Effect s of Special Formulated Fertilizer Synergist on Endogenous Phytohormones in Rice under Drought Stress

Transcription

1 ( Chinese J Rice Sci), 2005, 19 (5) : http :/ / www. ricescience. org 417 1, 3 1, ( 1, ; 2, ; 3, E2mail : net) Effect s of Special Formulated Fertilizer Synergist on Endogenous Phytohormones in Rice under Drought Stress XIAO Lang2tao 1, 3, WAN G Shao2xian 1,2, PEN G Ke2qin 1, XIA Shi2tou 1, CAO Yong 1, L IU Hua2ying 1 ( 1 H unan Provincial Key L aboratory of Phytohormones, H unan A gricultural Universit y, Changsha , China; 2 Technical Center of Chang de Ci garette Factory, Chang de , China; 3 Corres ponding author, E2mail : lt x net) Abstract : Pot experiments with middle2season rice variety Peiliangyou 93 served as material were conducted to study the effects of synergist added to special formulated fertilizers on endogenous phytohormones ABA, IAA, GA3 and ZR in rice plant under soil drought conditions. After drought treatment, ABA accumulated massively first and then decreased. ABA in roots and leaves rose sharply, 58 to 101 times as high as that in the control in roots. ABA accumulation in root was much faster than that in leaf. Synergist treat ment enhanced the accumulation of ABA. Before drought stress, the contents of IAA, ZR and GA3 in roots under synergist treatment were higher than those of the control, while under drought stress, the contents of IAA, ZR and GA3 in roots decreased, and the decreasing rates of synergist treatment were larger than those of the control. However, the changes of IAA, ZR and GA3 contents in leaf were contrary. Key words : rice ; drought stress ; formulated fertilizer synergist ; endogenous phytohormones :, 93, (ABA) ( IAA) ( GA3 ) ( ZR),ABA, ABA, ABA , ABA ABA, ABA, IAA ZR GA3 ;,, IAA ZR GA3 : ; ; ; : Q ; S ; S311 ; S : A : (2005) ,,, [1 4 ],,,, [5,6 ], ABA, ABA, [7 9 ],, ABA, ABA,,, [10,11 ],,,,, 3 [12 ], ( IAA ZR GA3 ABA),, : : ; : : ( ),,,

2 418 ( Chinese J Rice Sci) 19 5 ( ) p H 6. 5, g/ kg, g/ kg, g/ kg, g/ kg, g/ kg, g/ kg, g/ kg : 93, ,5 30 : %(N 18 % P % K 8. 3 %) ( 2 %,N 18 % P % K 8. 3 %),, [12 ] , 12 kg, 3, 1, 2, 6, : 1), 3. 4 g ;2), 3. 4 g ( : 1 hm kg, 675 kg ) 7 5,,,,,, ( IAA ZR GA3 ABA) : ( H PL C ) [13 ] : Aglient 1100,,, IAA GA3 ZR ABA Fluka : Waters C18 (46 mm i. d 250 mm,5 m) ; 35 ; ( ) ; 10 ml/ min ; 20 L ; 254 nm ; : ( ) g, g, 4 80 % 11 ml (5 ml 2 ml 2 ml 2 ml ),, 4 (15 h, ), g 10 min,,,,, 8 ml (0. 1 mol/ L, p H 9. 0) ( g, 20 min), ( PV PP) (D EA E Sep hadex A225), Sep2Pak C18 ( Waters ), 50 %( ) ,,,, ( GA3 ), GA3, ( 1), GA3 3 d, GA3, GA3, %,, GA3, 3 6, GA3 11 d, GA3, 5, GA3 40 % GA3 GA3 ( 1), GA3 3 d, GA3, GA , GA3, %, GA d, 20 %,, ( ZR) ZR,,, ( 2), ZR g/ g, 1 Table 1. Variation of water content in the soil. % Treat ment CK Synergist

3 : Fig. 1. Effects of synergist on GA3 content in rice plant under natural soil drought conditions. 2 Fig. 2. Effects of synergist on ZR content in rice plant under natural soil drought conditions. ZR d, ZR, ZR g/ g, %, ZR,, 5 11 d, ZR, ZR, % ZR,, ZR ( 2), ZR 1, g/ g ( g/ g), ZR g/ g,, 3 d, ZR,, 2. 3, 2, ZR, ZR %, 90 % 7 16, ZR, 2. 7, ( IAA) IAA,, GA3 ZR ( 3), IAA 2, 3 d, 7 8,, 2. 0 g/ g,iaa, 4 11 d, IAA,, 70 %, % IAA GA3,, ( 3) IAA, IAA (ABA),, ABA, ( 4), ABA, g/ g 3 d, ABA ABA 58, ABA 1 0 1,

4 420 ( Chinese J Rice Sci) 19 5 ( ) 3 Fig. 3. Effects of synergist on IAA content in rice plant under natural soil drought conditions. 4 Fig. 4. Effects of synergist on ABA content in rice plant under natural soil drought conditions. ABA, g/ g, 2. 5, g/ g, , 11 d, ABA, ABA, ABA,, ( 4), GA3 ZR, ABA, 3 d, ABA, 9, ABA g/ g, 5. 2,ABA, % % 11 d, ABA,, ABA,,, ABA, ABA [7 10 ] ABA,,,,,, [10,14 ],,,, ;, [11 ],, ABA, , ABA 4 9,,,

5 : 421,,,,, IAA, GA3, GA3 [5 ],, IAA GA3 ZR,, 3,,,,,, ZR GA3 IAA, : 1 Parry M A J, John A P, Shahnaz K, et al. Rubisco activity : effect s of drought stress. Ann B ot, 2002,89 : Griffit hs H, Parry M A J. Plant responses to water stress. Ann B ot, 2002,89 : Wang Z L, Mambelli S, Setter T L. Abscisic acid catabolism in maize kernels in response to water deficit at early endosperm de2 velopment. Ann B ot, 2002, 90 : Yu L X, Setter T L. Comparative transcriptional profiling of pla2 centa and endosperm in developing maize kernels in response to water deficit. Plant Physiol, 2003, 131 : ,,,.., 2002,39 (2) : Chaves M M, Pereira J S, Maroc J O, et al. How plant s cope with water stress in the field? Photosynt hesis and growth. Ann B ot, 2002, 89 : Jia W S, Wang Y Q, Zhang S Q, et al. Salt2stress induced ABA accumulation is more sensitively triggered in root s t han in shoot s. J Ex p B ot, 2002, 53 : Borel C, Audran C, Frey A, et al. N. plumbagini f olia zeaxan2 thin epoxidase transgenic lines have unaltered baseline ABA ac2 cumulations in roots and xylem sap, but contrasting sensitivities of ABA accumulation to water deficit. J Ex p B ot, 2001, 52 : Sharp R E, Lenoble M E. ABA, et hylene and t he control of shoot and root growth under water stress. J Ex p B ot, 2002, 53 : ,,,. ABA.,2003,39 (5) : Hare P D, Cress W A, Staden J V. Proline synt hesis and degra2 dation : a model system for elucidating stress2related signal trans2 duction. J Ex p B ot, 1999, 50 : ,,,..,2003,9 (3) : ,,,.., 2002, 20 (2) : Sauter A, Davies W J, Hartung W. The long2distance abscisic acid signal in t he droughted plant : t he fate of t he hormone on it s way from root to shoot. J Ex p B ot, 2001, 52 : ,,,, 16, 64,, 8, , ( ) : 35 ; : ; : ; : ; :