SUPPLEMENTARY MATERIAL. The table lists the 437 proteins proposed to be myristoylated in A. thaliana. The AGI

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1 SUPPLEMENTARY MATERIAL Table s1: the N-myristoylome in A. thaliana The table lists the 437 proteins proposed to be myristoylated in A. thaliana. The AGI entries were re-annotated according to the most recent reviews and nomenclature proposals (36,39-41,70). Assay means that the data in available in Table1, 2 or 3. Score was obtained as described in the experimental procedures with the Arabidopsis Predictor tool. nd, not determined. CC/ST is cell communication and signal tranduction; CR, cell, rescue; M, metabolism; NYC, not yet clear (often development) PD, protein destination; PS, protein synthesis; T, transcription; TF, transport facilitation; U, unknown. Class AGI entry Sequence Function Assay CC/ST At5g24270 MGCSVSKKKK Calcium sensor homolog SOS CC/ST At5g47100 MGCFHSTAAR Calcium sensor protein. calcineurin-like nd 6.9 CC/ST At5g08160 MGCSFSGLNA Protein kinase (serine/threonine) nd 4.7 CC/ST At1g26970 MGNCFGFSAK Protein kinase (serine/threonine) homolog nd 7.9 CC/ST At3g17410 MGCFGCCGGG Protein kinase (serine/threonine) homolog nd 0.1 CC/ST At5g15080 MGLDAVKAKG Protein Kinase (serine/threonine) like nd 3.6 CC/ST At3g08870 MGIARSINSF Protein kinase (serine/threonine) putative nd 1.6 CC/ST At5g02290 MGGCFSNRIK Protein kinase (Serine/Threonine) Specific NAK nd 4.8 CC/ST At5g21170 MGNANGKDED Protein Kinase AKIN beta CC/ST At4g16360 MGNVNAREEA Protein Kinase AKIN beta2 nd 10.3 CC/ST At1g07570 MGICLSAQVK Protein Kinase APK1A nd 2.7 CC/ST At2g28930 MGICLSAQIK Protein Kinase APK1B nd 1.6 CC/ST At4g26470 MGGAVTKSET Protein kinase calcium dependant probable nd 4.3 CC/ST At5g57630 MGLFGTKKIG Protein Kinase CIPK21 (SnRK3.4) nd 5.3 CC/ST At5g04870 MGNTCVGPSR Protein Kinase CPK1 nd 3.4 CC/ST At3g10660 MGNACVGPNI Protein Kinase CPK CC/ST At4g23650 MGHRHSKSKS Protein Kinase CPK CC/ST At4g35310 MGNSCRGSFK Protein Kinase CPK5 nd 0.5 CC/ST At2g17290 MGNSCRGSFK Protein Kinase CPK6 nd 0.3 CC/ST At5g12480 MGNCCGNPSS Protein Kinase CPK7 nd 7.2 Sco -re

2 CC/ST At5g19450 MGNCCASPGS Protein Kinase CPK8 nd 5.9 CC/ST At3g20410 MGNCFAKNHG Protein Kinase CPK9 nd 14.7 CC/ST At1g18890 MGNCNACVRP Protein Kinase CPK10 nd 2.9 CC/ST At3g51850 MGNCCRSPAA Protein Kinase CPK13 nd 0.2 CC/ST At2g41860 MGNCCGTAGS Protein Kinase CPK14 nd 7.6 CC/ST At4g21940 MGCFSSKHRN Protein Kinase CPK15 nd 11.1 CC/ST At2g17890 MGLCFSSAAK Protein Kinase CPK16 nd 5.0 CC/ST At5g12180 MGNCCSHGRD Protein Kinase CPK17 nd 11.9 CC/ST At4g36070 MGLCFSSPKA Protein Kinase CPK18 nd 4.4 CC/ST At2g38910 MGNTCVGPNL Protein Kinase CPK20 nd 2.5 CC/ST At4g04720 MGCFSSKHRK Protein Kinase CPK21 nd 11.6 CC/ST At4g04710 MGNCCGSKPL Protein Kinase CPK22 nd 2.9 CC/ST At4g04740 MGCFSSKHRK Protein Kinase CPK23 nd 11.6 CC/ST At2g31500 MGSCVSSPLK Protein Kinase CPK24 nd 2.2 CC/ST At2g35890 MGNVCVHMVN Protein Kinase CPK25 nd 6.9 CC/ST At4g04700 MGCFSSKELQ Protein Kinase CPK27 nd 11.8 CC/ST At1g74740 MGNCIACVKF Protein Kinase CPK30 nd 1.5 CC/ST At4g04695 MGCYSSKNLK Protein Kinase CPK31 nd 11.8 CC/ST At3g57530 MGNCCGTAGS Protein Kinase CPK32 nd 7.2 CC/ST At1g50700 MGNCLAKKYG Protein Kinase CPK33 nd 12.1 CC/ST At5g19360 MGNCCSHGRD Protein Kinase CPK34 nd 13.4 CC/ST At2g41140 MGICHGKPVE Protein Kinase CRK1 nd 4.1 CC/ST At3g19100 MGGCTSKPSS Protein Kinase CRK2 nd 8.8 