Nature Methods: doi: /nmeth Supplementary Figure 1. In vitro screening of recombinant R-CaMP2 variants.
|
|
|
- Shon Bell
- 5 years ago
- Views:
Transcription
1 Supplementary Figure 1 In vitro screening of recombinant R-CaMP2 variants. Baseline fluorescence compared to R-CaMP1.07 at nominally zero calcium plotted versus dynamic range ( F/F) for 150 recombinant candidate sensors (gray dots). As a reference, the values for R-GECO1 (orange), R-CaMP1.07 (blue), R-CaMP2 (black), and R- GECO2L (red) are indicated.
2 Supplementary Figure 2 Fluorescence spectra of R-CaMP2 and R-GECO2L. (a,b) Normalized fluorescence excitation (dashed lines) and emission (solid lines) spectra of Ca 2+ -free (bottom traces) and Ca 2+ - saturated (upper traces) R-CaMP2 (a) and R-GECO2L (b). The fluorescence intensity of each indicator was normalized to the peaks of the Ca 2+ -saturated spectra. (c) Two-photon cross-section (δ) spectra of R-CaMP2 in the Ca 2+ -free (bottom dotted traces) or Ca 2+ -saturated (upper solid traces) state. Error bar, s.e.m. (n = 3).
3 Supplementary Figure 3 ph titration of normalized fluorescence intensity and dynamic change ( F/F) of red GECIs. (a-c) ph titration of normalized fluorescence intensity of Ca 2+ -free (black) and Ca 2+ -saturated (red) states. The fluorescence intensity of each indicator was normalized to the peak of the calcium-saturated fluorescence intensity. Gray line indicates the dynamic range ( F/F). Experimental pk a values are summarized in Table 1. (a) R-GECO1 (b) R-CaMP1.07 (c) R-CaMP2
4 Supplementary Figure 4 Stopped-flow kinetic characterization of R-CaMP1.07 and R-CaMP2. Observed relaxation rate constants (K obs) plotted as a function of Ca 2+ concentrations. The association and dissociation rate constants and the Hill coefficients (K on, K off and n, respectively) were determined by fitting to the equation K obs = K on [Ca 2+ ] n + K off; these values are shown in Supplementary Table 2.
5 Supplementary Figure 5 Comparison of R-CaMP sensors during 1AP-triggered Ca 2+ imaging of synaptic boutons. (a) Representative image of synaptic boutons in cultured hippocampal neuron expressing R-CaMP2 (top) and R-CaMP1.07 (bottom), 50 msec after one field stimulus evoked one action potential. Left: EGFP (in monochrome). Right: Pseudocolor image of R-CaMP2 and R-CaMP1.07. Scale bar, 5 µm. (b) Single trials (gray) and trial-averaged responses of R-CaMP2 (black), R-GECO2L (red), and R-CaMP1.07 (blue) in response to a single AP. (c) Amplitude, SNR, rise times, and decay time constants for a single AP. Error bars, s.e.m. (n = 13, 8, 17 dishes sampled from 81, 52, 108 synaptic boutons for R-CaMP2, R-GECO2L, R-CaMP1.07, respectively, *P < 0.05, **P < 0.01, ***P < in Tukey s post-hoc test following one-way ANOVA. Individual P values: Amplitude, (R-CaMP2/R-GECO2L), (R-CaMP2/R- CaMP1.07); SNR, (R-CaMP2/R-GECO2L), (R-CaMP2/R-CaMP1.07); Rise time, (R-CaMP2/R- CaMP1.07).
6 Supplementary Figure 6 Comparison of R-CaMP2 and R-CaMP1.07 during a single Glu photolysis-induced somatic AP-triggered Ca 2+ imaging at the soma in cultured hippocampal neurons. (a) Single trials (n = 9, gray) and trial-averaged responses of R-CaMP2 (left, black) and R-CaMP1.07 (right, blue) in response to a single pulse of MNI-glutamate uncaging. (b) Amplitude, SNR, rise times, and decay time constants for a single pulse of MNI-glutamate uncaging. Error bars, s.e.m. (n = 9 cells each, *P < 0.05, ***P < in Welch s t-test. Individual P values: Amplitude, ; SNR, ; Rise time, ; Decay time,
7 Supplementary Figure 7 Comparison of R-CaMP2 and R-CaMP1.07 during a 1AP-evoked Ca 2+ transient in acute cortical slices. (a) Amplitude, (b) SNR, (c) rise times, and (d) decay time constants of somatic Ca 2+ transients induced by a single AP. Error bars, s.e.m. (n = 10 cells each, ***P < in Welch s t-test. Individual P values: Amplitude, ; SNR, ; Rise time, ; Decay time,
8 Supplementary Figure 8 Trial-averaged fluorescence transients of R-CaMP2 and R-CaMP1.07 to five spike trains delivered at different frequencies. (a,b) Trial-averaged data from experiments in Fig. 3d,e. Gray lines indicate individual sweeps (n = 10). Black and blue thick lines indicate average traces of R-CaMP2 (a) and R-CaMP1.07 (b). Right side of both figures: responses to 10, 20, and 40 Hz spike trains are shown in an expanded time scale. Stimulus time stamps are indicated by the vertical bars and dotted lines.
