Strength in Technology. Fortis Phase Chemistry Guide

Transcription

1 Strength in Technology Fortis Phase Chemistry Guide

2 Fortis BIO 22 page 23.indd 7//23 :35:42 Other Product Guides Available Fortis Phase Chemistry Selectivity Fortis SpeedCore - Modern Core-Shell technology - High speed separations General UHPLC use Method Development from ph -2 Acids Bases Neutrals Fortis H2o Polar endcapped Increased polar retention Hydrophilic analytes Organic acids Catecholamines New Core-Shell Technology Strength in Technology.7µm Fortis UHPLC Columns.7µ Fortis UHPLC columns - High Efficiency UHPLC particles - 8 Stationary phases for high resolution Fortis Diphenyl Unique di-phenyl structure Metabolite profiling Separate positional isomers Metabolites Positional Isomers Hydrophilic / Hydrophobic analytes Ultra High Pressure Chromatography Fortis C8 General UHPLC use Method Development Lipids Steroids Highly Hydrophobic analytes Improve your UHPLC Performance Includes.7µ FortisBIO UHPLC Columns 8 Chemistry Choices Increase Efficiency Increase Speed Improve Resolution Greater Sensitivity Lower Backpressure FortisBIO Strength in Technology Peptide & Protein Columns Strength in Technology FortisBIO - Peptide and Protein columns (3Å pore size) -.7µ for ultra high resolution separations Fortis HILIC High polar retention Homogenous silanol concentration Improve MS sensitivity Fortis HILIC Diol Alternate selectivity to bare silica Stable bonding HILIC or Normal phase mode Carboxylic acids Nucleotides Vitamins Steroids Proteins Metabolites HPLC/UHPLC Columns UniverSil - Economical alternative to older type B silicas - Excellent reproducibility Fortis Cyano Cyano functionality Reversed phase or Normal phase Explosives Pesticides Steroids Fortis Amino Reproducible, Robust bonding Reversed phase, Normal phase or Ion exchange mode Saccarides Oligonucleotides Steroids Tel: Tel: Product Guide 3

3 Si O Si O Si O Si O Si O Si O Si O Si O Si Fortis Method Development Options Choice of Stationary phase functionality Based on Ultra pure silica Reversed Phase (RP) and Normal Phase (NP) options H2o - Polar endcapped C8 - Increased polar retention - Organic acids - Catecholamines Diphenyl - Unique di-phenyl structure - Separate Positional Isomers - Metabolite profiling Method Dev Getting Started : Method development typically starts with a C8 or C8 column, both provide Hydrophobic retention with good peak shapes for neutral, acidic and basic analytes. Generally if retention of polar molecules is also needed then a polar endcapped stationary phase such as Fortis H2o is a good starting choice. If selectivity is insufficient then Diphenyl or Cyano stationary phases are a good alternative, they will change selectivity and even elution order since they work on dipole characteristics as opposed to just hydrophobicity. Fortis Cyano is good in normal phase (NP) conditions for polar analytes with CO, NH2, NHR2 or NR2 groups. If small polar molecules still do not retain then HILIC chromatography is a suitable alternative H2o Extra Polar Retention Diphenyl Change Selectivity Method Dev P P Si Si O O Si O O Si O Si O Si O Si Si O Si O Si O Si O Si Si Si Si O Si O Si O Si O Si O Si O Si O Si O Si O Si C Si 5 5 HILIC - High Polar Retention - Highly Pure Silica - Carboxylic acids - Nucleotides CN O Si O Si O Si O O Si C8 - General HPLC use - Method dev. from ph -2 - Acids, Bases and Neutral Si O Si O Si O Si O Si O Si O Si O Si O Si Si O Si O Si O Si O Si O Si O Si O Si O Si O O HILIC DIOL - High Polar Retention - Highly Pure Silica - Nucleotides Cyano Extra Selectivity C8 Lower Hydrophobicity CN Si CN O Si O Si O Si O Cyano - Cyano functionality - RP or NP use - Explosives - Pesticides Si O Si O Si O Si O Si NH2 Amino - High Polar Retention - Highly Pure Silica - Carbohydrates NH2 NH2 C8 - Reduced Hydrophobicity - Lipids - Steroids Tel: Tel: Acidic, Neutral & basic analytes - - Fortis C8 - Fortis Diphenyl Polar basic molecules - operated at high ph - Fortis Diphenyl - Fortis H2o Polar acidic molecules - Fortis H2o - Fortis HILIC - Fortis Cyano in NP mode Alternate Selectivity - Fortis Diphenyl - Fortis Cyano

4 C8 Extended operating ph range Superior Peak Shapes ph Range -2 Based on Ultra Pure Silica Fully Scalable - UHPLC to Prep.7μ 2.5μ 3μ 5μ μ C8 7% Column: x4.mm 3μ p/n: F8-553 Mobile Phase: A - H 2 O +.% Formic acid B - ACN +.% Formic acid Gradient: - 5% in min ml/min Inflammation and Infection ph is a pure silica based stationary phase with unique high and low ph performance. Whether carrying out simple compound screens or complex metabolite identification will provide the best in peak shape, resolution and extended ph range for method development flexibility. Optimised Peak Shape L 38m2/g Temp: 2 C Wavelength: 254nm. Dexamethasone 2. Neomycin Sulphate 3. Acetic acid Whatever the compound functionality the optimised hydrophobic bonding of Fortis C8 leads to peak symmetries being near perfect whatever the analyte type. Basic, Acidic and Neutral analyte performance is first class across the ph spectrum. Superior Peak Shapes - Higher Efficiencies Excellent Reproducibility Column: Fortis C8 5x4.mm 5μ Luna C8(2) 5x4.mm 5μ Mobile Phase: A - H 2 O +.% Formic acid B - ACN +.% Formic acid Gradient: 25-4% in min ml/min Temp: 2 C Wavelength: 254nm. Protriptyline 2. Nortriptyline 3. Amitriptyline 4. Trimipramine Fortis C8 Luna C8(2) Column: 5x4.mm 5μ p/n: F8-535 Mobile Phase: A - 5mM NH 4 OAc B - ACN Gradient: - 4% in min ml/min Temp: 2 C Wavelength: 254nm. Quetiapine Antipsychotic - ph Extreme ph range has the ability to not only operate at low ph like other silica based phases, but also to operate at high ph like hybrid phases to aid with basic analyte retention and performance. C8 ph. Lidocaine Asy -.97 N - 2,225 Rs Column: 5x3.mm 3μ p/n: F Selective Estrogen Modulator The ability to quickly equilibrate from formic acid or TFA into ammonia or bicarbonate aids in method development. Mass transfer, loadability and precision of a silica matrix are all maintained. Higher Efficiency than Hybrids - Excellent Reproducibility Retain Polar Basic Analytes Gemini C8 ph. Lidocaine Asy -.22 N- 7,85 Rs Mobile Phase: 3:7 H 2 O + mm ammonium bicarbonate : Me.4ml/min Temp: 25 C Wavelength: MS Detection Raloxifene Glucuronides 5 ph 2 5 Columns: 5x4.mm 3μ Mobile Phase: 5:5.% NH 3 : MeCN.ml/min Temp: 25 C Wavelength: 23nm Data Courtesy of : Pharmaceutical company, USA Tel: Tel:

5 High ph Stability C8 Optimised Resolution C8 The unique bonding of enables stability at extremes of ph to be maintained. Run continuously in.% ammonia shows no deterioration in efficiency over a 9 day period. 2 8 % of original Efficiency 4 Only by optimising all factors of stationary phase design can the analyst be assured of the best possible chromatography. s unique bonded character ensures that not only is reproducibility and robustness assured, but also that resolution is of the highest level. Only by obtaining sharp peak shapes for many analyte types both polar and non polar can this sort of resolution be achieved..e5 5.5e5 5.e5 4.5e5 4.e5 3.5e5 3.e5 2.5e5 35 Pesticide Transitions Column: Fortis C8 5x2.mm 3μ 2 Day Day Day 2 Day 3 Day 4 Day 5 Day Day s running at ph. Day 7 Day 8 Day 9 Analysed here are 35 transitions of pesticide residue from an apple matrix. Good LC resolution leads to excellent sensitivity in MS detection. Polar organophosphates such as Acephate and Methamidophos are retained well due to the high surface area of the phase. 2.e5.5e5.e5 5.e XIC of +MRM (35 pairs): 84./43. amu 5 from Sample 2 (Run39558_2) of Data39558.wiff (Turbo Spray) 5 2 Max..4e5 cps e5 4.e5 Advantages of Hydrophobicity 4.4e5 4.2e5 4.e5 Column: Atlantis dc8 C8 5x2.mm 3μ 3.8e5 high surface area combined with the optimised C8 ligand bonding provides high retention for compounds. This is advantageous in a number of ways: - Higher retention of analytes, more organic modifier can be used to elute, therefore greater MS sensitivity. - Higher retention of analytes, more organic leads to shorter dry-down in fraction collection. Fortis C8 Sunfire C8 Luna C8(2) Zorbax Eclipse XDB-8 Synergi Max-RP 3.e5 3.4e5 3.2e5 3.e5 2.8e5 2.e5 Column carryover of thiabendazole 2.4e5 2.2e5 XIC of +MRM (35 pairs): 22./75. amu from Sample (Job372_2_4) of DataTBZ_carryover2.wiff (Turbo Spray), Smoothed, Smoothed 2.e e5 4.e5.8e5 3.8e5 3.e5.e5 3.4e5.4e5 3.2e5 3.e5.2e5 2.8e5 2.e5.e5 2.4e5 8.e4 2.2e5 2.e5.e Max. 4.2e5 cps. XIC of +MRM (35 pairs): 22./75. amu from Sample (Run39558_) of Data39558.wiff (Turbo Spray), Smoothed, Smoothed 3.3e5 3.2e5 3.e5 2.8e5 2.e5 2.4e5 2.2e5 2.e5.8e5.e5.4e5.2e Higher retention of analytes, more chance of resolution Gemini C8 Ace 5C8 Xterra MS C k pentylbenzene Thiabendazole can be bound on the column from one gradient cycle to the next, the optimised hydrophobicity of means that carryover on column is greatly reduced since there is no secondary silanol activity to bind with analytes e5 4.e4.e5.e5.4e5 8.e4 2.e e5.e4.e e e4 25 Time, min.e4 2.e4 4.e4 2.e4 Thiabendazole.9% Column Carryover C Time, min Thiabendazole.9% Column Carryover Atlantis dc Time, min Intensity, cps Intensity, cps Data Courtesy of : Central Science Laboratories, UK Time, min Time, min Tel: Tel: Fortis column.9%

6 C8 C8 Analyte Loading Selectivity and Peak Shape Comparison Based on a silica template has high loading capability for those wishing either scale up to preparative separation or needing to load in order to correctly identity low level components. Having a 38m2/g surface area means that the phase chemistry will not overload causing poor peak shapes. This can be especially important in biological work where a high concentration of matrix interference is also often present Fortis C8 3µm 98 bar Loss in N = 3% Loss in N = 4% Loss in N = 2% x TIC MRM (** -> **) pst_fa_fortis.d + MRM_FA_Fortis.d Asy -.5 Fortis C8 3µm Asy -.97 Asy -.9 Column: x2.mm 3μ Mobile Phase: A - H 2 O +.% formic acid B - ACN Gradient: Temp: 3 C Wavelength: 2-35%B in 2min 35-4% in 5min 4-5% in 3min 5-9% in min MS Detection Smaller surface area phases and solidcore-shell particles can suffer from lower loading capability and potentially higher backpressure. Overload can be viewed as loss of efficiency and/or peak shape. Column: 5x3.mm Mobile Phase: H 2 O +.% formic acid : ACN.ml/min Temp: 3 C Diphenhydramine.2,.2,.5 & mg/ml Selectivity of C8 - Plant Hormones All C8 chemistries are capable of providing different selectivity. Selectivity can be just as important as efficiency, here we see radically different peak shapes and resolution regardless of C8 particle size for some plant hormones. Fortis C8 3µm KI GA3 Kinetix C8 2.µm 73 bar Loss in efficiency figures are in relation to.2mg/ml injection (Blue Trace). BA Loss in N = 4% Loss in N = 45% Loss in N = 22% IAA ABA x Counts vs. Acquisition Time (min) + TIC MRM (** -> **) pst_fa_inertsil.d + MRM_FA_Inertsil.d Asy -.23 Inertsil ODS-4 3µm Asy -.3 Asy Zopiclone 2. Diazepam 3. 7-Aminoflunitrazepam 4. Nitrazepam 5. Desmethyldiazepam. 7-Aminonitrazepam 7. -Hydroxy-midazolam 8. Midazolam 9. Clonazepam. Flunitrazepam. Alprazolam 2. Zolpidem 3.Oxazepam 4. 7-Aminoclonazepam Column: 5x2.mm Mobile Phase: A - H 2 O +.% formic acid B - Me +.% formic acid Gradient: -4% in 3min Temp: 3 C Zorbax SB-C8.8µm IAA Counts vs. Acquisition Time (min) Wavelength: MS Detection GA3 BA ABA Data Courtesy of : Major Pharmaceutical, Norway KI = Kinetin BA = Benzyladenine IAA = Indol-3-yl acetate ABA = Abscisic acid GA3 = Gibberellin acid KI F8-23xx F8-25xx F8-27xx - Column Diameter 3. F8-33xx F8-35xx F8-37xx - 4. F8-53xx F8-55xx F8-57xx F8-59xx Guards Length Column Diameter 2. DC8-2xxG 4. DC8-5xxG Data Courtesy of : Kings College, UK Replace xx - for.7µm - 2 for 2.5µm - 3 for 3µm - 5 for 5µm - for µm Tel: Tel:

7 Fortis Diphenyl Diphenyl Unique Selectivity Separate Positional Isomers No MS bleed, Stable Hydrophobic Ligand Enhanced Polar Retention Fortis Diphenyl is designed to provide characteristics which will enhance selectivity. It provides the analyst with extra retention of compounds containing aromatic functionality. Extra selectivity and retention can be found for polar substrates, along with metabolite profiling. Fortis Diphenyl is now available in.7µm particle size for UHPLC. Unique Functionality Fortis Diphenyl is based upon a unique di-phenyl functionality. Three controlled mechanisms of interaction can occur. This allows for unique resolution of closely related species, and metabolites. No complex mobile phases are necessary simplifying method development. Steric Selectivity (diphenyl arrangement).7μ 2.5μ 3μ 5μ L π-π (phenyl rings) Hydrophobicity (alkyl chain) Diphenyl 3% 38m2/g Metabolite Profiling Fortis Diphenyl s extended selectivity leads to its ability to discriminate between very closely related species, such as those often associated as metabolites or excipiants. The stationary phase s three modes of interaction allow subtle changes in positional spacing, loss or gain of an atom or functional group to be differentiated and separation to be achieved. Separate Positional Isomers Selectivity of compounds normally difficult to resolve on a hydrophobic alkyl chain stationary phase is simplified by the π-π interactions provided by the phenyl functionality. In this application two hydroxyestradiol steroids exhibit resolution from each other, which is not achievable on alkyl chain phases. No complex mobile phases are necessary. Isomer Selectivity Metabolite Resolution Alternate Selectivity Column: Fortis Diphenyl 5x4.mm 5μ p/n: FPH-575 Mobile Phase: 4: H 2 O : Me ml/min Temp: 2 C Wavelength: 2nm. 4-Hydroxyestradiol (mw=288.38) 2. 2-Hydroxyestradiol (mw=288.38) H O H O H O CH 3 H H H CH 3 H H H Fortis Diphenyl π-π High Selectivity Resolution Enhanced Sharp Peak Shapes - Highly Stable Diphenyl Ligand PET Tracer - PK (.) 39. (.). Dechlorinated PK95 2. PK95 Antiarrhythmic Quinidine 2. Dihydroquinidine 4 Diphenyl vs C8 Selectivity 2 Selectivity of the Fortis Diphenyl is radically different to that of a C8 stationary phase. Fortis Diphenyl In this pharmaceutical mixture we can see an increase in retention of the parent drug, whilst the degradents are all eluted quickly, removing them from co-elution with the parent Selectivity such as this can be extremely useful, combined with the ability to separate closely related species such as metabolites and positional isomers. Fortis C8 5 2 Column: Fortis Diphenyl 5x4.mm 5μ p/n: FPH-575 Mobile Phase: 4 : H 2 O : ACN ml/min Temp: 25 C Wavelength: MS Detection Data Courtesy of : Wolfson Molecular Imaging Centre Column: p/n: Mobile Phase: ml/min Temp: 25 C Wavelength: 235nm Fortis Diphenyl 5x4.mm 5μ FPH : 3 H 2 O +.% formic acid Me 8 Data Courtesy of : Major Pharmaceutical company, USA Tel: Tel:

8 Diphenyl Diphenyl Effect of Mobile phase choice Phenyl phase bleed Fortis Diphenyl Choice of mobile phase can be very important in a running a phenyl column. Whilst many people have standardised upon ACN as the organic modifier of choice, Me is a better choice in order to let the π-π interactions occur on the phenyl rings. Using ACN can not only suppress retention but also selectivity. It can be seen how maximum retention and resolution is obtained on Fortis Diphenyl in Me mobile phase, even greater than C8. Once the organic modifier is substituted for ACN not only is resolution reduced but also a large amount of retention is lost in relation to that lost on a C8. Rs -.2 Fortis C8 Me M in u t e s Fortis Diphenyl Me Due to the chemical nature of the charge on a phenyl ring, when placed in close proximity to a silica surface it does not tend to be a very stable bond. As the phenyl ring contains a chromaphore, UV baselines could be seriously affected if the bonding is not stable. Fortis Diphenyl is a more stable bonding process since the alkyl chain ligand removes the dipolar phenol/silica interactions. No observable MS-bleed Clean baselines No sample contamination Fortis Diphenyl Commercial Phenyl Phase Bleed Fortis Diphenyl Rs -.78 Data Courtesy of : Major Pharmaceutical company, UK M in u t e s Fortis C8 ACN Fortis Diphenyl FPH-23xx FPH-25xx FPH-27xx - Column Diameter 3. FPH-33xx FPH-35xx FPH-37xx - 4. FPH-53xx FPH-55xx FPH-57xx FPH-59xx Replace xx - for.7µm - 2 for 2.5µm - 3 for 3µm - 5 for 5µm - for µm Fortis Diphenyl Guards Length Column Diameter 2. DCPH-2xxG 4. DCPH-5xxG M in ut e s Fortis Diphenyl ACN Tel: Tel:

9 H2o C8 Fortis H2o Retention of Polars by Polar Endcapping Group Enhanced Resolution % Aqueous Compatible Fully Scalable Fortis H2o is designed to aid in the separation and retention of polar analytes. Complex mobile phase systems can be bypassed if sufficient retention can be provided by the stationary phase chemistry. Fortis H2o is designed to supply additional interaction with polar molecules which allows their successful retention. Retention of Polar analytes - Amino Acids.7μ 3μ 5μ μ Polar Endcapping L 8% 38m2/g Reduced Hydrophobicity over C8 Excellent Peak Shapes Fully Scalable Fortis C8 is designed to provide characteristics similar to but specifically for situations where less hydrophobicity is required. The same gains in peak shape, efficiency, resolution and scaleability are available providing increased productivity to the analyst. Optimised Peak Shape.7μ 3μ 5μ μ L7 C8 38m2/g 3% Fortis C8 Column : p/n :. L-Aspartic acid 2. L-Glutamic acid 3. Asparagine 4. L-Serine 5. L-Histidine. Glycine 7. L-Threonine 8. L-Alanine 9. L-Arginine. L-Gly-Tyr. L-Tyrosin Fortis H2o 5x2.mm 5μ FHO L-Valine 3. L-Methionine 4. L-Norvalin 5. L-Tryptophan. L-Phenylalanine 7. L-Isoleucine 8. L-Ornithine 9. L-Leucine 2. L-Lysine 2. Sarcosin 22. L-Proline Amino Acids Fortis C8 is optimised to provide the best possible peak shapes and efficiency. Basic, Acidic and Neutral analyte performance is first class. Higher Efficiencies Greater Reproducibility Symmetrical peak shapes - Lower Hydrophobicity Column: Fortis C8 5x4.mm 5μ p/n: F8-575 Mobile Phase: A - H 2 O +.% Formic acid B - Me +.% Formic acid Gradient: - 9% in min ml/min Temp: 25 C Wavelength: 254nm. Lamotrigine Bipolar disorders Data Courtesy of : Major Pharmaceutical Company, Austria Alternative Selectivity - Steroids Anticonvulsant Antiplatelet Fortis H2o s unique bonded character ensures that not only is reproducibility and robustness assured, but also that resolution is of the highest level. Different selectivity can also be achieved from that of our stationary phase DAD C, Sig=25, Ref=3,2 (TESTOST\TESTO2.D) H2o Column: Fortis C8 5x4.mm 5μ p/n: F8-575 Mobile Phase: 4 : H 2 O +.% formic acid ACN ml/min Temp: 25 C Wavelength: 22nm Column: Fortis C8 5x4.mm 5μ p/n: F8-575 Mobile Phase: 25 : 75 H 2 O : ACN ml/min Temp: 25 C Wavelength: 254nm Column: p/n: Mobile Phase: Fortis H2o 5x2.mm 3μ FHO-273.2ml/min Temp: 25 C Wavelength: DAD DAD C, Sig=25, Ref=3,2 (TESTOST\TESTO27.D) C8. Valproate Semisodium. Clopidogrel Data Courtesy of : AstraZeneca, UK Tel: Tel:

10 Cyano HILIC Fortis Cyano Retention of Polars Alternative Selectivity Normal Phase or Reverse Phase system Rapid Equilibration Fortis Cyano allows the use of aqueous reversed phase conditions to provide less retention for compounds too heavily retained on C8 functionality. However, it can also be used in normal phase solvent systems to retain and separate polar analyte species. Cyano columns are particularly useful for polar species. Fortis Cyano is now also available in.7µm particle size for UHPLC work. Herbicides.7μ 3μ 5μ L Cyano 38m2/g 7% Retention of Polar Compounds Increased MS Sensitivity Alternate Selectivity Reduced Extraction (SPE) and Dry Down Times. Fortis HILIC (Hydrophilic Interaction Chromatography) is designed to aid in the separation and retention of very polar analytes. Extended retention is afforded by the partitioning, ion-exchange and hydrogen bonding that can occur on a HILIC stationary phase. Fortis HILIC can increase sensitivity in MS analysis and provide alternate selectivity to that achieved with reversed phase C8. Fortis HILIC is now also available in.7µm particle size for UHPLC work. Polar retention in HILIC mode.7μ,3μ, 5μ L3 HILIC 38m2/g na Fortis HILIC Fortis Cyano is optimised not only to help retain and resolve polar analytes, but also to be complimentary in resolution to other Fortis phases. Normal phase as well as Reversed phase use Alternative Selectivity Rapid Equilibration Fortis Cyano Column : Fortis Cyano 5x2.mm 3μ p/n : FCN-233 Mobile Phase: 8:2 H 2 O : ACN +.2% Acetic acid Flow :.2ml/min Temp : 2 C Wavelength: 28nm. Banvel 2. Internal Std 3. 2,4-D 4. MCPA 5. PCOC. 2,4-DCP 7. 2,4-DP 8. CMPP 9. 2,4-DB. MCPB FCN-23xx FCN-25xx FCN-27xx - Column Diameter 3. FCN-33xx FCN-35xx FCN-37xx - Herbicides Fortis Cyano Guards Length Column Diameter 2. DCCN-2xxG 4. DCCN-5xxG Fortis HILIC is optimised to help retain and resolve polar analytes. By use of high concentrations of organic solvent polar analytes partition with the stationary phase. Polar Retention Alternative Selectivity Rapid Equilibration Hydrophilic Interaction Chromatography (HILIC) works in a similar way to normal phase chromatography. A polar surface combined with a non-polar mobile phase, typically ACN, allows for partition of the polar analytes and hence retention and separation. Water is used in low concentration as the strong solvent in order to elute the compounds. Usually no more than 2%-3% water is needed in order to elute most analyte species. 8 k 4 2 Adenine Theobromine Uracil Toluene Nucleosides % organic :2 ACN: Water 9: ACN: Water FCN-53xx FCN-55xx FCN-57xx FCN-59xx 4 4 Replace xx - for.7µm - 3 for 3µm - 5 for 5µm :5 ACN: Water Tel: Tel:

11 HILIC DIOL Fortis HILIC Melamine Contamination Melamine has been adulterated into many products, but most importantly into baby milk in order to increase the apparent protein content. Due to its highly polar organic nature,,3,5-triazine structure, it can be very difficult to retain in HPLC. HILIC provides a simple method in order to quickly quantitate melamine. Column : Fortis HILIC x2.mm 3μ p/n : FHI-253 Mobile Phase: 9: ACN : 2mM NH 3 OAc Flow :.2ml/min Temp : 2 C Wavelength: 2nm Nucleosides Milk Blank Spiked Milk Retention of Polar Compounds Increased MS Sensitivity Alternate Selectivity Reduced Extraction (SPE) and Dry Down Times. Fortis HILIC DIOL (Hydrophilic Interaction Chromatography) is designed to aid in the separation and retention of very polar analytes. Extended retention is afforded by the partitioning, ion-exchange and hydrogen bonding that can occur on a HILIC stationary phase. Fortis HILIC DIOL can increase sensitivity in MS analysis and provide alternate selectivity to that achieved with reversed phase chemistries. Fortis HILIC DIOL is also available in.7µm particle size for UHPLC work. 5-Fluorouracil / Uracil Fortis HILIC DIOL is optimised not only to help retain and resolve polar analytes, but also to be complimentary in resolution to other Fortis phases. Hydrophilic Interaction Mode Strong Polar retention Rapid Equilibration Column : Fortis HILIC DIOL x2.mm 5μ p/n : FDI-255 Mobile Phase: 99: ACN : H 2 O Flow :.2ml/min Temp : 2 C Wavelength: 254nm. 5-Fluorouracil 2. Uracil.7μ,3μ, 5μ L2 Uracils HILIC DIOL 4% 38m2/g Fortis HILIC DIOL Nucleosides are typically difficult to retain due to the ribose or deoxyribose sugar that forms part of their structure. Fortis HILIC provides a good tool to retain and separate these polar analytes in simple mobile phase conditions. Column : Fortis HILIC 5x4.mm 5μ p/n : FHI-535 Mobile Phase: 95:5 ACN : mm NH 3 OAc Flow : ml/min Temp : 2 C Wavelength: 254nm Nucleosides. Uracil 2. Uridine 3. Cytosine 4. Guanosine Fortis HILIC DIOL FDI-23xx FDI-25xx FDI-27xx - Column Diameter 3. FDI-33xx FDI-35xx FDI-37xx - 4. FDI-53xx FDI-55xx FDI-57xx FDI-59xx Replace xx - for.7µm - 3 for 3µm - 5 for 5µm Fortis Amino Guards Length Column Diameter 2. DCDI-2xxG 4. DCDI-5xxG Fortis HILIC FHI-23xx FHI-25xx FHI-27xx - Column Diameter 3. FHI-33xx FHI-35xx FHI-37xx - 4. FHI-53xx FHI-55xx FHI-57xx FHI-59xx Fortis HILIC Guards Length Column Diameter 2. DCHI-2xxG 4. DCHI-5xxG Replace xx - for.7µm - 3 for 3µm - 5 for 5µm - for µm Tel: Tel:

12 Fortis Amino Column Reproducibility Retention of Polars Alternative Selectivity Highly stable ligand density Rapid Equilibration.7μ 3μ 5μ Amino 5% 2 /g m 8 3 L8 Robust Column Bondings Assured Peak Shapes 2% Lower Asymmetry Specification % Higher Efficiency Each Fortis HPLC column is tested using the industries most rigorous QC test, utilising basic analyte probes as well as neutral efficiency markers ensures that the column reproducibility is first class. Fortis columns are also subject to a 2% lower peak shape specification than other manufacturers columns. QA Test QC Test FNH Mobile Phase: 75 : 25 ACN : H2O Exceptional for Carbohydrate Alternative Selectivity Rapid Equilibration Flow :.ml/min Temp : 3 C Wavelength: RI. Ribose 2. Xylose 4. Glucose. Maltose x4.mm 5μ p/n: F8-555 By employing a QC mix that accurately probes silanol activity (the measure of good peak shape) the analyst can be assured of quality time and time again..ml/min Temp: 25 C Wavelength: 254nm Gains are also made in: 4 5. Sucrose Column: - Sample carry over - Increased Resolution - Increased Sensitivity 7 7. Lactose Phenol Ethylaniline 4. N,N-Dimethylaniline 5. Napthalene... QC HP5 Mobile Phase: :4 ACN:H2O. Uracil 2 3. Fructose Fortis stationary phases have been proven to exhibit excellent peak shapes and efficiency for the full range of analyte species. 22 Volts p/n : Fortis Amino 5x4.mm 5μ Column : Fortis Amino is optimised not only to help retain and resolve hydrogen bonding compounds, but to also be complimentary in resolution to other Fortis phases Carbohydrates Fortis Amino Fortis Amino allows the separation of compounds with reversed phase, normal phase or ion-exchange mechanisms. The Amino bonding is extremely rugged and reproducible to give stable baselines, retention times and selectivity. Amino columns are particularly suited for carbohydrate species. Fortis Amino is now also available in.7µm particle size for UHPLC work. Volts ¾ Colum ¾ p/n : ¾ Mobile ¾ Flow : ¾ Temp : ¾ λ:. Urac 2. Phe 3. 4-Et 4. N,N5. Nap Column Reproducibility Fortis Amino Column Diameter Fortis Amino Guards FNH-23xx FNH-25xx FNH-27xx - 3. FNH-33xx FNH-35xx FNH-37xx - 4. FNH-53xx FNH-55xx FNH-57xx FNH-59xx Replace xx - for.7µm - 3 for 3µm - 5 for 5µm Length Column Diameter 2. DCNH-2xxG 4. DCNH-5xxG Fortis columns are subject to tight specification using basic analytes in an unbuffered mobile phase system. If there were residual uncovered hydroxyl groups present then these basic probes would highlight this fact. Fortis Technologies unique bondings combined with the ultra pure silica matrix ensure that peak shapes and lifetime achieved are first class. Asymmetry Ethylaniline N,N-Dimethylaniline Napthalene Tel: Tel: columns 23

13 Capillaries Prep 5µm and µm particles High Loadability Optimised Packing Efficiency Narrow peak profile, High Efficiency and Resolution Fortis Prep columns are designed for high sample loading, high throughput applications. The optimised packed bed (OPB) process ensures excellent peak shapes and efficiency, whilst the lifetime of the column is increased. Capillaries Fortis capillaries are available in 75µm and 2µm i.d. with any phase chemistry and any particle size from the Fortis range. To find out more about the phase chemistry characteristics please refer to the full Product brochure: Column Diameter µm C75-5-xx-F8 C75--xx-F8 C75-5-xx-F8 C75-25-xx-F8 2µm C2-5-xx-F8 C2--xx-F8 C2-5-xx-F8 C2-25-xx-F8 Fortis Prep Replace xx - for.7µm - 3 for 3µm - 5 for 5µm BSA testing capillary Fortis stationary phases have been proven to provide sharp peaks shapes, critical for high resolution separations in small low dose formats. fmol/microlitre BSA 3µ 75µm x 5mm 4min gradient 3nl/min Improvements are made in: - Reduced sample carry over - Increased Resolution - Increased Sensitivity Relative Abundance Relative Abundance Time (min) Time (min).95 NL: 7.32E9 TIC MS BSAtest_ NL:.38E9 TIC MS BSAtest2_ Columns & Bulk Fortis Prep columns come in sizes from 3mm to 25mm in length and from mm in diameter all the way up to 5mm (2 i.d.). Pre-packed columns are advised for < 2 i.d. after this Bulk material can be supplied for those wishing to pack DAC (Dynamic Axial Compression) columns. If preparative columns are packed with the identical media to their analytical counterpart then the ability to scale up with the theoretical calculations will be accurate. - mm, 2.2mm and 3mm i.d. - 5µm and µm particles - g to multi Kg bulk available Contact us for more information on availability of prep options/bulk packings, or to discuss your application and the ability to scale up. Our technical experts will be happy to discuss your needs with you. Tel: Tel:

14 Filter & Guard Options Guard system for all 3µm, 5µm and µm phases Low volume in-line filters for LC and UHPLC Maintain chromatographic integrity Fortis Guards and filters are designed to ensure that erroneous materials do not find there way onto the more important and expensive analytical column. Guards are available in sizes to match all analytical and preparative column dimensions. Filters are particularly suitable for short fast LC/MS (Pace ) columns and UHPLC columns. Direct connect guard system for all 3µm, 5µm and µm phases Quick replacement cartridges - Highly Cost Effective 5µm Fortis Guard Cartridges DCGUA- Guard Cartridge Holder DCxx-45G/2 x4mm Fortis 5µm Guard pk 2 DCxx-45G/4 x4mm Fortis 5µm Guard pk 4 DCxx-25G/2 x2mm Fortis 5µm Guard pk 2 DCxx-25G/4 x2mm Fortis 5µm Guard pk 4 Replace xx 8 for PH for Fortis Diphenyl HO for Fortis H2o 8 for Fortis C8 CN for Fortis Cyano HI for Fortis HILIC Preparative guard system mm & 2.2mm Quick replacement cartridges - Highly Cost Effective - Reduced volume coupler available In-line Filter for all LC columns Low volume in-line filters Change over time is seconds not minutes 3µm Fortis Guard Cartridges DCGUA- Guard Cartridge Holder DCxx-43G/2 x4mm Fortis 3µm Guard pk 2 DCxx-43G/4 x4mm Fortis 3µm Guard pk 4 DCxx-23G/2 x2mm Fortis 3µm Guard pk 2 DCxx-23G/4 x2mm Fortis 3µm Guard pk 4 Fortis in-line filters are fingertight direct connect design, fitting in between the column and the conventional peek fitting to filter out particulate matter, it contains low dead volume and pressure. In-line filters are ideal for very short fast columns such as Fortis Pace LC/MS columns where extra packed bed from a guard would be detrimental. In-line filters are also available in UHPLC format, capable of withstanding the elevated pressures involved. Part number tables F8-2xx F8-22xx F8-23xx F8-25xx F8-27xx - Column Diameter 3. - F8-32xx F8-33xx F8-35xx F8-37xx F8-52xx F8-53xx F8-55xx F8-57xx F8-59xx Replace xx - for.7µm - 2 for 2.5µm - 3 for 3µm - 5 for 5µm - for µm Fortis Diphenyl FPH-2xx FPH-22xx FPH-23xx FPH-25xx FPH-27xx - Column Diameter 3. - FPH-32xx FPH-33xx FPH-35xx FPH-37xx FPH-52xx FPH-53xx FPH-55xx FPH-57xx FPH-59xx Replace xx - for.7µm - 2 for 2.5µm - 3 for 3µm - 5 for 5µm - for µm Fortis H2o FHO-2xx FHO-22xx FHO-23xx FHO-25xx FHO-27xx - Column Diameter 3. - FHO-32xx FHO-33xx FHO-35xx FHO-37xx FHO-52xx FHO-53xx FHO-55xx FHO-57xx FHO-59xx Replace xx - for.7µm - 3 for 3µm - 5 for 5µm Fortis C F8-2xx F8-22xx F8-23xx F8-25xx F8-27xx - Column Diameter 3. - F8-32xx F8-33xx F8-35xx F8-37xx F8-52xx F8-53xx F8-55xx F8-57xx F8-59xx Replace xx - for.7µm - 3 for 3µm - 5 for 5µm Fortis Cyano FCN-2xx FCN-22xx FCN-23xx FCN-25xx FCN-27xx - Column Diameter 3. - FCN-32xx FCN-33xx FCN-35xx FCN-37xx FCN-52xx FCN-53xx FCN-55xx FCN-57xx FCN-59xx Replace xx - for.7µm - 3 for 3µm - 5 for 5µm Fortis HILIC FHI-2xx FHI-22xx FHI-23xx FHI-25xx FHI-27xx - Column Diameter 3. FHI-32xx FHI-33xx FHI-35xx FHI-37xx - 4. FHI-52xx FHI-53xx FHI-55xx FHI-57xx FHI-59xx Replace xx - for.7µm - 3 for 3µm - 5 for 5µm - for µm Fortis HILIC DIOL FDI-2xx FDI-22xx FDI-23xx FDI-25xx FDI-27xx - Filter for all UHPLC columns No backpressure increase Increase lifetime of UHPLC columns Low volume in-line filter Change over time is seconds not minutes Analytical In-line Filters 2-SAV5 2µm In-line filter pk 5 2-SAV 2µm In-line filter pk UHPLC In-line Filters UHPSAV2 UHPLC In-line filter pk 2 UHPSAV4 UHPLC In-line filter pk 4 Column Diameter 3. FDI-32xx FDI-33xx FDI-35xx FDI-37xx - 4. FDI-52xx FDI-53xx FDI-55xx FDI-57xx FDI-59xx Replace xx - for.7µm - 3 for 3µm - 5 for 5µm - for µm Fortis Amino FNH-2xx FNH-22xx FNH-23xx FNH-25xx FNH-27xx - Column Diameter 3. FNH-32xx FNH-33xx FNH-35xx FNH-37xx - 4. FNH-52xx FNH-53xx FNH-55xx FNH-57xx FNH-59xx Replace xx - for.7µm - 3 for 3µm - 5 for 5µm - for µm Tel: Tel:

15 Applications Compound Use Column,3-Dimethyluric acid a Hydroxyprogesterone Steroid Fortis H2o SpeedCore Diphenyl a-hydroxyprogesterone Steroid Fortis Cyano 7 a Hydroxyprogesterone Steroid Fortis H2o 7-Hydroxyprogesterone Hormone -Hydroxy-midazolam Anxiolytic 2,4-D Herbicide Fortis Cyano 2,4-DB Herbicide Fortis Cyano 2,4-DCP Herbicide 2,4-DP Herbicide Fortis Cyano 2,-Dimethylphenol SpeedCore C8 2,-Dinitrotoluene Explosives Fortis Cyano 2-Hydroxybenzoic acid Positional Isomers Fortis Diphenyl 2-Hydroxyestradiol Positional Isomers Fortis Diphenyl 2-Nitroaniline Explosives Fortis Cyano 3,4-Dimethylphenol 3,5-Dimethylphenol SpeedCore C8 SpeedCore C8 3-hydroxyanabsinthin Sesquiterpene Lactones 3-Hydroxybenzoic acid Positional Isomers Fortis Diphenyl 3-Methoxytyramine catecholamine Fortis HILIC 3-Nitrobenzoic acid 3-Octanon Fragrence 4-Ethylaniline 4-Hydroxybenzoic acid Positional Isomers Fortis Diphenyl 4-Hydroxyestradiol Positional Isomers Fortis Diphenyl 4-Nitroaniline Explosives Fortis Cyano 5-Fluorouracil anticarcinogen SpeedCore HILIC 5-HIAA Catecholamines Fortis H2o -monacetylmorphine Drugs of Abuse 7-Aminoclonazepam Hypnotic 7-Aminoflunitrazepam Benzodiazepines 7-Aminonitrazepam Anxiolytic Absinthin Sesquiterpene Lactones Acetaminophen Flu Relief Acetic acid Ear Infections Adenine Polars Fortis HILIC ALA Amino Acids Aldehydes Aldehydes Aliskiren Renin Inhibitor SpeedCore C8 Terpineol Plant Hormone Alprazolam Anxiolytic Amiloride Diuretic Amitriptyline Antidepressant Amoxicillin Antibiotic Amphetamine Drugs of Abuse Amprenavir HIV Drugs Anabsin Sesquiterpene Lactones Anabsinthin Sesquiterpene Lactones Angiotensin I Peptide FortisBIO C4 Angiotensin II Peptide FortisBIO C8 Apigenin Natural Dyes Apomyoglobin Protein FortisBIO C8 ARG Amino Acids Artemisetin Sesquiterpene Lactones Ascisic acid Plant Hormone Ascorbic acid Vitamins Fortis HILIC ASP Amino Acids Atazanavir HIV Drugs Atenolol Beta Blocker Fortis H2o Atorvastatin Statins Azithromycin Antibiotic Banvel Herbicide Bendroflumethiazide Thiazide Diuretic Fortis H2o Benoquinone acetic acid Fortis H2o Benzene Alkyl Benzenes Benzoylecgonine Drugs of Abuse Benzyladenine Plant Hormone Bromazepam Benzodiazepines Butylbenzene Alkyl Benzenes Caffeine Alkaloid SpeedCore Diphenyl Campher Fragrance candesartan cilexetil Hypertension Casein Protein FortisBIO C8 Casein Tryptic Digest Catechol FortisBIO C8 SpeedCore C8 Cefachlor Antibiotic Cefadroxil Antibiotic Cefalexin Antibiotic Cefradine Antibiotic Chloramphenicol Antibiotic Fortis H2o Cineol Fragrance Ciprofloxacin Antibiotic Fortis Diphenyl Citalopram Antidepressant Clonaepam Hypnotic Clopidogrel Hydrogen SO 4 Antiplatelet Fortis C8 Clozapine Drugs of Abuse CMPP Herbicide Fortis Cyano co-amoxiclav Antibiotic co-codamol Pain Relief Cortisone Anti-Inflammatory CYS-CYS Amino Acids Cytochrome C Protein FortisBIO C8 Cytosine Nuclosides Fortis HILIC Applications D3-Digitoxin Cardiac glycosides Dalbavancin Antibiotic Fortis Diphenyl Demoxepam Benzodiazepines Desmethyldiazepam Anxiolytic Dexamethasone Ear Infections Diamorphine opioid analgesic Fortis H2o Dianette Alkaloid Diazepam Anti Anxiety Diclofenac Sodium Painkiller Tel: Tel: Diethylaniline Digitoxin Cardiac glycosides Dihydroquinidine antiarrhythmic Fortis Diphenyl Diltiazem High Blood Pressure Fortis H2o Dimethylaniline Diphenhydramine Antihistamine d-metafetamine Drugs of Abuse DOPAC Catecholamines Fortis H2o Dopamine catecholamine Fortis HILIC Doxazosin alpha-blocker Fortis Diphenyl Entecavir Antiviral Fortis Diphenyl Epinephrine catecholamine Fortis HILIC Epiyangambin Sesquiterpene Lactones Erythromycin Erythromycin Fortis HILIC Estradiol Valerate SpeedCore Diphenyl Estradiols Estradiols Fenuron Flucloxacillin Antibiotic Flunitrazepam Anxiolytic Fluoruracil Polars Fortis HILIC Fluoxetine Antidepressant Folic Acid Vitamin Fortis H2o Fructose monosaccharide Fortis Amino Gabapentin Epilepsy Gibberellin acid Plant Hormone Gliclazide Diabetes GLU Amino Acids Glucose monosaccharide Fortis Amino GLY Amino Acids GLY-TYR Peptide FortisBIO C8 Guanosine Nuclosides Fortis HILIC Haloperidol Antipsychotic Heptylbenzene Alkyl Benzenes Hexylbenzene Alkyl Benzenes HIS Amino Acids Holo-Transferrin Protein FortisBIO C8 Homogentisic acid Fortis H2o Human Growth Hormone Peptide Hormone FortisBIO C8 Hydroxy-2-acetate Steroid Fortis Cyano Hydroxyphenylacetic acid Fortis H2o Hydroxyphenylpyruvic acid Fortis H2o Hydroxytisone-2-acetate Steroid Fortis H2o Ibuprofen Painkiller ILE Amino Acids Indol-3-yl-acetate Plant Hormone Insulin Peptide FortisBIO C4 Irbesartan Angiotensin II antagonist Isoascorbic acid Vitamins Fortis HILIC Isonicatinamide Positional Isomers Fortis Diphenyl Ketopelenolide Sesquiterpene Lactones Kinetin Plant Hormone Lactose disaccharide Fortis Amino Lamotrigine Epilepsy Fortis C8 Lanalool Fragrance Lanandulyl acetate Lansoprazole Stomach Ulcers Lavandulol LEU Amino Acids LEU-Enkephalin Peptide FortisBIO C8 Levocetirizine Antihistamine Fortis H2o Lidocaine Irregular Heartbeats Limonen Fragrance Linalyl Acetate Fragrance Lopinavir HIV Drugs Loratadine Antihistamine Lorazepam Anti Anxiety Fortis Diphenyl LSD Drugs of Abuse Luteolin Natural Dyes LYS Amino Acids Maltose disaccharide Fortis Amino MCPA Agrochemicals MCPB Weed Control Fortis Cyano m-cresol SpeedCore C8 MDA Drugs of Abuse MDEA Drugs of Abuse MDMA (Ecstasy) Drugs of Abuse Melamine Fortis HILIC MET Amino Acids Metanephrine catecholamine Fortis HILIC MET-Enkephalin Peptide FortisBIO C8 Methamphetamine Drugs of Abuse Methyl Melonic acid Organic acids Fortis H2o Methylbenzoate Midazolam Anxiolytic Mirtazapine Antidepressant Morphine Drugs of Abuse N,N-Dimethylaniline QC Test Naphthalene QC Test Nelfinavir HIV Drugs