CC/ST At2g46700 MGQCYGKVNQ Protein Kinase CRK3 nd 7.6 CC/ST At5g24430 MGHCYSRNIS Protein Kinase CRK CC/ST At3g50530 MGLCTSKPNS Protein Kinase CRK5 nd 9.8 CC/ST At3g49370 MGHCYSRNIS Protein Kinase CRK CC/ST At3g56760 MGLCHGKPIE Protein Kinase CRK7 nd 3.8 CC/ST At1g49580 MGGCTSKPST Protein Kinase CRK8 nd 8.3 CC/ST At5g44290 MGCIISKKKS Protein kinase putative nd 13.1 CC/ST At4g15650 MGCGKSKHDV Protein kinase putative nd 12.9 CC/ST At5g41260 MGCEVSKLSA Protein kinase putative nd 12.8 CC/ST At4g10010 MGCICSKGTA Protein kinase putative nd 12.8 CC/ST At1g33770 MGCICSKGAA Protein kinase putative nd 12.6 CC/ST At5g56460 MGNCWCRFEP Protein kinase putative nd 12.3 CC/ST At5g39420 MGCISSKNVS Protein kinase putative nd 12.0 CC/ST At1g18670 MGCVNSKQTV Protein kinase putative nd 11.6 CC/ST At5g01020 MGNCGTRDEA Protein kinase putative nd 10.8 CC/ST At1g01740 MGGQSSKIGT Protein kinase putative nd 10.7 CC/ST At5g46570 MGCLHSKTAN Protein kinase putative nd 10.5 CC/ST At5g50860 MGCVLCKEST Protein kinase putative CC/ST At1g09600 MGCNCTKGTR Protein kinase putative nd 10.0 CC/ST At1g57700 MGCICSKGVR Protein kinase putative nd 10.0 CC/ST At5g59010 MGPRCSKLSL Protein kinase putative nd 9.7 CC/ST At3g54030 MGARCSKFSF Protein kinase putative nd 9.6 CC/ST At1g03740 MGCVNSRHRP Protein kinase putative nd 9.0

3 CC/ST At1g74330 MGCVSSKQTV Protein kinase putative nd 8.7 CC/ST At5g25440 MGNCLSASTL Protein kinase putative nd 8.2 CC/ST At1g54610 MGCVFGREAA Protein kinase putative nd 7.3 CC/ST At3g05050 MGCVLGRPGS Protein kinase putative nd 7.0 CC/ST At4g22940 MGCIISYRRN Protein kinase putative nd 6.1 CC/ST At2g17090 MGCCYSLSST Protein kinase putative nd 5.5 CC/ST At2g39660 MGSCFSSRVK Protein kinase putative nd 5.4 CC/ST At5g03640 MGSFAGKCEI Protein kinase putative nd 5.4 CC/ST At1g53050 MGCVCGKPSA Protein kinase putative nd 5.2 CC/ST At4g35600 MGACISFFSS Protein kinase putative nd 4.9 CC/ST At1g71530 MGCICATARS Protein kinase putative nd 3.8 CC/ST At2g07180 MGICFSAEDQ Protein kinase putative nd 3.8 CC/ST At1g07870 MGCFGCSKKS Protein kinase putative nd 0.4 CC/ST At1g50990 MGCCQSSFLK Protein kinase putative (PPC:1.16.1) nd 6.1 CC/ST At5g13160 MGCFSCFDSS Protein kinase putative PBS1 resistance nd 0.2 CC/ST At4g20940 MGQLPSQDIM Protein kinase receptor putative nd 5.7 CC/ST At2g19230 MGNFNFLPLV Protein kinase receptor putative nd 4.9 CC/ST At5g01540 MGTQRSMFIV Protein kinase receptor putative nd 0.8 CC/ST At5g01060 MGCICFKSWR protein kinase receptor putative TMK1 nd 8.3 CC/ST At1g60490 MGANEFRFFL Phosphatidylinositol 3-kinase (AtVps34) nd 2.0 CC/ST At5g39400 MGLKLSRGPV Protein phosphatase PTEN CC/ST At1g03590 MGGCISKTSW Protein phosphatase putative nd 7.9 CC/ST At4g01420 MGCVCSKQLE Protein phosphatase putative nd 10.2 CC/ST At4g17615 MGCFHSKAAK Protein phosphatase putative calcineurin B-like nd 11.9 CC/ST At1g16220 MGLCHSKIDK Protein phosphatase-2c (PP2C) homolog nd 9.0 CC/ST At1g07630 MGNGVTKLSI Protein phosphatase-2c (PP2C) homolog nd 13.6 CC/ST At3g06270 MGCVQCKCCS Protein phosphatase-2c (PP2C) homolog nd 9.9 CC/ST At2g20050 MGCAYSKTCI Protein phosphatase-2c (PP2C) homolog nd 9.7 CC/ST At5g02400 MGNGVTTLTG Protein phosphatase-2c (PP2C) homolog nd 7.9 CC/ST At5g26010 MGHCFSLPSS Protein phosphatase-2c (PP2C) homolog nd 5.6 CC/ST At1g79630 MGLCYSVDRT Protein phosphatase-2c (PP2C) homolog nd 4.8 CC/ST At4g31860 MGIYLSTPKT Protein phosphatase-2c (PP2C) homolog nd 4.7 CC/ST At5g36250 