9 Supplementary Figure 9 Persistent R-CaMP2 expression does not affect passive membrane properties or Ca 2+ transient kinetics. (a) Representative traces of membrane potential deflections induced by current injection. Control (left, untransfected contralateral hemisphere), CAG-R-CaMP2 expressing neuron (right) (b) Resting membrane potential (left), membrane capacitance (middle), membrane input resistance (right) were unchanged between groups. Error bars, s.e.m. (n = 18, 17 cells for control (untransfected contralateral hemisphere) neuron, and CAG-R-CaMP2 expressing neuron, respectively, P = 0.824, 0.365, respectively, ns (not significant), Welch s t-test). (c) Representative traces of fluorescence changes ( F/F) in response to a single AP in neurons expressing either a TRE or a CAG promoter-driven R-CaMP2 in acute slices. Ten-trial average response of a single sweep each (gray) and averages of 10 sweeps (black) are overlaid (n = 10, 8 cells for TRE-R-CaMP2, CAG-R-CaMP2, respectively). (d) Either TRE or CAG promoter-driven R-CaMP2-expressing neurons showed undistinguishable single AP-evoked Ca 2+ transient kinetics.
10 Supplementary Figure 10 Ectopic R-CaMP2 expression has little effect on radial migration and does not cause toxic cell death. Mouse embryo were electroporated at E14 with a plasmid expressing control GCaMP6f (a,d: control) or R-CaMP2 (b,e: R-CaMP2), and sacrificed at E18 (a-c) or P18 (d-f). Coronal brain slices were imaged for baseline fluorescence (white) and immunostained with activated-caspase-3 (red). White arrowheads indicate labeled activated-caspase-3 cell. Scale bars, 100 µm (a,b) and 200 µm (d,e) respectively. (c,f) Quantification of the transfected cells that are activated-caspase-3 positive (Cas3+). Error bars, s.e.m. (n = 9182, 8242 cells for control (GCaMP6f), R-CaMP2 from 7 pairs of littermate animals (E18) for each condition, P = (c), ns (not significant), Welch s t- test). (n = 5171, 6302 cells for control (GCaMP6f), R-CaMP2 from 6 pairs of littermate animals (P18) for each condition, P = (f), ns (not significant), Welch s t-test).
11 Supplementary Figure 11 Simultaneous Ca 2+ imaging and electrophysiology in neocortical layer 2/3 neurons in vivo. (a) Representative traces of simultaneous recordings of calcium transients (top) and APs (bottom) in an R-CaMP2-expressing layer 2/3 neuron in the barrel cortex. The number of spikes for each burst is indicated below the trace. Scale bar, 5 µm. (b) Amplitude, SNR, and integral of calcium transients induced by number of APs in a 200 msec bin (n = 254, 115, 45, 26, 13 events for 1, 2, 3, 4, 5 action potentials, n = 9 cells in 7 mice). Error bars, s.e.m. (c) Median fluorescence change in response to a single action potential in vivo. Shading indicates s.e.m. (n = 9 cells). (d) The rise time and decay time constants of calcium transients in vivo induced by a single AP measured with cell-attached recording. Error bars, s.e.m.
12 Supplementary Figure 12 All-optical interrogation of an avoidance circuit using R-CaMP2 in freely moving C. elegans. (a) Schematic diagram of the ASH neuron-mediated avoidance circuit. (b) Representative R-CaMP2 response images in a freely moving transgenic animal jqex467, coexpressing R-CaMP2 (red) and EGFP (green) in AVA neurons (AVA::R-CaMP2), and expressing ChR2 in ASH neurons (ASH::ChR2). R-CaMP2 and EGFP signals were also weakly detected in a few other cells including AVE and AVD neurons. Arrowhead indicates AVA neuron. Bar 20 µm. (c) Behavioral responses of the jqex467 worms with or without all-trans-retinal (ATR). The observable backward response during the blue light illumination was counted. ***P < in Tukey s post-hoc test following one-way ANOVA. Individual P values: (Wild type without ATR/ASH::ChR2; AVA::R-CaMP2 with ATR), (with/without ATR in ATR/ASH::ChR2; AVA::R-CaMP2 background). (d) Causal relation between blue light-activated ASH neuronal activity (ChR2 on, light blue shade), AVA activity (R-CaMP2, blue trace) and backward movement (behavioral response). R-CaMP2 traces and backward responses of a jqex467 worm. Gray line shows a raw data of R-CaMP2/EGFP ratio image ( R/R) and black line shows a moving average of across 1 sec window.
13 Supplementary Table 1. Optical characteristics of R-CaMP1.07 and R-CaMP2. GECI Ca 2+ l abs a (ε b ) R-CaMP1.07 R-CaMP (22900) 577 (5320) 445 (7370) 563 (43800) 445 (28200) 576 (14200) 445 (16600) 563 (48100) l em c (Φ d ) Brightness e 592 (0.09) (0.20) (0.11) (0.23) a Absorbance peak (nm). b Extinction coefficient (M -1 cm -1 ). c Emission peak (nm). d Quantum yield. e Product of ε and Φ.