16 Applications Neomycin Sulphate Ear Infections Nicotinamide Positional Isomers Fortis Diphenyl Nicotinic acid Vitamins Fortis HILIC Nitosininone Fortis H2o Nitrazepam Anxiolytic Nitrobenzene Explosives Fortis Cyano Nogestrel SpeedCore Diphenyl Nordiazepam Drugs of Abuse Normetanephrine catecholamine Fortis HILIC Norpinephrine catecholamine Fortis HILIC Nortriptyline Tricyclic Antidepressants o-cresol SpeedCore C8 -Dalbavancin Antibiotic Fortis Diphenyl Olanzapine antipsychotic SpeedCore C8 Omeprazole Stomach Ulcers Oseltamivir Antiviral Oxazepam Hypnotic PAH PAH EPA Paracetamol Flu Relief Paroxetine Antidepressant Fortis H2o PCOC Weed Control Fortis Cyano p-cresol SpeedCore C8 Pentylbenzene Alkyl Benzenes Pesticides KFDA83-59 Pesticides PHE Amino Acids Phenol SpeedCore C8 Phenoxymethylpenicillin Antibiotic Phenylephrine Flu Relief Pheophorbide Pheophytin PK95 PET Tracer Fortis Diphenyl PK95 Dechlorinated PET Tracer Fortis Diphenyl Prednisolone Steroid Fortis H2o Prednisone Steroid Fortis H2o Premarin Estrogens SpeedCore HILIC PRO Amino Acids Procaine Anestetic Prochlorperazine Maleate phenothiazine antipsychotics Fortis Diphenyl Progesterone Steroid Fortis H2o Proguanil Anti Malarial Fortis Diphenyl Promethazine theoclate Nausea Propylbenzene Alkyl Benzenes Protriptyline Antidepressant Pyrazoline Pyridine Pyridoxine Polars Fortis HILIC Pyropheophytin Quetiapine antipsychotic SpeedCore C8 Quinidine antiarrhythmic Fortis Diphenyl Raloxifene Glucoronides treat osteoporosis Rasagiline Parkinsons SpeedCore C8 Resorcinol SpeedCore C8 Riboflavin Vitamins Fortis HILIC Ribonuclease A Protein FortisBIO C8 Ribose monosaccharide Fortis Amino Ritonavir HIV Drugs Rosuvastatin Statins Sequinavir HIV Drugs SER Amino Acids Serotonin Catecholamines Fortis H2o Sesartemin Sesquiterpene Lactones Simvastatin High Blood Pressure Fortis H2o Sotalol Beta Blocker Succinic acid Organic acids Fortis H2o Sucrose disaccharide Fortis Amino Sulfamerazine Sulfa Drugs Sulfamethoxazole Sulfa Drugs Sulfathiazole Sulfa Drugs Sumatriptan UniverSil HS C8 Telmisartan Hypertension Fortis C8 Temazepam Anti Anxiety Tenofovir HIV Drugs Fortis H2o Terpinen 4 ol Fragrance Testosterone Hormone Theobromine SpeedCore Diphenyl Theophylline Alkaloid THR Amino Acids Thymidine (IS) HIV Drugs Fortis H2o Tiotropium bromide bronchodilator Toluene Polars Fortis HILIC Tramadol Opioid Painkiller Trimipramine Antidepressant TYR Amino Acids Tyrosine Amino Acids Fortis H2o Uracil Nuclosides Fortis HILIC Uridine Nuclosides Fortis HILIC VAL Amino Acids Valproate Semisodium Manic Depression Fortis C8 VAL-TYR-VAL Peptide FortisBIO C8 Verapamil Irregular Heartbeats Vitamin C Vitamins Fortis HILIC Warfarin anticoagulant Fortis H2o Xylose monosaccharide Fortis Amino Zolpidem Hypnotic Zopiclone Hypnotic Fortis Phase Characteristics Particle Size Surface Area (m 2 /g) %C Pore Size ph range USP.7µ 3µ 5µ µ L Fortis H2o.7µ 3µ 5µ L Fortis Diphenyl.7µ 3µ 5µ µ L Fortis C8.7µ 3µ 5µ µ L7 Fortis HILIC.7µ 3µ 5µ µ 38 N/A 2-8 L3 Fortis HILIC Diol.7µ 3µ 5µ L2 Fortis Cyano.7µ 3µ 5µ L Fortis Amino.7µ 3µ 5µ L8 Particle Size Particle Size Surface Area (m 2 /g) Surface Area (m 2 /g) %C Pore Size ph range USP FortisBIO C8.7µ 5µ L FortisBIO C4.7µ 5µ L2 %C Pore Size ph range USP SpeedCore C8 2.µ L SpeedCore Diphenyl 2.µ L SpeedCore PFP (PentaFluoroPhenyl) 2.µ L43 SpeedCore HILIC 2.µ 4 N/A L3 Tel: Tel:

17 WORLDWIDE AVAILABILITY Fortis products are available worldwide. For the distributor in your country, contact Fortis international Sales Office, UK by telephone, fax or 45 Coalbrookdale Road Clayhill Industrial Park Neston Cheshire, UK CH4 3UG t: f: e: info@fortis-technologies.com Austria Bangladesh Brazil Canada China Columbia Czech Republic Ecuador Egypt France Germany Greece Holland Hong Kong Hungary India Ireland Israel Italy Japan Korea Malaysia Mexico Netherlands Norway Puerto Rico Poland Portugal Romania Russia Singapore South Africa Spain Sweden Switzerland Taiwan Thailand Turkey USA For technical support or applications contact : technicalsupport@fortis-technologies.com For more information VISIT : Fortis Diphenyl, Fortis H2o, Fortis C8, Fortis Cyano, Fortis HILIC, Fortis Pace are registered trademarks of Technologies Ltd. Sunfire, Xbridge, Acquity BEH, UPLC, Atlantis and Xterra are registered trademarks of Waters Corporation. Luna, Gemini,Kinetex and Synergi are registered trademarks of Phenomenex. Zorbax Eclipse XDB and Zorbax SB-C8 are registered trademarks of Agilent Technologies. Inertsil is a registered trademark of GL Sciences. ACE is a registered trademark of ACT Fortis is not associated with these companies. Comparative separations may not be representative of all applications. All columns are original manufacturers own. 24 Fortis Technologies Ltd. All rights reserved.