MGSCLSSSGG Protein phosphatase-2c (PP2C) homolog nd 3.9 CC/ST At2g25070 MGTYLSSPKT Protein phosphatase-2c (PP2C) homolog nd 2.0 CC/ST At3g02750 MGSCLSAESR Protein phosphatase-2c (PP2C) homolog nd 3.2 CC/ST At2g26300 MGLLCSRSRH GTP-binding protein GPA1 (alpha subunit) CC/ST At1g23490 MGLSFAKLFS Small G protein ARFA1a CC/ST At5g14670 MGLNFTKLFS Small G protein ARFA1b CC/ST At2g47170 MGLSFGKLFS Small G protein ARFA1c CC/ST At1g70490 MGLSFAKLFS Small G protein ARFA1d CC/ST At3g62290 MGLSFGKLFS Small G protein ARFA1e CC/ST At1g10630 MGLSFAKLFS Small G protein ARFA1f CC/ST At2g15310 MGARFSRIAK Small G protein ARFB1a CC/ST At5g17060 MGQAFRKLFD Small G protein ARFB1b CC/ST At3g03120 MGQTFRKLFD Small G protein ARFB1c CC/ST At3g22950 MGAFMSRFWF Small G protein ARFC

4 CC/ST At1g02440 MGTTLGKPFA Small G protein ARFD1a CC/ST At1g02430 MGTALGKPFA Small G protein ARFD1b nd 6.3 CC/ST At2g24765 MGILFTRMFS Small G protein ARFE CC/ST At3g54840 MGCASSLPDR Small G protein RABF1 (ARA6) CR At3g44670 MGSVMSLGCS NBS/LRR disease resistance protein RPP CR At3g44630 MGSAMSLSCS NBS/LRR disease resistance protein (RPP1-like) nd 6.6 CR At3g44480 MGSVMSLGCS NBS/LRR disease resistance protein (RPP1-like) CR At5g47260 MGNNFSVESP NBS/LRR disease resistance protein (RPS2-like) nd 14.0 CR At5g05400 MGACFSVAIS NBS/LRR disease resistance protein (RPS2-like) nd 8.5 CR At1g61180 MGSCFSLQVS NBS/LRR disease resistance protein (RPS2-like) nd 7.8 CR At1g15890 MGNCVALEIS NBS/LRR disease resistance protein (RPS2-like) nd 6.2 CR At1g61310 MGSCFSFQIA NBS/LRR disease resistance protein (RPS2-like) nd 4.5 CR At1g12220 MGGCFSVSLP NBS/LRR disease resistance protein RPS CR At1g61300 MGCCFSVQFS NBS/LRR disease resistance protein (RPS5-like) nd 9.6 CR At1g62630 MGISFSIPFD NBS/LRR disease resistance protein (RPS5-like) CR At1g63350 MGISFSIPFD NBS/LRR disease resistance protein (RPS5-like) CR At1g63360 MGISFSIPFD NBS/LRR disease resistance protein (RPS5-like) CR At1g61190 MGNFVCIEIS NBS/LRR disease resistance protein (RPS5-like) nd 7.8 CR At4g14610 MGGCISVSVS NBS/LRR disease resistance protein (RPS5-like) CR At4g10780 MGSCISLQIS NBS/LRR disease resistance protein (RPS5-like) nd 5.0 CR At1g12210 MGGCVSVSLS NBS/LRR disease resistance protein (RPS5-like) CR At5g63020 MGGCVSVSIS NBS/LRR disease resistance protein (RPS5-like) CR At1g12290 MGGCVSVQVS NBS/LRR disease resistance protein (RPS5-like) CR At1g12280 MGACLTLSFS NBS/LRR disease resistance protein (RPS5-like) nd 2.8 CR At5g62740 MGNLFCCVQV Hypersensitive-induced response protein nd 5.2 CR At5g64120 MGLVRSLCLL Peroxidase nd 2.1 CR At5g19880 MGLSKTIPLV Peroxidase nd 1.5 CR At5g39580 MGLVRSFALV Peroxidase ATP24a nd 0.9 CR At5g39950 MGGALSTVFG Thioredoxin h2 (cytosolic) CR At2g40790 MGNHCTRIPC Thioredoxin h6 (cytosolic) nd 11.0 CR At1g59730 MGSNVSSVHD Thioredoxin h7 (cytosolic) nd 1.9 CR At1g69880 MGANVSTPDQ Thioredoxin h8 (cytosolic) nd 6.2 CR At3g08710 MGSCVSKGKG Thioredoxin h9 (cytosolic) nd 8.4 CR At2g48150 MGASASVPER Glutathione peroxidase probable nd 4.6 CR At3g63080 MGASSSSSVS Glutathione peroxidase probable nd 1.0 M At5g27380 MGSGCSSLSY Glutathione synthetase nd 0.2 M At2g47730 MGAIQARLPL Glutathione-S-transferase (GST6) nd 4.4 M At1g74380 MGQDGSPAHK a-galactosyltransferase putative nd 2.5 M At4g22710 MGTASSKSNI Cytochrome P450 homolog nd 6.4 M At2g41510 MGLTSSLRFH Cytokinin oxidase putative nd 4.0 M AT5G54060 MGASSSKRPF Flavonol 3-O-glucosyltransferase-like nd 7.0 M At1g07110 MGSGASKNTE Fructose-6-phosphate 2-kinase/ fructose-2.6-bisphosphatase M At2g04020 MGLPSSLESY GDSL-motif lipase/hydrolase probable nd 1.2 M At5g25880 MGTNQTQISD Similar to malate dehydrogenase nd 0.4 M At1g53580 MGSSSSFSSS Glyoxalase II putative nd 1.3 M At3g53180 MGMTSFTDGP nodulin/glutamate-ammonia ligase - like protein nd 0.0