14 Supplementary Table 2. Kinetic characterization of R-CaMP1.07 and R-CaMP2 by stopped-flow photometry. GECI K on (M -n s -1 ) K off (s -1 ) n K d kinetic (nm) a K d static (nm) b R-CaMP R-CaMP a K d kinetic = (K off /K on ) 1/n. b From Figure 1c.
Nature Neuroscience: doi: /nn Supplementary Figure 1. Expression of GCaMP6s in LGN and their axons in V1.
Supplementary Figure 1 Expression of GCaMP6s in LGN and their axons in V1. (a, b) Coronal section of LGN and V1 expressing GCaMP6s. a Coronal slice (bregma -2.3 mm) including thalamus confirmed that GCaMP6s
Nature Neuroscience: doi: /nn Supplementary Figure 1. Amygdaloid complex and evoked synaptic currents recorded in CeM amygdala neurons.
Supplementary Figure 1 Amygdaloid complex and evoked synaptic currents recorded in CeM amygdala neurons. (a) Left: Schematic representation (modified from: Allen Brain Atlas) of coronal sections containing
National de la Recherche Scientifique and Université Paris Descartes, Paris, France.
FAST-RESPONSE CALMODULIN-BASED FLUORESCENT INDICATORS REVEAL RAPID INTRACELLULAR CALCIUM DYNAMICS Nordine Helassa a, Xiao-hua Zhang b, Ianina Conte a,c, John Scaringi b, Elric Esposito d, Jonathan Bradley
Supplementary Information. Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light
Supplementary Information Brain-wide D imaging of neuronal activity in Caenorhabditis elegans with sculpted light Tina Schrödel,, Robert Prevedel,2,,, Karin Aumayr,, Manuel Zimmer & Alipasha Vaziri,2,
Supplementary material: Orbitofrontal and striatal circuits dynamically encode the shift. between goal-directed and habitual actions
Supplementary material: Orbitofrontal and striatal circuits dynamically encode the shift between goal-directed and habitual actions Christina M. Gremel 1 & Rui M. Costa 1,2 1 Laboratory for Integrative
Waithe et al Supplementary Figures
Waithe et al Supplementary Figures Supplementary Figure 1 Expression and properties of WT and W391A mutant YFP- Ca V 2.2. A Immunoblot using Ca V 2.2 Ab for untransfected cells (UT, lane 1), YFP-Ca V 2.2
Neuronal Firing Sensitivity to Morphologic and Active Membrane Parameters
Neuronal Firing Sensitivity to Morphologic and Active Membrane Parameters Christina M. Weaver 1,2,3*, Susan L. Wearne 1,2,3* 1 Laboratory of Biomathematics, Mount Sinai School of Medicine, New York, New
SUPPLEMENTARY INFORMATION
Supplemental Figure S1. WISL is not elicited by courtship song and is dependent on chordotonal mechanosensory neurons. (a) Average locomotor velocity vs. time plots for wild-type CS flies exposed to recordings
Frequency (Hz) Amplitude (pa) D1 WT D1 KO D2 WT D2 KO D1 WT D1 KO D2 WT D2 KO
A D1 MSNs B D2 MSNs C Frequency (Hz) 4 3 2 1 D Amplitude (pa) 5 4 3 2 1 D1 D1 D2 D2 D1 D1 D2 D2 Supplemental Figure 1. B deletion did not alter GABA-mIPSCs in D1 or D2 MSNs. (A,B) Representative recording
Supplementary Figure 1: To test the role of mir-17~92 in orthologous genetic model of ADPKD, we generated Ksp/Cre;Pkd1 F/F (Pkd1-KO) and Ksp/Cre;Pkd1
Supplementary Figure 1: To test the role of mir-17~92 in orthologous genetic model of ADPKD, we generated Ksp/Cre;Pkd1 F/F (Pkd1-KO) and Ksp/Cre;Pkd1 F/F ;mir-17~92 F/F (Pkd1-miR-17~92KO) mice. (A) Q-PCR
Quantum Dot-Peptide-Fullerene Bioconjugates for Visualization of In Vitro and In Vivo Cellular Membrane Potential
Quantum Dot-Peptide-Fullerene Bioconjugates for Visualization of In Vitro and In Vivo Cellular Membrane Potential Okhil K. Nag, Michael H. Stewart, Jeffrey R. Deschamps, Kimihiro Susumu, Eunkeu Oh, Vassiliy
Consider the following spike trains from two different neurons N1 and N2:
About synchrony and oscillations So far, our discussions have assumed that we are either observing a single neuron at a, or that neurons fire independent of each other. This assumption may be correct in
Supplementary Figure 1
Supplementary Figure 1 Human cerebral vein characterization a, Superficial venous structures were identified and reconstructed using post-contrast brain MRI images (n=50). Scale bar, 3 cm. b-c, Vein diameters
Temporal whitening by power-law adaptation in neocortical neurons
Temporal whitening by power-law adaptation in neocortical neurons Christian Pozzorini, Richard Naud, Skander Mensi and Wulfram Gerstner School of Computer and Communication Sciences and School of Life
80% of all excitatory synapses - at the dendritic spines.
