to keep its chlorophyll molecules "recharged." Thus you can understand the important role of water in photosynthesis.
42 Light-dependent reactions
43 Light-dependent reactions- Part 4 Electrons moving through the electron transport system proteins in the thylakoid membrane lose energy. Where does that energy go? It is used indirectly to make ATP. As the electrons energized in Photosystem II move through the first electron transport system, they power the pumping of hydrogen ions across the thylakoid membrane, against their concentration gradient. (The proton pump is a type of active transport channel). This creates a proton gradient. Remember, a hydrogen ion that has lost its electron is called a proton because it has a single proton in the nucleus of its atom. This process produces a high concentration of protons inside the thylakoid. This gradient is potential energy, just like the water stored behind a dam is potential energy. These electrons want to move back across the membrane (just as water wants to flow downstream) but are blocked because they are charged and cannot pass through the membrane without a carrier molecule. When water is released from a dam and flows across a turbine, the potential energy is converted to kinetic energy. The kinetic energy turns the turbines, generating electricity. Similarly, this high concentration gradient of protons is potential energy. There is a specific channel protein called ATP synthase in the thylakoid membrane, and this is the only molecule through which these hydrogen ions can flow back out of the thylakoid. As these hydrogen ions flow through this ATP synthase channel, their potential energy is converted to kinetic energy, and through this process ATP is generated from ADP. This process is called photophosphorylation because the energy used to add the phosphate to ADP and generate ATP comes from light. At the end of the second electron transport system, after Photosystem I, the low-energy electrons and a proton are eventually picked up by NADP + making NADPH. An electron starts its journey in a water molecule and ends up in a NADPH molecule. What does this accomplish? Where did all the energy from those electrons go? That energy is stored in ATP and NADPH and will be used to power the light-independent reactions producing the organic molecules that feed the plant.
47 Calvin Cycle or Light independent cycle RuBisCo, the enzyme, is used to catalyze the first major step in carbon fixation. It catalyzes RuBp. Uses ATP and NADPH to produce sugars due to the action of ATP synthase and the proton pump
48 Light- independent reaction or Calvin Cycle
49 What processes occur during photosynthesis?
50 What factors affect photosynthesis?
51 Factors affecting photosynthesis Factors below can affect photosynthesis such as 1. water availability- a shortage of water can slow or even stop photosynthesis 2. temperature- between 0 o C and 35 o C are ideal due to enzymatic functions. 3. Intensity of light- increasing can speed up photosynthesis when all other conditions are met
52 What factors affect photosynthesis?
53 Energy cycle sun Photosynthesis plants CO 2 H 2 O glucose sugars O 2 animals, plants Cellular Respiration The Great Circle of Life! Mufasa? ATP
54 Respiration How are they connected? glucose + oxygen carbon + water + energy dioxide C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 O + ATP Photosynthesis carbon dioxide + water + sun energy glucose + oxygen 6CO 2 + 6H 2 O + light C 6 H 12 O 6 6O energy + 2
55 Another view capture light energy sun Photosynthesis producers, autotrophs synthesis CO 2 waste H 2 O waste organic molecules food O 2 waste consumers, heterotrophs Cellular Respiration digestion release chemical energy ATP
56 Got the energy Ask Questions!!
57 What factors affect photosynthesis?
58 How do plants and other organisms capture energy from the sun?
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