5 M At3g09540 MGNLHGIHRS Pectate lyase putative nd 5.5 M At2g21610 MGLYKTKSKR Pectinesterase probable nd 5.1 M At5g57190 MGNGNSREAK Phosphatidylserine decarboxylase nd 15.9 M At4g25970 MGNGNSTETK Phosphatidylserine decarboxylase probable nd 11.3 M At1g24430 MGLEITVTSQ Similar to acetyl-coa:benzylalcoholo acetyltransferase nd 0.8 M At4g34650 MGSLSTILRH Squalene synthase putative nd 0.3 M At4g27570 MGGLKFHVLM UDP rhamnose-anthocyanidin-3-glucoside rhamnosyltransferas - nd 0.9 like protein M At4g27560 MGGLKFHVLM UDP rhamnose-anthocyanidin-3-glucoside rhamnosyltransferase - nd 0.9 like protein M At4g14130 MGPSSSLTTI Xyloglucan endotransglycosylase-related protein nd 0.9 NYC At5g63030 MGSMFSGNRM Glutaredoxin-like protein nd 2.8 NYC At1g64500 MGCTSSKQAK Glutaredoxin-like protein nd 8.8 NYC At5g36270 MGIEVCVKAA GSH-dependent dehydroascorbate reductase 1-like nd 2.0 NYC At1g33880 MGTSVSKPVS AIG1-like protein nd 7.7 NYC At3g29770 MGNLCSLFTP Alpha/beta hydrolase putative nd 10.3 NYC At3g19240 MGTSQSREDR DEM 2 protein NYC At4g33400 MGASHSHEDL DEM like protein NYC At5g19110 MGSSLTRLLV Dermal glycoprotein - like nd 6.1 NYC At5g23980 MGNMRSLVKT FRO2 homolog nd 7.4 NYC At2g41280 MGNLMSLVLV Late embryogenesis abundant M10 protein nd 10.2 NYC At2g41270 MGNLKSLVLL Late embryogenesis abundant M17 protein nd 9.2 NYC At2g28120 MGNDETVRFL Nodulin-Like Protein nd 6.4 NYC At4g14360 MGQQQSQSKD Probable ankyrin nd 9.3 NYC At3g23280 MGQQQSKGEL Probable ankyrin nd 14.5 NYC At3g03810 MGSLVVHLSM putative auxin-independent growth promoter protein nd 1.0 NYC At2g17210 MGSHLCSKLQ Selenium-binding protein probable nd 0.5 PD At4g29380 MGNKIARTTQ Adaptor protein - H.sapiens nd 10.9 PD At4g15040 MGALPSKISY Cucumisin nd 9.9 PD At4g11310 MGSAKSAMLI Drought-inducible cysteine proteinase nd 1.7 PD At2g02250 MGHKQSVDSR Lectin-like protein nd 3.7 PD At2g02300 MGQKHGVDTR Phloem-specific lectin nd 2.8 PD At2g02310 MGQKLGVDSR Phloem-specific lectin probable nd 4.4 PD At1g03050 MGSSKFKRAI Putative protein destination factor putative nd 6.5 PD At4g29040 MGQGPSGGLN 26S proteasome AAA-ATPase subunit RPT2a PD At2g20140 MGQGPSGGLN 26S proteasome subunit PD At2g22310 MGAAGSKLEK Ubiquitin carboxyl terminal hydrolase probable PD At4g39910 MGAAGSKLEK Ubiquitin-specific protease (AtUBP3) PD At4g20110 MGLVNGRASL Vacuolar sorting receptor protein homolog nd 2.8 PD At1g10750 MGGVFVLRKP Carboxyl-terminal peptidase putative nd 0.4 PS At2g25840 MGHATSLSHF Trytophanyl-tRNA synthetase putative nd 0.2 PS At3g14600 MGGFRFHQYQ Putative 60S ribosomal protein L18A nd 0.3 T At1g52320 MGCAQSKIEN BZP-like protein T At1g02110 MGCTASKLDS BZP-like protein T At4g35240 MGCTSSKLDD BZP-like protein nd 10.2 T At1g21740 MGCGGSKVDD BZP-like protein nd 7.5 T At3g56170 MGNAIRLLRK Ca(2+)-dependent nuclease nd 2.5