Dendritic Modelling Dendrites (from Greek dendron, tree ) are the branched projections of a neuron that act to conduct the electrical stimulation received from other cells to and from the cell body, or
Microsystems for Neuroscience and Medicine. Lecture 9
1 Microsystems for Neuroscience and Medicine Lecture 9 2 Neural Microsystems Neurons - Structure and behaviour Measuring neural activity Interfacing with neurons Medical applications - DBS, Retinal Implants
Nature Neuroscience: doi: /nn.2283
Supplemental Material for NN-A2678-T Phase-to-rate transformations encode touch in cortical neurons of a scanning sensorimotor system by John Curtis and David Kleinfeld Figure S. Overall distribution of
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION Supplementary Figure S1. Pulses >3mJ reduce membrane resistance in HEK cells. Reversal potentials in a representative cell for IR-induced currents with laser pulses of 0.74 to
Supplementary information for. Observation of photovoltaic action from photoacid-modified Nafion due to light-driven ion transport
Supplementary information for Observation of photovoltaic action from photoacid-modified Nafion due to light-driven ion transport William White, a# Christopher D. Sanborn, a# Ronald S. Reiter, a David
The homogeneous Poisson process
The homogeneous Poisson process during very short time interval Δt there is a fixed probability of an event (spike) occurring independent of what happened previously if r is the rate of the Poisson process,
Frequency Adaptation and Bursting
BioE332A Lab 3, 2010 1 Lab 3 January 5, 2010 Frequency Adaptation and Bursting In the last lab, we explored spiking due to sodium channels. In this lab, we explore adaptation and bursting due to potassium
Simultaneous intracellular chloride and ph measurements using a GFPbased
nature methods Simultaneous intracellular chloride and ph measurements using a GFPbased sensor Daniele Arosio, Fernanda Ricci, Laura Marchetti, Roberta Gualdani, Lorenzo Albertazzi & Fabio Beltram Supplementary
This script will produce a series of pulses of amplitude 40 na, duration 1ms, recurring every 50 ms.
9.16 Problem Set #4 In the final problem set you will combine the pieces of knowledge gained in the previous assignments to build a full-blown model of a plastic synapse. You will investigate the effects
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2215 Figure S1 Number of egfp-vps4a bursts versus cellular expression levels. The total number of egfp-vps4a bursts, counted at the end of each movie (frame 2000, after 1h 28 min) are plotted
SUPPLEMENTARY INFORMATION
doi:10.1038/nature09714 Supplementary Figure 1 The mouse olfactory system and summary of our results. a, Schematic of the mouse olfactory system (sagittal view). See text for more details. The red and
High-dimensional geometry of cortical population activity. Marius Pachitariu University College London
High-dimensional geometry of cortical population activity Marius Pachitariu University College London Part I: introduction to the brave new world of large-scale neuroscience Part II: large-scale data preprocessing
Encoding of Both Analog- and Digital-like Behavioral Outputs by One C. elegans Interneuron
Article Encoding of Both Analog- and Digital-like Behavioral Outputs by One C. elegans Interneuron Zhaoyu Li, Jie Liu, Maohua Zheng, and X.Z. Shawn Xu, * Life Sciences Institute and Department of Molecular
SUPPLEMENTARY INFORMATION
doi:10.1038/nature11419 Supplementary Figure 1 Schematic representation of innate immune signaling pathways induced by intracellular Salmonella in cultured macrophages. a, During the infection Salmonella
CJ LI LRRK2 MOUSE COMPARISON STUDY. Phenotyping Data Results
CJ LI MOUSE COMPARISON STUDY Phenotyping Data Results NOTE FOR USE This comparison study was run as an opportunistic look at the phenotype of multiple CJ Li-generated mouse lines. The primary purpose of
File Name: Supplementary Information Description: Supplementary Figures and Supplementary Note. File Name: Peer Review File Description:
File Name: Supplementary Information Description: Supplementary Figures and Supplementary Note File Name: Peer Revie File Description: Supplementary Fig.1. Complete set of ACFs extracted by FLCS analysis.
