1.7µm Fortis C18 UHPLC Columns

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1 Strength in Technology.7µm Fortis C8 Columns Ultra High Pressure Chromatography Increase Efficiency Increase Speed Improve Resolution Greater Sensitivity Wider Linear flow rate range

2 .7µm Columns - 8m/g Surface Area Provides Increased Peak Capacity High Efficiency small particles Robust Reproducible columns Operate to 8,psi Fully Scalable to Analytical and Prep Size.7µm Fortis particles are designed to provide characteristics, which will aid in increased productivity within ultra high pressure chromatography (). Designed to be robust, reproducible and fully scalable with µm, 5µm and µm particles..7µm Fortis particles will operate upto bar providing high linear velocities, increased efficiency, and allowing speed and sensitivity to be achieved on all the latest systems. By choosing a high surface area (S.A.) phase the analyst can increase peak capacity using their existing column dimension, or maintain existing capacity whilst lowering backpressure on a shorter column. Increased Efficiency.7µm Fortis C8 provides increased efficiency over µm and 5µm particles. This gives the opportunity to increase resolution or speed of analysis. A wider linear flow range means that more resolution and peak capacity can be achieved for a given separation. By having a consistent particle size,.7µm Fortis C8 ensures pressure is reduced whilst no loss in efficiency is seen. -Ethylaniline,-Dimethylaniline -Ethylaniline apthalene.7µm Fortis C8 x.mm bar Acquity BEH C8.7µm 5x.mm Fully Scaleable Critically important to the analyst is the ability to have a fully scalable separation. Fortis C8 can be scaled from.7µm all the way through analytical and 5µm particles to prep size without any change in retention profile. By combining the same surface area, pore size characteristics with the identical bonding the analyst can be ensured of having the ability to either scale up methods to traditional LC systems, or to be confident that a method can be transferred to another laboratory with the same selectivity being achieved. If a small particle used in is not the same as its larger um and 5um particle then changes in resolution and retention can occur, both of which can cause problems in method validity..7µm Fortis C8 will alleviate all these potential issues, leaving the analyst confident in method transfer..7µm Fortis C8 = 9,67 =,9 5µm Fortis C8 = 88, HPLC_Acquity_nov_ AU e- 5.e-.5e-. Competitor.7µm Hybrid C issue? : Diode Array Range:.5 issue? Higher Efficiency Greater Resolution Increased Sensitivity - Fully Scalable 8m /g S.A.,-Dimethylaniline apthalene 5 bar AU -.5e- -5.e HPLC_X Brige_nov_ : Diode Array.9 Range: e- 5.e-.5e- Competitor.5µm Hybrid C Comparison of Hydrophobicity and Peak Shape 85m /g m /g.7µm Fortis C8 5x.mm Surface Area 8m /g Efficiency 9,67 Peak Shape (,-Dimethylaniline) Psi -.ml/min (6: AC:Water) Psi -.ml/min (6: AC:Water). 7bar 5bar Acquity BEH.7µm C8 5x.mm Surface Area 85m /g Efficiency 67, Sensitivity Gains Critically peak height increases in mode due to the rise in efficiency () from the smaller particle, but also it is also inversely proportional to peak width, so symmetrical peaks will lead to increased sensitivity. By moving from a particle size to.7µm Fortis C8 sensitivity can be increased. In this example peak height goes up by over 7%. The increase from 5um particles is even greater.. -.5e- -5.e-.7 Major Pharmaceutical Company Data Time Peak Shape (,-Dimethylaniline).8 Psi -.ml/min (6: AC:Water) Psi -.ml/min (6: AC:Water) bar 9bar All columns 5x.mm

3 µm Fortis C8 ph options ph selectivity for method development ph stable - Gives high speed of equilibration.7µm Fortis C8 will operate across the ph spectrum giving the analyst the ability to optimise the correct ph region for their separation. Quickly equilibrating from formic acid to ammonium acetate through to ammonia allows ph, as a method variable, to be rapidly evaluated. Resolution of compounds can be changed radically by altering ph to optimise separation between compound classes. Column:.7μm Fortis C8 x.mm p/n: F8- Gradient: - 5% in 5min.ml/min Temp: C Wavelength: 5nm ph.. Uracil. Procaine. Fenuron. -itrobenzoic acid Phase Chemistry Resolution vs Efficiency vs.7µm Fortis C8 will provide Hydrophobic selectivity which is suitable for many compounds. However as we can see from the resolution equation having multiple phase chemistries available is a definite advantage. can then be used in conjunction with efficiency of the small particle..7µm Fortis columns are also available as:.7µm Fortis Diphenyl.7µm Fortis Cyano.7µm Fortis HILIC Efficiency R= = k k + k k - () has the greatest impact on improving resolution. Resolution (R) k k Improve ph 7. If we are scaling a method and hoping that an increase in efficiency alone will provide the necessary resolution we can be disappointed. Scaling from µm to.7µm C8 has not provided baseline resolution between the compounds. Adding selectivity by phase chemistry into the equation has allowed us to go faster on a shorter column and now achieve full separation. In this instance.7µm Fortis Diphenyl provides more resolution than C8. This then leads to the ability to increase speed by use of shorter columns, higher temperature, change in organic or a combination of the above. Efficiency Alone 5x.mm.7µm Fortis C8 5x.mm Efficiency +.7µm Fortis Diphenyl x.mm ph. mins Isonicotinamide and icotinamide.5mins