6 T At2g23760 MGLATTTSSM Homeodomain transcription factor putative nd 0.5 T At5g04640 MGGVKRKISI MADS-box protein - like nd 2.8 T At3g12820 MGNRRAPCCD MYB-like DNA-binding protein probable nd 2.0 T At5g41360 MGNDERKRPT putative DNA repair protein and transcription factor nd 3.9 T At5g35805 MGAANVRRSL Reverse transcriptase. putative nd 3.7 T At1g14220 MGAKGCVNVL Ribonucleases T2 putative nd 2.3 T At3g09770 MGNISSSGGE RING zinc finger protein putative nd 8.3 T At4g00335 MGGCCSSSRK RING-H2 finger protein RHB1a nd 0.4 T At1g53670 MGSSSSSPKP Transcriptional regulator like protein nd 0.6 TF At3g47790 MGNMYSNSSG ABC-type transport protein probable nd 7.5 TF At5g36940 MGCLRSLVRR Cationic amino acid transporter-like protein nd 5.2 TF At5g12860 MGLGASVLTK Oxoglutarate/malate translocator -like protein TF At4g32500 MGIEKRKKMV Potassium channel - protein nd 0.4 TF At4g23700 MGTNGTTCPG Putative Na+/H+-exchanging protein nd 0.1 U At4g34320 MGNQTSKKSQ Hypothetical protein nd 17.4 U At4g34330 MGNKTSRKSK Hypothetical protein nd 16.6 U At1g65710 MGCCLSKKPS Hypothetical protein nd 14.0 U At1g21510 MGCCISKCSP Hypothetical protein nd 13.2 U At2g19090 MGCSHSKLDD Hypothetical protein nd 12.7 U At1g20530 MGCSPSKLDG Hypothetical protein nd 12.4 U At5g37010 MGCCLSKNPP Hypothetical protein nd 12.3 U At4g30130 MGCSHSKFDD Hypothetical protein nd 11.9 U At3g01085 MGCFNSKHSS Hypothetical protein nd 11.8 U At5g09260 MGNLFVKKPK Hypothetical protein nd 11.3 U At5g08660 MGSFCSKSLG Hypothetical protein nd 10.4 U At5g54480 MGIVASKGDS Hypothetical protein nd 10.0 U At2g43540 MGISGSKRVT Hypothetical protein nd 9.5 U At2g17110 MGCSTSKLDD Hypothetical protein nd 9.5 U At3g58400 MGNSVVRMDC Hypothetical protein nd 9.4 U At3g53410 MGNVISGGSR Hypothetical protein nd 9.1 U At5g02210 MGIISSRILK Hypothetical protein nd 9.0 U At2g41330 MGSSVSKTAS Hypothetical protein nd 8.9 U At3g49540 MGGCASRPKE Hypothetical protein nd 8.8 U At1g47510 MGNKNSMCGL Hypothetical protein nd 8.6 U At1g26360 MGNSFTCISH Hypothetical protein nd 8.6 U At3g23930 MGGCTSKQER Hypothetical protein nd 8.4 U At1g17940 MGCFASTPKD Hypothetical protein nd 8.3 U At3g03280 MGNYVSCALN Hypothetical protein nd 8.3 U At3g32904 MGNYRFKDPT Hypothetical protein nd 8.2 U At1g60880 MGGKKTKIEI Hypothetical protein nd 8.2 U At4g13540 MGGSTSKDER Hypothetical protein nd 8.1 U At3g50800 MGACASRESR Hypothetical protein nd 7.9 U At2g24320 MGNVTSNMAA Hypothetical protein nd 7.6 U At5g44900 MGASFSLFTD Hypothetical protein nd 7.5 U At1g18290 MGNTSSCAPL Hypothetical protein nd 7.3 U At4g31020 MGNVTSNVAA Hypothetical protein nd 7.3 U At5g57640 MGSINSVAEA Hypothetical protein nd 7.2 U At4g21920 MGNCICVTEK Hypothetical protein nd 7.2 U At2g34670 MGCAASRIDN Hypothetical protein nd 7.1 U At5g50090 MGNCQAVDTA Hypothetical protein nd 7.1

7 U At5g50830 MGCGGSRLGG Hypothetical protein nd 6.9 U At2g01790 MGNHQAVKKL Hypothetical protein nd 6.5 U At1g16960 MGCSSSRTIA Hypothetical protein nd 6.5 U At3g44160 MGAQKSIHAG Hypothetical protein nd 6.5 U At2g14350 MGNINFVKRI Hypothetical protein nd 6.3 U At3g59130 MGQGQSSDKE Hypothetical protein nd 5.9 U At5g20640 MGARSSQTVD Hypothetical protein nd 5.7 U At5g39430 MGTCMSTHSR Hypothetical protein nd 5.6 U At3g61610 MGQYATVWDQ Hypothetical protein nd 5.6 U At4g27480 MGLLSSVRSI Hypothetical protein nd 5.5 U At1g15540 MGGNETRELP Hypothetical protein nd 5.2 U At5g39590 MGASSSTDDK Hypothetical protein nd 5.1 U At2g35350 MGSGFSSLLP Hypothetical protein nd 5.0 U At4g34300 MGLSAARKLL Hypothetical protein nd 5.0 U At1g02940 MGINASHVPE Hypothetical protein nd 5.0 U At3g53000 MGSSLSNLND Hypothetical protein nd 5.0 U At4g25060 MGPTFSQLPL Hypothetical protein nd 4.9 U At5g37960 MGANRSIWND