Dynamic Models for Passive Components
PCB Design 007 QuietPower columns Dynamic Models for Passive Components Istvan Novak, Oracle, February 2016 A year ago the QuietPower column [1] described the possible large loss of capacitance in Multi-Layer
Internally generated preactivation of single neurons in human medial frontal cortex predicts volition
Internally generated preactivation of single neurons in human medial frontal cortex predicts volition Itzhak Fried, Roy Mukamel, Gabriel Kreiman List of supplementary material Supplementary Tables (2)
Regulation of interneuron excitability by gap junction coupling with principal cells
Regulation of interneuron excitability by gap junction coupling with principal cells Pierre F Apostolides 1,2 & Laurence O Trussell 2 Electrical coupling of inhibitory interneurons can synchronize activity
Nature Protocols: doi: /nprot Supplementary Figure 1
Supplementary Figure 1 Photographs of the 3D-MTC device and the confocal fluorescence microscopy. I: The system consists of a Leica SP8-Confocal microscope (with an option of STED), a confocal PC, a 3D-MTC
SUPPLEMENTARY INFORMATION
doi:10.1038/nature10923 Supplementary Figure 1 Ten-a and Ten-m antibody and cell type specificities. a c, Representative single confocal sections of a Drosophila NMJ stained with antibodies to Ten-a (red),
Figure S1. Programmed cell death in the AB lineage occurs in temporally distinct
SUPPLEMENTAL FIGURE LEGENDS Figure S1. Programmed cell death in the AB lineage occurs in temporally distinct waves. (A) A representative sub-lineage (ABala) of the C. elegans lineage tree (adapted from
Biological Modeling of Neural Networks
Week 4 part 2: More Detail compartmental models Biological Modeling of Neural Networks Week 4 Reducing detail - Adding detail 4.2. Adding detail - apse -cable equat Wulfram Gerstner EPFL, Lausanne, Switzerland
Modeling of Retinal Ganglion Cell Responses to Electrical Stimulation with Multiple Electrodes L.A. Hruby Salk Institute for Biological Studies
Modeling of Retinal Ganglion Cell Responses to Electrical Stimulation with Multiple Electrodes L.A. Hruby Salk Institute for Biological Studies Introduction Since work on epiretinal electrical stimulation
Imagent for fnirs and EROS measurements
TECHNICAL NOTE Imagent for fnirs and EROS measurements 1. Brain imaging using Infrared Photons Brain imaging techniques can be broadly classified in two groups. One group includes the techniques that have
DOI: 10.1038/ncb2819 Gαi3 / Actin / Acetylated Tubulin Gαi3 / Actin / Acetylated Tubulin a a Gαi3 a Actin Gαi3 WT Gαi3 WT Gαi3 WT b b Gαi3 b Actin Gαi3 KO Gαi3 KO Gαi3 KO # # Figure S1 Loss of protein
T H E J O U R N A L O F C E L L B I O L O G Y
Supplemental material Kasprowicz et al., http://www.jcb.org/cgi/content/full/jcb.201310090/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. NMJ morphology of shi 12-12B ; shi-4c not treated
Supplementary information
This journal is The Royal Society of Chemistry 0 Supplementary information Pattern type Area (cm ) Hybrid 0 0 0.00 Hybrid 0 0 0.00 Hybrid 0 0 0.00 Line 00 µm 0.00 Line 0 µm 0.00 Line 0 µm 0.00 Line 0 µm
An Efficient Method for Computing Synaptic Conductances Based on a Kinetic Model of Receptor Binding
NOTE Communicated by Michael Hines An Efficient Method for Computing Synaptic Conductances Based on a Kinetic Model of Receptor Binding A. Destexhe Z. F. Mainen T. J. Sejnowski The Howard Hughes Medical
PHYSICS 171 UNIVERSITY PHYSICS LAB II. Experiment 6. Transient Response of An RC Circuit
PHYSICS 171 UNIVERSITY PHYSICS LAB II Experiment 6 Transient Response of An RC Circuit Equipment: Supplies: Function Generator, Dual Trace Oscilloscope.002 Microfarad, 0.1 Microfarad capacitors; 1 Kilohm,
Neocortical Pyramidal Cells Can Control Signals to Post-Synaptic Cells Without Firing:
Neocortical Pyramidal Cells Can Control Signals to Post-Synaptic Cells Without Firing: a model of the axonal plexus Erin Munro Department of Mathematics Boston University 4/14/2011 Gap junctions on pyramidal
Supplementary Figure S1. The maximum possible short circuit current (J sc ) from a solar cell versus the absorber band-gap calculated assuming 100%
Supplementary Figure S1. The maximum possible short circuit current (J sc ) from a solar cell versus the absorber band-gap calculated assuming 100% (black) and 80% (red) external quantum efficiency (EQE)
Lecture 6: Non-Cortical Visual Pathways MCP 9.013/7.68, 03
Lecture 6: Non-Cortical Visual Pathways MCP 9.013/7.68, 03 Roger W. Sperry The problem of central nervous reorganization after nerve regeneration and muscle transposition. R.W. Sperry. Quart. Rev. Biol.