4 Method Development Transfer from HPLC to Optimising resolution is one of the main reasons that we wish to move from HPLC to, along with increased speed. If we can change the hydrophobicity of the phase chemistry then we have the potential to reduce the retention time. If we can then move to a smaller particle and increase resolution between our critical pairs we can improve the speed of analysis still further. x.mm µm Fortis C8 x.mm Column: Fortis C8 x.mm μ Mobile Phase: 6/ AC/mM Phosphate ph 7..ml/min Temp: 5 C Wavelength: 5nm = Less. Doxepin. Imipramine. ortriptyline 5. Amitriptyline Sample Filter Filter for all columns o backpressure increase Increase lifetime of columns Low volume in-line filter Change over time is seconds not minutes Fortis in-line filters are direct connect design, fitting in between the column and the conventional fitting to filter out particulate matter. They contain low dead volume and pressure. In-line filters are ideal for.7µm Fortis columns where extra packed bed from a guard would be detrimental. in-line filters are manufactured to withstand,psi..7µm part numbers Efficiency = More Resolution.7um Fortis C8 F8-7.7µm Fortis C8 5x.mm FPH-7.7um Fortis Diphenyl.7µm Fortis Diphenyl 5x.mm.7µm Fortis C8 F8-5.7µm Fortis C8 x.mm FPH-5.7µm Fortis Diphenyl x.mm x.mm F8-.7µm Fortis C8 5x.mm FPH-.7µm Fortis Diphenyl 5x.mm Column:.7μm Fortis C8 x.mm F8-.7µm Fortis C8 x.mm FPH-.7µm Fortis Diphenyl x.mm Mobile Phase: 6/ AC/mM Phosphate ph 7. F8-.7µm Fortis C8 x.mm FPH-.7µm Fortis Diphenyl x.mm.5mins.ml/min Temp: C Wavelength: 5nm F8-5.7µm Fortis C8 x.mm F8-.7µm Fortis C8 5x.mm F8-.7µm Fortis C8 x.mm FPH-5 FPH- FPH-.7µm Fortis Diphenyl x.mm.7µm Fortis Diphenyl 5x.mm.7µm Fortis Diphenyl x.mm F8-5.7µm Fortis C8 5x.6mm FPH-5.7µm Fortis Diphenyl 5x.6mm Transfer from HPLC to.7um Fortis Cyano.7um Fortis HILIC FC-7.7µm Fortis Cyano 5x.mm FHI-7.7µm Fortis HILIC 5x.mm Greater speed can be achieved by moving to smaller particles, whilst still obtaining full baseline resolution. FC-5 FC- FC-.7µm Fortis Cyano x.mm.7µm Fortis Cyano 5x.mm.7µm Fortis Cyano x.mm FHI-5 FHI- FHI-.7µm Fortis HILIC x.mm.7µm Fortis HILIC 5x.mm.7µm Fortis HILIC x.mm.7µm Fortis C8 will operate at higher linear flow rates whilst providing lower backpressure than other sub µm particles. x.mm.7µm.9ml/min x.mm.7µm.ml/min FC- FC-5 FC- FC-.7µm Fortis Cyano x.mm.7µm Fortis Cyano x.mm.7µm Fortis Cyano 5x.mm.7µm Fortis Cyano x.mm FHI- FHI-5 FHI- FHI-.7µm Fortis HILIC x.mm.7µm Fortis HILIC x.mm.7µm Fortis HILIC 5x.mm.7µm Fortis HILIC x.mm FC-5.7µm Fortis Cyano 5x.6mm FHI-5.7µm Fortis HILIC 5x.6mm To move to smaller particles and smaller columns, without affecting selectivity, use some simple scaling equations to optimise your parameters: us for the spreadsheet: technicalsupport@fortis-technologies.com x.6mm 5µm ml/min

5 WORLDWIDE AVAILABILITY Fortis products are available worldwide. For the distributor in your country, contact Fortis international Sales Office, UK by telephone, fax or 5 Coalbrookdale Road Clayhill Industrial Park eston Cheshire, UK CH6 UG t: f: e: info@fortis-technologies.com Austria Bangladesh Brazil Canada China Columbia Czech Republic Ecuador Egypt France Germany Greece Holland Hong Kong Hungary India Ireland Israel Italy Japan Korea Malaysia Mexico etherlands orway Puerto Rico Poland Portugal Romania Russia Singapore South Africa Spain Sweden Switzerland Taiwan Thailand Turkey USA For technical support or applications contact : technicalsupport@fortis-technologies.com For more information VISIT : Fortis C8, Fortis Diphenyl, Fortis Ho, Fortis C8, Fortis Cyano, Fortis HILIC, Fortis Pace are trademarks of Fortis Technologies Ltd. Sunfire, Xbridge, Acquity BEH, UPLC, Atlantis and Xterra are registered trademarks of Waters Corporation. Luna, Gemini,Kinetix and Synergi are registered trademarks of Phenomenex. Zorbax Eclipse XDB and Zorbax SB-C8 are registered trademarks of Agilent Technologies. Inertsil is a registered trademark of GL Sciences. ACE is a registered trademark of ACT Fortis is not associated with these companies. Comparative separations may not be representative of all applications. All columns are original manufacturers own. - Fortis Technologies Ltd. All rights reserved.