Hypothetical protein nd 4.9 U At1g47056 MGQSTSAAGN Hypothetical protein nd 4.8 U At5g26645 MGMKKVKLSL Hypothetical protein nd 4.4 U At5g27960 MGMKKVKLSL Hypothetical protein nd 4.4 U At5g57570 MGIASSVSMA Hypothetical protein nd 4.4 U At5g67620 MGNCQAAEAA Hypothetical protein nd 4.3 U At5g03890 MGNCLVMEKK Hypothetical protein nd 4.2 U At4g14650 MGNNNATAIL Hypothetical protein nd 4.2 U At3g52320 MGSSLCVAVR Hypothetical protein nd 4.1 U At3g31400 MGNEIGLGCE Hypothetical protein nd 3.9 U At3g09400 MGNGVASFSG Hypothetical protein nd 3.9 U At1g14060 MGITSSTDPK Hypothetical protein nd 3.8 U At3g60040 MGIGWSLHNR Hypothetical protein nd 3.8 U At1g59760 MGSVKRKSVE Hypothetical protein nd 3.7 U At3g54290 MGTCFSSSTK Hypothetical protein nd 3.4 U At5g38750 MGASSSTVQN Hypothetical protein nd 3.2 U At1g64700 MGNCLFGGLG Hypothetical protein nd 3.1 U At2g23920 MGNSIFGRCG Hypothetical protein nd 3.1 U At4g31370 MGLKASLSLL Hypothetical protein nd 3.0 U At1g08860 MGGCLSGDVK Hypothetical protein nd 2.9 U At4g10630 MGCVSSNLLN Hypothetical protein nd 2.9 U At1g60920 MGGTKRKIEM Hypothetical protein nd 2.8 U At3g21680 MGNCLRHDNG Hypothetical protein nd 2.8 U At2g47210 MGGTDAKDIL Hypothetical protein nd 2.7 U At1g46408 MGGVKRKIAI Hypothetical protein nd 2.4 U At4g34790 MGLMRSMLPN Hypothetical protein nd 2.4 U At2g32690 MGLISGKVCV Hypothetical protein nd 2.2 U At1g58170 MGSFLSFFLI Hypothetical protein nd 1.9 U At1g69650 MGASCSAPVS Hypothetical protein nd 1.8 U At2g25740 MGASVSTVYY Hypothetical protein nd 1.4 U At3g53070 MGTTDCRRHG Hypothetical protein nd 0.9 U At4g03292 MGLCKCNFDS Hypothetical protein nd 0.8 U At5g57200 MGTFTSFRKA Hypothetical protein nd 0.7 U At2g17160 MGTKARRPNP Hypothetical protein nd 0.6 U At1g13970 MGGCVSSQRK Hypothetical protein nd 0.3 U At5g55950 MGLVDSLLGK Hypothetical protein nd 0.3

8 U At4g00165 MGISKALRSL Hypothetical protein nd 0.3 U At2g24450 MGLKVSSSLL Hypothetical protein nd 0.3 U At5g58610 MGLFDCRVYR Hypothetical protein nd 0.1 U At2g06140 MGGPHSSHPY Hypothetical protein nd 0.1 U At5g40880 MGHQSSWMKQ Hypothetical protein nd 0.0 U At3g28600 MGNTFGSSLA Mitochondrial protein-like nd 5.4 U At3g08660 MGSDSTLSLP Non-Phototropic Hypocotyl Putative nd 1.8 U At5g06010 MGNKDSKGSS Unknown protein nd 14.0 U At2g46920 MGNGTSRVVG Unknown protein nd 13.4 U At2g44260 MGNCLSTSDP Unknown protein nd 12.6 U At3g01650 MGGGNSKEES Unknown protein nd 12.3 U At3g51290 MGCCQSRIDS Unknown protein nd 11.5 U At2g28890 MGNGIGKLSK Unknown protein nd 11.5 U At1g73390 MGCFASRPND Unknown protein nd 11.4 U At3g61920 MGNCVFKGNG Unknown protein nd 11.2 U At2g18630 MGGKSSKSKK Unknown protein nd 11.2 U At1g78420 MGNKLGRKRQ Unknown protein nd 11.1 U At5g25590 MGCAQSRVDN Unknown protein nd 11.0 U At5g63880 MGNLFVKKPQ Unknown protein nd 10.9 U At3g28850 MGCASSKHRK Unknown protein nd 10.8 U At3g27930 MGNALVKKEP Unknown protein nd 10.7 U At4g38690 MGSHLSKQLE Unknown protein nd 10.4 U At5g03200 MGNLISLIFC Unknown protein nd 10.2 U At1g05870 MGCVRCKSSD Unknown protein nd 10.2 U At2g30400 MGNYKFRISE Unknown protein nd 10.1 U At5g03870 MGCVSSKLGK Unknown protein nd 10.0 U At5g17350 MGNYVSSALS Unknown protein nd 9.8 U At5g54095 MGGCASKPKE Unknown protein nd 9.5 U At3g57070 MGSSASKTAS Unknown protein nd 9.5 U At5g45290 MGSGRSKSCL Unknown protein nd 9.5 U At1g67800 MGGSSSKESP Unknown protein U At3g18430 MGNTSSMLTQ Unknown protein nd 9.3 U At1g32190 MGCMFSHLAA Unknown protein nd 9.2 U