Model Answer (Paper code: AR-7112) M. Sc. (Physics) IV Semester Paper I: Laser Physics and Spectroscopy
Model Answer (Paper code: AR-7112) M. Sc. (Physics) IV Semester Paper I: Laser Physics and Spectroscopy Section I Q1. Answer (i) (b) (ii) (d) (iii) (c) (iv) (c) (v) (a) (vi) (b) (vii) (b) (viii) (a) (ix)
Triplets of Spikes in a Model of Spike Timing-Dependent Plasticity
The Journal of Neuroscience, September 20, 2006 26(38):9673 9682 9673 Behavioral/Systems/Cognitive Triplets of Spikes in a Model of Spike Timing-Dependent Plasticity Jean-Pascal Pfister and Wulfram Gerstner
devoted to the photoelectric effect, allows you to
1 Quantum Theory & Atomic Structure: PhET http://phet.colorado.edu/simulations/sims.php?sim=wave_on_a_string Wave on a String, (Mark Hayden already uses this one), shows a string attached at one end, and
In#uence of dendritic topologyon "ring patterns in model neurons
Neurocomputing 38}40 (2001) 183}189 In#uence of dendritic topologyon "ring patterns in model neurons Jacob Duijnhouwer, Michiel W.H. Remme, Arjen van Ooyen*, Jaap van Pelt Netherlands Institute for Brain
Dendritic computation
Dendritic computation Dendrites as computational elements: Passive contributions to computation Active contributions to computation Examples Geometry matters: the isopotential cell Injecting current I
Synchrony in Neural Systems: a very brief, biased, basic view
Synchrony in Neural Systems: a very brief, biased, basic view Tim Lewis UC Davis NIMBIOS Workshop on Synchrony April 11, 2011 components of neuronal networks neurons synapses connectivity cell type - intrinsic
The Diffusion Coefficient for PGK Folding in Eukaryotic Cells
Biophysical Journal, Volume 99 Supporting Material The Diffusion Coefficient for PGK Folding in Eukaryotic Cells Apratim Dhar, Simon Ebbinghaus, Zhen Shen, Tripta Mishra, and Martin Gruebele 1 Supplementary
SUPPLEMENTARY INFORMATION
Figure S1 Multiplicative scaling of the granule cell input-output relation is not dependent on input rate. Input-output relations with short-term depression (STD) from Fig. 1d after normalizing by the
The functional organization of the visual cortex in primates
The functional organization of the visual cortex in primates Dominated by LGN M-cell input Drosal stream for motion perception & spatial localization V5 LIP/7a V2 V4 IT Ventral stream for object recognition
Neuron, volume 60 Supplemental Data
Neuron, volume 60 Supplemental Data Tuning of Synaptic Integration in the Medial Entorhinal Cortex to the Organization of Grid Cell Firing Fields Derek L.F. Garden, Paul D. Dodson, Cian O Donnell, Melanie
SUPPLEMENTARY INFORMATION
Supplementary Information Speckle-free laser imaging using random laser illumination Brandon Redding 1*, Michael A. Choma 2,3*, Hui Cao 1,4* 1 Department of Applied Physics, Yale University, New Haven,
Supplementary Materials for
advances.sciencemag.org/cgi/content/full/1/9/e1500511/dc1 Supplementary Materials for Contractility parameters of human -cardiac myosin with the hypertrophic cardiomyopathy mutation R403Q show loss of
Supplementary Figure 1. Characterization of the single-photon quantum light source based on spontaneous parametric down-conversion (SPDC).
.2 Classical light source.8 g (2) ().6.4.2 EMCCD SPAD 2 3.2.4.6.8..2.4.6.8.2 Mean number of photon pairs per pump pulse 4 5 6 7 8 9 2 3 4 Supplementary Figure. Characterization of the single-photon quantum
Ultrafast Dynamics and Single Particle Spectroscopy of Au-CdSe Nanorods
Supporting Information Ultrafast Dynamics and Single Particle Spectroscopy of Au-CdSe Nanorods G. Sagarzazu a, K. Inoue b, M. Saruyama b, M. Sakamoto b, T. Teranishi b, S. Masuo a and N. Tamai a a Department
Supplementary Figures
Supplementary Figures Supplementary Figure 1. AFM profiles of the charge transport and perovskite layers. AFM Image showing the thickness (y axis) of the layer with respect to the horizontal position of
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:1.138/nature1237 a b retinol retinal RA OH RDH (retinol dehydrogenase) O H Raldh2 O R/R.6.4.2 (retinaldehyde dehydrogenase 2) RA retinal retinol..1.1 1 Concentration (nm)
Cell Death & Trophic Factors II. Steven McLoon Department of Neuroscience University of Minnesota
Cell Death & Trophic Factors II Steven McLoon Department of Neuroscience University of Minnesota 1 Remember? Neurotrophins are cell survival factors that neurons get from their target cells! There is a
Figure 1: Graphene release, transfer and stacking processes. The graphene stacking began with CVD
Supplementary figure 1 Graphene Growth and Transfer Graphene PMMA FeCl 3 DI water Copper foil CVD growth Back side etch PMMA coating Copper etch in 0.25M FeCl 3 DI water rinse 1 st transfer DI water 1:10
Nature Biotechnology: doi: /nbt Supplementary Figure 1. Overexpression of YFP::GPR-1 in the germline.