At5g19080 MGISLSKRRR Unknown protein nd 9.2 U At5g06260 MGNSNSSSVD Unknown protein nd 9.0 U At1g34320 MGGLCSRSSS Unknown protein nd 8.8 U At4g39140 MGNACCVAAR Unknown protein nd 8.7 U At5g36800 MGMSKSKGNT Unknown protein nd 8.6 U At1g07850 MGNSSSSNTN Unknown protein nd 8.6 U At2g44230 MGNNSSAQSS Unknown protein nd 8.4 U At5g39865 MGCASSKNRN Unknown protein nd 8.3 U At1g09450 MGQRVSLAVY Unknown protein nd 8.3 U At5g14420 MGTGNSKENW Unknown protein nd 8.3 U At1g66480 MGNSITVKRK Unknown protein nd 8.3 U At3g26780 MGSSQSTQME Unknown protein nd 7.8 U At5g22210 MGNKATTVKE Unknown protein nd 7.8 U At1g63090 MGSGFSLFQK Unknown protein nd 7.5 U At1g60010 MGNCQAVDAA Unknown protein nd 7.3 U At5g66580 MGACASRESL Unknown protein nd 6.8 U At5g14020 MGCAQSTIAV Unknown protein nd 6.5 U At5g51570 MGNTYCILGG Unknown protein nd 6.5 U At1g04200 MGGVPSTPRK Unknown protein nd 6.5 U At1g22040 MGSVMSLSCS Unknown protein nd 6.5

9 U At1g34640 MGNEAALRSS Unknown protein nd 6.4 U At1g77500 MGCGGSKVDN Unknown protein nd 6.3 U At2g27090 MGASTSRIDE Unknown protein nd 6.2 U At1g10530 MGNCQAVNAA Unknown protein nd 6.2 U At1g76600 MGLCVSVNRN Unknown protein nd 6.0 U At5g07270 MGNSFGCSAS Unknown protein nd 5.9 U At2g40410 MGNALTFLYG Unknown protein nd 5.8 U At4g35230 MGCCQSLFSG Unknown protein nd 5.8 U At3g01290 MGNLFCCVLV Unknown protein nd 5.8 U At2g26190 MGLSLSLLLS Unknown protein nd 5.7 U At3g01520 MGSEPTKVMV Unknown protein nd 5.6 U At5g01800 MGGRFGVLLV Unknown protein nd 5.6 U At3g61930 MGNFLRRSCA Unknown protein nd 5.5 U At3g50080 MGQAPSSPAE Unknown protein nd 5.5 U At2g37970 MGMVFGKIAV Unknown protein nd 5.3 U At1g61563 MGMSKSIKVI Unknown protein nd 5.3 U At3g20340 MGNCLRHESE Unknown protein nd 5.2 U At1g59650 MGGCVSTPKS Unknown protein nd 5.0 U At1g13580 MGLLESVKSI Unknown protein nd 5.0 U At4g28180 MGCCFSAGKV Unknown protein nd 5.0 U At1g65510 MGSKTSTNMV Unknown protein nd 4.9 U At1g18680 MGSSFSASFT Unknown protein nd 4.8 U At1g10410 MGGCVSTPKS Unknown protein nd 4.8 U At1g69610 MGIVTTKTKT Unknown protein nd 4.8 U At1g12850 MGSSQSSQLL Unknown protein nd 4.7 U At4g10140 MGLSKGRLKA Unknown protein nd 4.6 U At3g25540 MGLFESVKSI Unknown protein nd 4.3 U At3g21400 MGQQLRRAVG Unknown protein nd 4.3 U At3g29180 MGGCVSTHSK Unknown protein nd 4.2 U At1g53400 MGCAGSTQSQ Unknown protein nd 4.2 U At5g67250 MGQAPSSTAE Unknown protein nd 4.0 U At3g57400 MGLISAKETK Unknown protein nd 4.0 U At2g01340 MGNSLGGKKT Unknown protein nd 3.6 U At5g62900 MGNCQAAEAA Unknown protein nd 3.6 U At1g04800 MGLIAGKVRV Unknown protein nd 3.5 U At1g21010 MGICVSFRRE Unknown protein nd 3.4 U At5g66290 MGASDSTLLG Unknown protein nd 3.4 U At2g23690 MGICSSYEST Unknown protein nd 3.3 U At4g00710 MGGQCSSLSC Unknown protein nd 3.3 U At2g25355 MGTEVSTSPT Unknown protein nd 3.1 U At2g01600 MGTLQSWRKA Unknown protein nd 3.1 U At5g60710 MGSKWRKAKV Unknown protein nd 3.1 U At3g62970 MGGSASLQSD Unknown protein nd 3.0 U At2g04800 MGSKPSSLAD Unknown protein nd 2.8 U At5g07910 MGCCASNTAG Unknown protein nd 2.6 U At4g23450 MGCCCCLPSI Unknown protein nd 2.5 U At1g04520 MGLSISFLSI Unknown protein nd 2.4 U At1g01240 MGAAEARALW Unknown protein nd 2.4 U At1g14910 MGTLQSWRRA Unknown protein nd 2.3 U At2g01490 MGITGSLTPD Unknown protein nd 2.2 U At2g28280 MGSGKTNRPS Unknown protein nd 2.2 U At3g61060 MGANISGGSP Unknown protein nd 2.2 U At3g55350 MGPIKTIKKK Unknown protein nd 2.2