Supplementary Figure 1 Overexpression of YFP::GPR-1 in the germline. The pie-1 promoter and 3 utr were used to express yfp::gpr-1 in the germline. Expression levels from the yfp::gpr-1(cai 1.0)-expressing
Supplementary Figure 1. Markedly decreased numbers of marginal zone B cells in DOCK8 mutant mice Supplementary Figure 2.
Supplementary Figure 1. Markedly decreased numbers of marginal zone B cells in DOCK8 mutant mice. Percentage of marginal zone B cells in the spleen of wild-type mice (+/+), mice homozygous for cpm or pri
A realistic neocortical axonal plexus model has implications for neocortical processing and temporal lobe epilepsy
A realistic neocortical axonal plexus model has implications for neocortical processing and temporal lobe epilepsy Neocortical Pyramidal Cells Can Send Signals to Post-Synaptic Cells Without Firing Erin
Supplemental Information. C. elegans AWA Olfactory Neurons Fire. Calcium-Mediated All-or-None Action Potentials
Cell, Volume 175 Supplemental Information C. elegans AWA Olfactory Neurons Fire Calcium-Mediated All-or-None Action Potentials Qiang Liu, Philip B. Kidd, May Dobosiewicz, and Cornelia I. Bargmann Table
Supporting Online Material for
www.sciencemag.org/cgi/content/full/319/5869/1543/dc1 Supporting Online Material for Synaptic Theory of Working Memory Gianluigi Mongillo, Omri Barak, Misha Tsodyks* *To whom correspondence should be addressed.
University of California, Davis One Shields Ave, Davis, University of California, Davis One Shields Ave, Davis, CA, 95616
Supporting information for Noncanonical Photodynamics of the Orange/Green Cyanobacteriochrome Power Sensor NpF264g7 from the PtxD Phototaxis Regulator of Nostoc punctiforme Julia S. Kirpich, a# Che-Wei
Supplementary Information: Three-dimensional quantum photonic elements based on single nitrogen vacancy-centres in laser-written microstructures
Supplementary Information: Three-dimensional quantum photonic elements based on single nitrogen vacancy-centres in laser-written microstructures Andreas W. Schell, 1, a) Johannes Kaschke, 2 Joachim Fischer,
Exercises. Chapter 1. of τ approx that produces the most accurate estimate for this firing pattern.
1 Exercises Chapter 1 1. Generate spike sequences with a constant firing rate r 0 using a Poisson spike generator. Then, add a refractory period to the model by allowing the firing rate r(t) to depend
Neural Encoding I: Firing Rates and Spike Statistics
Chapter 1 Neural Encoding I: Firing Rates and Spike Statistics 1.1 Introduction Neurons are remarkable among the cells of the body in their ability to propagate signals rapidly over large distances. They
Introduction and summary of the chapters
Introduction and summary of the chapters 1. Electroreception Electroreception is the ability of animal species to detect weak electric fields. It is mediated by a sensory system that occurs in some aquatic
PHYSICS nd TERM Outline Notes (continued)
PHYSICS 2800 2 nd TERM Outline Notes (continued) Section 6. Optical Properties (see also textbook, chapter 15) This section will be concerned with how electromagnetic radiation (visible light, in particular)
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering Lu Wei, Fanghao Hu, Yihui Shen, Zhixing Chen, Yong Yu, Chih-Chun Lin, Meng C. Wang, Wei Min * * To whom correspondence
Supplementary Figure 1
Supplementary Figure 1 Single-cell RNA sequencing reveals unique sets of neuropeptides, transcription factors and receptors in specific types of sympathetic neurons (a) Dissection of paravertebral SGs
Integration of synaptic inputs in dendritic trees
Integration of synaptic inputs in dendritic trees Theoretical Neuroscience Fabrizio Gabbiani Division of Neuroscience Baylor College of Medicine One Baylor Plaza Houston, TX 77030 e-mail:gabbiani@bcm.tmc.edu
Role of Mitochondrial Remodeling in Programmed Cell Death in
Developmental Cell, Vol. 12 Supplementary Data Role of Mitochondrial Remodeling in Programmed Cell Death in Drosophila melanogaster Gaurav Goyal, Brennan Fell, Apurva Sarin, Richard J. Youle, V. Sriram.
Conditions and Constraints for Astrocyte Calcium Signaling in the Hippocampal Mossy Fiber Pathway
Article Conditions and Constraints for Astrocyte Calcium Signaling in the Hippocampal Mossy Fiber Pathway Martin D. Haustein, 1 Sebastian Kracun, 1 Xiao-Hong Lu, 3 Tiffany Shih, 4 Olan Jackson-Weaver,
This document is downloaded from DR-NTU, Nanyang Technological University Library, Singapore.