10 U At1g61170 MGNCCVSSGT Unknown protein nd 2.0 U At1g11670 MGSEATTAVN Unknown protein nd 1.9 U At1g19530 MGSLMSGWDS Unknown protein nd 1.7 U At4g02090 MGNVAGSRKL Unknown protein nd 1.4 U At3g49070 MGIKVSSKIK Unknown protein nd 1.3 U At3g26380 MGLKFGIHVM Unknown protein nd 1.3 U At4g37240 MGICSSSEST Unknown protein nd 1.2 U At1g66860 MGNPSVNDLS Unknown protein nd 1.1 U At4g19985 MGLVGCVGKS Unknown protein nd 1.1 U At4g11020 MGNCLVGRNN Unknown protein nd 0.9 U At3g27210 MGSSSSSSLN Unknown protein nd 0.9 U At5g63905 MGCFSCFDGT Unknown protein nd 0.8 U At2g03810 MGISSSSGSF Unknown protein nd 0.7 U At2g43540 MGISSSSGSF Unknown protein nd 0.7 U At1g33350 MGTRVTQFSY Unknown protein nd 0.5 U At2g04900 MGNSVRSNLR Unknown protein nd 0.5 U At1g08800 MGSRSFTRAL Unknown protein nd 0.5 U At1g19680 MGSVCCVAAK Unknown protein nd 0.4 U At3g02290 MGCVSSCFRV Unknown protein nd 0.3 U At1g75400 MGSVCCVAVK Unknown protein nd 0.3 U At5g63670 MGSAPAQIPT Unknown protein nd 0.3 U At1g22360 MGSHVAQKQH Unknown protein nd 0.3 U At5g01840 MGNNYRFKLS Unknown protein nd 0.3 U At5g35220 MGTLTSVAFA Unknown protein nd 0.3 U At2g36630 MGLWNGKGTG Unknown protein nd 0.1

11 Putative Myristoylation site Prenylation site Entries Name ê Motif 1 Motif 2 Motif 3 ê AA At1g10630 ARFA1f MGLSFAKLFSRLFAK MVGLDAAGKTT VWDVGGQDK FANKQDLP RLLNS 186 At1g23490 ARFA1a MGLSFAKLFSRLFAK MVGLDAAGKTT VWDVGGQDK FANKQDLP IAGKA 181 At1g70490 ARFA1d MGLSFAKLFSRLFAK MVGLDAAGKTT VWDVGGQDK FANKQDLP IAGKA 181 At2g47170 ARFA1c MGLSFGKLFSRLFAK MVGLDAAGKTT VWDVGGQDK FANKQDLP IASKA 181 At3g62290 ARFA1e MGLSFGKLFSKLFAK MVGLDAAGKTT VWDVGGQDK FANKQDLP IANKA 181 At5g14670 ARFA1b MGLNFTKLFSRLFAK MVGLDAAGKTT VWDVGGQDK FANKQDLP LNIPF 188 At2g15310 ARFB1a MGARFSRIAKRFLPK MVGLDGSGKTT VWDIGGQEK FANKQDSR RGPRY 205 At3g03120 ARFB1c MGQTFRKLFDTFFGN MLGLDAAGKTT VWDVGGQEK FANKQDMR VGPSF 192 At5g17060 ARFB1b MGQAFRKLFDTFFGN MLGLDAAGKTT VWDVGGQEK FANKQDMR VGHSF 192 At2g24765 ARFE MGILFTRMFSSVFGN VLGLDNAGKTT VWDLGGQTS FANKQDLP KSGSG 182 At3g22950 ARFC1a MGAFMSRFWFMMFPA VVGLDNAGKTT VWDLGGQDR FANKQDLK GKATS 183 At1g02430 ARFD1a MGTALGKPFAGFFHQ LFGLDAAGKSS FWEMGGQQC FGNKHEVP NAERM 190 At1g02440 ARFD1b MGTTLGKPFAGFFHQ LFGLGGTGKSS FWEMGGQQC YGNKHEVP NAERM 190 At2g18390 ARLC1 MGLLSIIRKIKKKEK MVGLDNSGKTT IWDVGGQKT LANKQDIQ IYMLD 185 At3g49870 ARLA1b MGLLEAFLNWLRSLF LIGLQNAGKTS LWDLGGQPR LGNKIDKP SKSKN 184 At5g67560 ARLA1d MGLWDALLNWLRSLF LIGLQNAGKTS LWDLGGQPR LGNKIDKP SKSSN 184 At5g37680 ARLA1a MGLWDSLLNWLRSLF LVGLQNAGKTS IWDLGGQRR LGNKIDKS SRTAT 184 At2g33870 RABA1h MGTYKAEDDYDYLFK LTGDSGVGKSN IWDTAGQER VGNKADLN GCCAT 219 At1g28550 RABA1i MGAYRAEDDYDYLFK LTGDSGVGKSN IWDTAGQER VGNKADLR GCCSA 218 At4g18430 RABA1e MGAYRADDDYDYLFK LIGDSGVGKSN LWDTAGQER VGNKADLR GCCSG 217 At3g09910 RABC2b MGSSSGQSGYDLSFK LIGDSGVGKSS IWDTAGQEK VGNKVDRE RCCSS 205 At5g03530 RABC2a MGSSSGQSGYDLSFK LIGDSGVGKSS IWDTAGQER VGNKVDRE GCCS 210

12 At1g43890 RABC1 MGSSSGQPEFDYLFK LIGDSGVGKSS IWDTAGQER VGNKVDKE YCCSS 212 At3g54840 RABF1 MGCASSLPDRNSGTL LLGDSGVGKSC IWDTAGQER VGNKADLH PAPSS 202 At2g26300 GPA1 MGLLCSRSRHHTEDT LLGAGESGKST LFDVGGQRN FLNKFDIF EAGLL 383 Consensus MGXXX GXXXXGKS/T DXXGQ NKXD CC Fig.s1: Phylogenetic tree of all predicted GP in A. thaliana starting with G The three usual motifs (GXXXGKT/S; DXXG and NKXD) characteristic of GP are boxed. Sequences 1 to 12 correspond to ARF-like GP. whereas sequences 13 to 17 belong to RAB-like GP. as suggested by the presence of the SAK and C-terminal CCXX motifs