This document is downloaded from DR-NTU, Nanyang Technological University Library, Singapore. Title Small-signal neural models and their applications Author(s) Basu, Arindam Citation Basu, A. (01). Small-signal
Nature Neuroscience: doi: /nn.2717
Supplementary Fig. 1. Dendrite length is not secondary to body length. Dendrite growth proceeds independently of the rate of body growth and decreases in rate in adults. n 20 on dendrite measurement, n
Single Photon detectors
Single Photon detectors Outline Motivation for single photon detection Semiconductor; general knowledge and important background Photon detectors: internal and external photoeffect Properties of semiconductor
Synaptic Input. Linear Model of Synaptic Transmission. Professor David Heeger. September 5, 2000
Synaptic Input Professor David Heeger September 5, 2000 The purpose of this handout is to go a bit beyond the discussion in Ch. 6 of The Book of Genesis on synaptic input, and give some examples of how
Supplementary Figure 1. SDS-PAGE analysis of GFP oligomer variants with different linkers. Oligomer mixtures were applied to a PAGE gel containing
Supplementary Figure 1. SDS-PAGE analysis of GFP oligomer variants with different linkers. Oligomer mixtures were applied to a PAGE gel containing 0.1% SDS without boiling. The gel was analyzed by a fluorescent
State-Space Methods for Inferring Spike Trains from Calcium Imaging
State-Space Methods for Inferring Spike Trains from Calcium Imaging Joshua Vogelstein Johns Hopkins April 23, 2009 Joshua Vogelstein (Johns Hopkins) State-Space Calcium Imaging April 23, 2009 1 / 78 Outline
Supporting Information. Probing DNA Translocations with Inplane Current Signals in a Graphene Nanoribbon with a Nanopore
Supporting Information Probing DNA Translocations with Inplane Current Signals in a Graphene Nanoribbon with a Nanopore Stephanie J. Heerema, Leonardo Vicarelli, Sergii Pud, Raymond N. Schouten, Henny
Measurement Examples. Excitation and Emission Scans. Steady State Fluorescence Anisotropy. Kinetic Measurements
Measurement Examples A division of Edinburgh Instruments Ltd. Excitation and Emission Scans Excitation and emission spectra are standard measurements in fluorescence spectroscopy. The figure demonstrates
Every animal is represented by a blue circle. Correlation was measured by Spearman s rank correlation coefficient (ρ).
Supplementary Figure 1 Correlations between tone and context freezing by animal in each of the four groups in experiment 1. Every animal is represented by a blue circle. Correlation was measured by Spearman
Motile Axonal Mitochondria Contribute to the Variability of Presynaptic Strength
Cell Reports Report Motile Axonal Mitochondria Contribute to the Variability of Presynaptic Strength Tao Sun, 1,2 Haifa Qiao, 1,2,3 Ping-Yue Pan, 1,4 Yanmin Chen, 1 and Zu-Hang Sheng 1, * 1 Synaptic Functions
AT2 Neuromodeling: Problem set #3 SPIKE TRAINS
AT2 Neuromodeling: Problem set #3 SPIKE TRAINS Younesse Kaddar PROBLEM 1: Poisson spike trains Link of the ipython notebook for the code Brain neuron emit spikes seemingly randomly: we will aim to model
Emergence of resonances in neural systems: the interplay between adaptive threshold and short-term synaptic plasticity
Emergence of resonances in neural systems: the interplay between adaptive threshold and short-term synaptic plasticity Jorge F. Mejias 1,2 and Joaquín J. Torres 2 1 Department of Physics and Center for
Adaptation in the Neural Code of the Retina
Adaptation in the Neural Code of the Retina Lens Retina Fovea Optic Nerve Optic Nerve Bottleneck Neurons Information Receptors: 108 95% Optic Nerve 106 5% After Polyak 1941 Visual Cortex ~1010 Mean Intensity
Lecture 11 : Simple Neuron Models. Dr Eileen Nugent
Lecture 11 : Simple Neuron Models Dr Eileen Nugent Reading List Nelson, Biological Physics, Chapter 12 Phillips, PBoC, Chapter 17 Gerstner, Neuronal Dynamics: from single neurons to networks and models
SUPPLEMENTARY INFORMATION
Fast spin information transfer between distant quantum dots using individual electrons B. Bertrand, S. Hermelin, S. Takada, M. Yamamoto, S. Tarucha, A. Ludwig, A. D. Wieck, C. Bäuerle, T. Meunier* Content
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2647 Figure S1 Other Rab GTPases do not co-localize with the ER. a, Cos-7 cells cotransfected with an ER luminal marker (either KDEL-venus or mch-kdel) and mch-tagged human Rab5 (mch-rab5,
Supplementary material
Supplementary material Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins Vladimir V. Rogov 1,*, Hironori Suzuki 2,3,*, Mija Marinković 4, Verena Lang
Spike-Frequency Adaptation: Phenomenological Model and Experimental Tests
Spike-Frequency Adaptation: Phenomenological Model and Experimental Tests J. Benda, M. Bethge, M. Hennig, K. Pawelzik & A.V.M. Herz February, 7 Abstract Spike-frequency adaptation is a common feature of
Phase Response. 1 of of 11. Synaptic input advances (excitatory) or delays (inhibitory) spiking
Printed from the Mathematica Help Browser 1 1 of 11 Phase Response Inward current-pulses decrease a cortical neuron's period (Cat, Layer V). [Fetz93] Synaptic input advances (excitatory) or delays (inhibitory)